AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0260190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0260190 MtrunA17_Chr2g0317741 89.041 73 8 0 1 73 1 73 1.49e-37 119
Msa0260190 MtrunA17_Chr2g0317751 66.216 74 22 2 1 73 1 72 3.82e-30 101
Msa0260190 MtrunA17_Chr8g0385311 36.000 75 45 2 1 73 1 74 7.03e-12 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGAAACATTGAAATTTA+TGG 0.161117 2_2:+57720192 None:intergenic
ATTATATTCATCACTAAGTA+TGG 0.332590 2_2:-57721336 Msa0260190:exon
ATATGATAAAAGTGCATAAC+TGG 0.354165 2_2:+57719956 None:intergenic
AGCAAGGACTTGTGAGCATT+TGG 0.402994 2_2:-57720300 Msa0260190:CDS
GAACGAGGAAGAGGAAGCAC+AGG 0.435069 2_2:+57721294 None:intergenic
CTTGCAAGTTGCATGCATCT+TGG 0.455133 2_2:+57720124 None:intergenic
AACAAAGCACACTTAATAAG+TGG 0.478955 2_2:-57720221 Msa0260190:CDS
CGACAAAGAGAACGAGGAAG+AGG 0.484363 2_2:+57721285 None:intergenic
TTAAGTGTTTGTGTCTAATG+TGG 0.522872 2_2:+57721387 None:intergenic
CATTTGGCTGATACATATAG+AGG 0.536909 2_2:-57720284 Msa0260190:CDS
AGTTGCATGCATCTTGGTGT+TGG 0.540849 2_2:+57720130 None:intergenic
ATGGAGAAGAGATCAGTAGC+TGG 0.575142 2_2:-57721317 Msa0260190:CDS
ACGATATTGATGAGTACCTA+TGG 0.577282 2_2:+57719989 None:intergenic
CATCTATGAATTATGTCCAT+AGG 0.585201 2_2:-57720005 Msa0260190:three_prime_UTR
GCACTCAAAACTGTTAAGTG+TGG 0.610534 2_2:-57720172 Msa0260190:exon
AGACACATATATAGCCCCAA+TGG 0.623961 2_2:+57721431 None:intergenic
CAGCTAGCATCTGTGAAGCA+TGG 0.627719 2_2:+57720260 None:intergenic
CATGGATGCAGAAGAAATTG+TGG 0.636627 2_2:-57720330 Msa0260190:intron
AAATTGTGGTGATTGAAGCA+AGG 0.641556 2_2:-57720316 Msa0260190:CDS
AAACTGTTAAGTGTGGAATG+TGG 0.677412 2_2:-57720165 Msa0260190:exon
AAACACTTAACACATAAGTG+AGG 0.690567 2_2:-57721374 Msa0260190:five_prime_UTR
TACGTGCGACAAAGAGAACG+AGG 0.732491 2_2:+57721279 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTCTAATATTGTTTAA+TGG - chr2_2:57720990-57721009 Msa0260190:intron 10.0%
!! TATATTCTAATATTGTTTAA+TGG - chr2_2:57720990-57721009 Msa0260190:intron 10.0%
!!! TCTTTCTCTAATTTAATTTT+TGG - chr2_2:57720294-57720313 Msa0260190:CDS 15.0%
!! CTTAATTAACAATATACTAA+AGG + chr2_2:57720411-57720430 None:intergenic 15.0%
!!! TAATTAAGTTTTTTTATAGC+AGG - chr2_2:57720423-57720442 Msa0260190:intron 15.0%
!! AAAAAAAAAGACAAATTGAA+CGG - chr2_2:57720497-57720516 Msa0260190:intron 15.0%
!! TATATAAATATTACTTTGTC+AGG + chr2_2:57720645-57720664 None:intergenic 15.0%
!!! TCTTTCTCTAATTTAATTTT+TGG - chr2_2:57720294-57720313 Msa0260190:CDS 15.0%
!! CTTAATTAACAATATACTAA+AGG + chr2_2:57720411-57720430 None:intergenic 15.0%
!!! TAATTAAGTTTTTTTATAGC+AGG - chr2_2:57720423-57720442 Msa0260190:intron 15.0%
!! AAAAAAAAAGACAAATTGAA+CGG - chr2_2:57720497-57720516 Msa0260190:intron 15.0%
!! TATATAAATATTACTTTGTC+AGG + chr2_2:57720645-57720664 None:intergenic 15.0%
!! ATTATATTCATCACTAAGTA+TGG - chr2_2:57720009-57720028 Msa0260190:three_prime_UTR 20.0%
!! AAAGTATATCTTAAATTTCG+TGG - chr2_2:57720197-57720216 Msa0260190:CDS 20.0%
!! AGCAACAATAATAATTAGAT+GGG + chr2_2:57720347-57720366 None:intergenic 20.0%
!! ATTAACAATATACTAAAGGA+AGG + chr2_2:57720407-57720426 None:intergenic 20.0%
!! AAATAAAAAATCGACACATT+TGG + chr2_2:57720609-57720628 None:intergenic 20.0%
!!! TGTCGATTTTTTATTTGTAA+AGG - chr2_2:57720613-57720632 Msa0260190:intron 20.0%
!!! ATTTTTTATTTGTAAAGGTC+AGG - chr2_2:57720618-57720637 Msa0260190:intron 20.0%
!! TAATATTGTTTAATGGTACA+TGG - chr2_2:57720997-57721016 Msa0260190:intron 20.0%
!! ATTATATTCATCACTAAGTA+TGG - chr2_2:57720009-57720028 Msa0260190:three_prime_UTR 20.0%
!! AAAGTATATCTTAAATTTCG+TGG - chr2_2:57720197-57720216 Msa0260190:CDS 20.0%
!! AGCAACAATAATAATTAGAT+GGG + chr2_2:57720347-57720366 None:intergenic 20.0%
!! ATTAACAATATACTAAAGGA+AGG + chr2_2:57720407-57720426 None:intergenic 20.0%
!! AAATAAAAAATCGACACATT+TGG + chr2_2:57720609-57720628 None:intergenic 20.0%
!!! TGTCGATTTTTTATTTGTAA+AGG - chr2_2:57720613-57720632 Msa0260190:intron 20.0%
!!! ATTTTTTATTTGTAAAGGTC+AGG - chr2_2:57720618-57720637 Msa0260190:intron 20.0%
!! TAATATTGTTTAATGGTACA+TGG - chr2_2:57720997-57721016 Msa0260190:intron 20.0%
! CAGCAACAATAATAATTAGA+TGG + chr2_2:57720348-57720367 None:intergenic 25.0%
!!! TTAAGTTTTTTTATAGCAGG+AGG - chr2_2:57720426-57720445 Msa0260190:intron 25.0%
! GAAAAATTCGATTTACTAAG+TGG + chr2_2:57720806-57720825 None:intergenic 25.0%
!! ACTAAGCAATTAAGCTTTAA+TGG + chr2_2:57720862-57720881 None:intergenic 25.0%
! TGCAGAAACATTGAAATTTA+TGG + chr2_2:57721156-57721175 None:intergenic 25.0%
!! ATATGATAAAAGTGCATAAC+TGG + chr2_2:57721392-57721411 None:intergenic 25.0%
! CAGCAACAATAATAATTAGA+TGG + chr2_2:57720348-57720367 None:intergenic 25.0%
!!! TTAAGTTTTTTTATAGCAGG+AGG - chr2_2:57720426-57720445 Msa0260190:intron 25.0%
! GAAAAATTCGATTTACTAAG+TGG + chr2_2:57720806-57720825 None:intergenic 25.0%
!! ACTAAGCAATTAAGCTTTAA+TGG + chr2_2:57720862-57720881 None:intergenic 25.0%
! TGCAGAAACATTGAAATTTA+TGG + chr2_2:57721156-57721175 None:intergenic 25.0%
!! ATATGATAAAAGTGCATAAC+TGG + chr2_2:57721392-57721411 None:intergenic 25.0%
TTAAGTGTTTGTGTCTAATG+TGG + chr2_2:57719961-57719980 None:intergenic 30.0%
AAACACTTAACACATAAGTG+AGG - chr2_2:57719971-57719990 Msa0260190:three_prime_UTR 30.0%
AGACATCATGTCTAAAAGAA+AGG - chr2_2:57720232-57720251 Msa0260190:CDS 30.0%
AAAAGACAAATTGAACGGTT+TGG - chr2_2:57720502-57720521 Msa0260190:intron 30.0%
! CACATCTATTTCTTAGTGTA+TGG - chr2_2:57720727-57720746 Msa0260190:intron 30.0%
AACAAAGCACACTTAATAAG+TGG - chr2_2:57721124-57721143 Msa0260190:intron 30.0%
CATCTATGAATTATGTCCAT+AGG - chr2_2:57721340-57721359 Msa0260190:five_prime_UTR 30.0%
TTAAGTGTTTGTGTCTAATG+TGG + chr2_2:57719961-57719980 None:intergenic 30.0%
AAACACTTAACACATAAGTG+AGG - chr2_2:57719971-57719990 Msa0260190:three_prime_UTR 30.0%
AGACATCATGTCTAAAAGAA+AGG - chr2_2:57720232-57720251 Msa0260190:CDS 30.0%
AAAAGACAAATTGAACGGTT+TGG - chr2_2:57720502-57720521 Msa0260190:intron 30.0%
! CACATCTATTTCTTAGTGTA+TGG - chr2_2:57720727-57720746 Msa0260190:intron 30.0%
AACAAAGCACACTTAATAAG+TGG - chr2_2:57721124-57721143 Msa0260190:intron 30.0%
CATCTATGAATTATGTCCAT+AGG - chr2_2:57721340-57721359 Msa0260190:five_prime_UTR 30.0%
CATCAATACGTAGAAATGTG+TGG - chr2_2:57720576-57720595 Msa0260190:intron 35.0%
AAATTGTGGTGATTGAAGCA+AGG - chr2_2:57721029-57721048 Msa0260190:intron 35.0%
CATTTGGCTGATACATATAG+AGG - chr2_2:57721061-57721080 Msa0260190:intron 35.0%
AAACTGTTAAGTGTGGAATG+TGG - chr2_2:57721180-57721199 Msa0260190:intron 35.0%
ACGATATTGATGAGTACCTA+TGG + chr2_2:57721359-57721378 None:intergenic 35.0%
CATCAATACGTAGAAATGTG+TGG - chr2_2:57720576-57720595 Msa0260190:intron 35.0%
AAATTGTGGTGATTGAAGCA+AGG - chr2_2:57721029-57721048 Msa0260190:intron 35.0%
CATTTGGCTGATACATATAG+AGG - chr2_2:57721061-57721080 Msa0260190:intron 35.0%
AAACTGTTAAGTGTGGAATG+TGG - chr2_2:57721180-57721199 Msa0260190:intron 35.0%
ACGATATTGATGAGTACCTA+TGG + chr2_2:57721359-57721378 None:intergenic 35.0%
! GACAAATTGAACGGTTTGGA+CGG - chr2_2:57720506-57720525 Msa0260190:intron 40.0%
CATGGATGCAGAAGAAATTG+TGG - chr2_2:57721015-57721034 Msa0260190:intron 40.0%
GCACTCAAAACTGTTAAGTG+TGG - chr2_2:57721173-57721192 Msa0260190:intron 40.0%
! GACAAATTGAACGGTTTGGA+CGG - chr2_2:57720506-57720525 Msa0260190:intron 40.0%
CATGGATGCAGAAGAAATTG+TGG - chr2_2:57721015-57721034 Msa0260190:intron 40.0%
GCACTCAAAACTGTTAAGTG+TGG - chr2_2:57721173-57721192 Msa0260190:intron 40.0%
ATGGAGAAGAGATCAGTAGC+TGG - chr2_2:57720028-57720047 Msa0260190:three_prime_UTR 45.0%
AGCAAGGACTTGTGAGCATT+TGG - chr2_2:57721045-57721064 Msa0260190:intron 45.0%
! AGTTGCATGCATCTTGGTGT+TGG + chr2_2:57721218-57721237 None:intergenic 45.0%
CTTGCAAGTTGCATGCATCT+TGG + chr2_2:57721224-57721243 None:intergenic 45.0%
ATGGAGAAGAGATCAGTAGC+TGG - chr2_2:57720028-57720047 Msa0260190:three_prime_UTR 45.0%
AGCAAGGACTTGTGAGCATT+TGG - chr2_2:57721045-57721064 Msa0260190:intron 45.0%
! AGTTGCATGCATCTTGGTGT+TGG + chr2_2:57721218-57721237 None:intergenic 45.0%
CTTGCAAGTTGCATGCATCT+TGG + chr2_2:57721224-57721243 None:intergenic 45.0%
!! AGTTAATTTATAAAAAAAAA+AGG + chr2_2:57720161-57720180 None:intergenic 5.0%
!!! AGAATATATTTTTTTAAATA+GGG + chr2_2:57720978-57720997 None:intergenic 5.0%
!!! TAGAATATATTTTTTTAAAT+AGG + chr2_2:57720979-57720998 None:intergenic 5.0%
!! AGTTAATTTATAAAAAAAAA+AGG + chr2_2:57720161-57720180 None:intergenic 5.0%
!!! AGAATATATTTTTTTAAATA+GGG + chr2_2:57720978-57720997 None:intergenic 5.0%
!!! TAGAATATATTTTTTTAAAT+AGG + chr2_2:57720979-57720998 None:intergenic 5.0%
CGACAAAGAGAACGAGGAAG+AGG + chr2_2:57720063-57720082 None:intergenic 50.0%
TACGTGCGACAAAGAGAACG+AGG + chr2_2:57720069-57720088 None:intergenic 50.0%
CAGCTAGCATCTGTGAAGCA+TGG + chr2_2:57721088-57721107 None:intergenic 50.0%
CGACAAAGAGAACGAGGAAG+AGG + chr2_2:57720063-57720082 None:intergenic 50.0%
TACGTGCGACAAAGAGAACG+AGG + chr2_2:57720069-57720088 None:intergenic 50.0%
CAGCTAGCATCTGTGAAGCA+TGG + chr2_2:57721088-57721107 None:intergenic 50.0%
! GAACGAGGAAGAGGAAGCAC+AGG + chr2_2:57720054-57720073 None:intergenic 55.0%
! GAACGAGGAAGAGGAAGCAC+AGG + chr2_2:57720054-57720073 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 57719917 57721450 57719917 ID=Msa0260190;Name=Msa0260190
chr2_2 mRNA 57719917 57721450 57719917 ID=Msa0260190-mRNA-1;Parent=Msa0260190;Name=Msa0260190-mRNA-1;_AED=0.36;_eAED=0.36;_QI=111|1|1|1|0|0|2|261|73
chr2_2 exon 57719917 57720341 57719917 ID=Msa0260190-mRNA-1:exon:17618;Parent=Msa0260190-mRNA-1
chr2_2 exon 57721282 57721450 57721282 ID=Msa0260190-mRNA-1:exon:17617;Parent=Msa0260190-mRNA-1
chr2_2 five_prime_UTR 57721340 57721450 57721340 ID=Msa0260190-mRNA-1:five_prime_utr;Parent=Msa0260190-mRNA-1
chr2_2 CDS 57721282 57721339 57721282 ID=Msa0260190-mRNA-1:cds;Parent=Msa0260190-mRNA-1
chr2_2 CDS 57720178 57720341 57720178 ID=Msa0260190-mRNA-1:cds;Parent=Msa0260190-mRNA-1
chr2_2 three_prime_UTR 57719917 57720177 57719917 ID=Msa0260190-mRNA-1:three_prime_utr;Parent=Msa0260190-mRNA-1
Gene Sequence

>Msa0260190

ATGGAGAAGAGATCAGTAGCTGGCCTGTGCTTCCTCTTCCTCGTTCTCTTTGTCGCACAAGAAATTGTGGTGATTGAAGCAAGGACTTGTGAGCATTTGGCTGATACATATAGAGGACCATGCTTCACAGATGCTAGCTGTGACGATCACTGCAAGAACAAAGCACACTTAATAAGTGGCACGTGCCATAAATTTCAATGTTTCTGCACTCAAAACTGTTAA

Protein sequence

>Msa0260190

MEKRSVAGLCFLFLVLFVAQEIVVIEARTCEHLADTYRGPCFTDASCDDHCKNKAHLISGTCHKFQCFCTQNC*