AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1306190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1306190 MtrunA17_Chr2g0317741 89.041 73 8 0 1 73 1 73 1.49e-37 119
Msa1306190 MtrunA17_Chr2g0317751 66.216 74 22 2 1 73 1 72 3.82e-30 101
Msa1306190 MtrunA17_Chr8g0385311 36.000 75 45 2 1 73 1 74 7.03e-12 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 21 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGAAACATTGAAATTTA+TGG 0.161117 8_4:-979250 None:intergenic
ATTATATTCATCACTAAGTA+TGG 0.332590 8_4:+978103 Msa1306190:exon
ATATGATAAAAGTGCATAAC+TGG 0.354165 8_4:-979492 None:intergenic
AGCAAGGACTTGTGAGCATT+TGG 0.402994 8_4:+979142 Msa1306190:CDS
GAACGAGGAAGAGGAAGCAC+AGG 0.435069 8_4:-978145 None:intergenic
CTTGCAAGTTGCATGCATCT+TGG 0.455133 8_4:-979318 None:intergenic
AACAAAGCACACTTAATAAG+TGG 0.478955 8_4:+979221 Msa1306190:CDS
CGACAAAGAGAACGAGGAAG+AGG 0.484363 8_4:-978154 None:intergenic
TTAAGTGTTTGTGTCTAATG+TGG 0.522872 8_4:-978052 None:intergenic
CATTTGGCTGATACATATAG+AGG 0.536909 8_4:+979158 Msa1306190:CDS
AGTTGCATGCATCTTGGTGT+TGG 0.540849 8_4:-979312 None:intergenic
ATGGAGAAGAGATCAGTAGC+TGG 0.575142 8_4:+978122 Msa1306190:CDS
ACGATATTGATGAGTACCTA+TGG 0.577282 8_4:-979459 None:intergenic
CATCTATGAATTATGTCCAT+AGG 0.585201 8_4:+979443 Msa1306190:three_prime_UTR
GCACTCAAAACTGTTAAGTG+TGG 0.610534 8_4:+979270 Msa1306190:exon
AGACACATATATAGCCCCAA+TGG 0.623961 8_4:-978008 None:intergenic
CAGCTAGCATCTGTGAAGCA+TGG 0.627719 8_4:-979182 None:intergenic
CATGGATGCAGAAGAAATTG+TGG 0.636627 8_4:+979112 Msa1306190:intron
AAATTGTGGTGATTGAAGCA+AGG 0.641556 8_4:+979126 Msa1306190:CDS
AAACTGTTAAGTGTGGAATG+TGG 0.677412 8_4:+979277 Msa1306190:exon
TACGTGCGACAAAGAGAACG+AGG 0.732491 8_4:-978160 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTTATAAAAAAAAAAA+AGG - chr8_4:978255-978274 None:intergenic 0.0%
!! TTAATTTATAAAAAAAAAAA+AGG - chr8_4:978255-978274 None:intergenic 0.0%
!! TATATTCTAATATTGTTTAA+TGG + chr8_4:979087-979106 Msa1306190:intron 10.0%
!! TATATTCTAATATTGTTTAA+TGG + chr8_4:979087-979106 Msa1306190:intron 10.0%
!!! TCTTTCTCTAATTTAATTTT+TGG + chr8_4:978390-978409 Msa1306190:intron 15.0%
!! CTTAATTAACAATATACTAA+AGG - chr8_4:978507-978526 None:intergenic 15.0%
!!! AATTAAGTTTTTTTTATAGC+AGG + chr8_4:978520-978539 Msa1306190:intron 15.0%
!! TATATAAATATTACTTTGTC+AGG - chr8_4:978742-978761 None:intergenic 15.0%
!!! TCTTTCTCTAATTTAATTTT+TGG + chr8_4:978390-978409 Msa1306190:intron 15.0%
!! CTTAATTAACAATATACTAA+AGG - chr8_4:978507-978526 None:intergenic 15.0%
!!! AATTAAGTTTTTTTTATAGC+AGG + chr8_4:978520-978539 Msa1306190:intron 15.0%
!! TATATAAATATTACTTTGTC+AGG - chr8_4:978742-978761 None:intergenic 15.0%
!! ATTATATTCATCACTAAGTA+TGG + chr8_4:978103-978122 Msa1306190:exon 20.0%
!! AAAGTATATCTTAAATTTCG+TGG + chr8_4:978293-978312 Msa1306190:intron 20.0%
!! TTAGAGAAAGAAAAAAAAAC+TGG - chr8_4:978381-978400 None:intergenic 20.0%
!! AGCAACAATAATAATTAGAT+GGG - chr8_4:978443-978462 None:intergenic 20.0%
!! ATTAACAATATACTAAAGGA+AGG - chr8_4:978503-978522 None:intergenic 20.0%
!! AAATAAAAAATCGACACATT+TGG - chr8_4:978706-978725 None:intergenic 20.0%
!!! TGTCGATTTTTTATTTGTAA+AGG + chr8_4:978710-978729 Msa1306190:intron 20.0%
!!! ATTTTTTATTTGTAAAGGTC+AGG + chr8_4:978715-978734 Msa1306190:intron 20.0%
!!! ACTAAGCAATTAAGTTTTAA+TGG - chr8_4:978959-978978 None:intergenic 20.0%
!! TAATATTGTTTAATGGTACA+TGG + chr8_4:979094-979113 Msa1306190:intron 20.0%
!! ATTATATTCATCACTAAGTA+TGG + chr8_4:978103-978122 Msa1306190:exon 20.0%
!! AAAGTATATCTTAAATTTCG+TGG + chr8_4:978293-978312 Msa1306190:intron 20.0%
!! TTAGAGAAAGAAAAAAAAAC+TGG - chr8_4:978381-978400 None:intergenic 20.0%
!! AGCAACAATAATAATTAGAT+GGG - chr8_4:978443-978462 None:intergenic 20.0%
!! ATTAACAATATACTAAAGGA+AGG - chr8_4:978503-978522 None:intergenic 20.0%
!! AAATAAAAAATCGACACATT+TGG - chr8_4:978706-978725 None:intergenic 20.0%
!!! TGTCGATTTTTTATTTGTAA+AGG + chr8_4:978710-978729 Msa1306190:intron 20.0%
!!! ATTTTTTATTTGTAAAGGTC+AGG + chr8_4:978715-978734 Msa1306190:intron 20.0%
!!! ACTAAGCAATTAAGTTTTAA+TGG - chr8_4:978959-978978 None:intergenic 20.0%
!! TAATATTGTTTAATGGTACA+TGG + chr8_4:979094-979113 Msa1306190:intron 20.0%
! CAGCAACAATAATAATTAGA+TGG - chr8_4:978444-978463 None:intergenic 25.0%
!!! TAAGTTTTTTTTATAGCAGG+AGG + chr8_4:978523-978542 Msa1306190:intron 25.0%
! GAAAAATTCGATTTACTAAG+TGG - chr8_4:978903-978922 None:intergenic 25.0%
! TGCAGAAACATTGAAATTTA+TGG - chr8_4:979253-979272 None:intergenic 25.0%
!! ATATGATAAAAGTGCATAAC+TGG - chr8_4:979495-979514 None:intergenic 25.0%
! CAGCAACAATAATAATTAGA+TGG - chr8_4:978444-978463 None:intergenic 25.0%
!!! TAAGTTTTTTTTATAGCAGG+AGG + chr8_4:978523-978542 Msa1306190:intron 25.0%
! GAAAAATTCGATTTACTAAG+TGG - chr8_4:978903-978922 None:intergenic 25.0%
! TGCAGAAACATTGAAATTTA+TGG - chr8_4:979253-979272 None:intergenic 25.0%
!! ATATGATAAAAGTGCATAAC+TGG - chr8_4:979495-979514 None:intergenic 25.0%
TTAAGTGTTTGTGTCTAATG+TGG - chr8_4:978055-978074 None:intergenic 30.0%
AGACATCATGTCTAAAAGAA+AGG + chr8_4:978328-978347 Msa1306190:intron 30.0%
AAAAGACAAATTGAACCGTT+TGG + chr8_4:978599-978618 Msa1306190:intron 30.0%
! CACATCTATTTCTTAGTGTA+TGG + chr8_4:978824-978843 Msa1306190:intron 30.0%
AACAAAGCACACTTAATAAG+TGG + chr8_4:979221-979240 Msa1306190:CDS 30.0%
CATCTATGAATTATGTCCAT+AGG + chr8_4:979443-979462 Msa1306190:three_prime_UTR 30.0%
TTAAGTGTTTGTGTCTAATG+TGG - chr8_4:978055-978074 None:intergenic 30.0%
AGACATCATGTCTAAAAGAA+AGG + chr8_4:978328-978347 Msa1306190:intron 30.0%
AAAAGACAAATTGAACCGTT+TGG + chr8_4:978599-978618 Msa1306190:intron 30.0%
! CACATCTATTTCTTAGTGTA+TGG + chr8_4:978824-978843 Msa1306190:intron 30.0%
AACAAAGCACACTTAATAAG+TGG + chr8_4:979221-979240 Msa1306190:CDS 30.0%
CATCTATGAATTATGTCCAT+AGG + chr8_4:979443-979462 Msa1306190:three_prime_UTR 30.0%
CATCAATACGTAGAAATGTG+TGG + chr8_4:978673-978692 Msa1306190:intron 35.0%
AAATTGTGGTGATTGAAGCA+AGG + chr8_4:979126-979145 Msa1306190:CDS 35.0%
CATTTGGCTGATACATATAG+AGG + chr8_4:979158-979177 Msa1306190:CDS 35.0%
AAACTGTTAAGTGTGGAATG+TGG + chr8_4:979277-979296 Msa1306190:exon 35.0%
ACGATATTGATGAGTACCTA+TGG - chr8_4:979462-979481 None:intergenic 35.0%
CATCAATACGTAGAAATGTG+TGG + chr8_4:978673-978692 Msa1306190:intron 35.0%
AAATTGTGGTGATTGAAGCA+AGG + chr8_4:979126-979145 Msa1306190:CDS 35.0%
CATTTGGCTGATACATATAG+AGG + chr8_4:979158-979177 Msa1306190:CDS 35.0%
AAACTGTTAAGTGTGGAATG+TGG + chr8_4:979277-979296 Msa1306190:exon 35.0%
ACGATATTGATGAGTACCTA+TGG - chr8_4:979462-979481 None:intergenic 35.0%
GACAAATTGAACCGTTTGGA+CGG + chr8_4:978603-978622 Msa1306190:intron 40.0%
! TTGTCTGAAATCCGTCCAAA+CGG - chr8_4:978617-978636 None:intergenic 40.0%
CATGGATGCAGAAGAAATTG+TGG + chr8_4:979112-979131 Msa1306190:intron 40.0%
GCACTCAAAACTGTTAAGTG+TGG + chr8_4:979270-979289 Msa1306190:exon 40.0%
GACAAATTGAACCGTTTGGA+CGG + chr8_4:978603-978622 Msa1306190:intron 40.0%
! TTGTCTGAAATCCGTCCAAA+CGG - chr8_4:978617-978636 None:intergenic 40.0%
CATGGATGCAGAAGAAATTG+TGG + chr8_4:979112-979131 Msa1306190:intron 40.0%
GCACTCAAAACTGTTAAGTG+TGG + chr8_4:979270-979289 Msa1306190:exon 40.0%
ATGGAGAAGAGATCAGTAGC+TGG + chr8_4:978122-978141 Msa1306190:CDS 45.0%
AGCAAGGACTTGTGAGCATT+TGG + chr8_4:979142-979161 Msa1306190:CDS 45.0%
! AGTTGCATGCATCTTGGTGT+TGG - chr8_4:979315-979334 None:intergenic 45.0%
CTTGCAAGTTGCATGCATCT+TGG - chr8_4:979321-979340 None:intergenic 45.0%
ATGGAGAAGAGATCAGTAGC+TGG + chr8_4:978122-978141 Msa1306190:CDS 45.0%
AGCAAGGACTTGTGAGCATT+TGG + chr8_4:979142-979161 Msa1306190:CDS 45.0%
! AGTTGCATGCATCTTGGTGT+TGG - chr8_4:979315-979334 None:intergenic 45.0%
CTTGCAAGTTGCATGCATCT+TGG - chr8_4:979321-979340 None:intergenic 45.0%
!!! AGAATATATTTTTTTAAATA+GGG - chr8_4:979075-979094 None:intergenic 5.0%
!!! TAGAATATATTTTTTTAAAT+AGG - chr8_4:979076-979095 None:intergenic 5.0%
!!! AGAATATATTTTTTTAAATA+GGG - chr8_4:979075-979094 None:intergenic 5.0%
!!! TAGAATATATTTTTTTAAAT+AGG - chr8_4:979076-979095 None:intergenic 5.0%
CGACAAAGAGAACGAGGAAG+AGG - chr8_4:978157-978176 None:intergenic 50.0%
TACGTGCGACAAAGAGAACG+AGG - chr8_4:978163-978182 None:intergenic 50.0%
CAGCTAGCATCTGTGAAGCA+TGG - chr8_4:979185-979204 None:intergenic 50.0%
CGACAAAGAGAACGAGGAAG+AGG - chr8_4:978157-978176 None:intergenic 50.0%
TACGTGCGACAAAGAGAACG+AGG - chr8_4:978163-978182 None:intergenic 50.0%
CAGCTAGCATCTGTGAAGCA+TGG - chr8_4:979185-979204 None:intergenic 50.0%
! GAACGAGGAAGAGGAAGCAC+AGG - chr8_4:978148-978167 None:intergenic 55.0%
! GAACGAGGAAGAGGAAGCAC+AGG - chr8_4:978148-978167 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 978011 979553 978011 ID=Msa1306190;Name=Msa1306190
chr8_4 mRNA 978011 979553 978011 ID=Msa1306190-mRNA-1;Parent=Msa1306190;Name=Msa1306190-mRNA-1;_AED=0.25;_eAED=0.25;_QI=111|1|1|1|0|0|2|267|73
chr8_4 exon 978011 978179 978011 ID=Msa1306190-mRNA-1:exon:162;Parent=Msa1306190-mRNA-1
chr8_4 exon 979123 979553 979123 ID=Msa1306190-mRNA-1:exon:163;Parent=Msa1306190-mRNA-1
chr8_4 five_prime_UTR 978011 978121 978011 ID=Msa1306190-mRNA-1:five_prime_utr;Parent=Msa1306190-mRNA-1
chr8_4 CDS 978122 978179 978122 ID=Msa1306190-mRNA-1:cds;Parent=Msa1306190-mRNA-1
chr8_4 CDS 979123 979286 979123 ID=Msa1306190-mRNA-1:cds;Parent=Msa1306190-mRNA-1
chr8_4 three_prime_UTR 979287 979553 979287 ID=Msa1306190-mRNA-1:three_prime_utr;Parent=Msa1306190-mRNA-1
Gene Sequence

>Msa1306190

ATGGAGAAGAGATCAGTAGCTGGCCTGTGCTTCCTCTTCCTCGTTCTCTTTGTCGCACAAGAAATTGTGGTGATTGAAGCAAGGACTTGTGAGCATTTGGCTGATACATATAGAGGACCATGCTTCACAGATGCTAGCTGTGACGATCACTGCAAGAACAAAGCACACTTAATAAGTGGCACGTGCCATAAATTTCAATGTTTCTGCACTCAAAACTGTTAA

Protein sequence

>Msa1306190

MEKRSVAGLCFLFLVLFVAQEIVVIEARTCEHLADTYRGPCFTDASCDDHCKNKAHLISGTCHKFQCFCTQNC*