Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0154200 | sp|Q9FMX6|DJC76_ARATH | 33.990 | 203 | 117 | 5 | 1 | 192 | 263 | 459 | 2.36e-23 | 99.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0154200 | G7IBD4 | 92.268 | 194 | 14 | 1 | 1 | 194 | 267 | 459 | 1.46e-125 | 371 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0014410 | Msa0154200 | 0.882897 | 7.086126e-71 | -8.615850e-47 |
| Msa0035400 | Msa0154200 | 0.801059 | 1.076406e-48 | -8.615850e-47 |
| Msa0063760 | Msa0154200 | 0.882837 | 7.450702e-71 | -8.615850e-47 |
| Msa0064550 | Msa0154200 | 0.830660 | 2.590933e-55 | -8.615850e-47 |
| Msa0108070 | Msa0154200 | 0.816200 | 6.249857e-52 | -8.615850e-47 |
| Msa0109490 | Msa0154200 | 0.827099 | 1.886636e-54 | -8.615850e-47 |
| Msa0154180 | Msa0154200 | 0.943018 | 2.558421e-102 | -8.615850e-47 |
| Msa0154200 | Msa0154220 | 0.896558 | 3.287213e-76 | -8.615850e-47 |
| Msa0154200 | Msa0154240 | 0.828132 | 1.065623e-54 | -8.615850e-47 |
| Msa0154200 | Msa0154270 | 0.884144 | 2.464282e-71 | -8.615850e-47 |
| Msa0154200 | Msa0155610 | 0.823843 | 1.113462e-53 | -8.615850e-47 |
| Msa0154200 | Msa0197030 | 0.802929 | 4.443194e-49 | -8.615850e-47 |
| Msa0154200 | Msa0224370 | 0.806283 | 8.869429e-50 | -8.615850e-47 |
| Msa0154200 | Msa0260190 | 0.826479 | 2.652228e-54 | -8.615850e-47 |
| Msa0154200 | Msa0265580 | 0.813978 | 1.946342e-51 | -8.615850e-47 |
| Msa0154200 | Msa0265980 | -0.809956 | 1.464044e-50 | -8.615850e-47 |
| Msa0154200 | Msa0303440 | 0.818178 | 2.244246e-52 | -8.615850e-47 |
| Msa0154200 | Msa0303500 | 0.817225 | 3.683369e-52 | -8.615850e-47 |
| Msa0154200 | Msa0304110 | 0.803868 | 2.838874e-49 | -8.615850e-47 |
| Msa0154200 | Msa0318070 | 0.800418 | 1.455064e-48 | -8.615850e-47 |
| Msa0154200 | Msa0344680 | 0.824485 | 7.867152e-54 | -8.615850e-47 |
| Msa0154200 | Msa0354390 | 0.830911 | 2.248593e-55 | -8.615850e-47 |
| Msa0154200 | Msa0377280 | 0.808822 | 2.564433e-50 | -8.615850e-47 |
| Msa0154200 | Msa0389060 | 0.801897 | 7.249184e-49 | -8.615850e-47 |
| Msa0154200 | Msa0392050 | 0.825346 | 4.927707e-54 | -8.615850e-47 |
| Msa0154200 | Msa0401340 | 0.829888 | 3.999808e-55 | -8.615850e-47 |
| Msa0154200 | Msa0412190 | 0.803011 | 4.273314e-49 | -8.615850e-47 |
| Msa0154200 | Msa0418430 | 0.814662 | 1.374650e-51 | -8.615850e-47 |
| Msa0154200 | Msa0448120 | 0.830425 | 2.959096e-55 | -8.615850e-47 |
| Msa0154200 | Msa0469950 | 0.826119 | 3.230580e-54 | -8.615850e-47 |
| Msa0154200 | Msa0525270 | 0.803804 | 2.926183e-49 | -8.615850e-47 |
| Msa0154200 | Msa0525280 | 0.800790 | 1.221427e-48 | -8.615850e-47 |
| Msa0154200 | Msa0530950 | 0.811802 | 5.835316e-51 | -8.615850e-47 |
| Msa0154200 | Msa0546080 | 0.804751 | 1.858678e-49 | -8.615850e-47 |
| Msa0154200 | Msa0570680 | 0.805648 | 1.206112e-49 | -8.615850e-47 |
| Msa0154200 | Msa0575890 | 0.801439 | 9.001467e-49 | -8.615850e-47 |
| Msa0154200 | Msa0584280 | 0.827994 | 1.150014e-54 | -8.615850e-47 |
| Msa0154200 | Msa0600820 | 0.803569 | 3.273971e-49 | -8.615850e-47 |
| Msa0154200 | Msa0604880 | 0.800194 | 1.615497e-48 | -8.615850e-47 |
| Msa0154200 | Msa0627050 | 0.825435 | 4.696228e-54 | -8.615850e-47 |
| Msa0154200 | Msa0646190 | 0.829108 | 6.191465e-55 | -8.615850e-47 |
| Msa0154200 | Msa0649310 | 0.811592 | 6.483024e-51 | -8.615850e-47 |
| Msa0154200 | Msa0659300 | -0.804715 | 1.891484e-49 | -8.615850e-47 |
| Msa0154200 | Msa0676100 | 0.810378 | 1.187689e-50 | -8.615850e-47 |
| Msa0154200 | Msa0740210 | 0.811075 | 8.394258e-51 | -8.615850e-47 |
| Msa0154200 | Msa0781390 | 0.807055 | 6.092804e-50 | -8.615850e-47 |
| Msa0154200 | Msa0790640 | 0.805697 | 1.177950e-49 | -8.615850e-47 |
| Msa0154200 | Msa0820520 | 0.808525 | 2.967528e-50 | -8.615850e-47 |
| Msa0154200 | Msa0860920 | 0.814943 | 1.190737e-51 | -8.615850e-47 |
| Msa0154200 | Msa0899700 | 0.820820 | 5.605602e-53 | -8.615850e-47 |
| Msa0154200 | Msa0984000 | 0.833922 | 4.038121e-56 | -8.615850e-47 |
| Msa0154200 | Msa1128650 | -0.810332 | 1.215070e-50 | -8.615850e-47 |
| Msa0154200 | Msa1144730 | 0.825731 | 3.994832e-54 | -8.615850e-47 |
| Msa0154200 | Msa1173380 | 0.804627 | 1.972705e-49 | -8.615850e-47 |
| Msa0154200 | Msa1182830 | 0.806900 | 6.571441e-50 | -8.615850e-47 |
| Msa0154200 | Msa1198720 | 0.804131 | 2.502649e-49 | -8.615850e-47 |
| Msa0154200 | Msa1241820 | 0.814798 | 1.282395e-51 | -8.615850e-47 |
| Msa0154200 | Msa1245120 | -0.821083 | 4.876186e-53 | -8.615850e-47 |
| Msa0154200 | Msa1272950 | 0.817381 | 3.396852e-52 | -8.615850e-47 |
| Msa0154200 | Msa1306190 | 0.812329 | 4.478365e-51 | -8.615850e-47 |
| Msa0154200 | Msa1306200 | 0.811761 | 5.956828e-51 | -8.615850e-47 |
| Msa0154200 | Msa1349810 | 0.821748 | 3.423103e-53 | -8.615850e-47 |
| Msa0154200 | Msa1359890 | 0.812162 | 4.871028e-51 | -8.615850e-47 |
| Msa0154200 | Msa1394900 | 0.805808 | 1.116431e-49 | -8.615850e-47 |
| Msa0154200 | Msa1421870 | 0.811039 | 8.546909e-51 | -8.615850e-47 |
| Msa0154200 | Msa1459540 | 0.811039 | 8.544613e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0154200 | MtrunA17_Chr1g0164721 | 92.268 | 194 | 14 | 1 | 1 | 194 | 285 | 477 | 4.18e-129 | 371 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0154200 | AT5G23240.1 | 33.990 | 203 | 117 | 5 | 1 | 192 | 263 | 459 | 2.40e-24 | 99.4 |
Find 43 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAGGATGAATGGAATTTA+TGG | 0.158588 | 1_4:+18593887 | None:intergenic |
| CAATTTCTAATTGGTTATAT+TGG | 0.226095 | 1_4:-18594049 | Msa0154200:CDS |
| CTCTTTGCAGCTGCATCTCT+TGG | 0.264299 | 1_4:+18593948 | None:intergenic |
| TCTCTTGGCTTACTAAGATT+TGG | 0.268758 | 1_4:+18593963 | None:intergenic |
| CCATTCATCCTGAGGAATAT+TGG | 0.276497 | 1_4:-18593878 | Msa0154200:CDS |
| ACTGGCAATCCCCATCTAAT+TGG | 0.277395 | 1_4:+18593720 | None:intergenic |
| CAATTAGATCAATTTCTAAT+TGG | 0.293169 | 1_4:-18594058 | Msa0154200:CDS |
| CCAGTGGTTACTTCATTGTT+TGG | 0.318448 | 1_4:-18593702 | Msa0154200:CDS |
| TTGGTGCGAATTGCACTATT+TGG | 0.322832 | 1_4:+18594023 | None:intergenic |
| ATGAAAATAGTCCAATTAGA+TGG | 0.341134 | 1_4:-18593731 | Msa0154200:CDS |
| TGGAAATTGTTAACAGCTCT+TGG | 0.381002 | 1_4:-18593602 | Msa0154200:CDS |
| TCAGGATGAATGGAATTTAT+GGG | 0.397759 | 1_4:+18593888 | None:intergenic |
| GAAGGAAGAACAGTAGTTGA+TGG | 0.402925 | 1_4:+18593852 | None:intergenic |
| AGTTGAGTTGAAAGAGCATT+GGG | 0.412413 | 1_4:-18593640 | Msa0154200:CDS |
| TCGATTTCTATTTCCAATGT+TGG | 0.441877 | 1_4:+18593515 | None:intergenic |
| GGTTACTTCATTGTTTGGTA+TGG | 0.442830 | 1_4:-18593697 | Msa0154200:CDS |
| CCAATATTCCTCAGGATGAA+TGG | 0.445444 | 1_4:+18593878 | None:intergenic |
| TTGGGGAGGTTCTCTAGCCT+TGG | 0.460364 | 1_4:-18593622 | Msa0154200:CDS |
| TGAAAATAGTCCAATTAGAT+GGG | 0.463058 | 1_4:-18593730 | Msa0154200:CDS |
| GAAAATGTAGGCTTCTCAAT+TGG | 0.464770 | 1_4:+18593801 | None:intergenic |
| GCAGCTACATGGTATGTGAT+TGG | 0.477504 | 1_4:-18593561 | Msa0154200:CDS |
| AAGGGTTGCTGTAGAATTTG+TGG | 0.483426 | 1_4:-18593538 | Msa0154200:CDS |
| GGCTTACTAAGATTTGGATC+AGG | 0.486859 | 1_4:+18593969 | None:intergenic |
| TGTTTGGTATGGCTGCAGTT+AGG | 0.496837 | 1_4:-18593686 | Msa0154200:CDS |
| CAGTTGAGTTGAAAGAGCAT+TGG | 0.510120 | 1_4:-18593641 | Msa0154200:CDS |
| GTAGTAGTACTAGTTGAAGA+AGG | 0.518499 | 1_4:+18593834 | None:intergenic |
| CTCTTGGTTGCAATATATGT+TGG | 0.521916 | 1_4:-18593586 | Msa0154200:CDS |
| TTCTTCCCTTGAGAAAATGT+AGG | 0.528643 | 1_4:+18593789 | None:intergenic |
| GATGGAGTCCAATATTCCTC+AGG | 0.534720 | 1_4:+18593870 | None:intergenic |
| CTACATGGTATGTGATTGGA+AGG | 0.538431 | 1_4:-18593557 | Msa0154200:CDS |
| GTAGAATTTGTGGCCAACAT+TGG | 0.550242 | 1_4:-18593528 | Msa0154200:CDS |
| CAGGATGAATGGAATTTATG+GGG | 0.557366 | 1_4:+18593889 | None:intergenic |
| ATATGTTGGTAGCAGCTACA+TGG | 0.578806 | 1_4:-18593572 | Msa0154200:CDS |
| AGAGCTGTTAACAATTTCCA+AGG | 0.595776 | 1_4:+18593605 | None:intergenic |
| GCAGTTAGGTTACATGAAGT+AGG | 0.596129 | 1_4:-18593672 | Msa0154200:CDS |
| AATTAGATGGGGATTGCCAG+TGG | 0.600248 | 1_4:-18593718 | Msa0154200:CDS |
| CATAAATTCCATTCATCCTG+AGG | 0.607786 | 1_4:-18593886 | Msa0154200:CDS |
| CCAAACAATGAAGTAACCAC+TGG | 0.612811 | 1_4:+18593702 | None:intergenic |
| AGGATGAATGGAATTTATGG+GGG | 0.638502 | 1_4:+18593890 | None:intergenic |
| GAGTTGAAAGAGCATTGGGG+AGG | 0.639356 | 1_4:-18593636 | Msa0154200:CDS |
| GAAAATAGTCCAATTAGATG+GGG | 0.640775 | 1_4:-18593729 | Msa0154200:CDS |
| GTTGAGTTGAAAGAGCATTG+GGG | 0.697316 | 1_4:-18593639 | Msa0154200:CDS |
| TACATGGTATGTGATTGGAA+GGG | 0.767981 | 1_4:-18593556 | Msa0154200:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAATTAGATCAATTTCTAAT+TGG | - | chr1_4:18593528-18593547 | Msa0154200:CDS | 20.0% |
| !! | CAATTTCTAATTGGTTATAT+TGG | - | chr1_4:18593537-18593556 | Msa0154200:CDS | 20.0% |
| ! | ATGAAAATAGTCCAATTAGA+TGG | - | chr1_4:18593855-18593874 | Msa0154200:CDS | 25.0% |
| ! | TGAAAATAGTCCAATTAGAT+GGG | - | chr1_4:18593856-18593875 | Msa0154200:CDS | 25.0% |
| !! | ATTTATGGGGGTTTTATGTT+TGG | + | chr1_4:18593687-18593706 | None:intergenic | 30.0% |
| TCAGGATGAATGGAATTTAT+GGG | + | chr1_4:18593701-18593720 | None:intergenic | 30.0% | |
| GAAAATAGTCCAATTAGATG+GGG | - | chr1_4:18593857-18593876 | Msa0154200:CDS | 30.0% | |
| TCGATTTCTATTTCCAATGT+TGG | + | chr1_4:18594074-18594093 | None:intergenic | 30.0% | |
| ! | CACAACTTTTGTCATGCTTT+TGG | + | chr1_4:18593585-18593604 | None:intergenic | 35.0% |
| TCTCTTGGCTTACTAAGATT+TGG | + | chr1_4:18593626-18593645 | None:intergenic | 35.0% | |
| AGGATGAATGGAATTTATGG+GGG | + | chr1_4:18593699-18593718 | None:intergenic | 35.0% | |
| CAGGATGAATGGAATTTATG+GGG | + | chr1_4:18593700-18593719 | None:intergenic | 35.0% | |
| CTCAGGATGAATGGAATTTA+TGG | + | chr1_4:18593702-18593721 | None:intergenic | 35.0% | |
| CATAAATTCCATTCATCCTG+AGG | - | chr1_4:18593700-18593719 | Msa0154200:CDS | 35.0% | |
| GTAGTAGTACTAGTTGAAGA+AGG | + | chr1_4:18593755-18593774 | None:intergenic | 35.0% | |
| GAAAATGTAGGCTTCTCAAT+TGG | + | chr1_4:18593788-18593807 | None:intergenic | 35.0% | |
| ! | AGAAGCCTACATTTTCTCAA+GGG | - | chr1_4:18593792-18593811 | Msa0154200:CDS | 35.0% |
| TTCTTCCCTTGAGAAAATGT+AGG | + | chr1_4:18593800-18593819 | None:intergenic | 35.0% | |
| GGTTACTTCATTGTTTGGTA+TGG | - | chr1_4:18593889-18593908 | Msa0154200:CDS | 35.0% | |
| !! | AGTTGAGTTGAAAGAGCATT+GGG | - | chr1_4:18593946-18593965 | Msa0154200:CDS | 35.0% |
| AGAGCTGTTAACAATTTCCA+AGG | + | chr1_4:18593984-18594003 | None:intergenic | 35.0% | |
| TGGAAATTGTTAACAGCTCT+TGG | - | chr1_4:18593984-18594003 | Msa0154200:CDS | 35.0% | |
| CTCTTGGTTGCAATATATGT+TGG | - | chr1_4:18594000-18594019 | Msa0154200:CDS | 35.0% | |
| TACATGGTATGTGATTGGAA+GGG | - | chr1_4:18594030-18594049 | Msa0154200:CDS | 35.0% | |
| TTGGTGCGAATTGCACTATT+TGG | + | chr1_4:18593566-18593585 | None:intergenic | 40.0% | |
| GGCTTACTAAGATTTGGATC+AGG | + | chr1_4:18593620-18593639 | None:intergenic | 40.0% | |
| CCAATATTCCTCAGGATGAA+TGG | + | chr1_4:18593711-18593730 | None:intergenic | 40.0% | |
| CCATTCATCCTGAGGAATAT+TGG | - | chr1_4:18593708-18593727 | Msa0154200:CDS | 40.0% | |
| GAAGGAAGAACAGTAGTTGA+TGG | + | chr1_4:18593737-18593756 | None:intergenic | 40.0% | |
| ! | GAGAAGCCTACATTTTCTCA+AGG | - | chr1_4:18593791-18593810 | Msa0154200:CDS | 40.0% |
| CCAAACAATGAAGTAACCAC+TGG | + | chr1_4:18593887-18593906 | None:intergenic | 40.0% | |
| CCAGTGGTTACTTCATTGTT+TGG | - | chr1_4:18593884-18593903 | Msa0154200:CDS | 40.0% | |
| GCAGTTAGGTTACATGAAGT+AGG | - | chr1_4:18593914-18593933 | Msa0154200:CDS | 40.0% | |
| !! | CAGTTGAGTTGAAAGAGCAT+TGG | - | chr1_4:18593945-18593964 | Msa0154200:CDS | 40.0% |
| !! | GTTGAGTTGAAAGAGCATTG+GGG | - | chr1_4:18593947-18593966 | Msa0154200:CDS | 40.0% |
| !! | ATATGTTGGTAGCAGCTACA+TGG | - | chr1_4:18594014-18594033 | Msa0154200:CDS | 40.0% |
| CTACATGGTATGTGATTGGA+AGG | - | chr1_4:18594029-18594048 | Msa0154200:CDS | 40.0% | |
| AAGGGTTGCTGTAGAATTTG+TGG | - | chr1_4:18594048-18594067 | Msa0154200:CDS | 40.0% | |
| GTAGAATTTGTGGCCAACAT+TGG | - | chr1_4:18594058-18594077 | Msa0154200:CDS | 40.0% | |
| GATGGAGTCCAATATTCCTC+AGG | + | chr1_4:18593719-18593738 | None:intergenic | 45.0% | |
| ACTGGCAATCCCCATCTAAT+TGG | + | chr1_4:18593869-18593888 | None:intergenic | 45.0% | |
| AATTAGATGGGGATTGCCAG+TGG | - | chr1_4:18593868-18593887 | Msa0154200:CDS | 45.0% | |
| TGTTTGGTATGGCTGCAGTT+AGG | - | chr1_4:18593900-18593919 | Msa0154200:CDS | 45.0% | |
| GCAGCTACATGGTATGTGAT+TGG | - | chr1_4:18594025-18594044 | Msa0154200:CDS | 45.0% | |
| CTCTTTGCAGCTGCATCTCT+TGG | + | chr1_4:18593641-18593660 | None:intergenic | 50.0% | |
| !! | GAGTTGAAAGAGCATTGGGG+AGG | - | chr1_4:18593950-18593969 | Msa0154200:CDS | 50.0% |
| TTGGGGAGGTTCTCTAGCCT+TGG | - | chr1_4:18593964-18593983 | Msa0154200:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_4 | gene | 18593512 | 18594096 | 18593512 | ID=Msa0154200;Name=Msa0154200 |
| chr1_4 | mRNA | 18593512 | 18594096 | 18593512 | ID=Msa0154200-mRNA-1;Parent=Msa0154200;Name=Msa0154200-mRNA-1;_AED=0.17;_eAED=0.18;_QI=0|-1|0|1|-1|1|1|0|194 |
| chr1_4 | exon | 18593512 | 18594096 | 18593512 | ID=Msa0154200-mRNA-1:exon:6860;Parent=Msa0154200-mRNA-1 |
| chr1_4 | CDS | 18593512 | 18594096 | 18593512 | ID=Msa0154200-mRNA-1:cds;Parent=Msa0154200-mRNA-1 |
| Gene Sequence |
| Protein sequence |
