AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0154220


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0154220 MtrunA17_Chr1g0164721 92.268 194 14 1 1 194 285 477 4.18e-129 371
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0154220 AT5G23240.1 33.990 203 117 5 1 192 263 459 2.40e-24 99.4

Find 42 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAGGATGAATGGAATTTA+TGG 0.158588 1_4:+18619059 None:intergenic
CAATTTCTAATTGGTTATAT+TGG 0.226095 1_4:-18619221 Msa0154220:CDS
CTCTTTGCAGCTGCATCTCT+TGG 0.264299 1_4:+18619120 None:intergenic
TCTCTTGGCTTACTAAGATT+TGG 0.268758 1_4:+18619135 None:intergenic
CCATTCATCCTGAGGAATAT+TGG 0.276497 1_4:-18619050 Msa0154220:CDS
ACTGGCAATCCCCATCTAAT+TGG 0.277395 1_4:+18618892 None:intergenic
CAATTAGATCAATTTCTAAT+TGG 0.293169 1_4:-18619230 Msa0154220:CDS
CCAGTGGTTACTTCATTGTT+TGG 0.318448 1_4:-18618874 Msa0154220:CDS
TTGGTGCGAATTGCACTATT+TGG 0.322832 1_4:+18619195 None:intergenic
ATGAAAATAGTCCAATTAGA+TGG 0.341134 1_4:-18618903 Msa0154220:CDS
TTGGGGAGGTTCTCTAGCTT+TGG 0.348620 1_4:-18618794 Msa0154220:CDS
TGGAAATTGTTAACAGCTCT+TGG 0.372667 1_4:-18618774 Msa0154220:CDS
TCAGGATGAATGGAATTTAT+GGG 0.397759 1_4:+18619060 None:intergenic
GAAGGAAGAACAGTAGTTGA+TGG 0.402925 1_4:+18619024 None:intergenic
AGTTGAGTTGAAAGAGCATT+GGG 0.412413 1_4:-18618812 Msa0154220:CDS
TCGATTTCTATTTCCAATGT+TGG 0.441877 1_4:+18618687 None:intergenic
GGTTACTTCATTGTTTGGTA+TGG 0.442830 1_4:-18618869 Msa0154220:CDS
CCAATATTCCTCAGGATGAA+TGG 0.445444 1_4:+18619050 None:intergenic
TGAAAATAGTCCAATTAGAT+GGG 0.463058 1_4:-18618902 Msa0154220:CDS
GAAAATGTAGGCTTCTCAAT+TGG 0.464770 1_4:+18618973 None:intergenic
GCAGCTACATGGTATGTGAT+TGG 0.477504 1_4:-18618733 Msa0154220:CDS
AAGGGTTGCTGTAGAATTTG+TGG 0.483426 1_4:-18618710 Msa0154220:CDS
GGCTTACTAAGATTTGGATC+AGG 0.486859 1_4:+18619141 None:intergenic
TGTTTGGTATGGCTGCAGTT+AGG 0.496837 1_4:-18618858 Msa0154220:CDS
CAGTTGAGTTGAAAGAGCAT+TGG 0.510120 1_4:-18618813 Msa0154220:CDS
GTAGTAGTACTAGTTGAAGA+AGG 0.518499 1_4:+18619006 None:intergenic
CTCTTGGTTGCAATATATGT+TGG 0.521916 1_4:-18618758 Msa0154220:CDS
TTCTTCCCTTGAGAAAATGT+AGG 0.528643 1_4:+18618961 None:intergenic
GATGGAGTCCAATATTCCTC+AGG 0.534720 1_4:+18619042 None:intergenic
CTACATGGTATGTGATTGGA+AGG 0.538431 1_4:-18618729 Msa0154220:CDS
GTAGAATTTGTGGCCAACAT+TGG 0.550242 1_4:-18618700 Msa0154220:CDS
CAGGATGAATGGAATTTATG+GGG 0.557366 1_4:+18619061 None:intergenic
ATATGTTGGTAGCAGCTACA+TGG 0.578806 1_4:-18618744 Msa0154220:CDS
GCAGTTAGGTTACATGAAGT+AGG 0.596129 1_4:-18618844 Msa0154220:CDS
AATTAGATGGGGATTGCCAG+TGG 0.600248 1_4:-18618890 Msa0154220:CDS
CATAAATTCCATTCATCCTG+AGG 0.607786 1_4:-18619058 Msa0154220:CDS
CCAAACAATGAAGTAACCAC+TGG 0.612811 1_4:+18618874 None:intergenic
AGGATGAATGGAATTTATGG+GGG 0.638502 1_4:+18619062 None:intergenic
GAGTTGAAAGAGCATTGGGG+AGG 0.639356 1_4:-18618808 Msa0154220:CDS
GAAAATAGTCCAATTAGATG+GGG 0.640775 1_4:-18618901 Msa0154220:CDS
GTTGAGTTGAAAGAGCATTG+GGG 0.697316 1_4:-18618811 Msa0154220:CDS
TACATGGTATGTGATTGGAA+GGG 0.767981 1_4:-18618728 Msa0154220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTAGATCAATTTCTAAT+TGG - chr1_4:18618700-18618719 Msa0154220:CDS 20.0%
!! CAATTTCTAATTGGTTATAT+TGG - chr1_4:18618709-18618728 Msa0154220:CDS 20.0%
! ATGAAAATAGTCCAATTAGA+TGG - chr1_4:18619027-18619046 Msa0154220:CDS 25.0%
! TGAAAATAGTCCAATTAGAT+GGG - chr1_4:18619028-18619047 Msa0154220:CDS 25.0%
!! ATTTATGGGGGTTTTATGTT+TGG + chr1_4:18618859-18618878 None:intergenic 30.0%
TCAGGATGAATGGAATTTAT+GGG + chr1_4:18618873-18618892 None:intergenic 30.0%
GAAAATAGTCCAATTAGATG+GGG - chr1_4:18619029-18619048 Msa0154220:CDS 30.0%
TCGATTTCTATTTCCAATGT+TGG + chr1_4:18619246-18619265 None:intergenic 30.0%
! CACAACTTTTGTCATGCTTT+TGG + chr1_4:18618757-18618776 None:intergenic 35.0%
TCTCTTGGCTTACTAAGATT+TGG + chr1_4:18618798-18618817 None:intergenic 35.0%
AGGATGAATGGAATTTATGG+GGG + chr1_4:18618871-18618890 None:intergenic 35.0%
CAGGATGAATGGAATTTATG+GGG + chr1_4:18618872-18618891 None:intergenic 35.0%
CTCAGGATGAATGGAATTTA+TGG + chr1_4:18618874-18618893 None:intergenic 35.0%
CATAAATTCCATTCATCCTG+AGG - chr1_4:18618872-18618891 Msa0154220:CDS 35.0%
GTAGTAGTACTAGTTGAAGA+AGG + chr1_4:18618927-18618946 None:intergenic 35.0%
GAAAATGTAGGCTTCTCAAT+TGG + chr1_4:18618960-18618979 None:intergenic 35.0%
! AGAAGCCTACATTTTCTCAA+GGG - chr1_4:18618964-18618983 Msa0154220:CDS 35.0%
TTCTTCCCTTGAGAAAATGT+AGG + chr1_4:18618972-18618991 None:intergenic 35.0%
GGTTACTTCATTGTTTGGTA+TGG - chr1_4:18619061-18619080 Msa0154220:CDS 35.0%
!! AGTTGAGTTGAAAGAGCATT+GGG - chr1_4:18619118-18619137 Msa0154220:CDS 35.0%
TGGAAATTGTTAACAGCTCT+TGG - chr1_4:18619156-18619175 Msa0154220:CDS 35.0%
CTCTTGGTTGCAATATATGT+TGG - chr1_4:18619172-18619191 Msa0154220:CDS 35.0%
TACATGGTATGTGATTGGAA+GGG - chr1_4:18619202-18619221 Msa0154220:CDS 35.0%
TTGGTGCGAATTGCACTATT+TGG + chr1_4:18618738-18618757 None:intergenic 40.0%
GGCTTACTAAGATTTGGATC+AGG + chr1_4:18618792-18618811 None:intergenic 40.0%
CCAATATTCCTCAGGATGAA+TGG + chr1_4:18618883-18618902 None:intergenic 40.0%
CCATTCATCCTGAGGAATAT+TGG - chr1_4:18618880-18618899 Msa0154220:CDS 40.0%
GAAGGAAGAACAGTAGTTGA+TGG + chr1_4:18618909-18618928 None:intergenic 40.0%
! GAGAAGCCTACATTTTCTCA+AGG - chr1_4:18618963-18618982 Msa0154220:CDS 40.0%
CCAAACAATGAAGTAACCAC+TGG + chr1_4:18619059-18619078 None:intergenic 40.0%
CCAGTGGTTACTTCATTGTT+TGG - chr1_4:18619056-18619075 Msa0154220:CDS 40.0%
GCAGTTAGGTTACATGAAGT+AGG - chr1_4:18619086-18619105 Msa0154220:CDS 40.0%
!! CAGTTGAGTTGAAAGAGCAT+TGG - chr1_4:18619117-18619136 Msa0154220:CDS 40.0%
!! GTTGAGTTGAAAGAGCATTG+GGG - chr1_4:18619119-18619138 Msa0154220:CDS 40.0%
!! ATATGTTGGTAGCAGCTACA+TGG - chr1_4:18619186-18619205 Msa0154220:CDS 40.0%
CTACATGGTATGTGATTGGA+AGG - chr1_4:18619201-18619220 Msa0154220:CDS 40.0%
AAGGGTTGCTGTAGAATTTG+TGG - chr1_4:18619220-18619239 Msa0154220:CDS 40.0%
GTAGAATTTGTGGCCAACAT+TGG - chr1_4:18619230-18619249 Msa0154220:CDS 40.0%
GATGGAGTCCAATATTCCTC+AGG + chr1_4:18618891-18618910 None:intergenic 45.0%
ACTGGCAATCCCCATCTAAT+TGG + chr1_4:18619041-18619060 None:intergenic 45.0%
AATTAGATGGGGATTGCCAG+TGG - chr1_4:18619040-18619059 Msa0154220:CDS 45.0%
TGTTTGGTATGGCTGCAGTT+AGG - chr1_4:18619072-18619091 Msa0154220:CDS 45.0%
GCAGCTACATGGTATGTGAT+TGG - chr1_4:18619197-18619216 Msa0154220:CDS 45.0%
CTCTTTGCAGCTGCATCTCT+TGG + chr1_4:18618813-18618832 None:intergenic 50.0%
!! GAGTTGAAAGAGCATTGGGG+AGG - chr1_4:18619122-18619141 Msa0154220:CDS 50.0%
! TTGGGGAGGTTCTCTAGCTT+TGG - chr1_4:18619136-18619155 Msa0154220:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 18618684 18619268 18618684 ID=Msa0154220;Name=Msa0154220
chr1_4 mRNA 18618684 18619268 18618684 ID=Msa0154220-mRNA-1;Parent=Msa0154220;Name=Msa0154220-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0|-1|0|1|-1|1|1|0|194
chr1_4 exon 18618684 18619268 18618684 ID=Msa0154220-mRNA-1:exon:6861;Parent=Msa0154220-mRNA-1
chr1_4 CDS 18618684 18619268 18618684 ID=Msa0154220-mRNA-1:cds;Parent=Msa0154220-mRNA-1
Gene Sequence

>Msa0154220

ATGTCAGCAATTCAAGCAATTAGATCAATTTCTAATTGGTTATATTGGCAACCAAATAGTGCAATTCGCACCAAAAGCATGACAAAAGTTGTGCAAAAATTACTACCTGATCCAAATCTTAGTAAGCCAAGAGATGCAGCTGCAAAGAGAAAATTAAGAGATACACAAAAAACCAAACATAAAACCCCCATAAATTCCATTCATCCTGAGGAATATTGGACTCCATCAACTACTGTTCTTCCTTCTTCAACTAGTACTACTACTACTACTACACCAATTGAGAAGCCTACATTTTCTCAAGGGAAGAAACAAAGAAAAAGAAGTGATATTGAAACATATGAGAATGAAAATAGTCCAATTAGATGGGGATTGCCAGTGGTTACTTCATTGTTTGGTATGGCTGCAGTTAGGTTACATGAAGTAGGATCATCAACAGTTGAGTTGAAAGAGCATTGGGGAGGTTCTCTAGCTTTGGAAATTGTTAACAGCTCTTGGTTGCAATATATGTTGGTAGCAGCTACATGGTATGTGATTGGAAGGGTTGCTGTAGAATTTGTGGCCAACATTGGAAATAGAAATCGATAG

Protein sequence

>Msa0154220

MSAIQAIRSISNWLYWQPNSAIRTKSMTKVVQKLLPDPNLSKPRDAAAKRKLRDTQKTKHKTPINSIHPEEYWTPSTTVLPSSTSTTTTTTPIEKPTFSQGKKQRKRSDIETYENENSPIRWGLPVVTSLFGMAAVRLHEVGSSTVELKEHWGGSLALEIVNSSWLQYMLVAATWYVIGRVAVEFVANIGNRNR*