AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0469820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0469820 MtrunA17_Chr3g0124651 99.548 221 0 1 1 221 1 220 9.73e-163 447
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0469820 AT1G21830.1 41.228 228 94 10 4 218 2 202 1.14e-35 125
Msa0469820 AT1G44608.1 35.263 190 87 7 13 199 12 168 1.39e-18 80.1

Find 95 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATTATTTGTTTGTGTCTT+TGG 0.113742 3_3:+71821313 Msa0469820:three_prime_UTR
TTTGCATGCTTTGAGTATTT+TGG 0.155308 3_3:+71821536 Msa0469820:three_prime_UTR
GAAAGTGACACCAATTAATT+TGG 0.199806 3_3:+71821258 Msa0469820:three_prime_UTR
AATTCCATAACCAAATTAAT+TGG 0.200699 3_3:-71821268 None:intergenic
ACCTAACATGGGGAGTATTT+TGG 0.214727 3_3:-71821732 None:intergenic
AAGACAGTACAGGCTGTAAA+AGG 0.240073 3_3:+71821623 Msa0469820:three_prime_UTR
CTATTCATGTTTGTTAATAA+AGG 0.249204 3_3:-71819207 None:intergenic
ATCCTTCACACTTCACAGTA+TGG 0.272053 3_3:-71821699 None:intergenic
GTGATGTGAACTAATTTAAA+TGG 0.285211 3_3:-71819095 None:intergenic
ACGCCAACAACTACTTGTTT+AGG 0.289509 3_3:-71819270 None:intergenic
ATTCATAAGATCTTTGCTTT+TGG 0.295772 3_3:+71819459 Msa0469820:CDS
CGCCAACAACTACTTGTTTA+GGG 0.296896 3_3:-71819269 None:intergenic
AAGAAAGTGAATTGTGAAAA+AGG 0.296918 3_3:-71819160 None:intergenic
GACACCAATTAATTTGGTTA+TGG 0.306860 3_3:+71821264 Msa0469820:three_prime_UTR
TCAATCAAGTTTGAAGGAAA+TGG 0.311026 3_3:+71820932 Msa0469820:CDS
GAATGAAAGGCTTCTTCTAT+TGG 0.318441 3_3:+71819308 Msa0469820:CDS
CTTTATTAACAAACATGAAT+AGG 0.318817 3_3:+71819208 Msa0469820:exon
CAAAGTCACGTGACACATTC+AGG 0.325911 3_3:+71821062 Msa0469820:CDS
AGCCTTTCATTCAAGCATAA+AGG 0.335003 3_3:-71819297 None:intergenic
TTATGAATAGAAGAAGAATT+TGG 0.340183 3_3:-71819444 None:intergenic
ACATGAATAGGAATGGATAT+AGG 0.360762 3_3:+71819220 Msa0469820:exon
CAATCAAGTTTGAAGGAAAT+GGG 0.363983 3_3:+71820933 Msa0469820:CDS
GAAACTAAAATAGAAGAGTA+TGG 0.367581 3_3:-71819025 None:intergenic
GCCTTTCATTCAAGCATAAA+GGG 0.367664 3_3:-71819296 None:intergenic
CTCATAAGGTGGAAGAAAAC+TGG 0.371691 3_3:+71821115 Msa0469820:CDS
CATGAATAGGAATGGATATA+GGG 0.372518 3_3:+71819221 Msa0469820:exon
GTCCTGTAGCATTATACAAT+AGG 0.378100 3_3:-71821788 None:intergenic
GGTTCTTCTTGCAATTCTCT+AGG 0.383703 3_3:-71821000 None:intergenic
TGGTCTCTAATTTGTCCTCA+TGG 0.391766 3_3:-71821021 None:intergenic
ATTATTGAGTATATATATGT+TGG 0.391855 3_3:-71819056 None:intergenic
CTTCATTGTTTCCCACTACA+TGG 0.398038 3_3:-71821755 None:intergenic
CACCCTAAACAAGTAGTTGT+TGG 0.402840 3_3:+71819267 Msa0469820:CDS
TTCATATCAATCAAGTTTGA+AGG 0.415927 3_3:+71820926 Msa0469820:CDS
GCCCTTTATGCTTGAATGAA+AGG 0.420777 3_3:+71819295 Msa0469820:CDS
GCCAAAATACTCCCCATGTT+AGG 0.451646 3_3:+71821731 Msa0469820:three_prime_UTR
GTCACATGTTCCAGCTCATA+AGG 0.455731 3_3:+71821101 Msa0469820:CDS
GTGGGAAACAATGAAGCAAA+AGG 0.456814 3_3:+71821762 Msa0469820:three_prime_UTR
TGCATGCTCTATCACACTCT+TGG 0.456919 3_3:-71821041 None:intergenic
CTACTATTCATATTGACATA+TGG 0.459031 3_3:+71821383 Msa0469820:three_prime_UTR
TCCTGTAGCATTATACAATA+GGG 0.460383 3_3:-71821787 None:intergenic
GTTCTTCTTGCAATTCTCTA+GGG 0.470567 3_3:-71820999 None:intergenic
CCCACTACATGGACCTAACA+TGG 0.475730 3_3:-71821744 None:intergenic
AAGAAGAACGGTGGTTATGA+TGG 0.478238 3_3:-71819353 None:intergenic
TTGTCTTATAGTTGTCTTAG+TGG 0.478336 3_3:+71821811 Msa0469820:three_prime_UTR
TTATTGAGTATATATATGTT+GGG 0.485571 3_3:-71819055 None:intergenic
CACGTAGGCAACAAGGTTGA+AGG 0.495184 3_3:+71821148 Msa0469820:CDS
TCCCTATTGTATAATGCTAC+AGG 0.496181 3_3:+71821786 Msa0469820:three_prime_UTR
TGAATAGAAGAAGAATTTGG+TGG 0.500634 3_3:-71819441 None:intergenic
GTCTACTATACTTTATTCCA+TGG 0.505676 3_3:-71821230 None:intergenic
TTAACAAACATGAATAGGAA+TGG 0.508320 3_3:+71819213 Msa0469820:exon
CTTCTATTGGCTGCAAAACA+AGG 0.509299 3_3:+71819321 Msa0469820:CDS
TGTCTTATAGTTGTCTTAGT+GGG 0.521254 3_3:+71821812 Msa0469820:three_prime_UTR
TCCTTCACACTTCACAGTAT+GGG 0.522668 3_3:-71821698 None:intergenic
AACTAATTTAAATGGATGGA+TGG 0.530223 3_3:-71819087 None:intergenic
ATTTGGTGGCTTTGTGTGTA+CGG 0.533418 3_3:-71819427 None:intergenic
CTTCTTGCAATTCTCTAGGG+AGG 0.538057 3_3:-71820996 None:intergenic
AATTTAAATGGATGGATGGA+AGG 0.543920 3_3:-71819083 None:intergenic
AAACTAAAATAGAAGAGTAT+GGG 0.548003 3_3:-71819024 None:intergenic
ACACATTCAGGTGGAGAAAG+AGG 0.548112 3_3:+71821074 Msa0469820:CDS
TGTGAACTAATTTAAATGGA+TGG 0.550623 3_3:-71819091 None:intergenic
AGACAGTACAGGCTGTAAAA+GGG 0.553990 3_3:+71821624 Msa0469820:three_prime_UTR
CAGTGAAAGTCAGAAAAGGC+TGG 0.555530 3_3:+71821176 Msa0469820:CDS
GGAGCAGTGAAAGTCAGAAA+AGG 0.558648 3_3:+71821172 Msa0469820:CDS
CTTGCAATTCTCTAGGGAGG+AGG 0.560179 3_3:-71820993 None:intergenic
CCCATGTTAGGTCCATGTAG+TGG 0.563434 3_3:+71821743 Msa0469820:three_prime_UTR
TCTCGTCACTGCAAATCTCA+TGG 0.576256 3_3:+71819504 Msa0469820:CDS
CTTCAACCTTGTTGCCTACG+TGG 0.576338 3_3:-71821147 None:intergenic
AAGTCAGAAAAGGCTGGATG+AGG 0.577043 3_3:+71821182 Msa0469820:CDS
TAGAAGATGAAGAAGAAGAA+CGG 0.577088 3_3:-71819365 None:intergenic
TTCAGGTGGAGAAAGAGGAT+TGG 0.577306 3_3:+71821079 Msa0469820:CDS
CCATGTTAGGTCCATGTAGT+GGG 0.578380 3_3:+71821744 Msa0469820:three_prime_UTR
GATATCTCTCCTAGTCCAGA+AGG 0.583190 3_3:+71819543 Msa0469820:CDS
TGGGAAACAATGAAGCAAAA+GGG 0.584762 3_3:+71821763 Msa0469820:three_prime_UTR
GGAAAAGAGCACGGTCTCAA+AGG 0.587379 3_3:+71820967 Msa0469820:CDS
TAAATAGCAAAGTGATGATG+AGG 0.597282 3_3:-71821337 None:intergenic
AGGAAATGGGGTAGCCTCGT+GGG 0.614477 3_3:+71820946 Msa0469820:CDS
TATTGGCTGCAAAACAAGGT+CGG 0.618913 3_3:+71819325 Msa0469820:CDS
CCACTACATGGACCTAACAT+GGG 0.623852 3_3:-71821743 None:intergenic
TGTATGCCACGTAGGCAACA+AGG 0.624880 3_3:+71821141 Msa0469820:CDS
AAGGAAATGGGGTAGCCTCG+TGG 0.628547 3_3:+71820945 Msa0469820:CDS
ACATGTTCCAGCTCATAAGG+TGG 0.637291 3_3:+71821104 Msa0469820:CDS
TCCCATACTGTGAAGTGTGA+AGG 0.637445 3_3:+71821697 Msa0469820:three_prime_UTR
AATCAAGTTTGAAGGAAATG+GGG 0.642471 3_3:+71820934 Msa0469820:CDS
AGCCTCGTGGGAAAAGAGCA+CGG 0.642699 3_3:+71820958 Msa0469820:CDS
AAGAACGGTGGTTATGATGG+TGG 0.643533 3_3:-71819350 None:intergenic
AAAGAGAAAGAAGAAGACCA+TGG 0.647060 3_3:+71821213 Msa0469820:CDS
CAACAACTACTTGTTTAGGG+TGG 0.653474 3_3:-71819266 None:intergenic
AAGATGAAGAAGAAGAACGG+TGG 0.658130 3_3:-71819362 None:intergenic
CACTACATGGACCTAACATG+GGG 0.665065 3_3:-71821742 None:intergenic
GTAGGCAACAAGGTTGAAGG+AGG 0.670514 3_3:+71821151 Msa0469820:CDS
ACTGGAGGTGTATGCCACGT+AGG 0.690562 3_3:+71821133 Msa0469820:CDS
TTAGGGTGGAAATTGCAACA+TGG 0.705580 3_3:-71819252 None:intergenic
ATAAGGTGGAAGAAAACTGG+AGG 0.709203 3_3:+71821118 Msa0469820:CDS
AGTCACGTGACACATTCAGG+TGG 0.713866 3_3:+71821065 Msa0469820:CDS
GAATTGCAAGAAGAACCATG+AGG 0.821093 3_3:+71821006 Msa0469820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTATATTTCAAGATTA+TGG + chr3_3:71820214-71820233 Msa0469820:intron 10.0%
!! TTATACTTTATACAATATTA+GGG - chr3_3:71820674-71820693 None:intergenic 10.0%
!! TGTTAGTAATTAAATTAATT+TGG - chr3_3:71820719-71820738 None:intergenic 10.0%
!! TTATTGAGTATATATATGTT+GGG - chr3_3:71819058-71819077 None:intergenic 15.0%
!! ATTATTGAGTATATATATGT+TGG - chr3_3:71819059-71819078 None:intergenic 15.0%
!!! ATTTGGAAAAAAAATCTTTT+GGG - chr3_3:71819839-71819858 None:intergenic 15.0%
!!! AATTTGGAAAAAAAATCTTT+TGG - chr3_3:71819840-71819859 None:intergenic 15.0%
!!! CAACTTCTTTTTAATTAATT+TGG - chr3_3:71819856-71819875 None:intergenic 15.0%
!! CAAATTAATTAAAAAGAAGT+TGG + chr3_3:71819854-71819873 Msa0469820:intron 15.0%
!! CAATAATGAAAAGAAATTAA+TGG - chr3_3:71819975-71819994 None:intergenic 15.0%
!!! CATTAATTTCTTTTCATTAT+TGG + chr3_3:71819973-71819992 Msa0469820:intron 15.0%
!! ATAATAATAATGCTCTTAAT+TGG - chr3_3:71820186-71820205 None:intergenic 15.0%
!! GTTATACTTTATACAATATT+AGG - chr3_3:71820675-71820694 None:intergenic 15.0%
!!! TTTTTGTAATTTTCCTTTTT+AGG + chr3_3:71821571-71821590 Msa0469820:three_prime_UTR 15.0%
!!! TTTTGTAATTTTCCTTTTTA+GGG + chr3_3:71821572-71821591 Msa0469820:three_prime_UTR 15.0%
!!! AAACTAAAATAGAAGAGTAT+GGG - chr3_3:71819027-71819046 None:intergenic 20.0%
!!! GTTTGTTAATAAAGGTTTTT+TGG - chr3_3:71819202-71819221 None:intergenic 20.0%
!!! CTATTCATGTTTGTTAATAA+AGG - chr3_3:71819210-71819229 None:intergenic 20.0%
!! CTTTATTAACAAACATGAAT+AGG + chr3_3:71819208-71819227 Msa0469820:exon 20.0%
!! TTATGAATAGAAGAAGAATT+TGG - chr3_3:71819447-71819466 None:intergenic 20.0%
!!! ATATTAATGATTTTACCTTC+TGG - chr3_3:71819561-71819580 None:intergenic 20.0%
!! ATTAATTAAAAAGAAGTTGG+TGG + chr3_3:71819857-71819876 Msa0469820:intron 20.0%
!!! ATTAGTTGTTGATATTTTCA+TGG + chr3_3:71819925-71819944 Msa0469820:intron 20.0%
!!! TAATTTCTTTTCATTATTGG+AGG + chr3_3:71819976-71819995 Msa0469820:intron 20.0%
!!! CATTGAACTATTTTTAATAG+TGG - chr3_3:71820321-71820340 None:intergenic 20.0%
!! AATTCCATAACCAAATTAAT+TGG - chr3_3:71821271-71821290 None:intergenic 20.0%
!!! CTTTTTCTTTTTCCATTTTT+TGG - chr3_3:71821496-71821515 None:intergenic 20.0%
!! AATGGAAAAAGAAAAAGAAA+AGG + chr3_3:71821499-71821518 Msa0469820:three_prime_UTR 20.0%
!! ATGGAAAAAGAAAAAGAAAA+GGG + chr3_3:71821500-71821519 Msa0469820:three_prime_UTR 20.0%
!! TGAAAATAAAAACCCTAAAA+AGG - chr3_3:71821587-71821606 None:intergenic 20.0%
! GAAACTAAAATAGAAGAGTA+TGG - chr3_3:71819028-71819047 None:intergenic 25.0%
! AACTAATTTAAATGGATGGA+TGG - chr3_3:71819090-71819109 None:intergenic 25.0%
! TGTGAACTAATTTAAATGGA+TGG - chr3_3:71819094-71819113 None:intergenic 25.0%
! GTGATGTGAACTAATTTAAA+TGG - chr3_3:71819098-71819117 None:intergenic 25.0%
! AAAAAGGAAAGAAAGAAGAA+GGG - chr3_3:71819147-71819166 None:intergenic 25.0%
! AAGAAAGTGAATTGTGAAAA+AGG - chr3_3:71819163-71819182 None:intergenic 25.0%
! TTAACAAACATGAATAGGAA+TGG + chr3_3:71819213-71819232 Msa0469820:exon 25.0%
!! ATTCATAAGATCTTTGCTTT+TGG + chr3_3:71819459-71819478 Msa0469820:CDS 25.0%
! AAAGATTAATCTTTCGAATC+AGG - chr3_3:71819787-71819806 None:intergenic 25.0%
! ATTCGAAAGATTAATCTTTG+CGG + chr3_3:71819788-71819807 Msa0469820:intron 25.0%
!! TTGTTGATATTTTCATGGTT+TGG + chr3_3:71819930-71819949 Msa0469820:intron 25.0%
! TATTTCAAGATTATGGTATG+TGG + chr3_3:71820221-71820240 Msa0469820:intron 25.0%
! ACTAGTAAATCAAATGAGAT+AGG + chr3_3:71820402-71820421 Msa0469820:intron 25.0%
!!! CTTGTCTATGTATTTTTACT+TGG + chr3_3:71820815-71820834 Msa0469820:intron 25.0%
! TTCATATCAATCAAGTTTGA+AGG + chr3_3:71820926-71820945 Msa0469820:CDS 25.0%
! CTATTATTTGTTTGTGTCTT+TGG + chr3_3:71821313-71821332 Msa0469820:three_prime_UTR 25.0%
! CTACTATTCATATTGACATA+TGG + chr3_3:71821383-71821402 Msa0469820:three_prime_UTR 25.0%
!! TGCTTTAGATTACCAAAAAA+TGG + chr3_3:71821481-71821500 Msa0469820:three_prime_UTR 25.0%
AATTTAAATGGATGGATGGA+AGG - chr3_3:71819086-71819105 None:intergenic 30.0%
GAAAAAGGAAAGAAAGAAGA+AGG - chr3_3:71819148-71819167 None:intergenic 30.0%
ACATGAATAGGAATGGATAT+AGG + chr3_3:71819220-71819239 Msa0469820:exon 30.0%
CATGAATAGGAATGGATATA+GGG + chr3_3:71819221-71819240 Msa0469820:exon 30.0%
TAGAAGATGAAGAAGAAGAA+CGG - chr3_3:71819368-71819387 None:intergenic 30.0%
!!! TTGTGAACTAGCAACTTTTT+TGG - chr3_3:71819394-71819413 None:intergenic 30.0%
TGAATAGAAGAAGAATTTGG+TGG - chr3_3:71819444-71819463 None:intergenic 30.0%
!! AAAAAGAAGTTGGTGGAAAA+AGG + chr3_3:71819864-71819883 Msa0469820:intron 30.0%
!!! TGATATTTTCATGGTTTGGT+TGG + chr3_3:71819934-71819953 Msa0469820:intron 30.0%
!!! GATATTTTCATGGTTTGGTT+GGG + chr3_3:71819935-71819954 Msa0469820:intron 30.0%
!!! ATATTTTCATGGTTTGGTTG+GGG + chr3_3:71819936-71819955 Msa0469820:intron 30.0%
! TTGTGATGATGACGAAATTA+AGG + chr3_3:71820052-71820071 Msa0469820:intron 30.0%
ACTAGTAGTAGTAAGACATA+AGG + chr3_3:71820089-71820108 Msa0469820:intron 30.0%
TAGTAGTAAGACATAAGGAT+GGG + chr3_3:71820094-71820113 Msa0469820:intron 30.0%
GAGCATTATTATTATGACAC+AGG + chr3_3:71820191-71820210 Msa0469820:intron 30.0%
AAGATTATGGTATGTGGATT+TGG + chr3_3:71820227-71820246 Msa0469820:intron 30.0%
ATTAGGGTACACAACTAATT+TGG + chr3_3:71820256-71820275 Msa0469820:intron 30.0%
TCAAATTGTATCAAATCCGT+CGG + chr3_3:71820596-71820615 Msa0469820:intron 30.0%
AGTAGCTGAGCATAAAAATT+AGG + chr3_3:71820631-71820650 Msa0469820:intron 30.0%
! TCATTAGATTTTGCTTTGTG+TGG + chr3_3:71820843-71820862 Msa0469820:intron 30.0%
! CATTAGATTTTGCTTTGTGT+GGG + chr3_3:71820844-71820863 Msa0469820:intron 30.0%
ATTCTCATTTCTCATTCCAA+GGG - chr3_3:71820876-71820895 None:intergenic 30.0%
TCAATCAAGTTTGAAGGAAA+TGG + chr3_3:71820932-71820951 Msa0469820:CDS 30.0%
CAATCAAGTTTGAAGGAAAT+GGG + chr3_3:71820933-71820952 Msa0469820:CDS 30.0%
AATCAAGTTTGAAGGAAATG+GGG + chr3_3:71820934-71820953 Msa0469820:CDS 30.0%
GTCTACTATACTTTATTCCA+TGG - chr3_3:71821233-71821252 None:intergenic 30.0%
GAAAGTGACACCAATTAATT+TGG + chr3_3:71821258-71821277 Msa0469820:three_prime_UTR 30.0%
! GACACCAATTAATTTGGTTA+TGG + chr3_3:71821264-71821283 Msa0469820:three_prime_UTR 30.0%
! TAAATAGCAAAGTGATGATG+AGG - chr3_3:71821340-71821359 None:intergenic 30.0%
!! TTTGCATGCTTTGAGTATTT+TGG + chr3_3:71821536-71821555 Msa0469820:three_prime_UTR 30.0%
!! TTGCATGCTTTGAGTATTTT+GGG + chr3_3:71821537-71821556 Msa0469820:three_prime_UTR 30.0%
CTTCATAAAAAAGACAGTAC+AGG + chr3_3:71821613-71821632 Msa0469820:three_prime_UTR 30.0%
TCCTGTAGCATTATACAATA+GGG - chr3_3:71821790-71821809 None:intergenic 30.0%
TTGTCTTATAGTTGTCTTAG+TGG + chr3_3:71821811-71821830 Msa0469820:three_prime_UTR 30.0%
GCCTTTCATTCAAGCATAAA+GGG - chr3_3:71819299-71819318 None:intergenic 35.0%
AGCCTTTCATTCAAGCATAA+AGG - chr3_3:71819300-71819319 None:intergenic 35.0%
GAATGAAAGGCTTCTTCTAT+TGG + chr3_3:71819308-71819327 Msa0469820:CDS 35.0%
!!! TGAACTAGCAACTTTTTTGG+AGG - chr3_3:71819391-71819410 None:intergenic 35.0%
! ATGATTTTACCTTCTGGACT+AGG - chr3_3:71819555-71819574 None:intergenic 35.0%
GAATCAGGCATGATCATATT+CGG - chr3_3:71819772-71819791 None:intergenic 35.0%
GTAGTAGTAAGACATAAGGA+TGG + chr3_3:71820093-71820112 Msa0469820:intron 35.0%
! TTGGGTCATTGTTTTCTTCT+TGG + chr3_3:71820132-71820151 Msa0469820:intron 35.0%
! TTATGGTATGTGGATTTGGT+AGG + chr3_3:71820231-71820250 Msa0469820:intron 35.0%
! TATGGTATGTGGATTTGGTA+GGG + chr3_3:71820232-71820251 Msa0469820:intron 35.0%
GTGTATTAGATGCTGACATA+AGG - chr3_3:71820557-71820576 None:intergenic 35.0%
TTACTAACAGTGCAACTAGT+TGG + chr3_3:71820730-71820749 Msa0469820:intron 35.0%
TACTAACAGTGCAACTAGTT+GGG + chr3_3:71820731-71820750 Msa0469820:intron 35.0%
CATTCTCATTTCTCATTCCA+AGG - chr3_3:71820877-71820896 None:intergenic 35.0%
GTTCTTCTTGCAATTCTCTA+GGG - chr3_3:71821002-71821021 None:intergenic 35.0%
AAAGAGAAAGAAGAAGACCA+TGG + chr3_3:71821213-71821232 Msa0469820:CDS 35.0%
!! TGCATGCTTTGAGTATTTTG+GGG + chr3_3:71821538-71821557 Msa0469820:three_prime_UTR 35.0%
!! CTTTGAGTATTTTGGGGAAT+GGG + chr3_3:71821544-71821563 Msa0469820:three_prime_UTR 35.0%
!! TTTGAGTATTTTGGGGAATG+GGG + chr3_3:71821545-71821564 Msa0469820:three_prime_UTR 35.0%
TGGGAAACAATGAAGCAAAA+GGG + chr3_3:71821763-71821782 Msa0469820:three_prime_UTR 35.0%
TCCCTATTGTATAATGCTAC+AGG + chr3_3:71821786-71821805 Msa0469820:three_prime_UTR 35.0%
GTCCTGTAGCATTATACAAT+AGG - chr3_3:71821791-71821810 None:intergenic 35.0%
TTAGGGTGGAAATTGCAACA+TGG - chr3_3:71819255-71819274 None:intergenic 40.0%
CAACAACTACTTGTTTAGGG+TGG - chr3_3:71819269-71819288 None:intergenic 40.0%
CACCCTAAACAAGTAGTTGT+TGG + chr3_3:71819267-71819286 Msa0469820:CDS 40.0%
CGCCAACAACTACTTGTTTA+GGG - chr3_3:71819272-71819291 None:intergenic 40.0%
ACGCCAACAACTACTTGTTT+AGG - chr3_3:71819273-71819292 None:intergenic 40.0%
GCCCTTTATGCTTGAATGAA+AGG + chr3_3:71819295-71819314 Msa0469820:CDS 40.0%
! CTTCTATTGGCTGCAAAACA+AGG + chr3_3:71819321-71819340 Msa0469820:CDS 40.0%
! TATTGGCTGCAAAACAAGGT+CGG + chr3_3:71819325-71819344 Msa0469820:CDS 40.0%
AAGAAGAACGGTGGTTATGA+TGG - chr3_3:71819356-71819375 None:intergenic 40.0%
AAGATGAAGAAGAAGAACGG+TGG - chr3_3:71819365-71819384 None:intergenic 40.0%
!! ATTTGGTGGCTTTGTGTGTA+CGG - chr3_3:71819430-71819449 None:intergenic 40.0%
TTAATCTTTGCGGTCTCATG+TGG + chr3_3:71819798-71819817 Msa0469820:intron 40.0%
CATGTTTGAATGTCTTCACG+TGG + chr3_3:71820020-71820039 Msa0469820:intron 40.0%
ATGTCTTCACGTGGAAGATT+AGG + chr3_3:71820029-71820048 Msa0469820:intron 40.0%
AGGATGGGAAAGAAAGCTTT+CGG + chr3_3:71820109-71820128 Msa0469820:intron 40.0%
! TCATTGTTTTCTTCTTGGCG+TGG + chr3_3:71820137-71820156 Msa0469820:intron 40.0%
! TGTGGATTTGGTAGGGAATT+AGG + chr3_3:71820239-71820258 Msa0469820:intron 40.0%
! GTGGATTTGGTAGGGAATTA+GGG + chr3_3:71820240-71820259 Msa0469820:intron 40.0%
ACTAACAGTGCAACTAGTTG+GGG + chr3_3:71820732-71820751 Msa0469820:intron 40.0%
TCATTTCTCATTCCAAGGGT+AGG - chr3_3:71820872-71820891 None:intergenic 40.0%
GGTTCTTCTTGCAATTCTCT+AGG - chr3_3:71821003-71821022 None:intergenic 40.0%
GAATTGCAAGAAGAACCATG+AGG + chr3_3:71821006-71821025 Msa0469820:CDS 40.0%
! TGGTCTCTAATTTGTCCTCA+TGG - chr3_3:71821024-71821043 None:intergenic 40.0%
! TTTTCTTCCACCTTATGAGC+TGG - chr3_3:71821114-71821133 None:intergenic 40.0%
CTCATAAGGTGGAAGAAAAC+TGG + chr3_3:71821115-71821134 Msa0469820:CDS 40.0%
ATAAGGTGGAAGAAAACTGG+AGG + chr3_3:71821118-71821137 Msa0469820:CDS 40.0%
!! GCTTTGAGTATTTTGGGGAA+TGG + chr3_3:71821543-71821562 Msa0469820:three_prime_UTR 40.0%
AAGACAGTACAGGCTGTAAA+AGG + chr3_3:71821623-71821642 Msa0469820:three_prime_UTR 40.0%
AGACAGTACAGGCTGTAAAA+GGG + chr3_3:71821624-71821643 Msa0469820:three_prime_UTR 40.0%
TCCTTCACACTTCACAGTAT+GGG - chr3_3:71821701-71821720 None:intergenic 40.0%
ATCCTTCACACTTCACAGTA+TGG - chr3_3:71821702-71821721 None:intergenic 40.0%
!!! ACCTAACATGGGGAGTATTT+TGG - chr3_3:71821735-71821754 None:intergenic 40.0%
CTTCATTGTTTCCCACTACA+TGG - chr3_3:71821758-71821777 None:intergenic 40.0%
GTGGGAAACAATGAAGCAAA+AGG + chr3_3:71821762-71821781 Msa0469820:three_prime_UTR 40.0%
AAGAACGGTGGTTATGATGG+TGG - chr3_3:71819353-71819372 None:intergenic 45.0%
TCTCGTCACTGCAAATCTCA+TGG + chr3_3:71819504-71819523 Msa0469820:CDS 45.0%
GATATCTCTCCTAGTCCAGA+AGG + chr3_3:71819543-71819562 Msa0469820:CDS 45.0%
!!! TTTTCATGGTTTGGTTGGGG+AGG + chr3_3:71819939-71819958 Msa0469820:intron 45.0%
TGGGAAAGAAAGCTTTCGGT+TGG + chr3_3:71820113-71820132 Msa0469820:intron 45.0%
GGGAAAGAAAGCTTTCGGTT+GGG + chr3_3:71820114-71820133 Msa0469820:intron 45.0%
TACTAATACCATGCTGCCGA+CGG - chr3_3:71820615-71820634 None:intergenic 45.0%
! CTTCTTGCAATTCTCTAGGG+AGG - chr3_3:71820999-71821018 None:intergenic 45.0%
TGCATGCTCTATCACACTCT+TGG - chr3_3:71821044-71821063 None:intergenic 45.0%
CAAAGTCACGTGACACATTC+AGG + chr3_3:71821062-71821081 Msa0469820:CDS 45.0%
ACACATTCAGGTGGAGAAAG+AGG + chr3_3:71821074-71821093 Msa0469820:CDS 45.0%
TTCAGGTGGAGAAAGAGGAT+TGG + chr3_3:71821079-71821098 Msa0469820:CDS 45.0%
GTCACATGTTCCAGCTCATA+AGG + chr3_3:71821101-71821120 Msa0469820:CDS 45.0%
ACATGTTCCAGCTCATAAGG+TGG + chr3_3:71821104-71821123 Msa0469820:CDS 45.0%
GGAGCAGTGAAAGTCAGAAA+AGG + chr3_3:71821172-71821191 Msa0469820:CDS 45.0%
CAGTGAAAGTCAGAAAAGGC+TGG + chr3_3:71821176-71821195 Msa0469820:CDS 45.0%
AAGTCAGAAAAGGCTGGATG+AGG + chr3_3:71821182-71821201 Msa0469820:CDS 45.0%
TCCCATACTGTGAAGTGTGA+AGG + chr3_3:71821697-71821716 Msa0469820:three_prime_UTR 45.0%
GCCAAAATACTCCCCATGTT+AGG + chr3_3:71821731-71821750 Msa0469820:three_prime_UTR 45.0%
CACTACATGGACCTAACATG+GGG - chr3_3:71821745-71821764 None:intergenic 45.0%
CCACTACATGGACCTAACAT+GGG - chr3_3:71821746-71821765 None:intergenic 45.0%
CCATGTTAGGTCCATGTAGT+GGG + chr3_3:71821744-71821763 Msa0469820:three_prime_UTR 45.0%
!!! TAATTTTAATTTATACTTTT+TGG - chr3_3:71820786-71820805 None:intergenic 5.0%
! GGTTTGGTTGGGGAGGAATT+TGG + chr3_3:71819946-71819965 Msa0469820:intron 50.0%
TATCAAATCCGTCGGCAGCA+TGG + chr3_3:71820604-71820623 Msa0469820:intron 50.0%
TTTGTGTGGGAACCTACCCT+TGG + chr3_3:71820857-71820876 Msa0469820:intron 50.0%
! GGAAAAGAGCACGGTCTCAA+AGG + chr3_3:71820967-71820986 Msa0469820:CDS 50.0%
! CTTGCAATTCTCTAGGGAGG+AGG - chr3_3:71820996-71821015 None:intergenic 50.0%
AGTCACGTGACACATTCAGG+TGG + chr3_3:71821065-71821084 Msa0469820:CDS 50.0%
TGTATGCCACGTAGGCAACA+AGG + chr3_3:71821141-71821160 Msa0469820:CDS 50.0%
CTTCAACCTTGTTGCCTACG+TGG - chr3_3:71821150-71821169 None:intergenic 50.0%
CACGTAGGCAACAAGGTTGA+AGG + chr3_3:71821148-71821167 Msa0469820:CDS 50.0%
GTAGGCAACAAGGTTGAAGG+AGG + chr3_3:71821151-71821170 Msa0469820:CDS 50.0%
CCCATGTTAGGTCCATGTAG+TGG + chr3_3:71821743-71821762 Msa0469820:three_prime_UTR 50.0%
CCCACTACATGGACCTAACA+TGG - chr3_3:71821747-71821766 None:intergenic 50.0%
AAGGAAATGGGGTAGCCTCG+TGG + chr3_3:71820945-71820964 Msa0469820:CDS 55.0%
AGGAAATGGGGTAGCCTCGT+GGG + chr3_3:71820946-71820965 Msa0469820:CDS 55.0%
AGCCTCGTGGGAAAAGAGCA+CGG + chr3_3:71820958-71820977 Msa0469820:CDS 55.0%
! ACTGGAGGTGTATGCCACGT+AGG + chr3_3:71821133-71821152 Msa0469820:CDS 55.0%
! GACCGTGCTCTTTTCCCACG+AGG - chr3_3:71820963-71820982 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 71819024 71821834 71819024 ID=Msa0469820;Name=Msa0469820
chr3_3 mRNA 71819024 71821834 71819024 ID=Msa0469820-mRNA-1;Parent=Msa0469820;Name=Msa0469820-mRNA-1;_AED=0.03;_eAED=0.03;_QI=198|1|1|1|1|1|2|594|221
chr3_3 exon 71819024 71819564 71819024 ID=Msa0469820-mRNA-1:exon:17684;Parent=Msa0469820-mRNA-1
chr3_3 exon 71820918 71821834 71820918 ID=Msa0469820-mRNA-1:exon:17685;Parent=Msa0469820-mRNA-1
chr3_3 five_prime_UTR 71819024 71819221 71819024 ID=Msa0469820-mRNA-1:five_prime_utr;Parent=Msa0469820-mRNA-1
chr3_3 CDS 71819222 71819564 71819222 ID=Msa0469820-mRNA-1:cds;Parent=Msa0469820-mRNA-1
chr3_3 CDS 71820918 71821240 71820918 ID=Msa0469820-mRNA-1:cds;Parent=Msa0469820-mRNA-1
chr3_3 three_prime_UTR 71821241 71821834 71821241 ID=Msa0469820-mRNA-1:three_prime_utr;Parent=Msa0469820-mRNA-1
Gene Sequence

>Msa0469820

ATGAATAGGAATGGATATAGGGATGAAAAACCATGTTGCAATTTCCACCCTAAACAAGTAGTTGTTGGCGTATGCCCTTTATGCTTGAATGAAAGGCTTCTTCTATTGGCTGCAAAACAAGGTCGGCGCCACCATCATAACCACCGTTCTTCTTCTTCATCTTCTACCTCCAAAAAAGTTGCTAGTTCACAAAAGCTTCAAAGTTCCGTACACACAAAGCCACCAAATTCTTCTTCTATTCATAAGATCTTTGCTTTTGGTTCTCTCTTCACTCGTCTTGAATCTCGTCACTGCAAATCTCATGGCAACTTTCACTATGATGATATCTCTCCTAGTCCAGAAGAAGACTCATTCATATCAATCAAGTTTGAAGGAAATGGGGTAGCCTCGTGGGAAAAGAGCACGGTCTCAAAGGTGTCCTCCTCCCTAGAGAATTGCAAGAAGAACCATGAGGACAAATTAGAGACCAAGAGTGTGATAGAGCATGCAAAGTCACGTGACACATTCAGGTGGAGAAAGAGGATTGGTCACATGTTCCAGCTCATAAGGTGGAAGAAAACTGGAGGTGTATGCCACGTAGGCAACAAGGTTGAAGGAGGAGCAGTGAAAGTCAGAAAAGGCTGGATGAGGACTCTGACAAAGAGAAAGAAGAAGACCATGGAATAA
Protein sequence

>Msa0469820

MNRNGYRDEKPCCNFHPKQVVVGVCPLCLNERLLLLAAKQGRRHHHNHRSSSSSSTSKKVASSQKLQSSVHTKPPNSSSIHKIFAFGSLFTRLESRHCKSHGNFHYDDISPSPEEDSFISIKFEGNGVASWEKSTVSKVSSSLENCKKNHEDKLETKSVIEHAKSRDTFRWRKRIGHMFQLIRWKKTGGVCHVGNKVEGGAVKVRKGWMRTLTKRKKKTME*