AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0029540


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0029540 MtrunA17_Chr1g0194141 97.521 121 3 0 1 121 1 121 7.34e-86 245
Msa0029540 MtrunA17_Chr7g0260981 67.797 118 38 0 1 118 1 118 1.73e-59 179
Msa0029540 MtrunA17_Chr4g0069171 68.644 118 37 0 1 118 1 118 2.20e-59 178
Msa0029540 MtrunA17_Chr2g0288531 54.310 116 53 0 5 120 4 119 1.52e-43 138
Msa0029540 MtrunA17_Chr4g0024721 54.310 116 53 0 3 118 2 117 1.59e-43 138
Msa0029540 MtrunA17_Chr2g0322571 55.263 114 51 0 5 118 4 117 2.70e-42 135
Msa0029540 MtrunA17_Chr4g0056301 52.586 116 55 0 5 120 4 119 2.43e-41 133
Msa0029540 MtrunA17_Chr4g0019141 45.536 112 59 2 6 115 5 116 6.19e-27 96.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0029540 AT3G06420.1 69.027 113 35 0 6 118 7 119 8.59e-57 172
Msa0029540 AT3G15580.1 70.536 112 33 0 7 118 4 115 1.15e-56 172
Msa0029540 AT4G21980.1 57.265 117 50 0 5 121 4 120 5.00e-45 142
Msa0029540 AT4G21980.2 57.265 117 50 0 5 121 19 135 1.19e-44 142
Msa0029540 AT1G62040.1 56.897 116 50 0 3 118 2 117 2.21e-44 140
Msa0029540 AT2G45170.2 53.719 121 56 0 1 121 1 121 3.29e-44 140
Msa0029540 AT2G45170.1 53.719 121 56 0 1 121 1 121 3.29e-44 140
Msa0029540 AT4G04620.3 53.448 116 54 0 5 120 4 119 2.73e-42 135
Msa0029540 AT4G04620.2 53.448 116 54 0 5 120 4 119 2.73e-42 135
Msa0029540 AT4G04620.1 53.448 116 54 0 5 120 4 119 2.73e-42 135
Msa0029540 AT2G05630.1 53.509 114 53 0 5 118 4 117 6.18e-42 134
Msa0029540 AT4G16520.2 54.386 114 52 0 5 118 4 117 1.46e-41 134
Msa0029540 AT4G16520.1 54.386 114 52 0 5 118 4 117 1.46e-41 134
Msa0029540 AT3G60640.1 52.174 115 55 0 6 120 5 119 5.32e-41 132
Msa0029540 AT2G05630.2 52.778 108 51 0 11 118 54 161 1.39e-38 127
Msa0029540 AT4G16520.3 55.682 88 39 0 31 118 30 117 1.06e-30 106
Msa0029540 AT1G62040.2 52.688 93 44 0 3 95 2 94 1.61e-29 102

Find 35 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGGATTCGATCGGGGTATT+TGG 0.168599 1_1:+54829913 None:intergenic
ATGAATAAGAATGCAATAAT+TGG 0.305135 1_1:+54829233 None:intergenic
TACCAAACGTAAACTCGTTC+TGG 0.338661 1_1:+54830050 None:intergenic
TCCAGCTGGTAAATTAAGTC+TGG 0.354424 1_1:+54829589 None:intergenic
CAAATGCGATCTGCCTCATT+TGG 0.355422 1_1:-54829782 Msa0029540:CDS
ATGTATAACATTGAACCATT+TGG 0.355581 1_1:-54829198 Msa0029540:three_prime_UTR
TGTATAACATTGAACCATTT+GGG 0.369658 1_1:-54829197 Msa0029540:three_prime_UTR
AAGAATACTTTACCTCAAAC+TGG 0.387404 1_1:-54829545 Msa0029540:intron
GGACTTACGGGGATTCGATC+GGG 0.390336 1_1:+54829905 None:intergenic
ATATTTGTTGTATTGTTGTA+TGG 0.397542 1_1:-54829264 Msa0029540:three_prime_UTR
CAAATTCATAATAGCCCAAA+TGG 0.408070 1_1:+54829183 None:intergenic
TATAGCACTGAGAAAACCTT+TGG 0.412532 1_1:-54829366 Msa0029540:CDS
CATGATCATTGATGATGTAT+AGG 0.421743 1_1:-54829154 Msa0029540:three_prime_UTR
TGCTATTGCAGCTAGTATGA+TGG 0.424492 1_1:-54829439 Msa0029540:intron
GCAAGTTTATTACCAGTTTG+AGG 0.425620 1_1:+54829533 None:intergenic
CAAATGAGGCAGATCGCATT+TGG 0.427187 1_1:+54829783 None:intergenic
CAATACAACAAATATTTACA+AGG 0.439972 1_1:+54829272 None:intergenic
AATAGTTGACATTTATGTAC+AGG 0.446245 1_1:+54829341 None:intergenic
TCCAGACTTAATTTACCAGC+TGG 0.456823 1_1:-54829590 Msa0029540:CDS
TCTTCTTCTTCAGGTGATTG+TGG 0.457646 1_1:-54829815 Msa0029540:intron
CTCTGTTTATAGATCATTCA+AGG 0.464700 1_1:-54829415 Msa0029540:CDS
TTCCAGAACGAGTTTACGTT+TGG 0.466165 1_1:-54830052 Msa0029540:intron
ACAAGGTTTCTGATATAAGA+AGG 0.484325 1_1:+54829289 None:intergenic
TAGCAAGACAAGAGTTGCTC+TGG 0.491262 1_1:+54830109 None:intergenic
GGGACTTACGGGGATTCGAT+CGG 0.493031 1_1:+54829904 None:intergenic
ACATTAATCAACAAGGATGT+AGG 0.509610 1_1:+54829089 None:intergenic
CTTTGTTACATTAATCAACA+AGG 0.538506 1_1:+54829082 None:intergenic
TTATAGATCATTCAAGGATG+AGG 0.555700 1_1:-54829409 Msa0029540:CDS
AGATCATTCAAGGATGAGGA+TGG 0.557576 1_1:-54829405 Msa0029540:CDS
CATTTATGTACAGGATCCAA+AGG 0.566947 1_1:+54829350 None:intergenic
GTTCCACGAGACTTGTCAGT+TGG 0.569472 1_1:-54829635 Msa0029540:CDS
AATAAGAATGCAATAATTGG+TGG 0.588342 1_1:+54829236 None:intergenic
GACTTACGGGGATTCGATCG+GGG 0.607489 1_1:+54829906 None:intergenic
TTTCTGATATAAGAAGGTAG+TGG 0.639447 1_1:+54829295 None:intergenic
TGTCCAACTGACAAGTCTCG+TGG 0.654531 1_1:+54829632 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAATTAGATTTAAGATT+AGG + chr1_1:54829662-54829681 None:intergenic 10.0%
!!! AAAAAATTAGATTTAAGATT+AGG + chr1_1:54829662-54829681 None:intergenic 10.0%
!! AAGCCAAATTGAAATTAAAT+TGG + chr1_1:54829311-54829330 None:intergenic 20.0%
!!! AATAATTTTTCTTCTTCTTC+AGG - chr1_1:54829340-54829359 Msa0029540:exon 20.0%
!! CAATACAACAAATATTTACA+AGG + chr1_1:54829895-54829914 None:intergenic 20.0%
!!! ATATTTGTTGTATTGTTGTA+TGG - chr1_1:54829900-54829919 Msa0029540:intron 20.0%
!! ATGAATAAGAATGCAATAAT+TGG + chr1_1:54829934-54829953 None:intergenic 20.0%
!! AAGCCAAATTGAAATTAAAT+TGG + chr1_1:54829311-54829330 None:intergenic 20.0%
!!! AATAATTTTTCTTCTTCTTC+AGG - chr1_1:54829340-54829359 Msa0029540:exon 20.0%
!! CAATACAACAAATATTTACA+AGG + chr1_1:54829895-54829914 None:intergenic 20.0%
!!! ATATTTGTTGTATTGTTGTA+TGG - chr1_1:54829900-54829919 Msa0029540:intron 20.0%
!! ATGAATAAGAATGCAATAAT+TGG + chr1_1:54829934-54829953 None:intergenic 20.0%
! TACGAAAATCAATGATTGAT+GGG + chr1_1:54829283-54829302 None:intergenic 25.0%
! ATACGAAAATCAATGATTGA+TGG + chr1_1:54829284-54829303 None:intergenic 25.0%
! CCAAATTGAAATTAAATTGG+AGG + chr1_1:54829308-54829327 None:intergenic 25.0%
!! CCTCCAATTTAATTTCAATT+TGG - chr1_1:54829305-54829324 Msa0029540:three_prime_UTR 25.0%
! AATAGTTGACATTTATGTAC+AGG + chr1_1:54829826-54829845 None:intergenic 25.0%
! AATAAGAATGCAATAATTGG+TGG + chr1_1:54829931-54829950 None:intergenic 25.0%
! ATGTATAACATTGAACCATT+TGG - chr1_1:54829966-54829985 Msa0029540:intron 25.0%
! TGTATAACATTGAACCATTT+GGG - chr1_1:54829967-54829986 Msa0029540:intron 25.0%
! CTTTGTTACATTAATCAACA+AGG + chr1_1:54830085-54830104 None:intergenic 25.0%
! TACGAAAATCAATGATTGAT+GGG + chr1_1:54829283-54829302 None:intergenic 25.0%
! ATACGAAAATCAATGATTGA+TGG + chr1_1:54829284-54829303 None:intergenic 25.0%
! CCAAATTGAAATTAAATTGG+AGG + chr1_1:54829308-54829327 None:intergenic 25.0%
!! CCTCCAATTTAATTTCAATT+TGG - chr1_1:54829305-54829324 Msa0029540:three_prime_UTR 25.0%
! AATAGTTGACATTTATGTAC+AGG + chr1_1:54829826-54829845 None:intergenic 25.0%
! AATAAGAATGCAATAATTGG+TGG + chr1_1:54829931-54829950 None:intergenic 25.0%
! ATGTATAACATTGAACCATT+TGG - chr1_1:54829966-54829985 Msa0029540:intron 25.0%
! TGTATAACATTGAACCATTT+GGG - chr1_1:54829967-54829986 Msa0029540:intron 25.0%
! CTTTGTTACATTAATCAACA+AGG + chr1_1:54830085-54830104 None:intergenic 25.0%
TAGATCTTCGACACAAAAAT+GGG - chr1_1:54829076-54829095 Msa0029540:three_prime_UTR 30.0%
!!! TACTTTTTCTTCTCCAAATG+AGG + chr1_1:54829398-54829417 None:intergenic 30.0%
AAGAATACTTTACCTCAAAC+TGG - chr1_1:54829619-54829638 Msa0029540:CDS 30.0%
CTCTGTTTATAGATCATTCA+AGG - chr1_1:54829749-54829768 Msa0029540:intron 30.0%
TTATAGATCATTCAAGGATG+AGG - chr1_1:54829755-54829774 Msa0029540:intron 30.0%
! TTTTCTCAGTGCTATAATAC+AGG + chr1_1:54829794-54829813 None:intergenic 30.0%
TTTCTGATATAAGAAGGTAG+TGG + chr1_1:54829872-54829891 None:intergenic 30.0%
ACAAGGTTTCTGATATAAGA+AGG + chr1_1:54829878-54829897 None:intergenic 30.0%
CAAATTCATAATAGCCCAAA+TGG + chr1_1:54829984-54830003 None:intergenic 30.0%
!! CATGATCATTGATGATGTAT+AGG - chr1_1:54830010-54830029 Msa0029540:intron 30.0%
ACATTAATCAACAAGGATGT+AGG + chr1_1:54830078-54830097 None:intergenic 30.0%
TAGATCTTCGACACAAAAAT+GGG - chr1_1:54829076-54829095 Msa0029540:three_prime_UTR 30.0%
!!! TACTTTTTCTTCTCCAAATG+AGG + chr1_1:54829398-54829417 None:intergenic 30.0%
AAGAATACTTTACCTCAAAC+TGG - chr1_1:54829619-54829638 Msa0029540:CDS 30.0%
CTCTGTTTATAGATCATTCA+AGG - chr1_1:54829749-54829768 Msa0029540:intron 30.0%
TTATAGATCATTCAAGGATG+AGG - chr1_1:54829755-54829774 Msa0029540:intron 30.0%
! TTTTCTCAGTGCTATAATAC+AGG + chr1_1:54829794-54829813 None:intergenic 30.0%
TTTCTGATATAAGAAGGTAG+TGG + chr1_1:54829872-54829891 None:intergenic 30.0%
ACAAGGTTTCTGATATAAGA+AGG + chr1_1:54829878-54829897 None:intergenic 30.0%
CAAATTCATAATAGCCCAAA+TGG + chr1_1:54829984-54830003 None:intergenic 30.0%
!! CATGATCATTGATGATGTAT+AGG - chr1_1:54830010-54830029 Msa0029540:intron 30.0%
ACATTAATCAACAAGGATGT+AGG + chr1_1:54830078-54830097 None:intergenic 30.0%
CTAGATCTTCGACACAAAAA+TGG - chr1_1:54829075-54829094 Msa0029540:three_prime_UTR 35.0%
TCAATGATTGATGGGACTTA+CGG + chr1_1:54829275-54829294 None:intergenic 35.0%
GCAAGTTTATTACCAGTTTG+AGG + chr1_1:54829634-54829653 None:intergenic 35.0%
CAACATGAAAAAGCAACAAG+AGG + chr1_1:54829692-54829711 None:intergenic 35.0%
! TATAGCACTGAGAAAACCTT+TGG - chr1_1:54829798-54829817 Msa0029540:CDS 35.0%
CATTTATGTACAGGATCCAA+AGG + chr1_1:54829817-54829836 None:intergenic 35.0%
CTAGATCTTCGACACAAAAA+TGG - chr1_1:54829075-54829094 Msa0029540:three_prime_UTR 35.0%
TCAATGATTGATGGGACTTA+CGG + chr1_1:54829275-54829294 None:intergenic 35.0%
GCAAGTTTATTACCAGTTTG+AGG + chr1_1:54829634-54829653 None:intergenic 35.0%
CAACATGAAAAAGCAACAAG+AGG + chr1_1:54829692-54829711 None:intergenic 35.0%
! TATAGCACTGAGAAAACCTT+TGG - chr1_1:54829798-54829817 Msa0029540:CDS 35.0%
CATTTATGTACAGGATCCAA+AGG + chr1_1:54829817-54829836 None:intergenic 35.0%
ATCTTCGACACAAAAATGGG+AGG - chr1_1:54829079-54829098 Msa0029540:three_prime_UTR 40.0%
TTCCAGAACGAGTTTACGTT+TGG - chr1_1:54829112-54829131 Msa0029540:three_prime_UTR 40.0%
TACCAAACGTAAACTCGTTC+TGG + chr1_1:54829117-54829136 None:intergenic 40.0%
! TTTTCGCGTGTGTAGAACAA+AGG - chr1_1:54829203-54829222 Msa0029540:three_prime_UTR 40.0%
AATGATTGATGGGACTTACG+GGG + chr1_1:54829273-54829292 None:intergenic 40.0%
CAATGATTGATGGGACTTAC+GGG + chr1_1:54829274-54829293 None:intergenic 40.0%
TCTTCTTCTTCAGGTGATTG+TGG - chr1_1:54829349-54829368 Msa0029540:exon 40.0%
TCCAGACTTAATTTACCAGC+TGG - chr1_1:54829574-54829593 Msa0029540:CDS 40.0%
TCCAGCTGGTAAATTAAGTC+TGG + chr1_1:54829578-54829597 None:intergenic 40.0%
TGCTATTGCAGCTAGTATGA+TGG - chr1_1:54829725-54829744 Msa0029540:intron 40.0%
AGATCATTCAAGGATGAGGA+TGG - chr1_1:54829759-54829778 Msa0029540:intron 40.0%
ATCTTCGACACAAAAATGGG+AGG - chr1_1:54829079-54829098 Msa0029540:three_prime_UTR 40.0%
TTCCAGAACGAGTTTACGTT+TGG - chr1_1:54829112-54829131 Msa0029540:three_prime_UTR 40.0%
TACCAAACGTAAACTCGTTC+TGG + chr1_1:54829117-54829136 None:intergenic 40.0%
! TTTTCGCGTGTGTAGAACAA+AGG - chr1_1:54829203-54829222 Msa0029540:three_prime_UTR 40.0%
AATGATTGATGGGACTTACG+GGG + chr1_1:54829273-54829292 None:intergenic 40.0%
CAATGATTGATGGGACTTAC+GGG + chr1_1:54829274-54829293 None:intergenic 40.0%
TCTTCTTCTTCAGGTGATTG+TGG - chr1_1:54829349-54829368 Msa0029540:exon 40.0%
TCCAGACTTAATTTACCAGC+TGG - chr1_1:54829574-54829593 Msa0029540:CDS 40.0%
TCCAGCTGGTAAATTAAGTC+TGG + chr1_1:54829578-54829597 None:intergenic 40.0%
TGCTATTGCAGCTAGTATGA+TGG - chr1_1:54829725-54829744 Msa0029540:intron 40.0%
AGATCATTCAAGGATGAGGA+TGG - chr1_1:54829759-54829778 Msa0029540:intron 40.0%
TAGCAAGACAAGAGTTGCTC+TGG + chr1_1:54829058-54829077 None:intergenic 45.0%
TTCGACACAAAAATGGGAGG+AGG - chr1_1:54829082-54829101 Msa0029540:three_prime_UTR 45.0%
CAAATGAGGCAGATCGCATT+TGG + chr1_1:54829384-54829403 None:intergenic 45.0%
CAAATGCGATCTGCCTCATT+TGG - chr1_1:54829382-54829401 Msa0029540:CDS 45.0%
! ACAAAGAGAGCTTTTCCAGC+TGG + chr1_1:54829592-54829611 None:intergenic 45.0%
TAGCAAGACAAGAGTTGCTC+TGG + chr1_1:54829058-54829077 None:intergenic 45.0%
TTCGACACAAAAATGGGAGG+AGG - chr1_1:54829082-54829101 Msa0029540:three_prime_UTR 45.0%
CAAATGAGGCAGATCGCATT+TGG + chr1_1:54829384-54829403 None:intergenic 45.0%
CAAATGCGATCTGCCTCATT+TGG - chr1_1:54829382-54829401 Msa0029540:CDS 45.0%
! ACAAAGAGAGCTTTTCCAGC+TGG + chr1_1:54829592-54829611 None:intergenic 45.0%
!! ATAAGATAAAATTATTAAAA+AGG + chr1_1:54829155-54829174 None:intergenic 5.0%
!! ATAAGATAAAATTATTAAAA+AGG + chr1_1:54829155-54829174 None:intergenic 5.0%
GTTCCACGAGACTTGTCAGT+TGG - chr1_1:54829529-54829548 Msa0029540:intron 50.0%
TGTCCAACTGACAAGTCTCG+TGG + chr1_1:54829535-54829554 None:intergenic 50.0%
GTTCCACGAGACTTGTCAGT+TGG - chr1_1:54829529-54829548 Msa0029540:intron 50.0%
TGTCCAACTGACAAGTCTCG+TGG + chr1_1:54829535-54829554 None:intergenic 50.0%
! GGGGATTCGATCGGGGTATT+TGG + chr1_1:54829254-54829273 None:intergenic 55.0%
GACTTACGGGGATTCGATCG+GGG + chr1_1:54829261-54829280 None:intergenic 55.0%
GGACTTACGGGGATTCGATC+GGG + chr1_1:54829262-54829281 None:intergenic 55.0%
GGGACTTACGGGGATTCGAT+CGG + chr1_1:54829263-54829282 None:intergenic 55.0%
! GGGGATTCGATCGGGGTATT+TGG + chr1_1:54829254-54829273 None:intergenic 55.0%
GACTTACGGGGATTCGATCG+GGG + chr1_1:54829261-54829280 None:intergenic 55.0%
GGACTTACGGGGATTCGATC+GGG + chr1_1:54829262-54829281 None:intergenic 55.0%
GGGACTTACGGGGATTCGAT+CGG + chr1_1:54829263-54829282 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 54829017 54830169 54829017 ID=Msa0029540;Name=Msa0029540
chr1_1 mRNA 54829017 54830169 54829017 ID=Msa0029540-mRNA-1;Parent=Msa0029540;Name=Msa0029540-mRNA-1;_AED=0.13;_eAED=0.13;_QI=77|1|1|1|1|1|5|336|121
chr1_1 exon 54829017 54829450 54829017 ID=Msa0029540-mRNA-1:exon:16394;Parent=Msa0029540-mRNA-1
chr1_1 exon 54829546 54829664 54829546 ID=Msa0029540-mRNA-1:exon:16393;Parent=Msa0029540-mRNA-1
chr1_1 exon 54829772 54829824 54829772 ID=Msa0029540-mRNA-1:exon:16392;Parent=Msa0029540-mRNA-1
chr1_1 exon 54829913 54829968 54829913 ID=Msa0029540-mRNA-1:exon:16391;Parent=Msa0029540-mRNA-1
chr1_1 exon 54830053 54830169 54830053 ID=Msa0029540-mRNA-1:exon:16390;Parent=Msa0029540-mRNA-1
chr1_1 five_prime_UTR 54830093 54830169 54830093 ID=Msa0029540-mRNA-1:five_prime_utr;Parent=Msa0029540-mRNA-1
chr1_1 CDS 54830053 54830092 54830053 ID=Msa0029540-mRNA-1:cds;Parent=Msa0029540-mRNA-1
chr1_1 CDS 54829913 54829968 54829913 ID=Msa0029540-mRNA-1:cds;Parent=Msa0029540-mRNA-1
chr1_1 CDS 54829772 54829824 54829772 ID=Msa0029540-mRNA-1:cds;Parent=Msa0029540-mRNA-1
chr1_1 CDS 54829546 54829664 54829546 ID=Msa0029540-mRNA-1:cds;Parent=Msa0029540-mRNA-1
chr1_1 CDS 54829353 54829450 54829353 ID=Msa0029540-mRNA-1:cds;Parent=Msa0029540-mRNA-1
chr1_1 three_prime_UTR 54829017 54829352 54829017 ID=Msa0029540-mRNA-1:three_prime_utr;Parent=Msa0029540-mRNA-1
Gene Sequence

>Msa0029540

ATGGGAGGAGGTTCTTGTTTCCAGAACGAGTTTACGTTTGAACAAAGGCTTCAAGAATCACGTGATATTATTGCCAAATACCCCGATCGAATCCCCGTGATTGTGGAAAAGTATACCAAATGCGATCTGCCTCATTTGGAGAAGAAAAAATATCTTGTTCCACGAGACTTGTCAGTTGGACATTTCATTCATATTTTGAGTTCCAGACTTAATTTACCAGCTGGAAAAGCTCTCTTTGTATTTGTTAAGAATACTTTACCTCAAACTGCTAGTATGATGGACTCTGTTTATAGATCATTCAAGGATGAGGATGGATTTCTTTACCTGTATTATAGCACTGAGAAAACCTTTGGATCCTGTACATAA

Protein sequence

>Msa0029540

MGGGSCFQNEFTFEQRLQESRDIIAKYPDRIPVIVEKYTKCDLPHLEKKKYLVPRDLSVGHFIHILSSRLNLPAGKALFVFVKNTLPQTASMMDSVYRSFKDEDGFLYLYYSTEKTFGSCT*