AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0138970


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0138970 MtrunA17_Chr1g0194141 97.521 121 3 0 1 121 1 121 7.34e-86 245
Msa0138970 MtrunA17_Chr7g0260981 67.797 118 38 0 1 118 1 118 1.73e-59 179
Msa0138970 MtrunA17_Chr4g0069171 68.644 118 37 0 1 118 1 118 2.20e-59 178
Msa0138970 MtrunA17_Chr2g0288531 54.310 116 53 0 5 120 4 119 1.52e-43 138
Msa0138970 MtrunA17_Chr4g0024721 54.310 116 53 0 3 118 2 117 1.59e-43 138
Msa0138970 MtrunA17_Chr2g0322571 55.263 114 51 0 5 118 4 117 2.70e-42 135
Msa0138970 MtrunA17_Chr4g0056301 52.586 116 55 0 5 120 4 119 2.43e-41 133
Msa0138970 MtrunA17_Chr4g0019141 45.536 112 59 2 6 115 5 116 6.19e-27 96.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0138970 AT3G06420.1 69.027 113 35 0 6 118 7 119 8.59e-57 172
Msa0138970 AT3G15580.1 70.536 112 33 0 7 118 4 115 1.15e-56 172
Msa0138970 AT4G21980.1 57.265 117 50 0 5 121 4 120 5.00e-45 142
Msa0138970 AT4G21980.2 57.265 117 50 0 5 121 19 135 1.19e-44 142
Msa0138970 AT1G62040.1 56.897 116 50 0 3 118 2 117 2.21e-44 140
Msa0138970 AT2G45170.2 53.719 121 56 0 1 121 1 121 3.29e-44 140
Msa0138970 AT2G45170.1 53.719 121 56 0 1 121 1 121 3.29e-44 140
Msa0138970 AT4G04620.3 53.448 116 54 0 5 120 4 119 2.73e-42 135
Msa0138970 AT4G04620.2 53.448 116 54 0 5 120 4 119 2.73e-42 135
Msa0138970 AT4G04620.1 53.448 116 54 0 5 120 4 119 2.73e-42 135
Msa0138970 AT2G05630.1 53.509 114 53 0 5 118 4 117 6.18e-42 134
Msa0138970 AT4G16520.2 54.386 114 52 0 5 118 4 117 1.46e-41 134
Msa0138970 AT4G16520.1 54.386 114 52 0 5 118 4 117 1.46e-41 134
Msa0138970 AT3G60640.1 52.174 115 55 0 6 120 5 119 5.32e-41 132
Msa0138970 AT2G05630.2 52.778 108 51 0 11 118 54 161 1.39e-38 127
Msa0138970 AT4G16520.3 55.682 88 39 0 31 118 30 117 1.06e-30 106
Msa0138970 AT1G62040.2 52.688 93 44 0 3 95 2 94 1.61e-29 102

Find 35 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGGATTCGATCGGGGTATT+TGG 0.168599 1_3:-72198999 None:intergenic
ATGAATAAGAATGCAATAAT+TGG 0.305135 1_3:-72199679 None:intergenic
TACCAAACGTAAACTCGTTC+TGG 0.338661 1_3:-72198862 None:intergenic
TCCAGCTGGTAAATTAAGTC+TGG 0.354424 1_3:-72199323 None:intergenic
CAAATGCGATCTGCCTCATT+TGG 0.355422 1_3:+72199130 Msa0138970:CDS
ATGTATAACATTGAACCATT+TGG 0.355581 1_3:+72199714 Msa0138970:three_prime_UTR
TGTATAACATTGAACCATTT+GGG 0.369658 1_3:+72199715 Msa0138970:three_prime_UTR
AAGAATACTTTACCTCAAAC+TGG 0.387404 1_3:+72199367 Msa0138970:CDS
GGACTTACGGGGATTCGATC+GGG 0.390336 1_3:-72199007 None:intergenic
ATATTTGTTGTATTGTTGTA+TGG 0.397542 1_3:+72199648 Msa0138970:three_prime_UTR
CAAATTCATAATAGCCCAAA+TGG 0.408070 1_3:-72199729 None:intergenic
TATAGCACTGAGAAAACCTT+TGG 0.412532 1_3:+72199546 Msa0138970:CDS
CATGATCATTGATGATGTAT+AGG 0.421743 1_3:+72199758 Msa0138970:three_prime_UTR
TGCTATTGCAGCTAGTATGA+TGG 0.424492 1_3:+72199473 Msa0138970:intron
GCAAGTTTATTACCAGTTTG+AGG 0.425620 1_3:-72199379 None:intergenic
CAAATGAGGCAGATCGCATT+TGG 0.427187 1_3:-72199129 None:intergenic
CAATACAACAAATATTTACA+AGG 0.439972 1_3:-72199640 None:intergenic
AATAGTTGACATTTATGTAC+AGG 0.446245 1_3:-72199571 None:intergenic
TCCAGACTTAATTTACCAGC+TGG 0.456823 1_3:+72199322 Msa0138970:CDS
TCTTCTTCTTCAGGTGATTG+TGG 0.457646 1_3:+72199097 Msa0138970:intron
CTCTGTTTATAGATCATTCA+AGG 0.464700 1_3:+72199497 Msa0138970:CDS
TTCCAGAACGAGTTTACGTT+TGG 0.466165 1_3:+72198860 Msa0138970:CDS
ACAAGGTTTCTGATATAAGA+AGG 0.484325 1_3:-72199623 None:intergenic
TAGCAAGACAAGAGTTGCTC+TGG 0.491262 1_3:-72198803 None:intergenic
GGGACTTACGGGGATTCGAT+CGG 0.493031 1_3:-72199008 None:intergenic
ACATTAATCAACAAGGATGT+AGG 0.509610 1_3:-72199823 None:intergenic
CTTTGTTACATTAATCAACA+AGG 0.538506 1_3:-72199830 None:intergenic
TTATAGATCATTCAAGGATG+AGG 0.555700 1_3:+72199503 Msa0138970:CDS
AGATCATTCAAGGATGAGGA+TGG 0.557576 1_3:+72199507 Msa0138970:CDS
CATTTATGTACAGGATCCAA+AGG 0.566947 1_3:-72199562 None:intergenic
GTTCCACGAGACTTGTCAGT+TGG 0.569472 1_3:+72199277 Msa0138970:CDS
AATAAGAATGCAATAATTGG+TGG 0.588342 1_3:-72199676 None:intergenic
GACTTACGGGGATTCGATCG+GGG 0.607489 1_3:-72199006 None:intergenic
TTTCTGATATAAGAAGGTAG+TGG 0.639447 1_3:-72199617 None:intergenic
TGTCCAACTGACAAGTCTCG+TGG 0.654531 1_3:-72199280 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAATTAGATTTAAGATT+AGG - chr1_3:72199410-72199429 None:intergenic 10.0%
!!! AAAAAATTAGATTTAAGATT+AGG - chr1_3:72199410-72199429 None:intergenic 10.0%
!! AAGCCAAATTGAAATTAAAT+TGG - chr1_3:72199059-72199078 None:intergenic 20.0%
!!! AATAATTTTTCTTCTTCTTC+AGG + chr1_3:72199088-72199107 Msa0138970:intron 20.0%
!! CAATACAACAAATATTTACA+AGG - chr1_3:72199643-72199662 None:intergenic 20.0%
!!! ATATTTGTTGTATTGTTGTA+TGG + chr1_3:72199648-72199667 Msa0138970:three_prime_UTR 20.0%
!! ATGAATAAGAATGCAATAAT+TGG - chr1_3:72199682-72199701 None:intergenic 20.0%
!! AAGCCAAATTGAAATTAAAT+TGG - chr1_3:72199059-72199078 None:intergenic 20.0%
!!! AATAATTTTTCTTCTTCTTC+AGG + chr1_3:72199088-72199107 Msa0138970:intron 20.0%
!! CAATACAACAAATATTTACA+AGG - chr1_3:72199643-72199662 None:intergenic 20.0%
!!! ATATTTGTTGTATTGTTGTA+TGG + chr1_3:72199648-72199667 Msa0138970:three_prime_UTR 20.0%
!! ATGAATAAGAATGCAATAAT+TGG - chr1_3:72199682-72199701 None:intergenic 20.0%
! TACGAAAATCAATGATTGAT+GGG - chr1_3:72199031-72199050 None:intergenic 25.0%
! ATACGAAAATCAATGATTGA+TGG - chr1_3:72199032-72199051 None:intergenic 25.0%
! CCAAATTGAAATTAAATTGG+AGG - chr1_3:72199056-72199075 None:intergenic 25.0%
!! CCTCCAATTTAATTTCAATT+TGG + chr1_3:72199053-72199072 Msa0138970:intron 25.0%
! AATAGTTGACATTTATGTAC+AGG - chr1_3:72199574-72199593 None:intergenic 25.0%
! AATAAGAATGCAATAATTGG+TGG - chr1_3:72199679-72199698 None:intergenic 25.0%
! ATGTATAACATTGAACCATT+TGG + chr1_3:72199714-72199733 Msa0138970:three_prime_UTR 25.0%
! TGTATAACATTGAACCATTT+GGG + chr1_3:72199715-72199734 Msa0138970:three_prime_UTR 25.0%
! CTTTGTTACATTAATCAACA+AGG - chr1_3:72199833-72199852 None:intergenic 25.0%
! TACGAAAATCAATGATTGAT+GGG - chr1_3:72199031-72199050 None:intergenic 25.0%
! ATACGAAAATCAATGATTGA+TGG - chr1_3:72199032-72199051 None:intergenic 25.0%
! CCAAATTGAAATTAAATTGG+AGG - chr1_3:72199056-72199075 None:intergenic 25.0%
!! CCTCCAATTTAATTTCAATT+TGG + chr1_3:72199053-72199072 Msa0138970:intron 25.0%
! AATAGTTGACATTTATGTAC+AGG - chr1_3:72199574-72199593 None:intergenic 25.0%
! AATAAGAATGCAATAATTGG+TGG - chr1_3:72199679-72199698 None:intergenic 25.0%
! ATGTATAACATTGAACCATT+TGG + chr1_3:72199714-72199733 Msa0138970:three_prime_UTR 25.0%
! TGTATAACATTGAACCATTT+GGG + chr1_3:72199715-72199734 Msa0138970:three_prime_UTR 25.0%
! CTTTGTTACATTAATCAACA+AGG - chr1_3:72199833-72199852 None:intergenic 25.0%
TAGATCTTCGACACAAAAAT+GGG + chr1_3:72198824-72198843 Msa0138970:exon 30.0%
!!! TACTTTTTCTTCTCCAAATG+AGG - chr1_3:72199146-72199165 None:intergenic 30.0%
AAGAATACTTTACCTCAAAC+TGG + chr1_3:72199367-72199386 Msa0138970:CDS 30.0%
CTCTGTTTATAGATCATTCA+AGG + chr1_3:72199497-72199516 Msa0138970:CDS 30.0%
TTATAGATCATTCAAGGATG+AGG + chr1_3:72199503-72199522 Msa0138970:CDS 30.0%
! TTTTCTCAGTGCTATAATAC+AGG - chr1_3:72199542-72199561 None:intergenic 30.0%
TTTCTGATATAAGAAGGTAG+TGG - chr1_3:72199620-72199639 None:intergenic 30.0%
ACAAGGTTTCTGATATAAGA+AGG - chr1_3:72199626-72199645 None:intergenic 30.0%
CAAATTCATAATAGCCCAAA+TGG - chr1_3:72199732-72199751 None:intergenic 30.0%
!! CATGATCATTGATGATGTAT+AGG + chr1_3:72199758-72199777 Msa0138970:three_prime_UTR 30.0%
ACATTAATCAACAAGGATGT+AGG - chr1_3:72199826-72199845 None:intergenic 30.0%
TAGATCTTCGACACAAAAAT+GGG + chr1_3:72198824-72198843 Msa0138970:exon 30.0%
!!! TACTTTTTCTTCTCCAAATG+AGG - chr1_3:72199146-72199165 None:intergenic 30.0%
AAGAATACTTTACCTCAAAC+TGG + chr1_3:72199367-72199386 Msa0138970:CDS 30.0%
CTCTGTTTATAGATCATTCA+AGG + chr1_3:72199497-72199516 Msa0138970:CDS 30.0%
TTATAGATCATTCAAGGATG+AGG + chr1_3:72199503-72199522 Msa0138970:CDS 30.0%
! TTTTCTCAGTGCTATAATAC+AGG - chr1_3:72199542-72199561 None:intergenic 30.0%
TTTCTGATATAAGAAGGTAG+TGG - chr1_3:72199620-72199639 None:intergenic 30.0%
ACAAGGTTTCTGATATAAGA+AGG - chr1_3:72199626-72199645 None:intergenic 30.0%
CAAATTCATAATAGCCCAAA+TGG - chr1_3:72199732-72199751 None:intergenic 30.0%
!! CATGATCATTGATGATGTAT+AGG + chr1_3:72199758-72199777 Msa0138970:three_prime_UTR 30.0%
ACATTAATCAACAAGGATGT+AGG - chr1_3:72199826-72199845 None:intergenic 30.0%
CTAGATCTTCGACACAAAAA+TGG + chr1_3:72198823-72198842 Msa0138970:exon 35.0%
TCAATGATTGATGGGACTTA+CGG - chr1_3:72199023-72199042 None:intergenic 35.0%
GCAAGTTTATTACCAGTTTG+AGG - chr1_3:72199382-72199401 None:intergenic 35.0%
CAACATGAAAAAGCAACAAG+AGG - chr1_3:72199440-72199459 None:intergenic 35.0%
! TATAGCACTGAGAAAACCTT+TGG + chr1_3:72199546-72199565 Msa0138970:CDS 35.0%
CATTTATGTACAGGATCCAA+AGG - chr1_3:72199565-72199584 None:intergenic 35.0%
CTAGATCTTCGACACAAAAA+TGG + chr1_3:72198823-72198842 Msa0138970:exon 35.0%
TCAATGATTGATGGGACTTA+CGG - chr1_3:72199023-72199042 None:intergenic 35.0%
GCAAGTTTATTACCAGTTTG+AGG - chr1_3:72199382-72199401 None:intergenic 35.0%
CAACATGAAAAAGCAACAAG+AGG - chr1_3:72199440-72199459 None:intergenic 35.0%
! TATAGCACTGAGAAAACCTT+TGG + chr1_3:72199546-72199565 Msa0138970:CDS 35.0%
CATTTATGTACAGGATCCAA+AGG - chr1_3:72199565-72199584 None:intergenic 35.0%
ATCTTCGACACAAAAATGGG+AGG + chr1_3:72198827-72198846 Msa0138970:exon 40.0%
TTCCAGAACGAGTTTACGTT+TGG + chr1_3:72198860-72198879 Msa0138970:CDS 40.0%
TACCAAACGTAAACTCGTTC+TGG - chr1_3:72198865-72198884 None:intergenic 40.0%
! TTTTCGCGTGTGTAGAACAA+AGG + chr1_3:72198951-72198970 Msa0138970:intron 40.0%
AATGATTGATGGGACTTACG+GGG - chr1_3:72199021-72199040 None:intergenic 40.0%
CAATGATTGATGGGACTTAC+GGG - chr1_3:72199022-72199041 None:intergenic 40.0%
TCTTCTTCTTCAGGTGATTG+TGG + chr1_3:72199097-72199116 Msa0138970:intron 40.0%
TCCAGACTTAATTTACCAGC+TGG + chr1_3:72199322-72199341 Msa0138970:CDS 40.0%
TCCAGCTGGTAAATTAAGTC+TGG - chr1_3:72199326-72199345 None:intergenic 40.0%
TGCTATTGCAGCTAGTATGA+TGG + chr1_3:72199473-72199492 Msa0138970:intron 40.0%
AGATCATTCAAGGATGAGGA+TGG + chr1_3:72199507-72199526 Msa0138970:CDS 40.0%
ATCTTCGACACAAAAATGGG+AGG + chr1_3:72198827-72198846 Msa0138970:exon 40.0%
TTCCAGAACGAGTTTACGTT+TGG + chr1_3:72198860-72198879 Msa0138970:CDS 40.0%
TACCAAACGTAAACTCGTTC+TGG - chr1_3:72198865-72198884 None:intergenic 40.0%
! TTTTCGCGTGTGTAGAACAA+AGG + chr1_3:72198951-72198970 Msa0138970:intron 40.0%
AATGATTGATGGGACTTACG+GGG - chr1_3:72199021-72199040 None:intergenic 40.0%
CAATGATTGATGGGACTTAC+GGG - chr1_3:72199022-72199041 None:intergenic 40.0%
TCTTCTTCTTCAGGTGATTG+TGG + chr1_3:72199097-72199116 Msa0138970:intron 40.0%
TCCAGACTTAATTTACCAGC+TGG + chr1_3:72199322-72199341 Msa0138970:CDS 40.0%
TCCAGCTGGTAAATTAAGTC+TGG - chr1_3:72199326-72199345 None:intergenic 40.0%
TGCTATTGCAGCTAGTATGA+TGG + chr1_3:72199473-72199492 Msa0138970:intron 40.0%
AGATCATTCAAGGATGAGGA+TGG + chr1_3:72199507-72199526 Msa0138970:CDS 40.0%
TAGCAAGACAAGAGTTGCTC+TGG - chr1_3:72198806-72198825 None:intergenic 45.0%
TTCGACACAAAAATGGGAGG+AGG + chr1_3:72198830-72198849 Msa0138970:exon 45.0%
CAAATGAGGCAGATCGCATT+TGG - chr1_3:72199132-72199151 None:intergenic 45.0%
CAAATGCGATCTGCCTCATT+TGG + chr1_3:72199130-72199149 Msa0138970:CDS 45.0%
! ACAAAGAGAGCTTTTCCAGC+TGG - chr1_3:72199340-72199359 None:intergenic 45.0%
TAGCAAGACAAGAGTTGCTC+TGG - chr1_3:72198806-72198825 None:intergenic 45.0%
TTCGACACAAAAATGGGAGG+AGG + chr1_3:72198830-72198849 Msa0138970:exon 45.0%
CAAATGAGGCAGATCGCATT+TGG - chr1_3:72199132-72199151 None:intergenic 45.0%
CAAATGCGATCTGCCTCATT+TGG + chr1_3:72199130-72199149 Msa0138970:CDS 45.0%
! ACAAAGAGAGCTTTTCCAGC+TGG - chr1_3:72199340-72199359 None:intergenic 45.0%
!! ATAAGATAAAATTATTAAAA+AGG - chr1_3:72198903-72198922 None:intergenic 5.0%
!! ATAAGATAAAATTATTAAAA+AGG - chr1_3:72198903-72198922 None:intergenic 5.0%
GTTCCACGAGACTTGTCAGT+TGG + chr1_3:72199277-72199296 Msa0138970:CDS 50.0%
TGTCCAACTGACAAGTCTCG+TGG - chr1_3:72199283-72199302 None:intergenic 50.0%
GTTCCACGAGACTTGTCAGT+TGG + chr1_3:72199277-72199296 Msa0138970:CDS 50.0%
TGTCCAACTGACAAGTCTCG+TGG - chr1_3:72199283-72199302 None:intergenic 50.0%
! GGGGATTCGATCGGGGTATT+TGG - chr1_3:72199002-72199021 None:intergenic 55.0%
GACTTACGGGGATTCGATCG+GGG - chr1_3:72199009-72199028 None:intergenic 55.0%
GGACTTACGGGGATTCGATC+GGG - chr1_3:72199010-72199029 None:intergenic 55.0%
GGGACTTACGGGGATTCGAT+CGG - chr1_3:72199011-72199030 None:intergenic 55.0%
! GGGGATTCGATCGGGGTATT+TGG - chr1_3:72199002-72199021 None:intergenic 55.0%
GACTTACGGGGATTCGATCG+GGG - chr1_3:72199009-72199028 None:intergenic 55.0%
GGACTTACGGGGATTCGATC+GGG - chr1_3:72199010-72199029 None:intergenic 55.0%
GGGACTTACGGGGATTCGAT+CGG - chr1_3:72199011-72199030 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 72198765 72199917 72198765 ID=Msa0138970;Name=Msa0138970
chr1_3 mRNA 72198765 72199917 72198765 ID=Msa0138970-mRNA-1;Parent=Msa0138970;Name=Msa0138970-mRNA-1;_AED=0.13;_eAED=0.13;_QI=77|1|1|1|1|1|5|336|121
chr1_3 exon 72198765 72198881 72198765 ID=Msa0138970-mRNA-1:exon:25228;Parent=Msa0138970-mRNA-1
chr1_3 exon 72198966 72199021 72198966 ID=Msa0138970-mRNA-1:exon:25229;Parent=Msa0138970-mRNA-1
chr1_3 exon 72199110 72199162 72199110 ID=Msa0138970-mRNA-1:exon:25230;Parent=Msa0138970-mRNA-1
chr1_3 exon 72199270 72199388 72199270 ID=Msa0138970-mRNA-1:exon:25231;Parent=Msa0138970-mRNA-1
chr1_3 exon 72199484 72199917 72199484 ID=Msa0138970-mRNA-1:exon:25232;Parent=Msa0138970-mRNA-1
chr1_3 five_prime_UTR 72198765 72198841 72198765 ID=Msa0138970-mRNA-1:five_prime_utr;Parent=Msa0138970-mRNA-1
chr1_3 CDS 72198842 72198881 72198842 ID=Msa0138970-mRNA-1:cds;Parent=Msa0138970-mRNA-1
chr1_3 CDS 72198966 72199021 72198966 ID=Msa0138970-mRNA-1:cds;Parent=Msa0138970-mRNA-1
chr1_3 CDS 72199110 72199162 72199110 ID=Msa0138970-mRNA-1:cds;Parent=Msa0138970-mRNA-1
chr1_3 CDS 72199270 72199388 72199270 ID=Msa0138970-mRNA-1:cds;Parent=Msa0138970-mRNA-1
chr1_3 CDS 72199484 72199581 72199484 ID=Msa0138970-mRNA-1:cds;Parent=Msa0138970-mRNA-1
chr1_3 three_prime_UTR 72199582 72199917 72199582 ID=Msa0138970-mRNA-1:three_prime_utr;Parent=Msa0138970-mRNA-1
Gene Sequence

>Msa0138970

ATGGGAGGAGGTTCTTGTTTCCAGAACGAGTTTACGTTTGAACAAAGGCTTCAAGAATCACGTGATATTATTGCCAAATACCCCGATCGAATCCCCGTGATTGTGGAAAAGTATACCAAATGCGATCTGCCTCATTTGGAGAAGAAAAAATATCTTGTTCCACGAGACTTGTCAGTTGGACATTTCATTCATATTTTGAGTTCCAGACTTAATTTACCAGCTGGAAAAGCTCTCTTTGTATTTGTTAAGAATACTTTACCTCAAACTGCTAGTATGATGGACTCTGTTTATAGATCATTCAAGGATGAGGATGGATTTCTTTACCTGTATTATAGCACTGAGAAAACCTTTGGATCCTGTACATAA

Protein sequence

>Msa0138970

MGGGSCFQNEFTFEQRLQESRDIIAKYPDRIPVIVEKYTKCDLPHLEKKKYLVPRDLSVGHFIHILSSRLNLPAGKALFVFVKNTLPQTASMMDSVYRSFKDEDGFLYLYYSTEKTFGSCT*