AlfalfaGEDB | Msa1310660

M. sativa cultivar zhongmu-4 / Msa1310660

KEGG: -
GO: -
PFAM: PF16884.8

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1310660	sp\|Q39172\|AER_ARATH	71.341	328	90	3	264	588	19	345	5.65e-170	489
Msa1310660	sp\|Q39172\|AER_ARATH	67.742	186	56	3	6	188	5	189	3.40e-79	256
Msa1310660	sp\|Q39172\|AER_ARATH	71.429	77	22	0	189	265	268	344	1.83e-28	119

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1310660	A0A103Y0W4	53.601	722	194	12	4	588	5	722	0.0	726
Msa1310660	A0A103Y0W4	58.380	358	97	6	265	588	800	1139	1.00e-123	401
Msa1310660	A0A103Y0W4	58.559	222	57	5	1	188	780	1000	4.62e-67	247
Msa1310660	A0A103Y0W4	61.250	80	31	0	189	268	645	724	5.80e-21	109
Msa1310660	A0A103Y0W4	61.395	215	54	4	1	188	331	543	1.93e-70	256
Msa1310660	A0A103Y0W4	64.935	77	27	0	189	265	1062	1138	4.41e-21	109

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa1310660	-0.860824	1.577928e-63	-8.615850e-47
Msa0027580	Msa1310660	0.816257	6.071754e-52	-8.615850e-47
Msa0036370	Msa1310660	0.808285	3.337966e-50	-8.615850e-47
Msa0072170	Msa1310660	0.867608	1.206908e-65	-8.615850e-47
Msa0075340	Msa1310660	0.817016	4.103082e-52	-8.615850e-47
Msa0085720	Msa1310660	0.822209	2.677234e-53	-8.615850e-47
Msa0090030	Msa1310660	0.823808	1.134530e-53	-8.615850e-47
Msa0092380	Msa1310660	0.819074	1.405856e-52	-8.615850e-47
Msa0094010	Msa1310660	0.826168	3.144691e-54	-8.615850e-47
Msa0120370	Msa1310660	-0.803758	2.992306e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1310660	MtrunA17_Chr8g0337471	93.827	324	20	0	265	588	21	344	0.0	632
Msa1310660	MtrunA17_Chr8g0337471	85.106	188	28	0	1	188	1	188	1.09e-111	337
Msa1310660	MtrunA17_Chr8g0337471	88.312	77	9	0	189	265	267	343	2.20e-37	142
Msa1310660	MtrunA17_Chr8g0337491	83.533	334	44	1	265	587	21	354	0.0	579
Msa1310660	MtrunA17_Chr8g0337491	90.452	199	8	1	1	188	1	199	1.16e-126	379
Msa1310660	MtrunA17_Chr8g0337491	59.893	187	33	3	154	298	234	420	1.07e-56	196
Msa1310660	MtrunA17_Chr8g0337491	83.333	54	9	0	1	54	367	420	1.22e-22	100
Msa1310660	MtrunA17_Chr8g0337481	96.491	285	10	0	304	588	1	285	0.0	563
Msa1310660	MtrunA17_Chr8g0337481	86.047	129	18	0	60	188	1	129	4.34e-72	233
Msa1310660	MtrunA17_Chr8g0337481	65.289	121	33	2	154	265	164	284	3.07e-38	142

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1310660	AT5G16970.1	71.341	328	90	3	264	588	19	345	5.74e-171	489
Msa1310660	AT5G16970.1	67.742	186	56	3	6	188	5	189	3.46e-80	256
Msa1310660	AT5G16970.1	71.429	77	22	0	189	265	268	344	1.86e-29	119
Msa1310660	AT5G16990.1	72.393	326	87	3	265	588	19	343	7.63e-170	486
Msa1310660	AT5G16990.1	69.189	185	54	3	6	188	4	187	3.16e-82	261
Msa1310660	AT5G16990.1	67.532	77	25	0	189	265	266	342	5.10e-27	112
Msa1310660	AT5G17000.1	70.948	327	91	3	265	588	20	345	1.03e-167	481
Msa1310660	AT5G17000.1	67.914	187	56	3	6	189	5	190	8.57e-81	258
Msa1310660	AT5G17000.1	68.831	77	24	0	189	265	268	344	1.29e-28	117
Msa1310660	AT3G03080.1	70.948	327	90	3	265	588	26	350	1.54e-164	473

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Find 129 sgRNAs with CRISPR-Local

Find 863 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATTCTCTCCTTTCTT+GGG	0.385342	8_4:-7285901	None:intergenic
AAAGGCCCTGAAGCCTTGGT+TGG	0.572741	8_4:+7286922	Msa1310660:CDS
AAAGGTCCTGCAGCTTTGGT+TGG	0.536064	8_4:+7275758	Msa1310660:CDS
AACCGTGTAATTTAGCAAAT+TGG	0.254826	8_4:-7285963	None:intergenic
AAGCGCTGGAAGTAAAGAGA+AGG	0.678662	8_4:+7285999	Msa1310660:CDS
AAGGAAGGTGATATAGTGTG+GGG	0.603651	8_4:+7285171	Msa1310660:CDS
AAGGAAGGTGATTTAGTGTG+GGG	0.542256	8_4:+7273995	Msa1310660:CDS
AAGTAAACGTCAATGCCTTC+TGG	0.294778	8_4:-7286628	None:intergenic
AAGTATAGTTAATGACTTAA+TGG	0.279576	8_4:-7287201	None:intergenic
AAGTTGACCAACTGCTCCAG+AGG	0.627539	8_4:-7274587	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGTTCATGTCTGATTCTTT+AGG	-	chr8_4:7272409-7272428	None:intergenic	30.0%
ACAATATCATTTGAACCTTG+CGG	-	chr8_4:7272460-7272479	None:intergenic	30.0%
ATGTTGTATGGAATAGAGTT+GGG	+	chr8_4:7272923-7272942	Msa1310660:intron	30.0%
GTGGTAAGAATAAATGAGAT+AGG	+	chr8_4:7273007-7273026	Msa1310660:intron	30.0%
TCTTTAACTCTGTGATTCTT+GGG	+	chr8_4:7273350-7273369	Msa1310660:intron	30.0%
TAGTTAAAGATGGATCATTC+AGG	-	chr8_4:7273464-7273483	None:intergenic	30.0%
GCTCACAAAAAATATTCCAT+AGG	+	chr8_4:7273486-7273505	Msa1310660:intron	30.0%
GAAACAAACAGAATTTCCTA+TGG	-	chr8_4:7273505-7273524	None:intergenic	30.0%
ATTACACAATGCAGATCATA+AGG	+	chr8_4:7273549-7273568	Msa1310660:intron	30.0%
ATTACACAATGCAGATCATA+AGG	-	chr8_4:7273549-7273568	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	7272352	7287277	7272352	ID=Msa1310660;Name=Msa1310660
chr8_4	mRNA	7272352	7287277	7272352	ID=Msa1310660-mRNA-1;Parent=Msa1310660;Name=Msa1310660-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0\|0.55\|0.5\|1\|0.66\|0.5\|10\|246\|588
chr8_4	exon	7272352	7272579	7272352	ID=Msa1310660-mRNA-1:exon:2550;Parent=Msa1310660-mRNA-1
chr8_4	exon	7273941	7274118	7273941	ID=Msa1310660-mRNA-1:exon:2551;Parent=Msa1310660-mRNA-1
chr8_4	exon	7274506	7274661	7274506	ID=Msa1310660-mRNA-1:exon:2552;Parent=Msa1310660-mRNA-1
chr8_4	exon	7275597	7275827	7275597	ID=Msa1310660-mRNA-1:exon:2553;Parent=Msa1310660-mRNA-1
chr8_4	exon	7284308	7284474	7284308	ID=Msa1310660-mRNA-1:exon:2554;Parent=Msa1310660-mRNA-1
chr8_4	exon	7285117	7285294	7285117	ID=Msa1310660-mRNA-1:exon:2555;Parent=Msa1310660-mRNA-1
chr8_4	exon	7285854	7286020	7285854	ID=Msa1310660-mRNA-1:exon:2556;Parent=Msa1310660-mRNA-1
chr8_4	exon	7286414	7286496	7286414	ID=Msa1310660-mRNA-1:exon:2557;Parent=Msa1310660-mRNA-1
chr8_4	exon	7286621	7287049	7286621	ID=Msa1310660-mRNA-1:exon:2558;Parent=Msa1310660-mRNA-1
chr8_4	exon	7287082	7287277	7287082	ID=Msa1310660-mRNA-1:exon:2559;Parent=Msa1310660-mRNA-1
chr8_4	CDS	7272352	7272579	7272352	ID=Msa1310660-mRNA-1:cds;Parent=Msa1310660-mRNA-1
chr8_4	CDS	7273941	7274118	7273941	ID=Msa1310660-mRNA-1:cds;Parent=Msa1310660-mRNA-1
chr8_4	CDS	7274506	7274661	7274506	ID=Msa1310660-mRNA-1:cds;Parent=Msa1310660-mRNA-1
chr8_4	CDS	7275597	7275827	7275597	ID=Msa1310660-mRNA-1:cds;Parent=Msa1310660-mRNA-1
chr8_4	CDS	7284308	7284474	7284308	ID=Msa1310660-mRNA-1:cds;Parent=Msa1310660-mRNA-1
chr8_4	CDS	7285117	7285294	7285117	ID=Msa1310660-mRNA-1:cds;Parent=Msa1310660-mRNA-1
chr8_4	CDS	7285854	7286020	7285854	ID=Msa1310660-mRNA-1:cds;Parent=Msa1310660-mRNA-1
chr8_4	CDS	7286414	7286496	7286414	ID=Msa1310660-mRNA-1:cds;Parent=Msa1310660-mRNA-1
chr8_4	CDS	7286621	7286999	7286621	ID=Msa1310660-mRNA-1:cds;Parent=Msa1310660-mRNA-1
chr8_4	three_prime_UTR	7287000	7287049	7287000	ID=Msa1310660-mRNA-1:three_prime_utr;Parent=Msa1310660-mRNA-1
chr8_4	three_prime_UTR	7287082	7287277	7287082	ID=Msa1310660-mRNA-1:three_prime_utr;Parent=Msa1310660-mRNA-1

Gene Sequence

>Msa1310660

ATGGCGCAAGTAAAGAACAAACAAGTGATTCTGAAACACTATGTGTCTGGTTTTCCTAAAGAATCAGACATGAACATTGTTGAAAGTACCGTAACTCTCAAGCTTCCGCAAGGTTCAAATGATATTGTTCTGCTCAAAAATCTATACTTGTCTTGTGATCCTTACATGAGAAATCTTATGAACAGCCCAGAAAACTGTTTCTATCGTCGTAACTATAGTCCTCAGTCTCCAATAGCTGGCTGTGGTGTGTCTAAAATTGTGGAATCTGGACACAAAGATTATAAGGAAGGTGATTTAGTGTGGGGGTTTACTAATTGGGAAGAGTACAGCTTGATCCCTGCAGCTCAAATACTTTTCAAAATTGAGCACACAGATGTTCCTCTTTCGTACTATACTGGAATTCTTGGTATGCCAGGAATGACCGCTTATGCTGGTTTCTTTGAAGTAGGCTCTCCCAAAAAAGGAGAGAATGTTTTTGTTTCAGCTGCCTCTGGAGCAGTTGGTCAACTTGTTGGACAATTTGCTAAATTGCATGGTTGCTATGTTGTTGGAAGTGCTGGAAGTGTAACAAATTTGGCACATATCATATATAAGCGGATTCGTATGCAAGGTTTCACTGTAGCTAATTATTATCATCTATACACCAAATTCTTAGAGTTTGTCCTGCCTCATATCAGAGAAGGGAAGGTTGTGTATGTAGAAGACATAGCTGAGGGACTTGAGAAAGGTCCTGCAGCTTTGGTTGGCATATTTAGTGGTCACAATGTTGGTAAACAAGTGCTTGTTGTTGCTCAATCAGACCTCAACATTGTTGACAGTACCATTACTCTTAAGATTCCAGAAGGTTCCAACGATGTTGTATTGGTTAAAAATCTTTACTTGTCTTGTGATCCTTACATGAGGACTCTTATGAGTGACCCAAAAGCATCTATCAATCCACGTGCCTATGTTCCTCAATCTCCGATAACTGGTTATGGTGTCTATAAAGTTCTGGAATCTGGACGCAAAGATTATAAGGAAGGTGATATAGTGTGGGGGATAACGAATTGGGAAGAATACAGCTTGATCCCTGCAGCACACATACATTTCAAAATTGAGCATACTGATGTTCCTCTTTCCTACTATACCGGAATTCTTGGTATGCCAGGAATGACTGCTTATTCTGGTTTCTTTGAAGTAGGCTCTCCCAAGAAAGGAGAGAATGTTTTTGTTTCAGCTGCTTCAGGTGCAGTTGGTCAACTTGTTGGCCAATTTGCTAAATTACACGGTTGCTATGTTGTTGGAAGCGCTGGAAGTAAAGAGAAGGTGGATCTGTTGAAGAATAAATTTGGATACGATGAAGCCATTAACTACAAAGAAGAGCAAGACCTCAATGCAGCATTGAAAAGGCACTTTCCAGAAGGCATTGACGTTTACTTTGAGAACGTTGGAGGCAAGACACTTGATGCCGTACTCTTGAACATGAGACTACATGGTCGCATACCTGTGTGTGGAATGATCTCACAGTACAATCTTACTCAACCTGAAGGTGTAACAAATTTAGCACATATCATATATAATCGGATTCGTTTGCAAGGTTTTGTTGTAGTTGATTACTATCATCTATACGGCAAATTCTTGGAGTTTGTCCTGCCTCATATCAGAGAAGGGAAGGTTGTGTATGTGGAAGACATAGCTGAGGGTCTTGAGAAAGGCCCTGAAGCCTTGGTTGGCATATTTAGTGGTAAAAATGTTGGCAAACAAGTGCTTGTTGTTGCTCATGAATGA

Protein sequence

>Msa1310660

MAQVKNKQVILKHYVSGFPKESDMNIVESTVTLKLPQGSNDIVLLKNLYLSCDPYMRNLMNSPENCFYRRNYSPQSPIAGCGVSKIVESGHKDYKEGDLVWGFTNWEEYSLIPAAQILFKIEHTDVPLSYYTGILGMPGMTAYAGFFEVGSPKKGENVFVSAASGAVGQLVGQFAKLHGCYVVGSAGSVTNLAHIIYKRIRMQGFTVANYYHLYTKFLEFVLPHIREGKVVYVEDIAEGLEKGPAALVGIFSGHNVGKQVLVVAQSDLNIVDSTITLKIPEGSNDVVLVKNLYLSCDPYMRTLMSDPKASINPRAYVPQSPITGYGVYKVLESGRKDYKEGDIVWGITNWEEYSLIPAAHIHFKIEHTDVPLSYYTGILGMPGMTAYSGFFEVGSPKKGENVFVSAASGAVGQLVGQFAKLHGCYVVGSAGSKEKVDLLKNKFGYDEAINYKEEQDLNAALKRHFPEGIDVYFENVGGKTLDAVLLNMRLHGRIPVCGMISQYNLTQPEGVTNLAHIIYNRIRLQGFVVVDYYHLYGKFLEFVLPHIREGKVVYVEDIAEGLEKGPEALVGIFSGKNVGKQVLVVAHE*