AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1429550


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1429550 MtrunA17_Chr4g0045901 56.828 227 94 2 11 233 19 245 3.52e-71 219
Msa1429550 MtrunA17_Chr2g0283714 70.253 158 7 1 99 256 16 133 2.87e-70 212
Msa1429550 MtrunA17_Chr4g0041051 47.727 220 109 4 19 234 21 238 8.87e-54 174
Msa1429550 MtrunA17_Chr2g0325521 49.268 205 98 3 29 229 23 225 8.95e-53 171
Msa1429550 MtrunA17_Chr7g0255961 36.957 184 107 3 62 241 70 248 1.22e-30 114
Msa1429550 MtrunA17_Chr3g0113721 39.080 174 87 4 67 227 39 206 1.06e-28 108
Msa1429550 MtrunA17_Chr1g0198801 48.201 139 65 1 63 194 154 292 2.54e-28 110
Msa1429550 MtrunA17_Chr5g0412371 37.857 140 80 2 56 189 34 172 6.09e-23 93.2
Msa1429550 MtrunA17_Chr5g0427361 32.883 222 118 4 63 254 33 253 2.59e-17 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1429550 AT3G56770.1 40.116 172 92 5 62 228 40 205 9.48e-31 114
Msa1429550 AT2G40200.1 38.889 162 91 2 69 224 65 224 4.07e-30 113
Msa1429550 AT1G68810.1 43.605 172 91 4 56 223 164 333 2.18e-28 111
Msa1429550 AT2G41130.1 34.529 223 130 5 17 228 11 228 4.96e-27 105
Msa1429550 AT3G25710.1 44.118 136 73 2 56 189 122 256 1.94e-24 100
Msa1429550 AT4G38070.1 34.959 123 76 2 66 188 91 209 4.48e-17 78.6

Find 45 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAACAATGTCAACTTAT+TGG 0.161618 tig0026517:+62686 Msa1429550:CDS
CTTTACTAGCTGAGGTTATT+AGG 0.226217 tig0026517:+62387 Msa1429550:CDS
ATCAACAAACTCATTGGTTT+TGG 0.255474 tig0026517:+60730 Msa1429550:CDS
CTTAGATCAGTTAGAAGTTC+TGG 0.280659 tig0026517:-62551 None:intergenic
AAGTCCCCTCCAATAATTAA+TGG 0.304566 tig0026517:+60776 Msa1429550:CDS
TAGTAGCTCTGAAGAATCAT+AGG 0.328548 tig0026517:+60831 Msa1429550:CDS
AATGCCACTTCATATAGATT+TGG 0.335342 tig0026517:+60674 Msa1429550:CDS
AAGAGAAGAAACAGAATTAA+TGG 0.350077 tig0026517:+60866 Msa1429550:CDS
TGATAAACTTCAACTACAAT+TGG 0.375139 tig0026517:+62583 Msa1429550:CDS
CCAGTACAGAACTCAGTTCC+TGG 0.377817 tig0026517:-62721 None:intergenic
AAAGTTGAACCATGTGAAAA+TGG 0.387199 tig0026517:+62485 Msa1429550:CDS
AAGGGAAGAAATATCAACTT+TGG 0.396844 tig0026517:+62607 Msa1429550:CDS
AAATATCAACTTTGGGAGAC+AGG 0.404598 tig0026517:+62615 Msa1429550:CDS
TTCACTTCATCATCATCTGT+TGG 0.414183 tig0026517:-62464 None:intergenic
GTTTGTATCAACAAACTCAT+TGG 0.426459 tig0026517:+60724 Msa1429550:CDS
TTCTCTCCATTAATTATTGG+AGG 0.433494 tig0026517:-60782 None:intergenic
AGGGAAGAAATATCAACTTT+GGG 0.445016 tig0026517:+62608 Msa1429550:CDS
AACTTCAACTACAATTGGTA+AGG 0.473092 tig0026517:+62588 Msa1429550:CDS
CCAGGAACTGAGTTCTGTAC+TGG 0.489161 tig0026517:+62721 Msa1429550:CDS
ACTGAGTTCTGTACTGGAAA+GGG 0.489265 tig0026517:+62727 Msa1429550:CDS
CTCTCCATTAATTATTGGAG+GGG 0.498785 tig0026517:-60780 None:intergenic
TATTATACCTTTGGAGAGGA+GGG 0.509382 tig0026517:-60917 None:intergenic
ATTCTAGCTCTGATATTTCG+AGG 0.523502 tig0026517:+60642 Msa1429550:CDS
CAAATGAGACTTCAAGAGGT+GGG 0.540083 tig0026517:+60702 Msa1429550:CDS
TCTCTCCATTAATTATTGGA+GGG 0.544195 tig0026517:-60781 None:intergenic
TTATTATACCTTTGGAGAGG+AGG 0.544800 tig0026517:-60918 None:intergenic
AACTGAGTTCTGTACTGGAA+AGG 0.553572 tig0026517:+62726 Msa1429550:CDS
AGTAGCTCTGAAGAATCATA+GGG 0.557776 tig0026517:+60832 Msa1429550:CDS
CAAAGCAACTTTACTAGCTG+AGG 0.560860 tig0026517:+62379 Msa1429550:CDS
AATGCAGATGAAGCAAGTAA+AGG 0.565648 tig0026517:+62431 Msa1429550:CDS
ACTTGTTCCCTCCTCTCCAA+AGG 0.568287 tig0026517:+60910 Msa1429550:CDS
GGAAAGGGTTTCTACCTCGA+TGG 0.569167 tig0026517:+62742 Msa1429550:CDS
AGCACCAAATCTATATGAAG+TGG 0.573695 tig0026517:-60678 None:intergenic
AATATCAACTTTGGGAGACA+GGG 0.583527 tig0026517:+62616 Msa1429550:CDS
TATTGGAAATAATGTTCACC+AGG 0.592874 tig0026517:+62703 Msa1429550:CDS
TCAAATGAGACTTCAAGAGG+TGG 0.607431 tig0026517:+60701 Msa1429550:CDS
AGATTATTATACCTTTGGAG+AGG 0.615783 tig0026517:-60921 None:intergenic
ACTTCAACTACAATTGGTAA+GGG 0.620942 tig0026517:+62589 Msa1429550:CDS
ATCAACAAGAGCAAAACTCA+TGG 0.629280 tig0026517:-62829 None:intergenic
GTTGAACCATGTGAAAATGG+TGG 0.637388 tig0026517:+62488 Msa1429550:CDS
TGCATCTGTTTGCAAAGACG+TGG 0.639661 tig0026517:-62782 None:intergenic
GAGAGGAGGGAACAAGTGCA+CGG 0.657365 tig0026517:-60904 None:intergenic
GCTTCAAATGAGACTTCAAG+AGG 0.660687 tig0026517:+60698 Msa1429550:CDS
AGAATCATAGGGAAGCAGAG+AGG 0.725124 tig0026517:+60843 Msa1429550:CDS
TCTTAACGAGTAATCCATCG+AGG 0.765808 tig0026517:-62756 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTTTTAAAATATTTTTG+TGG + tig0026517:61039-61058 Msa1429550:intron 10.0%
!!! ATTTTTTAAGGATTATTAAA+TGG - tig0026517:62117-62136 None:intergenic 10.0%
!! ATATAAAAATACGAAAAGAA+AGG - tig0026517:60948-60967 None:intergenic 15.0%
!!! ACTTCGTTATATTTTTTTAA+GGG + tig0026517:60996-61015 Msa1429550:intron 15.0%
!!! ATTGTTATCTGATTTTAAAA+AGG + tig0026517:61153-61172 Msa1429550:intron 15.0%
!!! TTATAATGTTTAACTTTTGT+TGG - tig0026517:61669-61688 None:intergenic 15.0%
!!! TAGTATTAATTTATTTGTTG+TGG + tig0026517:62036-62055 Msa1429550:intron 15.0%
!!! TTTTTCTCTCCATTAATTAT+TGG - tig0026517:60788-60807 None:intergenic 20.0%
!!! CACTTCGTTATATTTTTTTA+AGG + tig0026517:60995-61014 Msa1429550:intron 20.0%
!! AAACTGTAAAAAAGTTCAAT+TGG - tig0026517:61107-61126 None:intergenic 20.0%
!! CTGAAAAAATACATTTACTT+AGG + tig0026517:61349-61368 Msa1429550:intron 20.0%
!! ACTATAATGTAACATCATAT+TGG + tig0026517:61782-61801 Msa1429550:intron 20.0%
!!! TATTAATTTATTTGTTGTGG+TGG + tig0026517:62039-62058 Msa1429550:intron 20.0%
!!! ATTAATTTATTTGTTGTGGT+GGG + tig0026517:62040-62059 Msa1429550:intron 20.0%
!! TTTAAGGATTATTAAATGGT+TGG - tig0026517:62113-62132 None:intergenic 20.0%
!! TATGTACATACTGTTATTAA+TGG + tig0026517:62330-62349 Msa1429550:intron 20.0%
! AAGAGAAGAAACAGAATTAA+TGG + tig0026517:60866-60885 Msa1429550:CDS 25.0%
! AAGGAAGATTATTATACCTT+TGG - tig0026517:60929-60948 None:intergenic 25.0%
!! TATAAACATGTTTTACACGA+TGG + tig0026517:61077-61096 Msa1429550:intron 25.0%
! ATTCCTAATTGCAACTTAAT+TGG - tig0026517:61544-61563 None:intergenic 25.0%
! AAATGAAACAACCTTAGTTT+GGG - tig0026517:61845-61864 None:intergenic 25.0%
! TAAATGAAACAACCTTAGTT+TGG - tig0026517:61846-61865 None:intergenic 25.0%
! TAACATATGATTCTTAGTTG+TGG + tig0026517:61890-61909 Msa1429550:intron 25.0%
! AACATATGATTCTTAGTTGT+GGG + tig0026517:61891-61910 Msa1429550:intron 25.0%
! TGATAAACTTCAACTACAAT+TGG + tig0026517:62583-62602 Msa1429550:CDS 25.0%
! ATTGAACAATGTCAACTTAT+TGG + tig0026517:62686-62705 Msa1429550:CDS 25.0%
! AATGCCACTTCATATAGATT+TGG + tig0026517:60674-60693 Msa1429550:CDS 30.0%
GTTTGTATCAACAAACTCAT+TGG + tig0026517:60724-60743 Msa1429550:CDS 30.0%
!!! ATCAACAAACTCATTGGTTT+TGG + tig0026517:60730-60749 Msa1429550:CDS 30.0%
TCTCTCCATTAATTATTGGA+GGG - tig0026517:60784-60803 None:intergenic 30.0%
TTCTCTCCATTAATTATTGG+AGG - tig0026517:60785-60804 None:intergenic 30.0%
! AGATTATTATACCTTTGGAG+AGG - tig0026517:60924-60943 None:intergenic 30.0%
ACACAATGATCGAATATGAT+TGG - tig0026517:60975-60994 None:intergenic 30.0%
!! TCTGATTTTAAAAAGGCTAG+CGG + tig0026517:61160-61179 Msa1429550:intron 30.0%
ATTGATGGAAATACAAGCTA+TGG + tig0026517:61406-61425 Msa1429550:intron 30.0%
TTGATGGAAATACAAGCTAT+GGG + tig0026517:61407-61426 Msa1429550:intron 30.0%
ATTGAAATTCATGCAGCATT+GGG - tig0026517:61461-61480 None:intergenic 30.0%
! ACACCAATTAAGTTGCAATT+AGG + tig0026517:61538-61557 Msa1429550:intron 30.0%
!! TGTTTAACTTTTGTTGGTGA+TGG - tig0026517:61663-61682 None:intergenic 30.0%
! TTGACTGGTCAAATGTATAT+GGG + tig0026517:61704-61723 Msa1429550:intron 30.0%
! CCTTATATAGTTGACTTTAG+CGG - tig0026517:61732-61751 None:intergenic 30.0%
AAGTCAACTATATAAGGTTG+TGG + tig0026517:61735-61754 Msa1429550:intron 30.0%
! TTTTGCAGATTTATCTTCGA+TGG + tig0026517:61818-61837 Msa1429550:intron 30.0%
!! CAGAAAGAATGATTTGTTGA+GGG - tig0026517:61950-61969 None:intergenic 30.0%
!! TCAGAAAGAATGATTTGTTG+AGG - tig0026517:61951-61970 None:intergenic 30.0%
TCTGATTTCTAATATGCGTT+AGG + tig0026517:62192-62211 Msa1429550:intron 30.0%
CTGATTTCTAATATGCGTTA+GGG + tig0026517:62193-62212 Msa1429550:intron 30.0%
TATGCGTTAGGGTTTAATAT+CGG + tig0026517:62204-62223 Msa1429550:intron 30.0%
AAAGTTGAACCATGTGAAAA+TGG + tig0026517:62485-62504 Msa1429550:CDS 30.0%
AACTTCAACTACAATTGGTA+AGG + tig0026517:62588-62607 Msa1429550:CDS 30.0%
ACTTCAACTACAATTGGTAA+GGG + tig0026517:62589-62608 Msa1429550:CDS 30.0%
AAGGGAAGAAATATCAACTT+TGG + tig0026517:62607-62626 Msa1429550:CDS 30.0%
AGGGAAGAAATATCAACTTT+GGG + tig0026517:62608-62627 Msa1429550:CDS 30.0%
TATTGGAAATAATGTTCACC+AGG + tig0026517:62703-62722 Msa1429550:CDS 30.0%
!! ATTCTAGCTCTGATATTTCG+AGG + tig0026517:60642-60661 Msa1429550:CDS 35.0%
AGCACCAAATCTATATGAAG+TGG - tig0026517:60681-60700 None:intergenic 35.0%
!!! TTGGTTTTGGATAGTGAGAA+AGG + tig0026517:60743-60762 Msa1429550:CDS 35.0%
AAGTCCCCTCCAATAATTAA+TGG + tig0026517:60776-60795 Msa1429550:CDS 35.0%
CTCTCCATTAATTATTGGAG+GGG - tig0026517:60783-60802 None:intergenic 35.0%
!! TAGTAGCTCTGAAGAATCAT+AGG + tig0026517:60831-60850 Msa1429550:CDS 35.0%
!! AGTAGCTCTGAAGAATCATA+GGG + tig0026517:60832-60851 Msa1429550:CDS 35.0%
! TATTATACCTTTGGAGAGGA+GGG - tig0026517:60920-60939 None:intergenic 35.0%
! TTATTATACCTTTGGAGAGG+AGG - tig0026517:60921-60940 None:intergenic 35.0%
ACAGAGACATTTGATATGCT+CGG - tig0026517:61239-61258 None:intergenic 35.0%
AGCTGAAGTTTGAAGATTGA+TGG + tig0026517:61391-61410 Msa1429550:intron 35.0%
CATTGAAATTCATGCAGCAT+TGG - tig0026517:61462-61481 None:intergenic 35.0%
TTAAATTAAGGGTCCTTGAC+TGG + tig0026517:61689-61708 Msa1429550:intron 35.0%
CATATACATTTGACCAGTCA+AGG - tig0026517:61705-61724 None:intergenic 35.0%
! CTTGACTGGTCAAATGTATA+TGG + tig0026517:61703-61722 Msa1429550:intron 35.0%
CCGCTAAAGTCAACTATATA+AGG + tig0026517:61729-61748 Msa1429550:intron 35.0%
TATTAATAGCTGTCTACAGC+TGG + tig0026517:61979-61998 Msa1429550:intron 35.0%
TAACTTTACCTGACCAATGA+AGG + tig0026517:62078-62097 Msa1429550:intron 35.0%
TTGGTTGCTAATTCCTTCAT+TGG - tig0026517:62094-62113 None:intergenic 35.0%
TCATTCAAAACGTAGGTCAT+AGG - tig0026517:62164-62183 None:intergenic 35.0%
!! TGACCTACGTTTTGAATGAA+AGG + tig0026517:62165-62184 Msa1429550:intron 35.0%
!! GTCATTTACGATGTCGATTT+CGG + tig0026517:62284-62303 Msa1429550:intron 35.0%
CTGTTATTAATGGTAGCTGA+TGG + tig0026517:62340-62359 Msa1429550:intron 35.0%
GCTGATGGTATTCTTATAGA+TGG + tig0026517:62355-62374 Msa1429550:intron 35.0%
CTTTACTAGCTGAGGTTATT+AGG + tig0026517:62387-62406 Msa1429550:CDS 35.0%
AATGCAGATGAAGCAAGTAA+AGG + tig0026517:62431-62450 Msa1429550:CDS 35.0%
TTCACTTCATCATCATCTGT+TGG - tig0026517:62467-62486 None:intergenic 35.0%
! AATGATCCACCATTTTCACA+TGG - tig0026517:62497-62516 None:intergenic 35.0%
CTTAGATCAGTTAGAAGTTC+TGG - tig0026517:62554-62573 None:intergenic 35.0%
! AAATATCAACTTTGGGAGAC+AGG + tig0026517:62615-62634 Msa1429550:CDS 35.0%
! AATATCAACTTTGGGAGACA+GGG + tig0026517:62616-62635 Msa1429550:CDS 35.0%
GCTTCAAATGAGACTTCAAG+AGG + tig0026517:60698-60717 Msa1429550:CDS 40.0%
TCAAATGAGACTTCAAGAGG+TGG + tig0026517:60701-60720 Msa1429550:CDS 40.0%
CAAATGAGACTTCAAGAGGT+GGG + tig0026517:60702-60721 Msa1429550:CDS 40.0%
!! ACAATAAGTGCTGAGAAGCA+AGG + tig0026517:61191-61210 Msa1429550:intron 40.0%
CAGAGACATTTGATATGCTC+GGG - tig0026517:61238-61257 None:intergenic 40.0%
ACATCACCAGCTGAATATTC+TGG + tig0026517:61924-61943 Msa1429550:intron 40.0%
TGCTAATTCCTTCATTGGTC+AGG - tig0026517:62089-62108 None:intergenic 40.0%
ACGCCTTTCATTCAAAACGT+AGG - tig0026517:62171-62190 None:intergenic 40.0%
CAAAGCAACTTTACTAGCTG+AGG + tig0026517:62379-62398 Msa1429550:CDS 40.0%
GTTGAACCATGTGAAAATGG+TGG + tig0026517:62488-62507 Msa1429550:CDS 40.0%
AACTGAGTTCTGTACTGGAA+AGG + tig0026517:62726-62745 Msa1429550:CDS 40.0%
ACTGAGTTCTGTACTGGAAA+GGG + tig0026517:62727-62746 Msa1429550:CDS 40.0%
TCTTAACGAGTAATCCATCG+AGG - tig0026517:62759-62778 None:intergenic 40.0%
AGAATCATAGGGAAGCAGAG+AGG + tig0026517:60843-60862 Msa1429550:CDS 45.0%
TACAAGCTATGGGACAGAAC+TGG + tig0026517:61417-61436 Msa1429550:intron 45.0%
TCTTCGATGGTCCCAAACTA+AGG + tig0026517:61831-61850 Msa1429550:intron 45.0%
TGCATCTGTTTGCAAAGACG+TGG - tig0026517:62785-62804 None:intergenic 45.0%
!! TAAACATTATAATTAAATTA+AGG + tig0026517:61677-61696 Msa1429550:intron 5.0%
!! AAACATTATAATTAAATTAA+GGG + tig0026517:61678-61697 Msa1429550:intron 5.0%
!!! TCTTATTTATTTATTTTTTA+AGG - tig0026517:62129-62148 None:intergenic 5.0%
!! AAAAAATAAATAAATAAGAT+AGG + tig0026517:62130-62149 Msa1429550:intron 5.0%
ACTTGTTCCCTCCTCTCCAA+AGG + tig0026517:60910-60929 Msa1429550:CDS 50.0%
! CAGCTGGTGATGTGAAGTGT+GGG - tig0026517:61917-61936 None:intergenic 50.0%
! TCAGCTGGTGATGTGAAGTG+TGG - tig0026517:61918-61937 None:intergenic 50.0%
TGAGGGCCAGAATATTCAGC+TGG - tig0026517:61933-61952 None:intergenic 50.0%
CCAGTACAGAACTCAGTTCC+TGG - tig0026517:62724-62743 None:intergenic 50.0%
CCAGGAACTGAGTTCTGTAC+TGG + tig0026517:62721-62740 Msa1429550:CDS 50.0%
GGAAAGGGTTTCTACCTCGA+TGG + tig0026517:62742-62761 Msa1429550:CDS 50.0%
GAGAGGAGGGAACAAGTGCA+CGG - tig0026517:60907-60926 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0026517 gene 60638 62850 60638 ID=Msa1429550;Name=Msa1429550
tig0026517 mRNA 60638 62850 60638 ID=Msa1429550-mRNA-1;Parent=Msa1429550;Name=Msa1429550-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|1|1|2|0|256
tig0026517 exon 60638 60931 60638 ID=Msa1429550-mRNA-1:exon:6557;Parent=Msa1429550-mRNA-1
tig0026517 exon 62374 62850 62374 ID=Msa1429550-mRNA-1:exon:6558;Parent=Msa1429550-mRNA-1
tig0026517 CDS 60638 60931 60638 ID=Msa1429550-mRNA-1:cds;Parent=Msa1429550-mRNA-1
tig0026517 CDS 62374 62850 62374 ID=Msa1429550-mRNA-1:cds;Parent=Msa1429550-mRNA-1
Gene Sequence

>Msa1429550

ATGGATTCTAGCTCTGATATTTCGAGGATACTATTCAATGCCACTTCATATAGATTTGGTGCTTCAAATGAGACTTCAAGAGGTGGGTTTGTATCAACAAACTCATTGGTTTTGGATAGTGAGAAAGGAGAACTAGTGAAGTCCCCTCCAATAATTAATGGAGAGAAAAAGATTTGTGATGCTAACAAAACTTTAGTAGCTCTGAAGAATCATAGGGAAGCAGAGAGGAAGAGAAGAAACAGAATTAATGGTCATCTTGCTAAACTCCGTGCACTTGTTCCCTCCTCTCCAAAGATGGACAAAGCAACTTTACTAGCTGAGGTTATTAGGCAAGTGAAGCATTTGAAAAAAAATGCAGATGAAGCAAGTAAAGGTTATTCAATTCCAACAGATGATGATGAAGTGAAAGTTGAACCATGTGAAAATGGTGGATCATTTCTTTACAAAGCATCTATCAGCTGCGATTATCGACCAGAACTTCTAACTGATCTAAGACAAACTCTTGATAAACTTCAACTACAATTGGTAAGGGAAGAAATATCAACTTTGGGAGACAGGGTTAAGAATGAATTTGTCTACACATGTTGTAATGTAGACATTTATGATATTGAACAATGTCAACTTATTGGAAATAATGTTCACCAGGAACTGAGTTCTGTACTGGAAAGGGTTTCTACCTCGATGGATTACTCGTTAAGAGCACCACGTCTTTGCAAACAGATGCAGCAGACATCTACTTTGTCATGCAACCATGAGTTTTGCTCTTGTTGA

Protein sequence

>Msa1429550

MDSSSDISRILFNATSYRFGASNETSRGGFVSTNSLVLDSEKGELVKSPPIINGEKKICDANKTLVALKNHREAERKRRNRINGHLAKLRALVPSSPKMDKATLLAEVIRQVKHLKKNADEASKGYSIPTDDDEVKVEPCENGGSFLYKASISCDYRPELLTDLRQTLDKLQLQLVREEISTLGDRVKNEFVYTCCNVDIYDIEQCQLIGNNVHQELSSVLERVSTSMDYSLRAPRLCKQMQQTSTLSCNHEFCSC*