AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024831

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024831.t1 MTR_5g006800 97.290 369 10 0 1 369 1 369 0.0 747
MS.gene024831.t1 MTR_5g006780 92.992 371 24 1 1 369 1 371 0.0 719
MS.gene024831.t1 MTR_5g006750 87.332 371 45 1 1 369 1 371 0.0 683
MS.gene024831.t1 MTR_5g008040 82.337 368 65 0 1 368 3 370 0.0 647
MS.gene024831.t1 MTR_5g006670 84.932 365 49 1 5 369 1 359 0.0 646
MS.gene024831.t1 MTR_5g006740 82.740 365 63 0 5 369 1 365 0.0 645
MS.gene024831.t1 MTR_5g006710 83.747 363 53 1 7 369 7 363 0.0 638
MS.gene024831.t1 MTR_5g006740 79.452 365 58 1 5 369 1 348 0.0 602
MS.gene024831.t1 MTR_5g006720 73.475 377 62 2 4 369 3 352 0.0 563
MS.gene024831.t1 MTR_3g115600 71.111 360 100 1 10 369 8 363 0.0 546
MS.gene024831.t1 MTR_4g125470 53.639 371 157 4 12 368 19 388 2.89e-142 409
MS.gene024831.t1 MTR_5g006730 83.761 117 19 0 1 117 1 117 8.82e-68 210
MS.gene024831.t1 MTR_3g096570 74.359 78 17 1 175 252 8 82 2.70e-31 119
MS.gene024831.t1 MTR_3g096562 67.857 84 24 1 165 248 162 242 9.54e-30 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024831.t1 AT1G76690 77.778 369 82 0 1 369 3 371 0.0 615
MS.gene024831.t1 AT1G76680 77.222 360 82 0 10 369 10 369 0.0 599
MS.gene024831.t1 AT1G76680 72.208 385 82 1 10 369 10 394 0.0 586
MS.gene024831.t1 AT1G09400 70.606 330 91 1 18 347 1 324 2.51e-180 503
MS.gene024831.t1 AT1G09400 71.852 270 70 1 78 347 2 265 6.71e-147 416
MS.gene024831.t1 AT1G17990 71.984 257 67 2 10 266 7 258 1.21e-138 395
MS.gene024831.t1 AT1G18020 71.984 257 67 2 10 266 7 258 1.21e-138 395
MS.gene024831.t1 AT2G06050 51.733 375 162 4 12 368 12 385 1.17e-137 397
MS.gene024831.t1 AT2G06050 51.733 375 162 4 12 368 12 385 1.17e-137 397
MS.gene024831.t1 AT2G06050 51.733 375 162 4 12 368 12 385 1.17e-137 397
MS.gene024831.t1 AT1G17990 68.872 257 67 3 10 266 7 250 4.82e-130 373

Find 107 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAATCTCTTTGGCAAATC+TGG 0.201994 5.4:-2406910 None:intergenic
ATATAAAGTCCCAATTCTTT+AGG 0.204540 5.4:-2406661 None:intergenic
TAAAGAATTGGGACTTTATA+TGG 0.215484 5.4:+2406663 MS.gene024831:CDS
TCACCAACAGTTATCATTCT+TGG 0.270456 5.4:-2406733 None:intergenic
TATAAAGTCCCAATTCTTTA+GGG 0.287263 5.4:-2406660 None:intergenic
CTCCTCTAAACAAGTATAAC+AGG 0.287689 5.4:+2406941 MS.gene024831:CDS
ATTTCATTTCAGAGTTGTTT+TGG 0.300909 5.4:+2405227 MS.gene024831:intron
ATCCAAACACGCCGGGTATA+TGG 0.309625 5.4:+2405382 MS.gene024831:CDS
AAAGGCACTTTCATGGTTGC+AGG 0.311740 5.4:+2406802 MS.gene024831:CDS
GTTAATGCCCTCAATAAATA+TGG 0.323996 5.4:+2406685 MS.gene024831:CDS
TCCCTGTTATACTTGTTTAG+AGG 0.330923 5.4:-2406943 None:intergenic
TACGTTCCTGATCCAGTTCT+AGG 0.338102 5.4:+2406973 MS.gene024831:CDS
CTCATTGGCACAAGGCTGTC+GGG 0.340556 5.4:-2406769 None:intergenic
GTCCATATACCCGGCGTGTT+TGG 0.348909 5.4:-2405384 None:intergenic
TATGAGAAGACCACCTTTAG+AGG 0.360557 5.4:-2405322 None:intergenic
AGACTCCAACCCTAAAGAAT+TGG 0.376017 5.4:+2406651 MS.gene024831:CDS
GTGCAAATTGCAGGATTTGA+TGG 0.377428 5.4:+2406427 MS.gene024831:intron
TTGAATTTGCCCATCTTGTA+AGG 0.380236 5.4:-2405092 None:intergenic
CCTTTGAAGGCCTTTCTCAT+TGG 0.383217 5.4:-2406784 None:intergenic
GGCCTTCAAAGGCACTTTCA+TGG 0.385984 5.4:+2406795 MS.gene024831:CDS
TCATGAACAGCATCTACAAT+AGG 0.392438 5.4:-2405432 None:intergenic
TCAACGCCATAGCTGAAAAC+AGG 0.396930 5.4:+2406848 MS.gene024831:CDS
GCATCAAGTGCAAATCTCTT+TGG 0.407422 5.4:-2406919 None:intergenic
GATATTGGTGCTTGCCCATC+CGG 0.412505 5.4:-2406108 None:intergenic
CGGTTACTTCATTCAAGAAA+AGG 0.415590 5.4:-2407009 None:intergenic
ATACCATTACTTCGAATTTG+TGG 0.416930 5.4:-2406153 None:intergenic
GAACCGTTGTCGGTTTGCTT+TGG 0.420051 5.4:+2406543 MS.gene024831:CDS
CTGAAGTCATTGACAATGTT+CGG 0.421977 5.4:-2406225 None:intergenic
TGCAAATTGCAGGATTTGAT+GGG 0.429144 5.4:+2406428 MS.gene024831:intron
AAAGAATACCATATTTATTG+AGG 0.434046 5.4:-2406693 None:intergenic
TCATCTGTCCTTAGCCGCCT+TGG 0.434930 5.4:-2406198 None:intergenic
GCAAATTCAATCTATCTCAT+AGG 0.435780 5.4:+2405105 MS.gene024831:CDS
AACCATGAAAGTGCCTTTGA+AGG 0.438354 5.4:-2406797 None:intergenic
GTCAGAGACACCGGTAGCTT+CGG 0.447363 5.4:-2405346 None:intergenic
GAGATAGGAACAGAAAGAGT+TGG 0.455231 5.4:+2406589 MS.gene024831:CDS
TCTCATTGGCACAAGGCTGT+CGG 0.469927 5.4:-2406770 None:intergenic
GACTCCAACCCTAAAGAATT+GGG 0.474283 5.4:+2406652 MS.gene024831:CDS
GGAGGTTACGATCGAAATGA+TGG 0.474346 5.4:+2406823 MS.gene024831:CDS
AGGCTCTCTGAGAGTAATAG+AGG 0.476512 5.4:-2405302 None:intergenic
TTCTGTCAAATTTGGCATGT+TGG 0.476753 5.4:+2405471 MS.gene024831:CDS
AGGGCAGACCTAGTTGTATA+TGG 0.479108 5.4:+2406868 MS.gene024831:CDS
TAGTCAGTATAACCTAGAAC+TGG 0.480015 5.4:-2406985 None:intergenic
ACTTCTTACTCCTTACAAGA+TGG 0.486533 5.4:+2405082 MS.gene024831:CDS
AAGAATACCATATTTATTGA+GGG 0.490058 5.4:-2406692 None:intergenic
CTAAAGAGCACGTTGAGGCT+TGG 0.492352 5.4:+2405406 MS.gene024831:CDS
GGTTACTTCATTCAAGAAAA+GGG 0.499808 5.4:-2407008 None:intergenic
CATTCTGCATACTCTGCAAA+AGG 0.500218 5.4:-2406625 None:intergenic
AAGTCCCAATTCTTTAGGGT+TGG 0.500435 5.4:-2406656 None:intergenic
GAAGCTGTTGCAAATGAGAT+AGG 0.501045 5.4:+2406574 MS.gene024831:CDS
TAGAGGATAGCATGAGGTTG+TGG 0.502110 5.4:-2405285 None:intergenic
CAGCCTTGTGCCAATGAGAA+AGG 0.506956 5.4:+2406774 MS.gene024831:CDS
TAAGGAGTAAGAAGTGGAAT+GGG 0.514396 5.4:-2405074 None:intergenic
TGAAAGATAAAGTGAATGAC+AGG 0.515619 5.4:+2406494 MS.gene024831:CDS
GTATAACCTAGAACTGGATC+AGG 0.517171 5.4:-2406979 None:intergenic
GCAAGAAATGCTATTGAAGC+TGG 0.517901 5.4:+2406255 MS.gene024831:CDS
GGCTTGTCTGTGGAAGATAT+TGG 0.523697 5.4:-2406123 None:intergenic
GTTGGAAGAGTTTCTGATGC+TGG 0.526670 5.4:+2405489 MS.gene024831:CDS
AAACCACAAATTCGAAGTAA+TGG 0.528619 5.4:+2406150 MS.gene024831:CDS
GTAAGGAGTAAGAAGTGGAA+TGG 0.528759 5.4:-2405075 None:intergenic
GATCCCTTGAGAACCGTTGT+CGG 0.529555 5.4:+2406533 MS.gene024831:CDS
TTTGATGGGGTTGAAATCCA+TGG 0.529668 5.4:+2406442 MS.gene024831:CDS
CTTTCATGAATTGTTCAAGT+AGG 0.530510 5.4:-2406477 None:intergenic
GTTGAAATCCATGGAGCTCA+TGG 0.532007 5.4:+2406451 MS.gene024831:CDS
CAAACCGACAACGGTTCTCA+AGG 0.533263 5.4:-2406537 None:intergenic
TCTGTCCTTAGCCGCCTTGG+TGG 0.535739 5.4:-2406195 None:intergenic
TTGAGCAGTGTCAGAGACAC+CGG 0.539225 5.4:-2405355 None:intergenic
CTTCTTACTCCTTACAAGAT+GGG 0.539764 5.4:+2405083 MS.gene024831:CDS
TCACACCACCAAGGCGGCTA+AGG 0.540861 5.4:+2406190 MS.gene024831:CDS
ATTCTCAACAGTTTATCAGC+CGG 0.545602 5.4:+2406089 MS.gene024831:intron
CAACAGTTTATCAGCCGGAT+GGG 0.548494 5.4:+2406094 MS.gene024831:intron
AATGACAGGACAGATGAGTA+TGG 0.553924 5.4:+2406508 MS.gene024831:CDS
TCAACAGTTTATCAGCCGGA+TGG 0.554883 5.4:+2406093 MS.gene024831:intron
TACTCTCAGAGAGCCTCTAA+AGG 0.554945 5.4:+2405309 MS.gene024831:CDS
AGGCCTTTCTCATTGGCACA+AGG 0.555706 5.4:-2406777 None:intergenic
CTTCTCATAGCCGAAGCTAC+CGG 0.560076 5.4:+2405336 MS.gene024831:CDS
CAAGGCTATCCAAACACGCC+GGG 0.568991 5.4:+2405375 MS.gene024831:CDS
CCAATGAGAAAGGCCTTCAA+AGG 0.575485 5.4:+2406784 MS.gene024831:CDS
TGGTATTCTTTATTGTCACA+TGG 0.580029 5.4:+2406705 MS.gene024831:CDS
TCATTGGCACAAGGCTGTCG+GGG 0.584370 5.4:-2406768 None:intergenic
GGCACTTTCATGGTTGCAGG+AGG 0.593746 5.4:+2406805 MS.gene024831:CDS
GTAGGTAGCCATGAGCTCCA+TGG 0.595976 5.4:-2406459 None:intergenic
TTTGCAGAGTATGCAGAATG+TGG 0.596323 5.4:+2406628 MS.gene024831:CDS
CTCACAAGACAAAGATCCTA+CGG 0.598712 5.4:+2405255 MS.gene024831:CDS
ACTTCAGTACAGGAAATGGC+TGG 0.601525 5.4:+2405035 None:intergenic
ATCTTGTAAGGAGTAAGAAG+TGG 0.605352 5.4:-2405080 None:intergenic
GCAAATTGCAGGATTTGATG+GGG 0.608055 5.4:+2406429 MS.gene024831:intron
CTTCCAAAGCAAACCGACAA+CGG 0.620487 5.4:-2406546 None:intergenic
GGTGTCTCTGACACTGCTCA+AGG 0.621150 5.4:+2405357 MS.gene024831:CDS
ACAAACGACCATATACAACT+AGG 0.621826 5.4:-2406876 None:intergenic
AAACCGACAACGGTTCTCAA+GGG 0.626011 5.4:-2406536 None:intergenic
TGCTCTTTAGTCCATATACC+CGG 0.626306 5.4:-2405393 None:intergenic
CAACGCCATAGCTGAAAACA+GGG 0.626950 5.4:+2406849 MS.gene024831:CDS
GTAGATGCTGTTCATGACAA+AGG 0.628826 5.4:+2405438 MS.gene024831:CDS
GAGGTTACGATCGAAATGAT+GGG 0.630066 5.4:+2406824 MS.gene024831:CDS
TATTCTTTATTGTCACATGG+TGG 0.639708 5.4:+2406708 MS.gene024831:CDS
TAGGATCTTTGTCTTGTGAG+TGG 0.640382 5.4:-2405252 None:intergenic
TCCTCTAAACAAGTATAACA+GGG 0.645269 5.4:+2406942 MS.gene024831:CDS
GACAGGACAGATGAGTATGG+TGG 0.649651 5.4:+2406511 MS.gene024831:CDS
TCAAGGCTATCCAAACACGC+CGG 0.650428 5.4:+2405374 MS.gene024831:CDS
AGGTTGTGGAACATTGCCGT+AGG 0.656729 5.4:-2405271 None:intergenic
TCTCAGAGAGCCTCTAAAGG+TGG 0.663690 5.4:+2405312 MS.gene024831:CDS
GAGTAATAGAGGATAGCATG+AGG 0.689366 5.4:-2405291 None:intergenic
GAACCAAGAATGATAACTGT+TGG 0.691135 5.4:+2406730 MS.gene024831:CDS
TTGCAAAATTCACACCACCA+AGG 0.719184 5.4:+2406181 MS.gene024831:CDS
ATGGACTAAAGAGCACGTTG+AGG 0.722206 5.4:+2405401 MS.gene024831:CDS
CAAAATTCACACCACCAAGG+CGG 0.741166 5.4:+2406184 MS.gene024831:CDS
GATGCTGTTCATGACAAAGG+CGG 0.776205 5.4:+2405441 MS.gene024831:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATTGTTTACAATAAAA+GGG + chr5.4:2405934-2405953 MS.gene024831:intron 10.0%
!! CTAATATTGTTTACAATAAA+AGG + chr5.4:2405933-2405952 MS.gene024831:intron 15.0%
!! AAAGAATACCATATTTATTG+AGG - chr5.4:2406696-2406715 None:intergenic 20.0%
!! AACATGACATAAAACATAAA+CGG + chr5.4:2405664-2405683 MS.gene024831:intron 20.0%
!! AAGAATACCATATTTATTGA+GGG - chr5.4:2406695-2406714 None:intergenic 20.0%
!! AATATGATATGATATGTTCT+AGG + chr5.4:2405787-2405806 MS.gene024831:intron 20.0%
!! AATATGGAATGATAAATGAA+TGG - chr5.4:2405148-2405167 None:intergenic 20.0%
!! AATTATGTAACATACTTAGT+GGG + chr5.4:2406404-2406423 MS.gene024831:intron 20.0%
!! TTGTTATCGAGTATTATTAA+GGG + chr5.4:2405978-2405997 MS.gene024831:intron 20.0%
! ACATGACATAAAACATAAAC+GGG + chr5.4:2405665-2405684 MS.gene024831:intron 25.0%
! ATAAACTGTTGAGAATAACA+TGG - chr5.4:2406085-2406104 None:intergenic 25.0%
! ATATAAAGTCCCAATTCTTT+AGG - chr5.4:2406664-2406683 None:intergenic 25.0%
! ATCACACATAAATGTTCTAA+TGG + chr5.4:2406026-2406045 MS.gene024831:intron 25.0%
! ATTATGTAACATACTTAGTG+GGG + chr5.4:2406405-2406424 MS.gene024831:intron 25.0%
! CAATTATGTAACATACTTAG+TGG + chr5.4:2406403-2406422 MS.gene024831:intron 25.0%
! CACTAAGTATGTTACATAAT+TGG - chr5.4:2406405-2406424 None:intergenic 25.0%
! GTTGTTATCGAGTATTATTA+AGG + chr5.4:2405977-2405996 MS.gene024831:intron 25.0%
! TAAAGAATTGGGACTTTATA+TGG + chr5.4:2406663-2406682 MS.gene024831:CDS 25.0%
! TATAAAGTCCCAATTCTTTA+GGG - chr5.4:2406663-2406682 None:intergenic 25.0%
! TGAGCAATTGAAATTGAATA+TGG - chr5.4:2405164-2405183 None:intergenic 25.0%
! TGTTATCGAGTATTATTAAG+GGG + chr5.4:2405979-2405998 MS.gene024831:intron 25.0%
!! ATTTCATTTCAGAGTTGTTT+TGG + chr5.4:2405227-2405246 MS.gene024831:intron 25.0%
!! CAAAAGTATATTTGTACAAC+AGG - chr5.4:2405894-2405913 None:intergenic 25.0%
!! CTGTTGTACAAATATACTTT+TGG + chr5.4:2405892-2405911 MS.gene024831:intron 25.0%
!! TTACAATAAAAGGGTATGAT+TGG + chr5.4:2405943-2405962 MS.gene024831:intron 25.0%
!!! GAGATTTTTGTTACTTTAGA+TGG + chr5.4:2406365-2406384 MS.gene024831:intron 25.0%
!!! TACATTTTGACTAAAGACAT+AGG - chr5.4:2405729-2405748 None:intergenic 25.0%
AAACCACAAATTCGAAGTAA+TGG + chr5.4:2406150-2406169 MS.gene024831:CDS 30.0%
ATACCATTACTTCGAATTTG+TGG - chr5.4:2406156-2406175 None:intergenic 30.0%
CATGACATAAAACATAAACG+GGG + chr5.4:2405666-2405685 MS.gene024831:intron 30.0%
CTTTAGTCAAAATGTAACTG+CGG + chr5.4:2405733-2405752 MS.gene024831:intron 30.0%
CTTTCATGAATTGTTCAAGT+AGG - chr5.4:2406480-2406499 None:intergenic 30.0%
GAGTAAATAACACAAACACA+TGG - chr5.4:2405207-2405226 None:intergenic 30.0%
GCAAATTCAATCTATCTCAT+AGG + chr5.4:2405105-2405124 MS.gene024831:CDS 30.0%
GTTAATGCCCTCAATAAATA+TGG + chr5.4:2406685-2406704 MS.gene024831:CDS 30.0%
GTTATCGAGTATTATTAAGG+GGG + chr5.4:2405980-2405999 MS.gene024831:intron 30.0%
TATTCTTTATTGTCACATGG+TGG + chr5.4:2406708-2406727 MS.gene024831:CDS 30.0%
TCCTCTAAACAAGTATAACA+GGG + chr5.4:2406942-2406961 MS.gene024831:CDS 30.0%
TGAAAGATAAAGTGAATGAC+AGG + chr5.4:2406494-2406513 MS.gene024831:CDS 30.0%
TGGTATTCTTTATTGTCACA+TGG + chr5.4:2406705-2406724 MS.gene024831:CDS 30.0%
! GTGTGTTTTTCTGTCAAATT+TGG + chr5.4:2405463-2405482 MS.gene024831:CDS 30.0%
!! GGCATTTTAATCAGAGTAAT+AGG - chr5.4:2405708-2405727 None:intergenic 30.0%
!! TAGGAGTTATTCGTTTTTCT+TGG + chr5.4:2405619-2405638 MS.gene024831:intron 30.0%
AATGTAACTGCGGAAAGAAT+TGG + chr5.4:2405743-2405762 MS.gene024831:intron 35.0%
ACAAACGACCATATACAACT+AGG - chr5.4:2406879-2406898 None:intergenic 35.0%
ACCTCAAATCAATACTTGTG+TGG - chr5.4:2405570-2405589 None:intergenic 35.0%
ACTGAGTGAATTATGTGACT+AGG + chr5.4:2405600-2405619 MS.gene024831:intron 35.0%
ACTTCTTACTCCTTACAAGA+TGG + chr5.4:2405082-2405101 MS.gene024831:CDS 35.0%
ATCTTGTAAGGAGTAAGAAG+TGG - chr5.4:2405083-2405102 None:intergenic 35.0%
ATTCTCAACAGTTTATCAGC+CGG + chr5.4:2406089-2406108 MS.gene024831:intron 35.0%
CTCCTCTAAACAAGTATAAC+AGG + chr5.4:2406941-2406960 MS.gene024831:CDS 35.0%
CTGAAGTCATTGACAATGTT+CGG - chr5.4:2406228-2406247 None:intergenic 35.0%
CTTCTTACTCCTTACAAGAT+GGG + chr5.4:2405083-2405102 MS.gene024831:CDS 35.0%
GAACCAAGAATGATAACTGT+TGG + chr5.4:2406730-2406749 MS.gene024831:CDS 35.0%
TAAGGAGTAAGAAGTGGAAT+GGG - chr5.4:2405077-2405096 None:intergenic 35.0%
TAGTCAGTATAACCTAGAAC+TGG - chr5.4:2406988-2407007 None:intergenic 35.0%
TCACCAACAGTTATCATTCT+TGG - chr5.4:2406736-2406755 None:intergenic 35.0%
TCATGAACAGCATCTACAAT+AGG - chr5.4:2405435-2405454 None:intergenic 35.0%
TCCCTGTTATACTTGTTTAG+AGG - chr5.4:2406946-2406965 None:intergenic 35.0%
TGCAAATTGCAGGATTTGAT+GGG + chr5.4:2406428-2406447 MS.gene024831:intron 35.0%
TTCTGTCAAATTTGGCATGT+TGG + chr5.4:2405471-2405490 MS.gene024831:CDS 35.0%
TTGAATTTGCCCATCTTGTA+AGG - chr5.4:2405095-2405114 None:intergenic 35.0%
! CATTCAGTTTTCCTCACTTA+AGG - chr5.4:2405833-2405852 None:intergenic 35.0%
!! ATGATATGTTCTAGGACAGT+AGG + chr5.4:2405795-2405814 MS.gene024831:intron 35.0%
!! ATTGATTTGAGGTTTCCTTG+TGG + chr5.4:2405577-2405596 MS.gene024831:intron 35.0%
!! TTGTTACTTTAGATGGCACT+AGG + chr5.4:2406372-2406391 MS.gene024831:intron 35.0%
!!! CTTCGAATTTGTGGTTTTAG+TGG - chr5.4:2406147-2406166 None:intergenic 35.0%
AATGACAGGACAGATGAGTA+TGG + chr5.4:2406508-2406527 MS.gene024831:CDS 40.0%
AGACTCCAACCCTAAAGAAT+TGG + chr5.4:2406651-2406670 MS.gene024831:CDS 40.0%
CACACATGTTACCTTAAGTG+AGG + chr5.4:2405819-2405838 MS.gene024831:intron 40.0%
CATAATTCACTCAGTCCACA+AGG - chr5.4:2405595-2405614 None:intergenic 40.0%
CATTCTGCATACTCTGCAAA+AGG - chr5.4:2406628-2406647 None:intergenic 40.0%
CTCACAAGACAAAGATCCTA+CGG + chr5.4:2405255-2405274 MS.gene024831:CDS 40.0%
GAAGCTGTTGCAAATGAGAT+AGG + chr5.4:2406574-2406593 MS.gene024831:CDS 40.0%
GACTCCAACCCTAAAGAATT+GGG + chr5.4:2406652-2406671 MS.gene024831:CDS 40.0%
GAGATAGGAACAGAAAGAGT+TGG + chr5.4:2406589-2406608 MS.gene024831:CDS 40.0%
GAGGTTACGATCGAAATGAT+GGG + chr5.4:2406824-2406843 MS.gene024831:CDS 40.0%
GAGTAATAGAGGATAGCATG+AGG - chr5.4:2405294-2405313 None:intergenic 40.0%
GCAAATCTCTTTGGCAAATC+TGG - chr5.4:2406913-2406932 None:intergenic 40.0%
GCAAATTGCAGGATTTGATG+GGG + chr5.4:2406429-2406448 MS.gene024831:intron 40.0%
GCAAGAAATGCTATTGAAGC+TGG + chr5.4:2406255-2406274 MS.gene024831:CDS 40.0%
GCATCAAGTGCAAATCTCTT+TGG - chr5.4:2406922-2406941 None:intergenic 40.0%
GTAAGGAGTAAGAAGTGGAA+TGG - chr5.4:2405078-2405097 None:intergenic 40.0%
GTAGATGCTGTTCATGACAA+AGG + chr5.4:2405438-2405457 MS.gene024831:CDS 40.0%
GTATAACCTAGAACTGGATC+AGG - chr5.4:2406982-2407001 None:intergenic 40.0%
GTGCAAATTGCAGGATTTGA+TGG + chr5.4:2406427-2406446 MS.gene024831:intron 40.0%
TAGGATCTTTGTCTTGTGAG+TGG - chr5.4:2405255-2405274 None:intergenic 40.0%
TATGAGAAGACCACCTTTAG+AGG - chr5.4:2405325-2405344 None:intergenic 40.0%
TGCTCTTTAGTCCATATACC+CGG - chr5.4:2405396-2405415 None:intergenic 40.0%
TTGCAAAATTCACACCACCA+AGG + chr5.4:2406181-2406200 MS.gene024831:CDS 40.0%
TTTGATGGGGTTGAAATCCA+TGG + chr5.4:2406442-2406461 MS.gene024831:CDS 40.0%
TTTGCAGAGTATGCAGAATG+TGG + chr5.4:2406628-2406647 MS.gene024831:CDS 40.0%
!! AACCATGAAAGTGCCTTTGA+AGG - chr5.4:2406800-2406819 None:intergenic 40.0%
!! AAGTCCCAATTCTTTAGGGT+TGG - chr5.4:2406659-2406678 None:intergenic 40.0%
!! GCCACACAAGTATTGATTTG+AGG + chr5.4:2405566-2405585 MS.gene024831:intron 40.0%
AAACCGACAACGGTTCTCAA+GGG - chr5.4:2406539-2406558 None:intergenic 45.0%
AATACTTGTGTGGCAACTCG+CGG - chr5.4:2405560-2405579 None:intergenic 45.0%
AGGGCAGACCTAGTTGTATA+TGG + chr5.4:2406868-2406887 MS.gene024831:CDS 45.0%
CAAAATTCACACCACCAAGG+CGG + chr5.4:2406184-2406203 MS.gene024831:CDS 45.0%
CAACAGTTTATCAGCCGGAT+GGG + chr5.4:2406094-2406113 MS.gene024831:intron 45.0%
CAACGCCATAGCTGAAAACA+GGG + chr5.4:2406849-2406868 MS.gene024831:CDS 45.0%
CCAATGAGAAAGGCCTTCAA+AGG + chr5.4:2406784-2406803 MS.gene024831:CDS 45.0%
CTTCCAAAGCAAACCGACAA+CGG - chr5.4:2406549-2406568 None:intergenic 45.0%
GATGCTGTTCATGACAAAGG+CGG + chr5.4:2405441-2405460 MS.gene024831:CDS 45.0%
GGAGGTTACGATCGAAATGA+TGG + chr5.4:2406823-2406842 MS.gene024831:CDS 45.0%
GGCTTGTCTGTGGAAGATAT+TGG - chr5.4:2406126-2406145 None:intergenic 45.0%
GTTGAAATCCATGGAGCTCA+TGG + chr5.4:2406451-2406470 MS.gene024831:CDS 45.0%
GTTGGAAGAGTTTCTGATGC+TGG + chr5.4:2405489-2405508 MS.gene024831:CDS 45.0%
TACGTTCCTGATCCAGTTCT+AGG + chr5.4:2406973-2406992 MS.gene024831:CDS 45.0%
TACTCTCAGAGAGCCTCTAA+AGG + chr5.4:2405309-2405328 MS.gene024831:CDS 45.0%
TAGAGGATAGCATGAGGTTG+TGG - chr5.4:2405288-2405307 None:intergenic 45.0%
TCAACAGTTTATCAGCCGGA+TGG + chr5.4:2406093-2406112 MS.gene024831:intron 45.0%
TCAACGCCATAGCTGAAAAC+AGG + chr5.4:2406848-2406867 MS.gene024831:CDS 45.0%
! AGGCTCTCTGAGAGTAATAG+AGG - chr5.4:2405305-2405324 None:intergenic 45.0%
! ATGGACTAAAGAGCACGTTG+AGG + chr5.4:2405401-2405420 MS.gene024831:CDS 45.0%
! CCTTTGAAGGCCTTTCTCAT+TGG - chr5.4:2406787-2406806 None:intergenic 45.0%
!! AAAGGCACTTTCATGGTTGC+AGG + chr5.4:2406802-2406821 MS.gene024831:CDS 45.0%
!! CTCGCGGTACTTTTGTTTAC+TGG - chr5.4:2405544-2405563 None:intergenic 45.0%
!!! TGGTTTTAGTGGCTTGTCTG+TGG - chr5.4:2406136-2406155 None:intergenic 45.0%
AGGCCTTTCTCATTGGCACA+AGG - chr5.4:2406780-2406799 None:intergenic 50.0%
AGGTTGTGGAACATTGCCGT+AGG - chr5.4:2405274-2405293 None:intergenic 50.0%
ATCCAAACACGCCGGGTATA+TGG + chr5.4:2405382-2405401 MS.gene024831:CDS 50.0%
CAAACCGACAACGGTTCTCA+AGG - chr5.4:2406540-2406559 None:intergenic 50.0%
CTTAGTGGGGTGCAAATTGC+AGG + chr5.4:2406418-2406437 MS.gene024831:intron 50.0%
CTTCTCATAGCCGAAGCTAC+CGG + chr5.4:2405336-2405355 MS.gene024831:CDS 50.0%
GACAGGACAGATGAGTATGG+TGG + chr5.4:2406511-2406530 MS.gene024831:CDS 50.0%
GATCCCTTGAGAACCGTTGT+CGG + chr5.4:2406533-2406552 MS.gene024831:CDS 50.0%
TCAAGGCTATCCAAACACGC+CGG + chr5.4:2405374-2405393 MS.gene024831:CDS 50.0%
TCTCATTGGCACAAGGCTGT+CGG - chr5.4:2406773-2406792 None:intergenic 50.0%
TTGAGCAGTGTCAGAGACAC+CGG - chr5.4:2405358-2405377 None:intergenic 50.0%
! CAGCCTTGTGCCAATGAGAA+AGG + chr5.4:2406774-2406793 MS.gene024831:CDS 50.0%
! CTAAAGAGCACGTTGAGGCT+TGG + chr5.4:2405406-2405425 MS.gene024831:CDS 50.0%
! GATATTGGTGCTTGCCCATC+CGG - chr5.4:2406111-2406130 None:intergenic 50.0%
! GTCTGCCCTGTTTTCAGCTA+TGG - chr5.4:2406857-2406876 None:intergenic 50.0%
! TCTCAGAGAGCCTCTAAAGG+TGG + chr5.4:2405312-2405331 MS.gene024831:CDS 50.0%
!! GAACCGTTGTCGGTTTGCTT+TGG + chr5.4:2406543-2406562 MS.gene024831:CDS 50.0%
!! GGCCTTCAAAGGCACTTTCA+TGG + chr5.4:2406795-2406814 MS.gene024831:CDS 50.0%
CAAGGCTATCCAAACACGCC+GGG + chr5.4:2405375-2405394 MS.gene024831:CDS 55.0%
CTCATTGGCACAAGGCTGTC+GGG - chr5.4:2406772-2406791 None:intergenic 55.0%
GGCACTTTCATGGTTGCAGG+AGG + chr5.4:2406805-2406824 MS.gene024831:CDS 55.0%
GGTGTCTCTGACACTGCTCA+AGG + chr5.4:2405357-2405376 MS.gene024831:CDS 55.0%
GTAGGTAGCCATGAGCTCCA+TGG - chr5.4:2406462-2406481 None:intergenic 55.0%
GTCAGAGACACCGGTAGCTT+CGG - chr5.4:2405349-2405368 None:intergenic 55.0%
GTCCATATACCCGGCGTGTT+TGG - chr5.4:2405387-2405406 None:intergenic 55.0%
TCATCTGTCCTTAGCCGCCT+TGG - chr5.4:2406201-2406220 None:intergenic 55.0%
TCATTGGCACAAGGCTGTCG+GGG - chr5.4:2406771-2406790 None:intergenic 55.0%
TCACACCACCAAGGCGGCTA+AGG + chr5.4:2406190-2406209 MS.gene024831:CDS 60.0%
TCTGTCCTTAGCCGCCTTGG+TGG - chr5.4:2406198-2406217 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 2405050 2407023 2405050 ID=MS.gene024831
chr5.4 mRNA 2405050 2407023 2405050 ID=MS.gene024831.t1;Parent=MS.gene024831
chr5.4 exon 2405050 2405126 2405050 ID=MS.gene024831.t1.exon1;Parent=MS.gene024831.t1
chr5.4 CDS 2405050 2405126 2405050 ID=cds.MS.gene024831.t1;Parent=MS.gene024831.t1
chr5.4 exon 2405239 2405510 2405239 ID=MS.gene024831.t1.exon2;Parent=MS.gene024831.t1
chr5.4 CDS 2405239 2405510 2405239 ID=cds.MS.gene024831.t1;Parent=MS.gene024831.t1
chr5.4 exon 2406100 2406276 2406100 ID=MS.gene024831.t1.exon3;Parent=MS.gene024831.t1
chr5.4 CDS 2406100 2406276 2406100 ID=cds.MS.gene024831.t1;Parent=MS.gene024831.t1
chr5.4 exon 2406440 2407023 2406440 ID=MS.gene024831.t1.exon4;Parent=MS.gene024831.t1
chr5.4 CDS 2406440 2407023 2406440 ID=cds.MS.gene024831.t1;Parent=MS.gene024831.t1
Gene Sequence

>MS.gene024831

ATGGCTGGTGTTGTTGAATCTGATCCCATTCCACTTCTTACTCCTTACAAGATGGGCAAATTCAATCTATCTCATAGGTATTTTTATTATCTTTTCCATTCATTTATCATTCCATATTCAATTTCAATTGCTCAAATATTCACACACACACACACCATGTGTTTGTGTTATTTACTCATTTCATTTCAGAGTTGTTTTGGCACCACTCACAAGACAAAGATCCTACGGCAATGTTCCACAACCTCATGCTATCCTCTATTACTCTCAGAGAGCCTCTAAAGGTGGTCTTCTCATAGCCGAAGCTACCGGTGTCTCTGACACTGCTCAAGGCTATCCAAACACGCCGGGTATATGGACTAAAGAGCACGTTGAGGCTTGGAAACCTATTGTAGATGCTGTTCATGACAAAGGCGGTGTGTTTTTCTGTCAAATTTGGCATGTTGGAAGAGTTTCTGATGCTGGTATGCTCTATTTCATCAAATTATCATGAGCCAGTAAACAAAAGTACCGCGAGTTGCCACACAAGTATTGATTTGAGGTTTCCTTGTGGACTGAGTGAATTATGTGACTAGGAGTTATTCGTTTTTCTTGGCATACAATCGTGATATAGATTTAACATGACATAAAACATAAACGGGGACTTTGTTTATTTGAACCTATTACTCTGATTAAAATGCCTATGTCTTTAGTCAAAATGTAACTGCGGAAAGAATTGGTGCAAAATGCTATTAACAGTAAATATGATATGATATGTTCTAGGACAGTAGGACACACATGTTACCTTAAGTGAGGAAAACTGAATGATTAATTTGTAAATGTGTCATGTTGCATTTGTTGTCAACCTGTTGTACAAATATACTTTTGGCATCTATTAAATAAAAGTCTAATATTGTTTACAATAAAAGGGTATGATTGGAGTGAAAGATTGTTGTTATCGAGTATTATTAAGGGGGAAACTGCATTCTGATTTTTAGCTATCACACATAAATGTTCTAATGGCTGTGATGTACAAGCTCATTGTATGCTCATTTTCCATGTTATTCTCAACAGTTTATCAGCCGGATGGGCAAGCACCAATATCTTCCACAGACAAGCCACTAAAACCACAAATTCGAAGTAATGGTATTGATGTTGCAAAATTCACACCACCAAGGCGGCTAAGGACAGATGAAATTCCGAACATTGTCAATGACTTCAGACTCGCTGCAAGAAATGCTATTGAAGCTGGTAACTTTACTAATCTTTACAAATGTCTCAAAAATTAAAAAAATTCTTTAATGTATCATAATACAATGTATTATGTATGTGAGACGTAGAGATTTTTGTTACTTTAGATGGCACTAGGACATTTTCCAATTATGTAACATACTTAGTGGGGTGCAAATTGCAGGATTTGATGGGGTTGAAATCCATGGAGCTCATGGCTACCTACTTGAACAATTCATGAAAGATAAAGTGAATGACAGGACAGATGAGTATGGTGGATCCCTTGAGAACCGTTGTCGGTTTGCTTTGGAAGTTGTTGAAGCTGTTGCAAATGAGATAGGAACAGAAAGAGTTGGAATAAGATTATCACCTTTTGCAGAGTATGCAGAATGTGGAGACTCCAACCCTAAAGAATTGGGACTTTATATGGTTAATGCCCTCAATAAATATGGTATTCTTTATTGTCACATGGTGGAACCAAGAATGATAACTGTTGGTGAAAAAACTGAATGCCCCGACAGCCTTGTGCCAATGAGAAAGGCCTTCAAAGGCACTTTCATGGTTGCAGGAGGTTACGATCGAAATGATGGGATCAACGCCATAGCTGAAAACAGGGCAGACCTAGTTGTATATGGTCGTTTGTTCTTAGCTAATCCAGATTTGCCAAAGAGATTTGCACTTGATGCTCCTCTAAACAAGTATAACAGGGAGACATTTTACGTTCCTGATCCAGTTCTAGGTTATACTGACTACCCTTTTCTTGAATGA
Protein sequence

>MS.gene024831.t1

MAGVVESDPIPLLTPYKMGKFNLSHRVVLAPLTRQRSYGNVPQPHAILYYSQRASKGGLLIAEATGVSDTAQGYPNTPGIWTKEHVEAWKPIVDAVHDKGGVFFCQIWHVGRVSDAVYQPDGQAPISSTDKPLKPQIRSNGIDVAKFTPPRRLRTDEIPNIVNDFRLAARNAIEAGFDGVEIHGAHGYLLEQFMKDKVNDRTDEYGGSLENRCRFALEVVEAVANEIGTERVGIRLSPFAEYAECGDSNPKELGLYMVNALNKYGILYCHMVEPRMITVGEKTECPDSLVPMRKAFKGTFMVAGGYDRNDGINAIAENRADLVVYGRLFLANPDLPKRFALDAPLNKYNRETFYVPDPVLGYTDYPFLE