AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81774


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81774.t1 MTR_1g058540 95.918 196 8 0 1 196 1 196 2.61e-120 338
MS.gene81774.t1 MTR_1g058570 77.041 196 43 1 1 196 1 194 3.19e-93 270
MS.gene81774.t1 MTR_1g058560 80.102 196 38 1 1 196 1 195 1.15e-91 266
MS.gene81774.t1 MTR_2g086740 52.857 210 83 1 2 195 10 219 2.30e-59 185
MS.gene81774.t1 MTR_7g011090 45.263 190 100 1 7 196 1 186 3.33e-52 166
MS.gene81774.t1 MTR_4g081880 42.197 173 100 0 23 195 14 186 2.16e-47 154
MS.gene81774.t1 MTR_5g041900 40.722 194 102 2 4 196 5 186 1.46e-41 139
MS.gene81774.t1 MTR_2g086740 49.645 141 55 1 2 126 10 150 6.56e-34 118
MS.gene81774.t1 MTR_0004s0210 34.615 156 87 4 37 189 7 150 4.06e-18 77.8
MS.gene81774.t1 MTR_0097s0070 34.167 120 73 3 73 189 69 185 1.64e-12 63.9
MS.gene81774.t1 MTR_3g064310 27.564 156 96 3 42 193 49 191 4.83e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81774.t1 AT2G36100 54.950 202 84 2 2 196 5 206 3.24e-68 207
MS.gene81774.t1 AT3G11550 60.500 200 74 1 2 196 5 204 2.73e-64 197
MS.gene81774.t1 AT2G27370 55.978 184 74 1 12 195 43 219 6.75e-64 197
MS.gene81774.t1 AT5G06200 58.586 198 77 2 2 195 5 201 1.03e-63 196
MS.gene81774.t1 AT5G15290 44.000 150 84 0 27 176 17 166 4.56e-44 145
MS.gene81774.t1 AT1G14160 40.000 165 99 0 32 196 45 209 8.32e-42 140
MS.gene81774.t1 AT1G03700 30.769 169 99 3 21 189 1 151 1.95e-17 76.3
MS.gene81774.t1 AT1G17200 26.984 189 125 5 9 193 2 181 2.36e-14 68.9
MS.gene81774.t1 AT4G03540 32.258 124 75 2 55 178 26 140 1.06e-13 66.2
MS.gene81774.t1 AT3G06390 27.820 133 91 2 38 165 37 169 5.71e-11 59.7

Find 43 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCCATTTGCAACCAATT+TGG 0.187527 1.3:-43232665 MS.gene81774:CDS
GTTGGTTGTGATGTCTTCTT+TGG 0.281389 1.3:-43232576 MS.gene81774:CDS
GTGATGTCTTCTTTGGCTCT+TGG 0.299534 1.3:-43232569 MS.gene81774:CDS
TCTTAGAACAAAATCCATTA+TGG 0.318159 1.3:+43233732 None:intergenic
ATGATGGTTACTATGGAAAA+TGG 0.329026 1.3:+43233323 None:intergenic
TGATGTCTTCTTTGGCTCTT+GGG 0.348436 1.3:-43232568 MS.gene81774:CDS
AGCAGCTGCCATGGTTTACT+TGG 0.359773 1.3:-43232717 MS.gene81774:CDS
GGCATTTGTGGGTGGCTACC+TGG 0.401958 1.3:-43233357 MS.gene81774:CDS
AATGTCAACCACTATTGATA+TGG 0.415102 1.3:-43233839 MS.gene81774:CDS
AGGGCGAATGATGGTTACTA+TGG 0.422728 1.3:+43233316 None:intergenic
CATAACTAGCTTCAAATTGA+AGG 0.426220 1.3:+43233629 None:intergenic
ATTGGAGCCACTGCAAGGCC+TGG 0.429435 1.3:-43233775 MS.gene81774:CDS
ATGGCAATCAAGACTCAAAC+TGG 0.433393 1.3:-43232688 MS.gene81774:CDS
ATCTCCAAATTGGTTGCAAA+TGG 0.439108 1.3:+43232661 None:intergenic
TGTTATTGCAATGGCATTTG+TGG 0.446696 1.3:-43233369 MS.gene81774:CDS
GTTATTGCAATGGCATTTGT+GGG 0.458726 1.3:-43233368 MS.gene81774:CDS
TGATTCTGCCATATCAATAG+TGG 0.478795 1.3:+43233831 None:intergenic
ATCATTCGCCCTCATGCACC+TGG 0.484461 1.3:-43233305 MS.gene81774:CDS
TGGTTACTATGGAAAATGGT+AGG 0.486275 1.3:+43233327 None:intergenic
TGGTGCTTCAGCAGCTGCCA+TGG 0.489524 1.3:-43232726 MS.gene81774:CDS
TGAAGGAACTGAGTGAAGAA+AGG 0.493692 1.3:+43233646 None:intergenic
CCACTGCAAGGCCTGGAGGA+TGG 0.524990 1.3:-43233768 MS.gene81774:CDS
TGCAGCAAGAGAAGCTGCTA+TGG 0.527344 1.3:+43233702 None:intergenic
CCATCCTCCAGGCCTTGCAG+TGG 0.532661 1.3:+43233768 None:intergenic
ATGAGGAAAAGCCTTGGTCC+AGG 0.542585 1.3:+43233287 None:intergenic
CTTTAATTGGAGCCACTGCA+AGG 0.554612 1.3:-43233780 MS.gene81774:CDS
GGTTACTATGGAAAATGGTA+GGG 0.561081 1.3:+43233328 None:intergenic
AGCCTTGGTCCAGGTGCATG+AGG 0.573726 1.3:+43233296 None:intergenic
AGGTGCATGAGGGCGAATGA+TGG 0.573876 1.3:+43233307 None:intergenic
GCCCTCATGCACCTGGACCA+AGG 0.587519 1.3:-43233298 MS.gene81774:CDS
TGCTGAAGCACCACTAGCAG+TGG 0.592321 1.3:+43232736 None:intergenic
AATCTGATCACTAGTTGCCA+TGG 0.596028 1.3:+43233672 None:intergenic
ATTGCAATGGCATTTGTGGG+TGG 0.596308 1.3:-43233365 MS.gene81774:CDS
ATGACATTAGCCACTGCTAG+TGG 0.602800 1.3:-43232746 MS.gene81774:CDS
TTACCGTGTCTGCGATGATG+AGG 0.605858 1.3:+43233270 None:intergenic
ATGGTTTACTTGGCACACAA+TGG 0.608727 1.3:-43232707 MS.gene81774:CDS
GCGATGATGAGGAAAAGCCT+TGG 0.613791 1.3:+43233281 None:intergenic
TTTCCTCATCATCGCAGACA+CGG 0.615275 1.3:-43233273 MS.gene81774:intron
GCCTTGGTCCAGGTGCATGA+GGG 0.623258 1.3:+43233297 None:intergenic
GGAGCCACTGCAAGGCCTGG+AGG 0.665040 1.3:-43233772 MS.gene81774:CDS
TCTTGCTGCAACTATCACCA+TGG 0.694318 1.3:-43233689 MS.gene81774:CDS
AAAATGGTAGGGATAAGACC+AGG 0.708096 1.3:+43233339 None:intergenic
ATTGTGTGCCAAGTAAACCA+TGG 0.791669 1.3:+43232709 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAATCAATAAAGCAA+TGG + chr1.3:43233317-43233336 None:intergenic 15.0%
!! ACTCTAATTAAGAATTAATT+AGG + chr1.3:43233168-43233187 None:intergenic 15.0%
!! TAAGAATTAATTAGGAAATT+AGG + chr1.3:43233160-43233179 None:intergenic 15.0%
!! TCATGTAAAAAAAATTTGAA+GGG + chr1.3:43233211-43233230 None:intergenic 15.0%
!! TTCATGTAAAAAAAATTTGA+AGG + chr1.3:43233212-43233231 None:intergenic 15.0%
!!! AAATTATTTGTTTAAGTTAG+AGG + chr1.3:43232829-43232848 None:intergenic 15.0%
!!! AGAAACTATTATTTTAAAAG+TGG - chr1.3:43233541-43233560 MS.gene81774:intron 15.0%
!! AATGTTTATTGTAAATATGC+AGG - chr1.3:43233000-43233019 MS.gene81774:intron 20.0%
!! ATGAACATATATATTCTAGT+AGG - chr1.3:43233227-43233246 MS.gene81774:intron 20.0%
!!! GTTAAGTACTTTTAAAAAAC+TGG - chr1.3:43233512-43233531 MS.gene81774:intron 20.0%
! AATGAGGAAATGAACTATTA+AGG - chr1.3:43233427-43233446 MS.gene81774:intron 25.0%
! GGTCATTTGAAAATAAATAG+AGG + chr1.3:43233296-43233315 None:intergenic 25.0%
! TCTTAGAACAAAATCCATTA+TGG + chr1.3:43232668-43232687 None:intergenic 25.0%
!! AATGAAAAAGCTGCTTTAAT+TGG - chr1.3:43232604-43232623 MS.gene81774:CDS 25.0%
!!! ATGGATTTTGTTCTAAGATT+AGG - chr1.3:43232670-43232689 MS.gene81774:CDS 25.0%
ATGATGGTTACTATGGAAAA+TGG + chr1.3:43233077-43233096 None:intergenic 30.0%
ATTACTCCTTGTGTTTCTTT+CGG - chr1.3:43233270-43233289 MS.gene81774:intron 30.0%
GAAGATAAATTGCTACAATG+AGG - chr1.3:43233411-43233430 MS.gene81774:intron 30.0%
TAATGTCATAAACACCTACA+CGG + chr1.3:43233640-43233659 None:intergenic 30.0%
TGGACTAAATAAATACACCT+AGG + chr1.3:43232874-43232893 None:intergenic 30.0%
! AATACACCTAGGTTTTACAA+TGG + chr1.3:43232863-43232882 None:intergenic 30.0%
! CATAACTAGCTTCAAATTGA+AGG + chr1.3:43232771-43232790 None:intergenic 30.0%
! GTGTAGTTCAAATTTTGTGT+TGG + chr1.3:43232894-43232913 None:intergenic 30.0%
!!! CAGGTTTTTTGTTATTGCAA+TGG - chr1.3:43233019-43233038 MS.gene81774:intron 30.0%
!!! TATTGCTGTGGTTGTTTTAA+TGG - chr1.3:43233797-43233816 MS.gene81774:CDS 30.0%
ATCTCCAAATTGGTTGCAAA+TGG + chr1.3:43233739-43233758 None:intergenic 35.0%
GAGCACAAAAATCTCCAAAT+TGG + chr1.3:43233749-43233768 None:intergenic 35.0%
GAGGTTTAAATGTGACATAC+TGG + chr1.3:43232810-43232829 None:intergenic 35.0%
GGTTACTATGGAAAATGGTA+GGG + chr1.3:43233072-43233091 None:intergenic 35.0%
GTTATTGCAATGGCATTTGT+GGG - chr1.3:43233029-43233048 MS.gene81774:intron 35.0%
TGATTCTGCCATATCAATAG+TGG + chr1.3:43232569-43232588 None:intergenic 35.0%
TGGTTACTATGGAAAATGGT+AGG + chr1.3:43233073-43233092 None:intergenic 35.0%
TGTTATTGCAATGGCATTTG+TGG - chr1.3:43233028-43233047 MS.gene81774:intron 35.0%
! AAACTCAAACTTTTCCGTGT+AGG - chr1.3:43233623-43233642 MS.gene81774:CDS 35.0%
! GAAAAAAGGCATAGCCATAA+TGG - chr1.3:43232651-43232670 MS.gene81774:CDS 35.0%
! GATTTTTGTGCTCAAACAAG+TGG - chr1.3:43233756-43233775 MS.gene81774:CDS 35.0%
!! TGTGTCATGTTTTATTGCTG+TGG - chr1.3:43233785-43233804 MS.gene81774:CDS 35.0%
!!! TGTGGTTGTTTTAATGGTGT+TGG - chr1.3:43233803-43233822 MS.gene81774:CDS 35.0%
AAAATGGTAGGGATAAGACC+AGG + chr1.3:43233061-43233080 None:intergenic 40.0%
AATCTGATCACTAGTTGCCA+TGG + chr1.3:43232728-43232747 None:intergenic 40.0%
ATGGCAATCAAGACTCAAAC+TGG - chr1.3:43233709-43233728 MS.gene81774:CDS 40.0%
ATGGTTTACTTGGCACACAA+TGG - chr1.3:43233690-43233709 MS.gene81774:CDS 40.0%
ATTGTGTGCCAAGTAAACCA+TGG + chr1.3:43233691-43233710 None:intergenic 40.0%
GTTGGTTGTGATGTCTTCTT+TGG - chr1.3:43233821-43233840 MS.gene81774:CDS 40.0%
TGAAGGAACTGAGTGAAGAA+AGG + chr1.3:43232754-43232773 None:intergenic 40.0%
! ACACCTAGGTTTTACAATGG+AGG + chr1.3:43232860-43232879 None:intergenic 40.0%
! CACCTAGGTTTTACAATGGA+GGG + chr1.3:43232859-43232878 None:intergenic 40.0%
! CTTGCCATTTGCAACCAATT+TGG - chr1.3:43233732-43233751 MS.gene81774:CDS 40.0%
!! TGATGTCTTCTTTGGCTCTT+GGG - chr1.3:43233829-43233848 MS.gene81774:CDS 40.0%
AGGGCGAATGATGGTTACTA+TGG + chr1.3:43233084-43233103 None:intergenic 45.0%
ATGACATTAGCCACTGCTAG+TGG - chr1.3:43233651-43233670 MS.gene81774:CDS 45.0%
ATTGCAATGGCATTTGTGGG+TGG - chr1.3:43233032-43233051 MS.gene81774:intron 45.0%
CTCCCTCCATTGTAAAACCT+AGG - chr1.3:43232854-43232873 MS.gene81774:intron 45.0%
CTTTAATTGGAGCCACTGCA+AGG - chr1.3:43232617-43232636 MS.gene81774:CDS 45.0%
TAGAGGCCGAAAGAAACACA+AGG + chr1.3:43233279-43233298 None:intergenic 45.0%
TCTTGCTGCAACTATCACCA+TGG - chr1.3:43232708-43232727 MS.gene81774:CDS 45.0%
TTTCCTCATCATCGCAGACA+CGG - chr1.3:43233124-43233143 MS.gene81774:intron 45.0%
!! GTGATGTCTTCTTTGGCTCT+TGG - chr1.3:43233828-43233847 MS.gene81774:CDS 45.0%
!!! ATGCCTTTTTTCCATCCTCC+AGG + chr1.3:43232643-43232662 None:intergenic 45.0%
AGCAGCTGCCATGGTTTACT+TGG - chr1.3:43233680-43233699 MS.gene81774:CDS 50.0%
AGGCCTGGAGGATGGAAAAA+AGG - chr1.3:43232637-43232656 MS.gene81774:CDS 50.0%
ATGAGGAAAAGCCTTGGTCC+AGG + chr1.3:43233113-43233132 None:intergenic 50.0%
GCGATGATGAGGAAAAGCCT+TGG + chr1.3:43233119-43233138 None:intergenic 50.0%
TGCAGCAAGAGAAGCTGCTA+TGG + chr1.3:43232698-43232717 None:intergenic 50.0%
TTACCGTGTCTGCGATGATG+AGG + chr1.3:43233130-43233149 None:intergenic 50.0%
AGGTGCATGAGGGCGAATGA+TGG + chr1.3:43233093-43233112 None:intergenic 55.0%
ATCATTCGCCCTCATGCACC+TGG - chr1.3:43233092-43233111 MS.gene81774:intron 55.0%
! TGCTGAAGCACCACTAGCAG+TGG + chr1.3:43233664-43233683 None:intergenic 55.0%
AGCCTTGGTCCAGGTGCATG+AGG + chr1.3:43233104-43233123 None:intergenic 60.0%
ATTGGAGCCACTGCAAGGCC+TGG - chr1.3:43232622-43232641 MS.gene81774:CDS 60.0%
GCCTTGGTCCAGGTGCATGA+GGG + chr1.3:43233103-43233122 None:intergenic 60.0%
GGCATTTGTGGGTGGCTACC+TGG - chr1.3:43233040-43233059 MS.gene81774:intron 60.0%
TGGTGCTTCAGCAGCTGCCA+TGG - chr1.3:43233671-43233690 MS.gene81774:CDS 60.0%
CCACTGCAAGGCCTGGAGGA+TGG - chr1.3:43232629-43232648 MS.gene81774:CDS 65.0%
CCATCCTCCAGGCCTTGCAG+TGG + chr1.3:43232632-43232651 None:intergenic 65.0%
GCCCTCATGCACCTGGACCA+AGG - chr1.3:43233099-43233118 MS.gene81774:intron 65.0%
GGAGCCACTGCAAGGCCTGG+AGG - chr1.3:43232625-43232644 MS.gene81774:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 43232559 43233860 43232559 ID=MS.gene81774
chr1.3 mRNA 43232559 43233860 43232559 ID=MS.gene81774.t1;Parent=MS.gene81774
chr1.3 exon 43233610 43233860 43233610 ID=MS.gene81774.t1.exon1;Parent=MS.gene81774.t1
chr1.3 CDS 43233610 43233860 43233610 ID=cds.MS.gene81774.t1;Parent=MS.gene81774.t1
chr1.3 exon 43233274 43233397 43233274 ID=MS.gene81774.t1.exon2;Parent=MS.gene81774.t1
chr1.3 CDS 43233274 43233397 43233274 ID=cds.MS.gene81774.t1;Parent=MS.gene81774.t1
chr1.3 exon 43232559 43232774 43232559 ID=MS.gene81774.t1.exon3;Parent=MS.gene81774.t1
chr1.3 CDS 43232559 43232774 43232559 ID=cds.MS.gene81774.t1;Parent=MS.gene81774.t1
Gene Sequence

>MS.gene81774

ATGTCAACCACTATTGATATGGCAGAATCAAGCAACGCTCATGTTAATGAAAAAGCTGCTTTAATTGGAGCCACTGCAAGGCCTGGAGGATGGAAAAAAGGCATAGCCATAATGGATTTTGTTCTAAGATTAGGTGCCATAGCAGCTTCTCTTGCTGCAACTATCACCATGGCAACTAGTGATCAGATTCTTCCTTTCTTCACTCAGTTCCTTCAATTTGAAGCTAGTTATGATAGCTTTTCAACTTTCCAGTATGTCACATTTAAACCTCTAACTTAAACAAATAATTTATATACTCCCTCCATTGTAAAACCTAGGTGTATTTATTTAGTCCAACACAAAATTTGAACTACACACATATTTATATACTTTTTATTTATATTTTGTTATTAATTTTGTGCTATATTCATAGAAATTAAATTAATTATGTCAAAAAATATTAATGTTTATTGTAAATATGCAGGTTTTTTGTTATTGCAATGGCATTTGTGGGTGGCTACCTGGTCTTATCCCTACCATTTTCCATAGTAACCATCATTCGCCCTCATGCACCTGGACCAAGGCTTTTCCTCATCATCGCAGACACGGTAATTATGCTCCTAATTTCCTAATTAATTCTTAATTAGAGTAAAATAATTTTACTTGAGAACCCTTCAAATTTTTTTTACATGAACATATATATTCTAGTAGGAACACATTTTAAATATAATTATTACTCCTTGTGTTTCTTTCGGCCTCTATTTATTTTCAAATGACCATTGCTTTATTGATTTTTTTTTAATATTCACATGTTATTTTCAAGATTAAAATTACATTTTTTTGCAATGTATAGTCATAATTAATTTATTTCAAGAAGATAAATTGCTACAATGAGGAAATGAACTATTAAGGATATAACAATAAAAATACTAACAATTTTATATAAAAAATAAAATAATACATTGATTTATTTAGTTAAGTACTTTTAAAAAACTGGAAAAAGAGAAACTATTATTTTAAAAGTGGTACTAGTAAATGTTTTATAATTATTTTTGTAAAGATTTGAAATTTTAGATTAAAGTTACAAACTCAAACTTTTCCGTGTAGGTGTTTATGACATTAGCCACTGCTAGTGGTGCTTCAGCAGCTGCCATGGTTTACTTGGCACACAATGGCAATCAAGACTCAAACTGGCTTGCCATTTGCAACCAATTTGGAGATTTTTGTGCTCAAACAAGTGGAGCAGTTGTGTCATGTTTTATTGCTGTGGTTGTTTTAATGGTGTTGGTTGTGATGTCTTCTTTGGCTCTTGGGAAGCATTGA

Protein sequence

>MS.gene81774.t1

MSTTIDMAESSNAHVNEKAALIGATARPGGWKKGIAIMDFVLRLGAIAASLAATITMATSDQILPFFTQFLQFEASYDSFSTFQFFVIAMAFVGGYLVLSLPFSIVTIIRPHAPGPRLFLIIADTVFMTLATASGASAAAMVYLAHNGNQDSNWLAICNQFGDFCAQTSGAVVSCFIAVVVLMVLVVMSSLALGKH