Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066937.t1 | XP_003628224.1 | 95.9 | 218 | 9 | 0 | 1 | 218 | 19 | 236 | 2.00E-119 | 438.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066937.t1 | Q43675 | 54.0 | 198 | 78 | 4 | 13 | 204 | 68 | 258 | 1.8e-52 | 207.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066937.t1 | G7L8H6 | 95.9 | 218 | 9 | 0 | 1 | 218 | 19 | 236 | 1.5e-119 | 438.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04926 | MS.gene066937 | 0.808158 | 3.55E-50 | -1.69E-46 |
| MS.gene049549 | MS.gene066937 | 0.848037 | 7.91E-60 | -1.69E-46 |
| MS.gene049993 | MS.gene066937 | -0.801029 | 1.09E-48 | -1.69E-46 |
| MS.gene050096 | MS.gene066937 | 0.811287 | 7.55E-51 | -1.69E-46 |
| MS.gene050133 | MS.gene066937 | 0.802703 | 4.95E-49 | -1.69E-46 |
| MS.gene050329 | MS.gene066937 | 0.813785 | 2.15E-51 | -1.69E-46 |
| MS.gene050397 | MS.gene066937 | -0.816132 | 6.47E-52 | -1.69E-46 |
| MS.gene050426 | MS.gene066937 | 0.822381 | 2.44E-53 | -1.69E-46 |
| MS.gene050464 | MS.gene066937 | -0.816775 | 4.65E-52 | -1.69E-46 |
| MS.gene050529 | MS.gene066937 | -0.81333 | 2.70E-51 | -1.69E-46 |
| MS.gene050670 | MS.gene066937 | -0.821942 | 3.09E-53 | -1.69E-46 |
| MS.gene050720 | MS.gene066937 | 0.804385 | 2.22E-49 | -1.69E-46 |
| MS.gene050809 | MS.gene066937 | 0.805033 | 1.62E-49 | -1.69E-46 |
| MS.gene05104 | MS.gene066937 | 0.813508 | 2.47E-51 | -1.69E-46 |
| MS.gene051131 | MS.gene066937 | 0.807054 | 6.10E-50 | -1.69E-46 |
| MS.gene051158 | MS.gene066937 | 0.868534 | 6.08E-66 | -1.69E-46 |
| MS.gene051385 | MS.gene066937 | 0.814473 | 1.51E-51 | -1.69E-46 |
| MS.gene051525 | MS.gene066937 | -0.81925 | 1.28E-52 | -1.69E-46 |
| MS.gene051552 | MS.gene066937 | 0.817984 | 2.48E-52 | -1.69E-46 |
| MS.gene051996 | MS.gene066937 | 0.873478 | 1.43E-67 | -1.69E-46 |
| MS.gene052011 | MS.gene066937 | 0.827838 | 1.25E-54 | -1.69E-46 |
| MS.gene052128 | MS.gene066937 | 0.809173 | 2.16E-50 | -1.69E-46 |
| MS.gene052134 | MS.gene066937 | -0.813352 | 2.67E-51 | -1.69E-46 |
| MS.gene052135 | MS.gene066937 | -0.808021 | 3.80E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene066937 | 0.823982 | 1.03E-53 | -1.69E-46 |
| MS.gene052223 | MS.gene066937 | 0.873165 | 1.82E-67 | -1.69E-46 |
| MS.gene052253 | MS.gene066937 | -0.801779 | 7.67E-49 | -1.69E-46 |
| MS.gene05233 | MS.gene066937 | 0.83481 | 2.42E-56 | -1.69E-46 |
| MS.gene05240 | MS.gene066937 | 0.843071 | 1.76E-58 | -1.69E-46 |
| MS.gene05244 | MS.gene066937 | 0.810783 | 9.71E-51 | -1.69E-46 |
| MS.gene052705 | MS.gene066937 | 0.801072 | 1.07E-48 | -1.69E-46 |
| MS.gene052706 | MS.gene066937 | 0.832143 | 1.12E-55 | -1.69E-46 |
| MS.gene052805 | MS.gene066937 | 0.809343 | 1.98E-50 | -1.69E-46 |
| MS.gene053406 | MS.gene066937 | 0.85144 | 8.87E-61 | -1.69E-46 |
| MS.gene054138 | MS.gene066937 | 0.817193 | 3.74E-52 | -1.69E-46 |
| MS.gene054146 | MS.gene066937 | 0.818857 | 1.57E-52 | -1.69E-46 |
| MS.gene054147 | MS.gene066937 | 0.829951 | 3.86E-55 | -1.69E-46 |
| MS.gene054183 | MS.gene066937 | -0.815078 | 1.11E-51 | -1.69E-46 |
| MS.gene054277 | MS.gene066937 | 0.865924 | 4.15E-65 | -1.69E-46 |
| MS.gene054290 | MS.gene066937 | 0.811602 | 6.45E-51 | -1.69E-46 |
| MS.gene054421 | MS.gene066937 | 0.809225 | 2.10E-50 | -1.69E-46 |
| MS.gene054742 | MS.gene066937 | 0.890722 | 7.62E-74 | -1.69E-46 |
| MS.gene055240 | MS.gene066937 | 0.8297 | 4.45E-55 | -1.69E-46 |
| MS.gene055277 | MS.gene066937 | -0.805325 | 1.41E-49 | -1.69E-46 |
| MS.gene055289 | MS.gene066937 | -0.803451 | 3.46E-49 | -1.69E-46 |
| MS.gene055369 | MS.gene066937 | -0.804712 | 1.89E-49 | -1.69E-46 |
| MS.gene055370 | MS.gene066937 | -0.803665 | 3.13E-49 | -1.69E-46 |
| MS.gene055533 | MS.gene066937 | -0.81432 | 1.64E-51 | -1.69E-46 |
| MS.gene055601 | MS.gene066937 | 0.844708 | 6.40E-59 | -1.69E-46 |
| MS.gene056076 | MS.gene066937 | 0.83208 | 1.16E-55 | -1.69E-46 |
| MS.gene056330 | MS.gene066937 | 0.883427 | 4.53E-71 | -1.69E-46 |
| MS.gene056514 | MS.gene066937 | -0.807319 | 5.36E-50 | -1.69E-46 |
| MS.gene056590 | MS.gene066937 | 0.816082 | 6.64E-52 | -1.69E-46 |
| MS.gene056591 | MS.gene066937 | 0.814034 | 1.89E-51 | -1.69E-46 |
| MS.gene056680 | MS.gene066937 | 0.819436 | 1.16E-52 | -1.69E-46 |
| MS.gene056688 | MS.gene066937 | 0.801238 | 9.89E-49 | -1.69E-46 |
| MS.gene056690 | MS.gene066937 | 0.836608 | 8.47E-57 | -1.69E-46 |
| MS.gene056691 | MS.gene066937 | 0.818391 | 2.01E-52 | -1.69E-46 |
| MS.gene056748 | MS.gene066937 | 0.801166 | 1.02E-48 | -1.69E-46 |
| MS.gene056899 | MS.gene066937 | 0.847534 | 1.09E-59 | -1.69E-46 |
| MS.gene056920 | MS.gene066937 | 0.869286 | 3.47E-66 | -1.69E-46 |
| MS.gene056922 | MS.gene066937 | 0.856165 | 3.87E-62 | -1.69E-46 |
| MS.gene056923 | MS.gene066937 | 0.859096 | 5.24E-63 | -1.69E-46 |
| MS.gene056924 | MS.gene066937 | 0.861011 | 1.38E-63 | -1.69E-46 |
| MS.gene056925 | MS.gene066937 | 0.810839 | 9.44E-51 | -1.69E-46 |
| MS.gene057261 | MS.gene066937 | 0.819432 | 1.17E-52 | -1.69E-46 |
| MS.gene057544 | MS.gene066937 | 0.841271 | 5.26E-58 | -1.69E-46 |
| MS.gene057749 | MS.gene066937 | -0.811559 | 6.59E-51 | -1.69E-46 |
| MS.gene057823 | MS.gene066937 | -0.805298 | 1.43E-49 | -1.69E-46 |
| MS.gene058177 | MS.gene066937 | 0.814877 | 1.23E-51 | -1.69E-46 |
| MS.gene058218 | MS.gene066937 | 0.83989 | 1.21E-57 | -1.69E-46 |
| MS.gene058418 | MS.gene066937 | 0.812433 | 4.25E-51 | -1.69E-46 |
| MS.gene058928 | MS.gene066937 | -0.809744 | 1.63E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene066937 | 0.835182 | 1.95E-56 | -1.69E-46 |
| MS.gene05907 | MS.gene066937 | -0.808129 | 3.60E-50 | -1.69E-46 |
| MS.gene059294 | MS.gene066937 | -0.813303 | 2.74E-51 | -1.69E-46 |
| MS.gene059328 | MS.gene066937 | 0.813662 | 2.28E-51 | -1.69E-46 |
| MS.gene059402 | MS.gene066937 | 0.807653 | 4.55E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene066937 | -0.830601 | 2.68E-55 | -1.69E-46 |
| MS.gene059891 | MS.gene066937 | 0.806924 | 6.49E-50 | -1.69E-46 |
| MS.gene059892 | MS.gene066937 | 0.837723 | 4.39E-57 | -1.69E-46 |
| MS.gene059955 | MS.gene066937 | 0.819143 | 1.36E-52 | -1.69E-46 |
| MS.gene060087 | MS.gene066937 | 0.866372 | 2.99E-65 | -1.69E-46 |
| MS.gene060205 | MS.gene066937 | 0.80222 | 6.22E-49 | -1.69E-46 |
| MS.gene060403 | MS.gene066937 | -0.82042 | 6.93E-53 | -1.69E-46 |
| MS.gene060578 | MS.gene066937 | 0.809154 | 2.18E-50 | -1.69E-46 |
| MS.gene061092 | MS.gene066937 | 0.800265 | 1.56E-48 | -1.69E-46 |
| MS.gene061094 | MS.gene066937 | 0.807716 | 4.41E-50 | -1.69E-46 |
| MS.gene061154 | MS.gene066937 | -0.80424 | 2.38E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066937.t1 | MTR_8g046000 | 95.872 | 218 | 9 | 0 | 1 | 218 | 19 | 236 | 6.95e-161 | 444 |
| MS.gene066937.t1 | MTR_8g045890 | 62.921 | 178 | 62 | 1 | 45 | 218 | 268 | 445 | 1.97e-76 | 237 |
| MS.gene066937.t1 | MTR_8g045890 | 62.921 | 178 | 62 | 1 | 45 | 218 | 309 | 486 | 8.50e-76 | 236 |
| MS.gene066937.t1 | MTR_8g045890 | 62.921 | 178 | 62 | 1 | 45 | 218 | 346 | 523 | 1.69e-75 | 236 |
| MS.gene066937.t1 | MTR_8g445170 | 62.500 | 176 | 61 | 2 | 43 | 218 | 227 | 397 | 8.28e-75 | 231 |
| MS.gene066937.t1 | MTR_8g445140 | 50.820 | 244 | 68 | 5 | 13 | 204 | 59 | 302 | 1.06e-74 | 228 |
| MS.gene066937.t1 | MTR_8g045880 | 61.458 | 192 | 66 | 2 | 35 | 218 | 137 | 328 | 6.15e-74 | 227 |
| MS.gene066937.t1 | MTR_8g045880 | 65.143 | 175 | 59 | 1 | 46 | 218 | 189 | 363 | 1.87e-73 | 227 |
| MS.gene066937.t1 | MTR_8g045880 | 65.143 | 175 | 59 | 1 | 46 | 218 | 191 | 365 | 2.89e-73 | 226 |
| MS.gene066937.t1 | MTR_1g045860 | 56.039 | 207 | 84 | 3 | 13 | 218 | 69 | 269 | 3.11e-73 | 223 |
| MS.gene066937.t1 | MTR_2g081770 | 49.490 | 196 | 91 | 4 | 13 | 204 | 79 | 270 | 6.53e-61 | 192 |
| MS.gene066937.t1 | MTR_2g081610 | 45.408 | 196 | 98 | 3 | 11 | 204 | 71 | 259 | 9.67e-60 | 189 |
| MS.gene066937.t1 | MTR_2g081590 | 46.392 | 194 | 95 | 3 | 13 | 204 | 73 | 259 | 2.41e-57 | 182 |
| MS.gene066937.t1 | MTR_8g044290 | 61.224 | 147 | 57 | 0 | 35 | 181 | 197 | 343 | 6.00e-56 | 184 |
| MS.gene066937.t1 | MTR_1g008460 | 35.681 | 213 | 111 | 7 | 12 | 203 | 88 | 295 | 4.47e-35 | 126 |
| MS.gene066937.t1 | MTR_3g109490 | 35.841 | 226 | 112 | 9 | 7 | 204 | 101 | 321 | 5.45e-35 | 126 |
| MS.gene066937.t1 | MTR_1g008470 | 38.532 | 218 | 101 | 9 | 12 | 203 | 88 | 298 | 8.99e-34 | 122 |
| MS.gene066937.t1 | MTR_1g008590 | 38.073 | 218 | 103 | 9 | 12 | 203 | 112 | 323 | 5.22e-33 | 121 |
| MS.gene066937.t1 | MTR_0312s0030 | 38.073 | 218 | 103 | 9 | 12 | 203 | 112 | 323 | 7.88e-33 | 120 |
| MS.gene066937.t1 | MTR_1g007520 | 38.073 | 218 | 103 | 9 | 12 | 203 | 112 | 323 | 7.88e-33 | 120 |
| MS.gene066937.t1 | MTR_1g008560 | 37.500 | 216 | 110 | 8 | 12 | 203 | 114 | 328 | 7.22e-32 | 118 |
| MS.gene066937.t1 | MTR_1g008580 | 43.066 | 137 | 71 | 4 | 74 | 203 | 34 | 170 | 1.13e-31 | 114 |
| MS.gene066937.t1 | MTR_1g008530 | 42.336 | 137 | 72 | 4 | 74 | 203 | 86 | 222 | 1.66e-31 | 115 |
| MS.gene066937.t1 | MTR_1g008510 | 37.963 | 216 | 105 | 9 | 12 | 203 | 114 | 324 | 1.73e-31 | 117 |
| MS.gene066937.t1 | MTR_1g008500 | 35.000 | 220 | 109 | 8 | 12 | 203 | 170 | 383 | 1.90e-31 | 118 |
| MS.gene066937.t1 | MTR_1g008450 | 32.127 | 221 | 111 | 9 | 12 | 203 | 101 | 311 | 1.76e-26 | 103 |
| MS.gene066937.t1 | MTR_3g107830 | 39.865 | 148 | 79 | 5 | 67 | 204 | 108 | 255 | 1.44e-22 | 92.8 |
| MS.gene066937.t1 | MTR_3g116270 | 30.734 | 218 | 125 | 7 | 13 | 206 | 292 | 507 | 4.30e-22 | 94.0 |
| MS.gene066937.t1 | MTR_3g116320 | 31.651 | 218 | 123 | 7 | 13 | 206 | 474 | 689 | 5.23e-22 | 94.0 |
| MS.gene066937.t1 | MTR_4g069680 | 38.514 | 148 | 81 | 6 | 67 | 204 | 139 | 286 | 2.33e-20 | 87.4 |
| MS.gene066937.t1 | MTR_3g116410 | 38.514 | 148 | 81 | 5 | 67 | 204 | 128 | 275 | 8.74e-20 | 85.5 |
| MS.gene066937.t1 | MTR_4g069570 | 37.013 | 154 | 83 | 6 | 65 | 205 | 184 | 336 | 1.46e-18 | 82.8 |
| MS.gene066937.t1 | MTR_3g116380 | 35.065 | 154 | 90 | 6 | 61 | 205 | 151 | 303 | 3.56e-18 | 81.6 |
| MS.gene066937.t1 | MTR_4g069550 | 31.126 | 151 | 92 | 6 | 67 | 205 | 313 | 463 | 1.21e-12 | 66.6 |
| MS.gene066937.t1 | MTR_5g033350 | 30.968 | 155 | 96 | 5 | 57 | 202 | 46 | 198 | 2.38e-12 | 63.9 |
| MS.gene066937.t1 | MTR_4g133840 | 32.298 | 161 | 97 | 7 | 52 | 202 | 28 | 186 | 6.36e-12 | 62.4 |
| MS.gene066937.t1 | MTR_5g033340 | 30.968 | 155 | 96 | 5 | 57 | 202 | 225 | 377 | 9.13e-12 | 63.9 |
| MS.gene066937.t1 | MTR_4g068420 | 31.343 | 134 | 73 | 6 | 78 | 205 | 1 | 121 | 3.20e-11 | 59.3 |
| MS.gene066937.t1 | MTR_5g033330 | 30.323 | 155 | 97 | 5 | 57 | 202 | 464 | 616 | 5.44e-11 | 62.0 |
| MS.gene066937.t1 | MTR_6g086530 | 31.469 | 143 | 86 | 6 | 74 | 204 | 63 | 205 | 7.53e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066937.t1 | AT5G25610 | 35.294 | 221 | 119 | 7 | 7 | 203 | 170 | 390 | 9.73e-32 | 119 |
| MS.gene066937.t1 | AT1G70370 | 32.192 | 146 | 87 | 5 | 67 | 202 | 479 | 622 | 4.61e-13 | 68.2 |
| MS.gene066937.t1 | AT1G70370 | 32.192 | 146 | 87 | 5 | 67 | 202 | 479 | 622 | 4.61e-13 | 68.2 |
| MS.gene066937.t1 | AT1G49320 | 27.232 | 224 | 134 | 9 | 3 | 203 | 61 | 278 | 5.47e-12 | 63.9 |
Find 45 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAACTTCGTCAAGCAAATT+TGG | 0.185829 | 8.4:-55284368 | None:intergenic |
| TGTTCGAAATGGTTGAATAT+TGG | 0.231156 | 8.4:-55283940 | None:intergenic |
| GTTGAATATTGGAAAGCTTT+TGG | 0.243886 | 8.4:-55283929 | None:intergenic |
| ACGAAAATTAAGTCATATTC+AGG | 0.260765 | 8.4:+55284131 | MS.gene066937:CDS |
| AACTTGTCTTCTCCGATTGC+TGG | 0.301949 | 8.4:-55284056 | None:intergenic |
| ATTCAACCATTTCGAACAAT+AGG | 0.324074 | 8.4:+55283945 | MS.gene066937:CDS |
| GGCAAATTCCTTCATTGATT+CGG | 0.336511 | 8.4:-55284093 | None:intergenic |
| AACTTACATGGTTCCATTGT+TGG | 0.360469 | 8.4:+55284283 | MS.gene066937:CDS |
| GATTCACTTGCAACAGGAAT+AGG | 0.379182 | 8.4:-55284455 | None:intergenic |
| AACCATCCTATTGTTCGAAA+TGG | 0.382560 | 8.4:-55283951 | None:intergenic |
| TGGCAGACAGGGATGGTTCC+AGG | 0.385703 | 8.4:-55284401 | None:intergenic |
| CAGCCTTGTTACCAACAAAA+TGG | 0.391110 | 8.4:-55284421 | None:intergenic |
| TTTAATAATGCAGACATTGA+AGG | 0.399608 | 8.4:+55283852 | MS.gene066937:CDS |
| ATATCCAAATATCATCTAGA+TGG | 0.433321 | 8.4:-55284495 | None:intergenic |
| TTACTTCTTCCACTACATAT+TGG | 0.433811 | 8.4:-55284175 | None:intergenic |
| GTTCCATTGTTGGCCTCTGA+TGG | 0.457551 | 8.4:+55284293 | MS.gene066937:CDS |
| TCATCAGATTCACTTGCAAC+AGG | 0.481559 | 8.4:-55284461 | None:intergenic |
| TTGTCTTCTCCGATTGCTGG+TGG | 0.504772 | 8.4:-55284053 | None:intergenic |
| TTGGAAAATGACCTATACCC+TGG | 0.518567 | 8.4:+55283888 | MS.gene066937:CDS |
| AACATCCTCCGAATCAATGA+AGG | 0.526354 | 8.4:+55284085 | MS.gene066937:CDS |
| GATTCATACCTCTTGTGTCA+TGG | 0.531056 | 8.4:-55284346 | None:intergenic |
| GAATTTGCCATTTCAAAACT+CGG | 0.533199 | 8.4:+55284107 | MS.gene066937:CDS |
| TGTGGAGGTCCACCAGCAAT+CGG | 0.533362 | 8.4:+55284044 | MS.gene066937:CDS |
| AATTTGCCATTTCAAAACTC+GGG | 0.540236 | 8.4:+55284108 | MS.gene066937:CDS |
| TTGGTAACAAGGCTGTTGCT+TGG | 0.540533 | 8.4:+55284429 | MS.gene066937:CDS |
| GAAGTTCTCAAAGTCAAGCC+TGG | 0.543141 | 8.4:+55284383 | MS.gene066937:CDS |
| AACCATTTCGAACAATAGGA+TGG | 0.564743 | 8.4:+55283949 | MS.gene066937:CDS |
| TTGTTGATGCATTCACTTGG+TGG | 0.566717 | 8.4:-55284262 | None:intergenic |
| GAATGCATCAACAACTTACA+TGG | 0.567371 | 8.4:+55284271 | MS.gene066937:CDS |
| AAATTCCTTCATTGATTCGG+AGG | 0.568883 | 8.4:-55284090 | None:intergenic |
| TTACCAACAAAATGGCAGAC+AGG | 0.571358 | 8.4:-55284413 | None:intergenic |
| GTAAAGAAAATTGCAGACAA+AGG | 0.575220 | 8.4:+55284194 | MS.gene066937:CDS |
| TGGAACTAAAGTTAATGCAT+TGG | 0.576995 | 8.4:+55284313 | MS.gene066937:CDS |
| TCAACAATCTGGAAATGTAG+TGG | 0.583942 | 8.4:+55283992 | MS.gene066937:CDS |
| ATTAACTTTAGTTCCATCAG+AGG | 0.588740 | 8.4:-55284306 | None:intergenic |
| GTTCCATCAGAGGCCAACAA+TGG | 0.589021 | 8.4:-55284296 | None:intergenic |
| AAATCAAGACCAATATGTAG+TGG | 0.595851 | 8.4:+55284166 | MS.gene066937:CDS |
| TACCAACAAAATGGCAGACA+GGG | 0.596242 | 8.4:-55284412 | None:intergenic |
| AACAAAATGGCAGACAGGGA+TGG | 0.605104 | 8.4:-55284408 | None:intergenic |
| TATAGTCTTGACGAGATATG+TGG | 0.615207 | 8.4:+55284026 | MS.gene066937:CDS |
| TACCCTGGCAAGAAAATGAG+CGG | 0.639113 | 8.4:+55283903 | MS.gene066937:CDS |
| GCTTGTCACCATGACACAAG+AGG | 0.650773 | 8.4:+55284338 | MS.gene066937:CDS |
| AAGTTGTTGATGCATTCACT+TGG | 0.662304 | 8.4:-55284265 | None:intergenic |
| AATATCATCTAGATGGCACA+AGG | 0.675245 | 8.4:-55284488 | None:intergenic |
| AGTCTTGACGAGATATGTGG+AGG | 0.683695 | 8.4:+55284029 | MS.gene066937:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACGAAAATTAAGTCATATTC+AGG | + | chr8.4:55284131-55284150 | MS.gene066937:CDS | 25.0% |
| ! | TTTAATAATGCAGACATTGA+AGG | + | chr8.4:55283852-55283871 | MS.gene066937:CDS | 25.0% |
| AAATCAAGACCAATATGTAG+TGG | + | chr8.4:55284166-55284185 | MS.gene066937:CDS | 30.0% | |
| ATTCAACCATTTCGAACAAT+AGG | + | chr8.4:55283945-55283964 | MS.gene066937:CDS | 30.0% | |
| GTAAAGAAAATTGCAGACAA+AGG | + | chr8.4:55284194-55284213 | MS.gene066937:CDS | 30.0% | |
| TGGAACTAAAGTTAATGCAT+TGG | + | chr8.4:55284313-55284332 | MS.gene066937:CDS | 30.0% | |
| TGTTCGAAATGGTTGAATAT+TGG | - | chr8.4:55283943-55283962 | None:intergenic | 30.0% | |
| TTACTTCTTCCACTACATAT+TGG | - | chr8.4:55284178-55284197 | None:intergenic | 30.0% | |
| ! | AATTTGCCATTTCAAAACTC+GGG | + | chr8.4:55284108-55284127 | MS.gene066937:CDS | 30.0% |
| ! | ATTAACTTTAGTTCCATCAG+AGG | - | chr8.4:55284309-55284328 | None:intergenic | 30.0% |
| ! | GAATTTGCCATTTCAAAACT+CGG | + | chr8.4:55284107-55284126 | MS.gene066937:CDS | 30.0% |
| ! | GTTGAATATTGGAAAGCTTT+TGG | - | chr8.4:55283932-55283951 | None:intergenic | 30.0% |
| AACCATCCTATTGTTCGAAA+TGG | - | chr8.4:55283954-55283973 | None:intergenic | 35.0% | |
| AACCATTTCGAACAATAGGA+TGG | + | chr8.4:55283949-55283968 | MS.gene066937:CDS | 35.0% | |
| AACTTACATGGTTCCATTGT+TGG | + | chr8.4:55284283-55284302 | MS.gene066937:CDS | 35.0% | |
| AAGTTGTTGATGCATTCACT+TGG | - | chr8.4:55284268-55284287 | None:intergenic | 35.0% | |
| AGAACTTCGTCAAGCAAATT+TGG | - | chr8.4:55284371-55284390 | None:intergenic | 35.0% | |
| GAAAAAGCAACTCAACAATC+TGG | + | chr8.4:55283981-55284000 | MS.gene066937:CDS | 35.0% | |
| GAATGCATCAACAACTTACA+TGG | + | chr8.4:55284271-55284290 | MS.gene066937:CDS | 35.0% | |
| TCAACAATCTGGAAATGTAG+TGG | + | chr8.4:55283992-55284011 | MS.gene066937:CDS | 35.0% | |
| ! | GAATATTGGAAAGCTTTTGG+AGG | - | chr8.4:55283929-55283948 | None:intergenic | 35.0% |
| ! | GGCAAATTCCTTCATTGATT+CGG | - | chr8.4:55284096-55284115 | None:intergenic | 35.0% |
| ! | TATAGTCTTGACGAGATATG+TGG | + | chr8.4:55284026-55284045 | MS.gene066937:CDS | 35.0% |
| ! | TGAAGGCAAACTACTCTTTT+TGG | + | chr8.4:55283869-55283888 | MS.gene066937:CDS | 35.0% |
| !! | AAATTCCTTCATTGATTCGG+AGG | - | chr8.4:55284093-55284112 | None:intergenic | 35.0% |
| !!! | TGTTGAGTTGCTTTTTCGAT+CGG | - | chr8.4:55283978-55283997 | None:intergenic | 35.0% |
| AACATCCTCCGAATCAATGA+AGG | + | chr8.4:55284085-55284104 | MS.gene066937:CDS | 40.0% | |
| GATTCACTTGCAACAGGAAT+AGG | - | chr8.4:55284458-55284477 | None:intergenic | 40.0% | |
| GATTCATACCTCTTGTGTCA+TGG | - | chr8.4:55284349-55284368 | None:intergenic | 40.0% | |
| TACCAACAAAATGGCAGACA+GGG | - | chr8.4:55284415-55284434 | None:intergenic | 40.0% | |
| TCATCAGATTCACTTGCAAC+AGG | - | chr8.4:55284464-55284483 | None:intergenic | 40.0% | |
| TTACCAACAAAATGGCAGAC+AGG | - | chr8.4:55284416-55284435 | None:intergenic | 40.0% | |
| TTGGAAAATGACCTATACCC+TGG | + | chr8.4:55283888-55283907 | MS.gene066937:CDS | 40.0% | |
| TTGTTGATGCATTCACTTGG+TGG | - | chr8.4:55284265-55284284 | None:intergenic | 40.0% | |
| ! | TCATTTTCTTGCCAGGGTAT+AGG | - | chr8.4:55283902-55283921 | None:intergenic | 40.0% |
| !! | CAGCCTTGTTACCAACAAAA+TGG | - | chr8.4:55284424-55284443 | None:intergenic | 40.0% |
| !!! | CTGCCATTTTGTTGGTAACA+AGG | + | chr8.4:55284418-55284437 | MS.gene066937:CDS | 40.0% |
| !!! | TTTCGTCCCGAGTTTTGAAA+TGG | - | chr8.4:55284117-55284136 | None:intergenic | 40.0% |
| AACAAAATGGCAGACAGGGA+TGG | - | chr8.4:55284411-55284430 | None:intergenic | 45.0% | |
| AACTTGTCTTCTCCGATTGC+TGG | - | chr8.4:55284059-55284078 | None:intergenic | 45.0% | |
| AGTCTTGACGAGATATGTGG+AGG | + | chr8.4:55284029-55284048 | MS.gene066937:CDS | 45.0% | |
| GAAGTTCTCAAAGTCAAGCC+TGG | + | chr8.4:55284383-55284402 | MS.gene066937:CDS | 45.0% | |
| TACCCTGGCAAGAAAATGAG+CGG | + | chr8.4:55283903-55283922 | MS.gene066937:CDS | 45.0% | |
| ! | TTGGTAACAAGGCTGTTGCT+TGG | + | chr8.4:55284429-55284448 | MS.gene066937:CDS | 45.0% |
| !!! | ATCCCTGTCTGCCATTTTGT+TGG | + | chr8.4:55284410-55284429 | MS.gene066937:CDS | 45.0% |
| GCTTGTCACCATGACACAAG+AGG | + | chr8.4:55284338-55284357 | MS.gene066937:CDS | 50.0% | |
| GTTCCATCAGAGGCCAACAA+TGG | - | chr8.4:55284299-55284318 | None:intergenic | 50.0% | |
| TTGTCTTCTCCGATTGCTGG+TGG | - | chr8.4:55284056-55284075 | None:intergenic | 50.0% | |
| ! | GTTCCATTGTTGGCCTCTGA+TGG | + | chr8.4:55284293-55284312 | MS.gene066937:CDS | 50.0% |
| TGTGGAGGTCCACCAGCAAT+CGG | + | chr8.4:55284044-55284063 | MS.gene066937:CDS | 55.0% | |
| ! | AGGCCGCTCATTTTCTTGCC+AGG | - | chr8.4:55283909-55283928 | None:intergenic | 55.0% |
| ! | GGCCGCTCATTTTCTTGCCA+GGG | - | chr8.4:55283908-55283927 | None:intergenic | 55.0% |
| TGGCAGACAGGGATGGTTCC+AGG | - | chr8.4:55284404-55284423 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 55283846 | 55284502 | 55283846 | ID=MS.gene066937 |
| chr8.4 | mRNA | 55283846 | 55284502 | 55283846 | ID=MS.gene066937.t1;Parent=MS.gene066937 |
| chr8.4 | exon | 55283846 | 55284502 | 55283846 | ID=MS.gene066937.t1.exon1;Parent=MS.gene066937.t1 |
| chr8.4 | CDS | 55283846 | 55284502 | 55283846 | ID=cds.MS.gene066937.t1;Parent=MS.gene066937.t1 |
| Gene Sequence |
| Protein sequence |
