Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58467.t1 | XP_013453015.1 | 92.2 | 231 | 14 | 2 | 1 | 227 | 1 | 231 | 1.70E-100 | 375.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58467.t1 | A0A072UD80 | 92.2 | 231 | 14 | 2 | 1 | 227 | 1 | 231 | 1.2e-100 | 375.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene58467 | 0.80438 | 2.22E-49 | -1.69E-46 |
| MS.gene049419 | MS.gene58467 | -0.813987 | 1.94E-51 | -1.69E-46 |
| MS.gene049680 | MS.gene58467 | 0.804748 | 1.86E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene58467 | 0.802434 | 5.62E-49 | -1.69E-46 |
| MS.gene050571 | MS.gene58467 | 0.801284 | 9.68E-49 | -1.69E-46 |
| MS.gene050752 | MS.gene58467 | 0.816189 | 6.29E-52 | -1.69E-46 |
| MS.gene050945 | MS.gene58467 | 0.821073 | 4.90E-53 | -1.69E-46 |
| MS.gene050955 | MS.gene58467 | 0.810447 | 1.15E-50 | -1.69E-46 |
| MS.gene051343 | MS.gene58467 | 0.847106 | 1.43E-59 | -1.69E-46 |
| MS.gene051676 | MS.gene58467 | 0.869795 | 2.37E-66 | -1.69E-46 |
| MS.gene051831 | MS.gene58467 | 0.80056 | 1.36E-48 | -1.69E-46 |
| MS.gene05188 | MS.gene58467 | 0.827114 | 1.87E-54 | -1.69E-46 |
| MS.gene051902 | MS.gene58467 | 0.823893 | 1.08E-53 | -1.69E-46 |
| MS.gene051903 | MS.gene58467 | 0.808032 | 3.78E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene58467 | 0.881792 | 1.79E-70 | -1.69E-46 |
| MS.gene052072 | MS.gene58467 | 0.804284 | 2.33E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene58467 | 0.804996 | 1.65E-49 | -1.69E-46 |
| MS.gene052533 | MS.gene58467 | 0.807529 | 4.84E-50 | -1.69E-46 |
| MS.gene052889 | MS.gene58467 | 0.810204 | 1.29E-50 | -1.69E-46 |
| MS.gene052947 | MS.gene58467 | 0.810439 | 1.15E-50 | -1.69E-46 |
| MS.gene053058 | MS.gene58467 | 0.808327 | 3.27E-50 | -1.69E-46 |
| MS.gene053094 | MS.gene58467 | 0.839592 | 1.45E-57 | -1.69E-46 |
| MS.gene053189 | MS.gene58467 | 0.814171 | 1.76E-51 | -1.69E-46 |
| MS.gene053298 | MS.gene58467 | 0.826735 | 2.30E-54 | -1.69E-46 |
| MS.gene053340 | MS.gene58467 | 0.856289 | 3.56E-62 | -1.69E-46 |
| MS.gene053564 | MS.gene58467 | 0.84462 | 6.75E-59 | -1.69E-46 |
| MS.gene053952 | MS.gene58467 | 0.803589 | 3.24E-49 | -1.69E-46 |
| MS.gene053963 | MS.gene58467 | 0.835879 | 1.30E-56 | -1.69E-46 |
| MS.gene054222 | MS.gene58467 | 0.821998 | 3.00E-53 | -1.69E-46 |
| MS.gene054403 | MS.gene58467 | 0.818806 | 1.62E-52 | -1.69E-46 |
| MS.gene054536 | MS.gene58467 | 0.822556 | 2.22E-53 | -1.69E-46 |
| MS.gene05469 | MS.gene58467 | 0.943905 | 5.17E-103 | -1.69E-46 |
| MS.gene054892 | MS.gene58467 | 0.804122 | 2.51E-49 | -1.69E-46 |
| MS.gene054893 | MS.gene58467 | 0.805426 | 1.34E-49 | -1.69E-46 |
| MS.gene055147 | MS.gene58467 | 0.819276 | 1.26E-52 | -1.69E-46 |
| MS.gene055148 | MS.gene58467 | 0.812604 | 3.90E-51 | -1.69E-46 |
| MS.gene055311 | MS.gene58467 | 0.825186 | 5.38E-54 | -1.69E-46 |
| MS.gene055313 | MS.gene58467 | 0.832164 | 1.10E-55 | -1.69E-46 |
| MS.gene055314 | MS.gene58467 | 0.822462 | 2.34E-53 | -1.69E-46 |
| MS.gene055603 | MS.gene58467 | 0.840851 | 6.78E-58 | -1.69E-46 |
| MS.gene056403 | MS.gene58467 | 0.808429 | 3.11E-50 | -1.69E-46 |
| MS.gene056605 | MS.gene58467 | 0.807837 | 4.16E-50 | -1.69E-46 |
| MS.gene056638 | MS.gene58467 | 0.800502 | 1.40E-48 | -1.69E-46 |
| MS.gene05689 | MS.gene58467 | 0.807448 | 5.03E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene58467 | 0.884159 | 2.43E-71 | -1.69E-46 |
| MS.gene057102 | MS.gene58467 | 0.828124 | 1.07E-54 | -1.69E-46 |
| MS.gene057890 | MS.gene58467 | -0.830769 | 2.44E-55 | -1.69E-46 |
| MS.gene057999 | MS.gene58467 | 0.847125 | 1.41E-59 | -1.69E-46 |
| MS.gene058159 | MS.gene58467 | 0.800603 | 1.33E-48 | -1.69E-46 |
| MS.gene058592 | MS.gene58467 | 0.804382 | 2.22E-49 | -1.69E-46 |
| MS.gene059146 | MS.gene58467 | 0.828972 | 6.68E-55 | -1.69E-46 |
| MS.gene059227 | MS.gene58467 | 0.816961 | 4.22E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene58467 | -0.851176 | 1.05E-60 | -1.69E-46 |
| MS.gene06001 | MS.gene58467 | 0.807554 | 4.78E-50 | -1.69E-46 |
| MS.gene06011 | MS.gene58467 | 0.856503 | 3.08E-62 | -1.69E-46 |
| MS.gene060244 | MS.gene58467 | 0.814086 | 1.84E-51 | -1.69E-46 |
| MS.gene060365 | MS.gene58467 | 0.839642 | 1.40E-57 | -1.69E-46 |
| MS.gene060763 | MS.gene58467 | 0.81155 | 6.62E-51 | -1.69E-46 |
| MS.gene060937 | MS.gene58467 | 0.808049 | 3.75E-50 | -1.69E-46 |
| MS.gene061188 | MS.gene58467 | 0.813215 | 2.87E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58467.t1 | MTR_6g084600 | 91.775 | 231 | 15 | 2 | 1 | 227 | 1 | 231 | 1.69e-149 | 415 |
| MS.gene58467.t1 | MTR_8g104140 | 39.474 | 228 | 86 | 10 | 5 | 227 | 6 | 186 | 8.56e-27 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58467.t1 | AT1G13360 | 31.100 | 209 | 90 | 8 | 49 | 226 | 8 | 193 | 6.51e-11 | 60.1 |
Find 0 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAACCAAAGTCATCAAAAC+CGG | + | 42699:47190-47209 | None:intergenic | 30.0% | |
| ! | CTTTTCTGATGTTAACTCAT+CGG | - | 42699:46756-46775 | MS.gene58467:CDS | 30.0% |
| ATCAACTCTCTTTCTCTTGT+CGG | + | 42699:46671-46690 | None:intergenic | 35.0% | |
| ATCATCAAACCCAACAAAAC+CGG | + | 42699:47160-47179 | None:intergenic | 35.0% | |
| CGATGAGTTAACATCAGAAA+AGG | + | 42699:46758-46777 | None:intergenic | 35.0% | |
| GAACTTAGGTTACTTGTTTG+AGG | - | 42699:47044-47063 | MS.gene58467:CDS | 35.0% | |
| GTTGTTTGAATATGGTGAAC+CGG | - | 42699:47269-47288 | MS.gene58467:CDS | 35.0% | |
| ! | AAGAAGAGATTTTTGCACCT+GGG | - | 42699:46944-46963 | MS.gene58467:CDS | 35.0% |
| ! | AGTTGATGCTAAGATTCGTA+GGG | - | 42699:46726-46745 | MS.gene58467:CDS | 35.0% |
| !! | GGTTTTGATGACTTTGGTTA+TGG | - | 42699:47189-47208 | MS.gene58467:CDS | 35.0% |
| !!! | GGGTTTGATGATGATTTTAC+CGG | - | 42699:47168-47187 | MS.gene58467:CDS | 35.0% |
| !!! | TTTACCGGTTTTGATGACTT+TGG | - | 42699:47183-47202 | MS.gene58467:CDS | 35.0% |
| GATGACTTTGGTTATGGAAC+TGG | - | 42699:47195-47214 | MS.gene58467:CDS | 40.0% | |
| ! | AGGCTTCTGATGATGAACTT+GGG | - | 42699:47064-47083 | MS.gene58467:CDS | 40.0% |
| ! | CAGTTGATGCTAAGATTCGT+AGG | - | 42699:46725-46744 | MS.gene58467:CDS | 40.0% |
| ! | GAAGAAGAGATTTTTGCACC+TGG | - | 42699:46943-46962 | MS.gene58467:CDS | 40.0% |
| !! | GTCGAGTCAGGTTTTTGATT+CGG | - | 42699:46822-46841 | MS.gene58467:CDS | 40.0% |
| !! | TTATGGAACTGGCTTGTTGT+CGG | - | 42699:47206-47225 | MS.gene58467:CDS | 40.0% |
| AACTCTCTTTCTCTTGTCGG+TGG | + | 42699:46668-46687 | None:intergenic | 45.0% | |
| GGTGAGATGGAGATGAACTT+AGG | - | 42699:47030-47049 | MS.gene58467:CDS | 45.0% | |
| GTTTGAATATGGTGAACCGG+TGG | - | 42699:47272-47291 | MS.gene58467:CDS | 45.0% | |
| ! | GAGGCTTCTGATGATGAACT+TGG | - | 42699:47063-47082 | MS.gene58467:CDS | 45.0% |
| !! | GGGGATGGGTTGTTTGAATA+TGG | - | 42699:47261-47280 | MS.gene58467:CDS | 45.0% |
| ATCCCCCGTCACAAATTCTC+CGG | + | 42699:47247-47266 | None:intergenic | 50.0% | |
| CAAACCCAACAAAACCGGTC+AGG | + | 42699:47155-47174 | None:intergenic | 50.0% | |
| CATCTCCATCTCACCTGAAC+CGG | + | 42699:47025-47044 | None:intergenic | 50.0% | |
| TCAAGACCCATGACTGAGTC+AGG | + | 42699:46904-46923 | None:intergenic | 50.0% | |
| TCGACTCACTCGAGTCAACT+CGG | + | 42699:46807-46826 | None:intergenic | 50.0% | |
| TCTGAGCCTGACTCAGTCAT+GGG | - | 42699:46895-46914 | MS.gene58467:CDS | 50.0% | |
| TGAACCAGGTCTGACTGAAC+CGG | - | 42699:46969-46988 | MS.gene58467:CDS | 50.0% | |
| TGAACCGGGTTCGAATGAAC+CGG | - | 42699:47104-47123 | MS.gene58467:CDS | 50.0% | |
| TTCTGAGCCTGACTCAGTCA+TGG | - | 42699:46894-46913 | MS.gene58467:CDS | 50.0% | |
| TTGTTGTCGGAGAGTGATGG+CGG | - | 42699:47219-47238 | MS.gene58467:CDS | 50.0% | |
| ! | GAGAATTTGTGACGGGGGAT+GGG | - | 42699:47247-47266 | MS.gene58467:CDS | 50.0% |
| !!! | TGGACCTGACCGGTTTTGTT+GGG | - | 42699:47148-47167 | MS.gene58467:CDS | 50.0% |
| ACCGGCCATTTGAACCAGGT+CGG | + | 42699:47007-47026 | None:intergenic | 55.0% | |
| AGGTCTGACTGAACCGGAAC+CGG | - | 42699:46975-46994 | MS.gene58467:CDS | 55.0% | |
| ATATGGTGAACCGGTGGCTG+CGG | - | 42699:47278-47297 | MS.gene58467:CDS | 55.0% | |
| CACAAAACATCCGCAGCCAC+CGG | + | 42699:47291-47310 | None:intergenic | 55.0% | |
| CCACCAACTGTCACTGAACC+GGG | - | 42699:47090-47109 | MS.gene58467:CDS | 55.0% | |
| CGACTCACTCGAGTCAACTC+GGG | + | 42699:46806-46825 | None:intergenic | 55.0% | |
| CGGGTCCGGAGAATTTGTGA+CGG | - | 42699:47239-47258 | MS.gene58467:CDS | 55.0% | |
| CTGAACCGGCCATTTGAACC+AGG | + | 42699:47011-47030 | None:intergenic | 55.0% | |
| GAACCCGGTTCAGTGACAGT+TGG | + | 42699:47096-47115 | None:intergenic | 55.0% | |
| GAACCCGGTTCATTCGAACC+CGG | + | 42699:47111-47130 | None:intergenic | 55.0% | |
| GAACCGGGTTCGAATGAACC+GGG | - | 42699:47105-47124 | MS.gene58467:CDS | 55.0% | |
| GCCACCAACTGTCACTGAAC+CGG | - | 42699:47089-47108 | MS.gene58467:CDS | 55.0% | |
| GGGTCCGGAGAATTTGTGAC+GGG | - | 42699:47240-47259 | MS.gene58467:CDS | 55.0% | |
| GGGTTCGAATGAACCGGGTT+CGG | - | 42699:47110-47129 | MS.gene58467:CDS | 55.0% | |
| GGTCCGGAGAATTTGTGACG+GGG | - | 42699:47241-47260 | MS.gene58467:CDS | 55.0% | |
| GTCCGGAGAATTTGTGACGG+GGG | - | 42699:47242-47261 | MS.gene58467:CDS | 55.0% | |
| TCAGGCCGACCTGGTTCAAA+TGG | - | 42699:46999-47018 | MS.gene58467:CDS | 55.0% | |
| TGACTCGAGTGAGTCGAGTC+AGG | - | 42699:46810-46829 | MS.gene58467:CDS | 55.0% | |
| TGTTGTCGGAGAGTGATGGC+GGG | - | 42699:47220-47239 | MS.gene58467:CDS | 55.0% | |
| TTCGGTTGAACCGGAGAAGG+TGG | - | 42699:47128-47147 | MS.gene58467:CDS | 55.0% | |
| TTCTCCGGTTCAACCGAACC+CGG | + | 42699:47126-47145 | None:intergenic | 55.0% | |
| TTTGCACCTGGGACTGAACC+AGG | - | 42699:46955-46974 | MS.gene58467:CDS | 55.0% | |
| ! | AATGGCCGGTTCAGGTGAGA+TGG | - | 42699:47017-47036 | MS.gene58467:CDS | 55.0% |
| ! | CTGGTTCAAATGGCCGGTTC+AGG | - | 42699:47009-47028 | MS.gene58467:CDS | 55.0% |
| ! | GGAGAATTTGTGACGGGGGA+TGG | - | 42699:47246-47265 | MS.gene58467:CDS | 55.0% |
| ! | TGAACCGGGTTCGGTTGAAC+CGG | - | 42699:47119-47138 | MS.gene58467:CDS | 55.0% |
| ! | TGCGGATGTTTTGTGGCGGT+CGG | - | 42699:47296-47315 | MS.gene58467:CDS | 55.0% |
| ! | TGGCTGCGGATGTTTTGTGG+CGG | - | 42699:47292-47311 | MS.gene58467:CDS | 55.0% |
| !! | GGCTTGTTGTCGGAGAGTGA+TGG | - | 42699:47216-47235 | MS.gene58467:CDS | 55.0% |
| !! | GTGGACCTGACCGGTTTTGT+TGG | - | 42699:47147-47166 | MS.gene58467:CDS | 55.0% |
| CCCGGTTCAGTGACAGTTGG+TGG | + | 42699:47093-47112 | None:intergenic | 60.0% | |
| GCCGACCTGGTTCAAATGGC+CGG | - | 42699:47003-47022 | MS.gene58467:CDS | 60.0% | |
| GGTTCCGGTTCAGTCAGACC+TGG | + | 42699:46976-46995 | None:intergenic | 60.0% | |
| GTCAGACCTGGTTCAGTCCC+AGG | + | 42699:46964-46983 | None:intergenic | 60.0% | |
| TGAACCAGGTCGGCCTGAAC+CGG | + | 42699:46997-47016 | None:intergenic | 60.0% | |
| ! | CGGTGGCTGCGGATGTTTTG+TGG | - | 42699:47289-47308 | MS.gene58467:CDS | 60.0% |
| ! | GACTGAACCGGAACCGGTTC+AGG | - | 42699:46981-47000 | MS.gene58467:CDS | 60.0% |
| ! | GGGTTCGGTTGAACCGGAGA+AGG | - | 42699:47125-47144 | MS.gene58467:CDS | 60.0% |
| CCGGAGAAGGTGGACCTGAC+CGG | - | 42699:47138-47157 | MS.gene58467:CDS | 65.0% | |
| CCGGTCAGGTCCACCTTCTC+CGG | + | 42699:47141-47160 | None:intergenic | 65.0% | |
| ! | AGGTCGGCCTGAACCGGTTC+CGG | + | 42699:46991-47010 | None:intergenic | 65.0% |
| !! | TCGGAGAGTGATGGCGGGTC+CGG | - | 42699:47225-47244 | MS.gene58467:CDS | 65.0% |
| ! | GGAACCGGTTCAGGCCGACC+TGG | - | 42699:46990-47009 | MS.gene58467:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42699 | gene | 46655 | 47338 | 46655 | ID=MS.gene58467 |
| 42699 | mRNA | 46655 | 47338 | 46655 | ID=MS.gene58467.t1;Parent=MS.gene58467 |
| 42699 | exon | 46655 | 47338 | 46655 | ID=MS.gene58467.t1.exon1;Parent=MS.gene58467.t1 |
| 42699 | CDS | 46655 | 47338 | 46655 | ID=cds.MS.gene58467.t1;Parent=MS.gene58467.t1 |
| Gene Sequence |
| Protein sequence |
