Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22433.t1 | XP_003625632.1 | 93.4 | 166 | 5 | 2 | 1 | 166 | 1 | 160 | 1.00E-70 | 276.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22433.t1 | Q2HV51 | 93.4 | 166 | 5 | 2 | 1 | 166 | 1 | 160 | 7.3e-71 | 276.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene22433 | 0.806342 | 8.62E-50 | -1.69E-46 |
| MS.gene04919 | MS.gene22433 | 0.808562 | 2.91E-50 | -1.69E-46 |
| MS.gene04920 | MS.gene22433 | 0.819894 | 9.14E-53 | -1.69E-46 |
| MS.gene049283 | MS.gene22433 | 0.849058 | 4.12E-60 | -1.69E-46 |
| MS.gene049499 | MS.gene22433 | 0.813787 | 2.14E-51 | -1.69E-46 |
| MS.gene049983 | MS.gene22433 | 0.823021 | 1.73E-53 | -1.69E-46 |
| MS.gene04998 | MS.gene22433 | 0.816953 | 4.24E-52 | -1.69E-46 |
| MS.gene050359 | MS.gene22433 | 0.804614 | 1.98E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene22433 | 0.827255 | 1.73E-54 | -1.69E-46 |
| MS.gene050646 | MS.gene22433 | 0.838008 | 3.71E-57 | -1.69E-46 |
| MS.gene050752 | MS.gene22433 | 0.834594 | 2.74E-56 | -1.69E-46 |
| MS.gene050838 | MS.gene22433 | 0.806275 | 8.90E-50 | -1.69E-46 |
| MS.gene050945 | MS.gene22433 | 0.8576 | 1.46E-62 | -1.69E-46 |
| MS.gene050955 | MS.gene22433 | 0.834017 | 3.82E-56 | -1.69E-46 |
| MS.gene051003 | MS.gene22433 | 0.812373 | 4.38E-51 | -1.69E-46 |
| MS.gene051043 | MS.gene22433 | 0.828834 | 7.21E-55 | -1.69E-46 |
| MS.gene051226 | MS.gene22433 | 0.806877 | 6.65E-50 | -1.69E-46 |
| MS.gene051234 | MS.gene22433 | 0.82036 | 7.15E-53 | -1.69E-46 |
| MS.gene051343 | MS.gene22433 | 0.843419 | 1.42E-58 | -1.69E-46 |
| MS.gene051676 | MS.gene22433 | 0.805844 | 1.10E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene22433 | 0.82425 | 8.93E-54 | -1.69E-46 |
| MS.gene051856 | MS.gene22433 | 0.811723 | 6.07E-51 | -1.69E-46 |
| MS.gene051902 | MS.gene22433 | 0.847935 | 8.44E-60 | -1.69E-46 |
| MS.gene051975 | MS.gene22433 | 0.806024 | 1.01E-49 | -1.69E-46 |
| MS.gene052016 | MS.gene22433 | 0.804919 | 1.71E-49 | -1.69E-46 |
| MS.gene052072 | MS.gene22433 | 0.819342 | 1.22E-52 | -1.69E-46 |
| MS.gene052239 | MS.gene22433 | -0.809496 | 1.84E-50 | -1.69E-46 |
| MS.gene052242 | MS.gene22433 | -0.828681 | 7.85E-55 | -1.69E-46 |
| MS.gene052296 | MS.gene22433 | 0.8319 | 1.28E-55 | -1.69E-46 |
| MS.gene052479 | MS.gene22433 | 0.8019 | 7.24E-49 | -1.69E-46 |
| MS.gene052533 | MS.gene22433 | 0.806289 | 8.84E-50 | -1.69E-46 |
| MS.gene052793 | MS.gene22433 | 0.83709 | 6.38E-57 | -1.69E-46 |
| MS.gene053045 | MS.gene22433 | 0.824065 | 9.88E-54 | -1.69E-46 |
| MS.gene053189 | MS.gene22433 | 0.846305 | 2.36E-59 | -1.69E-46 |
| MS.gene053298 | MS.gene22433 | 0.80991 | 1.50E-50 | -1.69E-46 |
| MS.gene053564 | MS.gene22433 | 0.829012 | 6.53E-55 | -1.69E-46 |
| MS.gene053879 | MS.gene22433 | 0.805505 | 1.29E-49 | -1.69E-46 |
| MS.gene053931 | MS.gene22433 | 0.820467 | 6.75E-53 | -1.69E-46 |
| MS.gene053963 | MS.gene22433 | 0.817838 | 2.68E-52 | -1.69E-46 |
| MS.gene054004 | MS.gene22433 | 0.804229 | 2.39E-49 | -1.69E-46 |
| MS.gene054178 | MS.gene22433 | 0.802813 | 4.69E-49 | -1.69E-46 |
| MS.gene054222 | MS.gene22433 | 0.818268 | 2.14E-52 | -1.69E-46 |
| MS.gene054403 | MS.gene22433 | 0.839285 | 1.74E-57 | -1.69E-46 |
| MS.gene054413 | MS.gene22433 | 0.815214 | 1.04E-51 | -1.69E-46 |
| MS.gene054536 | MS.gene22433 | 0.8044 | 2.20E-49 | -1.69E-46 |
| MS.gene05469 | MS.gene22433 | 0.806576 | 7.69E-50 | -1.69E-46 |
| MS.gene054709 | MS.gene22433 | 0.803491 | 3.40E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene22433 | 0.814654 | 1.38E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene22433 | 0.818677 | 1.73E-52 | -1.69E-46 |
| MS.gene054957 | MS.gene22433 | 0.801914 | 7.19E-49 | -1.69E-46 |
| MS.gene055841 | MS.gene22433 | 0.808639 | 2.81E-50 | -1.69E-46 |
| MS.gene056082 | MS.gene22433 | 0.845545 | 3.80E-59 | -1.69E-46 |
| MS.gene056699 | MS.gene22433 | 0.800053 | 1.73E-48 | -1.69E-46 |
| MS.gene057102 | MS.gene22433 | 0.8432 | 1.62E-58 | -1.69E-46 |
| MS.gene057155 | MS.gene22433 | 0.801905 | 7.22E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene22433 | 0.815958 | 7.08E-52 | -1.69E-46 |
| MS.gene057622 | MS.gene22433 | 0.816755 | 4.70E-52 | -1.69E-46 |
| MS.gene057694 | MS.gene22433 | 0.81244 | 4.24E-51 | -1.69E-46 |
| MS.gene057705 | MS.gene22433 | 0.825373 | 4.86E-54 | -1.69E-46 |
| MS.gene057890 | MS.gene22433 | -0.826182 | 3.12E-54 | -1.69E-46 |
| MS.gene057999 | MS.gene22433 | 0.826391 | 2.78E-54 | -1.69E-46 |
| MS.gene058008 | MS.gene22433 | 0.811897 | 5.56E-51 | -1.69E-46 |
| MS.gene058074 | MS.gene22433 | 0.823361 | 1.44E-53 | -1.69E-46 |
| MS.gene058159 | MS.gene22433 | 0.806654 | 7.41E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene22433 | 0.81488 | 1.23E-51 | -1.69E-46 |
| MS.gene058279 | MS.gene22433 | 0.813244 | 2.82E-51 | -1.69E-46 |
| MS.gene058592 | MS.gene22433 | 0.82308 | 1.68E-53 | -1.69E-46 |
| MS.gene059146 | MS.gene22433 | 0.818399 | 2.00E-52 | -1.69E-46 |
| MS.gene059170 | MS.gene22433 | 0.809752 | 1.62E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene22433 | 0.823891 | 1.08E-53 | -1.69E-46 |
| MS.gene05985 | MS.gene22433 | -0.842776 | 2.10E-58 | -1.69E-46 |
| MS.gene06018 | MS.gene22433 | 0.817393 | 3.38E-52 | -1.69E-46 |
| MS.gene060244 | MS.gene22433 | 0.850625 | 1.50E-60 | -1.69E-46 |
| MS.gene060259 | MS.gene22433 | 0.824566 | 7.53E-54 | -1.69E-46 |
| MS.gene060365 | MS.gene22433 | 0.827282 | 1.71E-54 | -1.69E-46 |
| MS.gene060933 | MS.gene22433 | 0.805136 | 1.54E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene22433 | 0.807664 | 4.53E-50 | -1.69E-46 |
| MS.gene060937 | MS.gene22433 | 0.81828 | 2.13E-52 | -1.69E-46 |
| MS.gene061100 | MS.gene22433 | 0.835297 | 1.82E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22433.t1 | MTR_7g101250 | 94.578 | 166 | 3 | 2 | 1 | 166 | 1 | 160 | 4.30e-111 | 313 |
| MS.gene22433.t1 | MTR_2g094410 | 43.312 | 157 | 73 | 5 | 11 | 166 | 15 | 156 | 4.52e-31 | 110 |
| MS.gene22433.t1 | MTR_4g059320 | 47.368 | 133 | 62 | 4 | 36 | 165 | 31 | 158 | 2.20e-30 | 108 |
| MS.gene22433.t1 | MTR_4g132020 | 35.450 | 189 | 84 | 8 | 1 | 165 | 1 | 175 | 1.19e-21 | 86.7 |
| MS.gene22433.t1 | MTR_2g023070 | 47.059 | 85 | 40 | 2 | 82 | 165 | 137 | 217 | 1.97e-17 | 76.3 |
| MS.gene22433.t1 | MTR_2g023390 | 51.163 | 86 | 34 | 2 | 83 | 164 | 73 | 154 | 5.83e-16 | 71.2 |
| MS.gene22433.t1 | MTR_2g086660 | 40.230 | 87 | 47 | 2 | 84 | 166 | 170 | 255 | 4.96e-12 | 62.4 |
| MS.gene22433.t1 | MTR_3g052330 | 39.091 | 110 | 38 | 4 | 84 | 164 | 87 | 196 | 1.41e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22433.t1 | AT5G03230 | 40.805 | 174 | 84 | 5 | 3 | 166 | 2 | 166 | 3.21e-32 | 113 |
| MS.gene22433.t1 | AT3G45210 | 43.636 | 165 | 74 | 4 | 2 | 166 | 3 | 148 | 1.20e-29 | 106 |
| MS.gene22433.t1 | AT2G28400 | 38.824 | 170 | 92 | 3 | 1 | 166 | 1 | 162 | 9.39e-28 | 102 |
| MS.gene22433.t1 | AT5G60680 | 43.023 | 172 | 74 | 5 | 6 | 166 | 5 | 163 | 1.50e-27 | 101 |
| MS.gene22433.t1 | AT4G21970 | 49.462 | 93 | 28 | 4 | 83 | 164 | 59 | 143 | 2.14e-17 | 75.1 |
| MS.gene22433.t1 | AT4G04630 | 38.298 | 141 | 74 | 4 | 37 | 164 | 26 | 166 | 7.36e-17 | 73.9 |
| MS.gene22433.t1 | AT1G11700 | 43.750 | 96 | 44 | 2 | 76 | 164 | 107 | 199 | 1.42e-15 | 71.2 |
| MS.gene22433.t1 | AT1G61930 | 46.154 | 91 | 39 | 2 | 82 | 164 | 113 | 201 | 4.93e-15 | 69.7 |
| MS.gene22433.t1 | AT4G26950 | 36.170 | 141 | 64 | 5 | 37 | 164 | 15 | 142 | 3.28e-14 | 66.2 |
| MS.gene22433.t1 | AT4G26950 | 36.170 | 141 | 64 | 5 | 37 | 164 | 15 | 142 | 3.28e-14 | 66.2 |
| MS.gene22433.t1 | AT4G21930 | 35.897 | 156 | 66 | 8 | 35 | 164 | 34 | 181 | 9.15e-14 | 66.2 |
| MS.gene22433.t1 | AT3G15040 | 41.379 | 87 | 47 | 1 | 84 | 166 | 157 | 243 | 2.29e-12 | 63.2 |
| MS.gene22433.t1 | AT1G29640 | 41.284 | 109 | 43 | 5 | 65 | 164 | 41 | 137 | 1.11e-11 | 59.7 |
Find 29 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCAATCAATGGCTTCTTCT+AGG | 0.289420 | 7.1:+8622200 | None:intergenic |
| GTATTCATCCTTCAAAATCT+TGG | 0.290307 | 7.1:-8622485 | None:intergenic |
| GGGAAAGGAAGGACTTTAAA+AGG | 0.299267 | 7.1:+8622628 | MS.gene22433:CDS |
| GTAGTGTAAGGAATGCTATT+TGG | 0.358519 | 7.1:+8622662 | MS.gene22433:CDS |
| ATGCTATTTGGAAGAAAGTT+GGG | 0.379786 | 7.1:+8622674 | MS.gene22433:CDS |
| GACCAATCTGGTATATTCAT+TGG | 0.380128 | 7.1:-8622463 | None:intergenic |
| AATGCTATTTGGAAGAAAGT+TGG | 0.385752 | 7.1:+8622673 | MS.gene22433:CDS |
| TTCAAAATCTTGGACCAATC+TGG | 0.413413 | 7.1:-8622475 | None:intergenic |
| CTAGAAACTCTCTTCAAACC+TGG | 0.465204 | 7.1:-8622391 | None:intergenic |
| GTTTCTGTCTCTGAGGTAGT+TGG | 0.466042 | 7.1:-8622256 | None:intergenic |
| AGTTTGATGAAGCTGATGTA+TGG | 0.474055 | 7.1:+8622317 | MS.gene22433:CDS |
| GAAGAGACTTGCGTAGTGTA+AGG | 0.480322 | 7.1:+8622650 | MS.gene22433:CDS |
| AAATGTAACTTGTTCTTGAA+AGG | 0.483499 | 7.1:-8622231 | None:intergenic |
| CTTTCTGTTCATGAAGGGAA+AGG | 0.500406 | 7.1:+8622613 | MS.gene22433:CDS |
| GAAGATGAAGGTGATTATGA+TGG | 0.512537 | 7.1:+8622535 | MS.gene22433:CDS |
| GCTTCTCTTTCTGTTCATGA+AGG | 0.513560 | 7.1:+8622607 | MS.gene22433:CDS |
| CTTCTCTTTCTGTTCATGAA+GGG | 0.517982 | 7.1:+8622608 | MS.gene22433:CDS |
| TACCAATGAATATACCAGAT+TGG | 0.518610 | 7.1:+8622461 | MS.gene22433:CDS |
| AGTAGTGATGATGAAGATGA+AGG | 0.546106 | 7.1:+8622523 | MS.gene22433:CDS |
| GGTAATGAAGATGAAGCTAA+AGG | 0.550449 | 7.1:-8622442 | None:intergenic |
| CTGTTCATGAAGGGAAAGGA+AGG | 0.560984 | 7.1:+8622617 | MS.gene22433:CDS |
| GAATATCTTCTTAGAACTAG+AGG | 0.587771 | 7.1:+8622583 | MS.gene22433:CDS |
| TGAAGGTGATTATGATGGAG+GGG | 0.591476 | 7.1:+8622540 | MS.gene22433:CDS |
| GAAATTTGTTTCTGTCTCTG+AGG | 0.611033 | 7.1:-8622263 | None:intergenic |
| GATGAAGGTGATTATGATGG+AGG | 0.617604 | 7.1:+8622538 | MS.gene22433:CDS |
| ATGAAGGTGATTATGATGGA+GGG | 0.625231 | 7.1:+8622539 | MS.gene22433:CDS |
| CTAAGAAGATATTCATGAGG+AGG | 0.629545 | 7.1:-8622574 | None:intergenic |
| AGGTGATTATGATGGAGGGG+TGG | 0.659515 | 7.1:+8622543 | MS.gene22433:CDS |
| GTTCTAAGAAGATATTCATG+AGG | 0.678087 | 7.1:-8622577 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCAATCAAAAATCATCACA+AGG | + | chr7.1:8622283-8622302 | MS.gene22433:CDS | 25.0% |
| !!! | AAATGTAACTTGTTCTTGAA+AGG | - | chr7.1:8622234-8622253 | None:intergenic | 25.0% |
| ACAAACATAGAGCCAAAAAA+GGG | + | chr7.1:8622364-8622383 | MS.gene22433:CDS | 30.0% | |
| GAATATCTTCTTAGAACTAG+AGG | + | chr7.1:8622583-8622602 | MS.gene22433:CDS | 30.0% | |
| GTATTCATCCTTCAAAATCT+TGG | - | chr7.1:8622488-8622507 | None:intergenic | 30.0% | |
| TACCAATGAATATACCAGAT+TGG | + | chr7.1:8622461-8622480 | MS.gene22433:CDS | 30.0% | |
| ! | AATGCTATTTGGAAGAAAGT+TGG | + | chr7.1:8622673-8622692 | MS.gene22433:CDS | 30.0% |
| ! | ATGCTATTTGGAAGAAAGTT+GGG | + | chr7.1:8622674-8622693 | MS.gene22433:CDS | 30.0% |
| ! | GAAGAGAGTTTCTAGAAAAA+TGG | + | chr7.1:8622399-8622418 | MS.gene22433:CDS | 30.0% |
| !! | GTTCTAAGAAGATATTCATG+AGG | - | chr7.1:8622580-8622599 | None:intergenic | 30.0% |
| AGTTTGATGAAGCTGATGTA+TGG | + | chr7.1:8622317-8622336 | MS.gene22433:CDS | 35.0% | |
| CACAAACATAGAGCCAAAAA+AGG | + | chr7.1:8622363-8622382 | MS.gene22433:CDS | 35.0% | |
| CTAAGAAGATATTCATGAGG+AGG | - | chr7.1:8622577-8622596 | None:intergenic | 35.0% | |
| CTTCTCTTTCTGTTCATGAA+GGG | + | chr7.1:8622608-8622627 | MS.gene22433:CDS | 35.0% | |
| GAAGATGAAGGTGATTATGA+TGG | + | chr7.1:8622535-8622554 | MS.gene22433:CDS | 35.0% | |
| GACCAATCTGGTATATTCAT+TGG | - | chr7.1:8622466-8622485 | None:intergenic | 35.0% | |
| GGTAATGAAGATGAAGCTAA+AGG | - | chr7.1:8622445-8622464 | None:intergenic | 35.0% | |
| GTAGTGTAAGGAATGCTATT+TGG | + | chr7.1:8622662-8622681 | MS.gene22433:CDS | 35.0% | |
| TTCAAAATCTTGGACCAATC+TGG | - | chr7.1:8622478-8622497 | None:intergenic | 35.0% | |
| ! | AGTAGTGATGATGAAGATGA+AGG | + | chr7.1:8622523-8622542 | MS.gene22433:CDS | 35.0% |
| ! | ATGAAGGTGATTATGATGGA+GGG | + | chr7.1:8622539-8622558 | MS.gene22433:CDS | 35.0% |
| !! | AGATTGGTCCAAGATTTTGA+AGG | + | chr7.1:8622477-8622496 | MS.gene22433:CDS | 35.0% |
| !! | GAAATTTGTTTCTGTCTCTG+AGG | - | chr7.1:8622266-8622285 | None:intergenic | 35.0% |
| !!! | TTTTGGCTCTATGTTTGTGT+TGG | - | chr7.1:8622362-8622381 | None:intergenic | 35.0% |
| CTAGAAACTCTCTTCAAACC+TGG | - | chr7.1:8622394-8622413 | None:intergenic | 40.0% | |
| CTTTCTGTTCATGAAGGGAA+AGG | + | chr7.1:8622613-8622632 | MS.gene22433:CDS | 40.0% | |
| GATGAAGGTGATTATGATGG+AGG | + | chr7.1:8622538-8622557 | MS.gene22433:CDS | 40.0% | |
| GCTTCTCTTTCTGTTCATGA+AGG | + | chr7.1:8622607-8622626 | MS.gene22433:CDS | 40.0% | |
| GGGAAAGGAAGGACTTTAAA+AGG | + | chr7.1:8622628-8622647 | MS.gene22433:CDS | 40.0% | |
| ! | TGAAGGTGATTATGATGGAG+GGG | + | chr7.1:8622540-8622559 | MS.gene22433:CDS | 40.0% |
| !!! | AAACCTGGTACACCCTTTTT+TGG | - | chr7.1:8622379-8622398 | None:intergenic | 40.0% |
| CTGTTCATGAAGGGAAAGGA+AGG | + | chr7.1:8622617-8622636 | MS.gene22433:CDS | 45.0% | |
| GAAGAGACTTGCGTAGTGTA+AGG | + | chr7.1:8622650-8622669 | MS.gene22433:CDS | 45.0% | |
| !! | GTTTCTGTCTCTGAGGTAGT+TGG | - | chr7.1:8622259-8622278 | None:intergenic | 45.0% |
| ! | AGGTGATTATGATGGAGGGG+TGG | + | chr7.1:8622543-8622562 | MS.gene22433:CDS | 50.0% |
| ! | GAGCCAAAAAAGGGTGTACC+AGG | + | chr7.1:8622373-8622392 | MS.gene22433:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 8622208 | 8622708 | 8622208 | ID=MS.gene22433 |
| chr7.1 | mRNA | 8622208 | 8622708 | 8622208 | ID=MS.gene22433.t1;Parent=MS.gene22433 |
| chr7.1 | exon | 8622208 | 8622708 | 8622208 | ID=MS.gene22433.t1.exon1;Parent=MS.gene22433.t1 |
| chr7.1 | CDS | 8622208 | 8622708 | 8622208 | ID=cds.MS.gene22433.t1;Parent=MS.gene22433.t1 |
| Gene Sequence |
| Protein sequence |
