Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51920.t1 | XP_003624559.1 | 98.9 | 436 | 4 | 1 | 1 | 436 | 1 | 435 | 6.20E-245 | 856.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51920.t1 | P35614 | 91.5 | 436 | 36 | 1 | 1 | 436 | 1 | 435 | 2.6e-231 | 802.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51920.t1 | G7KYC6 | 98.9 | 436 | 4 | 1 | 1 | 436 | 1 | 435 | 4.5e-245 | 856.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene51920 | 0.815332 | 9.76E-52 | -1.69E-46 |
| MS.gene049208 | MS.gene51920 | 0.81349 | 2.49E-51 | -1.69E-46 |
| MS.gene049861 | MS.gene51920 | 0.811419 | 7.07E-51 | -1.69E-46 |
| MS.gene050084 | MS.gene51920 | 0.810073 | 1.38E-50 | -1.69E-46 |
| MS.gene050085 | MS.gene51920 | 0.835564 | 1.56E-56 | -1.69E-46 |
| MS.gene050114 | MS.gene51920 | 0.876752 | 1.09E-68 | -1.69E-46 |
| MS.gene050277 | MS.gene51920 | 0.817446 | 3.28E-52 | -1.69E-46 |
| MS.gene050288 | MS.gene51920 | 0.810362 | 1.20E-50 | -1.69E-46 |
| MS.gene050317 | MS.gene51920 | 0.846513 | 2.07E-59 | -1.69E-46 |
| MS.gene050320 | MS.gene51920 | 0.813238 | 2.83E-51 | -1.69E-46 |
| MS.gene050359 | MS.gene51920 | 0.810127 | 1.35E-50 | -1.69E-46 |
| MS.gene050752 | MS.gene51920 | 0.831249 | 1.86E-55 | -1.69E-46 |
| MS.gene050838 | MS.gene51920 | 0.901595 | 2.29E-78 | -1.69E-46 |
| MS.gene050955 | MS.gene51920 | 0.8046 | 2.00E-49 | -1.69E-46 |
| MS.gene051003 | MS.gene51920 | 0.859958 | 2.88E-63 | -1.69E-46 |
| MS.gene051063 | MS.gene51920 | 0.805306 | 1.42E-49 | -1.69E-46 |
| MS.gene051233 | MS.gene51920 | 0.816689 | 4.86E-52 | -1.69E-46 |
| MS.gene051234 | MS.gene51920 | 0.829269 | 5.66E-55 | -1.69E-46 |
| MS.gene051245 | MS.gene51920 | 0.820788 | 5.70E-53 | -1.69E-46 |
| MS.gene051343 | MS.gene51920 | 0.846701 | 1.84E-59 | -1.69E-46 |
| MS.gene051381 | MS.gene51920 | 0.814891 | 1.22E-51 | -1.69E-46 |
| MS.gene051746 | MS.gene51920 | 0.849829 | 2.52E-60 | -1.69E-46 |
| MS.gene051748 | MS.gene51920 | 0.863163 | 3.03E-64 | -1.69E-46 |
| MS.gene051756 | MS.gene51920 | 0.863398 | 2.56E-64 | -1.69E-46 |
| MS.gene051757 | MS.gene51920 | 0.811731 | 6.04E-51 | -1.69E-46 |
| MS.gene051850 | MS.gene51920 | 0.81975 | 9.86E-53 | -1.69E-46 |
| MS.gene05188 | MS.gene51920 | 0.836412 | 9.50E-57 | -1.69E-46 |
| MS.gene051902 | MS.gene51920 | 0.801449 | 8.96E-49 | -1.69E-46 |
| MS.gene051903 | MS.gene51920 | 0.851264 | 9.94E-61 | -1.69E-46 |
| MS.gene051975 | MS.gene51920 | 0.868154 | 8.06E-66 | -1.69E-46 |
| MS.gene052072 | MS.gene51920 | 0.801302 | 9.60E-49 | -1.69E-46 |
| MS.gene052335 | MS.gene51920 | 0.811375 | 7.23E-51 | -1.69E-46 |
| MS.gene052342 | MS.gene51920 | 0.809702 | 1.66E-50 | -1.69E-46 |
| MS.gene052370 | MS.gene51920 | 0.807049 | 6.11E-50 | -1.69E-46 |
| MS.gene052434 | MS.gene51920 | 0.837031 | 6.61E-57 | -1.69E-46 |
| MS.gene052479 | MS.gene51920 | 0.805525 | 1.28E-49 | -1.69E-46 |
| MS.gene052932 | MS.gene51920 | 0.801044 | 1.08E-48 | -1.69E-46 |
| MS.gene052947 | MS.gene51920 | 0.828437 | 9.00E-55 | -1.69E-46 |
| MS.gene053058 | MS.gene51920 | 0.878467 | 2.74E-69 | -1.69E-46 |
| MS.gene053109 | MS.gene51920 | 0.839119 | 1.92E-57 | -1.69E-46 |
| MS.gene053381 | MS.gene51920 | 0.838418 | 2.91E-57 | -1.69E-46 |
| MS.gene053564 | MS.gene51920 | 0.839289 | 1.73E-57 | -1.69E-46 |
| MS.gene053786 | MS.gene51920 | 0.824616 | 7.33E-54 | -1.69E-46 |
| MS.gene05380 | MS.gene51920 | 0.805414 | 1.35E-49 | -1.69E-46 |
| MS.gene053952 | MS.gene51920 | 0.827017 | 1.97E-54 | -1.69E-46 |
| MS.gene054403 | MS.gene51920 | 0.828153 | 1.05E-54 | -1.69E-46 |
| MS.gene054536 | MS.gene51920 | 0.808775 | 2.62E-50 | -1.69E-46 |
| MS.gene05469 | MS.gene51920 | 0.835208 | 1.92E-56 | -1.69E-46 |
| MS.gene055311 | MS.gene51920 | 0.851937 | 6.41E-61 | -1.69E-46 |
| MS.gene055313 | MS.gene51920 | 0.826355 | 2.84E-54 | -1.69E-46 |
| MS.gene055314 | MS.gene51920 | 0.843837 | 1.10E-58 | -1.69E-46 |
| MS.gene055424 | MS.gene51920 | 0.83471 | 2.56E-56 | -1.69E-46 |
| MS.gene055513 | MS.gene51920 | 0.800775 | 1.23E-48 | -1.69E-46 |
| MS.gene055603 | MS.gene51920 | 0.849848 | 2.48E-60 | -1.69E-46 |
| MS.gene056097 | MS.gene51920 | 0.849733 | 2.68E-60 | -1.69E-46 |
| MS.gene056181 | MS.gene51920 | 0.831938 | 1.26E-55 | -1.69E-46 |
| MS.gene056403 | MS.gene51920 | 0.837803 | 4.19E-57 | -1.69E-46 |
| MS.gene056580 | MS.gene51920 | 0.818695 | 1.71E-52 | -1.69E-46 |
| MS.gene056605 | MS.gene51920 | 0.844695 | 6.45E-59 | -1.69E-46 |
| MS.gene056631 | MS.gene51920 | 0.819018 | 1.45E-52 | -1.69E-46 |
| MS.gene056752 | MS.gene51920 | 0.811424 | 7.05E-51 | -1.69E-46 |
| MS.gene056787 | MS.gene51920 | 0.874556 | 6.16E-68 | -1.69E-46 |
| MS.gene056849 | MS.gene51920 | 0.812847 | 3.45E-51 | -1.69E-46 |
| MS.gene056896 | MS.gene51920 | 0.807201 | 5.67E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene51920 | 0.87015 | 1.81E-66 | -1.69E-46 |
| MS.gene056992 | MS.gene51920 | 0.840381 | 9.00E-58 | -1.69E-46 |
| MS.gene057057 | MS.gene51920 | 0.818164 | 2.26E-52 | -1.69E-46 |
| MS.gene057094 | MS.gene51920 | 0.886151 | 4.39E-72 | -1.69E-46 |
| MS.gene057144 | MS.gene51920 | 0.806618 | 7.54E-50 | -1.69E-46 |
| MS.gene057145 | MS.gene51920 | 0.809552 | 1.79E-50 | -1.69E-46 |
| MS.gene057234 | MS.gene51920 | 0.837768 | 4.28E-57 | -1.69E-46 |
| MS.gene057360 | MS.gene51920 | 0.807909 | 4.01E-50 | -1.69E-46 |
| MS.gene057452 | MS.gene51920 | -0.840905 | 6.56E-58 | -1.69E-46 |
| MS.gene05781 | MS.gene51920 | 0.83049 | 2.85E-55 | -1.69E-46 |
| MS.gene057890 | MS.gene51920 | -0.817173 | 3.78E-52 | -1.69E-46 |
| MS.gene057999 | MS.gene51920 | 0.854915 | 8.95E-62 | -1.69E-46 |
| MS.gene058054 | MS.gene51920 | 0.813268 | 2.79E-51 | -1.69E-46 |
| MS.gene058055 | MS.gene51920 | 0.816278 | 6.01E-52 | -1.69E-46 |
| MS.gene058074 | MS.gene51920 | 0.837954 | 3.83E-57 | -1.69E-46 |
| MS.gene058238 | MS.gene51920 | 0.857635 | 1.43E-62 | -1.69E-46 |
| MS.gene058639 | MS.gene51920 | 0.804646 | 1.96E-49 | -1.69E-46 |
| MS.gene058654 | MS.gene51920 | 0.829417 | 5.21E-55 | -1.69E-46 |
| MS.gene058758 | MS.gene51920 | 0.84836 | 6.44E-60 | -1.69E-46 |
| MS.gene058906 | MS.gene51920 | 0.800282 | 1.55E-48 | -1.69E-46 |
| MS.gene059007 | MS.gene51920 | 0.801667 | 8.08E-49 | -1.69E-46 |
| MS.gene059050 | MS.gene51920 | 0.80901 | 2.34E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene51920 | 0.848254 | 6.89E-60 | -1.69E-46 |
| MS.gene059171 | MS.gene51920 | 0.839559 | 1.47E-57 | -1.69E-46 |
| MS.gene059360 | MS.gene51920 | 0.82627 | 2.97E-54 | -1.69E-46 |
| MS.gene06001 | MS.gene51920 | 0.841 | 6.20E-58 | -1.69E-46 |
| MS.gene06010 | MS.gene51920 | 0.838033 | 3.66E-57 | -1.69E-46 |
| MS.gene06011 | MS.gene51920 | 0.886261 | 3.99E-72 | -1.69E-46 |
| MS.gene060465 | MS.gene51920 | 0.80543 | 1.34E-49 | -1.69E-46 |
| MS.gene060702 | MS.gene51920 | 0.802488 | 5.48E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene51920 | 0.842982 | 1.85E-58 | -1.69E-46 |
| MS.gene060869 | MS.gene51920 | 0.840592 | 7.93E-58 | -1.69E-46 |
| MS.gene060870 | MS.gene51920 | 0.816658 | 4.94E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene51920 | MS.gene52281 | PPI |
| MS.gene41681 | MS.gene51920 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51920.t1 | MTR_7g084860 | 98.853 | 436 | 4 | 1 | 1 | 436 | 1 | 435 | 0.0 | 885 |
| MS.gene51920.t1 | MTR_6g478120 | 90.115 | 435 | 42 | 1 | 1 | 434 | 1 | 435 | 0.0 | 822 |
| MS.gene51920.t1 | MTR_4g103720 | 85.315 | 429 | 62 | 1 | 1 | 428 | 1 | 429 | 0.0 | 770 |
| MS.gene51920.t1 | MTR_6g478090 | 65.714 | 245 | 62 | 1 | 190 | 434 | 4 | 226 | 5.38e-104 | 308 |
| MS.gene51920.t1 | MTR_4g103730 | 79.618 | 157 | 29 | 1 | 130 | 286 | 6 | 159 | 2.02e-84 | 256 |
| MS.gene51920.t1 | MTR_6g081000 | 62.745 | 153 | 56 | 1 | 131 | 283 | 4 | 155 | 1.27e-58 | 189 |
| MS.gene51920.t1 | MTR_7g109660 | 73.529 | 68 | 15 | 2 | 34 | 99 | 16 | 82 | 1.04e-23 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51920.t1 | AT3G26618 | 91.514 | 436 | 36 | 1 | 1 | 436 | 1 | 435 | 0.0 | 828 |
| MS.gene51920.t1 | AT1G12920 | 90.741 | 432 | 39 | 1 | 5 | 436 | 4 | 434 | 0.0 | 820 |
| MS.gene51920.t1 | AT5G47880 | 86.713 | 429 | 53 | 1 | 5 | 429 | 6 | 434 | 0.0 | 779 |
| MS.gene51920.t1 | AT5G47880 | 86.713 | 429 | 53 | 1 | 5 | 429 | 6 | 434 | 0.0 | 779 |
Find 87 sgRNAs with CRISPR-Local
Find 103 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCATTGTATGGGAGAATT+TGG | 0.171668 | 7.1:+20158557 | MS.gene51920:CDS |
| TGCAGAGGGTTTGGTGGTAT+TGG | 0.226379 | 7.1:+20158807 | MS.gene51920:CDS |
| CCTCCTCTACCATGTTTCTT+TGG | 0.261663 | 7.1:-20158123 | None:intergenic |
| GTCTCGTATGGTGGGGAAAA+TGG | 0.297905 | 7.1:+20158366 | MS.gene51920:CDS |
| GCGAATGAGTACAAAAGATT+TGG | 0.305651 | 7.1:+20158735 | MS.gene51920:CDS |
| AAGATGTTGGGAGATGAATT+TGG | 0.306796 | 7.1:+20157742 | MS.gene51920:CDS |
| TGCGGCCACATCTGCAGATT+TGG | 0.309390 | 7.1:+20158677 | MS.gene51920:CDS |
| CGACCCTTCTTGAGATTTGT+TGG | 0.312643 | 7.1:-20158778 | None:intergenic |
| CTAGAATCTGATGACAAGTT+TGG | 0.315461 | 7.1:+20158012 | MS.gene51920:CDS |
| AATCCAAATATGCACTTAAT+CGG | 0.359874 | 7.1:-20158904 | None:intergenic |
| CACATTGAAGGCTCTAGAAA+TGG | 0.360512 | 7.1:+20158521 | MS.gene51920:CDS |
| ATCTGCAGATTTGGAGGTTC+AGG | 0.366347 | 7.1:+20158686 | MS.gene51920:CDS |
| TGGCTCCAAGCACTGATTGC+CGG | 0.392694 | 7.1:-20157795 | None:intergenic |
| ATGTGGCCGCATCGCGGAAG+TGG | 0.405903 | 7.1:-20158665 | None:intergenic |
| AGAGGGTTTGGTGGTATTGG+AGG | 0.406600 | 7.1:+20158810 | MS.gene51920:CDS |
| GAGACTGGTAAATATGTTTA+CGG | 0.407711 | 7.1:+20158489 | MS.gene51920:CDS |
| AGATAAAGTGATGCATTGAT+TGG | 0.409138 | 7.1:-20157949 | None:intergenic |
| CGGTTGAAACACTCATTGTA+TGG | 0.412463 | 7.1:+20158547 | MS.gene51920:CDS |
| TTTATTGTTATGGATGGAAA+TGG | 0.413603 | 7.1:+20158036 | MS.gene51920:CDS |
| ATTCAAGAGAAACGCTTGAT+AGG | 0.417924 | 7.1:+20158441 | MS.gene51920:CDS |
| TTCCACTTAATGTCCCGAAA+AGG | 0.426411 | 7.1:-20158062 | None:intergenic |
| TCCACTTAATGTCCCGAAAA+GGG | 0.426867 | 7.1:-20158061 | None:intergenic |
| TGTAATAAAGCATTTGAAGA+AGG | 0.432701 | 7.1:+20158623 | MS.gene51920:CDS |
| CAGACAAGAATATTGAGATA+TGG | 0.434895 | 7.1:+20157617 | MS.gene51920:CDS |
| ACTAGTCAGCCTAATGTTGC+TGG | 0.435213 | 7.1:+20158246 | MS.gene51920:CDS |
| TAATGTTGCTGGACTCATAC+TGG | 0.438513 | 7.1:+20158257 | MS.gene51920:CDS |
| AAACATATTTACCAGTCTCT+TGG | 0.438527 | 7.1:-20158485 | None:intergenic |
| CTGGAAGCTGCAAGAGGTAA+TGG | 0.449382 | 7.1:+20157664 | MS.gene51920:CDS |
| ACATCAAGAGTAGGGTAAAC+CGG | 0.459780 | 7.1:+20157776 | MS.gene51920:CDS |
| GTATAAAGAACCAATCCATT+TGG | 0.460218 | 7.1:-20157862 | None:intergenic |
| AGTATGAGTCCAGCAACATT+AGG | 0.468191 | 7.1:-20158255 | None:intergenic |
| TATAACAAGGTTCCTCCAAA+TGG | 0.471094 | 7.1:+20157847 | MS.gene51920:CDS |
| ATCTCGTGTCACTAAGATGT+TGG | 0.473352 | 7.1:+20157729 | MS.gene51920:CDS |
| CGGAGTTGATGACACATTGA+AGG | 0.474084 | 7.1:+20158509 | MS.gene51920:CDS |
| CAAGGTTCCTCCAAATGGAT+TGG | 0.477410 | 7.1:+20157852 | MS.gene51920:CDS |
| CTTGCCGTCCTCAGTGAGAA+TGG | 0.478058 | 7.1:-20157890 | None:intergenic |
| AATGGATTGGTTCTTTATAC+CGG | 0.484935 | 7.1:+20157865 | MS.gene51920:CDS |
| TCTCGTGTCACTAAGATGTT+GGG | 0.490357 | 7.1:+20157730 | MS.gene51920:CDS |
| ACATTAGGCTGACTAGTAGC+AGG | 0.499450 | 7.1:-20158240 | None:intergenic |
| CAAAAGATTTGGATGCTCGC+TGG | 0.500903 | 7.1:+20158746 | MS.gene51920:CDS |
| ACATTGAAGGCTCTAGAAAT+GGG | 0.514674 | 7.1:+20158522 | MS.gene51920:CDS |
| GGGAGAATTTGGATATTACT+AGG | 0.521356 | 7.1:+20158568 | MS.gene51920:CDS |
| GTAATAAAGCATTTGAAGAA+GGG | 0.522658 | 7.1:+20158624 | MS.gene51920:CDS |
| CCTCTGTTGAGCAGAAGTAA+TGG | 0.522824 | 7.1:-20157815 | None:intergenic |
| GGCCACATCTGCAGATTTGG+AGG | 0.524825 | 7.1:+20158680 | MS.gene51920:CDS |
| GTAAACCGGCAATCAGTGCT+TGG | 0.540063 | 7.1:+20157790 | MS.gene51920:CDS |
| CTGCTTCAAACATCAAGAGT+AGG | 0.540306 | 7.1:+20157767 | MS.gene51920:CDS |
| TTCGCAAACCACTCAAGTAA+TGG | 0.547585 | 7.1:-20158717 | None:intergenic |
| GAAGAGATAAGCCAAGAGAC+TGG | 0.550060 | 7.1:+20158474 | MS.gene51920:CDS |
| TGAGGACGGCAAGGAAAAGA+AGG | 0.553356 | 7.1:+20157900 | MS.gene51920:CDS |
| GTGGTTGATGTCTCGTATGG+TGG | 0.555643 | 7.1:+20158357 | MS.gene51920:CDS |
| AACCTCCAAATCTGCAGATG+TGG | 0.562932 | 7.1:-20158682 | None:intergenic |
| AGAAGATGCCATTACTTGAG+TGG | 0.565327 | 7.1:+20158709 | MS.gene51920:CDS |
| GATGCATTGATTGGTTTGAA+AGG | 0.571596 | 7.1:-20157940 | None:intergenic |
| GTGACCAACAAATCTCAAGA+AGG | 0.573488 | 7.1:+20158774 | MS.gene51920:CDS |
| TGCTTCAAACATCAAGAGTA+GGG | 0.575365 | 7.1:+20157768 | MS.gene51920:CDS |
| TAAAGAACCAATCCATTTGG+AGG | 0.576280 | 7.1:-20157859 | None:intergenic |
| GTCCTCAGTGAGAATGGTAC+CGG | 0.581083 | 7.1:-20157884 | None:intergenic |
| CGAATGATCTGATATCAAGC+TGG | 0.590096 | 7.1:-20158845 | None:intergenic |
| GATGAGTTATCTGATGATGA+TGG | 0.590510 | 7.1:+20158867 | MS.gene51920:CDS |
| TGGTTGATGTCTCGTATGGT+GGG | 0.591547 | 7.1:+20158358 | MS.gene51920:CDS |
| CTACCAAAGAAACATGGTAG+AGG | 0.592428 | 7.1:+20158120 | MS.gene51920:CDS |
| ATTCTTGTCTGATTCACCAT+CGG | 0.594219 | 7.1:-20157605 | None:intergenic |
| GTTGCTGGACTCATACTGGC+TGG | 0.598808 | 7.1:+20158261 | MS.gene51920:CDS |
| CATTCTCACTGAGGACGGCA+AGG | 0.604123 | 7.1:+20157891 | MS.gene51920:CDS |
| GGTTGAAACACTCATTGTAT+GGG | 0.621105 | 7.1:+20158548 | MS.gene51920:CDS |
| AACGTGGTTGATGTCTCGTA+TGG | 0.621452 | 7.1:+20158354 | MS.gene51920:CDS |
| CCAAAGAAACATGGTAGAGG+AGG | 0.625338 | 7.1:+20158123 | MS.gene51920:CDS |
| TGACCAACAAATCTCAAGAA+GGG | 0.625562 | 7.1:+20158775 | MS.gene51920:CDS |
| CCATTACTTCTGCTCAACAG+AGG | 0.649740 | 7.1:+20157815 | MS.gene51920:CDS |
| GGTACCATTCTCACTGAGGA+CGG | 0.651299 | 7.1:+20157886 | MS.gene51920:CDS |
| GAAGGCTCTAGAAATGGGTG+CGG | 0.653960 | 7.1:+20158527 | MS.gene51920:CDS |
| ACACGAGATATTTGATCACG+TGG | 0.654304 | 7.1:-20157715 | None:intergenic |
| CATTAGGCTGACTAGTAGCA+GGG | 0.656179 | 7.1:-20158239 | None:intergenic |
| AAAGGACTGGAAGCTGCAAG+AGG | 0.664338 | 7.1:+20157658 | MS.gene51920:CDS |
| CTTTATTACAATCTCACCAG+TGG | 0.665088 | 7.1:-20158610 | None:intergenic |
| TCAAAGCCACTTCCGCGATG+CGG | 0.668435 | 7.1:+20158659 | MS.gene51920:CDS |
| GAGGTTGAAACTATATAACA+AGG | 0.687653 | 7.1:+20157834 | MS.gene51920:CDS |
| AGGGTTGATGTAAAACTGAG+TGG | 0.688267 | 7.1:-20158220 | None:intergenic |
| CTGCAGATGTGGCCGCATCG+CGG | 0.688811 | 7.1:-20158671 | None:intergenic |
| TCTGCATAAACAATGGCCGA+TGG | 0.701549 | 7.1:+20157589 | None:intergenic |
| GGTTGATGTCTCGTATGGTG+GGG | 0.707328 | 7.1:+20158359 | MS.gene51920:CDS |
| CGAGATATTTGATCACGTGG+AGG | 0.714871 | 7.1:-20157712 | None:intergenic |
| GTTGATCTACCAAAGAAACA+TGG | 0.720655 | 7.1:+20158114 | MS.gene51920:CDS |
| ATTAAGTGGAAATACTCGCG+AGG | 0.721816 | 7.1:+20158074 | MS.gene51920:CDS |
| TGATGATGATGGAGTGTACG+AGG | 0.728062 | 7.1:+20158878 | MS.gene51920:CDS |
| TACCGGTACCATTCTCACTG+AGG | 0.737721 | 7.1:+20157882 | MS.gene51920:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTATTGTTATGGATGGAAA+TGG | + | chr7.1:20158036-20158055 | MS.gene51920:CDS | 25.0% |
| !! | AAGATCAAAAAGTTGATCAA+AGG | + | chr7.1:20157640-20157659 | MS.gene51920:CDS | 25.0% |
| !! | GTAATAAAGCATTTGAAGAA+GGG | + | chr7.1:20158624-20158643 | MS.gene51920:CDS | 25.0% |
| !! | TGTAATAAAGCATTTGAAGA+AGG | + | chr7.1:20158623-20158642 | MS.gene51920:CDS | 25.0% |
| !!! | CAAGTTTGGTTTTATTGTTA+TGG | + | chr7.1:20158026-20158045 | MS.gene51920:CDS | 25.0% |
| !!! | TTTGGTTTTATTGTTATGGA+TGG | + | chr7.1:20158030-20158049 | MS.gene51920:CDS | 25.0% |
| AAACATATTTACCAGTCTCT+TGG | - | chr7.1:20158488-20158507 | None:intergenic | 30.0% | |
| CAGACAAGAATATTGAGATA+TGG | + | chr7.1:20157617-20157636 | MS.gene51920:CDS | 30.0% | |
| GAGACTGGTAAATATGTTTA+CGG | + | chr7.1:20158489-20158508 | MS.gene51920:CDS | 30.0% | |
| GAGGTTGAAACTATATAACA+AGG | + | chr7.1:20157834-20157853 | MS.gene51920:CDS | 30.0% | |
| GTATAAAGAACCAATCCATT+TGG | - | chr7.1:20157865-20157884 | None:intergenic | 30.0% | |
| ! | AGATAAAGTGATGCATTGAT+TGG | - | chr7.1:20157952-20157971 | None:intergenic | 30.0% |
| !! | AATATTTTTGCTTGCAAACG+AGG | - | chr7.1:20158333-20158352 | None:intergenic | 30.0% |
| !! | AATGGATTGGTTCTTTATAC+CGG | + | chr7.1:20157865-20157884 | MS.gene51920:CDS | 30.0% |
| AAAAACGACACAATTACGTC+AGG | + | chr7.1:20158181-20158200 | MS.gene51920:CDS | 35.0% | |
| AAGATGTTGGGAGATGAATT+TGG | + | chr7.1:20157742-20157761 | MS.gene51920:CDS | 35.0% | |
| ACTCATTGTATGGGAGAATT+TGG | + | chr7.1:20158557-20158576 | MS.gene51920:CDS | 35.0% | |
| ATTCAAGAGAAACGCTTGAT+AGG | + | chr7.1:20158441-20158460 | MS.gene51920:CDS | 35.0% | |
| ATTCTTGTCTGATTCACCAT+CGG | - | chr7.1:20157608-20157627 | None:intergenic | 35.0% | |
| CTAGAATCTGATGACAAGTT+TGG | + | chr7.1:20158012-20158031 | MS.gene51920:CDS | 35.0% | |
| CTTTATTACAATCTCACCAG+TGG | - | chr7.1:20158613-20158632 | None:intergenic | 35.0% | |
| GCAAGCAAAAATATTGAACG+TGG | + | chr7.1:20158338-20158357 | MS.gene51920:CDS | 35.0% | |
| GCGAATGAGTACAAAAGATT+TGG | + | chr7.1:20158735-20158754 | MS.gene51920:CDS | 35.0% | |
| GGGAGAATTTGGATATTACT+AGG | + | chr7.1:20158568-20158587 | MS.gene51920:CDS | 35.0% | |
| GGTTGAAACACTCATTGTAT+GGG | + | chr7.1:20158548-20158567 | MS.gene51920:CDS | 35.0% | |
| GTTGATCTACCAAAGAAACA+TGG | + | chr7.1:20158114-20158133 | MS.gene51920:CDS | 35.0% | |
| TAAAGAACCAATCCATTTGG+AGG | - | chr7.1:20157862-20157881 | None:intergenic | 35.0% | |
| TATAACAAGGTTCCTCCAAA+TGG | + | chr7.1:20157847-20157866 | MS.gene51920:CDS | 35.0% | |
| TGACCAACAAATCTCAAGAA+GGG | + | chr7.1:20158775-20158794 | MS.gene51920:CDS | 35.0% | |
| TGCTTCAAACATCAAGAGTA+GGG | + | chr7.1:20157768-20157787 | MS.gene51920:CDS | 35.0% | |
| ! | CAAAAAGTTGATCAAAGGAC+TGG | + | chr7.1:20157645-20157664 | MS.gene51920:CDS | 35.0% |
| ! | GATGAGTTATCTGATGATGA+TGG | + | chr7.1:20158867-20158886 | MS.gene51920:CDS | 35.0% |
| !! | ACATTGAAGGCTCTAGAAAT+GGG | + | chr7.1:20158522-20158541 | MS.gene51920:CDS | 35.0% |
| !! | GATGCATTGATTGGTTTGAA+AGG | - | chr7.1:20157943-20157962 | None:intergenic | 35.0% |
| !!! | AATTGTGTCGTTTTTCCATC+CGG | - | chr7.1:20158176-20158195 | None:intergenic | 35.0% |
| ACACGAGATATTTGATCACG+TGG | - | chr7.1:20157718-20157737 | None:intergenic | 40.0% | |
| ACATCAAGAGTAGGGTAAAC+CGG | + | chr7.1:20157776-20157795 | MS.gene51920:CDS | 40.0% | |
| AGAAGATGCCATTACTTGAG+TGG | + | chr7.1:20158709-20158728 | MS.gene51920:CDS | 40.0% | |
| AGTATGAGTCCAGCAACATT+AGG | - | chr7.1:20158258-20158277 | None:intergenic | 40.0% | |
| ATTAAGTGGAAATACTCGCG+AGG | + | chr7.1:20158074-20158093 | MS.gene51920:CDS | 40.0% | |
| CGAATGATCTGATATCAAGC+TGG | - | chr7.1:20158848-20158867 | None:intergenic | 40.0% | |
| CGGTTGAAACACTCATTGTA+TGG | + | chr7.1:20158547-20158566 | MS.gene51920:CDS | 40.0% | |
| CTACCAAAGAAACATGGTAG+AGG | + | chr7.1:20158120-20158139 | MS.gene51920:CDS | 40.0% | |
| CTGCTTCAAACATCAAGAGT+AGG | + | chr7.1:20157767-20157786 | MS.gene51920:CDS | 40.0% | |
| GTGACCAACAAATCTCAAGA+AGG | + | chr7.1:20158774-20158793 | MS.gene51920:CDS | 40.0% | |
| TAATGTTGCTGGACTCATAC+TGG | + | chr7.1:20158257-20158276 | MS.gene51920:CDS | 40.0% | |
| TCCACTTAATGTCCCGAAAA+GGG | - | chr7.1:20158064-20158083 | None:intergenic | 40.0% | |
| TTCCACTTAATGTCCCGAAA+AGG | - | chr7.1:20158065-20158084 | None:intergenic | 40.0% | |
| TTCGCAAACCACTCAAGTAA+TGG | - | chr7.1:20158720-20158739 | None:intergenic | 40.0% | |
| ! | ATCTCGTGTCACTAAGATGT+TGG | + | chr7.1:20157729-20157748 | MS.gene51920:CDS | 40.0% |
| ! | ATGGAAATGGAACCCTTTTC+GGG | + | chr7.1:20158049-20158068 | MS.gene51920:CDS | 40.0% |
| ! | GATGGAAATGGAACCCTTTT+CGG | + | chr7.1:20158048-20158067 | MS.gene51920:CDS | 40.0% |
| ! | TCTCGTGTCACTAAGATGTT+GGG | + | chr7.1:20157730-20157749 | MS.gene51920:CDS | 40.0% |
| !! | AGGGTTGATGTAAAACTGAG+TGG | - | chr7.1:20158223-20158242 | None:intergenic | 40.0% |
| !! | CACATTGAAGGCTCTAGAAA+TGG | + | chr7.1:20158521-20158540 | MS.gene51920:CDS | 40.0% |
| AACCTCCAAATCTGCAGATG+TGG | - | chr7.1:20158685-20158704 | None:intergenic | 45.0% | |
| ACATTAGGCTGACTAGTAGC+AGG | - | chr7.1:20158243-20158262 | None:intergenic | 45.0% | |
| ACTAGTCAGCCTAATGTTGC+TGG | + | chr7.1:20158246-20158265 | MS.gene51920:CDS | 45.0% | |
| ATCTGCAGATTTGGAGGTTC+AGG | + | chr7.1:20158686-20158705 | MS.gene51920:CDS | 45.0% | |
| CAAAAGATTTGGATGCTCGC+TGG | + | chr7.1:20158746-20158765 | MS.gene51920:CDS | 45.0% | |
| CAAGGTTCCTCCAAATGGAT+TGG | + | chr7.1:20157852-20157871 | MS.gene51920:CDS | 45.0% | |
| CATTAGGCTGACTAGTAGCA+GGG | - | chr7.1:20158242-20158261 | None:intergenic | 45.0% | |
| CCAAAGAAACATGGTAGAGG+AGG | + | chr7.1:20158123-20158142 | MS.gene51920:CDS | 45.0% | |
| CCATTACTTCTGCTCAACAG+AGG | + | chr7.1:20157815-20157834 | MS.gene51920:CDS | 45.0% | |
| CCTCCTCTACCATGTTTCTT+TGG | - | chr7.1:20158126-20158145 | None:intergenic | 45.0% | |
| CCTCTGTTGAGCAGAAGTAA+TGG | - | chr7.1:20157818-20157837 | None:intergenic | 45.0% | |
| CGACCCTTCTTGAGATTTGT+TGG | - | chr7.1:20158781-20158800 | None:intergenic | 45.0% | |
| CGAGATATTTGATCACGTGG+AGG | - | chr7.1:20157715-20157734 | None:intergenic | 45.0% | |
| GAAGAGATAAGCCAAGAGAC+TGG | + | chr7.1:20158474-20158493 | MS.gene51920:CDS | 45.0% | |
| GTGCTGAAAAATGCTACCAC+TGG | + | chr7.1:20158594-20158613 | MS.gene51920:CDS | 45.0% | |
| ! | AACGTGGTTGATGTCTCGTA+TGG | + | chr7.1:20158354-20158373 | MS.gene51920:CDS | 45.0% |
| ! | TGGTTGATGTCTCGTATGGT+GGG | + | chr7.1:20158358-20158377 | MS.gene51920:CDS | 45.0% |
| !! | ACCCTTTTCGGGACATTAAG+TGG | + | chr7.1:20158060-20158079 | MS.gene51920:CDS | 45.0% |
| !! | CGGAGTTGATGACACATTGA+AGG | + | chr7.1:20158509-20158528 | MS.gene51920:CDS | 45.0% |
| !! | TGATGATGATGGAGTGTACG+AGG | + | chr7.1:20158878-20158897 | MS.gene51920:CDS | 45.0% |
| AAAGGACTGGAAGCTGCAAG+AGG | + | chr7.1:20157658-20157677 | MS.gene51920:CDS | 50.0% | |
| CTGGAAGCTGCAAGAGGTAA+TGG | + | chr7.1:20157664-20157683 | MS.gene51920:CDS | 50.0% | |
| GGTACCATTCTCACTGAGGA+CGG | + | chr7.1:20157886-20157905 | MS.gene51920:CDS | 50.0% | |
| GTAAACCGGCAATCAGTGCT+TGG | + | chr7.1:20157790-20157809 | MS.gene51920:CDS | 50.0% | |
| GTCCTCAGTGAGAATGGTAC+CGG | - | chr7.1:20157887-20157906 | None:intergenic | 50.0% | |
| TACCGGTACCATTCTCACTG+AGG | + | chr7.1:20157882-20157901 | MS.gene51920:CDS | 50.0% | |
| TGAGGACGGCAAGGAAAAGA+AGG | + | chr7.1:20157900-20157919 | MS.gene51920:CDS | 50.0% | |
| ! | AAGGGTCGCAGTTTTGCAGA+GGG | + | chr7.1:20158793-20158812 | MS.gene51920:CDS | 50.0% |
| ! | CAGTTTTGCAGAGGGTTTGG+TGG | + | chr7.1:20158801-20158820 | MS.gene51920:CDS | 50.0% |
| ! | CTTTGCGTTTTGCTCGTCTC+CGG | + | chr7.1:20158154-20158173 | MS.gene51920:CDS | 50.0% |
| ! | GGTTGATGTCTCGTATGGTG+GGG | + | chr7.1:20158359-20158378 | MS.gene51920:CDS | 50.0% |
| ! | GTCTCGTATGGTGGGGAAAA+TGG | + | chr7.1:20158366-20158385 | MS.gene51920:CDS | 50.0% |
| ! | GTGGTTGATGTCTCGTATGG+TGG | + | chr7.1:20158357-20158376 | MS.gene51920:CDS | 50.0% |
| ! | TCGCAGTTTTGCAGAGGGTT+TGG | + | chr7.1:20158798-20158817 | MS.gene51920:CDS | 50.0% |
| !! | AGAGGGTTTGGTGGTATTGG+AGG | + | chr7.1:20158810-20158829 | MS.gene51920:CDS | 50.0% |
| !! | GAAGGCTCTAGAAATGGGTG+CGG | + | chr7.1:20158527-20158546 | MS.gene51920:CDS | 50.0% |
| !! | TGCAGAGGGTTTGGTGGTAT+TGG | + | chr7.1:20158807-20158826 | MS.gene51920:CDS | 50.0% |
| CATTCTCACTGAGGACGGCA+AGG | + | chr7.1:20157891-20157910 | MS.gene51920:CDS | 55.0% | |
| CTTGCCGTCCTCAGTGAGAA+TGG | - | chr7.1:20157893-20157912 | None:intergenic | 55.0% | |
| GGCCACATCTGCAGATTTGG+AGG | + | chr7.1:20158680-20158699 | MS.gene51920:CDS | 55.0% | |
| GTTGCTGGACTCATACTGGC+TGG | + | chr7.1:20158261-20158280 | MS.gene51920:CDS | 55.0% | |
| TCAAAGCCACTTCCGCGATG+CGG | + | chr7.1:20158659-20158678 | MS.gene51920:CDS | 55.0% | |
| TGCGGCCACATCTGCAGATT+TGG | + | chr7.1:20158677-20158696 | MS.gene51920:CDS | 55.0% | |
| ! | GAAGGGTCGCAGTTTTGCAG+AGG | + | chr7.1:20158792-20158811 | MS.gene51920:CDS | 55.0% |
| !! | TGGCTCCAAGCACTGATTGC+CGG | - | chr7.1:20157798-20157817 | None:intergenic | 55.0% |
| ! | GCGTTTTGCTCGTCTCCGGA+TGG | + | chr7.1:20158158-20158177 | MS.gene51920:CDS | 60.0% |
| ATGTGGCCGCATCGCGGAAG+TGG | - | chr7.1:20158668-20158687 | None:intergenic | 65.0% | |
| CTGCAGATGTGGCCGCATCG+CGG | - | chr7.1:20158674-20158693 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 20157601 | 20158911 | 20157601 | ID=MS.gene51920 |
| chr7.1 | mRNA | 20157601 | 20158911 | 20157601 | ID=MS.gene51920.t1;Parent=MS.gene51920 |
| chr7.1 | exon | 20157601 | 20158911 | 20157601 | ID=MS.gene51920.t1.exon1;Parent=MS.gene51920.t1 |
| chr7.1 | CDS | 20157601 | 20158911 | 20157601 | ID=cds.MS.gene51920.t1;Parent=MS.gene51920.t1 |
| Gene Sequence |
| Protein sequence |
