Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20064.t1 | XP_003625632.1 | 93.4 | 166 | 5 | 2 | 1 | 166 | 1 | 160 | 1.00E-70 | 276.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20064.t1 | Q2HV51 | 93.4 | 166 | 5 | 2 | 1 | 166 | 1 | 160 | 7.3e-71 | 276.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene20064 | 0.804361 | 2.24E-49 | -1.69E-46 |
| MS.gene04919 | MS.gene20064 | 0.805742 | 1.15E-49 | -1.69E-46 |
| MS.gene04920 | MS.gene20064 | 0.818457 | 1.94E-52 | -1.69E-46 |
| MS.gene049283 | MS.gene20064 | 0.846046 | 2.78E-59 | -1.69E-46 |
| MS.gene049499 | MS.gene20064 | 0.812633 | 3.84E-51 | -1.69E-46 |
| MS.gene049983 | MS.gene20064 | 0.821767 | 3.39E-53 | -1.69E-46 |
| MS.gene04998 | MS.gene20064 | 0.812252 | 4.65E-51 | -1.69E-46 |
| MS.gene050359 | MS.gene20064 | 0.802755 | 4.83E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene20064 | 0.826351 | 2.85E-54 | -1.69E-46 |
| MS.gene050646 | MS.gene20064 | 0.837999 | 3.73E-57 | -1.69E-46 |
| MS.gene050752 | MS.gene20064 | 0.834438 | 3.00E-56 | -1.69E-46 |
| MS.gene050838 | MS.gene20064 | 0.80596 | 1.04E-49 | -1.69E-46 |
| MS.gene050945 | MS.gene20064 | 0.857494 | 1.57E-62 | -1.69E-46 |
| MS.gene050955 | MS.gene20064 | 0.833869 | 4.16E-56 | -1.69E-46 |
| MS.gene051003 | MS.gene20064 | 0.812193 | 4.79E-51 | -1.69E-46 |
| MS.gene051043 | MS.gene20064 | 0.828135 | 1.06E-54 | -1.69E-46 |
| MS.gene051226 | MS.gene20064 | 0.804392 | 2.21E-49 | -1.69E-46 |
| MS.gene051234 | MS.gene20064 | 0.819535 | 1.10E-52 | -1.69E-46 |
| MS.gene051343 | MS.gene20064 | 0.840395 | 8.92E-58 | -1.69E-46 |
| MS.gene051676 | MS.gene20064 | 0.804464 | 2.13E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene20064 | 0.823323 | 1.47E-53 | -1.69E-46 |
| MS.gene051856 | MS.gene20064 | 0.809725 | 1.64E-50 | -1.69E-46 |
| MS.gene051902 | MS.gene20064 | 0.847216 | 1.33E-59 | -1.69E-46 |
| MS.gene051975 | MS.gene20064 | 0.806051 | 9.92E-50 | -1.69E-46 |
| MS.gene052016 | MS.gene20064 | 0.803606 | 3.22E-49 | -1.69E-46 |
| MS.gene052072 | MS.gene20064 | 0.821701 | 3.51E-53 | -1.69E-46 |
| MS.gene052239 | MS.gene20064 | -0.80879 | 2.60E-50 | -1.69E-46 |
| MS.gene052242 | MS.gene20064 | -0.827965 | 1.17E-54 | -1.69E-46 |
| MS.gene052296 | MS.gene20064 | 0.830072 | 3.61E-55 | -1.69E-46 |
| MS.gene052479 | MS.gene20064 | 0.803688 | 3.09E-49 | -1.69E-46 |
| MS.gene052533 | MS.gene20064 | 0.803917 | 2.77E-49 | -1.69E-46 |
| MS.gene052793 | MS.gene20064 | 0.836073 | 1.16E-56 | -1.69E-46 |
| MS.gene053045 | MS.gene20064 | 0.826383 | 2.80E-54 | -1.69E-46 |
| MS.gene053189 | MS.gene20064 | 0.843801 | 1.12E-58 | -1.69E-46 |
| MS.gene053298 | MS.gene20064 | 0.808059 | 3.73E-50 | -1.69E-46 |
| MS.gene053564 | MS.gene20064 | 0.826997 | 2.00E-54 | -1.69E-46 |
| MS.gene053879 | MS.gene20064 | 0.804979 | 1.67E-49 | -1.69E-46 |
| MS.gene053931 | MS.gene20064 | 0.819289 | 1.26E-52 | -1.69E-46 |
| MS.gene053963 | MS.gene20064 | 0.816541 | 5.24E-52 | -1.69E-46 |
| MS.gene054004 | MS.gene20064 | 0.805755 | 1.15E-49 | -1.69E-46 |
| MS.gene054222 | MS.gene20064 | 0.815112 | 1.09E-51 | -1.69E-46 |
| MS.gene054403 | MS.gene20064 | 0.839224 | 1.80E-57 | -1.69E-46 |
| MS.gene054413 | MS.gene20064 | 0.813116 | 3.01E-51 | -1.69E-46 |
| MS.gene054536 | MS.gene20064 | 0.801739 | 7.81E-49 | -1.69E-46 |
| MS.gene05469 | MS.gene20064 | 0.80327 | 3.78E-49 | -1.69E-46 |
| MS.gene054709 | MS.gene20064 | 0.8038 | 2.93E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene20064 | 0.811232 | 7.76E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene20064 | 0.815605 | 8.48E-52 | -1.69E-46 |
| MS.gene055841 | MS.gene20064 | 0.808013 | 3.82E-50 | -1.69E-46 |
| MS.gene056082 | MS.gene20064 | 0.844452 | 7.50E-59 | -1.69E-46 |
| MS.gene057102 | MS.gene20064 | 0.84277 | 2.11E-58 | -1.69E-46 |
| MS.gene057208 | MS.gene20064 | 0.813834 | 2.09E-51 | -1.69E-46 |
| MS.gene057622 | MS.gene20064 | 0.815574 | 8.62E-52 | -1.69E-46 |
| MS.gene057694 | MS.gene20064 | 0.812804 | 3.53E-51 | -1.69E-46 |
| MS.gene057705 | MS.gene20064 | 0.826906 | 2.10E-54 | -1.69E-46 |
| MS.gene057890 | MS.gene20064 | -0.82407 | 9.85E-54 | -1.69E-46 |
| MS.gene057999 | MS.gene20064 | 0.82605 | 3.36E-54 | -1.69E-46 |
| MS.gene058008 | MS.gene20064 | 0.809195 | 2.13E-50 | -1.69E-46 |
| MS.gene058074 | MS.gene20064 | 0.82398 | 1.03E-53 | -1.69E-46 |
| MS.gene058159 | MS.gene20064 | 0.807901 | 4.03E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene20064 | 0.813039 | 3.13E-51 | -1.69E-46 |
| MS.gene058279 | MS.gene20064 | 0.812385 | 4.36E-51 | -1.69E-46 |
| MS.gene058592 | MS.gene20064 | 0.818362 | 2.04E-52 | -1.69E-46 |
| MS.gene059146 | MS.gene20064 | 0.818493 | 1.90E-52 | -1.69E-46 |
| MS.gene059170 | MS.gene20064 | 0.808792 | 2.60E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene20064 | 0.822598 | 2.17E-53 | -1.69E-46 |
| MS.gene05985 | MS.gene20064 | -0.84108 | 5.90E-58 | -1.69E-46 |
| MS.gene06018 | MS.gene20064 | 0.815593 | 8.54E-52 | -1.69E-46 |
| MS.gene060244 | MS.gene20064 | 0.849173 | 3.83E-60 | -1.69E-46 |
| MS.gene060259 | MS.gene20064 | 0.821745 | 3.43E-53 | -1.69E-46 |
| MS.gene060365 | MS.gene20064 | 0.825787 | 3.87E-54 | -1.69E-46 |
| MS.gene060933 | MS.gene20064 | 0.804388 | 2.21E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene20064 | 0.806976 | 6.33E-50 | -1.69E-46 |
| MS.gene060937 | MS.gene20064 | 0.817392 | 3.38E-52 | -1.69E-46 |
| MS.gene061100 | MS.gene20064 | 0.834738 | 2.52E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20064.t1 | MTR_7g101250 | 94.578 | 166 | 3 | 2 | 1 | 166 | 1 | 160 | 4.30e-111 | 313 |
| MS.gene20064.t1 | MTR_2g094410 | 43.312 | 157 | 73 | 5 | 11 | 166 | 15 | 156 | 4.52e-31 | 110 |
| MS.gene20064.t1 | MTR_4g059320 | 47.368 | 133 | 62 | 4 | 36 | 165 | 31 | 158 | 2.20e-30 | 108 |
| MS.gene20064.t1 | MTR_4g132020 | 35.450 | 189 | 84 | 8 | 1 | 165 | 1 | 175 | 1.19e-21 | 86.7 |
| MS.gene20064.t1 | MTR_2g023070 | 47.059 | 85 | 40 | 2 | 82 | 165 | 137 | 217 | 1.97e-17 | 76.3 |
| MS.gene20064.t1 | MTR_2g023390 | 51.163 | 86 | 34 | 2 | 83 | 164 | 73 | 154 | 5.83e-16 | 71.2 |
| MS.gene20064.t1 | MTR_2g086660 | 40.230 | 87 | 47 | 2 | 84 | 166 | 170 | 255 | 4.96e-12 | 62.4 |
| MS.gene20064.t1 | MTR_3g052330 | 39.091 | 110 | 38 | 4 | 84 | 164 | 87 | 196 | 1.41e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20064.t1 | AT5G03230 | 40.805 | 174 | 84 | 5 | 3 | 166 | 2 | 166 | 3.21e-32 | 113 |
| MS.gene20064.t1 | AT3G45210 | 43.636 | 165 | 74 | 4 | 2 | 166 | 3 | 148 | 1.20e-29 | 106 |
| MS.gene20064.t1 | AT2G28400 | 38.824 | 170 | 92 | 3 | 1 | 166 | 1 | 162 | 9.39e-28 | 102 |
| MS.gene20064.t1 | AT5G60680 | 43.023 | 172 | 74 | 5 | 6 | 166 | 5 | 163 | 1.50e-27 | 101 |
| MS.gene20064.t1 | AT4G21970 | 49.462 | 93 | 28 | 4 | 83 | 164 | 59 | 143 | 2.14e-17 | 75.1 |
| MS.gene20064.t1 | AT4G04630 | 38.298 | 141 | 74 | 4 | 37 | 164 | 26 | 166 | 7.36e-17 | 73.9 |
| MS.gene20064.t1 | AT1G11700 | 43.750 | 96 | 44 | 2 | 76 | 164 | 107 | 199 | 1.42e-15 | 71.2 |
| MS.gene20064.t1 | AT1G61930 | 46.154 | 91 | 39 | 2 | 82 | 164 | 113 | 201 | 4.93e-15 | 69.7 |
| MS.gene20064.t1 | AT4G26950 | 36.170 | 141 | 64 | 5 | 37 | 164 | 15 | 142 | 3.28e-14 | 66.2 |
| MS.gene20064.t1 | AT4G26950 | 36.170 | 141 | 64 | 5 | 37 | 164 | 15 | 142 | 3.28e-14 | 66.2 |
| MS.gene20064.t1 | AT4G21930 | 35.897 | 156 | 66 | 8 | 35 | 164 | 34 | 181 | 9.15e-14 | 66.2 |
| MS.gene20064.t1 | AT3G15040 | 41.379 | 87 | 47 | 1 | 84 | 166 | 157 | 243 | 2.29e-12 | 63.2 |
| MS.gene20064.t1 | AT1G29640 | 41.284 | 109 | 43 | 5 | 65 | 164 | 41 | 137 | 1.11e-11 | 59.7 |
Find 29 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCAATCAATGGCTTCTTCT+AGG | 0.289420 | 7.2:-11085365 | None:intergenic |
| GTATTCATCCTTCAAAATCT+TGG | 0.290307 | 7.2:+11085080 | None:intergenic |
| GGGAAAGGAAGGACTTTAAA+AGG | 0.299267 | 7.2:-11084937 | MS.gene20064:CDS |
| GTAGTGTAAGGAATGCTATT+TGG | 0.358519 | 7.2:-11084903 | MS.gene20064:CDS |
| ATGCTATTTGGAAGAAAGTT+GGG | 0.379786 | 7.2:-11084891 | MS.gene20064:CDS |
| GACCAATCTGGTATATTCAT+TGG | 0.380128 | 7.2:+11085102 | None:intergenic |
| AATGCTATTTGGAAGAAAGT+TGG | 0.385752 | 7.2:-11084892 | MS.gene20064:CDS |
| TTCAAAATCTTGGACCAATC+TGG | 0.413413 | 7.2:+11085090 | None:intergenic |
| CTAGAAACTCTCTTCAAACC+TGG | 0.465204 | 7.2:+11085174 | None:intergenic |
| GTTTCTGTCTCTGAGGTAGT+TGG | 0.466042 | 7.2:+11085309 | None:intergenic |
| AGTTTGATGAAGCTGATGTA+TGG | 0.474055 | 7.2:-11085248 | MS.gene20064:CDS |
| GAAGAGACTTGCGTAGTGTA+AGG | 0.480322 | 7.2:-11084915 | MS.gene20064:CDS |
| AAATGTAACTTGTTCTTGAA+AGG | 0.483499 | 7.2:+11085334 | None:intergenic |
| CTTTCTGTTCATGAAGGGAA+AGG | 0.500406 | 7.2:-11084952 | MS.gene20064:CDS |
| GAAGATGAAGGTGATTATGA+TGG | 0.512537 | 7.2:-11085030 | MS.gene20064:CDS |
| GCTTCTCTTTCTGTTCATGA+AGG | 0.513560 | 7.2:-11084958 | MS.gene20064:CDS |
| CTTCTCTTTCTGTTCATGAA+GGG | 0.517982 | 7.2:-11084957 | MS.gene20064:CDS |
| TACCAATGAATATACCAGAT+TGG | 0.518610 | 7.2:-11085104 | MS.gene20064:CDS |
| AGTAGTGATGATGAAGATGA+AGG | 0.546106 | 7.2:-11085042 | MS.gene20064:CDS |
| GGTAATGAAGATGAAGCTAA+AGG | 0.550449 | 7.2:+11085123 | None:intergenic |
| CTGTTCATGAAGGGAAAGGA+AGG | 0.560984 | 7.2:-11084948 | MS.gene20064:CDS |
| GAATATCTTCTTAGAACTAG+AGG | 0.587771 | 7.2:-11084982 | MS.gene20064:CDS |
| TGAAGGTGATTATGATGGAG+GGG | 0.591476 | 7.2:-11085025 | MS.gene20064:CDS |
| GAAATTTGTTTCTGTCTCTG+AGG | 0.611033 | 7.2:+11085302 | None:intergenic |
| GATGAAGGTGATTATGATGG+AGG | 0.617604 | 7.2:-11085027 | MS.gene20064:CDS |
| ATGAAGGTGATTATGATGGA+GGG | 0.625231 | 7.2:-11085026 | MS.gene20064:CDS |
| CTAAGAAGATATTCATGAGG+AGG | 0.629545 | 7.2:+11084991 | None:intergenic |
| AGGTGATTATGATGGAGGGG+TGG | 0.659515 | 7.2:-11085022 | MS.gene20064:CDS |
| GTTCTAAGAAGATATTCATG+AGG | 0.678087 | 7.2:+11084988 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCAATCAAAAATCATCACA+AGG | - | chr7.2:11084954-11084973 | MS.gene20064:CDS | 25.0% |
| !!! | AAATGTAACTTGTTCTTGAA+AGG | + | chr7.2:11084905-11084924 | None:intergenic | 25.0% |
| ACAAACATAGAGCCAAAAAA+GGG | - | chr7.2:11085035-11085054 | MS.gene20064:CDS | 30.0% | |
| GAATATCTTCTTAGAACTAG+AGG | - | chr7.2:11085254-11085273 | MS.gene20064:CDS | 30.0% | |
| GTATTCATCCTTCAAAATCT+TGG | + | chr7.2:11085159-11085178 | None:intergenic | 30.0% | |
| TACCAATGAATATACCAGAT+TGG | - | chr7.2:11085132-11085151 | MS.gene20064:CDS | 30.0% | |
| ! | AATGCTATTTGGAAGAAAGT+TGG | - | chr7.2:11085344-11085363 | MS.gene20064:CDS | 30.0% |
| ! | ATGCTATTTGGAAGAAAGTT+GGG | - | chr7.2:11085345-11085364 | MS.gene20064:CDS | 30.0% |
| ! | GAAGAGAGTTTCTAGAAAAA+TGG | - | chr7.2:11085070-11085089 | MS.gene20064:CDS | 30.0% |
| !! | GTTCTAAGAAGATATTCATG+AGG | + | chr7.2:11085251-11085270 | None:intergenic | 30.0% |
| AGTTTGATGAAGCTGATGTA+TGG | - | chr7.2:11084988-11085007 | MS.gene20064:CDS | 35.0% | |
| CACAAACATAGAGCCAAAAA+AGG | - | chr7.2:11085034-11085053 | MS.gene20064:CDS | 35.0% | |
| CTAAGAAGATATTCATGAGG+AGG | + | chr7.2:11085248-11085267 | None:intergenic | 35.0% | |
| CTTCTCTTTCTGTTCATGAA+GGG | - | chr7.2:11085279-11085298 | MS.gene20064:CDS | 35.0% | |
| GAAGATGAAGGTGATTATGA+TGG | - | chr7.2:11085206-11085225 | MS.gene20064:CDS | 35.0% | |
| GACCAATCTGGTATATTCAT+TGG | + | chr7.2:11085137-11085156 | None:intergenic | 35.0% | |
| GGTAATGAAGATGAAGCTAA+AGG | + | chr7.2:11085116-11085135 | None:intergenic | 35.0% | |
| GTAGTGTAAGGAATGCTATT+TGG | - | chr7.2:11085333-11085352 | MS.gene20064:CDS | 35.0% | |
| TTCAAAATCTTGGACCAATC+TGG | + | chr7.2:11085149-11085168 | None:intergenic | 35.0% | |
| ! | AGTAGTGATGATGAAGATGA+AGG | - | chr7.2:11085194-11085213 | MS.gene20064:CDS | 35.0% |
| ! | ATGAAGGTGATTATGATGGA+GGG | - | chr7.2:11085210-11085229 | MS.gene20064:CDS | 35.0% |
| !! | AGATTGGTCCAAGATTTTGA+AGG | - | chr7.2:11085148-11085167 | MS.gene20064:CDS | 35.0% |
| !! | GAAATTTGTTTCTGTCTCTG+AGG | + | chr7.2:11084937-11084956 | None:intergenic | 35.0% |
| !!! | TTTTGGCTCTATGTTTGTGT+TGG | + | chr7.2:11085033-11085052 | None:intergenic | 35.0% |
| CTAGAAACTCTCTTCAAACC+TGG | + | chr7.2:11085065-11085084 | None:intergenic | 40.0% | |
| CTTTCTGTTCATGAAGGGAA+AGG | - | chr7.2:11085284-11085303 | MS.gene20064:CDS | 40.0% | |
| GATGAAGGTGATTATGATGG+AGG | - | chr7.2:11085209-11085228 | MS.gene20064:CDS | 40.0% | |
| GCTTCTCTTTCTGTTCATGA+AGG | - | chr7.2:11085278-11085297 | MS.gene20064:CDS | 40.0% | |
| GGGAAAGGAAGGACTTTAAA+AGG | - | chr7.2:11085299-11085318 | MS.gene20064:CDS | 40.0% | |
| ! | TGAAGGTGATTATGATGGAG+GGG | - | chr7.2:11085211-11085230 | MS.gene20064:CDS | 40.0% |
| !!! | AAACCTGGTACACCCTTTTT+TGG | + | chr7.2:11085050-11085069 | None:intergenic | 40.0% |
| CTGTTCATGAAGGGAAAGGA+AGG | - | chr7.2:11085288-11085307 | MS.gene20064:CDS | 45.0% | |
| GAAGAGACTTGCGTAGTGTA+AGG | - | chr7.2:11085321-11085340 | MS.gene20064:CDS | 45.0% | |
| !! | GTTTCTGTCTCTGAGGTAGT+TGG | + | chr7.2:11084930-11084949 | None:intergenic | 45.0% |
| ! | AGGTGATTATGATGGAGGGG+TGG | - | chr7.2:11085214-11085233 | MS.gene20064:CDS | 50.0% |
| ! | GAGCCAAAAAAGGGTGTACC+AGG | - | chr7.2:11085044-11085063 | MS.gene20064:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 11084879 | 11085379 | 11084879 | ID=MS.gene20064 |
| chr7.2 | mRNA | 11084879 | 11085379 | 11084879 | ID=MS.gene20064.t1;Parent=MS.gene20064 |
| chr7.2 | exon | 11084879 | 11085379 | 11084879 | ID=MS.gene20064.t1.exon1;Parent=MS.gene20064.t1 |
| chr7.2 | CDS | 11084879 | 11085379 | 11084879 | ID=cds.MS.gene20064.t1;Parent=MS.gene20064.t1 |
| Gene Sequence |
| Protein sequence |
