AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90981


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90981.t1 AT2G17870 64.789 71 25 0 2 72 7 77 7.50e-29 105
MS.gene90981.t1 AT2G21060 61.644 73 28 0 3 75 12 84 4.51e-28 101
MS.gene90981.t1 AT4G36020 60.563 71 28 0 2 72 7 77 2.88e-25 95.5
MS.gene90981.t1 AT4G36020 60.563 71 28 0 2 72 7 77 5.15e-25 95.5
MS.gene90981.t1 AT4G36020 60.563 71 28 0 2 72 7 77 5.15e-25 95.5
MS.gene90981.t1 AT4G38680 60.274 73 29 0 3 75 8 80 2.12e-22 86.7

Find 32 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAGTGGGTCAAAATTAAC+TGG 0.306323 4.2:+34826993 MS.gene90981:CDS
TTCAATGATCAGAAGGGGTT+TGG 0.339445 4.2:+34827029 MS.gene90981:CDS
AACTCCTCACTTCCATCATC+AGG 0.391515 4.2:-34827062 None:intergenic
TGCTACAGCTGTGGTGAATC+TGG 0.396062 4.2:+34827542 MS.gene90981:intron
CATCGGTTTGGATCTGAGAT+TGG 0.429198 4.2:-34827094 None:intergenic
TTTGCAAGAGATTGTCCAAC+AGG 0.433866 4.2:+34827569 MS.gene90981:CDS
AATTAACTGGGAAGGTGAAA+TGG 0.440654 4.2:+34827006 MS.gene90981:CDS
TCCAAGGCTGTTAGTGTGAC+TGG 0.440806 4.2:+34827182 MS.gene90981:CDS
GTGAGCGGAATCCATCGGTT+TGG 0.468363 4.2:-34827106 None:intergenic
GGATTCATCACCCCTGATGA+TGG 0.483119 4.2:+34827050 MS.gene90981:CDS
CTTCAACGAGCGCTGCCTGT+TGG 0.500201 4.2:-34827584 None:intergenic
TCCAGTCACACTAACAGCCT+TGG 0.511590 4.2:-34827183 None:intergenic
AGCAAGTGAGCGGAATCCAT+CGG 0.551382 4.2:-34827111 None:intergenic
GTTAGTGTGACTGGACCTGA+TGG 0.557938 4.2:+34827191 MS.gene90981:CDS
AAAATTCAATTCATCATGAG+TGG 0.563070 4.2:+34826978 None:intergenic
GAAATGGTTCAATGATCAGA+AGG 0.565831 4.2:+34827022 MS.gene90981:CDS
AGATCTGAGTAAAGTGGTGG+TGG 0.576919 4.2:+34827605 MS.gene90981:CDS
CGTTGAAGATCTGAGTAAAG+TGG 0.585840 4.2:+34827599 MS.gene90981:CDS
GGATTCCGCTCACTTGCTGA+AGG 0.587573 4.2:+34827116 MS.gene90981:CDS
ACTCCTCACTTCCATCATCA+GGG 0.589005 4.2:-34827061 None:intergenic
AAATTCAATTCATCATGAGT+GGG 0.603310 4.2:+34826979 None:intergenic
CAATCTCAGATCCAAACCGA+TGG 0.617544 4.2:+34827095 MS.gene90981:CDS
TGATAACGATGGAAGATCCA+AGG 0.617668 4.2:+34827166 MS.gene90981:CDS
AAATGGTTCAATGATCAGAA+GGG 0.619071 4.2:+34827023 MS.gene90981:CDS
CACCCCTGATGATGGAAGTG+AGG 0.619233 4.2:+34827058 MS.gene90981:CDS
AATGGTTCAATGATCAGAAG+GGG 0.619776 4.2:+34827024 MS.gene90981:CDS
TGGGTCAAAATTAACTGGGA+AGG 0.622767 4.2:+34826998 MS.gene90981:CDS
CAGATTGAGTCTGATAACGA+TGG 0.643460 4.2:+34827155 MS.gene90981:CDS
CTCCTCACTTCCATCATCAG+GGG 0.654781 4.2:-34827060 None:intergenic
ATTCACCTTCAGCAAGTGAG+CGG 0.656812 4.2:-34827121 None:intergenic
TGAGTGGGTCAAAATTAACT+GGG 0.660344 4.2:+34826994 MS.gene90981:CDS
TGAAGATCTGAGTAAAGTGG+TGG 0.733020 4.2:+34827602 MS.gene90981:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAATGGTTCAATGATCAGAA+GGG + chr4.2:34827023-34827042 MS.gene90981:CDS 30.0%
AATGGTTCAATGATCAGAAG+GGG + chr4.2:34827024-34827043 MS.gene90981:CDS 35.0%
AATTAACTGGGAAGGTGAAA+TGG + chr4.2:34827006-34827025 MS.gene90981:CDS 35.0%
ATGAGTGGGTCAAAATTAAC+TGG + chr4.2:34826993-34827012 MS.gene90981:CDS 35.0%
GAAATGGTTCAATGATCAGA+AGG + chr4.2:34827022-34827041 MS.gene90981:CDS 35.0%
TGAGTGGGTCAAAATTAACT+GGG + chr4.2:34826994-34827013 MS.gene90981:CDS 35.0%
CGTTGAAGATCTGAGTAAAG+TGG + chr4.2:34827599-34827618 MS.gene90981:CDS 40.0%
TGAAGATCTGAGTAAAGTGG+TGG + chr4.2:34827602-34827621 MS.gene90981:CDS 40.0%
TGGGTCAAAATTAACTGGGA+AGG + chr4.2:34826998-34827017 MS.gene90981:CDS 40.0%
TGTTACAACTGTGGTGAATC+TGG + chr4.2:34827431-34827450 MS.gene90981:intron 40.0%
TTCAATGATCAGAAGGGGTT+TGG + chr4.2:34827029-34827048 MS.gene90981:CDS 40.0%
TTTGCAAGAGATTGTCCAAC+AGG + chr4.2:34827569-34827588 MS.gene90981:CDS 40.0%
! CAGATTGAGTCTGATAACGA+TGG + chr4.2:34827155-34827174 MS.gene90981:CDS 40.0%
! TGATAACGATGGAAGATCCA+AGG + chr4.2:34827166-34827185 MS.gene90981:CDS 40.0%
AACTCCTCACTTCCATCATC+AGG - chr4.2:34827065-34827084 None:intergenic 45.0%
ACTCCTCACTTCCATCATCA+GGG - chr4.2:34827064-34827083 None:intergenic 45.0%
AGATCTGAGTAAAGTGGTGG+TGG + chr4.2:34827605-34827624 MS.gene90981:CDS 45.0%
ATTCACCTTCAGCAAGTGAG+CGG - chr4.2:34827124-34827143 None:intergenic 45.0%
CAATCTCAGATCCAAACCGA+TGG + chr4.2:34827095-34827114 MS.gene90981:CDS 45.0%
!! CATCGGTTTGGATCTGAGAT+TGG - chr4.2:34827097-34827116 None:intergenic 45.0%
AGCAAGTGAGCGGAATCCAT+CGG - chr4.2:34827114-34827133 None:intergenic 50.0%
CTCCTCACTTCCATCATCAG+GGG - chr4.2:34827063-34827082 None:intergenic 50.0%
GGATTCATCACCCCTGATGA+TGG + chr4.2:34827050-34827069 MS.gene90981:CDS 50.0%
GTTAGTGTGACTGGACCTGA+TGG + chr4.2:34827191-34827210 MS.gene90981:CDS 50.0%
TCCAAGGCTGTTAGTGTGAC+TGG + chr4.2:34827182-34827201 MS.gene90981:CDS 50.0%
TCCAGTCACACTAACAGCCT+TGG - chr4.2:34827186-34827205 None:intergenic 50.0%
TCTGTTCAAGGATCGAGACG+CGG + chr4.2:34827218-34827237 MS.gene90981:intron 50.0%
TGAATCTGGCCACATGGCAA+GGG + chr4.2:34827445-34827464 MS.gene90981:intron 50.0%
TGCTACAGCTGTGGTGAATC+TGG + chr4.2:34827542-34827561 MS.gene90981:intron 50.0%
! CCTGATGGTGCTTCTGTTCA+AGG + chr4.2:34827206-34827225 MS.gene90981:intron 50.0%
! TATGGAGGAGGTGGTGGTTA+TGG + chr4.2:34827365-34827384 MS.gene90981:intron 50.0%
!! CCTTGAACAGAAGCACCATC+AGG - chr4.2:34827209-34827228 None:intergenic 50.0%
!! GGTGGTGGATGTTACAACTG+TGG + chr4.2:34827422-34827441 MS.gene90981:intron 50.0%
CTGTGGTGAATCTGGCCACA+TGG + chr4.2:34827439-34827458 MS.gene90981:intron 55.0%
GGAGGTGGTTATGAACGTGG+TGG + chr4.2:34827254-34827273 MS.gene90981:intron 55.0%
GGTGGAGGTGGTTATGAACG+TGG + chr4.2:34827251-34827270 MS.gene90981:intron 55.0%
GGTGGTTATGAACGTGGTGG+TGG + chr4.2:34827257-34827276 MS.gene90981:intron 55.0%
GTGAATCTGGCCACATGGCA+AGG + chr4.2:34827444-34827463 MS.gene90981:intron 55.0%
TGGTACACTCCCTTGCCATG+TGG - chr4.2:34827457-34827476 None:intergenic 55.0%
! ATGGCAAGGGAGTGTACCAG+CGG + chr4.2:34827458-34827477 MS.gene90981:intron 55.0%
! GGATTCCGCTCACTTGCTGA+AGG + chr4.2:34827116-34827135 MS.gene90981:CDS 55.0%
! GGTGGTGGTTATGGTGGAAG+AGG + chr4.2:34827311-34827330 MS.gene90981:intron 55.0%
! GGTGGTTATGGTGGAAGAGG+AGG + chr4.2:34827314-34827333 MS.gene90981:intron 55.0%
! GGTTATGGTGGAAGAGGAGG+TGG + chr4.2:34827317-34827336 MS.gene90981:intron 55.0%
! GTGAGCGGAATCCATCGGTT+TGG - chr4.2:34827109-34827128 None:intergenic 55.0%
! TATGGTGGAAGAGGAGGTGG+TGG + chr4.2:34827320-34827339 MS.gene90981:intron 55.0%
!! ATGGTGGTGGTGGAAGAGGT+GGG + chr4.2:34827384-34827403 MS.gene90981:intron 55.0%
!! CACCCCTGATGATGGAAGTG+AGG + chr4.2:34827058-34827077 MS.gene90981:CDS 55.0%
!! GAACGTGGTGGTGGTGGTTA+CGG + chr4.2:34827266-34827285 MS.gene90981:intron 55.0%
!! GGTTATGAACGTGGTGGTGG+TGG + chr4.2:34827260-34827279 MS.gene90981:intron 55.0%
!! GGTTATGGTGGTGGTGGAAG+AGG + chr4.2:34827380-34827399 MS.gene90981:intron 55.0%
!! TATGGTGGTGGTGGAAGAGG+TGG + chr4.2:34827383-34827402 MS.gene90981:intron 55.0%
GGAGGTGGAGGTGGTTATGG+AGG + chr4.2:34827350-34827369 MS.gene90981:intron 60.0%
GGAGGTGGTTATGGAGGAGG+TGG + chr4.2:34827356-34827375 MS.gene90981:intron 60.0%
GGTGGAAGCTGCTACAGCTG+TGG + chr4.2:34827533-34827552 MS.gene90981:intron 60.0%
GGTGGAGGTGGAGGTGGTTA+TGG + chr4.2:34827347-34827366 MS.gene90981:intron 60.0%
GGTGGAGGTGGTTATGGAGG+AGG + chr4.2:34827353-34827372 MS.gene90981:intron 60.0%
GGTGGTTATGGAGGAGGTGG+TGG + chr4.2:34827359-34827378 MS.gene90981:intron 60.0%
GTTCAAGGATCGAGACGCGG+AGG + chr4.2:34827221-34827240 MS.gene90981:intron 60.0%
! AGAGGAGGTGGTGGCTATGG+TGG + chr4.2:34827329-34827348 MS.gene90981:intron 60.0%
! CTTCAACGAGCGCTGCCTGT+TGG - chr4.2:34827587-34827606 None:intergenic 60.0%
! GGAAGAGGAGGTGGTGGCTA+TGG + chr4.2:34827326-34827345 MS.gene90981:intron 60.0%
! GGAAGGTATGGAGGTGGTGG+TGG + chr4.2:34827497-34827516 MS.gene90981:intron 60.0%
! GGAGGAGGTGGTGGTTATGG+TGG + chr4.2:34827368-34827387 MS.gene90981:intron 60.0%
! GGAGGTGGTGGTTATGGTGG+TGG + chr4.2:34827308-34827327 MS.gene90981:intron 60.0%
! GGTGGAAGGTATGGAGGTGG+TGG + chr4.2:34827494-34827513 MS.gene90981:intron 60.0%
! GGTGGAGGTGGTGGTTATGG+TGG + chr4.2:34827305-34827324 MS.gene90981:intron 60.0%
!! AGGTATGGAGGTGGTGGTGG+TGG + chr4.2:34827500-34827519 MS.gene90981:intron 60.0%
!! GGTGGTGGAGGTGGTGGTTA+TGG + chr4.2:34827302-34827321 MS.gene90981:intron 60.0%
!! GGTGGTGGTTATGGTGGTGG+TGG + chr4.2:34827374-34827393 MS.gene90981:intron 60.0%
!! GGTTACGGTGGTGGCGGTTA+TGG + chr4.2:34827281-34827300 MS.gene90981:intron 60.0%
!! GGTTATGGTGGTGGAGGTGG+TGG + chr4.2:34827296-34827315 MS.gene90981:intron 60.0%
!! TACGGTGGTGGCGGTTATGG+TGG + chr4.2:34827284-34827303 MS.gene90981:intron 60.0%
!! TATGGAGGTGGTGGTGGTGG+AGG + chr4.2:34827503-34827522 MS.gene90981:intron 60.0%
!! TGGTGGTGGTGGAAGAGGTG+GGG + chr4.2:34827385-34827404 MS.gene90981:intron 60.0%
ATCCACCACCGCCTCCTCCA+CGG - chr4.2:34827412-34827431 None:intergenic 65.0%
CAAGGATCGAGACGCGGAGG+CGG + chr4.2:34827224-34827243 MS.gene90981:intron 65.0%
GAGTGTACCAGCGGTGGAGG+TGG + chr4.2:34827467-34827486 MS.gene90981:intron 65.0%
TGTACCAGCGGTGGAGGTGG+TGG + chr4.2:34827470-34827489 MS.gene90981:intron 65.0%
! AGGGAGTGTACCAGCGGTGG+AGG + chr4.2:34827464-34827483 MS.gene90981:intron 65.0%
! GCAAGGGAGTGTACCAGCGG+TGG + chr4.2:34827461-34827480 MS.gene90981:intron 65.0%
! GGAGGTGGTGGCTATGGTGG+AGG + chr4.2:34827332-34827351 MS.gene90981:intron 65.0%
! GGCGGTGGAAGGTATGGAGG+TGG + chr4.2:34827491-34827510 MS.gene90981:intron 65.0%
! GGCTATGGTGGAGGTGGAGG+TGG + chr4.2:34827341-34827360 MS.gene90981:intron 65.0%
! GGTGGCGGTGGAAGGTATGG+AGG + chr4.2:34827488-34827507 MS.gene90981:intron 65.0%
! GGTGGCTATGGTGGAGGTGG+AGG + chr4.2:34827338-34827357 MS.gene90981:intron 65.0%
! GGTGGTGGCTATGGTGGAGG+TGG + chr4.2:34827335-34827354 MS.gene90981:intron 65.0%
! GGTGGTGGTTACGGTGGTGG+CGG + chr4.2:34827275-34827294 MS.gene90981:intron 65.0%
!! CGTGGTGGTGGTGGTTACGG+TGG + chr4.2:34827269-34827288 MS.gene90981:intron 65.0%
!! GGCGGTTATGGTGGTGGAGG+TGG + chr4.2:34827293-34827312 MS.gene90981:intron 65.0%
!! GGTGGCGGTTATGGTGGTGG+AGG + chr4.2:34827290-34827309 MS.gene90981:intron 65.0%
!! GGTGGTGGCGGTGGAAGGTA+TGG + chr4.2:34827485-34827504 MS.gene90981:intron 65.0%
!! GGTGGTGGCGGTTATGGTGG+TGG + chr4.2:34827287-34827306 MS.gene90981:intron 65.0%
!! GGTGGTGGTGGAAGAGGTGG+GGG + chr4.2:34827386-34827405 MS.gene90981:intron 65.0%
!! GGTGGTGGTGGTTACGGTGG+TGG + chr4.2:34827272-34827291 MS.gene90981:intron 65.0%
! ACCAGCGGTGGAGGTGGTGG+CGG + chr4.2:34827473-34827492 MS.gene90981:intron 70.0%
!! GGTGGTGGTGGAGGCAGTGG+CGG + chr4.2:34827512-34827531 MS.gene90981:intron 70.0%
!! GGTGGTGGTGGTGGAGGCAG+TGG + chr4.2:34827509-34827528 MS.gene90981:intron 70.0%
!! GTGGAGGTGGTGGCGGTGGA+AGG + chr4.2:34827480-34827499 MS.gene90981:intron 70.0%
ACCGCCACCACCTCCACCGC+TGG - chr4.2:34827477-34827496 None:intergenic 75.0%
AGAGGTGGGGGCCGTGGAGG+AGG + chr4.2:34827398-34827417 MS.gene90981:intron 75.0%
GGAAGAGGTGGGGGCCGTGG+AGG + chr4.2:34827395-34827414 MS.gene90981:intron 75.0%
GGATCGAGACGCGGAGGCGG+TGG + chr4.2:34827227-34827246 MS.gene90981:intron 75.0%
GGTGGAAGAGGTGGGGGCCG+TGG + chr4.2:34827392-34827411 MS.gene90981:intron 75.0%
TCGAGACGCGGAGGCGGTGG+TGG + chr4.2:34827230-34827249 MS.gene90981:intron 75.0%
! AGACGCGGAGGCGGTGGTGG+TGG + chr4.2:34827233-34827252 MS.gene90981:intron 75.0%
!! AGCGGTGGAGGTGGTGGCGG+TGG + chr4.2:34827476-34827495 MS.gene90981:intron 75.0%
!! GGAGGCGGTGGTGGTGGAGG+TGG + chr4.2:34827239-34827258 MS.gene90981:intron 75.0%
!! GGTGGTGGAGGCAGTGGCGG+TGG + chr4.2:34827515-34827534 MS.gene90981:intron 75.0%
! GGCCGTGGAGGAGGCGGTGG+TGG + chr4.2:34827407-34827426 MS.gene90981:intron 80.0%
! GGTGGGGGCCGTGGAGGAGG+CGG + chr4.2:34827401-34827420 MS.gene90981:intron 80.0%
!!! CGCGGAGGCGGTGGTGGTGG+AGG + chr4.2:34827236-34827255 MS.gene90981:intron 80.0%
! GGGGGCCGTGGAGGAGGCGG+TGG + chr4.2:34827404-34827423 MS.gene90981:intron 85.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 34826993 34827632 34826993 ID=MS.gene90981
chr4.2 mRNA 34826993 34827632 34826993 ID=MS.gene90981.t1;Parent=MS.gene90981
chr4.2 exon 34826993 34827212 34826993 ID=MS.gene90981.t1.exon1;Parent=MS.gene90981.t1
chr4.2 CDS 34826993 34827212 34826993 ID=cds.MS.gene90981.t1;Parent=MS.gene90981.t1
chr4.2 exon 34827550 34827632 34827550 ID=MS.gene90981.t1.exon2;Parent=MS.gene90981.t1
chr4.2 CDS 34827550 34827632 34827550 ID=cds.MS.gene90981.t1;Parent=MS.gene90981.t1
Gene Sequence

>MS.gene90981

ATGAGTGGGTCAAAATTAACTGGGAAGGTGAAATGGTTCAATGATCAGAAGGGGTTTGGATTCATCACCCCTGATGATGGAAGTGAGGAGTTGTTTGTTCACCAATCTCAGATCCAAACCGATGGATTCCGCTCACTTGCTGAAGGTGAATCTGTTGAGTATCAGATTGAGTCTGATAACGATGGAAGATCCAAGGCTGTTAGTGTGACTGGACCTGATGGTGCTTCTGTTCAAGGATCGAGACGCGGAGGCGGTGGTGGTGGAGGTGGTTATGAACGTGGTGGTGGTGGTTACGGTGGTGGCGGTTATGGTGGTGGAGGTGGTGGTTATGGTGGAAGAGGAGGTGGTGGCTATGGTGGAGGTGGAGGTGGTTATGGAGGAGGTGGTGGTTATGGTGGTGGTGGAAGAGGTGGGGGCCGTGGAGGAGGCGGTGGTGGATGTTACAACTGTGGTGAATCTGGCCACATGGCAAGGGAGTGTACCAGCGGTGGAGGTGGTGGCGGTGGAAGGTATGGAGGTGGTGGTGGTGGAGGCAGTGGCGGTGGAAGCTGCTACAGCTGTGGTGAATCTGGTCATTTTGCAAGAGATTGTCCAACAGGCAGCGCTCGTTGAAGATCTGAGTAAAGTGGTGGTGGTTTAG

Protein sequence

>MS.gene90981.t1

MSGSKLTGKVKWFNDQKGFGFITPDDGSEELFVHQSQIQTDGFRSLAEGESVEYQIESDNDGRSKAVSVTGPDAVVNLVILQEIVQQAALVEDLSKVVVV