AlfalfaGEDB | MS.gene64650

M. sativa cultivar XinJiangDaYe / MS.gene64650

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64650.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64650.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64650.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene64650	0.826264	2.98E-54	-1.69E-46
MS.gene049159	MS.gene64650	0.845954	2.94E-59	-1.69E-46
MS.gene049255	MS.gene64650	0.827299	1.69E-54	-1.69E-46
MS.gene049341	MS.gene64650	0.83983	1.25E-57	-1.69E-46
MS.gene049404	MS.gene64650	0.810701	1.01E-50	-1.69E-46
MS.gene049821	MS.gene64650	0.887909	9.43E-73	-1.69E-46
MS.gene049850	MS.gene64650	0.83496	2.22E-56	-1.69E-46
MS.gene050020	MS.gene64650	0.866624	2.49E-65	-1.69E-46
MS.gene050237	MS.gene64650	0.803282	3.75E-49	-1.69E-46
MS.gene050277	MS.gene64650	0.801075	1.07E-48	-1.69E-46
MS.gene050303	MS.gene64650	0.822424	2.39E-53	-1.69E-46
MS.gene050442	MS.gene64650	0.824811	6.59E-54	-1.69E-46
MS.gene05045	MS.gene64650	0.819613	1.06E-52	-1.69E-46
MS.gene050689	MS.gene64650	0.816909	4.34E-52	-1.69E-46
MS.gene050719	MS.gene64650	0.820501	6.63E-53	-1.69E-46
MS.gene050721	MS.gene64650	0.815929	7.19E-52	-1.69E-46
MS.gene050724	MS.gene64650	0.816549	5.22E-52	-1.69E-46
MS.gene050807	MS.gene64650	0.851288	9.78E-61	-1.69E-46
MS.gene050808	MS.gene64650	0.826818	2.20E-54	-1.69E-46
MS.gene050815	MS.gene64650	0.845505	3.89E-59	-1.69E-46
MS.gene050901	MS.gene64650	0.848555	5.69E-60	-1.69E-46
MS.gene051243	MS.gene64650	0.813947	1.98E-51	-1.69E-46
MS.gene051247	MS.gene64650	0.846856	1.67E-59	-1.69E-46
MS.gene051328	MS.gene64650	0.87072	1.18E-66	-1.69E-46
MS.gene05134	MS.gene64650	0.820643	6.15E-53	-1.69E-46
MS.gene05157	MS.gene64650	0.853105	2.98E-61	-1.69E-46
MS.gene051594	MS.gene64650	0.80442	2.18E-49	-1.69E-46
MS.gene051700	MS.gene64650	0.826759	2.27E-54	-1.69E-46
MS.gene051719	MS.gene64650	0.861293	1.14E-63	-1.69E-46
MS.gene051720	MS.gene64650	0.849301	3.53E-60	-1.69E-46
MS.gene051744	MS.gene64650	0.833932	4.01E-56	-1.69E-46
MS.gene051957	MS.gene64650	0.806644	7.44E-50	-1.69E-46
MS.gene051975	MS.gene64650	0.816336	5.83E-52	-1.69E-46
MS.gene052012	MS.gene64650	0.832394	9.69E-56	-1.69E-46
MS.gene052020	MS.gene64650	0.835297	1.82E-56	-1.69E-46
MS.gene052133	MS.gene64650	0.860547	1.91E-63	-1.69E-46
MS.gene052323	MS.gene64650	0.808692	2.73E-50	-1.69E-46
MS.gene052516	MS.gene64650	0.845363	4.26E-59	-1.69E-46
MS.gene052519	MS.gene64650	0.826399	2.77E-54	-1.69E-46
MS.gene052582	MS.gene64650	0.802543	5.34E-49	-1.69E-46
MS.gene052584	MS.gene64650	0.809339	1.99E-50	-1.69E-46
MS.gene052611	MS.gene64650	0.814137	1.80E-51	-1.69E-46
MS.gene052665	MS.gene64650	0.83054	2.77E-55	-1.69E-46
MS.gene052773	MS.gene64650	0.81681	4.57E-52	-1.69E-46
MS.gene052863	MS.gene64650	0.813568	2.40E-51	-1.69E-46
MS.gene052896	MS.gene64650	0.800398	1.47E-48	-1.69E-46
MS.gene052947	MS.gene64650	0.825544	4.43E-54	-1.69E-46
MS.gene053008	MS.gene64650	0.834657	2.64E-56	-1.69E-46
MS.gene053074	MS.gene64650	0.806955	6.40E-50	-1.69E-46
MS.gene053226	MS.gene64650	0.865823	4.47E-65	-1.69E-46
MS.gene053563	MS.gene64650	0.817986	2.48E-52	-1.69E-46
MS.gene053581	MS.gene64650	0.837226	5.89E-57	-1.69E-46
MS.gene053599	MS.gene64650	0.815693	8.11E-52	-1.69E-46
MS.gene053630	MS.gene64650	0.804863	1.76E-49	-1.69E-46
MS.gene05364	MS.gene64650	0.81487	1.24E-51	-1.69E-46
MS.gene053883	MS.gene64650	0.802023	6.83E-49	-1.69E-46
MS.gene054236	MS.gene64650	0.814567	1.44E-51	-1.69E-46
MS.gene05462	MS.gene64650	0.826931	2.07E-54	-1.69E-46
MS.gene054651	MS.gene64650	0.813285	2.77E-51	-1.69E-46
MS.gene054775	MS.gene64650	0.806569	7.72E-50	-1.69E-46
MS.gene05517	MS.gene64650	0.813554	2.41E-51	-1.69E-46
MS.gene055465	MS.gene64650	0.847732	9.60E-60	-1.69E-46
MS.gene055466	MS.gene64650	0.875178	3.78E-68	-1.69E-46
MS.gene055468	MS.gene64650	0.864608	1.08E-64	-1.69E-46
MS.gene055469	MS.gene64650	0.809918	1.49E-50	-1.69E-46
MS.gene055512	MS.gene64650	0.826335	2.87E-54	-1.69E-46
MS.gene055513	MS.gene64650	0.801382	9.25E-49	-1.69E-46
MS.gene055580	MS.gene64650	-0.816701	4.83E-52	-1.69E-46
MS.gene055608	MS.gene64650	0.828514	8.62E-55	-1.69E-46
MS.gene055623	MS.gene64650	0.810197	1.30E-50	-1.69E-46
MS.gene055778	MS.gene64650	0.840415	8.81E-58	-1.69E-46
MS.gene055808	MS.gene64650	0.81917	1.34E-52	-1.69E-46
MS.gene055818	MS.gene64650	0.837518	4.96E-57	-1.69E-46
MS.gene055912	MS.gene64650	0.802842	4.63E-49	-1.69E-46
MS.gene056043	MS.gene64650	-0.800847	1.19E-48	-1.69E-46
MS.gene05608	MS.gene64650	0.812005	5.27E-51	-1.69E-46
MS.gene056173	MS.gene64650	0.800741	1.25E-48	-1.69E-46
MS.gene056174	MS.gene64650	0.833479	5.21E-56	-1.69E-46
MS.gene056215	MS.gene64650	0.893959	3.86E-75	-1.69E-46
MS.gene056308	MS.gene64650	0.812898	3.36E-51	-1.69E-46
MS.gene056366	MS.gene64650	0.82176	3.40E-53	-1.69E-46
MS.gene056580	MS.gene64650	0.801959	7.04E-49	-1.69E-46
MS.gene056595	MS.gene64650	0.826415	2.75E-54	-1.69E-46
MS.gene056631	MS.gene64650	0.821494	3.92E-53	-1.69E-46
MS.gene056760	MS.gene64650	0.817667	2.93E-52	-1.69E-46
MS.gene057057	MS.gene64650	0.805341	1.40E-49	-1.69E-46
MS.gene057060	MS.gene64650	0.820549	6.47E-53	-1.69E-46
MS.gene057314	MS.gene64650	0.821975	3.03E-53	-1.69E-46
MS.gene057444	MS.gene64650	0.8318	1.36E-55	-1.69E-46
MS.gene057446	MS.gene64650	0.800186	1.62E-48	-1.69E-46
MS.gene057693	MS.gene64650	0.86997	2.08E-66	-1.69E-46
MS.gene057694	MS.gene64650	0.801476	8.84E-49	-1.69E-46
MS.gene057705	MS.gene64650	0.825303	5.05E-54	-1.69E-46
MS.gene057915	MS.gene64650	0.833623	4.80E-56	-1.69E-46
MS.gene058010	MS.gene64650	0.813133	2.99E-51	-1.69E-46
MS.gene058084	MS.gene64650	0.827095	1.89E-54	-1.69E-46
MS.gene058168	MS.gene64650	0.81679	4.61E-52	-1.69E-46
MS.gene05847	MS.gene64650	0.834528	2.85E-56	-1.69E-46
MS.gene058548	MS.gene64650	0.809338	1.99E-50	-1.69E-46
MS.gene058625	MS.gene64650	0.819785	9.68E-53	-1.69E-46
MS.gene058636	MS.gene64650	0.807932	3.97E-50	-1.69E-46
MS.gene058906	MS.gene64650	0.827739	1.32E-54	-1.69E-46
MS.gene059006	MS.gene64650	0.809987	1.44E-50	-1.69E-46
MS.gene059151	MS.gene64650	0.819196	1.32E-52	-1.69E-46
MS.gene05954	MS.gene64650	0.805993	1.02E-49	-1.69E-46
MS.gene059625	MS.gene64650	0.821987	3.01E-53	-1.69E-46
MS.gene059725	MS.gene64650	0.800477	1.41E-48	-1.69E-46
MS.gene05978	MS.gene64650	0.818988	1.47E-52	-1.69E-46
MS.gene059842	MS.gene64650	0.841028	6.09E-58	-1.69E-46
MS.gene059937	MS.gene64650	0.83413	3.58E-56	-1.69E-46
MS.gene06001	MS.gene64650	0.813425	2.58E-51	-1.69E-46
MS.gene060310	MS.gene64650	0.819807	9.57E-53	-1.69E-46
MS.gene060429	MS.gene64650	0.869533	2.88E-66	-1.69E-46
MS.gene060587	MS.gene64650	0.852925	3.35E-61	-1.69E-46
MS.gene060803	MS.gene64650	0.858955	5.77E-63	-1.69E-46
MS.gene060907	MS.gene64650	0.814091	1.84E-51	-1.69E-46
MS.gene060917	MS.gene64650	0.836979	6.81E-57	-1.69E-46
MS.gene061176	MS.gene64650	0.814466	1.52E-51	-1.69E-46
MS.gene061177	MS.gene64650	0.813892	2.03E-51	-1.69E-46
MS.gene061178	MS.gene64650	0.814207	1.73E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene022946	MS.gene64650	PPI
MS.gene018580	MS.gene64650	PPI
MS.gene04064	MS.gene64650	PPI
MS.gene64650	MS.gene89488	PPI
MS.gene061876	MS.gene64650	PPI
MS.gene05619	MS.gene64650	PPI
MS.gene05608	MS.gene64650	PPI
MS.gene022932	MS.gene64650	PPI
MS.gene012391	MS.gene64650	PPI
MS.gene88681	MS.gene64650	PPI
MS.gene070244	MS.gene64650	PPI
MS.gene035897	MS.gene64650	PPI
MS.gene64920	MS.gene64650	PPI
MS.gene060463	MS.gene64650	PPI
MS.gene070907	MS.gene64650	PPI
MS.gene60093	MS.gene64650	PPI
MS.gene004124	MS.gene64650	PPI
MS.gene018578	MS.gene64650	PPI
MS.gene31196	MS.gene64650	PPI
MS.gene89488	MS.gene64650	PPI
MS.gene010219	MS.gene64650	PPI
MS.gene060465	MS.gene64650	PPI
MS.gene64650	MS.gene68545	PPI
MS.gene061867	MS.gene64650	PPI
MS.gene64650	MS.gene016624	PPI
MS.gene08121	MS.gene64650	PPI
MS.gene061870	MS.gene64650	PPI
MS.gene035718	MS.gene64650	PPI
MS.gene035896	MS.gene64650	PPI
MS.gene73136	MS.gene64650	PPI
MS.gene008276	MS.gene64650	PPI
MS.gene009027	MS.gene64650	PPI
MS.gene64650	MS.gene049705	PPI
MS.gene09873	MS.gene64650	PPI
MS.gene78578	MS.gene64650	PPI
MS.gene035721	MS.gene64650	PPI
MS.gene04052	MS.gene64650	PPI
MS.gene08122	MS.gene64650	PPI
MS.gene048705	MS.gene64650	PPI
MS.gene05623	MS.gene64650	PPI
MS.gene043967	MS.gene64650	PPI
MS.gene84536	MS.gene64650	PPI
MS.gene04972	MS.gene64650	PPI
MS.gene028629	MS.gene64650	PPI
MS.gene82546	MS.gene64650	PPI
MS.gene61017	MS.gene64650	PPI
MS.gene04433	MS.gene64650	PPI
MS.gene64650	MS.gene60093	PPI
MS.gene035787	MS.gene64650	PPI
MS.gene04067	MS.gene64650	PPI
MS.gene71215	MS.gene64650	PPI
MS.gene061874	MS.gene64650	PPI
MS.gene061866	MS.gene64650	PPI
MS.gene004121	MS.gene64650	PPI
MS.gene035719	MS.gene64650	PPI
MS.gene58854	MS.gene64650	PPI
MS.gene64650	MS.gene04972	PPI
MS.gene66225	MS.gene64650	PPI
MS.gene70955	MS.gene64650	PPI
MS.gene071136	MS.gene64650	PPI
MS.gene040383	MS.gene64650	PPI
MS.gene070242	MS.gene64650	PPI
MS.gene072493	MS.gene64650	PPI
MS.gene035720	MS.gene64650	PPI
MS.gene012390	MS.gene64650	PPI
MS.gene048746	MS.gene64650	PPI
MS.gene64650	MS.gene018578	PPI
MS.gene010220	MS.gene64650	PPI
MS.gene79195	MS.gene64650	PPI
MS.gene64650	MS.gene018580	PPI
MS.gene64650	MS.gene004124	PPI
MS.gene88680	MS.gene64650	PPI
MS.gene68545	MS.gene64650	PPI
MS.gene056215	MS.gene64650	PPI
MS.gene061869	MS.gene64650	PPI
MS.gene064557	MS.gene64650	PPI
MS.gene049821	MS.gene64650	PPI
MS.gene62520	MS.gene64650	PPI
MS.gene038469	MS.gene64650	PPI
MS.gene04452	MS.gene64650	PPI
MS.gene049705	MS.gene64650	PPI
MS.gene64650	MS.gene061874	PPI
MS.gene09871	MS.gene64650	PPI
MS.gene04576	MS.gene64650	PPI
MS.gene89487	MS.gene64650	PPI
MS.gene016624	MS.gene64650	PPI
MS.gene64650	MS.gene004121	PPI
MS.gene04392	MS.gene64650	PPI
MS.gene30920	MS.gene64650	PPI
MS.gene08053	MS.gene64650	PPI
MS.gene061872	MS.gene64650	PPI
MS.gene04068	MS.gene64650	PPI
MS.gene070241	MS.gene64650	PPI
MS.gene04434	MS.gene64650	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64650.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64650.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64650.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64650.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64650.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64650.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64650.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64650.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64650.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64650.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64650.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64650.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64650.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64650.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene64650.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene64650.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64650.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64650.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64650.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64650.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64650.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64650.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64650.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64650.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64650.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64650.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene64650.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 38 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGAACCCTCTACGGATTT+GGG	0.267091	4.4:-43367605	MS.gene64650:CDS
GGAAGAACCCTCTACGGATT+TGG	0.284984	4.4:-43367606	MS.gene64650:CDS
TGATTTCTTATCAAGATGTC+TGG	0.292016	4.4:-43367903	None:intergenic
CTTCTTGGAGAATGTGATTA+GGG	0.347463	4.4:-43367706	MS.gene64650:CDS
TCTTCTTGGAGAATGTGATT+AGG	0.365922	4.4:-43367707	MS.gene64650:CDS
GGAAAGGGTTTGGGAAAGGG+TGG	0.366216	4.4:-43367867	MS.gene64650:CDS
TGGCGTGTTGAAGATCTTCT+TGG	0.370355	4.4:-43367721	MS.gene64650:CDS
GGAAAAGGAGGAAAGGGTTT+GGG	0.378357	4.4:-43367876	MS.gene64650:CDS
TATCAGTGGTTTGATTTATG+AGG	0.387367	4.4:-43367751	MS.gene64650:CDS
TGGAAAAGGAGGAAAGGGTT+TGG	0.387838	4.4:-43367877	MS.gene64650:CDS
AAGATGTCTGGGCGTGGAAA+AGG	0.434173	4.4:-43367891	MS.gene64650:CDS
TCGCTGGCTTTGTGATTCCC+TGG	0.442319	4.4:+43367808	None:intergenic
GGAGGAAAGGGTTTGGGAAA+GGG	0.445686	4.4:-43367870	MS.gene64650:CDS
AGGAGGAAAGGGTTTGGGAA+AGG	0.475381	4.4:-43367871	MS.gene64650:CDS
CTTACACTGAGCACGCTAGA+AGG	0.481731	4.4:-43367674	MS.gene64650:CDS
GACTGTTACCGCCATGGATG+TGG	0.483826	4.4:-43367649	MS.gene64650:CDS
GGTTTATGCTCTCAAGAGAC+AGG	0.499176	4.4:-43367628	MS.gene64650:CDS
GGTGCTTCGTGACAACATCC+AGG	0.512203	4.4:-43367826	MS.gene64650:CDS
GATTTCTTATCAAGATGTCT+GGG	0.518762	4.4:-43367902	None:intergenic
CGAGCAAGACGACGAATCGC+TGG	0.534933	4.4:+43367792	None:intergenic
TGTTGTCACGAAGCACCTTA+CGG	0.540622	4.4:+43367832	None:intergenic
CGTCGTCTTGCTCGTAGAGG+AGG	0.557881	4.4:-43367783	MS.gene64650:CDS
AAGAACCCTCTACGGATTTG+GGG	0.565022	4.4:-43367604	MS.gene64650:CDS
TGGAGCAAAACGACACCGTA+AGG	0.575994	4.4:-43367847	MS.gene64650:CDS
CTTATCAAGATGTCTGGGCG+TGG	0.577382	4.4:-43367897	None:intergenic
GGGCGTGGAAAAGGAGGAAA+GGG	0.587932	4.4:-43367882	MS.gene64650:CDS
AAGGAAGACTGTTACCGCCA+TGG	0.588179	4.4:-43367655	MS.gene64650:CDS
GGAGGAGTGAAGCGTATCAG+TGG	0.591400	4.4:-43367765	MS.gene64650:CDS
ATGTCTGGGCGTGGAAAAGG+AGG	0.593086	4.4:-43367888	MS.gene64650:CDS
AGACAGGGAAGAACCCTCTA+CGG	0.600202	4.4:-43367612	MS.gene64650:CDS
TGGGCGTGGAAAAGGAGGAA+AGG	0.624585	4.4:-43367883	MS.gene64650:CDS
GAGAGCATAAACCACATCCA+TGG	0.625099	4.4:+43367638	None:intergenic
AGAACCCTCTACGGATTTGG+GGG	0.641482	4.4:-43367603	MS.gene64650:CDS
TTGATTTATGAGGAGACACG+TGG	0.648281	4.4:-43367741	MS.gene64650:CDS
GTGCTTCGTGACAACATCCA+GGG	0.652644	4.4:-43367825	MS.gene64650:CDS
ATTCGTCGTCTTGCTCGTAG+AGG	0.654201	4.4:-43367786	MS.gene64650:CDS
GTTTATGCTCTCAAGAGACA+GGG	0.674567	4.4:-43367627	MS.gene64650:CDS
AGCATAAACCACATCCATGG+CGG	0.701499	4.4:+43367641	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TATCAGTGGTTTGATTTATG+AGG	-	chr4.4:43367736-43367755	MS.gene64650:CDS	30.0%
	CTTCTTGGAGAATGTGATTA+GGG	-	chr4.4:43367781-43367800	MS.gene64650:CDS	35.0%
	TCTTCTTGGAGAATGTGATT+AGG	-	chr4.4:43367780-43367799	MS.gene64650:CDS	35.0%
	GTTTATGCTCTCAAGAGACA+GGG	-	chr4.4:43367860-43367879	MS.gene64650:CDS	40.0%
	TTGATTTATGAGGAGACACG+TGG	-	chr4.4:43367746-43367765	MS.gene64650:CDS	40.0%
	AAGAACCCTCTACGGATTTG+GGG	-	chr4.4:43367883-43367902	MS.gene64650:CDS	45.0%
	AGCATAAACCACATCCATGG+CGG	+	chr4.4:43367849-43367868	None:intergenic	45.0%
	GAAGAACCCTCTACGGATTT+GGG	-	chr4.4:43367882-43367901	MS.gene64650:CDS	45.0%
	GGAAAAGGAGGAAAGGGTTT+GGG	-	chr4.4:43367611-43367630	MS.gene64650:CDS	45.0%
	GGTTTATGCTCTCAAGAGAC+AGG	-	chr4.4:43367859-43367878	MS.gene64650:CDS	45.0%
	TGGAAAAGGAGGAAAGGGTT+TGG	-	chr4.4:43367610-43367629	MS.gene64650:CDS	45.0%
	TGGCGTGTTGAAGATCTTCT+TGG	-	chr4.4:43367766-43367785	MS.gene64650:CDS	45.0%
!	GAGAGCATAAACCACATCCA+TGG	+	chr4.4:43367852-43367871	None:intergenic	45.0%
!	TGTTGTCACGAAGCACCTTA+CGG	+	chr4.4:43367658-43367677	None:intergenic	45.0%
	AAGGAAGACTGTTACCGCCA+TGG	-	chr4.4:43367832-43367851	MS.gene64650:CDS	50.0%
	AGAACCCTCTACGGATTTGG+GGG	-	chr4.4:43367884-43367903	MS.gene64650:CDS	50.0%
	AGACAGGGAAGAACCCTCTA+CGG	-	chr4.4:43367875-43367894	MS.gene64650:CDS	50.0%
	AGGAGGAAAGGGTTTGGGAA+AGG	-	chr4.4:43367616-43367635	MS.gene64650:CDS	50.0%
	ATTCGTCGTCTTGCTCGTAG+AGG	-	chr4.4:43367701-43367720	MS.gene64650:CDS	50.0%
	CTAACCCCCAAATCCGTAGA+GGG	+	chr4.4:43367891-43367910	None:intergenic	50.0%
	CTTACACTGAGCACGCTAGA+AGG	-	chr4.4:43367813-43367832	MS.gene64650:CDS	50.0%
	GGAAGAACCCTCTACGGATT+TGG	-	chr4.4:43367881-43367900	MS.gene64650:CDS	50.0%
	GGAGGAAAGGGTTTGGGAAA+GGG	-	chr4.4:43367617-43367636	MS.gene64650:CDS	50.0%
	GTGCTTCGTGACAACATCCA+GGG	-	chr4.4:43367662-43367681	MS.gene64650:CDS	50.0%
	TGGAGCAAAACGACACCGTA+AGG	-	chr4.4:43367640-43367659	MS.gene64650:CDS	50.0%
	ATGTCTGGGCGTGGAAAAGG+AGG	-	chr4.4:43367599-43367618	MS.gene64650:CDS	55.0%
	GACTGTTACCGCCATGGATG+TGG	-	chr4.4:43367838-43367857	MS.gene64650:CDS	55.0%
	GGAAAGGGTTTGGGAAAGGG+TGG	-	chr4.4:43367620-43367639	MS.gene64650:CDS	55.0%
	GGAGGAGTGAAGCGTATCAG+TGG	-	chr4.4:43367722-43367741	MS.gene64650:CDS	55.0%
	GGGCGTGGAAAAGGAGGAAA+GGG	-	chr4.4:43367605-43367624	MS.gene64650:CDS	55.0%
	GGTGCTTCGTGACAACATCC+AGG	-	chr4.4:43367661-43367680	MS.gene64650:CDS	55.0%
	TGGGCGTGGAAAAGGAGGAA+AGG	-	chr4.4:43367604-43367623	MS.gene64650:CDS	55.0%
!!	TCGCTGGCTTTGTGATTCCC+TGG	+	chr4.4:43367682-43367701	None:intergenic	55.0%
	CGAGCAAGACGACGAATCGC+TGG	+	chr4.4:43367698-43367717	None:intergenic	60.0%
	CGTCGTCTTGCTCGTAGAGG+AGG	-	chr4.4:43367704-43367723	MS.gene64650:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	43367599	43367910	43367599	ID=MS.gene64650
chr4.4	mRNA	43367599	43367910	43367599	ID=MS.gene64650.t1;Parent=MS.gene64650
chr4.4	exon	43367599	43367910	43367599	ID=MS.gene64650.t1.exon1;Parent=MS.gene64650.t1
chr4.4	CDS	43367599	43367910	43367599	ID=cds.MS.gene64650.t1;Parent=MS.gene64650.t1

Gene Sequence	>MS.gene64650 ATGTCTGGGCGTGGAAAAGGAGGAAAGGGTTTGGGAAAGGGTGGAGCAAAACGACACCGTAAGGTGCTTCGTGACAACATCCAGGGAATCACAAAGCCAGCGATTCGTCGTCTTGCTCGTAGAGGAGGAGTGAAGCGTATCAGTGGTTTGATTTATGAGGAGACACGTGGCGTGTTGAAGATCTTCTTGGAGAATGTGATTAGGGATGCTGTGACTTACACTGAGCACGCTAGAAGGAAGACTGTTACCGCCATGGATGTGGTTTATGCTCTCAAGAGACAGGGAAGAACCCTCTACGGATTTGGGGGTTAG
Protein sequence	>MS.gene64650.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG