AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35048


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35048.t1 MTR_4g070140 59.524 84 34 0 2 85 3 86 9.55e-30 109
MS.gene35048.t1 MTR_4g070080 58.228 79 33 0 6 84 5 83 1.21e-28 105
MS.gene35048.t1 MTR_4g070190 55.000 80 36 0 6 85 7 86 1.46e-26 100
MS.gene35048.t1 MTR_7g105260 43.038 79 45 0 7 85 32 110 1.28e-18 79.0
MS.gene35048.t1 MTR_3g025880 40.476 84 50 0 1 84 38 121 5.83e-18 81.6
MS.gene35048.t1 MTR_3g080130 47.368 76 40 0 8 83 37 112 3.32e-17 75.1
MS.gene35048.t1 MTR_3g075500 45.455 88 46 1 4 89 203 290 8.98e-17 77.0
MS.gene35048.t1 MTR_1g045510 41.975 81 47 0 4 84 201 281 1.34e-16 76.6
MS.gene35048.t1 MTR_7g099940 46.250 80 43 0 8 87 36 115 1.48e-16 73.6
MS.gene35048.t1 MTR_1g092860 44.156 77 43 0 8 84 193 269 2.37e-16 75.9
MS.gene35048.t1 MTR_4g109540 36.905 84 53 0 1 84 36 119 3.82e-16 75.1
MS.gene35048.t1 MTR_7g013730 40.789 76 45 0 8 83 41 116 4.58e-16 74.7
MS.gene35048.t1 MTR_3g084040 41.975 81 46 1 8 88 37 116 2.37e-15 70.5
MS.gene35048.t1 MTR_4g035310 46.479 71 37 1 3 72 12 82 3.95e-15 72.4
MS.gene35048.t1 MTR_4g035310 46.479 71 37 1 3 72 12 82 4.71e-15 72.0
MS.gene35048.t1 MTR_2g096560 40.244 82 49 0 8 89 8 89 6.04e-15 67.8
MS.gene35048.t1 MTR_7g117495 45.833 72 35 1 3 70 14 85 1.23e-14 70.9
MS.gene35048.t1 MTR_7g117495 45.833 72 35 1 3 70 14 85 1.50e-14 70.5
MS.gene35048.t1 MTR_8g024520 40.741 81 41 2 2 76 14 93 1.83e-14 70.9
MS.gene35048.t1 MTR_8g024520 40.741 81 41 2 2 76 14 93 1.87e-14 70.9
MS.gene35048.t1 MTR_6g477870 41.026 78 46 0 8 85 96 173 1.92e-14 68.9
MS.gene35048.t1 MTR_4g109540 39.189 74 45 0 11 84 54 127 4.64e-14 69.3
MS.gene35048.t1 MTR_1g064230 46.053 76 41 0 8 83 197 272 7.61e-14 68.9
MS.gene35048.t1 MTR_4g104470 43.590 78 44 0 7 84 216 293 1.43e-13 68.6
MS.gene35048.t1 MTR_4g104470 38.272 81 50 0 5 85 120 200 6.68e-13 66.6
MS.gene35048.t1 MTR_3g029580 32.353 102 64 1 8 109 33 129 1.50e-13 65.5
MS.gene35048.t1 MTR_2g026180 35.556 90 51 2 8 97 5 87 1.79e-13 68.9
MS.gene35048.t1 MTR_2g026180 36.709 79 49 1 8 86 107 184 8.26e-13 67.0
MS.gene35048.t1 MTR_5g097580 31.250 112 71 3 8 116 7 115 2.25e-13 68.6
MS.gene35048.t1 MTR_5g097580 36.585 82 51 1 8 89 107 187 1.46e-12 66.2
MS.gene35048.t1 MTR_1g073690 39.241 79 47 1 8 86 113 190 2.74e-13 68.2
MS.gene35048.t1 MTR_1g073690 33.333 81 52 1 3 83 2 80 7.92e-12 63.9
MS.gene35048.t1 MTR_5g097580 31.250 112 71 3 8 116 7 115 2.80e-13 68.2
MS.gene35048.t1 MTR_5g097580 36.585 82 51 1 8 89 107 187 1.42e-12 66.2
MS.gene35048.t1 MTR_6g004810 31.325 83 57 0 8 90 134 216 2.82e-13 68.2
MS.gene35048.t1 MTR_3g055990 31.624 117 74 3 3 116 2 115 3.82e-13 67.8
MS.gene35048.t1 MTR_3g055990 35.366 82 52 1 8 89 107 187 3.20e-11 62.4
MS.gene35048.t1 MTR_3g055990 31.624 117 74 3 3 116 2 115 3.83e-13 67.8
MS.gene35048.t1 MTR_3g055990 35.366 82 52 1 8 89 107 187 3.63e-11 62.0
MS.gene35048.t1 MTR_2g026180 36.709 79 49 1 8 86 37 114 4.61e-13 67.4
MS.gene35048.t1 MTR_3g116130 48.276 58 30 0 3 60 25 82 8.84e-13 65.9
MS.gene35048.t1 MTR_3g082650 35.000 80 52 0 8 87 107 186 9.44e-13 66.2
MS.gene35048.t1 MTR_4g081520 42.353 85 43 2 4 85 48 129 1.24e-12 63.2
MS.gene35048.t1 MTR_7g106440 37.662 77 48 0 7 83 212 288 1.48e-12 65.5
MS.gene35048.t1 MTR_7g107580 39.189 74 45 0 9 82 350 423 1.76e-12 65.9
MS.gene35048.t1 MTR_7g107580 39.189 74 45 0 9 82 351 424 1.93e-12 65.9
MS.gene35048.t1 MTR_3g075500 44.776 67 37 0 4 70 203 269 1.96e-12 65.1
MS.gene35048.t1 MTR_5g064120 42.029 69 39 1 8 76 17 84 2.47e-12 64.7
MS.gene35048.t1 MTR_3g116130 48.276 58 30 0 3 60 25 82 3.30e-12 65.1
MS.gene35048.t1 MTR_3g103420 36.364 88 53 2 8 94 118 203 3.90e-12 64.7
MS.gene35048.t1 MTR_4g117350 36.471 85 52 1 8 92 72 154 7.16e-12 61.6
MS.gene35048.t1 MTR_3g084040 35.616 73 41 1 8 80 37 103 7.51e-12 60.1
MS.gene35048.t1 MTR_4g063560 38.356 73 45 0 8 80 34 106 8.67e-12 60.1
MS.gene35048.t1 MTR_5g044970 30.645 124 73 3 9 122 12 132 1.44e-11 60.8
MS.gene35048.t1 MTR_3g065190 44.737 76 41 1 9 83 114 189 1.60e-11 62.8
MS.gene35048.t1 MTR_8g074790 40.789 76 45 0 9 84 64 139 2.89e-11 61.6
MS.gene35048.t1 MTR_1g056520 33.333 90 60 0 7 96 207 296 4.72e-11 61.2
MS.gene35048.t1 MTR_2g013130 32.222 90 61 0 8 97 284 373 6.52e-11 61.2
MS.gene35048.t1 MTR_2g013130 32.222 90 61 0 8 97 299 388 8.03e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35048.t1 AT3G26420 53.755 253 62 7 1 208 1 243 9.07e-69 211
MS.gene35048.t1 AT4G39260 60.976 82 32 0 4 85 3 84 1.46e-30 108
MS.gene35048.t1 AT4G39260 61.728 81 31 0 4 84 3 83 4.42e-29 107
MS.gene35048.t1 AT2G21660 60.759 79 31 0 6 84 7 85 1.10e-28 105
MS.gene35048.t1 AT1G60650 41.221 131 68 3 6 136 11 132 1.69e-28 108
MS.gene35048.t1 AT1G60650 41.221 131 68 3 6 136 11 132 1.69e-28 108
MS.gene35048.t1 AT2G21660 60.759 79 31 0 6 84 7 85 1.73e-28 105
MS.gene35048.t1 AT5G04280 38.953 172 87 4 1 159 1 167 8.62e-26 102
MS.gene35048.t1 AT4G39260 60.606 66 26 0 4 69 3 68 2.34e-22 87.8
MS.gene35048.t1 AT4G13850 46.429 84 45 0 8 91 36 119 7.36e-19 79.7
MS.gene35048.t1 AT4G13850 46.429 84 45 0 8 91 36 119 9.14e-19 79.7
MS.gene35048.t1 AT4G13850 49.351 77 39 0 8 84 36 112 9.77e-19 79.0
MS.gene35048.t1 AT4G13850 46.429 84 45 0 8 91 36 119 1.03e-18 79.7
MS.gene35048.t1 AT3G23830 43.023 86 49 0 8 93 36 121 5.12e-18 77.4
MS.gene35048.t1 AT3G23830 43.023 86 49 0 8 93 36 121 5.12e-18 77.4
MS.gene35048.t1 AT2G37220 48.052 77 40 0 8 84 205 281 9.86e-18 79.7
MS.gene35048.t1 AT2G37220 38.202 89 53 1 6 94 90 176 3.46e-12 64.3
MS.gene35048.t1 AT3G53460 46.753 77 41 0 8 84 258 334 6.04e-17 78.2
MS.gene35048.t1 AT3G53460 46.753 77 41 0 8 84 258 334 6.04e-17 78.2
MS.gene35048.t1 AT3G53460 46.753 77 41 0 8 84 258 334 6.04e-17 78.2
MS.gene35048.t1 AT5G06210 42.500 80 46 0 8 87 35 114 6.34e-17 74.7
MS.gene35048.t1 AT5G61030 43.421 76 43 0 8 83 41 116 7.58e-17 77.8
MS.gene35048.t1 AT5G61030 43.421 76 43 0 8 83 41 116 7.58e-17 77.8
MS.gene35048.t1 AT3G53460 46.753 77 41 0 8 84 279 355 9.91e-17 77.8
MS.gene35048.t1 AT1G18630 50.000 76 38 0 8 83 37 112 1.19e-16 74.3
MS.gene35048.t1 AT1G33470 47.945 73 37 1 1 72 1 73 4.82e-16 74.3
MS.gene35048.t1 AT1G33470 47.945 73 37 1 1 72 1 73 5.95e-16 74.3
MS.gene35048.t1 AT1G33470 47.945 73 37 1 1 72 1 73 5.99e-16 74.3
MS.gene35048.t1 AT2G46780 46.667 75 39 1 2 76 17 90 1.84e-15 73.9
MS.gene35048.t1 AT2G46780 46.667 75 39 1 2 76 17 90 1.84e-15 73.9
MS.gene35048.t1 AT1G22910 46.575 73 38 1 1 72 7 79 1.94e-15 73.2
MS.gene35048.t1 AT4G24770 43.210 81 46 0 4 84 241 321 2.07e-15 73.9
MS.gene35048.t1 AT4G24770 38.272 81 50 0 5 85 148 228 1.60e-12 65.9
MS.gene35048.t1 AT4G24770 43.210 81 46 0 4 84 241 321 2.07e-15 73.9
MS.gene35048.t1 AT4G24770 38.272 81 50 0 5 85 148 228 1.60e-12 65.9
MS.gene35048.t1 AT5G04280 40.299 134 62 4 39 159 1 129 2.45e-15 73.2
MS.gene35048.t1 AT5G04280 40.299 134 62 4 39 159 1 129 2.45e-15 73.2
MS.gene35048.t1 AT5G04280 40.299 134 62 4 39 159 1 129 2.45e-15 73.2
MS.gene35048.t1 AT5G04280 40.299 134 62 4 39 159 1 129 2.45e-15 73.2
MS.gene35048.t1 AT1G22910 46.575 73 38 1 1 72 7 79 2.65e-15 72.4
MS.gene35048.t1 AT5G47320 35.922 103 64 1 8 108 32 134 3.81e-15 71.6
MS.gene35048.t1 AT5G59865 43.038 79 45 0 8 86 27 105 4.74e-15 68.9
MS.gene35048.t1 AT1G60000 41.667 84 49 0 1 84 171 254 5.10e-15 72.0
MS.gene35048.t1 AT2G21690 46.377 69 37 0 1 69 1 69 5.51e-15 68.9
MS.gene35048.t1 AT1G73530 35.514 107 61 2 8 111 78 179 5.84e-15 70.5
MS.gene35048.t1 AT1G22910 46.575 73 38 1 1 72 7 79 2.01e-14 71.2
MS.gene35048.t1 AT1G60650 36.538 104 57 3 33 136 1 95 2.12e-14 70.1
MS.gene35048.t1 AT2G37510 50.000 74 37 0 8 81 35 108 2.48e-14 67.8
MS.gene35048.t1 AT3G20930 41.558 77 45 0 8 84 283 359 3.30e-14 70.9
MS.gene35048.t1 AT1G74230 43.421 76 42 1 8 83 35 109 6.05e-14 69.3
MS.gene35048.t1 AT1G03457 41.096 73 43 0 9 81 227 299 7.22e-14 69.3
MS.gene35048.t1 AT3G13224 32.143 84 57 0 8 91 110 193 8.56e-14 69.3
MS.gene35048.t1 AT1G60650 38.776 98 51 3 39 136 1 89 1.01e-13 68.2
MS.gene35048.t1 AT3G08000 44.737 76 42 0 8 83 42 117 1.05e-13 66.2
MS.gene35048.t1 AT4G14300 37.805 82 49 1 3 84 2 81 1.06e-13 69.3
MS.gene35048.t1 AT4G14300 36.709 79 49 1 8 86 111 188 4.88e-12 64.7
MS.gene35048.t1 AT4G14300 37.805 82 49 1 3 84 2 81 1.06e-13 69.3
MS.gene35048.t1 AT4G14300 36.709 79 49 1 8 86 111 188 4.88e-12 64.7
MS.gene35048.t1 AT4G26650 32.456 114 70 2 2 115 10 116 1.18e-13 69.3
MS.gene35048.t1 AT4G26650 32.456 114 70 2 2 115 7 113 1.29e-13 69.3
MS.gene35048.t1 AT1G78260 43.478 69 38 1 8 76 18 85 1.41e-13 68.2
MS.gene35048.t1 AT3G07810 34.615 78 49 1 8 85 7 82 1.46e-13 69.3
MS.gene35048.t1 AT3G07810 37.805 82 50 1 8 89 109 189 1.22e-12 66.6
MS.gene35048.t1 AT3G07810 34.615 78 49 1 8 85 7 82 1.50e-13 68.9
MS.gene35048.t1 AT3G07810 37.805 82 50 1 8 89 109 189 1.35e-12 66.2
MS.gene35048.t1 AT3G13224 32.143 84 57 0 8 91 101 184 1.86e-13 68.6
MS.gene35048.t1 AT1G03457 41.096 73 43 0 9 81 341 413 2.20e-13 68.6
MS.gene35048.t1 AT4G13860 42.466 73 42 0 8 80 4 76 2.26e-13 63.5
MS.gene35048.t1 AT1G03457 41.096 73 43 0 9 81 332 404 2.28e-13 68.6
MS.gene35048.t1 AT1G76460 50.943 53 26 0 8 60 25 77 2.50e-13 67.8
MS.gene35048.t1 AT5G47620 37.179 78 47 1 8 85 7 82 3.41e-13 68.2
MS.gene35048.t1 AT5G47620 37.179 78 47 1 8 85 7 82 3.41e-13 68.2
MS.gene35048.t1 AT5G47620 37.179 78 47 1 8 85 7 82 3.41e-13 68.2
MS.gene35048.t1 AT1G22330 39.189 74 44 1 3 76 13 85 7.03e-13 66.2
MS.gene35048.t1 AT1G78260 43.478 69 38 1 8 76 18 85 7.62e-13 66.2
MS.gene35048.t1 AT1G20880 45.455 66 35 1 8 72 25 90 8.48e-13 65.9
MS.gene35048.t1 AT1G20880 45.455 66 35 1 8 72 25 90 8.48e-13 65.9
MS.gene35048.t1 AT1G20880 45.455 66 35 1 8 72 25 90 8.48e-13 65.9
MS.gene35048.t1 AT5G55550 30.952 126 79 3 8 133 7 124 9.55e-13 66.6
MS.gene35048.t1 AT5G55550 30.952 126 79 3 8 133 7 124 9.55e-13 66.6
MS.gene35048.t1 AT5G55550 30.952 126 79 3 8 133 7 124 9.55e-13 66.6
MS.gene35048.t1 AT5G55550 30.952 126 79 3 8 133 7 124 9.55e-13 66.6
MS.gene35048.t1 AT5G55550 30.952 126 79 3 8 133 7 124 9.55e-13 66.6
MS.gene35048.t1 AT5G55550 32.075 106 65 2 8 113 7 105 1.15e-12 66.6
MS.gene35048.t1 AT5G55550 32.075 106 65 2 8 113 7 105 1.15e-12 66.6
MS.gene35048.t1 AT5G55550 32.075 106 65 2 8 113 7 105 1.15e-12 66.6
MS.gene35048.t1 AT5G55550 32.075 106 65 2 8 113 7 105 1.15e-12 66.6
MS.gene35048.t1 AT5G40490 36.145 83 53 0 8 90 131 213 1.29e-12 66.2
MS.gene35048.t1 AT1G58470 36.364 88 55 1 8 95 121 207 1.79e-12 65.9
MS.gene35048.t1 AT1G58470 36.364 88 55 1 8 95 121 207 1.79e-12 65.9
MS.gene35048.t1 AT5G50250 41.026 78 46 0 7 84 207 284 3.43e-12 64.3
MS.gene35048.t1 AT3G20930 39.024 82 45 1 8 84 283 364 3.68e-12 64.7
MS.gene35048.t1 AT3G54770 41.667 72 38 1 3 70 13 84 3.86e-12 63.9
MS.gene35048.t1 AT3G54770 41.667 72 38 1 3 70 13 84 4.42e-12 63.9
MS.gene35048.t1 AT5G47620 40.000 65 39 0 8 72 7 71 8.17e-12 63.9
MS.gene35048.t1 AT5G40490 37.931 87 46 2 8 90 114 196 8.96e-12 63.9
MS.gene35048.t1 AT5G53720 41.026 78 45 1 6 83 8 84 1.28e-11 59.3
MS.gene35048.t1 AT5G54580 40.449 89 47 2 4 89 53 138 1.54e-11 60.5
MS.gene35048.t1 AT4G03110 41.096 73 43 0 9 81 351 423 2.44e-11 62.4
MS.gene35048.t1 AT3G46020 34.524 84 55 0 1 84 1 84 3.04e-11 58.5
MS.gene35048.t1 AT5G55550 38.028 71 42 1 8 78 7 75 4.55e-11 62.0
MS.gene35048.t1 AT2G33410 34.177 79 51 1 8 86 111 188 5.46e-11 61.6
MS.gene35048.t1 AT2G33410 32.927 82 53 1 3 84 2 81 7.18e-11 61.2
MS.gene35048.t1 AT5G55550 38.028 71 42 1 8 78 7 75 5.55e-11 61.6

Find 60 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGGACGCTCTCGTGGTTT+TGG 0.216628 4.4:-20528253 MS.gene35048:CDS
GGTGGTAGTCGTGGAGATTC+TGG 0.273094 4.4:-20527845 MS.gene35048:CDS
AGGGATACATTTGAAAAGTT+TGG 0.280841 4.4:-20528607 MS.gene35048:CDS
GTTCTTCTGGCGGTCGCAGC+AGG 0.318323 4.4:-20527871 MS.gene35048:CDS
AGTGGTGGTGGTGGTCACTA+TGG 0.339869 4.4:-20527914 MS.gene35048:CDS
AGAAATGGAGACCGTTCTTC+TGG 0.343709 4.4:-20527884 MS.gene35048:CDS
AGGGAGTGCCCTAGTGGTGA+AGG 0.394081 4.4:-20527989 MS.gene35048:CDS
CCATATGAACGACGTGGATC+TGG 0.395444 4.4:-20527785 MS.gene35048:CDS
GAACGGTCTCCATTTCTATC+TGG 0.397184 4.4:+20527890 None:intergenic
CAACATCCGATAGAAAGTTA+AGG 0.426777 4.4:-20528627 MS.gene35048:CDS
GTGGTTGTTGACAAATTCTC+TGG 0.436058 4.4:-20528271 MS.gene35048:CDS
GAAAGTAGATATGGTGGAAG+TGG 0.439807 4.4:-20527932 MS.gene35048:CDS
CCAGATCCACGTCGTTCATA+TGG 0.442836 4.4:+20527785 None:intergenic
CACTATGGTCCAGATAGAAA+TGG 0.454742 4.4:-20527899 MS.gene35048:CDS
CGAGATTACCGAGGTGGTCG+AGG 0.461040 4.4:-20528061 MS.gene35048:CDS
GGTGGTAGAGAAAGTAGATA+TGG 0.473535 4.4:-20527941 MS.gene35048:CDS
CTCTATCAACAGTAATAGTT+CGG 0.474868 4.4:+20528158 None:intergenic
ATGAATGGGATAGATTTAGA+TGG 0.485420 4.4:-20528181 MS.gene35048:CDS
AGATATGGTGGAAGTGGTGG+TGG 0.486452 4.4:-20527926 MS.gene35048:CDS
AACATCCGATAGAAAGTTAA+GGG 0.491433 4.4:-20528626 MS.gene35048:CDS
CAAGGATCCTCTAGAGATGA+TGG 0.492928 4.4:-20528124 MS.gene35048:CDS
TCTGGCGGTCGCAGCAGGGA+TGG 0.500920 4.4:-20527866 MS.gene35048:CDS
CGCGATCGCGACCACGCTCT+CGG 0.506416 4.4:+20528092 None:intergenic
TATGGTGGAAGTGGTGGTGG+TGG 0.507336 4.4:-20527923 MS.gene35048:CDS
GGTAGAGAAAGTAGATATGG+TGG 0.507553 4.4:-20527938 MS.gene35048:CDS
AAATTCTCTGGACGCTCTCG+TGG 0.522134 4.4:-20528259 MS.gene35048:CDS
GATGCTATTGATGCTATGAA+TGG 0.526053 4.4:-20528196 MS.gene35048:CDS
TATCGGATGTTGACCAAGCA+AGG 0.526702 4.4:+20528638 None:intergenic
TGTATCCCTTAACTTTCTAT+CGG 0.530467 4.4:+20528621 None:intergenic
TGCTTCAATTGTGGTAAACC+TGG 0.534286 4.4:-20528022 MS.gene35048:CDS
GGGAGTGCCCTAGTGGTGAA+GGG 0.544084 4.4:-20527988 MS.gene35048:CDS
TTCTTCTGGCGGTCGCAGCA+GGG 0.544328 4.4:-20527870 MS.gene35048:CDS
TGGCAAGCTTACAGAGGCCA+AGG 0.548700 4.4:-20528587 MS.gene35048:intron
TGCTGCGACCGCCAGAAGAA+CGG 0.550061 4.4:+20527873 None:intergenic
TATCGTGATCGTGATCGTGC+TGG 0.552506 4.4:-20527809 MS.gene35048:CDS
GGCGGTCGCAGCAGGGATGG+TGG 0.563764 4.4:-20527863 MS.gene35048:CDS
AGCAGGGATGGTGGTAGTCG+TGG 0.574692 4.4:-20527854 MS.gene35048:CDS
CACTCCCTAGCAAAATGACC+AGG 0.578775 4.4:+20528004 None:intergenic
GGTGGTCGAGGATCTAATGA+TGG 0.580077 4.4:-20528049 MS.gene35048:CDS
GGAGTGCCCTAGTGGTGAAG+GGG 0.582980 4.4:-20527987 MS.gene35048:CDS
AGTAGATATGGTGGAAGTGG+TGG 0.583787 4.4:-20527929 MS.gene35048:CDS
GCAGTTTAAAATGTCGGACG+AGG 0.587416 4.4:-20528686 None:intergenic
AATGGAGACCGTTCTTCTGG+CGG 0.587666 4.4:-20527881 MS.gene35048:CDS
ATGCTATTGATGCTATGAAT+GGG 0.592487 4.4:-20528195 MS.gene35048:CDS
TTTGCTAGGGAGTGCCCTAG+TGG 0.594989 4.4:-20527995 MS.gene35048:CDS
GAACGACGTGGATCTGGAGG+CGG 0.604365 4.4:-20527779 MS.gene35048:CDS
ATATGATGAAAAGAAAGCAA+TGG 0.616878 4.4:-20528221 MS.gene35048:CDS
GAGTGCCCTAGTGGTGAAGG+GGG 0.631815 4.4:-20527986 MS.gene35048:CDS
GGTGATCGCTACCGAGAGCG+TGG 0.633883 4.4:-20528103 MS.gene35048:CDS
TCTCTAGAGGATCCTTGTTG+AGG 0.640001 4.4:+20528130 None:intergenic
TATGAACGACGTGGATCTGG+AGG 0.640937 4.4:-20527782 MS.gene35048:CDS
GATAGAGCTCAGCCTCAACA+AGG 0.641644 4.4:-20528142 MS.gene35048:CDS
GCGATCACCATCATCTCTAG+AGG 0.642537 4.4:+20528117 None:intergenic
CATTAGATCCTCGACCACCT+CGG 0.645981 4.4:+20528053 None:intergenic
GATGGAGGATGCTTCAATTG+TGG 0.663732 4.4:-20528031 MS.gene35048:CDS
AAAGTTTGGCAAGCTTACAG+AGG 0.664669 4.4:-20528593 MS.gene35048:CDS
GGTCGAGGATCTAATGATGG+AGG 0.667781 4.4:-20528046 MS.gene35048:CDS
CGTGATCGAGATTACCGAGG+TGG 0.705905 4.4:-20528067 MS.gene35048:CDS
GAACGTGATCGAGATTACCG+AGG 0.730869 4.4:-20528070 MS.gene35048:CDS
GCTGGACCATATGAACGACG+TGG 0.755617 4.4:-20527791 MS.gene35048:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATGTAATATGATAAAAAA+TGG - chr4.4:20528072-20528091 MS.gene35048:CDS 15.0%
!! TTACTTGATTTACTAATAAA+AGG + chr4.4:20528035-20528054 None:intergenic 15.0%
!!! TTTCATTTTATTTTGTGAAA+AGG - chr4.4:20528152-20528171 MS.gene35048:CDS 15.0%
!! TCTGAACATTATATTTGATT+TGG - chr4.4:20528105-20528124 MS.gene35048:CDS 20.0%
!!! ATTATATTTGATTTGGAGAA+TGG - chr4.4:20528112-20528131 MS.gene35048:CDS 20.0%
!!! CTTTTGTTCATTTTTATTTG+AGG - chr4.4:20527985-20528004 MS.gene35048:CDS 20.0%
! ATATGATGAAAAGAAAGCAA+TGG - chr4.4:20528224-20528243 MS.gene35048:CDS 25.0%
!!! AAAGATAAAGTTTGTTGTCT+AGG + chr4.4:20527969-20527988 None:intergenic 25.0%
!!! CATTTTATTTTGTGAAAAGG+TGG - chr4.4:20528155-20528174 MS.gene35048:CDS 25.0%
AACATCCGATAGAAAGTTAA+GGG - chr4.4:20527819-20527838 MS.gene35048:CDS 30.0%
AGGGATACATTTGAAAAGTT+TGG - chr4.4:20527838-20527857 MS.gene35048:CDS 30.0%
ATGAATGGGATAGATTTAGA+TGG - chr4.4:20528264-20528283 MS.gene35048:CDS 30.0%
ATGCTATTGATGCTATGAAT+GGG - chr4.4:20528250-20528269 MS.gene35048:CDS 30.0%
CTCTATCAACAGTAATAGTT+CGG + chr4.4:20528290-20528309 None:intergenic 30.0%
TGTATCCCTTAACTTTCTAT+CGG + chr4.4:20527827-20527846 None:intergenic 30.0%
! ATTTGAGATATTGATCACCT+TGG + chr4.4:20527878-20527897 None:intergenic 30.0%
CAACATCCGATAGAAAGTTA+AGG - chr4.4:20527818-20527837 MS.gene35048:CDS 35.0%
GATGCTATTGATGCTATGAA+TGG - chr4.4:20528249-20528268 MS.gene35048:CDS 35.0%
TACAGCATAAACCAACTTCT+AGG + chr4.4:20527933-20527952 None:intergenic 35.0%
TCATGTTTGTACCTAGAAGT+TGG - chr4.4:20527919-20527938 MS.gene35048:CDS 35.0%
!! GATGAGTATCGTTGTTTTGT+TGG - chr4.4:20527781-20527800 MS.gene35048:CDS 35.0%
!! TAAACCTGGTCATTTTGCTA+GGG - chr4.4:20528437-20528456 MS.gene35048:intron 35.0%
AAAGTTTGGCAAGCTTACAG+AGG - chr4.4:20527852-20527871 MS.gene35048:CDS 40.0%
CACTATGGTCCAGATAGAAA+TGG - chr4.4:20528546-20528565 MS.gene35048:intron 40.0%
GGTAGAGAAAGTAGATATGG+TGG - chr4.4:20528507-20528526 MS.gene35048:intron 40.0%
GGTGGTAGAGAAAGTAGATA+TGG - chr4.4:20528504-20528523 MS.gene35048:intron 40.0%
GTGGTTGTTGACAAATTCTC+TGG - chr4.4:20528174-20528193 MS.gene35048:CDS 40.0%
TGCTTCAATTGTGGTAAACC+TGG - chr4.4:20528423-20528442 MS.gene35048:intron 40.0%
! GAAAGTAGATATGGTGGAAG+TGG - chr4.4:20528513-20528532 MS.gene35048:intron 40.0%
!! GAGTATCGTTGTTTTGTTGG+TGG - chr4.4:20527784-20527803 MS.gene35048:CDS 40.0%
!! GTAAACCTGGTCATTTTGCT+AGG - chr4.4:20528436-20528455 MS.gene35048:intron 40.0%
AGAAATGGAGACCGTTCTTC+TGG - chr4.4:20528561-20528580 MS.gene35048:intron 45.0%
GAACGGTCTCCATTTCTATC+TGG + chr4.4:20528558-20528577 None:intergenic 45.0%
GATGGAGGATGCTTCAATTG+TGG - chr4.4:20528414-20528433 MS.gene35048:intron 45.0%
TATCGGATGTTGACCAAGCA+AGG + chr4.4:20527810-20527829 None:intergenic 45.0%
! AGTAGATATGGTGGAAGTGG+TGG - chr4.4:20528516-20528535 MS.gene35048:intron 45.0%
! CAAGGATCCTCTAGAGATGA+TGG - chr4.4:20528321-20528340 MS.gene35048:intron 45.0%
! TCTCTAGAGGATCCTTGTTG+AGG + chr4.4:20528318-20528337 None:intergenic 45.0%
AAATTCTCTGGACGCTCTCG+TGG - chr4.4:20528186-20528205 MS.gene35048:CDS 50.0%
AATGGAGACCGTTCTTCTGG+CGG - chr4.4:20528564-20528583 MS.gene35048:intron 50.0%
CACTCCCTAGCAAAATGACC+AGG + chr4.4:20528444-20528463 None:intergenic 50.0%
CATTAGATCCTCGACCACCT+CGG + chr4.4:20528395-20528414 None:intergenic 50.0%
CCAGATCCACGTCGTTCATA+TGG + chr4.4:20528663-20528682 None:intergenic 50.0%
CCATATGAACGACGTGGATC+TGG - chr4.4:20528660-20528679 MS.gene35048:CDS 50.0%
GAACGTGATCGAGATTACCG+AGG - chr4.4:20528375-20528394 MS.gene35048:intron 50.0%
GATAGAGCTCAGCCTCAACA+AGG - chr4.4:20528303-20528322 MS.gene35048:intron 50.0%
GCGATCACCATCATCTCTAG+AGG + chr4.4:20528331-20528350 None:intergenic 50.0%
GGTCGAGGATCTAATGATGG+AGG - chr4.4:20528399-20528418 MS.gene35048:intron 50.0%
GGTGGTCGAGGATCTAATGA+TGG - chr4.4:20528396-20528415 MS.gene35048:intron 50.0%
TATCGTGATCGTGATCGTGC+TGG - chr4.4:20528636-20528655 MS.gene35048:CDS 50.0%
TATGAACGACGTGGATCTGG+AGG - chr4.4:20528663-20528682 MS.gene35048:CDS 50.0%
! AGATATGGTGGAAGTGGTGG+TGG - chr4.4:20528519-20528538 MS.gene35048:intron 50.0%
!!! GTTTTGTTGGTGGCCTTGCT+TGG - chr4.4:20527794-20527813 MS.gene35048:CDS 50.0%
CGTGATCGAGATTACCGAGG+TGG - chr4.4:20528378-20528397 MS.gene35048:intron 55.0%
CTAGTGGTGAAGGGGGAAGA+GGG - chr4.4:20528466-20528485 MS.gene35048:intron 55.0%
CTCTTCCCCCTTCACCACTA+GGG + chr4.4:20528467-20528486 None:intergenic 55.0%
GCTGGACCATATGAACGACG+TGG - chr4.4:20528654-20528673 MS.gene35048:CDS 55.0%
GGTGGTAGTCGTGGAGATTC+TGG - chr4.4:20528600-20528619 MS.gene35048:CDS 55.0%
TAGTGGTGAAGGGGGAAGAG+GGG - chr4.4:20528467-20528486 MS.gene35048:intron 55.0%
TGGCAAGCTTACAGAGGCCA+AGG - chr4.4:20527858-20527877 MS.gene35048:CDS 55.0%
! TCTGGACGCTCTCGTGGTTT+TGG - chr4.4:20528192-20528211 MS.gene35048:CDS 55.0%
!! AGTGGTGGTGGTGGTCACTA+TGG - chr4.4:20528531-20528550 MS.gene35048:intron 55.0%
!! TATGGTGGAAGTGGTGGTGG+TGG - chr4.4:20528522-20528541 MS.gene35048:intron 55.0%
!! TTTGCTAGGGAGTGCCCTAG+TGG - chr4.4:20528450-20528469 MS.gene35048:intron 55.0%
AGTGGTGAAGGGGGAAGAGG+GGG - chr4.4:20528468-20528487 MS.gene35048:intron 60.0%
CCTAGTGGTGAAGGGGGAAG+AGG - chr4.4:20528465-20528484 MS.gene35048:intron 60.0%
CCTCTTCCCCCTTCACCACT+AGG + chr4.4:20528468-20528487 None:intergenic 60.0%
CGAGATTACCGAGGTGGTCG+AGG - chr4.4:20528384-20528403 MS.gene35048:intron 60.0%
GAACGACGTGGATCTGGAGG+CGG - chr4.4:20528666-20528685 MS.gene35048:CDS 60.0%
GAGTGCCCTAGTGGTGAAGG+GGG - chr4.4:20528459-20528478 MS.gene35048:intron 60.0%
TGCTGCGACCGCCAGAAGAA+CGG + chr4.4:20528575-20528594 None:intergenic 60.0%
TTCTTCTGGCGGTCGCAGCA+GGG - chr4.4:20528575-20528594 MS.gene35048:intron 60.0%
! AGCAGGGATGGTGGTAGTCG+TGG - chr4.4:20528591-20528610 MS.gene35048:CDS 60.0%
! GGAGTGCCCTAGTGGTGAAG+GGG - chr4.4:20528458-20528477 MS.gene35048:intron 60.0%
!! AGGGAGTGCCCTAGTGGTGA+AGG - chr4.4:20528456-20528475 MS.gene35048:intron 60.0%
!! GGGAGTGCCCTAGTGGTGAA+GGG - chr4.4:20528457-20528476 MS.gene35048:intron 60.0%
AGAGGGGGAGGGGGAAGGTA+TGG - chr4.4:20528483-20528502 MS.gene35048:intron 65.0%
GGTGATCGCTACCGAGAGCG+TGG - chr4.4:20528342-20528361 MS.gene35048:intron 65.0%
GTGAAGGGGGAAGAGGGGGA+GGG - chr4.4:20528472-20528491 MS.gene35048:intron 65.0%
GTTCTTCTGGCGGTCGCAGC+AGG - chr4.4:20528574-20528593 MS.gene35048:intron 65.0%
TGAAGGGGGAAGAGGGGGAG+GGG - chr4.4:20528473-20528492 MS.gene35048:intron 65.0%
CGCGATCGCGACCACGCTCT+CGG + chr4.4:20528356-20528375 None:intergenic 70.0%
GAAGGGGGAAGAGGGGGAGG+GGG - chr4.4:20528474-20528493 MS.gene35048:intron 70.0%
GGTGAAGGGGGAAGAGGGGG+AGG - chr4.4:20528471-20528490 MS.gene35048:intron 70.0%
TCTGGCGGTCGCAGCAGGGA+TGG - chr4.4:20528579-20528598 MS.gene35048:intron 70.0%
! GGGGGAGGGGGAAGGTATGG+TGG - chr4.4:20528486-20528505 MS.gene35048:intron 70.0%
GGCGGTCGCAGCAGGGATGG+TGG - chr4.4:20528582-20528601 MS.gene35048:intron 75.0%
GGGGAAGAGGGGGAGGGGGA+AGG - chr4.4:20528478-20528497 MS.gene35048:intron 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 20527769 20528698 20527769 ID=MS.gene35048
chr4.4 mRNA 20527769 20528698 20527769 ID=MS.gene35048.t1;Parent=MS.gene35048
chr4.4 exon 20528588 20528698 20528588 ID=MS.gene35048.t1.exon1;Parent=MS.gene35048.t1
chr4.4 CDS 20528588 20528698 20528588 ID=cds.MS.gene35048.t1;Parent=MS.gene35048.t1
chr4.4 exon 20527769 20528293 20527769 ID=MS.gene35048.t1.exon2;Parent=MS.gene35048.t1
chr4.4 CDS 20527769 20528293 20527769 ID=cds.MS.gene35048.t1;Parent=MS.gene35048.t1
Gene Sequence

>MS.gene35048

ATGTCGGACGAGGATGAGTATCGTTGTTTTGTTGGTGGCCTTGCTTGGTCAACATCCGATAGAAAGTTAAGGGATACATTTGAAAAGTTTGGCAAGCTTACAGAGGCCAAGGTGATCAATATCTCAAATTAATGTTGTCATTATCTTATTTCATGTTTGTACCTAGAAGTTGGTTTATGCTGTAATTTTAGCTTACACCTAGACAACAAACTTTATCTTTTGTTCATTTTTATTTGAGGAGAAAATTTGCAATCTATACTGAACCTTTTATTAGTAAATCAAGTAAACAAGATAAAAAGAAATCAATGTAATATGATAAAAAATGGATAACTGTTTTCTGAACATTATATTTGATTTGGAGAATGGAGATTGTTAATGTTCTCTTTCATTTTATTTTGTGAAAAGGTGGTTGTTGACAAATTCTCTGGACGCTCTCGTGGTTTTGGATTTGTCACATATGATGAAAAGAAAGCAATGGAAGATGCTATTGATGCTATGAATGGGATAGATTTAGATGGCCGAACTATTACTGTTGATAGAGCTCAGCCTCAACAAGGATCCTCTAGAGATGATGGTGATCGCTACCGAGAGCGTGGTCGCGATCGCGAACGTGATCGAGATTACCGAGGTGGTCGAGGATCTAATGATGGAGGATGCTTCAATTGTGGTAAACCTGGTCATTTTGCTAGGGAGTGCCCTAGTGGTGAAGGGGGAAGAGGGGGAGGGGGAAGGTATGGTGGTAGAGAAAGTAGATATGGTGGAAGTGGTGGTGGTGGTCACTATGGTCCAGATAGAAATGGAGACCGTTCTTCTGGCGGTCGCAGCAGGGATGGTGGTAGTCGTGGAGATTCTGGAAGTGATCGATACTATCGTGATCGTGATCGTGCTGGACCATATGAACGACGTGGATCTGGAGGCGGCTATCGTTGA

Protein sequence

>MS.gene35048.t1

MSDEDEYRCFVGGLAWSTSDRKLRDTFEKFGKLTEAKVVVDKFSGRSRGFGFVTYDEKKAMEDAIDAMNGIDLDGRTITVDRAQPQQGSSRDDGDRYRERGRDRERDRDYRGGRGSNDGGCFNCGKPGHFARECPSGEGGRGGGGRYGGRESRYGGSGGGGHYGPDRNGDRSSGGRSRDGGSRGDSGSDRYYRDRDRAGPYERRGSGGGYR