Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35214.t1 | KEH31438.1 | 96.5 | 85 | 3 | 0 | 1 | 85 | 1 | 85 | 1.30E-37 | 165.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35214.t1 | A0A072UQY6 | 96.5 | 85 | 3 | 0 | 1 | 85 | 1 | 85 | 9.3e-38 | 165.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene35214 | 0.871613 | 5.98E-67 | -1.69E-46 |
| MS.gene049340 | MS.gene35214 | 0.825718 | 4.02E-54 | -1.69E-46 |
| MS.gene049341 | MS.gene35214 | 0.85347 | 2.34E-61 | -1.69E-46 |
| MS.gene049653 | MS.gene35214 | 0.801924 | 7.16E-49 | -1.69E-46 |
| MS.gene049756 | MS.gene35214 | 0.814623 | 1.40E-51 | -1.69E-46 |
| MS.gene050277 | MS.gene35214 | 0.925853 | 1.04E-90 | -1.69E-46 |
| MS.gene050303 | MS.gene35214 | 0.829914 | 3.94E-55 | -1.69E-46 |
| MS.gene050320 | MS.gene35214 | 0.817207 | 3.72E-52 | -1.69E-46 |
| MS.gene050437 | MS.gene35214 | 0.827098 | 1.89E-54 | -1.69E-46 |
| MS.gene050689 | MS.gene35214 | 0.805254 | 1.46E-49 | -1.69E-46 |
| MS.gene050752 | MS.gene35214 | 0.812211 | 4.75E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene35214 | 0.800266 | 1.56E-48 | -1.69E-46 |
| MS.gene050815 | MS.gene35214 | 0.839912 | 1.19E-57 | -1.69E-46 |
| MS.gene050838 | MS.gene35214 | 0.813403 | 2.61E-51 | -1.69E-46 |
| MS.gene050916 | MS.gene35214 | 0.813453 | 2.54E-51 | -1.69E-46 |
| MS.gene051063 | MS.gene35214 | 0.854284 | 1.36E-61 | -1.69E-46 |
| MS.gene051241 | MS.gene35214 | 0.828483 | 8.77E-55 | -1.69E-46 |
| MS.gene051243 | MS.gene35214 | 0.846469 | 2.13E-59 | -1.69E-46 |
| MS.gene051245 | MS.gene35214 | 0.864114 | 1.53E-64 | -1.69E-46 |
| MS.gene051328 | MS.gene35214 | 0.858742 | 6.69E-63 | -1.69E-46 |
| MS.gene051330 | MS.gene35214 | 0.817451 | 3.28E-52 | -1.69E-46 |
| MS.gene051349 | MS.gene35214 | 0.81435 | 1.61E-51 | -1.69E-46 |
| MS.gene05134 | MS.gene35214 | 0.867722 | 1.11E-65 | -1.69E-46 |
| MS.gene051451 | MS.gene35214 | 0.806585 | 7.66E-50 | -1.69E-46 |
| MS.gene051633 | MS.gene35214 | 0.8755 | 2.94E-68 | -1.69E-46 |
| MS.gene051746 | MS.gene35214 | 0.876526 | 1.30E-68 | -1.69E-46 |
| MS.gene051748 | MS.gene35214 | 0.873676 | 1.22E-67 | -1.69E-46 |
| MS.gene051756 | MS.gene35214 | 0.815856 | 7.46E-52 | -1.69E-46 |
| MS.gene051757 | MS.gene35214 | 0.832409 | 9.61E-56 | -1.69E-46 |
| MS.gene051850 | MS.gene35214 | 0.817383 | 3.39E-52 | -1.69E-46 |
| MS.gene051903 | MS.gene35214 | 0.891328 | 4.39E-74 | -1.69E-46 |
| MS.gene052133 | MS.gene35214 | 0.818542 | 1.86E-52 | -1.69E-46 |
| MS.gene052286 | MS.gene35214 | 0.858086 | 1.05E-62 | -1.69E-46 |
| MS.gene052302 | MS.gene35214 | 0.847417 | 1.17E-59 | -1.69E-46 |
| MS.gene052376 | MS.gene35214 | 0.843246 | 1.58E-58 | -1.69E-46 |
| MS.gene052856 | MS.gene35214 | 0.802992 | 4.31E-49 | -1.69E-46 |
| MS.gene052879 | MS.gene35214 | 0.808806 | 2.58E-50 | -1.69E-46 |
| MS.gene052947 | MS.gene35214 | 0.885151 | 1.04E-71 | -1.69E-46 |
| MS.gene053109 | MS.gene35214 | 0.852627 | 4.08E-61 | -1.69E-46 |
| MS.gene053132 | MS.gene35214 | 0.804085 | 2.56E-49 | -1.69E-46 |
| MS.gene053437 | MS.gene35214 | 0.809499 | 1.84E-50 | -1.69E-46 |
| MS.gene053590 | MS.gene35214 | 0.804914 | 1.72E-49 | -1.69E-46 |
| MS.gene054403 | MS.gene35214 | 0.809084 | 2.25E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene35214 | 0.807331 | 5.33E-50 | -1.69E-46 |
| MS.gene055133 | MS.gene35214 | 0.805562 | 1.26E-49 | -1.69E-46 |
| MS.gene055162 | MS.gene35214 | 0.866355 | 3.03E-65 | -1.69E-46 |
| MS.gene055424 | MS.gene35214 | 0.815917 | 7.23E-52 | -1.69E-46 |
| MS.gene055441 | MS.gene35214 | 0.808508 | 2.99E-50 | -1.69E-46 |
| MS.gene055591 | MS.gene35214 | -0.812626 | 3.86E-51 | -1.69E-46 |
| MS.gene055808 | MS.gene35214 | 0.801509 | 8.71E-49 | -1.69E-46 |
| MS.gene055912 | MS.gene35214 | 0.808773 | 2.63E-50 | -1.69E-46 |
| MS.gene056069 | MS.gene35214 | -0.812039 | 5.18E-51 | -1.69E-46 |
| MS.gene056432 | MS.gene35214 | 0.832727 | 8.01E-56 | -1.69E-46 |
| MS.gene056580 | MS.gene35214 | 0.813782 | 2.15E-51 | -1.69E-46 |
| MS.gene056601 | MS.gene35214 | 0.869506 | 2.94E-66 | -1.69E-46 |
| MS.gene056631 | MS.gene35214 | 0.896464 | 3.60E-76 | -1.69E-46 |
| MS.gene056760 | MS.gene35214 | 0.847931 | 8.46E-60 | -1.69E-46 |
| MS.gene057057 | MS.gene35214 | 0.872198 | 3.82E-67 | -1.69E-46 |
| MS.gene057079 | MS.gene35214 | 0.824449 | 8.02E-54 | -1.69E-46 |
| MS.gene057094 | MS.gene35214 | 0.846572 | 2.00E-59 | -1.69E-46 |
| MS.gene057140 | MS.gene35214 | 0.8805 | 5.21E-70 | -1.69E-46 |
| MS.gene057234 | MS.gene35214 | 0.894818 | 1.72E-75 | -1.69E-46 |
| MS.gene057705 | MS.gene35214 | 0.862786 | 3.96E-64 | -1.69E-46 |
| MS.gene05795 | MS.gene35214 | 0.83339 | 5.48E-56 | -1.69E-46 |
| MS.gene058055 | MS.gene35214 | 0.867175 | 1.66E-65 | -1.69E-46 |
| MS.gene058084 | MS.gene35214 | 0.830928 | 2.23E-55 | -1.69E-46 |
| MS.gene05809 | MS.gene35214 | 0.800227 | 1.59E-48 | -1.69E-46 |
| MS.gene058182 | MS.gene35214 | 0.817041 | 4.05E-52 | -1.69E-46 |
| MS.gene058238 | MS.gene35214 | 0.880652 | 4.60E-70 | -1.69E-46 |
| MS.gene058798 | MS.gene35214 | 0.829883 | 4.01E-55 | -1.69E-46 |
| MS.gene058906 | MS.gene35214 | 0.92338 | 2.87E-89 | -1.69E-46 |
| MS.gene059151 | MS.gene35214 | 0.821864 | 3.22E-53 | -1.69E-46 |
| MS.gene059317 | MS.gene35214 | 0.821782 | 3.36E-53 | -1.69E-46 |
| MS.gene059898 | MS.gene35214 | 0.896056 | 5.32E-76 | -1.69E-46 |
| MS.gene06001 | MS.gene35214 | 0.897918 | 8.81E-77 | -1.69E-46 |
| MS.gene060165 | MS.gene35214 | 0.826958 | 2.04E-54 | -1.69E-46 |
| MS.gene060429 | MS.gene35214 | 0.828718 | 7.69E-55 | -1.69E-46 |
| MS.gene060586 | MS.gene35214 | 0.826881 | 2.13E-54 | -1.69E-46 |
| MS.gene060619 | MS.gene35214 | 0.820872 | 5.45E-53 | -1.69E-46 |
| MS.gene060917 | MS.gene35214 | 0.834497 | 2.90E-56 | -1.69E-46 |
| MS.gene061145 | MS.gene35214 | 0.841117 | 5.77E-58 | -1.69E-46 |
| MS.gene06118 | MS.gene35214 | 0.800943 | 1.14E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35214.t1 | MTR_4g094792 | 96.471 | 85 | 3 | 0 | 1 | 85 | 1 | 85 | 4.43e-57 | 170 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35214.t1 | AT4G20150 | 76.000 | 75 | 18 | 0 | 1 | 75 | 1 | 75 | 4.39e-39 | 124 |
Find 21 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCGGAGCCTTATTAGGTTT+GGG | 0.248368 | 4.4:+18609122 | MS.gene35214:CDS |
| CTGAGTCCCCAAACCTAATA+AGG | 0.285931 | 4.4:-18609129 | None:intergenic |
| CCGTCAATCAGCTTCTTAAA+TGG | 0.301085 | 4.4:+18609331 | MS.gene35214:CDS |
| AGATCCGTGGGAGCACGTTT+TGG | 0.314042 | 4.4:+18609287 | MS.gene35214:intron |
| GATCGGAGCCTTATTAGGTT+TGG | 0.322875 | 4.4:+18609121 | MS.gene35214:CDS |
| ACGGCGATCGGAGCCTTATT+AGG | 0.378847 | 4.4:+18609116 | MS.gene35214:CDS |
| CCATTTAAGAAGCTGATTGA+CGG | 0.458422 | 4.4:-18609331 | None:intergenic |
| CGTCAATCAGCTTCTTAAAT+GGG | 0.464581 | 4.4:+18609332 | MS.gene35214:CDS |
| AGATGTACTCCAACGCTCTC+AGG | 0.466035 | 4.4:+18609150 | MS.gene35214:CDS |
| GATGAATTACGACGCATGTA+AGG | 0.488766 | 4.4:-18609179 | None:intergenic |
| GTGCTAAGCGCGACGGCGAT+CGG | 0.497668 | 4.4:+18609104 | MS.gene35214:CDS |
| TGCAATTGTGTTAGATCCGT+GGG | 0.502929 | 4.4:+18609275 | MS.gene35214:intron |
| AATGTAACGTCTTTCATTAG+CGG | 0.520069 | 4.4:-18609403 | None:intergenic |
| TCGGAGCCTTATTAGGTTTG+GGG | 0.521009 | 4.4:+18609123 | MS.gene35214:CDS |
| GGGAATGGGATTGGGCGTTG+TGG | 0.541699 | 4.4:+18609308 | MS.gene35214:CDS |
| AGGAAGTTTCCTGAGAGCGT+TGG | 0.556266 | 4.4:-18609159 | None:intergenic |
| ATTCCCAAAACGTGCTCCCA+CGG | 0.568376 | 4.4:-18609291 | None:intergenic |
| TTGCAATTGTGTTAGATCCG+TGG | 0.576057 | 4.4:+18609274 | MS.gene35214:intron |
| GCTAATGAAAGACGTTACAT+TGG | 0.602826 | 4.4:+18609405 | MS.gene35214:CDS |
| TAAGATGCTTGACAAAGCTA+AGG | 0.619792 | 4.4:+18609380 | MS.gene35214:CDS |
| CAATCAGCTTCTTAAATGGG+AGG | 0.632016 | 4.4:+18609335 | MS.gene35214:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGTAACGTCTTTCATTAG+CGG | - | chr4.4:18609406-18609425 | None:intergenic | 30.0% | |
| CCATTTAAGAAGCTGATTGA+CGG | - | chr4.4:18609334-18609353 | None:intergenic | 35.0% | |
| CGTCAATCAGCTTCTTAAAT+GGG | + | chr4.4:18609332-18609351 | MS.gene35214:CDS | 35.0% | |
| GCTAATGAAAGACGTTACAT+TGG | + | chr4.4:18609405-18609424 | MS.gene35214:CDS | 35.0% | |
| TAAGATGCTTGACAAAGCTA+AGG | + | chr4.4:18609380-18609399 | MS.gene35214:CDS | 35.0% | |
| CAATCAGCTTCTTAAATGGG+AGG | + | chr4.4:18609335-18609354 | MS.gene35214:CDS | 40.0% | |
| CCGTCAATCAGCTTCTTAAA+TGG | + | chr4.4:18609331-18609350 | MS.gene35214:CDS | 40.0% | |
| GATGAATTACGACGCATGTA+AGG | - | chr4.4:18609182-18609201 | None:intergenic | 40.0% | |
| TGCAATTGTGTTAGATCCGT+GGG | + | chr4.4:18609275-18609294 | MS.gene35214:intron | 40.0% | |
| TTGCAATTGTGTTAGATCCG+TGG | + | chr4.4:18609274-18609293 | MS.gene35214:intron | 40.0% | |
| !! | ATCGGAGCCTTATTAGGTTT+GGG | + | chr4.4:18609122-18609141 | MS.gene35214:CDS | 40.0% |
| CTGAGTCCCCAAACCTAATA+AGG | - | chr4.4:18609132-18609151 | None:intergenic | 45.0% | |
| !! | GATCGGAGCCTTATTAGGTT+TGG | + | chr4.4:18609121-18609140 | MS.gene35214:CDS | 45.0% |
| !! | TCGGAGCCTTATTAGGTTTG+GGG | + | chr4.4:18609123-18609142 | MS.gene35214:CDS | 45.0% |
| !!! | CACGTTTTGGGAATGGGATT+GGG | + | chr4.4:18609300-18609319 | MS.gene35214:CDS | 45.0% |
| AGATGTACTCCAACGCTCTC+AGG | + | chr4.4:18609150-18609169 | MS.gene35214:CDS | 50.0% | |
| ATTCCCAAAACGTGCTCCCA+CGG | - | chr4.4:18609294-18609313 | None:intergenic | 50.0% | |
| ! | AGGAAGTTTCCTGAGAGCGT+TGG | - | chr4.4:18609162-18609181 | None:intergenic | 50.0% |
| !!! | GCACGTTTTGGGAATGGGAT+TGG | + | chr4.4:18609299-18609318 | MS.gene35214:CDS | 50.0% |
| !!! | TGGGAGCACGTTTTGGGAAT+GGG | + | chr4.4:18609294-18609313 | MS.gene35214:CDS | 50.0% |
| !! | ACGGCGATCGGAGCCTTATT+AGG | + | chr4.4:18609116-18609135 | MS.gene35214:CDS | 55.0% |
| !! | AGATCCGTGGGAGCACGTTT+TGG | + | chr4.4:18609287-18609306 | MS.gene35214:intron | 55.0% |
| !! | GATCCGTGGGAGCACGTTTT+GGG | + | chr4.4:18609288-18609307 | MS.gene35214:intron | 55.0% |
| !!! | GTGGGAGCACGTTTTGGGAA+TGG | + | chr4.4:18609293-18609312 | MS.gene35214:CDS | 55.0% |
| ! | GGGAATGGGATTGGGCGTTG+TGG | + | chr4.4:18609308-18609327 | MS.gene35214:CDS | 60.0% |
| GTGCTAAGCGCGACGGCGAT+CGG | + | chr4.4:18609104-18609123 | MS.gene35214:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 18609101 | 18609455 | 18609101 | ID=MS.gene35214 |
| chr4.4 | mRNA | 18609101 | 18609455 | 18609101 | ID=MS.gene35214.t1;Parent=MS.gene35214 |
| chr4.4 | exon | 18609101 | 18609191 | 18609101 | ID=MS.gene35214.t1.exon1;Parent=MS.gene35214.t1 |
| chr4.4 | CDS | 18609101 | 18609191 | 18609101 | ID=cds.MS.gene35214.t1;Parent=MS.gene35214.t1 |
| chr4.4 | exon | 18609289 | 18609455 | 18609289 | ID=MS.gene35214.t1.exon2;Parent=MS.gene35214.t1 |
| chr4.4 | CDS | 18609289 | 18609455 | 18609289 | ID=cds.MS.gene35214.t1;Parent=MS.gene35214.t1 |
| Gene Sequence |
| Protein sequence |
