AlfalfaGEDB | MS.gene067521

M. sativa cultivar XinJiangDaYe / MS.gene067521

KEGG: K02973
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015935 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00164.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067521.t1	RYR71178.1	100	136	0	0	1	136	8	143	2.00E-69	271.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067521.t1	P46297	99.3	136	1	0	1	136	7	142	1.1e-71	270.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067521.t1	A0A1S3TNG0	100.0	136	0	0	1	136	7	142	1.5e-69	271.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene067521	0.860798	1.61E-63	-1.69E-46
MS.gene049340	MS.gene067521	0.824859	6.42E-54	-1.69E-46
MS.gene049341	MS.gene067521	0.865126	7.40E-65	-1.69E-46
MS.gene049756	MS.gene067521	0.800579	1.35E-48	-1.69E-46
MS.gene049821	MS.gene067521	0.853349	2.53E-61	-1.69E-46
MS.gene049824	MS.gene067521	0.832322	1.01E-55	-1.69E-46
MS.gene049825	MS.gene067521	0.843063	1.76E-58	-1.69E-46
MS.gene049873	MS.gene067521	0.8449	5.68E-59	-1.69E-46
MS.gene050020	MS.gene067521	0.814594	1.42E-51	-1.69E-46
MS.gene050277	MS.gene067521	0.871479	6.62E-67	-1.69E-46
MS.gene050303	MS.gene067521	0.858404	8.43E-63	-1.69E-46
MS.gene050689	MS.gene067521	0.802814	4.69E-49	-1.69E-46
MS.gene050807	MS.gene067521	0.834392	3.08E-56	-1.69E-46
MS.gene050815	MS.gene067521	0.807007	6.24E-50	-1.69E-46
MS.gene050838	MS.gene067521	0.837655	4.57E-57	-1.69E-46
MS.gene050901	MS.gene067521	0.808423	3.12E-50	-1.69E-46
MS.gene050916	MS.gene067521	0.813777	2.16E-51	-1.69E-46
MS.gene051063	MS.gene067521	0.821642	3.62E-53	-1.69E-46
MS.gene051247	MS.gene067521	0.806385	8.44E-50	-1.69E-46
MS.gene051328	MS.gene067521	0.865756	4.69E-65	-1.69E-46
MS.gene051330	MS.gene067521	0.836339	9.91E-57	-1.69E-46
MS.gene05134	MS.gene067521	0.844619	6.76E-59	-1.69E-46
MS.gene051633	MS.gene067521	0.822685	2.08E-53	-1.69E-46
MS.gene051700	MS.gene067521	0.849739	2.67E-60	-1.69E-46
MS.gene051719	MS.gene067521	0.815254	1.02E-51	-1.69E-46
MS.gene051720	MS.gene067521	0.811286	7.56E-51	-1.69E-46
MS.gene051746	MS.gene067521	0.864317	1.33E-64	-1.69E-46
MS.gene051748	MS.gene067521	0.84291	1.94E-58	-1.69E-46
MS.gene051791	MS.gene067521	0.822658	2.11E-53	-1.69E-46
MS.gene051850	MS.gene067521	0.809178	2.15E-50	-1.69E-46
MS.gene05188	MS.gene067521	0.842398	2.65E-58	-1.69E-46
MS.gene051903	MS.gene067521	0.834651	2.65E-56	-1.69E-46
MS.gene051975	MS.gene067521	0.809623	1.73E-50	-1.69E-46
MS.gene052133	MS.gene067521	0.861083	1.32E-63	-1.69E-46
MS.gene052286	MS.gene067521	0.826972	2.02E-54	-1.69E-46
MS.gene052302	MS.gene067521	0.823455	1.37E-53	-1.69E-46
MS.gene052376	MS.gene067521	0.829863	4.06E-55	-1.69E-46
MS.gene052879	MS.gene067521	0.823812	1.13E-53	-1.69E-46
MS.gene052947	MS.gene067521	0.907507	4.70E-81	-1.69E-46
MS.gene053122	MS.gene067521	0.806941	6.44E-50	-1.69E-46
MS.gene053226	MS.gene067521	0.824401	8.23E-54	-1.69E-46
MS.gene053230	MS.gene067521	0.802064	6.70E-49	-1.69E-46
MS.gene053294	MS.gene067521	0.806219	9.15E-50	-1.69E-46
MS.gene053563	MS.gene067521	0.80472	1.89E-49	-1.69E-46
MS.gene053581	MS.gene067521	0.812822	3.49E-51	-1.69E-46
MS.gene053599	MS.gene067521	0.816126	6.49E-52	-1.69E-46
MS.gene05364	MS.gene067521	0.819767	9.77E-53	-1.69E-46
MS.gene055162	MS.gene067521	0.813483	2.50E-51	-1.69E-46
MS.gene055465	MS.gene067521	0.818582	1.82E-52	-1.69E-46
MS.gene055466	MS.gene067521	0.832178	1.10E-55	-1.69E-46
MS.gene055468	MS.gene067521	0.838261	3.19E-57	-1.69E-46
MS.gene055512	MS.gene067521	0.809461	1.87E-50	-1.69E-46
MS.gene055555	MS.gene067521	0.812223	4.72E-51	-1.69E-46
MS.gene055580	MS.gene067521	-0.838722	2.43E-57	-1.69E-46
MS.gene055608	MS.gene067521	0.804673	1.93E-49	-1.69E-46
MS.gene055778	MS.gene067521	0.823154	1.61E-53	-1.69E-46
MS.gene055808	MS.gene067521	0.890753	7.41E-74	-1.69E-46
MS.gene055912	MS.gene067521	0.811053	8.48E-51	-1.69E-46
MS.gene056043	MS.gene067521	-0.835765	1.39E-56	-1.69E-46
MS.gene056075	MS.gene067521	0.840466	8.55E-58	-1.69E-46
MS.gene056091	MS.gene067521	0.859566	3.79E-63	-1.69E-46
MS.gene056215	MS.gene067521	0.871411	6.98E-67	-1.69E-46
MS.gene056601	MS.gene067521	0.815737	7.93E-52	-1.69E-46
MS.gene056631	MS.gene067521	0.899963	1.18E-77	-1.69E-46
MS.gene056760	MS.gene067521	0.807132	5.87E-50	-1.69E-46
MS.gene056986	MS.gene067521	0.808345	3.24E-50	-1.69E-46
MS.gene057057	MS.gene067521	0.82582	3.80E-54	-1.69E-46
MS.gene057060	MS.gene067521	0.808585	2.88E-50	-1.69E-46
MS.gene057094	MS.gene067521	0.833261	5.90E-56	-1.69E-46
MS.gene057140	MS.gene067521	0.813356	2.67E-51	-1.69E-46
MS.gene057234	MS.gene067521	0.813761	2.17E-51	-1.69E-46
MS.gene057693	MS.gene067521	0.811614	6.41E-51	-1.69E-46
MS.gene057694	MS.gene067521	0.80208	6.65E-49	-1.69E-46
MS.gene057705	MS.gene067521	0.879546	1.14E-69	-1.69E-46
MS.gene058055	MS.gene067521	0.803605	3.22E-49	-1.69E-46
MS.gene058084	MS.gene067521	0.83408	3.69E-56	-1.69E-46
MS.gene058216	MS.gene067521	0.812203	4.77E-51	-1.69E-46
MS.gene058238	MS.gene067521	0.813085	3.06E-51	-1.69E-46
MS.gene058852	MS.gene067521	0.81261	3.89E-51	-1.69E-46
MS.gene058906	MS.gene067521	0.858791	6.46E-63	-1.69E-46
MS.gene059317	MS.gene067521	0.812131	4.95E-51	-1.69E-46
MS.gene05953	MS.gene067521	0.83222	1.07E-55	-1.69E-46
MS.gene05954	MS.gene067521	0.847608	1.04E-59	-1.69E-46
MS.gene05978	MS.gene067521	0.819117	1.37E-52	-1.69E-46
MS.gene06001	MS.gene067521	0.92072	8.95E-88	-1.69E-46
MS.gene060194	MS.gene067521	0.810163	1.32E-50	-1.69E-46
MS.gene060429	MS.gene067521	0.91151	5.61E-83	-1.69E-46
MS.gene060586	MS.gene067521	0.811163	8.03E-51	-1.69E-46
MS.gene060587	MS.gene067521	0.833346	5.62E-56	-1.69E-46
MS.gene060917	MS.gene067521	0.849859	2.47E-60	-1.69E-46
MS.gene061176	MS.gene067521	0.806772	6.99E-50	-1.69E-46
MS.gene061177	MS.gene067521	0.80629	8.84E-50	-1.69E-46
MS.gene061178	MS.gene067521	0.806218	9.15E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067521.t1	MTR_1g066860	100.000	136	0	0	1	136	1	136	1.11e-96	274
MS.gene067521.t1	MTR_1g066860	100.000	136	0	0	1	136	7	142	1.16e-96	274
MS.gene067521.t1	MTR_7g100720	94.118	136	8	0	1	136	7	142	1.59e-90	259
MS.gene067521.t1	MTR_7g061155	96.610	118	4	0	19	136	138	255	3.89e-80	236
MS.gene067521.t1	MTR_7g063460	60.000	55	20	1	48	102	28	80	1.78e-15	67.0
MS.gene067521.t1	MTR_8g447270	64.151	53	17	1	48	100	17	67	1.68e-14	65.5
MS.gene067521.t1	MTR_4g038400	67.347	49	13	1	50	98	103	148	1.81e-12	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067521.t1	AT5G02960	95.588	136	6	0	1	136	7	142	6.49e-91	259
MS.gene067521.t1	AT3G09680	93.382	136	9	0	1	136	7	142	1.07e-87	251

Find 0 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCAAATAAAATTCTAGTAAA+AGG	-	10809:16010-16029	MS.gene067521:intergenic	15.0%
!!!	GATTGTTGTTTTATTTACAG+TGG	+	10809:16054-16073	MS.gene067521:intron	25.0%
!!!	TTTTATTTACAGTGGTATTG+AGG	+	10809:16062-16081	MS.gene067521:intron	25.0%
	ACCAATAGAAAATATGAGCA+AGG	-	10809:16210-16229	MS.gene067520:intergenic	30.0%
	AGTTAGATTCAAAGTTGTGA+AGG	+	10809:16408-16427	MS.gene067521:CDS	30.0%
	TAGTTTAGACAACCATCATT+GGG	-	10809:16168-16187	MS.gene067521:intergenic	30.0%
	TGACTTTAAACTGATATGTC+TGG	+	10809:16238-16257	MS.gene067521:intron	30.0%
!	TCCTTGCTCATATTTTCTAT+TGG	+	10809:16206-16225	MS.gene067521:intron	30.0%
	ACAACTTTGAATCTAACTCC+AGG	-	10809:16406-16425	MS.gene067520:intergenic	35.0%
	ACACTTACGAATAGCTGAAT+TGG	-	10809:16097-16116	MS.gene067520:intergenic	35.0%
	AGGGTACAACTAATCAAGAA+TGG	+	10809:16120-16139	MS.gene067521:CDS	35.0%
	ATTGCTGGATTTGGAAGAAA+GGG	+	10809:16361-16380	MS.gene067521:CDS	35.0%
	CACTTACGAATAGCTGAATT+GGG	-	10809:16096-16115	MS.gene067521:intergenic	35.0%
	CATACAAGAAGTCTCATCTT+GGG	+	10809:15897-15916	MS.gene067521:CDS	35.0%
	GTAGTTTAGACAACCATCAT+TGG	-	10809:16169-16188	MS.gene067521:intergenic	35.0%
	TCATACAAGAAGTCTCATCT+TGG	+	10809:15896-15915	MS.gene067521:CDS	35.0%
!	ATTCTTGATTAGTTGTACCC+TGG	-	10809:16121-16140	MS.gene067521:intergenic	35.0%
!!!	GAAGTTTTGATTGCTGGATT+TGG	+	10809:16352-16371	MS.gene067521:CDS	35.0%
	ACAACCATCATTGGGAACAA+AGG	-	10809:16160-16179	MS.gene067520:intergenic	40.0%
	ACACACAATGTCACACAAGA+TGG	-	10809:16314-16333	MS.gene067520:intergenic	40.0%
	CTCCTTCTTCTCCTTGAAAA+GGG	-	10809:16455-16474	MS.gene067521:intergenic	40.0%
	GATGTAAGAGTGGTTAAGCT+TGG	+	10809:15979-15998	MS.gene067521:intron	40.0%
	GATTGCTGGATTTGGAAGAA+AGG	+	10809:16360-16379	MS.gene067521:CDS	40.0%
	TCAAAGTTGTGAAGGTCTCT+GGG	+	10809:16416-16435	MS.gene067521:CDS	40.0%
	TCCTCGAAAAGATGTAAGAG+TGG	+	10809:15969-15988	MS.gene067521:intron	40.0%
	TCTCCTTCTTCTCCTTGAAA+AGG	-	10809:16456-16475	MS.gene067521:intergenic	40.0%
	TTCAAAGTTGTGAAGGTCTC+TGG	+	10809:16415-16434	MS.gene067521:CDS	40.0%
!	ACCACTCTTACATCTTTTCG+AGG	-	10809:15973-15992	MS.gene067521:intergenic	40.0%
!!!	CAGGATGAAGTTTTGATTGC+TGG	+	10809:16346-16365	MS.gene067521:intron	40.0%
	AGCTATTCGTAAGTGTGCCA+GGG	+	10809:16101-16120	MS.gene067521:CDS	45.0%
	AGTCTCATCTTGGGAATGAG+TGG	+	10809:15906-15925	MS.gene067521:CDS	45.0%
	CAAAGTTGTGAAGGTCTCTG+GGG	+	10809:16417-16436	MS.gene067521:CDS	45.0%
	GTGACATTGTGTGTGACTGA+TGG	+	10809:16320-16339	MS.gene067521:intron	45.0%
!	GAGTGGAAGAAACCTTTTGC+TGG	+	10809:15923-15942	MS.gene067521:CDS	45.0%
!	GTCTCTTCTTGCCCTTTTCA+AGG	+	10809:16441-16460	MS.gene067521:CDS	45.0%
!!	TGCCCTTTTCAAGGAGAAGA+AGG	+	10809:16450-16469	MS.gene067521:CDS	45.0%
	CAGCTATTCGTAAGTGTGCC+AGG	+	10809:16100-16119	MS.gene067521:CDS	50.0%
	CATGCTGTGGGAGATATTCC+TGG	+	10809:16385-16404	MS.gene067521:CDS	50.0%
	GAGGACAATTCCCTTTGCGT+GGG	-	10809:15954-15973	MS.gene067521:intergenic	50.0%
	GCTGCCTTTGTTCCCAATGA+TGG	+	10809:16153-16172	MS.gene067521:CDS	50.0%
	GGAAGAAAGGGTCATGCTGT+GGG	+	10809:16373-16392	MS.gene067521:CDS	50.0%
	TGGAAGAAAGGGTCATGCTG+TGG	+	10809:16372-16391	MS.gene067521:CDS	50.0%
	TGTGTGTGACTGATGGTTGC+AGG	+	10809:16327-16346	MS.gene067521:intron	50.0%
	CGAGGACAATTCCCTTTGCG+TGG	-	10809:15955-15974	MS.gene067521:intergenic	55.0%
	GCGTGGGAAGAACCAGCAAA+AGG	-	10809:15938-15957	MS.gene067521:intergenic	55.0%
!	GCTGGTTCTTCCCACGCAAA+GGG	+	10809:15941-15960	MS.gene067521:CDS	55.0%
!!	TGCTGGTTCTTCCCACGCAA+AGG	+	10809:15940-15959	MS.gene067521:CDS	55.0%
	AGCTGAAGTCTCACCGCAGG+AGG	+	10809:15855-15874	MS.gene067521:CDS	60.0%
	AGTCTCACCGCAGGAGGCAA+AGG	+	10809:15861-15880	MS.gene067521:CDS	60.0%
	GCAAGCTGAAGTCTCACCGC+AGG	+	10809:15852-15871	MS.gene067521:CDS	60.0%
	TCACCGCAGGAGGCAAAGGT+GGG	+	10809:15865-15884	MS.gene067521:CDS	60.0%
	CAGCCCACCTTTGCCTCCTG+CGG	-	10809:15871-15890	MS.gene067521:intergenic	65.0%
	CTCACCGCAGGAGGCAAAGG+TGG	+	10809:15864-15883	MS.gene067521:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
10809	gene	15839	16489	15839	ID=MS.gene067521
10809	mRNA	15839	16489	15839	ID=MS.gene067521.t1;Parent=MS.gene067521
10809	exon	15839	15981	15839	ID=MS.gene067521.t1.exon1;Parent=MS.gene067521.t1
10809	CDS	15839	15981	15839	ID=cds.MS.gene067521.t1;Parent=MS.gene067521.t1
10809	exon	16074	16200	16074	ID=MS.gene067521.t1.exon2;Parent=MS.gene067521.t1
10809	CDS	16074	16200	16074	ID=cds.MS.gene067521.t1;Parent=MS.gene067521.t1
10809	exon	16349	16489	16349	ID=MS.gene067521.t1.exon3;Parent=MS.gene067521.t1
10809	CDS	16349	16489	16349	ID=cds.MS.gene067521.t1;Parent=MS.gene067521.t1

Gene Sequence

>MS.gene067521

ATGGGAGCTGCTCGCAAGCTGAAGTCTCACCGCAGGAGGCAAAGGTGGGCTGACAAGTCATACAAGAAGTCTCATCTTGGGAATGAGTGGAAGAAACCTTTTGCTGGTTCTTCCCACGCAAAGGGAATTGTCCTCGAAAAGATGTAAGAGTGGTTAAGCTTGGTATTTCCTTTTACTAGAATTTTATTTGATATGAACATGTGATGCTAAGAAATGATTGTTGTTTTATTTACAGTGGTATTGAGGCTAAACAGCCCAATTCAGCTATTCGTAAGTGTGCCAGGGTACAACTAATCAAGAATGGAAAGAAAATTGCTGCCTTTGTTCCCAATGATGGTTGTCTAAACTACATCGAAGAAAATGTAAGTCCTTGCTCATATTTTCTATTGGTTTGATCAGTGACTTTAAACTGATATGTCTGGAACTGTCTTAAATTGTTTTTCATATCTTATTAGTAATTCGTTGTTTGTATCCATCTTGTGTGACATTGTGTGTGACTGATGGTTGCAGGATGAAGTTTTGATTGCTGGATTTGGAAGAAAGGGTCATGCTGTGGGAGATATTCCTGGAGTTAGATTCAAAGTTGTGAAGGTCTCTGGGGTGTCTCTTCTTGCCCTTTTCAAGGAGAAGAAGGAGAAGCCAAGATCTTAG

Protein sequence

>MS.gene067521.t1

MGAARKLKSHRRRQRWADKSYKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQPNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFKVVKVSGVSLLALFKEKKEKPRS