AlfalfaGEDB | MS.gene71215

M. sativa cultivar XinJiangDaYe / MS.gene71215

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71215.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71215.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71215.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene71215	0.873048	1.99E-67	-1.69E-46
MS.gene049340	MS.gene71215	0.810674	1.03E-50	-1.69E-46
MS.gene049341	MS.gene71215	0.845577	3.72E-59	-1.69E-46
MS.gene049821	MS.gene71215	0.87241	3.25E-67	-1.69E-46
MS.gene050020	MS.gene71215	0.831401	1.70E-55	-1.69E-46
MS.gene050277	MS.gene71215	0.845867	3.11E-59	-1.69E-46
MS.gene050437	MS.gene71215	0.830828	2.36E-55	-1.69E-46
MS.gene050689	MS.gene71215	0.849571	2.97E-60	-1.69E-46
MS.gene050752	MS.gene71215	0.818418	1.98E-52	-1.69E-46
MS.gene050807	MS.gene71215	0.818377	2.02E-52	-1.69E-46
MS.gene050808	MS.gene71215	0.804906	1.73E-49	-1.69E-46
MS.gene050815	MS.gene71215	0.802134	6.48E-49	-1.69E-46
MS.gene050838	MS.gene71215	0.854746	1.00E-61	-1.69E-46
MS.gene050901	MS.gene71215	0.832149	1.11E-55	-1.69E-46
MS.gene050955	MS.gene71215	0.81098	8.80E-51	-1.69E-46
MS.gene051003	MS.gene71215	0.819859	9.31E-53	-1.69E-46
MS.gene051233	MS.gene71215	0.80409	2.55E-49	-1.69E-46
MS.gene051234	MS.gene71215	0.812442	4.23E-51	-1.69E-46
MS.gene051241	MS.gene71215	0.834353	3.15E-56	-1.69E-46
MS.gene051243	MS.gene71215	0.83184	1.33E-55	-1.69E-46
MS.gene051328	MS.gene71215	0.875796	2.32E-68	-1.69E-46
MS.gene051343	MS.gene71215	0.821978	3.03E-53	-1.69E-46
MS.gene05134	MS.gene71215	0.843884	1.06E-58	-1.69E-46
MS.gene05157	MS.gene71215	0.816956	4.23E-52	-1.69E-46
MS.gene051700	MS.gene71215	0.800624	1.32E-48	-1.69E-46
MS.gene051789	MS.gene71215	0.801579	8.42E-49	-1.69E-46
MS.gene05188	MS.gene71215	0.827169	1.81E-54	-1.69E-46
MS.gene051903	MS.gene71215	0.813745	2.19E-51	-1.69E-46
MS.gene051975	MS.gene71215	0.825593	4.31E-54	-1.69E-46
MS.gene052133	MS.gene71215	0.874168	8.34E-68	-1.69E-46
MS.gene052516	MS.gene71215	0.808087	3.68E-50	-1.69E-46
MS.gene052879	MS.gene71215	0.827058	1.93E-54	-1.69E-46
MS.gene052880	MS.gene71215	0.813501	2.48E-51	-1.69E-46
MS.gene052947	MS.gene71215	0.873779	1.13E-67	-1.69E-46
MS.gene053058	MS.gene71215	0.820138	8.03E-53	-1.69E-46
MS.gene053122	MS.gene71215	0.80634	8.63E-50	-1.69E-46
MS.gene053563	MS.gene71215	0.814569	1.44E-51	-1.69E-46
MS.gene053581	MS.gene71215	0.847398	1.19E-59	-1.69E-46
MS.gene053590	MS.gene71215	0.8074	5.15E-50	-1.69E-46
MS.gene05364	MS.gene71215	0.81701	4.12E-52	-1.69E-46
MS.gene054403	MS.gene71215	0.81691	4.33E-52	-1.69E-46
MS.gene055162	MS.gene71215	0.822479	2.32E-53	-1.69E-46
MS.gene055466	MS.gene71215	0.805848	1.10E-49	-1.69E-46
MS.gene055468	MS.gene71215	0.818157	2.27E-52	-1.69E-46
MS.gene055512	MS.gene71215	0.804683	1.92E-49	-1.69E-46
MS.gene055808	MS.gene71215	0.804185	2.44E-49	-1.69E-46
MS.gene055912	MS.gene71215	0.837996	3.74E-57	-1.69E-46
MS.gene05608	MS.gene71215	0.804707	1.90E-49	-1.69E-46
MS.gene056215	MS.gene71215	0.858033	1.09E-62	-1.69E-46
MS.gene056580	MS.gene71215	0.835604	1.52E-56	-1.69E-46
MS.gene056631	MS.gene71215	0.848427	6.17E-60	-1.69E-46
MS.gene056760	MS.gene71215	0.807293	5.42E-50	-1.69E-46
MS.gene05712	MS.gene71215	0.802364	5.81E-49	-1.69E-46
MS.gene057234	MS.gene71215	0.822045	2.92E-53	-1.69E-46
MS.gene057693	MS.gene71215	0.813648	2.30E-51	-1.69E-46
MS.gene057694	MS.gene71215	0.852469	4.52E-61	-1.69E-46
MS.gene057705	MS.gene71215	0.883308	5.01E-71	-1.69E-46
MS.gene058054	MS.gene71215	0.809129	2.20E-50	-1.69E-46
MS.gene058055	MS.gene71215	0.829339	5.44E-55	-1.69E-46
MS.gene058238	MS.gene71215	0.811821	5.78E-51	-1.69E-46
MS.gene058852	MS.gene71215	0.805194	1.50E-49	-1.69E-46
MS.gene058906	MS.gene71215	0.825973	3.50E-54	-1.69E-46
MS.gene058976	MS.gene71215	0.83306	6.62E-56	-1.69E-46
MS.gene059006	MS.gene71215	0.802318	5.94E-49	-1.69E-46
MS.gene059146	MS.gene71215	0.800228	1.59E-48	-1.69E-46
MS.gene05978	MS.gene71215	0.839391	1.63E-57	-1.69E-46
MS.gene06001	MS.gene71215	0.844085	9.41E-59	-1.69E-46
MS.gene060429	MS.gene71215	0.874899	4.71E-68	-1.69E-46
MS.gene060587	MS.gene71215	0.824022	1.01E-53	-1.69E-46
MS.gene060917	MS.gene71215	0.842861	2.00E-58	-1.69E-46
MS.gene061176	MS.gene71215	0.829009	6.54E-55	-1.69E-46
MS.gene061177	MS.gene71215	0.829539	4.87E-55	-1.69E-46
MS.gene061178	MS.gene71215	0.829024	6.49E-55	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene71215	MS.gene048746	PPI
MS.gene71215	MS.gene028629	PPI
MS.gene71215	MS.gene58854	PPI
MS.gene71215	MS.gene043967	PPI
MS.gene71215	MS.gene31196	PPI
MS.gene071136	MS.gene71215	PPI
MS.gene71215	MS.gene060465	PPI
MS.gene78578	MS.gene71215	PPI
MS.gene71215	MS.gene035897	PPI
MS.gene71215	MS.gene049705	PPI
MS.gene71215	MS.gene061870	PPI
MS.gene71215	MS.gene05608	PPI
MS.gene71215	MS.gene070241	PPI
MS.gene71215	MS.gene035896	PPI
MS.gene71215	MS.gene88681	PPI
MS.gene71215	MS.gene82546	PPI
MS.gene71215	MS.gene60093	PPI
MS.gene035787	MS.gene71215	PPI
MS.gene04067	MS.gene71215	PPI
MS.gene71215	MS.gene08122	PPI
MS.gene71215	MS.gene09873	PPI
MS.gene71215	MS.gene035718	PPI
MS.gene71215	MS.gene022932	PPI
MS.gene71215	MS.gene010220	PPI
MS.gene71215	MS.gene04972	PPI
MS.gene71215	MS.gene004121	PPI
MS.gene71215	MS.gene62520	PPI
MS.gene71215	MS.gene08053	PPI
MS.gene71215	MS.gene018578	PPI
MS.gene71215	MS.gene035721	PPI
MS.gene71215	MS.gene009027	PPI
MS.gene71215	MS.gene04064	PPI
MS.gene71215	MS.gene018580	PPI
MS.gene71215	MS.gene004124	PPI
MS.gene71215	MS.gene071136	PPI
MS.gene71215	MS.gene88680	PPI
MS.gene070241	MS.gene71215	PPI
MS.gene056215	MS.gene71215	PPI
MS.gene71215	MS.gene072493	PPI
MS.gene71215	MS.gene04052	PPI
MS.gene71215	MS.gene84536	PPI
MS.gene71215	MS.gene061866	PPI
MS.gene061876	MS.gene71215	PPI
MS.gene71215	MS.gene64650	PPI
MS.gene71215	MS.gene79195	PPI
MS.gene71215	MS.gene048705	PPI
MS.gene71215	MS.gene061876	PPI
MS.gene71215	MS.gene061874	PPI
MS.gene71215	MS.gene04576	PPI
MS.gene71215	MS.gene056215	PPI
MS.gene71215	MS.gene061869	PPI
MS.gene060463	MS.gene71215	PPI
MS.gene71215	MS.gene04452	PPI
MS.gene71215	MS.gene070244	PPI
MS.gene71215	MS.gene038469	PPI
MS.gene71215	MS.gene061867	PPI
MS.gene71215	MS.gene008276	PPI
MS.gene71215	MS.gene30920	PPI
MS.gene71215	MS.gene012391	PPI
MS.gene71215	MS.gene05619	PPI
MS.gene71215	MS.gene73136	PPI
MS.gene71215	MS.gene049821	PPI
MS.gene71215	MS.gene78578	PPI
MS.gene71215	MS.gene04068	PPI
MS.gene71215	MS.gene022946	PPI
MS.gene71215	MS.gene035719	PPI
MS.gene71215	MS.gene05623	PPI
MS.gene71215	MS.gene061872	PPI
MS.gene71215	MS.gene89488	PPI
MS.gene012391	MS.gene71215	PPI
MS.gene71215	MS.gene66225	PPI
MS.gene71215	MS.gene035720	PPI
MS.gene71215	MS.gene70955	PPI
MS.gene71215	MS.gene61017	PPI
MS.gene71215	MS.gene09871	PPI
MS.gene71215	MS.gene012390	PPI
MS.gene71215	MS.gene08121	PPI
MS.gene71215	MS.gene64920	PPI
MS.gene71215	MS.gene04067	PPI
MS.gene71215	MS.gene064557	PPI
MS.gene71215	MS.gene035787	PPI
MS.gene71215	MS.gene04434	PPI
MS.gene71215	MS.gene070907	PPI
MS.gene71215	MS.gene04392	PPI
MS.gene71215	MS.gene060463	PPI
MS.gene71215	MS.gene89487	PPI
MS.gene71215	MS.gene010219	PPI
MS.gene71215	MS.gene04433	PPI
MS.gene71215	MS.gene040383	PPI
MS.gene71215	MS.gene68545	PPI
MS.gene71215	MS.gene070242	PPI
MS.gene71215	MS.gene016624	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71215.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene71215.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene71215.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene71215.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene71215.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene71215.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene71215.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene71215.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene71215.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene71215.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene71215.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene71215.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene71215.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene71215.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene71215.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene71215.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71215.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene71215.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene71215.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene71215.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene71215.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene71215.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene71215.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene71215.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene71215.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene71215.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene71215.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 30 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCCTGCGATTCGTCGTT+TGG	0.336158	8.3:-36532729	MS.gene71215:CDS
GGGAGAACTCTTTACGGTTT+CGG	0.376970	8.3:-36532539	MS.gene71215:CDS
TCGCAGGCTTTGTTATTCCT+TGG	0.407291	8.3:+36532741	None:intergenic
AAAATGTCAGGTCGCGGAAA+AGG	0.432832	8.3:-36532824	MS.gene71215:CDS
GGAGGAAAGGGATTGGGAAA+AGG	0.436575	8.3:-36532803	MS.gene71215:CDS
AGACAGGGGAGAACTCTTTA+CGG	0.442868	8.3:-36532545	MS.gene71215:CDS
TGTTTATGCTTTGAAGAGAC+AGG	0.461410	8.3:-36532561	MS.gene71215:CDS
GAGGAAAACCGTTACGGCTA+TGG	0.462806	8.3:-36532588	MS.gene71215:CDS
GGAAAAGGAGGAAAGGGATT+GGG	0.479340	8.3:-36532809	MS.gene71215:CDS
CGGAAAAGGAGGAAAGGGAT+TGG	0.487643	8.3:-36532810	MS.gene71215:CDS
CGTCGTTTGGCTCGAAGAGG+AGG	0.516708	8.3:-36532716	MS.gene71215:CDS
TTACTTACACTGAGCATGCT+AGG	0.518049	8.3:-36532610	MS.gene71215:CDS
GGAAAGGGATTGGGAAAAGG+AGG	0.526255	8.3:-36532800	MS.gene71215:CDS
TGCTAGGAGGAAAACCGTTA+CGG	0.546899	8.3:-36532594	MS.gene71215:CDS
CGAGCCAAACGACGAATCGC+AGG	0.562444	8.3:+36532725	None:intergenic
GGTCGCGGAAAAGGAGGAAA+GGG	0.574670	8.3:-36532815	MS.gene71215:CDS
AGGAGCAAAGAGGCACAGAA+AGG	0.576895	8.3:-36532780	MS.gene71215:CDS
ATGTCAGGTCGCGGAAAAGG+AGG	0.578066	8.3:-36532821	MS.gene71215:CDS
GTTTATGCTTTGAAGAGACA+GGG	0.590468	8.3:-36532560	MS.gene71215:CDS
GGAGGTGTGAAGAGAATCAG+TGG	0.594114	8.3:-36532698	MS.gene71215:CDS
AGGTCGCGGAAAAGGAGGAA+AGG	0.594714	8.3:-36532816	MS.gene71215:CDS
AACAACATCCATAGCCGTAA+CGG	0.596499	8.3:+36532580	None:intergenic
AGAACTCTTTACGGTTTCGG+TGG	0.599890	8.3:-36532536	MS.gene71215:CDS
ATTCGTCGTTTGGCTCGAAG+AGG	0.614299	8.3:-36532719	MS.gene71215:CDS
GTTCTTCGTGACAACATCCA+AGG	0.625870	8.3:-36532758	MS.gene71215:CDS
TTTATGCTTTGAAGAGACAG+GGG	0.664122	8.3:-36532559	MS.gene71215:CDS
CTTATCTATGAAGAAACTCG+CGG	0.683519	8.3:-36532674	MS.gene71215:CDS
CATCACGAATAACGTTCTCG+AGG	0.697048	8.3:+36532636	None:intergenic
CTTACACTGAGCATGCTAGG+AGG	0.700541	8.3:-36532607	MS.gene71215:CDS
TGGGAAAAGGAGGAGCAAAG+AGG	0.707785	8.3:-36532790	MS.gene71215:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTTATCTATGAAGAAACTCG+CGG	-	chr8.3:36532679-36532698	MS.gene71215:CDS	35.0%
!!	GTTTATGCTTTGAAGAGACA+GGG	-	chr8.3:36532793-36532812	MS.gene71215:CDS	35.0%
!!	TGTTTATGCTTTGAAGAGAC+AGG	-	chr8.3:36532792-36532811	MS.gene71215:CDS	35.0%
!!	TTTATGCTTTGAAGAGACAG+GGG	-	chr8.3:36532794-36532813	MS.gene71215:CDS	35.0%
	AACAACATCCATAGCCGTAA+CGG	+	chr8.3:36532776-36532795	None:intergenic	40.0%
	TTACTTACACTGAGCATGCT+AGG	-	chr8.3:36532743-36532762	MS.gene71215:CDS	40.0%
	AGAACTCTTTACGGTTTCGG+TGG	-	chr8.3:36532817-36532836	MS.gene71215:CDS	45.0%
	AGACAGGGGAGAACTCTTTA+CGG	-	chr8.3:36532808-36532827	MS.gene71215:CDS	45.0%
	CATCACGAATAACGTTCTCG+AGG	+	chr8.3:36532720-36532739	None:intergenic	45.0%
	GGAAAAGGAGGAAAGGGATT+GGG	-	chr8.3:36532544-36532563	MS.gene71215:CDS	45.0%
	GGGAGAACTCTTTACGGTTT+CGG	-	chr8.3:36532814-36532833	MS.gene71215:CDS	45.0%
	GTTCTTCGTGACAACATCCA+AGG	-	chr8.3:36532595-36532614	MS.gene71215:CDS	45.0%
	TGCTAGGAGGAAAACCGTTA+CGG	-	chr8.3:36532759-36532778	MS.gene71215:CDS	45.0%
!!	TCGCAGGCTTTGTTATTCCT+TGG	+	chr8.3:36532615-36532634	None:intergenic	45.0%
	AAAGCCTGCGATTCGTCGTT+TGG	-	chr8.3:36532624-36532643	MS.gene71215:CDS	50.0%
	CGGAAAAGGAGGAAAGGGAT+TGG	-	chr8.3:36532543-36532562	MS.gene71215:CDS	50.0%
	CTTACACTGAGCATGCTAGG+AGG	-	chr8.3:36532746-36532765	MS.gene71215:CDS	50.0%
	GAGGAAAACCGTTACGGCTA+TGG	-	chr8.3:36532765-36532784	MS.gene71215:CDS	50.0%
	GGAAAGGGATTGGGAAAAGG+AGG	-	chr8.3:36532553-36532572	MS.gene71215:CDS	50.0%
	GGAGGAAAGGGATTGGGAAA+AGG	-	chr8.3:36532550-36532569	MS.gene71215:CDS	50.0%
	TGGGAAAAGGAGGAGCAAAG+AGG	-	chr8.3:36532563-36532582	MS.gene71215:CDS	50.0%
!	AGGAGCAAAGAGGCACAGAA+AGG	-	chr8.3:36532573-36532592	MS.gene71215:CDS	50.0%
!	ATTCGTCGTTTGGCTCGAAG+AGG	-	chr8.3:36532634-36532653	MS.gene71215:CDS	50.0%
!	GGAGGTGTGAAGAGAATCAG+TGG	-	chr8.3:36532655-36532674	MS.gene71215:CDS	50.0%
	AGGTCGCGGAAAAGGAGGAA+AGG	-	chr8.3:36532537-36532556	MS.gene71215:CDS	55.0%
	ATGTCAGGTCGCGGAAAAGG+AGG	-	chr8.3:36532532-36532551	MS.gene71215:CDS	55.0%
	GGTCGCGGAAAAGGAGGAAA+GGG	-	chr8.3:36532538-36532557	MS.gene71215:CDS	55.0%
	CGAGCCAAACGACGAATCGC+AGG	+	chr8.3:36532631-36532650	None:intergenic	60.0%
!	CGTCGTTTGGCTCGAAGAGG+AGG	-	chr8.3:36532637-36532656	MS.gene71215:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	36532532	36532843	36532532	ID=MS.gene71215
chr8.3	mRNA	36532532	36532843	36532532	ID=MS.gene71215.t1;Parent=MS.gene71215
chr8.3	exon	36532532	36532843	36532532	ID=MS.gene71215.t1.exon1;Parent=MS.gene71215.t1
chr8.3	CDS	36532532	36532843	36532532	ID=cds.MS.gene71215.t1;Parent=MS.gene71215.t1

Gene Sequence	>MS.gene71215 ATGTCAGGTCGCGGAAAAGGAGGAAAGGGATTGGGAAAAGGAGGAGCAAAGAGGCACAGAAAGGTTCTTCGTGACAACATCCAAGGAATAACAAAGCCTGCGATTCGTCGTTTGGCTCGAAGAGGAGGTGTGAAGAGAATCAGTGGTCTTATCTATGAAGAAACTCGCGGTGTTCTCAAGATCTTCCTCGAGAACGTTATTCGTGATGCTGTTACTTACACTGAGCATGCTAGGAGGAAAACCGTTACGGCTATGGATGTTGTTTATGCTTTGAAGAGACAGGGGAGAACTCTTTACGGTTTCGGTGGTTGA
Protein sequence	>MS.gene71215.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG