Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08428.t1 | XP_013446572.1 | 100 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 3.30E-47 | 197.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08428.t1 | I3SCJ8 | 100.0 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 2.4e-47 | 197.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene08428 | 0.822533 | 2.25E-53 | -1.69E-46 |
| MS.gene049255 | MS.gene08428 | 0.881439 | 2.40E-70 | -1.69E-46 |
| MS.gene049340 | MS.gene08428 | 0.830099 | 3.55E-55 | -1.69E-46 |
| MS.gene049341 | MS.gene08428 | 0.89081 | 7.03E-74 | -1.69E-46 |
| MS.gene049382 | MS.gene08428 | 0.801296 | 9.63E-49 | -1.69E-46 |
| MS.gene049756 | MS.gene08428 | 0.808503 | 3.00E-50 | -1.69E-46 |
| MS.gene049821 | MS.gene08428 | 0.802954 | 4.39E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene08428 | 0.816311 | 5.90E-52 | -1.69E-46 |
| MS.gene049979 | MS.gene08428 | 0.800875 | 1.17E-48 | -1.69E-46 |
| MS.gene050020 | MS.gene08428 | 0.813212 | 2.87E-51 | -1.69E-46 |
| MS.gene050277 | MS.gene08428 | 0.885717 | 6.39E-72 | -1.69E-46 |
| MS.gene050303 | MS.gene08428 | 0.827178 | 1.81E-54 | -1.69E-46 |
| MS.gene050437 | MS.gene08428 | 0.818364 | 2.04E-52 | -1.69E-46 |
| MS.gene050689 | MS.gene08428 | 0.837602 | 4.72E-57 | -1.69E-46 |
| MS.gene050719 | MS.gene08428 | 0.808511 | 2.99E-50 | -1.69E-46 |
| MS.gene050721 | MS.gene08428 | 0.811432 | 7.02E-51 | -1.69E-46 |
| MS.gene050724 | MS.gene08428 | 0.808069 | 3.71E-50 | -1.69E-46 |
| MS.gene050752 | MS.gene08428 | 0.804908 | 1.72E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene08428 | 0.825606 | 4.28E-54 | -1.69E-46 |
| MS.gene050808 | MS.gene08428 | 0.803902 | 2.79E-49 | -1.69E-46 |
| MS.gene050815 | MS.gene08428 | 0.875854 | 2.22E-68 | -1.69E-46 |
| MS.gene050838 | MS.gene08428 | 0.847984 | 8.18E-60 | -1.69E-46 |
| MS.gene051003 | MS.gene08428 | 0.813059 | 3.10E-51 | -1.69E-46 |
| MS.gene051063 | MS.gene08428 | 0.818308 | 2.10E-52 | -1.69E-46 |
| MS.gene051241 | MS.gene08428 | 0.801846 | 7.43E-49 | -1.69E-46 |
| MS.gene051243 | MS.gene08428 | 0.83682 | 7.48E-57 | -1.69E-46 |
| MS.gene051245 | MS.gene08428 | 0.836312 | 1.01E-56 | -1.69E-46 |
| MS.gene051328 | MS.gene08428 | 0.893898 | 4.09E-75 | -1.69E-46 |
| MS.gene05134 | MS.gene08428 | 0.867472 | 1.33E-65 | -1.69E-46 |
| MS.gene051577 | MS.gene08428 | 0.810069 | 1.38E-50 | -1.69E-46 |
| MS.gene05157 | MS.gene08428 | 0.805309 | 1.42E-49 | -1.69E-46 |
| MS.gene051633 | MS.gene08428 | 0.818191 | 2.23E-52 | -1.69E-46 |
| MS.gene051746 | MS.gene08428 | 0.849503 | 3.10E-60 | -1.69E-46 |
| MS.gene051748 | MS.gene08428 | 0.830693 | 2.54E-55 | -1.69E-46 |
| MS.gene051757 | MS.gene08428 | 0.843087 | 1.74E-58 | -1.69E-46 |
| MS.gene051850 | MS.gene08428 | 0.809932 | 1.48E-50 | -1.69E-46 |
| MS.gene05188 | MS.gene08428 | 0.801026 | 1.09E-48 | -1.69E-46 |
| MS.gene051903 | MS.gene08428 | 0.827817 | 1.27E-54 | -1.69E-46 |
| MS.gene052012 | MS.gene08428 | 0.806318 | 8.72E-50 | -1.69E-46 |
| MS.gene052133 | MS.gene08428 | 0.846558 | 2.01E-59 | -1.69E-46 |
| MS.gene052286 | MS.gene08428 | 0.831012 | 2.12E-55 | -1.69E-46 |
| MS.gene052302 | MS.gene08428 | 0.804767 | 1.84E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene08428 | 0.806505 | 7.96E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene08428 | 0.809878 | 1.52E-50 | -1.69E-46 |
| MS.gene052665 | MS.gene08428 | 0.810901 | 9.15E-51 | -1.69E-46 |
| MS.gene052856 | MS.gene08428 | 0.809191 | 2.14E-50 | -1.69E-46 |
| MS.gene052879 | MS.gene08428 | 0.843472 | 1.37E-58 | -1.69E-46 |
| MS.gene052880 | MS.gene08428 | 0.825201 | 5.33E-54 | -1.69E-46 |
| MS.gene052947 | MS.gene08428 | 0.864776 | 9.53E-65 | -1.69E-46 |
| MS.gene053008 | MS.gene08428 | 0.832716 | 8.06E-56 | -1.69E-46 |
| MS.gene053109 | MS.gene08428 | 0.801401 | 9.16E-49 | -1.69E-46 |
| MS.gene053581 | MS.gene08428 | 0.812254 | 4.65E-51 | -1.69E-46 |
| MS.gene053590 | MS.gene08428 | 0.816916 | 4.32E-52 | -1.69E-46 |
| MS.gene054403 | MS.gene08428 | 0.801848 | 7.42E-49 | -1.69E-46 |
| MS.gene054472 | MS.gene08428 | 0.800609 | 1.33E-48 | -1.69E-46 |
| MS.gene05462 | MS.gene08428 | 0.81887 | 1.56E-52 | -1.69E-46 |
| MS.gene055162 | MS.gene08428 | 0.86927 | 3.51E-66 | -1.69E-46 |
| MS.gene055466 | MS.gene08428 | 0.827747 | 1.32E-54 | -1.69E-46 |
| MS.gene055468 | MS.gene08428 | 0.837691 | 4.48E-57 | -1.69E-46 |
| MS.gene055512 | MS.gene08428 | 0.828061 | 1.11E-54 | -1.69E-46 |
| MS.gene055580 | MS.gene08428 | -0.807999 | 3.84E-50 | -1.69E-46 |
| MS.gene055608 | MS.gene08428 | 0.808201 | 3.48E-50 | -1.69E-46 |
| MS.gene055808 | MS.gene08428 | 0.802719 | 4.91E-49 | -1.69E-46 |
| MS.gene055912 | MS.gene08428 | 0.817327 | 3.49E-52 | -1.69E-46 |
| MS.gene056043 | MS.gene08428 | -0.817405 | 3.35E-52 | -1.69E-46 |
| MS.gene056069 | MS.gene08428 | -0.807414 | 5.11E-50 | -1.69E-46 |
| MS.gene056215 | MS.gene08428 | 0.817778 | 2.76E-52 | -1.69E-46 |
| MS.gene056432 | MS.gene08428 | 0.825531 | 4.46E-54 | -1.69E-46 |
| MS.gene056580 | MS.gene08428 | 0.830227 | 3.31E-55 | -1.69E-46 |
| MS.gene056601 | MS.gene08428 | 0.83158 | 1.54E-55 | -1.69E-46 |
| MS.gene056631 | MS.gene08428 | 0.877298 | 7.03E-69 | -1.69E-46 |
| MS.gene056760 | MS.gene08428 | 0.850781 | 1.36E-60 | -1.69E-46 |
| MS.gene057057 | MS.gene08428 | 0.862714 | 4.17E-64 | -1.69E-46 |
| MS.gene057094 | MS.gene08428 | 0.816085 | 6.63E-52 | -1.69E-46 |
| MS.gene057140 | MS.gene08428 | 0.870069 | 1.93E-66 | -1.69E-46 |
| MS.gene057234 | MS.gene08428 | 0.86298 | 3.45E-64 | -1.69E-46 |
| MS.gene057693 | MS.gene08428 | 0.821238 | 4.49E-53 | -1.69E-46 |
| MS.gene057694 | MS.gene08428 | 0.807983 | 3.87E-50 | -1.69E-46 |
| MS.gene057705 | MS.gene08428 | 0.850105 | 2.11E-60 | -1.69E-46 |
| MS.gene05795 | MS.gene08428 | 0.812326 | 4.49E-51 | -1.69E-46 |
| MS.gene058054 | MS.gene08428 | 0.81402 | 1.91E-51 | -1.69E-46 |
| MS.gene058055 | MS.gene08428 | 0.855701 | 5.29E-62 | -1.69E-46 |
| MS.gene058084 | MS.gene08428 | 0.861315 | 1.12E-63 | -1.69E-46 |
| MS.gene058238 | MS.gene08428 | 0.845795 | 3.25E-59 | -1.69E-46 |
| MS.gene058798 | MS.gene08428 | 0.802409 | 5.69E-49 | -1.69E-46 |
| MS.gene058852 | MS.gene08428 | 0.800893 | 1.16E-48 | -1.69E-46 |
| MS.gene058906 | MS.gene08428 | 0.910785 | 1.27E-82 | -1.69E-46 |
| MS.gene059151 | MS.gene08428 | 0.838346 | 3.04E-57 | -1.69E-46 |
| MS.gene059317 | MS.gene08428 | 0.81804 | 2.41E-52 | -1.69E-46 |
| MS.gene05978 | MS.gene08428 | 0.825709 | 4.04E-54 | -1.69E-46 |
| MS.gene059842 | MS.gene08428 | 0.812191 | 4.80E-51 | -1.69E-46 |
| MS.gene059898 | MS.gene08428 | 0.830643 | 2.62E-55 | -1.69E-46 |
| MS.gene059937 | MS.gene08428 | 0.82157 | 3.76E-53 | -1.69E-46 |
| MS.gene06001 | MS.gene08428 | 0.874679 | 5.59E-68 | -1.69E-46 |
| MS.gene060165 | MS.gene08428 | 0.808446 | 3.08E-50 | -1.69E-46 |
| MS.gene060194 | MS.gene08428 | 0.803736 | 3.02E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene08428 | 0.862666 | 4.31E-64 | -1.69E-46 |
| MS.gene060463 | MS.gene08428 | 0.800281 | 1.55E-48 | -1.69E-46 |
| MS.gene060465 | MS.gene08428 | 0.80583 | 1.10E-49 | -1.69E-46 |
| MS.gene060586 | MS.gene08428 | 0.826153 | 3.17E-54 | -1.69E-46 |
| MS.gene060587 | MS.gene08428 | 0.821246 | 4.47E-53 | -1.69E-46 |
| MS.gene060619 | MS.gene08428 | 0.812877 | 3.40E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene08428 | 0.801254 | 9.82E-49 | -1.69E-46 |
| MS.gene060917 | MS.gene08428 | 0.831842 | 1.33E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08428.t1 | MTR_8g080630 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 3.22e-67 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08428.t1 | AT1G67350 | 73.810 | 84 | 22 | 0 | 1 | 84 | 1 | 84 | 1.33e-45 | 142 |
| MS.gene08428.t1 | AT1G67350 | 73.810 | 84 | 22 | 0 | 1 | 84 | 1 | 84 | 1.33e-45 | 142 |
Find 30 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTACCCTATGCGACCTTA+TGG | 0.310546 | 4.2:+14318312 | MS.gene08428:CDS |
| AATCTACGATCCCTTTCATT+TGG | 0.347988 | 4.2:-14318234 | None:intergenic |
| CTATGCGACCTTATGGGTTC+TGG | 0.350279 | 4.2:+14318319 | MS.gene08428:CDS |
| AGTGAAAATGGCTTTCATAA+TGG | 0.354869 | 4.2:+14318173 | None:intergenic |
| ATGAAAGGGATCGTAGATTC+AGG | 0.366060 | 4.2:+14318238 | MS.gene08428:CDS |
| AATGGAATTCGCTGAGAATC+TGG | 0.378487 | 4.2:+14318191 | MS.gene08428:CDS |
| GTTACCCTATGCGACCTTAT+GGG | 0.431260 | 4.2:+14318313 | MS.gene08428:CDS |
| CCTCATTTCGACGACGAGGC+AGG | 0.443390 | 4.2:-14318438 | None:intergenic |
| GAATCTGGTTCTGAAATTGA+TGG | 0.457082 | 4.2:+14318206 | MS.gene08428:CDS |
| CCCAAGCAAGCTGAGAGTTA+TGG | 0.460911 | 4.2:-14318353 | None:intergenic |
| GCCATAACTCTCAGCTTGCT+TGG | 0.460971 | 4.2:+14318352 | MS.gene08428:CDS |
| CCATAACTCTCAGCTTGCTT+GGG | 0.463504 | 4.2:+14318353 | MS.gene08428:CDS |
| GCTCAGATTAGTCAAGTTGC+CGG | 0.484929 | 4.2:+14318378 | MS.gene08428:CDS |
| TGTGAAAGGATTTCATCATA+AGG | 0.496680 | 4.2:-14318411 | None:intergenic |
| CCTGCCTCGTCGTCGAAATG+AGG | 0.497822 | 4.2:+14318438 | MS.gene08428:CDS |
| CTCATTTCGACGACGAGGCA+GGG | 0.505092 | 4.2:-14318437 | None:intergenic |
| TCAAATGTCCAGAACCCATA+AGG | 0.513007 | 4.2:-14318327 | None:intergenic |
| TAGTCAAGTTGCCGGTCGTA+GGG | 0.521121 | 4.2:+14318386 | MS.gene08428:CDS |
| GATGGAAGATCCAAATGAAA+GGG | 0.524216 | 4.2:+14318224 | MS.gene08428:CDS |
| TGATGGAAGATCCAAATGAA+AGG | 0.535018 | 4.2:+14318223 | MS.gene08428:CDS |
| GGATAGATGCGCGAAGACGA+AGG | 0.541002 | 4.2:+14318281 | MS.gene08428:CDS |
| TGAAAGGGATCGTAGATTCA+GGG | 0.568833 | 4.2:+14318239 | MS.gene08428:CDS |
| CAGAACCCATAAGGTCGCAT+AGG | 0.571457 | 4.2:-14318318 | None:intergenic |
| GCGCGAAGACGAAGGAGATG+TGG | 0.571625 | 4.2:+14318289 | MS.gene08428:CDS |
| TTAGTCAAGTTGCCGGTCGT+AGG | 0.572671 | 4.2:+14318385 | MS.gene08428:CDS |
| ATCATAAGGATCCCTACGAC+CGG | 0.573881 | 4.2:-14318397 | None:intergenic |
| AGAACCCATAAGGTCGCATA+GGG | 0.582926 | 4.2:-14318317 | None:intergenic |
| ACGAGGCAGGGTAATGTGAA+AGG | 0.598363 | 4.2:-14318425 | None:intergenic |
| GGAACATGTGTATAAGACGA+AGG | 0.686694 | 4.2:+14318260 | MS.gene08428:CDS |
| AAAACCTCATTTCGACGACG+AGG | 0.717022 | 4.2:-14318442 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGTGAAAGGATTTCATCATA+AGG | - | chr4.2:14318414-14318433 | None:intergenic | 30.0% | |
| AATCTACGATCCCTTTCATT+TGG | - | chr4.2:14318237-14318256 | None:intergenic | 35.0% | |
| GATGGAAGATCCAAATGAAA+GGG | + | chr4.2:14318224-14318243 | MS.gene08428:CDS | 35.0% | |
| TGATGGAAGATCCAAATGAA+AGG | + | chr4.2:14318223-14318242 | MS.gene08428:CDS | 35.0% | |
| !! | GAATCTGGTTCTGAAATTGA+TGG | + | chr4.2:14318206-14318225 | MS.gene08428:CDS | 35.0% |
| AATGGAATTCGCTGAGAATC+TGG | + | chr4.2:14318191-14318210 | MS.gene08428:CDS | 40.0% | |
| ATGAAAGGGATCGTAGATTC+AGG | + | chr4.2:14318238-14318257 | MS.gene08428:CDS | 40.0% | |
| GGAACATGTGTATAAGACGA+AGG | + | chr4.2:14318260-14318279 | MS.gene08428:CDS | 40.0% | |
| TCAAATGTCCAGAACCCATA+AGG | - | chr4.2:14318330-14318349 | None:intergenic | 40.0% | |
| TGAAAGGGATCGTAGATTCA+GGG | + | chr4.2:14318239-14318258 | MS.gene08428:CDS | 40.0% | |
| AGAACCCATAAGGTCGCATA+GGG | - | chr4.2:14318320-14318339 | None:intergenic | 45.0% | |
| AGTTACCCTATGCGACCTTA+TGG | + | chr4.2:14318312-14318331 | MS.gene08428:CDS | 45.0% | |
| ATCATAAGGATCCCTACGAC+CGG | - | chr4.2:14318400-14318419 | None:intergenic | 45.0% | |
| GTTACCCTATGCGACCTTAT+GGG | + | chr4.2:14318313-14318332 | MS.gene08428:CDS | 45.0% | |
| ! | CCATAACTCTCAGCTTGCTT+GGG | + | chr4.2:14318353-14318372 | MS.gene08428:CDS | 45.0% |
| ! | GCTCAGATTAGTCAAGTTGC+CGG | + | chr4.2:14318378-14318397 | MS.gene08428:CDS | 45.0% |
| ACGAGGCAGGGTAATGTGAA+AGG | - | chr4.2:14318428-14318447 | None:intergenic | 50.0% | |
| CAGAACCCATAAGGTCGCAT+AGG | - | chr4.2:14318321-14318340 | None:intergenic | 50.0% | |
| CCCAAGCAAGCTGAGAGTTA+TGG | - | chr4.2:14318356-14318375 | None:intergenic | 50.0% | |
| CTATGCGACCTTATGGGTTC+TGG | + | chr4.2:14318319-14318338 | MS.gene08428:CDS | 50.0% | |
| ! | GCCATAACTCTCAGCTTGCT+TGG | + | chr4.2:14318352-14318371 | MS.gene08428:CDS | 50.0% |
| ! | TAGTCAAGTTGCCGGTCGTA+GGG | + | chr4.2:14318386-14318405 | MS.gene08428:CDS | 50.0% |
| ! | TTAGTCAAGTTGCCGGTCGT+AGG | + | chr4.2:14318385-14318404 | MS.gene08428:CDS | 50.0% |
| GGATAGATGCGCGAAGACGA+AGG | + | chr4.2:14318281-14318300 | MS.gene08428:CDS | 55.0% | |
| GCGCGAAGACGAAGGAGATG+TGG | + | chr4.2:14318289-14318308 | MS.gene08428:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 14318180 | 14318458 | 14318180 | ID=MS.gene08428 |
| chr4.2 | mRNA | 14318180 | 14318458 | 14318180 | ID=MS.gene08428.t1;Parent=MS.gene08428 |
| chr4.2 | exon | 14318180 | 14318458 | 14318180 | ID=MS.gene08428.t1.exon1;Parent=MS.gene08428.t1 |
| chr4.2 | CDS | 14318180 | 14318458 | 14318180 | ID=cds.MS.gene08428.t1;Parent=MS.gene08428.t1 |
| Gene Sequence |
| Protein sequence |
