AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068004


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068004.t1 MTR_4g093150 97.368 152 4 0 1 152 62 213 1.54e-104 298
MS.gene068004.t1 MTR_2g009770 57.463 134 54 2 7 139 128 259 8.61e-35 122
MS.gene068004.t1 MTR_1g054835 35.780 109 70 0 6 114 56 164 3.14e-13 66.2
MS.gene068004.t1 MTR_1g070350 37.234 94 59 0 15 108 533 626 6.72e-12 62.4
MS.gene068004.t1 MTR_5g078300 38.750 80 49 0 7 86 612 691 7.72e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068004.t1 AT5G16720 37.000 100 63 0 15 114 360 459 4.16e-15 71.6
MS.gene068004.t1 AT1G18265 52.273 88 42 0 5 92 183 270 1.63e-14 68.9
MS.gene068004.t1 AT1G70750 36.792 106 64 1 12 114 410 515 2.54e-14 69.7
MS.gene068004.t1 AT1G74830 37.500 96 60 0 15 110 305 400 2.89e-14 69.3
MS.gene068004.t1 AT5G06560 35.000 100 65 0 15 114 74 173 1.34e-12 64.3

Find 34 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAACTCAAGCAATATCT+TGG 0.374861 4.3:-21062235 MS.gene068004:CDS
TGATGAAGTGATCGAGTCTT+TGG 0.389665 4.3:-21062323 MS.gene068004:CDS
GAAAGCTAATGCGGCGTTTG+CGG 0.398717 4.3:-21062488 MS.gene068004:CDS
AAGAACTCAAGCAATATCTT+GGG 0.416257 4.3:-21062234 MS.gene068004:CDS
GTGTGTTGGAAGAGCAGTTG+AGG 0.428235 4.3:-21062267 MS.gene068004:CDS
AAGCAATATCTTGGGGAGGA+TGG 0.455312 4.3:-21062226 MS.gene068004:CDS
AGGCAATGGCGATGATTCTC+AGG 0.455510 4.3:-21062423 MS.gene068004:CDS
GGCTTCTTCATCTGCGGAAG+AGG 0.462933 4.3:-21062443 MS.gene068004:CDS
GTAGTTAGCTCTTCTGAAAA+CGG 0.463530 4.3:-21062139 MS.gene068004:CDS
GGCGTTTGCGGAGCTTGATA+AGG 0.464641 4.3:-21062476 MS.gene068004:CDS
TCTCGAGGCATGAATCTCAT+AGG 0.486643 4.3:-21062291 MS.gene068004:CDS
TGTGTTGGAAGAGCAGTTGA+GGG 0.487289 4.3:-21062266 MS.gene068004:CDS
AGCAATATCTTGGGGAGGAT+GGG 0.488013 4.3:-21062225 MS.gene068004:CDS
AAGCTGATGTTAGTAGAGAT+AGG 0.499821 4.3:-21062189 MS.gene068004:CDS
TTCATCTGCGGAAGAGGCAA+TGG 0.519311 4.3:-21062437 MS.gene068004:CDS
TACTAACATCAGCTTCAAGT+TGG 0.521972 4.3:+21062197 None:intergenic
CACAACCTTGGAAACTTACA+AGG 0.522214 4.3:+21062108 None:intergenic
AGCGAATCAATTCCGCAGAA+TGG 0.525489 4.3:-21062368 MS.gene068004:CDS
TCAAACACCTCATCCACATC+AGG 0.540118 4.3:+21062550 None:intergenic
AAGTGATCGAGTCTTTGGAA+TGG 0.572333 4.3:-21062318 MS.gene068004:CDS
TGAATCTCATAGGAGTGTGT+TGG 0.573968 4.3:-21062281 MS.gene068004:CDS
TTGCGGAGCTTGATAAGGAA+CGG 0.610513 4.3:-21062471 MS.gene068004:CDS
GCAGGTTCCTGATGTGGATG+AGG 0.610761 4.3:-21062557 MS.gene068004:CDS
CTTTGGAATGGACGATCTCG+AGG 0.612708 4.3:-21062306 MS.gene068004:CDS
AGTTGATAAAGATTGAGCGC+AGG 0.614343 4.3:-21062510 MS.gene068004:CDS
ACCTTGGAAACTTACAAGGT+CGG 0.615530 4.3:+21062112 None:intergenic
ACTTCTGTTCAGCCATTCTG+CGG 0.620830 4.3:+21062356 None:intergenic
TGAAATGCAGGTTCCTGATG+TGG 0.621860 4.3:-21062563 None:intergenic
GCTTCAGAGTGAGAAGAGCT+CGG 0.636966 4.3:-21062401 MS.gene068004:CDS
GCTTGATAAGGAACGGACAG+CGG 0.649607 4.3:-21062464 MS.gene068004:CDS
TGAGCGCAGGAAAGCTAATG+CGG 0.653423 4.3:-21062497 MS.gene068004:CDS
AGAACTCAAGCAATATCTTG+GGG 0.655002 4.3:-21062233 MS.gene068004:CDS
ACTCAAGCAATATCTTGGGG+AGG 0.657753 4.3:-21062230 MS.gene068004:CDS
GACAGCGGCTTCTTCATCTG+CGG 0.673445 4.3:-21062449 MS.gene068004:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGAACTCAAGCAATATCTT+GGG - chr4.3:21062448-21062467 MS.gene068004:CDS 30.0%
AAGCTGATGTTAGTAGAGAT+AGG - chr4.3:21062493-21062512 MS.gene068004:CDS 35.0%
AGAACTCAAGCAATATCTTG+GGG - chr4.3:21062449-21062468 MS.gene068004:CDS 35.0%
GAAGAACTCAAGCAATATCT+TGG - chr4.3:21062447-21062466 MS.gene068004:CDS 35.0%
TACTAACATCAGCTTCAAGT+TGG + chr4.3:21062488-21062507 None:intergenic 35.0%
! GTAGTTAGCTCTTCTGAAAA+CGG - chr4.3:21062543-21062562 MS.gene068004:CDS 35.0%
TGATGAAGTGATCGAGTCTT+TGG - chr4.3:21062359-21062378 MS.gene068004:CDS 40.0%
! AAGTGATCGAGTCTTTGGAA+TGG - chr4.3:21062364-21062383 MS.gene068004:CDS 40.0%
! TGAATCTCATAGGAGTGTGT+TGG - chr4.3:21062401-21062420 MS.gene068004:CDS 40.0%
!! AGTTGATAAAGATTGAGCGC+AGG - chr4.3:21062172-21062191 MS.gene068004:CDS 40.0%
AAGCAATATCTTGGGGAGGA+TGG - chr4.3:21062456-21062475 MS.gene068004:CDS 45.0%
ACTCAAGCAATATCTTGGGG+AGG - chr4.3:21062452-21062471 MS.gene068004:CDS 45.0%
ACTTCTGTTCAGCCATTCTG+CGG + chr4.3:21062329-21062348 None:intergenic 45.0%
AGCAATATCTTGGGGAGGAT+GGG - chr4.3:21062457-21062476 MS.gene068004:CDS 45.0%
AGCGAATCAATTCCGCAGAA+TGG - chr4.3:21062314-21062333 MS.gene068004:CDS 45.0%
TCAAACACCTCATCCACATC+AGG + chr4.3:21062135-21062154 None:intergenic 45.0%
TCTCGAGGCATGAATCTCAT+AGG - chr4.3:21062391-21062410 MS.gene068004:CDS 45.0%
TGTGTTGGAAGAGCAGTTGA+GGG - chr4.3:21062416-21062435 MS.gene068004:CDS 45.0%
TTGCGGAGCTTGATAAGGAA+CGG - chr4.3:21062211-21062230 MS.gene068004:CDS 45.0%
AGGCAATGGCGATGATTCTC+AGG - chr4.3:21062259-21062278 MS.gene068004:CDS 50.0%
GAAAGCTAATGCGGCGTTTG+CGG - chr4.3:21062194-21062213 MS.gene068004:CDS 50.0%
GCTTCAGAGTGAGAAGAGCT+CGG - chr4.3:21062281-21062300 MS.gene068004:CDS 50.0%
GCTTGATAAGGAACGGACAG+CGG - chr4.3:21062218-21062237 MS.gene068004:CDS 50.0%
GTGTGTTGGAAGAGCAGTTG+AGG - chr4.3:21062415-21062434 MS.gene068004:CDS 50.0%
TGAGCGCAGGAAAGCTAATG+CGG - chr4.3:21062185-21062204 MS.gene068004:CDS 50.0%
TTCATCTGCGGAAGAGGCAA+TGG - chr4.3:21062245-21062264 MS.gene068004:CDS 50.0%
!! CTTTGGAATGGACGATCTCG+AGG - chr4.3:21062376-21062395 MS.gene068004:CDS 50.0%
GACAGCGGCTTCTTCATCTG+CGG - chr4.3:21062233-21062252 MS.gene068004:CDS 55.0%
GCAGGTTCCTGATGTGGATG+AGG - chr4.3:21062125-21062144 MS.gene068004:CDS 55.0%
GGCGTTTGCGGAGCTTGATA+AGG - chr4.3:21062206-21062225 MS.gene068004:CDS 55.0%
GGCTTCTTCATCTGCGGAAG+AGG - chr4.3:21062239-21062258 MS.gene068004:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 21062123 21062581 21062123 ID=MS.gene068004
chr4.3 mRNA 21062123 21062581 21062123 ID=MS.gene068004.t1;Parent=MS.gene068004
chr4.3 exon 21062123 21062581 21062123 ID=MS.gene068004.t1.exon1;Parent=MS.gene068004.t1
chr4.3 CDS 21062123 21062581 21062123 ID=cds.MS.gene068004.t1;Parent=MS.gene068004.t1
Gene Sequence

>MS.gene068004

ATGCAGGTTCCTGATGTGGATGAGGTGTTTGATGTAATCACTCTGAGAAAGTTGATAAAGATTGAGCGCAGGAAAGCTAATGCGGCGTTTGCGGAGCTTGATAAGGAACGGACAGCGGCTTCTTCATCTGCGGAAGAGGCAATGGCGATGATTCTCAGGCTTCAGAGTGAGAAGAGCTCGGCTGAAATTCAAGCGAATCAATTCCGCAGAATGGCTGAACAGAAGTTAGAGTATGATGATGAAGTGATCGAGTCTTTGGAATGGACGATCTCGAGGCATGAATCTCATAGGAGTGTGTTGGAAGAGCAGTTGAGGGTTTATAGAGAAGAACTCAAGCAATATCTTGGGGAGGATGGGATAAACCAACTTGAAGCTGATGTTAGTAGAGATAGGAGTTTTGAGAATGAAGCAGTTGATTCTGTAGTTAGCTCTTCTGAAAACGGATCACCGACCTTGTAA

Protein sequence

>MS.gene068004.t1

MQVPDVDEVFDVITLRKLIKIERRKANAAFAELDKERTAASSSAEEAMAMILRLQSEKSSAEIQANQFRRMAEQKLEYDDEVIESLEWTISRHESHRSVLEEQLRVYREELKQYLGEDGINQLEADVSRDRSFENEAVDSVVSSSENGSPTL