AlfalfaGEDB | MS.gene068006

M. sativa cultivar XinJiangDaYe / MS.gene068006

KEGG: -
GO: -
PFAM: PF04576.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068006.t1	RHN62642.1	97.4	151	4	0	1	151	289	439	4.30E-68	267.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068006.t1	Q9LM24	53.7	82	38	0	11	92	189	270	3.6e-15	82.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068006.t1	G7JU33	97.4	151	4	0	1	151	62	212	3.1e-68	267.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene068006	0.803063	4.17E-49	-1.69E-46
MS.gene049254	MS.gene068006	0.812199	4.78E-51	-1.69E-46
MS.gene049271	MS.gene068006	0.807805	4.22E-50	-1.69E-46
MS.gene049341	MS.gene068006	0.815865	7.43E-52	-1.69E-46
MS.gene049350	MS.gene068006	0.819152	1.35E-52	-1.69E-46
MS.gene049404	MS.gene068006	0.830504	2.83E-55	-1.69E-46
MS.gene049711	MS.gene068006	0.830161	3.43E-55	-1.69E-46
MS.gene049821	MS.gene068006	0.840483	8.46E-58	-1.69E-46
MS.gene049824	MS.gene068006	0.831856	1.32E-55	-1.69E-46
MS.gene049825	MS.gene068006	0.829601	4.70E-55	-1.69E-46
MS.gene049850	MS.gene068006	0.830394	3.01E-55	-1.69E-46
MS.gene049873	MS.gene068006	0.836345	9.88E-57	-1.69E-46
MS.gene050020	MS.gene068006	0.832037	1.19E-55	-1.69E-46
MS.gene05016	MS.gene068006	0.822798	1.95E-53	-1.69E-46
MS.gene050201	MS.gene068006	0.807379	5.20E-50	-1.69E-46
MS.gene050273	MS.gene068006	0.814763	1.31E-51	-1.69E-46
MS.gene050559	MS.gene068006	0.819596	1.07E-52	-1.69E-46
MS.gene050561	MS.gene068006	0.815059	1.12E-51	-1.69E-46
MS.gene050562	MS.gene068006	0.804369	2.23E-49	-1.69E-46
MS.gene050807	MS.gene068006	0.848705	5.17E-60	-1.69E-46
MS.gene050808	MS.gene068006	0.802734	4.87E-49	-1.69E-46
MS.gene050901	MS.gene068006	0.804111	2.53E-49	-1.69E-46
MS.gene051198	MS.gene068006	0.806238	9.07E-50	-1.69E-46
MS.gene051247	MS.gene068006	0.839798	1.28E-57	-1.69E-46
MS.gene051279	MS.gene068006	0.835288	1.83E-56	-1.69E-46
MS.gene051328	MS.gene068006	0.80715	5.82E-50	-1.69E-46
MS.gene05157	MS.gene068006	0.808709	2.71E-50	-1.69E-46
MS.gene051628	MS.gene068006	0.800333	1.51E-48	-1.69E-46
MS.gene051700	MS.gene068006	0.810923	9.06E-51	-1.69E-46
MS.gene051719	MS.gene068006	0.872507	3.02E-67	-1.69E-46
MS.gene051720	MS.gene068006	0.875942	2.07E-68	-1.69E-46
MS.gene051744	MS.gene068006	0.831901	1.28E-55	-1.69E-46
MS.gene051957	MS.gene068006	0.817641	2.97E-52	-1.69E-46
MS.gene052133	MS.gene068006	0.838358	3.02E-57	-1.69E-46
MS.gene052239	MS.gene068006	-0.807196	5.69E-50	-1.69E-46
MS.gene052463	MS.gene068006	0.804031	2.63E-49	-1.69E-46
MS.gene052516	MS.gene068006	0.848408	6.25E-60	-1.69E-46
MS.gene052519	MS.gene068006	0.837799	4.20E-57	-1.69E-46
MS.gene052582	MS.gene068006	0.822443	2.36E-53	-1.69E-46
MS.gene052584	MS.gene068006	0.826139	3.20E-54	-1.69E-46
MS.gene052611	MS.gene068006	0.815539	8.78E-52	-1.69E-46
MS.gene052665	MS.gene068006	0.834234	3.37E-56	-1.69E-46
MS.gene052773	MS.gene068006	0.820207	7.75E-53	-1.69E-46
MS.gene052863	MS.gene068006	0.802466	5.54E-49	-1.69E-46
MS.gene052896	MS.gene068006	0.80208	6.65E-49	-1.69E-46
MS.gene053079	MS.gene068006	0.800213	1.60E-48	-1.69E-46
MS.gene053144	MS.gene068006	0.816252	6.09E-52	-1.69E-46
MS.gene053221	MS.gene068006	0.820474	6.73E-53	-1.69E-46
MS.gene053226	MS.gene068006	0.848909	4.54E-60	-1.69E-46
MS.gene053563	MS.gene068006	0.804987	1.66E-49	-1.69E-46
MS.gene053581	MS.gene068006	0.822624	2.14E-53	-1.69E-46
MS.gene053597	MS.gene068006	0.920297	1.53E-87	-1.69E-46
MS.gene053599	MS.gene068006	0.959844	6.84E-118	-1.69E-46
MS.gene05364	MS.gene068006	0.813129	2.99E-51	-1.69E-46
MS.gene053678	MS.gene068006	0.823601	1.27E-53	-1.69E-46
MS.gene053883	MS.gene068006	0.810157	1.33E-50	-1.69E-46
MS.gene053995	MS.gene068006	0.815867	7.42E-52	-1.69E-46
MS.gene05415	MS.gene068006	0.802524	5.38E-49	-1.69E-46
MS.gene054236	MS.gene068006	0.809122	2.21E-50	-1.69E-46
MS.gene054584	MS.gene068006	0.820991	5.12E-53	-1.69E-46
MS.gene054651	MS.gene068006	0.842723	2.17E-58	-1.69E-46
MS.gene054775	MS.gene068006	0.801579	8.42E-49	-1.69E-46
MS.gene055227	MS.gene068006	0.803251	3.81E-49	-1.69E-46
MS.gene055465	MS.gene068006	0.861699	8.54E-64	-1.69E-46
MS.gene055466	MS.gene068006	0.868491	6.28E-66	-1.69E-46
MS.gene055468	MS.gene068006	0.863699	2.07E-64	-1.69E-46
MS.gene055512	MS.gene068006	0.839725	1.33E-57	-1.69E-46
MS.gene055537	MS.gene068006	0.813141	2.97E-51	-1.69E-46
MS.gene055580	MS.gene068006	-0.824256	8.91E-54	-1.69E-46
MS.gene055778	MS.gene068006	0.823106	1.66E-53	-1.69E-46
MS.gene055818	MS.gene068006	0.816072	6.68E-52	-1.69E-46
MS.gene055890	MS.gene068006	0.80677	7.00E-50	-1.69E-46
MS.gene055891	MS.gene068006	0.822224	2.66E-53	-1.69E-46
MS.gene056005	MS.gene068006	0.807867	4.10E-50	-1.69E-46
MS.gene056043	MS.gene068006	-0.807531	4.83E-50	-1.69E-46
MS.gene05604	MS.gene068006	0.823071	1.69E-53	-1.69E-46
MS.gene056050	MS.gene068006	0.817227	3.68E-52	-1.69E-46
MS.gene056215	MS.gene068006	0.800448	1.43E-48	-1.69E-46
MS.gene056308	MS.gene068006	0.824071	9.84E-54	-1.69E-46
MS.gene056366	MS.gene068006	0.824471	7.93E-54	-1.69E-46
MS.gene056595	MS.gene068006	0.823673	1.22E-53	-1.69E-46
MS.gene056643	MS.gene068006	0.806531	7.86E-50	-1.69E-46
MS.gene056859	MS.gene068006	0.804088	2.55E-49	-1.69E-46
MS.gene057060	MS.gene068006	0.831182	1.93E-55	-1.69E-46
MS.gene057314	MS.gene068006	0.80378	2.96E-49	-1.69E-46
MS.gene057444	MS.gene068006	0.812167	4.86E-51	-1.69E-46
MS.gene057693	MS.gene068006	0.802747	4.85E-49	-1.69E-46
MS.gene057705	MS.gene068006	0.805098	1.57E-49	-1.69E-46
MS.gene057915	MS.gene068006	0.82345	1.38E-53	-1.69E-46
MS.gene058037	MS.gene068006	0.810248	1.27E-50	-1.69E-46
MS.gene058216	MS.gene068006	0.80759	4.69E-50	-1.69E-46
MS.gene058625	MS.gene068006	0.85059	1.54E-60	-1.69E-46
MS.gene058636	MS.gene068006	0.809384	1.94E-50	-1.69E-46
MS.gene058829	MS.gene068006	0.8112	7.89E-51	-1.69E-46
MS.gene058852	MS.gene068006	0.80253	5.37E-49	-1.69E-46
MS.gene059015	MS.gene068006	0.84209	3.20E-58	-1.69E-46
MS.gene05954	MS.gene068006	0.846958	1.57E-59	-1.69E-46
MS.gene059625	MS.gene068006	0.807842	4.15E-50	-1.69E-46
MS.gene059725	MS.gene068006	0.801509	8.71E-49	-1.69E-46
MS.gene05978	MS.gene068006	0.812182	4.82E-51	-1.69E-46
MS.gene060365	MS.gene068006	0.809831	1.56E-50	-1.69E-46
MS.gene060429	MS.gene068006	0.818898	1.54E-52	-1.69E-46
MS.gene060519	MS.gene068006	0.826588	2.50E-54	-1.69E-46
MS.gene060521	MS.gene068006	0.83417	3.50E-56	-1.69E-46
MS.gene060587	MS.gene068006	0.852581	4.20E-61	-1.69E-46
MS.gene060634	MS.gene068006	-0.807153	5.81E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068006.t1	MTR_4g093150	97.351	151	4	0	1	151	62	212	5.40e-104	296
MS.gene068006.t1	MTR_2g009770	57.463	134	54	2	7	139	128	259	9.56e-35	122
MS.gene068006.t1	MTR_1g054835	35.780	109	70	0	6	114	56	164	2.47e-13	66.6
MS.gene068006.t1	MTR_1g070350	37.234	94	59	0	15	108	533	626	6.04e-12	62.8
MS.gene068006.t1	MTR_5g078300	38.750	80	49	0	7	86	612	691	6.88e-12	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068006.t1	AT5G16720	37.000	100	63	0	15	114	360	459	3.88e-15	71.6
MS.gene068006.t1	AT1G18265	52.273	88	42	0	5	92	183	270	1.65e-14	68.9
MS.gene068006.t1	AT1G70750	36.792	106	64	1	12	114	410	515	2.21e-14	69.7
MS.gene068006.t1	AT1G74830	37.500	96	60	0	15	110	305	400	2.83e-14	69.3
MS.gene068006.t1	AT5G06560	35.000	100	65	0	15	114	74	173	1.30e-12	64.3

Find 33 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGAACTCAAGCAATATCT+TGG	0.374861	4.3:-21076443	MS.gene068006:CDS
TGATGAAGTGATCGAGTCTT+TGG	0.389665	4.3:-21076531	MS.gene068006:CDS
GAAAGCTAATGCGGCGTTTG+CGG	0.398717	4.3:-21076696	MS.gene068006:CDS
AAGAACTCAAGCAATATCTT+GGG	0.416257	4.3:-21076442	MS.gene068006:CDS
GTGTGTTGGAAGAGCAGTTG+AGG	0.428235	4.3:-21076475	MS.gene068006:CDS
AAGCAATATCTTGGGGAGGA+TGG	0.455312	4.3:-21076434	MS.gene068006:CDS
AGGCAATGGCGATGATTCTC+AGG	0.455510	4.3:-21076631	MS.gene068006:CDS
GGCTTCTTCATCTGCGGAAG+AGG	0.462933	4.3:-21076651	MS.gene068006:CDS
GTAGTTAGCTCTTCTGAAAA+CGG	0.463530	4.3:-21076347	MS.gene068006:CDS
GGCGTTTGCGGAGCTTGATA+AGG	0.464641	4.3:-21076684	MS.gene068006:CDS
TCTCGAGGCATGAATCTCAT+AGG	0.486643	4.3:-21076499	MS.gene068006:CDS
TGTGTTGGAAGAGCAGTTGA+GGG	0.487289	4.3:-21076474	MS.gene068006:CDS
AGCAATATCTTGGGGAGGAT+GGG	0.488013	4.3:-21076433	MS.gene068006:CDS
AAGCTGATGTTAGTAGAGAT+AGG	0.499821	4.3:-21076397	MS.gene068006:CDS
TTCATCTGCGGAAGAGGCAA+TGG	0.519311	4.3:-21076645	MS.gene068006:CDS
TACTAACATCAGCTTCAAGT+TGG	0.521972	4.3:+21076405	None:intergenic
AGCGAATCAATTCCGCAGAA+TGG	0.525489	4.3:-21076576	MS.gene068006:CDS
TCAAACACCTCATCCACATC+AGG	0.540118	4.3:+21076758	None:intergenic
AAGTGATCGAGTCTTTGGAA+TGG	0.572333	4.3:-21076526	MS.gene068006:CDS
TGAATCTCATAGGAGTGTGT+TGG	0.573968	4.3:-21076489	MS.gene068006:CDS
TTGCGGAGCTTGATAAGGAA+CGG	0.610513	4.3:-21076679	MS.gene068006:CDS
GCAGGTTCCTGATGTGGATG+AGG	0.610761	4.3:-21076765	MS.gene068006:CDS
CTTTGGAATGGACGATCTCG+AGG	0.612708	4.3:-21076514	MS.gene068006:CDS
AGTTGATAAAGATTGAGCGC+AGG	0.614343	4.3:-21076718	MS.gene068006:CDS
ACCTTGGAAACTTACAAGGT+CGG	0.615530	4.3:+21076320	None:intergenic
ACTTCTGTTCAGCCATTCTG+CGG	0.620830	4.3:+21076564	None:intergenic
TGAAATGCAGGTTCCTGATG+TGG	0.621860	4.3:-21076771	None:intergenic
GCTTCAGAGTGAGAAGAGCT+CGG	0.636966	4.3:-21076609	MS.gene068006:CDS
GCTTGATAAGGAACGGACAG+CGG	0.649607	4.3:-21076672	MS.gene068006:CDS
TGAGCGCAGGAAAGCTAATG+CGG	0.653423	4.3:-21076705	MS.gene068006:CDS
AGAACTCAAGCAATATCTTG+GGG	0.655002	4.3:-21076441	MS.gene068006:CDS
ACTCAAGCAATATCTTGGGG+AGG	0.657753	4.3:-21076438	MS.gene068006:CDS
GACAGCGGCTTCTTCATCTG+CGG	0.673445	4.3:-21076657	MS.gene068006:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGAACTCAAGCAATATCTT+GGG	-	chr4.3:21076660-21076679	MS.gene068006:CDS	30.0%
	AAGCTGATGTTAGTAGAGAT+AGG	-	chr4.3:21076705-21076724	MS.gene068006:CDS	35.0%
	AGAACTCAAGCAATATCTTG+GGG	-	chr4.3:21076661-21076680	MS.gene068006:CDS	35.0%
	GAAGAACTCAAGCAATATCT+TGG	-	chr4.3:21076659-21076678	MS.gene068006:CDS	35.0%
	TACTAACATCAGCTTCAAGT+TGG	+	chr4.3:21076700-21076719	None:intergenic	35.0%
!	GTAGTTAGCTCTTCTGAAAA+CGG	-	chr4.3:21076755-21076774	MS.gene068006:CDS	35.0%
	TGATGAAGTGATCGAGTCTT+TGG	-	chr4.3:21076571-21076590	MS.gene068006:CDS	40.0%
!	AAGTGATCGAGTCTTTGGAA+TGG	-	chr4.3:21076576-21076595	MS.gene068006:CDS	40.0%
!	TGAATCTCATAGGAGTGTGT+TGG	-	chr4.3:21076613-21076632	MS.gene068006:CDS	40.0%
!!	AGTTGATAAAGATTGAGCGC+AGG	-	chr4.3:21076384-21076403	MS.gene068006:CDS	40.0%
	AAGCAATATCTTGGGGAGGA+TGG	-	chr4.3:21076668-21076687	MS.gene068006:CDS	45.0%
	ACTCAAGCAATATCTTGGGG+AGG	-	chr4.3:21076664-21076683	MS.gene068006:CDS	45.0%
	ACTTCTGTTCAGCCATTCTG+CGG	+	chr4.3:21076541-21076560	None:intergenic	45.0%
	AGCAATATCTTGGGGAGGAT+GGG	-	chr4.3:21076669-21076688	MS.gene068006:CDS	45.0%
	AGCGAATCAATTCCGCAGAA+TGG	-	chr4.3:21076526-21076545	MS.gene068006:CDS	45.0%
	TCAAACACCTCATCCACATC+AGG	+	chr4.3:21076347-21076366	None:intergenic	45.0%
	TCTCGAGGCATGAATCTCAT+AGG	-	chr4.3:21076603-21076622	MS.gene068006:CDS	45.0%
	TGTGTTGGAAGAGCAGTTGA+GGG	-	chr4.3:21076628-21076647	MS.gene068006:CDS	45.0%
	TTGCGGAGCTTGATAAGGAA+CGG	-	chr4.3:21076423-21076442	MS.gene068006:CDS	45.0%
	AGGCAATGGCGATGATTCTC+AGG	-	chr4.3:21076471-21076490	MS.gene068006:CDS	50.0%
	GAAAGCTAATGCGGCGTTTG+CGG	-	chr4.3:21076406-21076425	MS.gene068006:CDS	50.0%
	GCTTCAGAGTGAGAAGAGCT+CGG	-	chr4.3:21076493-21076512	MS.gene068006:CDS	50.0%
	GCTTGATAAGGAACGGACAG+CGG	-	chr4.3:21076430-21076449	MS.gene068006:CDS	50.0%
	GTGTGTTGGAAGAGCAGTTG+AGG	-	chr4.3:21076627-21076646	MS.gene068006:CDS	50.0%
	TGAGCGCAGGAAAGCTAATG+CGG	-	chr4.3:21076397-21076416	MS.gene068006:CDS	50.0%
	TTCATCTGCGGAAGAGGCAA+TGG	-	chr4.3:21076457-21076476	MS.gene068006:CDS	50.0%
!!	CTTTGGAATGGACGATCTCG+AGG	-	chr4.3:21076588-21076607	MS.gene068006:CDS	50.0%
	GACAGCGGCTTCTTCATCTG+CGG	-	chr4.3:21076445-21076464	MS.gene068006:CDS	55.0%
	GCAGGTTCCTGATGTGGATG+AGG	-	chr4.3:21076337-21076356	MS.gene068006:CDS	55.0%
	GGCGTTTGCGGAGCTTGATA+AGG	-	chr4.3:21076418-21076437	MS.gene068006:CDS	55.0%
	GGCTTCTTCATCTGCGGAAG+AGG	-	chr4.3:21076451-21076470	MS.gene068006:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	21076335	21076789	21076335	ID=MS.gene068006
chr4.3	mRNA	21076335	21076789	21076335	ID=MS.gene068006.t1;Parent=MS.gene068006
chr4.3	exon	21076335	21076789	21076335	ID=MS.gene068006.t1.exon1;Parent=MS.gene068006.t1
chr4.3	CDS	21076335	21076789	21076335	ID=cds.MS.gene068006.t1;Parent=MS.gene068006.t1

Gene Sequence

>MS.gene068006

ATGCAGGTTCCTGATGTGGATGAGGTGTTTGATGTAATCACTCTGAGAAAGTTGATAAAGATTGAGCGCAGGAAAGCTAATGCGGCGTTTGCGGAGCTTGATAAGGAACGGACAGCGGCTTCTTCATCTGCGGAAGAGGCAATGGCGATGATTCTCAGGCTTCAGAGTGAGAAGAGCTCGGCTGAAATTCAAGCGAATCAATTCCGCAGAATGGCTGAACAGAAGTTAGAGTATGATGATGAAGTGATCGAGTCTTTGGAATGGACGATCTCGAGGCATGAATCTCATAGGAGTGTGTTGGAAGAGCAGTTGAGGGTTTATAGAGAAGAACTCAAGCAATATCTTGGGGAGGATGGGATAAACCAACTTGAAGCTGATGTTAGTAGAGATAGGAGTTTTGAGAATGAAGCAGTTGATTCTGTAGTTAGCTCTTCTGAAAACGGATCACCGACCTT

Protein sequence

>MS.gene068006.t1

MQVPDVDEVFDVITLRKLIKIERRKANAAFAELDKERTAASSSAEEAMAMILRLQSEKSSAEIQANQFRRMAEQKLEYDDEVIESLEWTISRHESHRSVLEEQLRVYREELKQYLGEDGINQLEADVSRDRSFENEAVDSVVSSSENGSPT