Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47689.t1 | RHN57948.1 | 57 | 200 | 60 | 3 | 1 | 200 | 1 | 174 | 4.20E-38 | 169.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47689.t1 | G7K4I5 | 57.0 | 200 | 60 | 3 | 1 | 200 | 1 | 174 | 3.1e-38 | 169.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene47689 | 0.825396 | 4.80E-54 | -1.69E-46 |
| MS.gene049159 | MS.gene47689 | 0.827549 | 1.47E-54 | -1.69E-46 |
| MS.gene049190 | MS.gene47689 | 0.816089 | 6.62E-52 | -1.69E-46 |
| MS.gene049341 | MS.gene47689 | 0.821931 | 3.11E-53 | -1.69E-46 |
| MS.gene049404 | MS.gene47689 | 0.830747 | 2.47E-55 | -1.69E-46 |
| MS.gene049435 | MS.gene47689 | 0.81253 | 4.05E-51 | -1.69E-46 |
| MS.gene049501 | MS.gene47689 | 0.807028 | 6.17E-50 | -1.69E-46 |
| MS.gene049821 | MS.gene47689 | 0.83257 | 8.77E-56 | -1.69E-46 |
| MS.gene049824 | MS.gene47689 | 0.811446 | 6.97E-51 | -1.69E-46 |
| MS.gene049850 | MS.gene47689 | 0.843197 | 1.62E-58 | -1.69E-46 |
| MS.gene049979 | MS.gene47689 | 0.810746 | 9.89E-51 | -1.69E-46 |
| MS.gene050020 | MS.gene47689 | 0.863122 | 3.12E-64 | -1.69E-46 |
| MS.gene050241 | MS.gene47689 | 0.802567 | 5.28E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene47689 | 0.862632 | 4.42E-64 | -1.69E-46 |
| MS.gene050808 | MS.gene47689 | 0.829732 | 4.37E-55 | -1.69E-46 |
| MS.gene050815 | MS.gene47689 | 0.810594 | 1.07E-50 | -1.69E-46 |
| MS.gene050901 | MS.gene47689 | 0.818672 | 1.74E-52 | -1.69E-46 |
| MS.gene050959 | MS.gene47689 | 0.801578 | 8.43E-49 | -1.69E-46 |
| MS.gene051102 | MS.gene47689 | 0.801992 | 6.93E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene47689 | 0.836743 | 7.83E-57 | -1.69E-46 |
| MS.gene051265 | MS.gene47689 | 0.805609 | 1.23E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene47689 | 0.837317 | 5.58E-57 | -1.69E-46 |
| MS.gene051328 | MS.gene47689 | 0.830431 | 2.95E-55 | -1.69E-46 |
| MS.gene051388 | MS.gene47689 | 0.801607 | 8.31E-49 | -1.69E-46 |
| MS.gene05157 | MS.gene47689 | 0.854691 | 1.04E-61 | -1.69E-46 |
| MS.gene051700 | MS.gene47689 | 0.818558 | 1.84E-52 | -1.69E-46 |
| MS.gene051719 | MS.gene47689 | 0.886417 | 3.48E-72 | -1.69E-46 |
| MS.gene051720 | MS.gene47689 | 0.884173 | 2.40E-71 | -1.69E-46 |
| MS.gene051744 | MS.gene47689 | 0.80887 | 2.50E-50 | -1.69E-46 |
| MS.gene052020 | MS.gene47689 | 0.83915 | 1.88E-57 | -1.69E-46 |
| MS.gene052133 | MS.gene47689 | 0.85297 | 3.25E-61 | -1.69E-46 |
| MS.gene052323 | MS.gene47689 | 0.807404 | 5.14E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene47689 | 0.85149 | 8.58E-61 | -1.69E-46 |
| MS.gene052519 | MS.gene47689 | 0.806922 | 6.50E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene47689 | 0.806084 | 9.76E-50 | -1.69E-46 |
| MS.gene052611 | MS.gene47689 | 0.81646 | 5.47E-52 | -1.69E-46 |
| MS.gene052665 | MS.gene47689 | 0.867675 | 1.15E-65 | -1.69E-46 |
| MS.gene052732 | MS.gene47689 | 0.836354 | 9.83E-57 | -1.69E-46 |
| MS.gene052896 | MS.gene47689 | 0.809841 | 1.55E-50 | -1.69E-46 |
| MS.gene053079 | MS.gene47689 | 0.817919 | 2.57E-52 | -1.69E-46 |
| MS.gene053144 | MS.gene47689 | 0.852314 | 5.01E-61 | -1.69E-46 |
| MS.gene053174 | MS.gene47689 | 0.822113 | 2.82E-53 | -1.69E-46 |
| MS.gene053221 | MS.gene47689 | 0.822046 | 2.92E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene47689 | 0.837684 | 4.49E-57 | -1.69E-46 |
| MS.gene053565 | MS.gene47689 | 0.803996 | 2.67E-49 | -1.69E-46 |
| MS.gene053581 | MS.gene47689 | 0.843702 | 1.19E-58 | -1.69E-46 |
| MS.gene053599 | MS.gene47689 | 0.829406 | 5.24E-55 | -1.69E-46 |
| MS.gene05364 | MS.gene47689 | 0.832273 | 1.04E-55 | -1.69E-46 |
| MS.gene054550 | MS.gene47689 | 0.822819 | 1.93E-53 | -1.69E-46 |
| MS.gene054584 | MS.gene47689 | 0.861315 | 1.12E-63 | -1.69E-46 |
| MS.gene05462 | MS.gene47689 | 0.81267 | 3.77E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene47689 | 0.812473 | 4.16E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene47689 | 0.804978 | 1.67E-49 | -1.69E-46 |
| MS.gene054801 | MS.gene47689 | 0.803481 | 3.42E-49 | -1.69E-46 |
| MS.gene055144 | MS.gene47689 | 0.811635 | 6.34E-51 | -1.69E-46 |
| MS.gene055357 | MS.gene47689 | 0.811691 | 6.17E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene47689 | 0.878499 | 2.67E-69 | -1.69E-46 |
| MS.gene055466 | MS.gene47689 | 0.877596 | 5.53E-69 | -1.69E-46 |
| MS.gene055468 | MS.gene47689 | 0.862885 | 3.69E-64 | -1.69E-46 |
| MS.gene055469 | MS.gene47689 | 0.842795 | 2.08E-58 | -1.69E-46 |
| MS.gene055512 | MS.gene47689 | 0.826205 | 3.08E-54 | -1.69E-46 |
| MS.gene055537 | MS.gene47689 | 0.817369 | 3.42E-52 | -1.69E-46 |
| MS.gene055580 | MS.gene47689 | -0.844976 | 5.41E-59 | -1.69E-46 |
| MS.gene055608 | MS.gene47689 | 0.812327 | 4.48E-51 | -1.69E-46 |
| MS.gene055890 | MS.gene47689 | 0.836504 | 9.00E-57 | -1.69E-46 |
| MS.gene055891 | MS.gene47689 | 0.831803 | 1.36E-55 | -1.69E-46 |
| MS.gene056005 | MS.gene47689 | 0.805784 | 1.13E-49 | -1.69E-46 |
| MS.gene056043 | MS.gene47689 | -0.827147 | 1.84E-54 | -1.69E-46 |
| MS.gene056173 | MS.gene47689 | 0.806928 | 6.48E-50 | -1.69E-46 |
| MS.gene056215 | MS.gene47689 | 0.807811 | 4.21E-50 | -1.69E-46 |
| MS.gene056308 | MS.gene47689 | 0.841362 | 4.98E-58 | -1.69E-46 |
| MS.gene056366 | MS.gene47689 | 0.820296 | 7.39E-53 | -1.69E-46 |
| MS.gene056507 | MS.gene47689 | 0.808858 | 2.52E-50 | -1.69E-46 |
| MS.gene056553 | MS.gene47689 | 0.831422 | 1.68E-55 | -1.69E-46 |
| MS.gene056595 | MS.gene47689 | 0.806297 | 8.81E-50 | -1.69E-46 |
| MS.gene056643 | MS.gene47689 | 0.816927 | 4.30E-52 | -1.69E-46 |
| MS.gene056859 | MS.gene47689 | 0.804274 | 2.34E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene47689 | 0.819728 | 9.97E-53 | -1.69E-46 |
| MS.gene057444 | MS.gene47689 | 0.834908 | 2.28E-56 | -1.69E-46 |
| MS.gene057446 | MS.gene47689 | 0.808846 | 2.53E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene47689 | 0.8044 | 2.20E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene47689 | 0.816548 | 5.22E-52 | -1.69E-46 |
| MS.gene057915 | MS.gene47689 | 0.822839 | 1.91E-53 | -1.69E-46 |
| MS.gene058010 | MS.gene47689 | 0.818195 | 2.22E-52 | -1.69E-46 |
| MS.gene058087 | MS.gene47689 | 0.80732 | 5.35E-50 | -1.69E-46 |
| MS.gene058339 | MS.gene47689 | 0.822414 | 2.40E-53 | -1.69E-46 |
| MS.gene058625 | MS.gene47689 | 0.84015 | 1.03E-57 | -1.69E-46 |
| MS.gene058636 | MS.gene47689 | 0.815083 | 1.11E-51 | -1.69E-46 |
| MS.gene058829 | MS.gene47689 | 0.807885 | 4.06E-50 | -1.69E-46 |
| MS.gene058852 | MS.gene47689 | 0.82298 | 1.77E-53 | -1.69E-46 |
| MS.gene059015 | MS.gene47689 | 0.82976 | 4.30E-55 | -1.69E-46 |
| MS.gene059570 | MS.gene47689 | 0.816565 | 5.18E-52 | -1.69E-46 |
| MS.gene059625 | MS.gene47689 | 0.821993 | 3.00E-53 | -1.69E-46 |
| MS.gene059725 | MS.gene47689 | 0.807055 | 6.09E-50 | -1.69E-46 |
| MS.gene05978 | MS.gene47689 | 0.810601 | 1.06E-50 | -1.69E-46 |
| MS.gene059842 | MS.gene47689 | 0.809619 | 1.73E-50 | -1.69E-46 |
| MS.gene059937 | MS.gene47689 | 0.819358 | 1.21E-52 | -1.69E-46 |
| MS.gene060194 | MS.gene47689 | 0.851296 | 9.74E-61 | -1.69E-46 |
| MS.gene060429 | MS.gene47689 | 0.812449 | 4.22E-51 | -1.69E-46 |
| MS.gene060519 | MS.gene47689 | 0.830341 | 3.10E-55 | -1.69E-46 |
| MS.gene060587 | MS.gene47689 | 0.876175 | 1.72E-68 | -1.69E-46 |
| MS.gene060603 | MS.gene47689 | 0.805923 | 1.06E-49 | -1.69E-46 |
| MS.gene061176 | MS.gene47689 | 0.818386 | 2.01E-52 | -1.69E-46 |
| MS.gene061177 | MS.gene47689 | 0.818038 | 2.41E-52 | -1.69E-46 |
| MS.gene061178 | MS.gene47689 | 0.817814 | 2.71E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47689.t1 | MTR_5g094950 | 48.794 | 539 | 165 | 10 | 1 | 524 | 1 | 443 | 9.11e-125 | 376 |
| MS.gene47689.t1 | MTR_5g095050 | 43.191 | 470 | 182 | 10 | 4 | 456 | 4 | 405 | 3.28e-90 | 287 |
| MS.gene47689.t1 | MTR_5g094970 | 46.032 | 378 | 127 | 6 | 69 | 446 | 5 | 305 | 1.28e-77 | 248 |
| MS.gene47689.t1 | MTR_5g094960 | 45.000 | 260 | 128 | 1 | 257 | 501 | 107 | 366 | 3.06e-52 | 184 |
| MS.gene47689.t1 | MTR_5g094960 | 39.744 | 312 | 91 | 10 | 1 | 299 | 1 | 228 | 1.13e-35 | 139 |
| MS.gene47689.t1 | MTR_5g094920 | 36.622 | 527 | 209 | 17 | 1 | 511 | 1 | 418 | 5.20e-52 | 186 |
| MS.gene47689.t1 | MTR_5g077500 | 45.802 | 262 | 86 | 7 | 210 | 457 | 40 | 259 | 1.93e-42 | 153 |
| MS.gene47689.t1 | MTR_5g094990 | 43.750 | 240 | 117 | 2 | 289 | 510 | 133 | 372 | 1.73e-41 | 155 |
| MS.gene47689.t1 | MTR_5g094990 | 37.759 | 241 | 80 | 7 | 1 | 211 | 1 | 201 | 2.33e-23 | 103 |
| MS.gene47689.t1 | MTR_5g094850 | 42.908 | 282 | 93 | 6 | 3 | 271 | 1 | 227 | 1.21e-38 | 147 |
| MS.gene47689.t1 | MTR_5g094850 | 39.249 | 293 | 123 | 5 | 253 | 507 | 114 | 389 | 2.15e-34 | 135 |
| MS.gene47689.t1 | MTR_5g094850 | 42.908 | 282 | 93 | 6 | 3 | 271 | 1 | 227 | 1.35e-38 | 148 |
| MS.gene47689.t1 | MTR_5g094850 | 38.851 | 296 | 120 | 6 | 253 | 510 | 114 | 386 | 2.74e-33 | 133 |
| MS.gene47689.t1 | MTR_5g094910 | 40.310 | 258 | 109 | 5 | 264 | 521 | 79 | 291 | 2.21e-34 | 134 |
| MS.gene47689.t1 | MTR_5g094910 | 31.868 | 273 | 79 | 8 | 1 | 267 | 1 | 172 | 2.74e-13 | 72.0 |
| MS.gene47689.t1 | MTR_5g095370 | 44.737 | 152 | 59 | 3 | 4 | 154 | 4 | 131 | 8.27e-26 | 105 |
| MS.gene47689.t1 | MTR_5g095390 | 41.872 | 203 | 92 | 6 | 3 | 197 | 1 | 185 | 2.00e-23 | 102 |
| MS.gene47689.t1 | MTR_5g095400 | 46.400 | 125 | 54 | 3 | 31 | 154 | 6 | 118 | 5.17e-18 | 87.4 |
| MS.gene47689.t1 | MTR_1g071620 | 40.881 | 159 | 53 | 4 | 323 | 477 | 71 | 192 | 1.35e-17 | 82.4 |
| MS.gene47689.t1 | MTR_5g095310 | 43.333 | 120 | 55 | 3 | 36 | 154 | 2 | 109 | 4.81e-17 | 83.2 |
| MS.gene47689.t1 | MTR_5g095430 | 45.000 | 120 | 53 | 3 | 36 | 154 | 2 | 109 | 6.62e-17 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 84 sgRNAs with CRISPR-Local
Find 103 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATGACCCAGGTTCGATTT+TGG | 0.197878 | 5.3:-72712320 | None:intergenic |
| GAATTAGAGTTGGAAGTTTC+GGG | 0.232160 | 5.3:+72713291 | MS.gene47689:CDS |
| GGAGAAACTTGCTAGGTTAC+AGG | 0.283073 | 5.3:+72712579 | MS.gene47689:CDS |
| TGAATTAGAGTTGGAAGTTT+CGG | 0.309252 | 5.3:+72713290 | MS.gene47689:CDS |
| GGAAACGTTGAACAACAATT+TGG | 0.315230 | 5.3:+72713443 | MS.gene47689:CDS |
| TGAGTTAGAATCTGAGGTTT+TGG | 0.346512 | 5.3:+72713197 | MS.gene47689:CDS |
| TGAACTAGAGCATCGAGTTT+TGG | 0.347114 | 5.3:+72713029 | MS.gene47689:CDS |
| CAGAAAATGCATATCTCTAT+AGG | 0.354114 | 5.3:-72713850 | None:intergenic |
| GTTTCAAGAGGAGCTTGAAT+TGG | 0.356825 | 5.3:+72712558 | MS.gene47689:CDS |
| CTTCCTTCAACTTCTTGACT+TGG | 0.368972 | 5.3:-72712885 | None:intergenic |
| CGAGCCTCCTGCTGATACTA+TGG | 0.369008 | 5.3:+72713647 | MS.gene47689:CDS |
| TGAGTTAGAGCTTCAAGTTT+TGG | 0.375044 | 5.3:+72712945 | MS.gene47689:CDS |
| TGACAATGTTGAACGTGTTT+TGG | 0.375700 | 5.3:+72712465 | MS.gene47689:CDS |
| AGTAACACTTCAACGAGATC+TGG | 0.378949 | 5.3:-72712418 | None:intergenic |
| TGAGGAAGTTGAAGGATAAG+TGG | 0.396275 | 5.3:+72713223 | MS.gene47689:CDS |
| TTTACAGCACAATGTTGAAA+TGG | 0.412766 | 5.3:+72712525 | MS.gene47689:CDS |
| GGAGAATGATAAAACTGCTT+TGG | 0.414411 | 5.3:+72713389 | MS.gene47689:CDS |
| TTAGCTGATAGAGATACTAT+TGG | 0.415573 | 5.3:+72712829 | MS.gene47689:CDS |
| TCGAAGCAAGGTTCGAGTAT+TGG | 0.418664 | 5.3:+72713368 | MS.gene47689:CDS |
| TTGAAGAAAGTGAAAGTAGC+TGG | 0.420289 | 5.3:+72712676 | MS.gene47689:CDS |
| GAGTCTTTAAACACAAAGTT+CGG | 0.421134 | 5.3:+72713537 | MS.gene47689:CDS |
| AAAGAGGAACATTGAGTTGA+GGG | 0.429427 | 5.3:+72712735 | MS.gene47689:CDS |
| TTTGGAGTTGAGGAAGTTGA+AGG | 0.434593 | 5.3:+72713215 | MS.gene47689:CDS |
| GATTGAAAATGTCAAATTGA+AGG | 0.436713 | 5.3:+72713422 | MS.gene47689:CDS |
| TGAAGAAGTTGAAGGGGAAG+TGG | 0.443788 | 5.3:+72713316 | MS.gene47689:CDS |
| GTCCTCAGGTGCAAAACAAC+GGG | 0.448627 | 5.3:+72713751 | MS.gene47689:CDS |
| AATGATAAAACTGCTTTGGC+TGG | 0.449815 | 5.3:+72713393 | MS.gene47689:CDS |
| TAAAGGTGCACCATCAGGTT+TGG | 0.450161 | 5.3:+72713803 | MS.gene47689:CDS |
| TTCTGTAGAGAAAGATAAAG+AGG | 0.476033 | 5.3:+72713677 | MS.gene47689:CDS |
| TCATCTCTAACAAGAATCAC+AGG | 0.478957 | 5.3:-72712355 | None:intergenic |
| CTGTTGATGTTCTGAGAAAG+AGG | 0.481826 | 5.3:+72712719 | MS.gene47689:CDS |
| GAAGCTAAAGGTGCACCATC+AGG | 0.484062 | 5.3:+72713798 | MS.gene47689:CDS |
| TGTGCTGTAAATGTAGAATC+TGG | 0.488371 | 5.3:-72712513 | None:intergenic |
| CAAGCTAGCTGCCAGTCCTC+AGG | 0.495740 | 5.3:+72713737 | MS.gene47689:CDS |
| AACAAGAATCACAGGGTTGG+TGG | 0.496938 | 5.3:-72712347 | None:intergenic |
| ATCAGCTAAACTAGTTATCT+CGG | 0.497069 | 5.3:-72712815 | None:intergenic |
| GAAGAAGTTGAAGGGGAAGT+GGG | 0.503194 | 5.3:+72713317 | MS.gene47689:CDS |
| TCGGGATTGAAGAAGTTGAA+GGG | 0.511191 | 5.3:+72713309 | MS.gene47689:CDS |
| GTCAAGAAGTTGAAGGAAGA+TGG | 0.513911 | 5.3:+72712889 | MS.gene47689:CDS |
| TTCGGGATTGAAGAAGTTGA+AGG | 0.514395 | 5.3:+72713308 | MS.gene47689:CDS |
| AGACGAGAAGCTCAAGGTTG+AGG | 0.521486 | 5.3:+72713506 | MS.gene47689:CDS |
| GCATGAAAGAGTTGCAAGGT+TGG | 0.521848 | 5.3:+72713566 | MS.gene47689:CDS |
| AACGAATGGTGAATTAGAGT+TGG | 0.523751 | 5.3:+72713281 | MS.gene47689:CDS |
| AAAGTGAAAGTAGCTGGGTT+AGG | 0.525794 | 5.3:+72712682 | MS.gene47689:CDS |
| ATTCAACCCAAAATCGAACC+TGG | 0.527062 | 5.3:+72712313 | MS.gene47689:CDS |
| GAATTATGAGTTAGAATCTG+AGG | 0.528199 | 5.3:+72713191 | MS.gene47689:CDS |
| TGTTGATGTGCTTCGAAGCA+AGG | 0.528265 | 5.3:+72713356 | MS.gene47689:CDS |
| ACTTGCTAGGTTACAGGCTG+AGG | 0.533572 | 5.3:+72712585 | MS.gene47689:CDS |
| TTAATGAGTGTAGATACCTC+TGG | 0.537571 | 5.3:+72713606 | MS.gene47689:CDS |
| ATCTACACTCATTAAAACTT+CGG | 0.544129 | 5.3:-72713598 | None:intergenic |
| TTGAATTGGAGAAACTTGCT+AGG | 0.544441 | 5.3:+72712572 | MS.gene47689:CDS |
| AAGTTGTGAAGCTCGGTTGA+AGG | 0.545324 | 5.3:+72712789 | MS.gene47689:CDS |
| GTAACACTTCAACGAGATCT+GGG | 0.548255 | 5.3:-72712417 | None:intergenic |
| TGAGGAAGGTAAAAGAGAGA+AGG | 0.548951 | 5.3:+72712765 | MS.gene47689:CDS |
| TCAACAACCATAGTATCAGC+AGG | 0.558053 | 5.3:-72713654 | None:intergenic |
| CGGGATTGAAGAAGTTGAAG+GGG | 0.558106 | 5.3:+72713310 | MS.gene47689:CDS |
| TTGTTAGAGTCTGAAGCTAA+AGG | 0.569033 | 5.3:+72713786 | MS.gene47689:CDS |
| AGAAGGAAAGTTGTGAAGCT+CGG | 0.573889 | 5.3:+72712782 | MS.gene47689:CDS |
| TTGAGGGAAATGTGTGAGGA+AGG | 0.576476 | 5.3:+72712751 | MS.gene47689:CDS |
| TGTTAGAGATGAAGAAGCTG+TGG | 0.578329 | 5.3:+72712366 | MS.gene47689:CDS |
| GTGCCAAGTCAAGAAGTTGA+AGG | 0.578672 | 5.3:+72712882 | MS.gene47689:CDS |
| TACATAATTTCCAAACCTGA+TGG | 0.579604 | 5.3:-72713813 | None:intergenic |
| TGAAGAAAGTGAAAGTAGCT+GGG | 0.598840 | 5.3:+72712677 | MS.gene47689:CDS |
| CTCTGGTGGTGGAAACGATG+AGG | 0.601316 | 5.3:+72713623 | MS.gene47689:CDS |
| AGTGTAGATACCTCTGGTGG+TGG | 0.602833 | 5.3:+72713612 | MS.gene47689:CDS |
| GGAGAAACAAAAGTTTCAAG+AGG | 0.606040 | 5.3:+72712546 | MS.gene47689:CDS |
| TGGCAACTATAAGTGAGTTG+AGG | 0.607774 | 5.3:+72713463 | MS.gene47689:CDS |
| TCTGGTGGTGGAAACGATGA+GGG | 0.614819 | 5.3:+72713624 | MS.gene47689:CDS |
| AGTCCTCAGGTGCAAAACAA+CGG | 0.617401 | 5.3:+72713750 | MS.gene47689:CDS |
| GAAAGAGGAACATTGAGTTG+AGG | 0.620134 | 5.3:+72712734 | MS.gene47689:CDS |
| TCTAACAAGAATCACAGGGT+TGG | 0.623457 | 5.3:-72712350 | None:intergenic |
| ATTGGCAGACGAGAAGCTCA+AGG | 0.630706 | 5.3:+72713500 | MS.gene47689:CDS |
| TGAAAGAGTTGCAAGGTTGG+AGG | 0.631396 | 5.3:+72713569 | MS.gene47689:CDS |
| AGATGACAACACACTTAATG+TGG | 0.632788 | 5.3:+72713158 | MS.gene47689:CDS |
| TTCAACCCAAAATCGAACCT+GGG | 0.633225 | 5.3:+72712314 | MS.gene47689:CDS |
| AATTGCATGAAAGAGTTGCA+AGG | 0.633271 | 5.3:+72713562 | MS.gene47689:CDS |
| CATCTCTAACAAGAATCACA+GGG | 0.641894 | 5.3:-72712354 | None:intergenic |
| CTCATCGTTTCCACCACCAG+AGG | 0.650565 | 5.3:-72713622 | None:intergenic |
| GTTGCGCTAAACAAAACGAA+TGG | 0.654742 | 5.3:+72713267 | MS.gene47689:CDS |
| TTGGTGGAAGATGATGACCC+AGG | 0.663433 | 5.3:-72712331 | None:intergenic |
| GCAAAACAACGGGAGCAAAG+AGG | 0.667911 | 5.3:+72713761 | MS.gene47689:CDS |
| TGAGTTGAGGGAAATGTGTG+AGG | 0.685982 | 5.3:+72712747 | MS.gene47689:CDS |
| ACAACCATAGTATCAGCAGG+AGG | 0.723572 | 5.3:-72713651 | None:intergenic |
| ATGAGTGTAGATACCTCTGG+TGG | 0.724549 | 5.3:+72713609 | MS.gene47689:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACAAAGAAAAACAATGAGT+TGG | + | chr5.3:72712858-72712877 | MS.gene47689:CDS | 25.0% |
| ! | ATCTACACTCATTAAAACTT+CGG | - | chr5.3:72713601-72713620 | None:intergenic | 25.0% |
| ! | GATTGAAAATGTCAAATTGA+AGG | + | chr5.3:72713422-72713441 | MS.gene47689:CDS | 25.0% |
| ! | TAAGAAAGTGAAAAAACTAG+AGG | + | chr5.3:72712636-72712655 | MS.gene47689:CDS | 25.0% |
| !! | TTTAGAGTTGAAGGAAAAAA+GGG | + | chr5.3:72713131-72713150 | MS.gene47689:CDS | 25.0% |
| !! | TTTGGAGTTGAACAAAAAAA+AGG | + | chr5.3:72713047-72713066 | MS.gene47689:CDS | 25.0% |
| !!! | TTTTAGAGTTGAAGGAAAAA+AGG | + | chr5.3:72713130-72713149 | MS.gene47689:CDS | 25.0% |
| AAGTGAAAAAACTAGAGGAA+CGG | + | chr5.3:72712641-72712660 | MS.gene47689:CDS | 30.0% | |
| ATCAGCTAAACTAGTTATCT+CGG | - | chr5.3:72712818-72712837 | None:intergenic | 30.0% | |
| CAGAAAATGCATATCTCTAT+AGG | - | chr5.3:72713853-72713872 | None:intergenic | 30.0% | |
| GAATTATGAGTTAGAATCTG+AGG | + | chr5.3:72713191-72713210 | MS.gene47689:CDS | 30.0% | |
| GAGGAGCAAAAAGATAAAAT+TGG | + | chr5.3:72713482-72713501 | MS.gene47689:CDS | 30.0% | |
| GAGTCTTTAAACACAAAGTT+CGG | + | chr5.3:72713537-72713556 | MS.gene47689:CDS | 30.0% | |
| TACATAATTTCCAAACCTGA+TGG | - | chr5.3:72713816-72713835 | None:intergenic | 30.0% | |
| TGAATTAGAGTTGGAAGTTT+CGG | + | chr5.3:72713290-72713309 | MS.gene47689:CDS | 30.0% | |
| TTAGCTGATAGAGATACTAT+TGG | + | chr5.3:72712829-72712848 | MS.gene47689:CDS | 30.0% | |
| TTCTGTAGAGAAAGATAAAG+AGG | + | chr5.3:72713677-72713696 | MS.gene47689:CDS | 30.0% | |
| TTTACAGCACAATGTTGAAA+TGG | + | chr5.3:72712525-72712544 | MS.gene47689:CDS | 30.0% | |
| ! | ACAATTTTGTTGATGTGCTA+AGG | + | chr5.3:72712995-72713014 | MS.gene47689:CDS | 30.0% |
| !! | TTTGGTGTTGAACGAAAAAA+GGG | + | chr5.3:72712963-72712982 | MS.gene47689:CDS | 30.0% |
| !!! | TTTTGGTGTTGAACGAAAAA+AGG | + | chr5.3:72712962-72712981 | MS.gene47689:CDS | 30.0% |
| AACGAATGGTGAATTAGAGT+TGG | + | chr5.3:72713281-72713300 | MS.gene47689:CDS | 35.0% | |
| AATGATAAAACTGCTTTGGC+TGG | + | chr5.3:72713393-72713412 | MS.gene47689:CDS | 35.0% | |
| AATTGCATGAAAGAGTTGCA+AGG | + | chr5.3:72713562-72713581 | MS.gene47689:CDS | 35.0% | |
| AGATGACAACACACTTAATG+TGG | + | chr5.3:72713158-72713177 | MS.gene47689:CDS | 35.0% | |
| CATCTCTAACAAGAATCACA+GGG | - | chr5.3:72712357-72712376 | None:intergenic | 35.0% | |
| GAATTAGAGTTGGAAGTTTC+GGG | + | chr5.3:72713291-72713310 | MS.gene47689:CDS | 35.0% | |
| GGAAACGTTGAACAACAATT+TGG | + | chr5.3:72713443-72713462 | MS.gene47689:CDS | 35.0% | |
| GGAGAAACAAAAGTTTCAAG+AGG | + | chr5.3:72712546-72712565 | MS.gene47689:CDS | 35.0% | |
| GGAGAATGATAAAACTGCTT+TGG | + | chr5.3:72713389-72713408 | MS.gene47689:CDS | 35.0% | |
| GGAGTTGAACAAAAAAAAGG+TGG | + | chr5.3:72713050-72713069 | MS.gene47689:CDS | 35.0% | |
| TCATCTCTAACAAGAATCAC+AGG | - | chr5.3:72712358-72712377 | None:intergenic | 35.0% | |
| TGAAGAAAGTGAAAGTAGCT+GGG | + | chr5.3:72712677-72712696 | MS.gene47689:CDS | 35.0% | |
| TGTGCTGTAAATGTAGAATC+TGG | - | chr5.3:72712516-72712535 | None:intergenic | 35.0% | |
| TTAATGAGTGTAGATACCTC+TGG | + | chr5.3:72713606-72713625 | MS.gene47689:CDS | 35.0% | |
| TTGAAGAAAGTGAAAGTAGC+TGG | + | chr5.3:72712676-72712695 | MS.gene47689:CDS | 35.0% | |
| ! | TGACAATGTTGAACGTGTTT+TGG | + | chr5.3:72712465-72712484 | MS.gene47689:CDS | 35.0% |
| ! | TTGAATTGGAGAAACTTGCT+AGG | + | chr5.3:72712572-72712591 | MS.gene47689:CDS | 35.0% |
| ! | TTGTTAGAGTCTGAAGCTAA+AGG | + | chr5.3:72713786-72713805 | MS.gene47689:CDS | 35.0% |
| !! | AAAGAGGAACATTGAGTTGA+GGG | + | chr5.3:72712735-72712754 | MS.gene47689:CDS | 35.0% |
| !! | TGAGTTAGAATCTGAGGTTT+TGG | + | chr5.3:72713197-72713216 | MS.gene47689:CDS | 35.0% |
| !! | TGAGTTAGAGCTTCAAGTTT+TGG | + | chr5.3:72712945-72712964 | MS.gene47689:CDS | 35.0% |
| !!! | GCATGAAGTTTTAGAGTTGA+AGG | + | chr5.3:72713122-72713141 | MS.gene47689:CDS | 35.0% |
| AAAGTGAAAGTAGCTGGGTT+AGG | + | chr5.3:72712682-72712701 | MS.gene47689:CDS | 40.0% | |
| AGAAGGAAAGTTGTGAAGCT+CGG | + | chr5.3:72712782-72712801 | MS.gene47689:CDS | 40.0% | |
| AGAGTTGAAGGAAAAAAGGG+TGG | + | chr5.3:72713134-72713153 | MS.gene47689:CDS | 40.0% | |
| AGTAACACTTCAACGAGATC+TGG | - | chr5.3:72712421-72712440 | None:intergenic | 40.0% | |
| ATTCAACCCAAAATCGAACC+TGG | + | chr5.3:72712313-72712332 | MS.gene47689:CDS | 40.0% | |
| CTTCCTTCAACTTCTTGACT+TGG | - | chr5.3:72712888-72712907 | None:intergenic | 40.0% | |
| GAGTTGAAGGAAAAAAGGGT+GGG | + | chr5.3:72713135-72713154 | MS.gene47689:CDS | 40.0% | |
| GTAACACTTCAACGAGATCT+GGG | - | chr5.3:72712420-72712439 | None:intergenic | 40.0% | |
| GTCAAGAAGTTGAAGGAAGA+TGG | + | chr5.3:72712889-72712908 | MS.gene47689:CDS | 40.0% | |
| GTTGCGCTAAACAAAACGAA+TGG | + | chr5.3:72713267-72713286 | MS.gene47689:CDS | 40.0% | |
| GTTTCAAGAGGAGCTTGAAT+TGG | + | chr5.3:72712558-72712577 | MS.gene47689:CDS | 40.0% | |
| TCAACAACCATAGTATCAGC+AGG | - | chr5.3:72713657-72713676 | None:intergenic | 40.0% | |
| TCGGGATTGAAGAAGTTGAA+GGG | + | chr5.3:72713309-72713328 | MS.gene47689:CDS | 40.0% | |
| TCTAACAAGAATCACAGGGT+TGG | - | chr5.3:72712353-72712372 | None:intergenic | 40.0% | |
| TGAGGAAGGTAAAAGAGAGA+AGG | + | chr5.3:72712765-72712784 | MS.gene47689:CDS | 40.0% | |
| TGAGGAAGTTGAAGGATAAG+TGG | + | chr5.3:72713223-72713242 | MS.gene47689:CDS | 40.0% | |
| TGGCAACTATAAGTGAGTTG+AGG | + | chr5.3:72713463-72713482 | MS.gene47689:CDS | 40.0% | |
| TGTTAGAGATGAAGAAGCTG+TGG | + | chr5.3:72712366-72712385 | MS.gene47689:CDS | 40.0% | |
| TTCAACCCAAAATCGAACCT+GGG | + | chr5.3:72712314-72712333 | MS.gene47689:CDS | 40.0% | |
| TTCGGGATTGAAGAAGTTGA+AGG | + | chr5.3:72713308-72713327 | MS.gene47689:CDS | 40.0% | |
| ! | GAAAGAGGAACATTGAGTTG+AGG | + | chr5.3:72712734-72712753 | MS.gene47689:CDS | 40.0% |
| ! | TTTGGAGTTGAGGAAGTTGA+AGG | + | chr5.3:72713215-72713234 | MS.gene47689:CDS | 40.0% |
| !! | CTGTTGATGTTCTGAGAAAG+AGG | + | chr5.3:72712719-72712738 | MS.gene47689:CDS | 40.0% |
| !!! | AATCTGAGGTTTTGGAGTTG+AGG | + | chr5.3:72713205-72713224 | MS.gene47689:CDS | 40.0% |
| !!! | TGAACTAGAGCATCGAGTTT+TGG | + | chr5.3:72713029-72713048 | MS.gene47689:CDS | 40.0% |
| AACAAGAATCACAGGGTTGG+TGG | - | chr5.3:72712350-72712369 | None:intergenic | 45.0% | |
| AAGTTGTGAAGCTCGGTTGA+AGG | + | chr5.3:72712789-72712808 | MS.gene47689:CDS | 45.0% | |
| ACAACCATAGTATCAGCAGG+AGG | - | chr5.3:72713654-72713673 | None:intergenic | 45.0% | |
| AGTCCTCAGGTGCAAAACAA+CGG | + | chr5.3:72713750-72713769 | MS.gene47689:CDS | 45.0% | |
| ATGAGTGTAGATACCTCTGG+TGG | + | chr5.3:72713609-72713628 | MS.gene47689:CDS | 45.0% | |
| CGGGATTGAAGAAGTTGAAG+GGG | + | chr5.3:72713310-72713329 | MS.gene47689:CDS | 45.0% | |
| GAAGAAGTTGAAGGGGAAGT+GGG | + | chr5.3:72713317-72713336 | MS.gene47689:CDS | 45.0% | |
| GCATGAAAGAGTTGCAAGGT+TGG | + | chr5.3:72713566-72713585 | MS.gene47689:CDS | 45.0% | |
| GTGCCAAGTCAAGAAGTTGA+AGG | + | chr5.3:72712882-72712901 | MS.gene47689:CDS | 45.0% | |
| TCGAAGCAAGGTTCGAGTAT+TGG | + | chr5.3:72713368-72713387 | MS.gene47689:CDS | 45.0% | |
| TGAAAGAGTTGCAAGGTTGG+AGG | + | chr5.3:72713569-72713588 | MS.gene47689:CDS | 45.0% | |
| TGAAGAAGTTGAAGGGGAAG+TGG | + | chr5.3:72713316-72713335 | MS.gene47689:CDS | 45.0% | |
| TGTTGATGTGCTTCGAAGCA+AGG | + | chr5.3:72713356-72713375 | MS.gene47689:CDS | 45.0% | |
| TTGAGGGAAATGTGTGAGGA+AGG | + | chr5.3:72712751-72712770 | MS.gene47689:CDS | 45.0% | |
| ! | GATGACCCAGGTTCGATTTT+GGG | - | chr5.3:72712322-72712341 | None:intergenic | 45.0% |
| ! | GGTGTTGAACGAAAAAAGGG+TGG | + | chr5.3:72712966-72712985 | MS.gene47689:CDS | 45.0% |
| ! | TAAAGGTGCACCATCAGGTT+TGG | + | chr5.3:72713803-72713822 | MS.gene47689:CDS | 45.0% |
| ! | TGAGTTGAGGGAAATGTGTG+AGG | + | chr5.3:72712747-72712766 | MS.gene47689:CDS | 45.0% |
| ! | TGATGACCCAGGTTCGATTT+TGG | - | chr5.3:72712323-72712342 | None:intergenic | 45.0% |
| !! | GGAGAAACTTGCTAGGTTAC+AGG | + | chr5.3:72712579-72712598 | MS.gene47689:CDS | 45.0% |
| AGACGAGAAGCTCAAGGTTG+AGG | + | chr5.3:72713506-72713525 | MS.gene47689:CDS | 50.0% | |
| AGTGTAGATACCTCTGGTGG+TGG | + | chr5.3:72713612-72713631 | MS.gene47689:CDS | 50.0% | |
| ATTGGCAGACGAGAAGCTCA+AGG | + | chr5.3:72713500-72713519 | MS.gene47689:CDS | 50.0% | |
| GCAAAACAACGGGAGCAAAG+AGG | + | chr5.3:72713761-72713780 | MS.gene47689:CDS | 50.0% | |
| GTCCTCAGGTGCAAAACAAC+GGG | + | chr5.3:72713751-72713770 | MS.gene47689:CDS | 50.0% | |
| TTGGTGGAAGATGATGACCC+AGG | - | chr5.3:72712334-72712353 | None:intergenic | 50.0% | |
| ! | ACTTGCTAGGTTACAGGCTG+AGG | + | chr5.3:72712585-72712604 | MS.gene47689:CDS | 50.0% |
| ! | GAAGCTAAAGGTGCACCATC+AGG | + | chr5.3:72713798-72713817 | MS.gene47689:CDS | 50.0% |
| !! | GTTGTTTTGCACCTGAGGAC+TGG | - | chr5.3:72713751-72713770 | None:intergenic | 50.0% |
| !! | TCTGGTGGTGGAAACGATGA+GGG | + | chr5.3:72713624-72713643 | MS.gene47689:CDS | 50.0% |
| CGAGCCTCCTGCTGATACTA+TGG | + | chr5.3:72713647-72713666 | MS.gene47689:CDS | 55.0% | |
| CTCATCGTTTCCACCACCAG+AGG | - | chr5.3:72713625-72713644 | None:intergenic | 55.0% | |
| !! | CTCCCGTTGTTTTGCACCTG+AGG | - | chr5.3:72713756-72713775 | None:intergenic | 55.0% |
| !! | CTCTGGTGGTGGAAACGATG+AGG | + | chr5.3:72713623-72713642 | MS.gene47689:CDS | 55.0% |
| CAAGCTAGCTGCCAGTCCTC+AGG | + | chr5.3:72713737-72713756 | MS.gene47689:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 72712307 | 72713887 | 72712307 | ID=MS.gene47689 |
| chr5.3 | mRNA | 72712307 | 72713887 | 72712307 | ID=MS.gene47689.t1;Parent=MS.gene47689 |
| chr5.3 | exon | 72712307 | 72713887 | 72712307 | ID=MS.gene47689.t1.exon1;Parent=MS.gene47689.t1 |
| chr5.3 | CDS | 72712307 | 72713887 | 72712307 | ID=cds.MS.gene47689.t1;Parent=MS.gene47689.t1 |
| Gene Sequence |
| Protein sequence |
