AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30882


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30882.t1 MTR_1g067220 95.102 245 7 3 35 278 1 241 6.92e-161 447
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30882.t1 AT2G37300 66.667 48 16 0 45 92 13 60 7.50e-14 68.6
MS.gene30882.t1 AT2G37300 66.667 48 16 0 45 92 13 60 7.50e-14 68.6
MS.gene30882.t1 AT2G37300 66.667 48 16 0 45 92 35 82 8.19e-14 68.9
MS.gene30882.t1 AT2G37300 66.667 48 16 0 45 92 35 82 8.19e-14 68.9
MS.gene30882.t1 AT2G37300 66.667 48 16 0 45 92 35 82 8.19e-14 68.9
MS.gene30882.t1 AT2G37300 66.667 48 16 0 45 92 92 139 3.11e-13 68.2

Find 32 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGCTTCTGAATAATATT+TGG 0.158875 1.2:+50576400 None:intergenic
TTCAAAGTTCCTTAGGAATA+AGG 0.235437 1.2:-50575905 MS.gene30882:CDS
TAAGGAACAAGCAGAATTAA+TGG 0.292596 1.2:-50576805 MS.gene30882:CDS
AGATTCCTTTGATAATTCTT+TGG 0.310548 1.2:-50576048 MS.gene30882:CDS
AGCAGAATTAATGGCCAATA+AGG 0.318363 1.2:-50576796 MS.gene30882:intron
ATCCTCCAAATCAAAACAAT+TGG 0.343305 1.2:-50576495 MS.gene30882:CDS
TCCTCCAAATCAAAACAATT+GGG 0.405024 1.2:-50576494 MS.gene30882:CDS
GAAAAGCTTCAAAGTTCCTT+AGG 0.408448 1.2:-50575912 MS.gene30882:CDS
CCAAACAGGACCACTATTGC+AGG 0.439932 1.2:-50577026 MS.gene30882:intron
TGAAGCTCTAGATGCATTGA+GGG 0.457114 1.2:-50575943 MS.gene30882:CDS
CCTGCAATAGTGGTCCTGTT+TGG 0.464615 1.2:+50577026 None:intergenic
ACAGGCTTCAAAAGTAGCTA+AGG 0.474167 1.2:-50576823 MS.gene30882:CDS
AAATTCTCAAACTCAGAGCT+TGG 0.475080 1.2:+50576266 None:intergenic
ATTTGAAGAATGATGAAAGC+AGG 0.488578 1.2:-50576247 MS.gene30882:intron
GAGGTTACTAGCAGAGAGAC+AGG 0.488693 1.2:-50576841 MS.gene30882:CDS
TCGGAGTGAGAAGTTACATT+AGG 0.490921 1.2:+50575865 None:intergenic
AACACTGTGGCATGTCTAAG+AGG 0.493592 1.2:-50576864 MS.gene30882:CDS
CAGAATTGTGAAAATTCTTG+TGG 0.501754 1.2:-50576455 MS.gene30882:CDS
CATAACTGCTAACACCAAAC+AGG 0.505299 1.2:-50577040 MS.gene30882:CDS
AATCGTTCGCCTTATTCCTA+AGG 0.511083 1.2:+50575896 None:intergenic
TTGAAGCTCTAGATGCATTG+AGG 0.524675 1.2:-50575944 MS.gene30882:CDS
GGGAGAGATACCTGCAATAG+TGG 0.525589 1.2:+50577016 None:intergenic
AAGAAAATGGAAGTAGATGA+AGG 0.533758 1.2:-50576897 MS.gene30882:CDS
AGAATTGTGAAAATTCTTGT+GGG 0.556054 1.2:-50576454 MS.gene30882:CDS
GCTGTCACATTCACAGTCAC+AGG 0.564805 1.2:+50576017 None:intergenic
AGATGCATTGAGGGTTGCCA+AGG 0.577687 1.2:-50575934 MS.gene30882:CDS
CCTCCAAATCAAAACAATTG+GGG 0.608612 1.2:-50576493 MS.gene30882:CDS
TAATGCCAAAGAATTATCAA+AGG 0.628211 1.2:+50576043 None:intergenic
ATAAGGCGAACGATTCGCGT+TGG 0.628535 1.2:-50575888 MS.gene30882:CDS
ACACTGTGGCATGTCTAAGA+GGG 0.632958 1.2:-50576863 MS.gene30882:CDS
CTGTGGCATGTCTAAGAGGG+AGG 0.653297 1.2:-50576860 MS.gene30882:CDS
AGGTTTGAATTTGAACACTG+TGG 0.756754 1.2:-50576877 MS.gene30882:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATATTATTACCTTAT+TGG + chr1.2:50576198-50576217 None:intergenic 10.0%
!! ATTTGCTTCTGAATAATATT+TGG + chr1.2:50576580-50576599 None:intergenic 20.0%
!!! AGAGAAAGCTTAAATTTTTA+AGG - chr1.2:50576436-50576455 MS.gene30882:CDS 20.0%
! AGAATTGTGAAAATTCTTGT+GGG - chr1.2:50576523-50576542 MS.gene30882:CDS 25.0%
! CATTGTTACTATCAGTTAAA+AGG + chr1.2:50576222-50576241 None:intergenic 25.0%
! GAATGACATTGTTGAATTAT+TGG - chr1.2:50576047-50576066 MS.gene30882:CDS 25.0%
! TAATGCCAAAGAATTATCAA+AGG + chr1.2:50576937-50576956 None:intergenic 25.0%
!! AGATTCCTTTGATAATTCTT+TGG - chr1.2:50576929-50576948 MS.gene30882:intron 25.0%
!! TGTTTAGAACATCATCATAA+AGG - chr1.2:50576266-50576285 MS.gene30882:CDS 25.0%
!!! GAAAGTTATTTTTTGACAGT+TGG - chr1.2:50576362-50576381 MS.gene30882:CDS 25.0%
!!! TGTTGTCAATTTTGGAATTT+TGG - chr1.2:50576837-50576856 MS.gene30882:CDS 25.0%
AAGAAAATGGAAGTAGATGA+AGG - chr1.2:50576080-50576099 MS.gene30882:CDS 30.0%
ATCCTCCAAATCAAAACAAT+TGG - chr1.2:50576482-50576501 MS.gene30882:CDS 30.0%
ATTTGAAGAATGATGAAAGC+AGG - chr1.2:50576730-50576749 MS.gene30882:intron 30.0%
CAGAATTGTGAAAATTCTTG+TGG - chr1.2:50576522-50576541 MS.gene30882:CDS 30.0%
TAAGGAACAAGCAGAATTAA+TGG - chr1.2:50576172-50576191 MS.gene30882:intron 30.0%
TCCTCCAAATCAAAACAATT+GGG - chr1.2:50576483-50576502 MS.gene30882:CDS 30.0%
TTCAAAGTTCCTTAGGAATA+AGG - chr1.2:50577072-50577091 MS.gene30882:CDS 30.0%
! AGAATCACAATCTTTTGATG+TGG + chr1.2:50576658-50576677 None:intergenic 30.0%
! TTTGAGCATGTTGTCAATTT+TGG - chr1.2:50576829-50576848 MS.gene30882:CDS 30.0%
!!! ATTTTGGTGTCATGTTTTTC+AGG - chr1.2:50576853-50576872 MS.gene30882:CDS 30.0%
!!! GTCTCTGAAACATTTTGATT+TGG + chr1.2:50576621-50576640 None:intergenic 30.0%
!!! TGGTTTTAACAGCAAGAAAA+TGG - chr1.2:50576067-50576086 MS.gene30882:CDS 30.0%
AAATTCTCAAACTCAGAGCT+TGG + chr1.2:50576714-50576733 None:intergenic 35.0%
AGCAGAATTAATGGCCAATA+AGG - chr1.2:50576181-50576200 MS.gene30882:intron 35.0%
AGGTTTGAATTTGAACACTG+TGG - chr1.2:50576100-50576119 MS.gene30882:CDS 35.0%
CCTCCAAATCAAAACAATTG+GGG - chr1.2:50576484-50576503 MS.gene30882:CDS 35.0%
GAAAAGCTTCAAAGTTCCTT+AGG - chr1.2:50577065-50577084 MS.gene30882:CDS 35.0%
! TTTTTGACAGTTGGTTGAGT+TGG - chr1.2:50576371-50576390 MS.gene30882:CDS 35.0%
!! GTTGGTTGAGTTGGAAAAAT+TGG - chr1.2:50576380-50576399 MS.gene30882:CDS 35.0%
!!! ATGCCCCAATTGTTTTGATT+TGG + chr1.2:50576490-50576509 None:intergenic 35.0%
ACAGGCTTCAAAAGTAGCTA+AGG - chr1.2:50576154-50576173 MS.gene30882:intron 40.0%
CATAACTGCTAACACCAAAC+AGG - chr1.2:50575937-50575956 MS.gene30882:CDS 40.0%
! AATCGTTCGCCTTATTCCTA+AGG + chr1.2:50577084-50577103 None:intergenic 40.0%
!! TGAAGCTCTAGATGCATTGA+GGG - chr1.2:50577034-50577053 MS.gene30882:CDS 40.0%
!! TTGAAGCTCTAGATGCATTG+AGG - chr1.2:50577033-50577052 MS.gene30882:CDS 40.0%
!!! CCCCAATTGTTTTGATTTGG+AGG + chr1.2:50576487-50576506 None:intergenic 40.0%
!!! GGAACTTTGAAGCTTTTCCT+TGG + chr1.2:50577063-50577082 None:intergenic 40.0%
AACACTGTGGCATGTCTAAG+AGG - chr1.2:50576113-50576132 MS.gene30882:intron 45.0%
ACACTGTGGCATGTCTAAGA+GGG - chr1.2:50576114-50576133 MS.gene30882:intron 45.0%
AGAGAGAGAGAGAGAGAGAG+AGG + chr1.2:50575983-50576002 None:intergenic 50.0%
AGATGCATTGAGGGTTGCCA+AGG - chr1.2:50577043-50577062 MS.gene30882:CDS 50.0%
ATAAGGCGAACGATTCGCGT+TGG - chr1.2:50577089-50577108 MS.gene30882:CDS 50.0%
CCAAACAGGACCACTATTGC+AGG - chr1.2:50575951-50575970 MS.gene30882:CDS 50.0%
CCTGCAATAGTGGTCCTGTT+TGG + chr1.2:50575954-50575973 None:intergenic 50.0%
GAGAGAGAGAGAGAGAGAGA+GGG + chr1.2:50575984-50576003 None:intergenic 50.0%
GAGGTTACTAGCAGAGAGAC+AGG - chr1.2:50576136-50576155 MS.gene30882:intron 50.0%
GCTGTCACATTCACAGTCAC+AGG + chr1.2:50576963-50576982 None:intergenic 50.0%
GGGAGAGATACCTGCAATAG+TGG + chr1.2:50575964-50575983 None:intergenic 50.0%
CTGTGGCATGTCTAAGAGGG+AGG - chr1.2:50576117-50576136 MS.gene30882:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 50575878 50577121 50575878 ID=MS.gene30882
chr1.2 mRNA 50575878 50577121 50575878 ID=MS.gene30882.t1;Parent=MS.gene30882
chr1.2 exon 50577027 50577121 50577027 ID=MS.gene30882.t1.exon1;Parent=MS.gene30882.t1
chr1.2 CDS 50577027 50577121 50577027 ID=cds.MS.gene30882.t1;Parent=MS.gene30882.t1
chr1.2 exon 50576797 50576920 50576797 ID=MS.gene30882.t1.exon2;Parent=MS.gene30882.t1
chr1.2 CDS 50576797 50576920 50576797 ID=cds.MS.gene30882.t1;Parent=MS.gene30882.t1
chr1.2 exon 50576248 50576618 50576248 ID=MS.gene30882.t1.exon3;Parent=MS.gene30882.t1
chr1.2 CDS 50576248 50576618 50576248 ID=cds.MS.gene30882.t1;Parent=MS.gene30882.t1
chr1.2 exon 50575878 50576124 50575878 ID=MS.gene30882.t1.exon4;Parent=MS.gene30882.t1
chr1.2 CDS 50575878 50576124 50575878 ID=cds.MS.gene30882.t1;Parent=MS.gene30882.t1
Gene Sequence

>MS.gene30882

ATGGGAACTTTCTCTGCTCAAACTGAAACTGAAACTTCAGATCATAAGCAAATGCATGTCATAACTGCTAACACCAAACAGGACCACTATTGCAGGTATCTCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAATATATATATTAAATTGTGAAAAATTGAATGACATTGTTGAATTATTGGTTTTAACAGCAAGAAAATGGAAGTAGATGAAGGTTTGAATTTGAACACTGTGGCATGTCTAAGAGGGAGGTTACTAGCAGAGAGACAGGCTTCAAAAGTAGCTAAGGAACAAGCAGAATTAATGGCCAATAAGGTAATAATATTTTTTACCTTTTAACTGATAGTAACAATGTTTCAATTCACTTTTCTGTGACATTGTTTAGAACATCATCATAAAGGAAAGATGAAAATGACACTGATGTTTTTCTGACATAACTTTTATTTTGTTTGTGTTTCTATTTCTGAAATAATTGAAAGTTATTTTTTGACAGTTGGTTGAGTTGGAAAAATTGGTTAAAGAAGAGATCAAATTAAGAGACAAATCTGAGAGAAAGCTTAAATTTTTAAGGAAAAAGCTTGAATCTTTTAGCAAATCCTCCAAATCAAAACAATTGGGGCATTCTGATTCATCACAGAATTGTGAAAATTCTTGTGGGTCATCATCATCAATTAGTTCAAATTCAAATTCCAAATATTATTCAGAAGCAAATGAAACACAAAATCTAGTACCAAATCAAAATGTTTCAGAGACACAGAATAGTTCATCCACATCAAAAGATTGTGATTCTCAGATTACTGATGATTCAAGTAGTAATTCAAAACCAAGCTCTGAGTTTGAGAATTTGAAGAATGATGAAAGCAGGTATTATTAGATTTGAACATGTGATTATTTTTGTTTGTTTGTATTTTTTATGAATTGTTGTTTTTTAATTATATGTTTTTGAGCATGTTGTCAATTTTGGAATTTTGGTGTCATGTTTTTCAGGATTTCTAGTTTAAGCACAAAATCATCAGTGACAGAGAATGAAAATGATCATGCAGATTCCTTTGATAATTCTTTGGCATTAGTTCCTGTGACTGTGAATGTGACAGCAACATCTCAAGCTACTGCAACTAATCACAAACAAGTCAATGAAAGTGTCTTTGAAGCTCTAGATGCATTGAGGGTTGCCAAGGAAAAGCTTCAAAGTTCCTTAGGAATAAGGCGAACGATTCGCGTTGGCCTAATGTAA

Protein sequence

>MS.gene30882.t1

MGTFSAQTETETSDHKQMHVITANTKQDHYCSKKMEVDEGLNLNTVACLRGRLLAERQASKVAKEQAELMANKLVELEKLVKEEIKLRDKSERKLKFLRKKLESFSKSSKSKQLGHSDSSQNCENSCGSSSSISSNSNSKYYSEANETQNLVPNQNVSETQNSSSTSKDCDSQITDDSSSNSKPSSEFENLKNDESRISSLSTKSSVTENENDHADSFDNSLALVPVTVNVTATSQATATNHKQVNESVFEALDALRVAKEKLQSSLGIRRTIRVGLM