AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79960


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79960.t1 MTR_7g026320 100.000 227 0 0 1 227 1 227 1.58e-171 471
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79960.t1 AT3G55420 65.534 206 60 3 26 227 18 216 4.24e-85 252

Find 35 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGAGCTTAAAGATTTCT+TGG 0.245364 7.2:+72992016 MS.gene79960:CDS
GTGTATGCTGTTAGAGAAAT+TGG 0.256026 7.2:+72991942 MS.gene79960:CDS
AAACCAACCTCAGAAGTTAA+AGG 0.274880 7.2:+72991534 MS.gene79960:CDS
AAATGGCTAGGTTCAAATTC+AGG 0.286811 7.2:+72991898 MS.gene79960:CDS
TAATTAATACTTCTTTGATT+TGG 0.320121 7.2:-72992062 None:intergenic
GTTAGAGAAATTGGAAGCTT+TGG 0.336072 7.2:+72991951 MS.gene79960:CDS
GAAGTTAAAGGTATAAGATT+TGG 0.355820 7.2:+72991546 MS.gene79960:CDS
AGTGTTCTGTTGATAGAATT+TGG 0.356899 7.2:+72991814 MS.gene79960:CDS
TTACTCTCAATCTCAAAATC+AGG 0.362211 7.2:+72992082 MS.gene79960:CDS
AACTTCTGAGGTTGGTTTGA+AGG 0.367774 7.2:-72991529 None:intergenic
TGTGAACAAGAAACTCATAA+AGG 0.455332 7.2:+72991860 MS.gene79960:CDS
GCTAGGATTGTGAAATTTGT+TGG 0.455562 7.2:-72991705 None:intergenic
GTGAACAAGAAACTCATAAA+GGG 0.463123 7.2:+72991861 MS.gene79960:CDS
AAACTCATAAAGGGTCTTTG+TGG 0.468332 7.2:+72991870 MS.gene79960:CDS
ATACCTTTAACTTCTGAGGT+TGG 0.471100 7.2:-72991537 None:intergenic
ATCTCAAAATCAGGTTGCTA+TGG 0.477887 7.2:+72992091 MS.gene79960:CDS
ATTTCTTTGATCCAAGCCCT+AGG 0.480022 7.2:-72991752 None:intergenic
GGCAAGAATGCTGTTGTTGA+AGG 0.519600 7.2:-72991684 None:intergenic
TCTTATACCTTTAACTTCTG+AGG 0.522452 7.2:-72991541 None:intergenic
TGTGTTTGTGTTTGAAACTG+AGG 0.526748 7.2:+72991785 MS.gene79960:CDS
GTTAAAGGTATAAGATTTGG+TGG 0.527957 7.2:+72991549 MS.gene79960:CDS
TTGTTTGAGAATTAGGATGT+TGG 0.533338 7.2:-72991485 None:intergenic
AGAACTTTGAGAAGTTCAAG+AGG 0.536254 7.2:-72991612 None:intergenic
CACACACTAACCCTAGGGCT+TGG 0.546093 7.2:+72991741 MS.gene79960:CDS
TGTGCCATGCTTGATGTGCT+AGG 0.551608 7.2:-72991722 None:intergenic
GCATGGCACACACTAACCCT+AGG 0.552987 7.2:+72991735 MS.gene79960:CDS
GCTAGGTTCAAATTCAGGAA+AGG 0.582435 7.2:+72991903 MS.gene79960:CDS
TTTCTTTGATCCAAGCCCTA+GGG 0.582873 7.2:-72991751 None:intergenic
TGGCCACATGAAATAGCACT+TGG 0.594109 7.2:+72991834 MS.gene79960:CDS
AATGCTGTTGTTGAAGGGAA+AGG 0.602603 7.2:-72991678 None:intergenic
TGGTTTGAAGGAGAAATCTG+AGG 0.603282 7.2:-72991517 None:intergenic
CAATCCTAGCACATCAAGCA+TGG 0.615793 7.2:+72991718 MS.gene79960:CDS
GCAAGAATGCTGTTGTTGAA+GGG 0.631568 7.2:-72991683 None:intergenic
CATGGCACACACTAACCCTA+GGG 0.642128 7.2:+72991736 MS.gene79960:CDS
TCACCAAGTGCTATTTCATG+TGG 0.645451 7.2:-72991837 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAATACTTCTTTGATT+TGG - chr7.2:72992065-72992084 None:intergenic 15.0%
!!! GAAATGATTTTACTATGAAT+TGG - chr7.2:72991575-72991594 None:intergenic 20.0%
!! GAAGTTAAAGGTATAAGATT+TGG + chr7.2:72991546-72991565 MS.gene79960:CDS 25.0%
!!! TGTTTTGTTGTTTGAGAATT+AGG - chr7.2:72991495-72991514 None:intergenic 25.0%
GTGAACAAGAAACTCATAAA+GGG + chr7.2:72991861-72991880 MS.gene79960:CDS 30.0%
TGTGAACAAGAAACTCATAA+AGG + chr7.2:72991860-72991879 MS.gene79960:CDS 30.0%
TGTTGAGCTTAAAGATTTCT+TGG + chr7.2:72992016-72992035 MS.gene79960:CDS 30.0%
TTACTCTCAATCTCAAAATC+AGG + chr7.2:72992082-72992101 MS.gene79960:CDS 30.0%
TTGTTTGAGAATTAGGATGT+TGG - chr7.2:72991488-72991507 None:intergenic 30.0%
! AGTGTTCTGTTGATAGAATT+TGG + chr7.2:72991814-72991833 MS.gene79960:CDS 30.0%
! GTTAAAGGTATAAGATTTGG+TGG + chr7.2:72991549-72991568 MS.gene79960:CDS 30.0%
! TCTTATACCTTTAACTTCTG+AGG - chr7.2:72991544-72991563 None:intergenic 30.0%
!! AGAGGTTTTGCTTTTCTTAT+TGG - chr7.2:72991597-72991616 None:intergenic 30.0%
!!! GGAAGTTTTTTGTTGTCTTT+TGG - chr7.2:72991660-72991679 None:intergenic 30.0%
!!! TTGTCTTTTGGTTTTGTTGT+TGG - chr7.2:72991648-72991667 None:intergenic 30.0%
AAACCAACCTCAGAAGTTAA+AGG + chr7.2:72991534-72991553 MS.gene79960:CDS 35.0%
AAACTCATAAAGGGTCTTTG+TGG + chr7.2:72991870-72991889 MS.gene79960:CDS 35.0%
AAATGGCTAGGTTCAAATTC+AGG + chr7.2:72991898-72991917 MS.gene79960:CDS 35.0%
GCTAGGATTGTGAAATTTGT+TGG - chr7.2:72991708-72991727 None:intergenic 35.0%
GTGTATGCTGTTAGAGAAAT+TGG + chr7.2:72991942-72991961 MS.gene79960:CDS 35.0%
GTTAGAGAAATTGGAAGCTT+TGG + chr7.2:72991951-72991970 MS.gene79960:CDS 35.0%
! AGAACTTTGAGAAGTTCAAG+AGG - chr7.2:72991615-72991634 None:intergenic 35.0%
! ATCTCAAAATCAGGTTGCTA+TGG + chr7.2:72992091-72992110 MS.gene79960:CDS 35.0%
! TGTGTTTGTGTTTGAAACTG+AGG + chr7.2:72991785-72991804 MS.gene79960:CDS 35.0%
!! ATACCTTTAACTTCTGAGGT+TGG - chr7.2:72991540-72991559 None:intergenic 35.0%
!!! CTTTTGGTTTTGTTGTTGGT+GGG - chr7.2:72991644-72991663 None:intergenic 35.0%
!!! TCTTTTGGTTTTGTTGTTGG+TGG - chr7.2:72991645-72991664 None:intergenic 35.0%
!!! TTTGTGGTTTTGAAATGGCT+AGG + chr7.2:72991886-72991905 MS.gene79960:CDS 35.0%
AATGCTGTTGTTGAAGGGAA+AGG - chr7.2:72991681-72991700 None:intergenic 40.0%
ATTTCTTTGATCCAAGCCCT+AGG - chr7.2:72991755-72991774 None:intergenic 40.0%
GCAAGAATGCTGTTGTTGAA+GGG - chr7.2:72991686-72991705 None:intergenic 40.0%
GCTAGGTTCAAATTCAGGAA+AGG + chr7.2:72991903-72991922 MS.gene79960:CDS 40.0%
TGGTTTGAAGGAGAAATCTG+AGG - chr7.2:72991520-72991539 None:intergenic 40.0%
TTTCTTTGATCCAAGCCCTA+GGG - chr7.2:72991754-72991773 None:intergenic 40.0%
!! AACTTCTGAGGTTGGTTTGA+AGG - chr7.2:72991532-72991551 None:intergenic 40.0%
!! TCACCAAGTGCTATTTCATG+TGG - chr7.2:72991840-72991859 None:intergenic 40.0%
!!! GGGTCTTTGTGGTTTTGAAA+TGG + chr7.2:72991881-72991900 MS.gene79960:CDS 40.0%
CAATCCTAGCACATCAAGCA+TGG + chr7.2:72991718-72991737 MS.gene79960:CDS 45.0%
GGCAAGAATGCTGTTGTTGA+AGG - chr7.2:72991687-72991706 None:intergenic 45.0%
TGGCCACATGAAATAGCACT+TGG + chr7.2:72991834-72991853 MS.gene79960:CDS 45.0%
CATGGCACACACTAACCCTA+GGG + chr7.2:72991736-72991755 MS.gene79960:CDS 50.0%
TGTGCCATGCTTGATGTGCT+AGG - chr7.2:72991725-72991744 None:intergenic 50.0%
CACACACTAACCCTAGGGCT+TGG + chr7.2:72991741-72991760 MS.gene79960:CDS 55.0%
GCATGGCACACACTAACCCT+AGG + chr7.2:72991735-72991754 MS.gene79960:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 72991438 72992121 72991438 ID=MS.gene79960
chr7.2 mRNA 72991438 72992121 72991438 ID=MS.gene79960.t1;Parent=MS.gene79960
chr7.2 exon 72991438 72992121 72991438 ID=MS.gene79960.t1.exon1;Parent=MS.gene79960.t1
chr7.2 CDS 72991438 72992121 72991438 ID=cds.MS.gene79960.t1;Parent=MS.gene79960.t1
Gene Sequence

>MS.gene79960

ATGGGAGAAAAAGAGAAGAACAAGAACAATAACAAGAAACAAAAACACCAACATCCTAATTCTCAAACAACAAAACAAACCTCAGATTTCTCCTTCAAACCAACCTCAGAAGTTAAAGGTATAAGATTTGGTGGCCAATTCATAGTAAAATCATTTCCAATAAGAAAAGCAAAACCTCTTGAACTTCTCAAAGTTCTTTCTTTCCCACCAACAACAAAACCAAAAGACAACAAAAAACTTCCTTTCCCTTCAACAACAGCATTCTTGCCAACAAATTTCACAATCCTAGCACATCAAGCATGGCACACACTAACCCTAGGGCTTGGATCAAAGAAATCAAAAGTTCTTGTGTTTGTGTTTGAAACTGAGGCTATGAAGTGTTCTGTTGATAGAATTTGGCCACATGAAATAGCACTTGGTGATGTGAACAAGAAACTCATAAAGGGTCTTTGTGGTTTTGAAATGGCTAGGTTCAAATTCAGGAAAGGTTGTTTGACTTTTTATGTGTATGCTGTTAGAGAAATTGGAAGCTTTGGTTTTCTTTGTGCTGAAGATTTGAAGACTATTCTTCAATCTGTTGTTGAGCTTAAAGATTTCTTGGATCATACTGTTATGCTTGCTATGCCAAATCAAAGAAGTATTAATTACTCTCAATCTCAAAATCAGGTTGCTATGGCTCATTAA

Protein sequence

>MS.gene79960.t1

MGEKEKNKNNNKKQKHQHPNSQTTKQTSDFSFKPTSEVKGIRFGGQFIVKSFPIRKAKPLELLKVLSFPPTTKPKDNKKLPFPSTTAFLPTNFTILAHQAWHTLTLGLGSKKSKVLVFVFETEAMKCSVDRIWPHEIALGDVNKKLIKGLCGFEMARFKFRKGCLTFYVYAVREIGSFGFLCAEDLKTILQSVVELKDFLDHTVMLAMPNQRSINYSQSQNQVAMAH