Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80744.t1 | XP_024636682.1 | 96.9 | 295 | 9 | 0 | 1 | 295 | 16 | 310 | 4.30E-149 | 537.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80744.t1 | O80942 | 31.0 | 294 | 183 | 6 | 13 | 291 | 10 | 298 | 4.6e-22 | 106.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80744.t1 | A0A396I621 | 96.9 | 295 | 9 | 0 | 1 | 295 | 16 | 310 | 3.1e-149 | 537.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene80744 | -0.811944 | 5.43E-51 | -1.69E-46 |
| MS.gene049222 | MS.gene80744 | -0.80485 | 1.77E-49 | -1.69E-46 |
| MS.gene04932 | MS.gene80744 | 0.868538 | 6.06E-66 | -1.69E-46 |
| MS.gene049549 | MS.gene80744 | 0.905601 | 3.61E-80 | -1.69E-46 |
| MS.gene049609 | MS.gene80744 | -0.809431 | 1.90E-50 | -1.69E-46 |
| MS.gene049611 | MS.gene80744 | 0.805056 | 1.60E-49 | -1.69E-46 |
| MS.gene049634 | MS.gene80744 | 0.815498 | 8.97E-52 | -1.69E-46 |
| MS.gene049761 | MS.gene80744 | 0.800533 | 1.38E-48 | -1.69E-46 |
| MS.gene050095 | MS.gene80744 | 0.813121 | 3.00E-51 | -1.69E-46 |
| MS.gene050096 | MS.gene80744 | 0.822337 | 2.50E-53 | -1.69E-46 |
| MS.gene050170 | MS.gene80744 | -0.803311 | 3.70E-49 | -1.69E-46 |
| MS.gene050329 | MS.gene80744 | 0.818024 | 2.43E-52 | -1.69E-46 |
| MS.gene050380 | MS.gene80744 | 0.801783 | 7.65E-49 | -1.69E-46 |
| MS.gene050593 | MS.gene80744 | -0.801039 | 1.09E-48 | -1.69E-46 |
| MS.gene050647 | MS.gene80744 | 0.835855 | 1.32E-56 | -1.69E-46 |
| MS.gene050720 | MS.gene80744 | 0.81187 | 5.64E-51 | -1.69E-46 |
| MS.gene050772 | MS.gene80744 | -0.81288 | 3.39E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene80744 | -0.806613 | 7.56E-50 | -1.69E-46 |
| MS.gene050871 | MS.gene80744 | -0.805321 | 1.41E-49 | -1.69E-46 |
| MS.gene05104 | MS.gene80744 | 0.806291 | 8.83E-50 | -1.69E-46 |
| MS.gene051127 | MS.gene80744 | 0.820371 | 7.11E-53 | -1.69E-46 |
| MS.gene051131 | MS.gene80744 | 0.866667 | 2.41E-65 | -1.69E-46 |
| MS.gene051138 | MS.gene80744 | 0.810045 | 1.40E-50 | -1.69E-46 |
| MS.gene051158 | MS.gene80744 | 0.817413 | 3.34E-52 | -1.69E-46 |
| MS.gene051385 | MS.gene80744 | 0.802766 | 4.80E-49 | -1.69E-46 |
| MS.gene051440 | MS.gene80744 | 0.826382 | 2.80E-54 | -1.69E-46 |
| MS.gene051632 | MS.gene80744 | 0.800616 | 1.33E-48 | -1.69E-46 |
| MS.gene051844 | MS.gene80744 | 0.829867 | 4.05E-55 | -1.69E-46 |
| MS.gene051996 | MS.gene80744 | 0.823304 | 1.49E-53 | -1.69E-46 |
| MS.gene052134 | MS.gene80744 | -0.807095 | 5.97E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene80744 | 0.908576 | 1.47E-81 | -1.69E-46 |
| MS.gene052212 | MS.gene80744 | 0.855627 | 5.56E-62 | -1.69E-46 |
| MS.gene052223 | MS.gene80744 | 0.808542 | 2.94E-50 | -1.69E-46 |
| MS.gene05240 | MS.gene80744 | 0.865715 | 4.83E-65 | -1.69E-46 |
| MS.gene052414 | MS.gene80744 | -0.804362 | 2.24E-49 | -1.69E-46 |
| MS.gene05244 | MS.gene80744 | 0.854754 | 9.97E-62 | -1.69E-46 |
| MS.gene052501 | MS.gene80744 | -0.819791 | 9.65E-53 | -1.69E-46 |
| MS.gene052503 | MS.gene80744 | -0.811312 | 7.46E-51 | -1.69E-46 |
| MS.gene052505 | MS.gene80744 | -0.818817 | 1.61E-52 | -1.69E-46 |
| MS.gene052606 | MS.gene80744 | 0.863975 | 1.70E-64 | -1.69E-46 |
| MS.gene052702 | MS.gene80744 | 0.803436 | 3.49E-49 | -1.69E-46 |
| MS.gene052705 | MS.gene80744 | 0.816437 | 5.53E-52 | -1.69E-46 |
| MS.gene052706 | MS.gene80744 | 0.812973 | 3.24E-51 | -1.69E-46 |
| MS.gene052823 | MS.gene80744 | -0.814118 | 1.81E-51 | -1.69E-46 |
| MS.gene052825 | MS.gene80744 | -0.810875 | 9.27E-51 | -1.69E-46 |
| MS.gene053403 | MS.gene80744 | 0.855312 | 6.86E-62 | -1.69E-46 |
| MS.gene053406 | MS.gene80744 | 0.875794 | 2.33E-68 | -1.69E-46 |
| MS.gene053420 | MS.gene80744 | 0.83716 | 6.12E-57 | -1.69E-46 |
| MS.gene053449 | MS.gene80744 | -0.80257 | 5.27E-49 | -1.69E-46 |
| MS.gene053479 | MS.gene80744 | -0.806696 | 7.26E-50 | -1.69E-46 |
| MS.gene053488 | MS.gene80744 | 0.810817 | 9.54E-51 | -1.69E-46 |
| MS.gene053574 | MS.gene80744 | -0.810382 | 1.19E-50 | -1.69E-46 |
| MS.gene053824 | MS.gene80744 | -0.803079 | 4.14E-49 | -1.69E-46 |
| MS.gene053887 | MS.gene80744 | 0.843528 | 1.33E-58 | -1.69E-46 |
| MS.gene053916 | MS.gene80744 | -0.80437 | 2.23E-49 | -1.69E-46 |
| MS.gene054120 | MS.gene80744 | 0.831071 | 2.05E-55 | -1.69E-46 |
| MS.gene054138 | MS.gene80744 | 0.821372 | 4.18E-53 | -1.69E-46 |
| MS.gene054146 | MS.gene80744 | 0.909828 | 3.70E-82 | -1.69E-46 |
| MS.gene054147 | MS.gene80744 | 0.829574 | 4.77E-55 | -1.69E-46 |
| MS.gene054181 | MS.gene80744 | 0.833107 | 6.45E-56 | -1.69E-46 |
| MS.gene054277 | MS.gene80744 | 0.857426 | 1.65E-62 | -1.69E-46 |
| MS.gene054290 | MS.gene80744 | 0.813995 | 1.93E-51 | -1.69E-46 |
| MS.gene054421 | MS.gene80744 | 0.832884 | 7.33E-56 | -1.69E-46 |
| MS.gene054706 | MS.gene80744 | 0.819536 | 1.10E-52 | -1.69E-46 |
| MS.gene054707 | MS.gene80744 | 0.802369 | 5.80E-49 | -1.69E-46 |
| MS.gene05470 | MS.gene80744 | -0.804764 | 1.85E-49 | -1.69E-46 |
| MS.gene054742 | MS.gene80744 | 0.888298 | 6.68E-73 | -1.69E-46 |
| MS.gene05483 | MS.gene80744 | 0.802192 | 6.30E-49 | -1.69E-46 |
| MS.gene054884 | MS.gene80744 | 0.807011 | 6.22E-50 | -1.69E-46 |
| MS.gene055124 | MS.gene80744 | 0.82467 | 7.12E-54 | -1.69E-46 |
| MS.gene055154 | MS.gene80744 | 0.828505 | 8.66E-55 | -1.69E-46 |
| MS.gene055202 | MS.gene80744 | 0.803394 | 3.56E-49 | -1.69E-46 |
| MS.gene055240 | MS.gene80744 | 0.904474 | 1.18E-79 | -1.69E-46 |
| MS.gene055289 | MS.gene80744 | -0.8181 | 2.34E-52 | -1.69E-46 |
| MS.gene055340 | MS.gene80744 | 0.829642 | 4.59E-55 | -1.69E-46 |
| MS.gene055502 | MS.gene80744 | -0.803534 | 3.33E-49 | -1.69E-46 |
| MS.gene055601 | MS.gene80744 | 0.802591 | 5.22E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene80744 | -0.813134 | 2.98E-51 | -1.69E-46 |
| MS.gene056076 | MS.gene80744 | 0.818693 | 1.72E-52 | -1.69E-46 |
| MS.gene056218 | MS.gene80744 | 0.845409 | 4.14E-59 | -1.69E-46 |
| MS.gene056224 | MS.gene80744 | 0.83929 | 1.73E-57 | -1.69E-46 |
| MS.gene056298 | MS.gene80744 | 0.812768 | 3.59E-51 | -1.69E-46 |
| MS.gene056330 | MS.gene80744 | 0.86023 | 2.39E-63 | -1.69E-46 |
| MS.gene056387 | MS.gene80744 | -0.801825 | 7.50E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene80744 | -0.809914 | 1.49E-50 | -1.69E-46 |
| MS.gene056514 | MS.gene80744 | -0.800054 | 1.73E-48 | -1.69E-46 |
| MS.gene056590 | MS.gene80744 | 0.872167 | 3.91E-67 | -1.69E-46 |
| MS.gene056591 | MS.gene80744 | 0.870055 | 1.95E-66 | -1.69E-46 |
| MS.gene056680 | MS.gene80744 | 0.819615 | 1.06E-52 | -1.69E-46 |
| MS.gene056697 | MS.gene80744 | 0.806853 | 6.72E-50 | -1.69E-46 |
| MS.gene056714 | MS.gene80744 | 0.809682 | 1.68E-50 | -1.69E-46 |
| MS.gene056715 | MS.gene80744 | 0.8326 | 8.62E-56 | -1.69E-46 |
| MS.gene056748 | MS.gene80744 | 0.84835 | 6.48E-60 | -1.69E-46 |
| MS.gene056920 | MS.gene80744 | 0.872525 | 2.97E-67 | -1.69E-46 |
| MS.gene056922 | MS.gene80744 | 0.843278 | 1.55E-58 | -1.69E-46 |
| MS.gene056923 | MS.gene80744 | 0.831551 | 1.57E-55 | -1.69E-46 |
| MS.gene056924 | MS.gene80744 | 0.818053 | 2.40E-52 | -1.69E-46 |
| MS.gene057048 | MS.gene80744 | -0.833646 | 4.73E-56 | -1.69E-46 |
| MS.gene057261 | MS.gene80744 | 0.827395 | 1.60E-54 | -1.69E-46 |
| MS.gene057544 | MS.gene80744 | 0.834625 | 2.69E-56 | -1.69E-46 |
| MS.gene057547 | MS.gene80744 | 0.823343 | 1.46E-53 | -1.69E-46 |
| MS.gene057550 | MS.gene80744 | 0.826306 | 2.92E-54 | -1.69E-46 |
| MS.gene057768 | MS.gene80744 | 0.824448 | 8.03E-54 | -1.69E-46 |
| MS.gene057820 | MS.gene80744 | 0.816267 | 6.04E-52 | -1.69E-46 |
| MS.gene058067 | MS.gene80744 | 0.800706 | 1.27E-48 | -1.69E-46 |
| MS.gene058068 | MS.gene80744 | 0.802051 | 6.74E-49 | -1.69E-46 |
| MS.gene058093 | MS.gene80744 | 0.804507 | 2.09E-49 | -1.69E-46 |
| MS.gene058099 | MS.gene80744 | 0.808737 | 2.67E-50 | -1.69E-46 |
| MS.gene058109 | MS.gene80744 | 0.801034 | 1.09E-48 | -1.69E-46 |
| MS.gene058117 | MS.gene80744 | 0.803642 | 3.16E-49 | -1.69E-46 |
| MS.gene058171 | MS.gene80744 | 0.800654 | 1.30E-48 | -1.69E-46 |
| MS.gene058175 | MS.gene80744 | 0.80403 | 2.63E-49 | -1.69E-46 |
| MS.gene058177 | MS.gene80744 | 0.830482 | 2.87E-55 | -1.69E-46 |
| MS.gene058218 | MS.gene80744 | 0.80044 | 1.44E-48 | -1.69E-46 |
| MS.gene05837 | MS.gene80744 | -0.823508 | 1.33E-53 | -1.69E-46 |
| MS.gene058381 | MS.gene80744 | 0.837432 | 5.22E-57 | -1.69E-46 |
| MS.gene058418 | MS.gene80744 | 0.80754 | 4.81E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene80744 | -0.800841 | 1.19E-48 | -1.69E-46 |
| MS.gene058450 | MS.gene80744 | 0.824455 | 8.00E-54 | -1.69E-46 |
| MS.gene058603 | MS.gene80744 | 0.841957 | 3.47E-58 | -1.69E-46 |
| MS.gene058838 | MS.gene80744 | -0.808766 | 2.64E-50 | -1.69E-46 |
| MS.gene059037 | MS.gene80744 | 0.805815 | 1.11E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene80744 | 0.819392 | 1.19E-52 | -1.69E-46 |
| MS.gene059328 | MS.gene80744 | 0.823255 | 1.53E-53 | -1.69E-46 |
| MS.gene05934 | MS.gene80744 | -0.800287 | 1.55E-48 | -1.69E-46 |
| MS.gene059402 | MS.gene80744 | 0.830739 | 2.48E-55 | -1.69E-46 |
| MS.gene059892 | MS.gene80744 | 0.813085 | 3.06E-51 | -1.69E-46 |
| MS.gene059902 | MS.gene80744 | -0.803981 | 2.69E-49 | -1.69E-46 |
| MS.gene059953 | MS.gene80744 | 0.847814 | 9.11E-60 | -1.69E-46 |
| MS.gene059955 | MS.gene80744 | 0.873904 | 1.02E-67 | -1.69E-46 |
| MS.gene060087 | MS.gene80744 | 0.875722 | 2.46E-68 | -1.69E-46 |
| MS.gene060358 | MS.gene80744 | 0.810873 | 9.28E-51 | -1.69E-46 |
| MS.gene060372 | MS.gene80744 | -0.810898 | 9.17E-51 | -1.69E-46 |
| MS.gene060405 | MS.gene80744 | 0.811602 | 6.45E-51 | -1.69E-46 |
| MS.gene060593 | MS.gene80744 | 0.823885 | 1.09E-53 | -1.69E-46 |
| MS.gene06077 | MS.gene80744 | -0.803947 | 2.73E-49 | -1.69E-46 |
| MS.gene060835 | MS.gene80744 | -0.8048 | 1.81E-49 | -1.69E-46 |
| MS.gene061038 | MS.gene80744 | 0.80393 | 2.76E-49 | -1.69E-46 |
| MS.gene061092 | MS.gene80744 | 0.82979 | 4.23E-55 | -1.69E-46 |
| MS.gene061094 | MS.gene80744 | 0.832831 | 7.55E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80744.t1 | MTR_4g021270 | 96.939 | 294 | 9 | 0 | 1 | 294 | 16 | 309 | 0.0 | 596 |
| MS.gene80744.t1 | MTR_3g068095 | 34.495 | 287 | 106 | 11 | 21 | 290 | 33 | 254 | 4.34e-32 | 119 |
| MS.gene80744.t1 | MTR_5g080660 | 54.737 | 95 | 36 | 2 | 20 | 107 | 23 | 117 | 7.11e-28 | 108 |
| MS.gene80744.t1 | MTR_5g014400 | 40.678 | 177 | 67 | 9 | 20 | 190 | 58 | 202 | 1.33e-26 | 105 |
| MS.gene80744.t1 | MTR_2g076060 | 79.070 | 43 | 8 | 1 | 36 | 77 | 41 | 83 | 2.58e-19 | 85.1 |
| MS.gene80744.t1 | MTR_1g070265 | 82.051 | 39 | 7 | 0 | 38 | 76 | 29 | 67 | 3.37e-17 | 78.2 |
| MS.gene80744.t1 | MTR_7g063220 | 66.667 | 42 | 14 | 0 | 44 | 85 | 24 | 65 | 3.59e-14 | 68.6 |
| MS.gene80744.t1 | MTR_4g064873 | 75.000 | 36 | 9 | 0 | 43 | 78 | 20 | 55 | 6.17e-14 | 68.9 |
| MS.gene80744.t1 | MTR_1g075430 | 69.444 | 36 | 11 | 0 | 44 | 79 | 18 | 53 | 4.95e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80744.t1 | AT2G37740 | 31.186 | 295 | 181 | 6 | 13 | 291 | 10 | 298 | 3.91e-28 | 110 |
| MS.gene80744.t1 | AT5G06070 | 65.333 | 75 | 24 | 1 | 6 | 78 | 13 | 87 | 8.81e-26 | 102 |
| MS.gene80744.t1 | AT4G17810 | 70.370 | 54 | 15 | 1 | 20 | 73 | 21 | 73 | 1.22e-21 | 90.1 |
| MS.gene80744.t1 | AT4G17810 | 70.370 | 54 | 15 | 1 | 20 | 73 | 21 | 73 | 1.39e-21 | 90.5 |
| MS.gene80744.t1 | AT2G42410 | 67.273 | 55 | 15 | 1 | 37 | 91 | 40 | 91 | 1.78e-19 | 85.1 |
| MS.gene80744.t1 | AT2G42410 | 67.273 | 55 | 15 | 1 | 37 | 91 | 40 | 91 | 1.78e-19 | 85.1 |
| MS.gene80744.t1 | AT2G42410 | 67.273 | 55 | 15 | 1 | 37 | 91 | 66 | 117 | 2.24e-19 | 85.5 |
| MS.gene80744.t1 | AT3G23130 | 81.395 | 43 | 8 | 0 | 36 | 78 | 37 | 79 | 1.20e-18 | 82.8 |
| MS.gene80744.t1 | AT3G09290 | 66.667 | 39 | 13 | 0 | 44 | 82 | 33 | 71 | 5.13e-12 | 63.5 |
Find 56 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAGTTGAAAGGAGAAATT+AGG | 0.144993 | 4.4:-80589735 | None:intergenic |
| TCTACCTCCTCAATTGAATT+AGG | 0.148497 | 4.4:-80590087 | None:intergenic |
| AAACCACAAGTAGGAGAATT+AGG | 0.187380 | 4.4:-80589613 | None:intergenic |
| AAATGATCAAGATGAGAATT+AGG | 0.200848 | 4.4:-80589655 | None:intergenic |
| ATCACCTTCGTCGGCTTCTA+AGG | 0.266590 | 4.4:+80589693 | MS.gene80744:CDS |
| AACCCTTCTTCTTCTTGTTA+TGG | 0.304635 | 4.4:+80589292 | MS.gene80744:CDS |
| CCAAAGGTATAATAGATTCT+AGG | 0.312053 | 4.4:+80589845 | MS.gene80744:CDS |
| AGAGTTGAAAGGAGAAATTA+GGG | 0.331194 | 4.4:-80589734 | None:intergenic |
| TAATTCTCCTACTTGTGGTT+TGG | 0.335006 | 4.4:+80589615 | MS.gene80744:CDS |
| CTTCTTGTTATGGTGATTCA+TGG | 0.338824 | 4.4:+80589302 | MS.gene80744:CDS |
| CTACCTCCTCAATTGAATTA+GGG | 0.346783 | 4.4:-80590086 | None:intergenic |
| TGTAGCTATGAGCTTGAATT+TGG | 0.353912 | 4.4:+80589903 | MS.gene80744:CDS |
| TTTAGGTCAGCACAAGCATT+AGG | 0.361635 | 4.4:+80589421 | MS.gene80744:CDS |
| CCTAGAATCTATTATACCTT+TGG | 0.378017 | 4.4:-80589845 | None:intergenic |
| TCGGCTTCTAAGGCTTTATT+CGG | 0.401138 | 4.4:+80589703 | MS.gene80744:CDS |
| CTTTCAACTCTTCTGAATCA+TGG | 0.401376 | 4.4:+80589746 | MS.gene80744:CDS |
| TTCAATTGAGGAGGTAGATC+TGG | 0.403141 | 4.4:+80590092 | MS.gene80744:CDS |
| GAACATAATATATCATTTCC+AGG | 0.405382 | 4.4:-80589778 | None:intergenic |
| AATGATCAAGATGAGAATTA+GGG | 0.405455 | 4.4:-80589654 | None:intergenic |
| GATTCTAGGTTTCGAGGTAA+AGG | 0.408364 | 4.4:+80589859 | MS.gene80744:CDS |
| ATTCTAGGTTTCGAGGTAAA+GGG | 0.409155 | 4.4:+80589860 | MS.gene80744:CDS |
| AGTTAGTGAACTAGCAAGAT+TGG | 0.417575 | 4.4:-80589269 | None:intergenic |
| GATGCAGCTGCAGGGTCACT+TGG | 0.420108 | 4.4:+80589346 | MS.gene80744:CDS |
| TTTGCTGAAGATGCAGCTGC+AGG | 0.424497 | 4.4:+80589337 | MS.gene80744:CDS |
| AACCACAAGTAGGAGAATTA+GGG | 0.432833 | 4.4:-80589612 | None:intergenic |
| AAGTAGGAGAATTAGGGAAT+AGG | 0.457191 | 4.4:-80589606 | None:intergenic |
| AAGCTACAAGAATATGATCT+TGG | 0.459400 | 4.4:-80589385 | None:intergenic |
| TTCCCTAATTCTCCTACTTG+TGG | 0.462438 | 4.4:+80589610 | MS.gene80744:CDS |
| AGGAGGTAGATCTGGAGTTA+AGG | 0.476987 | 4.4:+80590100 | MS.gene80744:CDS |
| GTTCAACCCTAATTCAATTG+AGG | 0.483460 | 4.4:+80590080 | MS.gene80744:CDS |
| TTCTTGTTATGGTGATTCAT+GGG | 0.486889 | 4.4:+80589303 | MS.gene80744:CDS |
| AAGATTTCAATTGCAAGAAG+AGG | 0.493658 | 4.4:+80589974 | MS.gene80744:CDS |
| TTCAGCTTCATCACCTTCGT+CGG | 0.494636 | 4.4:+80589684 | MS.gene80744:CDS |
| CGATTTCAAGCTCGTGATGA+TGG | 0.497652 | 4.4:-80589534 | None:intergenic |
| ATGCAGCTGCAGGGTCACTT+GGG | 0.499136 | 4.4:+80589347 | MS.gene80744:CDS |
| GAATCATGGCTGAATTTACC+TGG | 0.500114 | 4.4:+80589760 | MS.gene80744:CDS |
| GTACTCTCAAATTCAACAAG+TGG | 0.518666 | 4.4:-80589943 | None:intergenic |
| TCACCATAACAAGAAGAAGA+AGG | 0.520396 | 4.4:-80589295 | None:intergenic |
| TATGCTACTTAACTATACCT+TGG | 0.528071 | 4.4:-80590136 | None:intergenic |
| CTACAAGAATATGATCTTGG+TGG | 0.544295 | 4.4:-80589382 | None:intergenic |
| TGGAGTTAAGGCTTGGTACA+AGG | 0.549906 | 4.4:+80590112 | MS.gene80744:CDS |
| TGCAGCTGCAGGGTCACTTG+GGG | 0.554361 | 4.4:+80589348 | MS.gene80744:CDS |
| TTGCTGAAGATGCAGCTGCA+GGG | 0.559256 | 4.4:+80589338 | MS.gene80744:CDS |
| CATGATTCAGAAGAGTTGAA+AGG | 0.568556 | 4.4:-80589745 | None:intergenic |
| GTAGATCTGGAGTTAAGGCT+TGG | 0.574970 | 4.4:+80590105 | MS.gene80744:CDS |
| TTTGATCATAAAGTCTCCAA+AGG | 0.581337 | 4.4:+80589829 | MS.gene80744:CDS |
| ATAATAGATTCTAGGTTTCG+AGG | 0.582395 | 4.4:+80589853 | MS.gene80744:CDS |
| CACCATAACAAGAAGAAGAA+GGG | 0.582469 | 4.4:-80589294 | None:intergenic |
| TTCTAGGTTTCGAGGTAAAG+GGG | 0.591482 | 4.4:+80589861 | MS.gene80744:CDS |
| CAACCCTAATTCAATTGAGG+AGG | 0.592265 | 4.4:+80590083 | MS.gene80744:CDS |
| TTATAAACCAAACCACAAGT+AGG | 0.601660 | 4.4:-80589622 | None:intergenic |
| TCTAGGTTTCGAGGTAAAGG+GGG | 0.621509 | 4.4:+80589862 | MS.gene80744:CDS |
| GCAGCTGCAGGGTCACTTGG+GGG | 0.624450 | 4.4:+80589349 | MS.gene80744:CDS |
| AAGGCTTGGTACAAGGTCCA+AGG | 0.631211 | 4.4:+80590119 | MS.gene80744:CDS |
| AGGTCAGCACAAGCATTAGG+TGG | 0.642240 | 4.4:+80589424 | MS.gene80744:CDS |
| AAAGCCTTAGAAGCCGACGA+AGG | 0.711061 | 4.4:-80589697 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATATTTTGTTGTTTATCAA+AGG | - | chr4.4:80590043-80590062 | None:intergenic | 20.0% |
| !!! | TTATAAACATTTTGAATGAG+TGG | - | chr4.4:80589580-80589599 | None:intergenic | 20.0% |
| ! | AAATGATCAAGATGAGAATT+AGG | - | chr4.4:80589658-80589677 | None:intergenic | 25.0% |
| ! | AATGATCAAGATGAGAATTA+GGG | - | chr4.4:80589657-80589676 | None:intergenic | 25.0% |
| ! | GAACATAATATATCATTTCC+AGG | - | chr4.4:80589781-80589800 | None:intergenic | 25.0% |
| !! | TAAAGAATCTGATCATTTTG+TGG | - | chr4.4:80589496-80589515 | None:intergenic | 25.0% |
| AAGAGTTGAAAGGAGAAATT+AGG | - | chr4.4:80589738-80589757 | None:intergenic | 30.0% | |
| AAGATTTCAATTGCAAGAAG+AGG | + | chr4.4:80589974-80589993 | MS.gene80744:CDS | 30.0% | |
| AAGCTACAAGAATATGATCT+TGG | - | chr4.4:80589388-80589407 | None:intergenic | 30.0% | |
| AGAGTTGAAAGGAGAAATTA+GGG | - | chr4.4:80589737-80589756 | None:intergenic | 30.0% | |
| ATAATAGATTCTAGGTTTCG+AGG | + | chr4.4:80589853-80589872 | MS.gene80744:CDS | 30.0% | |
| CCTAGAATCTATTATACCTT+TGG | - | chr4.4:80589848-80589867 | None:intergenic | 30.0% | |
| TTATAAACCAAACCACAAGT+AGG | - | chr4.4:80589625-80589644 | None:intergenic | 30.0% | |
| TTTGATCATAAAGTCTCCAA+AGG | + | chr4.4:80589829-80589848 | MS.gene80744:CDS | 30.0% | |
| ! | CCAAAGGTATAATAGATTCT+AGG | + | chr4.4:80589845-80589864 | MS.gene80744:CDS | 30.0% |
| ! | TTCTTGTTATGGTGATTCAT+GGG | + | chr4.4:80589303-80589322 | MS.gene80744:CDS | 30.0% |
| AAACCACAAGTAGGAGAATT+AGG | - | chr4.4:80589616-80589635 | None:intergenic | 35.0% | |
| AACCACAAGTAGGAGAATTA+GGG | - | chr4.4:80589615-80589634 | None:intergenic | 35.0% | |
| AACCCTTCTTCTTCTTGTTA+TGG | + | chr4.4:80589292-80589311 | MS.gene80744:CDS | 35.0% | |
| AAGTAGGAGAATTAGGGAAT+AGG | - | chr4.4:80589609-80589628 | None:intergenic | 35.0% | |
| AGTTAGTGAACTAGCAAGAT+TGG | - | chr4.4:80589272-80589291 | None:intergenic | 35.0% | |
| ATTCTAGGTTTCGAGGTAAA+GGG | + | chr4.4:80589860-80589879 | MS.gene80744:CDS | 35.0% | |
| CACCATAACAAGAAGAAGAA+GGG | - | chr4.4:80589297-80589316 | None:intergenic | 35.0% | |
| CATGATTCAGAAGAGTTGAA+AGG | - | chr4.4:80589748-80589767 | None:intergenic | 35.0% | |
| CTACAAGAATATGATCTTGG+TGG | - | chr4.4:80589385-80589404 | None:intergenic | 35.0% | |
| CTACCTCCTCAATTGAATTA+GGG | - | chr4.4:80590089-80590108 | None:intergenic | 35.0% | |
| CTTTCAACTCTTCTGAATCA+TGG | + | chr4.4:80589746-80589765 | MS.gene80744:CDS | 35.0% | |
| GTACTCTCAAATTCAACAAG+TGG | - | chr4.4:80589946-80589965 | None:intergenic | 35.0% | |
| GTTCAACCCTAATTCAATTG+AGG | + | chr4.4:80590080-80590099 | MS.gene80744:CDS | 35.0% | |
| TAATTCTCCTACTTGTGGTT+TGG | + | chr4.4:80589615-80589634 | MS.gene80744:CDS | 35.0% | |
| TCACCATAACAAGAAGAAGA+AGG | - | chr4.4:80589298-80589317 | None:intergenic | 35.0% | |
| TCTACCTCCTCAATTGAATT+AGG | - | chr4.4:80590090-80590109 | None:intergenic | 35.0% | |
| TGTAGCTATGAGCTTGAATT+TGG | + | chr4.4:80589903-80589922 | MS.gene80744:CDS | 35.0% | |
| ! | CTTCTTGTTATGGTGATTCA+TGG | + | chr4.4:80589302-80589321 | MS.gene80744:CDS | 35.0% |
| ! | GCTTTTGTAGAAGAGAGTTT+AGG | + | chr4.4:80589404-80589423 | MS.gene80744:CDS | 35.0% |
| CAACCCTAATTCAATTGAGG+AGG | + | chr4.4:80590083-80590102 | MS.gene80744:CDS | 40.0% | |
| GAATCATGGCTGAATTTACC+TGG | + | chr4.4:80589760-80589779 | MS.gene80744:CDS | 40.0% | |
| GATTCTAGGTTTCGAGGTAA+AGG | + | chr4.4:80589859-80589878 | MS.gene80744:CDS | 40.0% | |
| TTCAATTGAGGAGGTAGATC+TGG | + | chr4.4:80590092-80590111 | MS.gene80744:CDS | 40.0% | |
| TTCCCTAATTCTCCTACTTG+TGG | + | chr4.4:80589610-80589629 | MS.gene80744:CDS | 40.0% | |
| TTCTAGGTTTCGAGGTAAAG+GGG | + | chr4.4:80589861-80589880 | MS.gene80744:CDS | 40.0% | |
| TTTAGGTCAGCACAAGCATT+AGG | + | chr4.4:80589421-80589440 | MS.gene80744:CDS | 40.0% | |
| !! | TCGGCTTCTAAGGCTTTATT+CGG | + | chr4.4:80589703-80589722 | MS.gene80744:CDS | 40.0% |
| CGATTTCAAGCTCGTGATGA+TGG | - | chr4.4:80589537-80589556 | None:intergenic | 45.0% | |
| TCTAGGTTTCGAGGTAAAGG+GGG | + | chr4.4:80589862-80589881 | MS.gene80744:CDS | 45.0% | |
| TTCAGCTTCATCACCTTCGT+CGG | + | chr4.4:80589684-80589703 | MS.gene80744:CDS | 45.0% | |
| ! | AGGAGGTAGATCTGGAGTTA+AGG | + | chr4.4:80590100-80590119 | MS.gene80744:CDS | 45.0% |
| ! | GTAGATCTGGAGTTAAGGCT+TGG | + | chr4.4:80590105-80590124 | MS.gene80744:CDS | 45.0% |
| ! | TGGAGTTAAGGCTTGGTACA+AGG | + | chr4.4:80590112-80590131 | MS.gene80744:CDS | 45.0% |
| AAAGCCTTAGAAGCCGACGA+AGG | - | chr4.4:80589700-80589719 | None:intergenic | 50.0% | |
| AGGTCAGCACAAGCATTAGG+TGG | + | chr4.4:80589424-80589443 | MS.gene80744:CDS | 50.0% | |
| GGTCACTTGGGGGATGTATA+TGG | + | chr4.4:80589359-80589378 | MS.gene80744:CDS | 50.0% | |
| TTGCTGAAGATGCAGCTGCA+GGG | + | chr4.4:80589338-80589357 | MS.gene80744:CDS | 50.0% | |
| TTTGCTGAAGATGCAGCTGC+AGG | + | chr4.4:80589337-80589356 | MS.gene80744:CDS | 50.0% | |
| ! | AAGGCTTGGTACAAGGTCCA+AGG | + | chr4.4:80590119-80590138 | MS.gene80744:CDS | 50.0% |
| !! | ATCACCTTCGTCGGCTTCTA+AGG | + | chr4.4:80589693-80589712 | MS.gene80744:CDS | 50.0% |
| ATGCAGCTGCAGGGTCACTT+GGG | + | chr4.4:80589347-80589366 | MS.gene80744:CDS | 55.0% | |
| GATGCAGCTGCAGGGTCACT+TGG | + | chr4.4:80589346-80589365 | MS.gene80744:CDS | 60.0% | |
| TGCAGCTGCAGGGTCACTTG+GGG | + | chr4.4:80589348-80589367 | MS.gene80744:CDS | 60.0% | |
| GCAGCTGCAGGGTCACTTGG+GGG | + | chr4.4:80589349-80589368 | MS.gene80744:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 80589259 | 80590146 | 80589259 | ID=MS.gene80744 |
| chr4.4 | mRNA | 80589259 | 80590146 | 80589259 | ID=MS.gene80744.t1;Parent=MS.gene80744 |
| chr4.4 | exon | 80589259 | 80590146 | 80589259 | ID=MS.gene80744.t1.exon1;Parent=MS.gene80744.t1 |
| chr4.4 | CDS | 80589259 | 80590146 | 80589259 | ID=cds.MS.gene80744.t1;Parent=MS.gene80744.t1 |
| Gene Sequence |
| Protein sequence |
