AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073526


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073526.t1 MTR_4g021270 96.117 309 12 0 1 309 1 309 0.0 625
MS.gene073526.t1 MTR_3g068095 34.495 287 106 11 36 305 33 254 8.75e-32 119
MS.gene073526.t1 MTR_5g080660 46.825 126 45 4 4 122 7 117 5.83e-28 108
MS.gene073526.t1 MTR_5g014400 40.678 177 67 9 35 205 58 202 1.04e-26 105
MS.gene073526.t1 MTR_2g076060 79.070 43 8 1 51 92 41 83 4.61e-19 84.3
MS.gene073526.t1 MTR_1g070265 82.051 39 7 0 53 91 29 67 4.71e-17 78.2
MS.gene073526.t1 MTR_7g063220 66.667 42 14 0 59 100 24 65 4.28e-14 68.6
MS.gene073526.t1 MTR_4g064873 75.000 36 9 0 58 93 20 55 7.23e-14 68.9
MS.gene073526.t1 MTR_1g075430 69.444 36 11 0 59 94 18 53 6.31e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073526.t1 AT2G37740 31.186 295 181 7 28 306 10 298 5.39e-28 110
MS.gene073526.t1 AT5G06070 54.737 95 32 2 1 93 2 87 1.81e-25 102
MS.gene073526.t1 AT4G17810 70.370 54 15 1 35 88 21 73 2.13e-21 89.7
MS.gene073526.t1 AT4G17810 70.370 54 15 1 35 88 21 73 2.42e-21 90.1
MS.gene073526.t1 AT2G42410 67.273 55 15 1 52 106 40 91 2.60e-19 85.1
MS.gene073526.t1 AT2G42410 67.273 55 15 1 52 106 40 91 2.60e-19 85.1
MS.gene073526.t1 AT2G42410 67.273 55 15 1 52 106 66 117 3.59e-19 85.1
MS.gene073526.t1 AT3G23130 81.395 43 8 0 51 93 37 79 1.80e-18 82.4
MS.gene073526.t1 AT3G09290 66.667 39 13 0 59 97 33 71 6.34e-12 63.5

Find 58 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAGTTGAAAGGAGAAATT+AGG 0.144993 4.1:-76876018 None:intergenic
TCTACCTCCTCAATTGAATT+AGG 0.148497 4.1:-76876370 None:intergenic
AAATGATCAAGATGAGAATT+AGG 0.222386 4.1:-76875938 None:intergenic
ATCACCTTCGTCGGCTTCTA+AGG 0.266590 4.1:+76875976 MS.gene073526:CDS
AAACCACAAGTAGGAGAATT+AGG 0.289218 4.1:-76875896 None:intergenic
AACCCTTCTTCTTCTTGTTA+TGG 0.304635 4.1:+76875575 MS.gene073526:CDS
CCAAAGGTATAATAGATTCT+AGG 0.312053 4.1:+76876128 MS.gene073526:CDS
AGAGTTGAAAGGAGAAATTA+GGG 0.331194 4.1:-76876017 None:intergenic
CTTCTTGTTATGGTGATTCA+TGG 0.338824 4.1:+76875585 MS.gene073526:CDS
TAATTCTCCTACTTGTGGTT+TGG 0.343165 4.1:+76875898 MS.gene073526:CDS
CTACCTCCTCAATTGAATTA+GGG 0.346783 4.1:-76876369 None:intergenic
TGTAGCTATGAGCTTGAATT+TGG 0.353912 4.1:+76876186 MS.gene073526:CDS
AAGTAGGAGAATTAGGAAAT+AGG 0.358290 4.1:-76875889 None:intergenic
TTTAGGTCAGCACAAGCATT+AGG 0.361635 4.1:+76875704 MS.gene073526:CDS
CCTAGAATCTATTATACCTT+TGG 0.378017 4.1:-76876128 None:intergenic
TCGGCTTCTAAGGCTTTATT+CGG 0.401138 4.1:+76875986 MS.gene073526:CDS
CTTTCAACTCTTCTGAATCA+TGG 0.401376 4.1:+76876029 MS.gene073526:CDS
TTCAATTGAGGAGGTAGATC+TGG 0.403141 4.1:+76876375 MS.gene073526:CDS
GAACATAATATATCATTTCC+AGG 0.405382 4.1:-76876061 None:intergenic
GATTCTAGGTTTCGAGGTAA+AGG 0.408364 4.1:+76876142 MS.gene073526:CDS
ATTCTAGGTTTCGAGGTAAA+GGG 0.409155 4.1:+76876143 MS.gene073526:CDS
GATGCAGCTGCAGGGTCACT+TGG 0.420108 4.1:+76875629 MS.gene073526:CDS
TTATAAACATCTTGAATAAG+TGG 0.423507 4.1:-76875860 None:intergenic
TTTGCTGAAGATGCAGCTGC+AGG 0.424497 4.1:+76875620 MS.gene073526:CDS
GATCTTTGTATCAATGGAAC+AGG 0.433376 4.1:+76875484 None:intergenic
AATGATCAAGATGAGAATTA+GGG 0.444196 4.1:-76875937 None:intergenic
AAGCTACAAGAATATGATCT+TGG 0.459400 4.1:-76875668 None:intergenic
AGGAGGTAGATCTGGAGTTA+AGG 0.476987 4.1:+76876383 MS.gene073526:CDS
GTTCAACCCTAATTCAATTG+AGG 0.483460 4.1:+76876363 MS.gene073526:CDS
TTCTTGTTATGGTGATTCAT+GGG 0.486889 4.1:+76875586 MS.gene073526:CDS
AAGATTTCAATTGCAAGAAG+AGG 0.493658 4.1:+76876257 MS.gene073526:CDS
TTTCCTAATTCTCCTACTTG+TGG 0.494089 4.1:+76875893 MS.gene073526:CDS
ATGCAGCTGCAGGGTCACTT+GGG 0.499136 4.1:+76875630 MS.gene073526:CDS
GAATCATGGCTGAATTTACC+TGG 0.500114 4.1:+76876043 MS.gene073526:CDS
TTCAACTTCATCACCTTCGT+CGG 0.506895 4.1:+76875967 MS.gene073526:CDS
CTATTTCAAGCTCGTGATGA+TGG 0.509417 4.1:-76875817 None:intergenic
AATGGAACAGGATCAATGTT+GGG 0.518180 4.1:+76875496 None:intergenic
GTACTCTCAAATTCAACAAG+TGG 0.518666 4.1:-76876226 None:intergenic
TCACCATAACAAGAAGAAGA+AGG 0.520396 4.1:-76875578 None:intergenic
CAATGGAACAGGATCAATGT+TGG 0.521807 4.1:+76875495 None:intergenic
TATGCTACTTAACTATACCT+TGG 0.528071 4.1:-76876419 None:intergenic
CTACAAGAATATGATCTTGG+TGG 0.544295 4.1:-76875665 None:intergenic
TGGAGTTAAGGCTTGGTACA+AGG 0.549906 4.1:+76876395 MS.gene073526:CDS
TGCAGCTGCAGGGTCACTTG+GGG 0.554361 4.1:+76875631 MS.gene073526:CDS
TTGCTGAAGATGCAGCTGCA+GGG 0.559256 4.1:+76875621 MS.gene073526:CDS
CATGATTCAGAAGAGTTGAA+AGG 0.568556 4.1:-76876028 None:intergenic
GTAGATCTGGAGTTAAGGCT+TGG 0.574970 4.1:+76876388 MS.gene073526:CDS
TTTGATCATAAAGTCTCCAA+AGG 0.581337 4.1:+76876112 MS.gene073526:CDS
ATAATAGATTCTAGGTTTCG+AGG 0.582395 4.1:+76876136 MS.gene073526:CDS
CACCATAACAAGAAGAAGAA+GGG 0.582469 4.1:-76875577 None:intergenic
TTCTAGGTTTCGAGGTAAAG+GGG 0.591482 4.1:+76876144 MS.gene073526:CDS
CAACCCTAATTCAATTGAGG+AGG 0.592265 4.1:+76876366 MS.gene073526:CDS
TTATAAACCAAACCACAAGT+AGG 0.601660 4.1:-76875905 None:intergenic
TCTAGGTTTCGAGGTAAAGG+GGG 0.621509 4.1:+76876145 MS.gene073526:CDS
GCAGCTGCAGGGTCACTTGG+GGG 0.624450 4.1:+76875632 MS.gene073526:CDS
AAGGCTTGGTACAAGGTCCA+AGG 0.631211 4.1:+76876402 MS.gene073526:CDS
AGGTCAGCACAAGCATTAGG+TGG 0.642240 4.1:+76875707 MS.gene073526:CDS
AAAGCCTTAGAAGCCGACGA+AGG 0.711061 4.1:-76875980 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAAACATCTTGAATAAG+TGG - chr4.1:76875863-76875882 None:intergenic 20.0%
!!! CATATTTTGTTGTTTATCAA+AGG - chr4.1:76876326-76876345 None:intergenic 20.0%
! AAATGATCAAGATGAGAATT+AGG - chr4.1:76875941-76875960 None:intergenic 25.0%
! AATGATCAAGATGAGAATTA+GGG - chr4.1:76875940-76875959 None:intergenic 25.0%
! GAACATAATATATCATTTCC+AGG - chr4.1:76876064-76876083 None:intergenic 25.0%
!! TAAAGAATCTGATCATTTTG+TGG - chr4.1:76875779-76875798 None:intergenic 25.0%
AAGAGTTGAAAGGAGAAATT+AGG - chr4.1:76876021-76876040 None:intergenic 30.0%
AAGATTTCAATTGCAAGAAG+AGG + chr4.1:76876257-76876276 MS.gene073526:CDS 30.0%
AAGCTACAAGAATATGATCT+TGG - chr4.1:76875671-76875690 None:intergenic 30.0%
AAGTAGGAGAATTAGGAAAT+AGG - chr4.1:76875892-76875911 None:intergenic 30.0%
AGAGTTGAAAGGAGAAATTA+GGG - chr4.1:76876020-76876039 None:intergenic 30.0%
ATAATAGATTCTAGGTTTCG+AGG + chr4.1:76876136-76876155 MS.gene073526:CDS 30.0%
CCTAGAATCTATTATACCTT+TGG - chr4.1:76876131-76876150 None:intergenic 30.0%
TTATAAACCAAACCACAAGT+AGG - chr4.1:76875908-76875927 None:intergenic 30.0%
TTTGATCATAAAGTCTCCAA+AGG + chr4.1:76876112-76876131 MS.gene073526:CDS 30.0%
! CCAAAGGTATAATAGATTCT+AGG + chr4.1:76876128-76876147 MS.gene073526:CDS 30.0%
! TTCTTGTTATGGTGATTCAT+GGG + chr4.1:76875586-76875605 MS.gene073526:CDS 30.0%
AAACCACAAGTAGGAGAATT+AGG - chr4.1:76875899-76875918 None:intergenic 35.0%
AACCCTTCTTCTTCTTGTTA+TGG + chr4.1:76875575-76875594 MS.gene073526:CDS 35.0%
ATTCTAGGTTTCGAGGTAAA+GGG + chr4.1:76876143-76876162 MS.gene073526:CDS 35.0%
CACCATAACAAGAAGAAGAA+GGG - chr4.1:76875580-76875599 None:intergenic 35.0%
CATGATTCAGAAGAGTTGAA+AGG - chr4.1:76876031-76876050 None:intergenic 35.0%
CTACAAGAATATGATCTTGG+TGG - chr4.1:76875668-76875687 None:intergenic 35.0%
CTACCTCCTCAATTGAATTA+GGG - chr4.1:76876372-76876391 None:intergenic 35.0%
CTTTCAACTCTTCTGAATCA+TGG + chr4.1:76876029-76876048 MS.gene073526:CDS 35.0%
GTACTCTCAAATTCAACAAG+TGG - chr4.1:76876229-76876248 None:intergenic 35.0%
GTTCAACCCTAATTCAATTG+AGG + chr4.1:76876363-76876382 MS.gene073526:CDS 35.0%
TAATTCTCCTACTTGTGGTT+TGG + chr4.1:76875898-76875917 MS.gene073526:CDS 35.0%
TCACCATAACAAGAAGAAGA+AGG - chr4.1:76875581-76875600 None:intergenic 35.0%
TCTACCTCCTCAATTGAATT+AGG - chr4.1:76876373-76876392 None:intergenic 35.0%
TGTAGCTATGAGCTTGAATT+TGG + chr4.1:76876186-76876205 MS.gene073526:CDS 35.0%
TTTCCTAATTCTCCTACTTG+TGG + chr4.1:76875893-76875912 MS.gene073526:CDS 35.0%
! CTTCTTGTTATGGTGATTCA+TGG + chr4.1:76875585-76875604 MS.gene073526:CDS 35.0%
! GCTTTTGTAGAAGAGAGTTT+AGG + chr4.1:76875687-76875706 MS.gene073526:CDS 35.0%
CAACCCTAATTCAATTGAGG+AGG + chr4.1:76876366-76876385 MS.gene073526:CDS 40.0%
CTATTTCAAGCTCGTGATGA+TGG - chr4.1:76875820-76875839 None:intergenic 40.0%
GAATCATGGCTGAATTTACC+TGG + chr4.1:76876043-76876062 MS.gene073526:CDS 40.0%
GATTCTAGGTTTCGAGGTAA+AGG + chr4.1:76876142-76876161 MS.gene073526:CDS 40.0%
TTCAACTTCATCACCTTCGT+CGG + chr4.1:76875967-76875986 MS.gene073526:CDS 40.0%
TTCAATTGAGGAGGTAGATC+TGG + chr4.1:76876375-76876394 MS.gene073526:CDS 40.0%
TTCTAGGTTTCGAGGTAAAG+GGG + chr4.1:76876144-76876163 MS.gene073526:CDS 40.0%
TTTAGGTCAGCACAAGCATT+AGG + chr4.1:76875704-76875723 MS.gene073526:CDS 40.0%
!! TCGGCTTCTAAGGCTTTATT+CGG + chr4.1:76875986-76876005 MS.gene073526:CDS 40.0%
TCTAGGTTTCGAGGTAAAGG+GGG + chr4.1:76876145-76876164 MS.gene073526:CDS 45.0%
! AGGAGGTAGATCTGGAGTTA+AGG + chr4.1:76876383-76876402 MS.gene073526:CDS 45.0%
! GTAGATCTGGAGTTAAGGCT+TGG + chr4.1:76876388-76876407 MS.gene073526:CDS 45.0%
! TGGAGTTAAGGCTTGGTACA+AGG + chr4.1:76876395-76876414 MS.gene073526:CDS 45.0%
AAAGCCTTAGAAGCCGACGA+AGG - chr4.1:76875983-76876002 None:intergenic 50.0%
AGGTCAGCACAAGCATTAGG+TGG + chr4.1:76875707-76875726 MS.gene073526:CDS 50.0%
GGTCACTTGGGGGATGTATA+TGG + chr4.1:76875642-76875661 MS.gene073526:CDS 50.0%
TTGCTGAAGATGCAGCTGCA+GGG + chr4.1:76875621-76875640 MS.gene073526:CDS 50.0%
TTTGCTGAAGATGCAGCTGC+AGG + chr4.1:76875620-76875639 MS.gene073526:CDS 50.0%
! AAGGCTTGGTACAAGGTCCA+AGG + chr4.1:76876402-76876421 MS.gene073526:CDS 50.0%
!! ATCACCTTCGTCGGCTTCTA+AGG + chr4.1:76875976-76875995 MS.gene073526:CDS 50.0%
ATGCAGCTGCAGGGTCACTT+GGG + chr4.1:76875630-76875649 MS.gene073526:CDS 55.0%
GATGCAGCTGCAGGGTCACT+TGG + chr4.1:76875629-76875648 MS.gene073526:CDS 60.0%
TGCAGCTGCAGGGTCACTTG+GGG + chr4.1:76875631-76875650 MS.gene073526:CDS 60.0%
GCAGCTGCAGGGTCACTTGG+GGG + chr4.1:76875632-76875651 MS.gene073526:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 76875497 76876429 76875497 ID=MS.gene073526
chr4.1 mRNA 76875497 76876429 76875497 ID=MS.gene073526.t1;Parent=MS.gene073526
chr4.1 exon 76875497 76876429 76875497 ID=MS.gene073526.t1.exon1;Parent=MS.gene073526.t1
chr4.1 CDS 76875497 76876429 76875497 ID=cds.MS.gene073526.t1;Parent=MS.gene073526.t1
Gene Sequence

>MS.gene073526

ATGGAACAGGATCAATGTTGGGTTCAAACAAAGAGAAAGTACTCAATGAGTAATTCAAATCTTGCTTGTTCACTAACTAACCCTTCTTCTTCTTGTTATGGTGATTCATGGGAAGAACAAGCTTTTGCTGAAGATGCAGCTGCAGGGTCACTTGGGGGATGTATATGGCCACCAAGATCATATTCTTGTAGCTTTTGTAGAAGAGAGTTTAGGTCAGCACAAGCATTAGGTGGACATATGAATGTTCATAGAAAAGATAGAGCAAGACTCAAACAACAACCACAAAATGATCAGATTCTTTATCATAATCATCATCATCACCATCATCACGAGCTTGAAATAGCAACTCATCATGTTCAAAAGCCACTTATTCAAGATGTTTATAATAGTAACCTATTTCCTAATTCTCCTACTTGTGGTTTGGTTTATAATAAAACTAACCCTAATTCTCATCTTGATCATTTCGCATCTTCAACTTCATCACCTTCGTCGGCTTCTAAGGCTTTATTCGGAGATGAAACCCTAATTTCTCCTTTCAACTCTTCTGAATCATGGCTGAATTTACCTGGAAATGATATATTATGTTCAACAAAGTTTCAACTTGCTGTTGATAATTTTGATCATAAAGTCTCCAAAGGTATAATAGATTCTAGGTTTCGAGGTAAAGGGGGAAACGACAACGAAAGTGATGTAGCTATGAGCTTGAATTTGGTTTTGTGTAGAACTCATCCACTTGTTGAATTTGAGAGTACTAAAGAAGAAGATTTCAATTGCAAGAAGAGGAAAAAAGATTCTTCATCAAATCAATTATTCTCTAACTCAAGCACCTTTGATAAACAACAAAATATGCAAGCAAAGATGTTTGAGTTCAACCCTAATTCAATTGAGGAGGTAGATCTGGAGTTAAGGCTTGGTACAAGGTCCAAGGTATAG

Protein sequence

>MS.gene073526.t1

MEQDQCWVQTKRKYSMSNSNLACSLTNPSSSCYGDSWEEQAFAEDAAAGSLGGCIWPPRSYSCSFCRREFRSAQALGGHMNVHRKDRARLKQQPQNDQILYHNHHHHHHHELEIATHHVQKPLIQDVYNSNLFPNSPTCGLVYNKTNPNSHLDHFASSTSSPSSASKALFGDETLISPFNSSESWLNLPGNDILCSTKFQLAVDNFDHKVSKGIIDSRFRGKGGNDNESDVAMSLNLVLCRTHPLVEFESTKEEDFNCKKRKKDSSSNQLFSNSSTFDKQQNMQAKMFEFNPNSIEEVDLELRLGTRSKV