Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44712.t1 | AFK38431.1 | 96 | 151 | 6 | 0 | 1 | 151 | 1 | 151 | 4.40E-81 | 310.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44712.t1 | Q9FLV0 | 35.4 | 147 | 93 | 2 | 5 | 151 | 5 | 149 | 1.5e-21 | 104.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44712.t1 | A0A396GTP8 | 96.0 | 151 | 6 | 0 | 1 | 151 | 1 | 151 | 3.2e-81 | 310.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene44712 | -0.816975 | 4.19E-52 | -1.69E-46 |
| MS.gene049171 | MS.gene44712 | -0.805002 | 1.65E-49 | -1.69E-46 |
| MS.gene049222 | MS.gene44712 | -0.800796 | 1.22E-48 | -1.69E-46 |
| MS.gene049265 | MS.gene44712 | 0.812298 | 4.55E-51 | -1.69E-46 |
| MS.gene04932 | MS.gene44712 | 0.818875 | 1.56E-52 | -1.69E-46 |
| MS.gene049549 | MS.gene44712 | 0.859599 | 3.70E-63 | -1.69E-46 |
| MS.gene049611 | MS.gene44712 | 0.811335 | 7.37E-51 | -1.69E-46 |
| MS.gene049634 | MS.gene44712 | 0.821678 | 3.55E-53 | -1.69E-46 |
| MS.gene050095 | MS.gene44712 | 0.813271 | 2.79E-51 | -1.69E-46 |
| MS.gene050096 | MS.gene44712 | 0.827216 | 1.77E-54 | -1.69E-46 |
| MS.gene050380 | MS.gene44712 | 0.819607 | 1.06E-52 | -1.69E-46 |
| MS.gene050381 | MS.gene44712 | 0.821436 | 4.04E-53 | -1.69E-46 |
| MS.gene050504 | MS.gene44712 | -0.800677 | 1.29E-48 | -1.69E-46 |
| MS.gene050593 | MS.gene44712 | -0.804147 | 2.48E-49 | -1.69E-46 |
| MS.gene050647 | MS.gene44712 | 0.840531 | 8.22E-58 | -1.69E-46 |
| MS.gene050720 | MS.gene44712 | 0.82925 | 5.72E-55 | -1.69E-46 |
| MS.gene050809 | MS.gene44712 | 0.806937 | 6.45E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene44712 | -0.803397 | 3.56E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene44712 | -0.803978 | 2.69E-49 | -1.69E-46 |
| MS.gene05104 | MS.gene44712 | 0.820046 | 8.44E-53 | -1.69E-46 |
| MS.gene051127 | MS.gene44712 | 0.813551 | 2.42E-51 | -1.69E-46 |
| MS.gene051131 | MS.gene44712 | 0.846644 | 1.91E-59 | -1.69E-46 |
| MS.gene051138 | MS.gene44712 | 0.814773 | 1.30E-51 | -1.69E-46 |
| MS.gene051158 | MS.gene44712 | 0.832518 | 9.03E-56 | -1.69E-46 |
| MS.gene051632 | MS.gene44712 | 0.811079 | 8.37E-51 | -1.69E-46 |
| MS.gene051844 | MS.gene44712 | 0.814015 | 1.91E-51 | -1.69E-46 |
| MS.gene051874 | MS.gene44712 | -0.808603 | 2.86E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene44712 | 0.810029 | 1.41E-50 | -1.69E-46 |
| MS.gene051996 | MS.gene44712 | 0.813096 | 3.04E-51 | -1.69E-46 |
| MS.gene052211 | MS.gene44712 | 0.86665 | 2.44E-65 | -1.69E-46 |
| MS.gene052212 | MS.gene44712 | 0.840806 | 6.96E-58 | -1.69E-46 |
| MS.gene05240 | MS.gene44712 | 0.856985 | 2.22E-62 | -1.69E-46 |
| MS.gene05244 | MS.gene44712 | 0.826971 | 2.02E-54 | -1.69E-46 |
| MS.gene052606 | MS.gene44712 | 0.828875 | 7.05E-55 | -1.69E-46 |
| MS.gene052805 | MS.gene44712 | 0.811241 | 7.72E-51 | -1.69E-46 |
| MS.gene053031 | MS.gene44712 | 0.805291 | 1.43E-49 | -1.69E-46 |
| MS.gene053033 | MS.gene44712 | 0.82114 | 4.73E-53 | -1.69E-46 |
| MS.gene053403 | MS.gene44712 | 0.813764 | 2.17E-51 | -1.69E-46 |
| MS.gene053406 | MS.gene44712 | 0.878801 | 2.09E-69 | -1.69E-46 |
| MS.gene053420 | MS.gene44712 | 0.838413 | 2.92E-57 | -1.69E-46 |
| MS.gene053488 | MS.gene44712 | 0.800514 | 1.39E-48 | -1.69E-46 |
| MS.gene053574 | MS.gene44712 | -0.802038 | 6.78E-49 | -1.69E-46 |
| MS.gene054138 | MS.gene44712 | 0.815679 | 8.17E-52 | -1.69E-46 |
| MS.gene054146 | MS.gene44712 | 0.874161 | 8.38E-68 | -1.69E-46 |
| MS.gene054147 | MS.gene44712 | 0.831081 | 2.04E-55 | -1.69E-46 |
| MS.gene054181 | MS.gene44712 | 0.813373 | 2.64E-51 | -1.69E-46 |
| MS.gene054277 | MS.gene44712 | 0.808786 | 2.61E-50 | -1.69E-46 |
| MS.gene054290 | MS.gene44712 | 0.830048 | 3.66E-55 | -1.69E-46 |
| MS.gene054421 | MS.gene44712 | 0.819363 | 1.21E-52 | -1.69E-46 |
| MS.gene054595 | MS.gene44712 | -0.805319 | 1.41E-49 | -1.69E-46 |
| MS.gene054706 | MS.gene44712 | 0.837411 | 5.28E-57 | -1.69E-46 |
| MS.gene054742 | MS.gene44712 | 0.851183 | 1.05E-60 | -1.69E-46 |
| MS.gene054884 | MS.gene44712 | 0.802956 | 4.39E-49 | -1.69E-46 |
| MS.gene055124 | MS.gene44712 | 0.821132 | 4.75E-53 | -1.69E-46 |
| MS.gene055154 | MS.gene44712 | 0.825793 | 3.86E-54 | -1.69E-46 |
| MS.gene055158 | MS.gene44712 | 0.802911 | 4.48E-49 | -1.69E-46 |
| MS.gene055159 | MS.gene44712 | 0.817381 | 3.40E-52 | -1.69E-46 |
| MS.gene055202 | MS.gene44712 | 0.817287 | 3.57E-52 | -1.69E-46 |
| MS.gene055240 | MS.gene44712 | 0.845658 | 3.54E-59 | -1.69E-46 |
| MS.gene055502 | MS.gene44712 | -0.814552 | 1.45E-51 | -1.69E-46 |
| MS.gene055577 | MS.gene44712 | -0.812632 | 3.85E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene44712 | -0.805941 | 1.05E-49 | -1.69E-46 |
| MS.gene055959 | MS.gene44712 | 0.813859 | 2.07E-51 | -1.69E-46 |
| MS.gene056076 | MS.gene44712 | 0.810163 | 1.32E-50 | -1.69E-46 |
| MS.gene056218 | MS.gene44712 | 0.840885 | 6.64E-58 | -1.69E-46 |
| MS.gene056224 | MS.gene44712 | 0.830111 | 3.53E-55 | -1.69E-46 |
| MS.gene056330 | MS.gene44712 | 0.831016 | 2.12E-55 | -1.69E-46 |
| MS.gene056504 | MS.gene44712 | -0.803571 | 3.27E-49 | -1.69E-46 |
| MS.gene056590 | MS.gene44712 | 0.828809 | 7.32E-55 | -1.69E-46 |
| MS.gene056591 | MS.gene44712 | 0.827934 | 1.19E-54 | -1.69E-46 |
| MS.gene056680 | MS.gene44712 | 0.82223 | 2.65E-53 | -1.69E-46 |
| MS.gene056748 | MS.gene44712 | 0.822275 | 2.58E-53 | -1.69E-46 |
| MS.gene056856 | MS.gene44712 | 0.810465 | 1.14E-50 | -1.69E-46 |
| MS.gene056920 | MS.gene44712 | 0.856651 | 2.79E-62 | -1.69E-46 |
| MS.gene056922 | MS.gene44712 | 0.808184 | 3.51E-50 | -1.69E-46 |
| MS.gene056923 | MS.gene44712 | 0.80713 | 5.87E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene44712 | 0.804043 | 2.61E-49 | -1.69E-46 |
| MS.gene057086 | MS.gene44712 | -0.802229 | 6.19E-49 | -1.69E-46 |
| MS.gene057261 | MS.gene44712 | 0.836774 | 7.68E-57 | -1.69E-46 |
| MS.gene057544 | MS.gene44712 | 0.846875 | 1.65E-59 | -1.69E-46 |
| MS.gene057547 | MS.gene44712 | 0.80131 | 9.56E-49 | -1.69E-46 |
| MS.gene057550 | MS.gene44712 | 0.815731 | 7.95E-52 | -1.69E-46 |
| MS.gene057768 | MS.gene44712 | 0.804364 | 2.24E-49 | -1.69E-46 |
| MS.gene057770 | MS.gene44712 | 0.809856 | 1.54E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene44712 | 0.801268 | 9.76E-49 | -1.69E-46 |
| MS.gene058093 | MS.gene44712 | 0.808716 | 2.70E-50 | -1.69E-46 |
| MS.gene058099 | MS.gene44712 | 0.812479 | 4.15E-51 | -1.69E-46 |
| MS.gene058158 | MS.gene44712 | 0.813382 | 2.63E-51 | -1.69E-46 |
| MS.gene058171 | MS.gene44712 | 0.80283 | 4.66E-49 | -1.69E-46 |
| MS.gene058175 | MS.gene44712 | 0.801249 | 9.85E-49 | -1.69E-46 |
| MS.gene058218 | MS.gene44712 | 0.809947 | 1.47E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene44712 | -0.831279 | 1.83E-55 | -1.69E-46 |
| MS.gene058544 | MS.gene44712 | -0.805302 | 1.43E-49 | -1.69E-46 |
| MS.gene058603 | MS.gene44712 | 0.849741 | 2.66E-60 | -1.69E-46 |
| MS.gene058838 | MS.gene44712 | -0.81203 | 5.20E-51 | -1.69E-46 |
| MS.gene058840 | MS.gene44712 | 0.806674 | 7.33E-50 | -1.69E-46 |
| MS.gene059023 | MS.gene44712 | -0.803291 | 3.74E-49 | -1.69E-46 |
| MS.gene059037 | MS.gene44712 | 0.824515 | 7.74E-54 | -1.69E-46 |
| MS.gene059038 | MS.gene44712 | 0.805267 | 1.45E-49 | -1.69E-46 |
| MS.gene059328 | MS.gene44712 | 0.807547 | 4.79E-50 | -1.69E-46 |
| MS.gene059402 | MS.gene44712 | 0.812559 | 3.99E-51 | -1.69E-46 |
| MS.gene059892 | MS.gene44712 | 0.811647 | 6.31E-51 | -1.69E-46 |
| MS.gene059944 | MS.gene44712 | 0.800159 | 1.64E-48 | -1.69E-46 |
| MS.gene059953 | MS.gene44712 | 0.84034 | 9.22E-58 | -1.69E-46 |
| MS.gene059955 | MS.gene44712 | 0.890373 | 1.04E-73 | -1.69E-46 |
| MS.gene060087 | MS.gene44712 | 0.848756 | 5.00E-60 | -1.69E-46 |
| MS.gene060593 | MS.gene44712 | 0.829219 | 5.82E-55 | -1.69E-46 |
| MS.gene060835 | MS.gene44712 | -0.800252 | 1.57E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44712.t1 | MTR_6g015950 | 67.105 | 152 | 49 | 1 | 1 | 151 | 1 | 152 | 2.05e-72 | 220 |
| MS.gene44712.t1 | MTR_7g016090 | 63.576 | 151 | 55 | 0 | 1 | 151 | 1 | 151 | 1.87e-70 | 215 |
| MS.gene44712.t1 | MTR_7g016090 | 63.576 | 151 | 55 | 0 | 1 | 151 | 3 | 153 | 1.98e-70 | 215 |
| MS.gene44712.t1 | MTR_7g016080 | 61.438 | 153 | 57 | 1 | 1 | 151 | 1 | 153 | 6.24e-66 | 204 |
| MS.gene44712.t1 | MTR_1g070135 | 42.208 | 154 | 85 | 2 | 1 | 151 | 1 | 153 | 9.40e-38 | 130 |
| MS.gene44712.t1 | MTR_1g070135 | 42.208 | 154 | 85 | 2 | 1 | 151 | 1 | 153 | 2.03e-37 | 130 |
| MS.gene44712.t1 | MTR_1g070120 | 38.961 | 154 | 90 | 2 | 1 | 151 | 1 | 153 | 1.37e-35 | 126 |
| MS.gene44712.t1 | MTR_1g070080 | 38.562 | 153 | 85 | 4 | 5 | 151 | 4 | 153 | 2.07e-28 | 107 |
| MS.gene44712.t1 | MTR_1g070085 | 38.571 | 140 | 83 | 1 | 15 | 151 | 13 | 152 | 4.57e-28 | 106 |
| MS.gene44712.t1 | MTR_1g070085 | 38.571 | 140 | 83 | 1 | 15 | 151 | 13 | 152 | 4.57e-28 | 106 |
| MS.gene44712.t1 | MTR_3g092890 | 37.086 | 151 | 93 | 2 | 1 | 151 | 1 | 149 | 1.73e-27 | 104 |
| MS.gene44712.t1 | MTR_8g024120 | 35.762 | 151 | 88 | 4 | 5 | 151 | 11 | 156 | 7.11e-23 | 92.4 |
| MS.gene44712.t1 | MTR_7g081710 | 33.566 | 143 | 89 | 3 | 14 | 151 | 23 | 164 | 1.83e-21 | 88.6 |
| MS.gene44712.t1 | MTR_1g062110 | 34.839 | 155 | 89 | 4 | 5 | 151 | 30 | 180 | 3.26e-20 | 85.1 |
| MS.gene44712.t1 | MTR_1g062110 | 34.839 | 155 | 89 | 4 | 5 | 151 | 30 | 180 | 4.55e-20 | 85.1 |
| MS.gene44712.t1 | MTR_3g072820 | 31.206 | 141 | 95 | 2 | 13 | 151 | 15 | 155 | 2.13e-17 | 77.4 |
| MS.gene44712.t1 | MTR_5g059130 | 33.103 | 145 | 85 | 5 | 14 | 151 | 18 | 157 | 4.78e-17 | 76.6 |
| MS.gene44712.t1 | MTR_1g048940 | 30.769 | 143 | 91 | 3 | 14 | 151 | 22 | 161 | 6.44e-17 | 76.3 |
| MS.gene44712.t1 | MTR_7g102820 | 31.333 | 150 | 92 | 4 | 11 | 151 | 34 | 181 | 1.14e-15 | 72.8 |
| MS.gene44712.t1 | MTR_5g059140 | 29.371 | 143 | 97 | 3 | 13 | 151 | 15 | 157 | 8.71e-15 | 70.1 |
| MS.gene44712.t1 | MTR_3g070860 | 32.414 | 145 | 88 | 5 | 15 | 151 | 25 | 167 | 9.66e-15 | 70.1 |
| MS.gene44712.t1 | MTR_5g082750 | 31.507 | 146 | 88 | 5 | 15 | 151 | 24 | 166 | 3.24e-13 | 65.9 |
| MS.gene44712.t1 | MTR_3g072810 | 24.818 | 137 | 101 | 2 | 16 | 151 | 17 | 152 | 3.28e-13 | 65.9 |
| MS.gene44712.t1 | MTR_7g068650 | 34.000 | 150 | 85 | 7 | 14 | 151 | 24 | 171 | 3.89e-13 | 65.9 |
| MS.gene44712.t1 | MTR_5g032880 | 29.286 | 140 | 89 | 3 | 17 | 146 | 350 | 489 | 6.65e-13 | 65.5 |
| MS.gene44712.t1 | MTR_5g032880 | 28.889 | 135 | 89 | 4 | 20 | 151 | 19 | 149 | 3.04e-11 | 60.5 |
| MS.gene44712.t1 | MTR_7g029655 | 31.538 | 130 | 79 | 4 | 14 | 135 | 37 | 164 | 7.84e-13 | 65.1 |
| MS.gene44712.t1 | MTR_7g062550 | 38.596 | 114 | 61 | 4 | 39 | 147 | 60 | 169 | 1.44e-12 | 64.3 |
| MS.gene44712.t1 | MTR_4g015790 | 32.075 | 159 | 85 | 6 | 14 | 151 | 23 | 179 | 2.83e-12 | 63.2 |
| MS.gene44712.t1 | MTR_1g070185 | 33.333 | 120 | 75 | 3 | 14 | 129 | 43 | 161 | 3.18e-12 | 63.2 |
| MS.gene44712.t1 | MTR_7g062570 | 35.593 | 118 | 67 | 4 | 39 | 151 | 55 | 168 | 6.22e-12 | 62.4 |
| MS.gene44712.t1 | MTR_3g043900 | 29.104 | 134 | 89 | 3 | 15 | 144 | 28 | 159 | 1.05e-11 | 61.6 |
| MS.gene44712.t1 | MTR_5g032870 | 27.857 | 140 | 94 | 3 | 19 | 151 | 18 | 157 | 1.21e-11 | 61.6 |
| MS.gene44712.t1 | MTR_3g045230 | 27.273 | 132 | 91 | 2 | 17 | 144 | 30 | 160 | 1.48e-11 | 61.2 |
| MS.gene44712.t1 | MTR_5g011250 | 25.503 | 149 | 99 | 3 | 14 | 150 | 17 | 165 | 2.85e-11 | 60.5 |
| MS.gene44712.t1 | MTR_4g099380 | 30.612 | 98 | 67 | 1 | 39 | 136 | 174 | 270 | 8.24e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44712.t1 | AT5G24530 | 35.099 | 151 | 96 | 2 | 1 | 151 | 1 | 149 | 4.08e-26 | 101 |
| MS.gene44712.t1 | AT4G10500 | 36.129 | 155 | 93 | 4 | 1 | 151 | 6 | 158 | 3.51e-22 | 90.9 |
| MS.gene44712.t1 | AT4G10490 | 36.184 | 152 | 92 | 3 | 4 | 151 | 6 | 156 | 9.77e-22 | 89.4 |
| MS.gene44712.t1 | AT5G07480 | 34.752 | 141 | 83 | 5 | 16 | 151 | 24 | 160 | 1.04e-19 | 84.0 |
| MS.gene44712.t1 | AT5G08640 | 32.432 | 148 | 98 | 2 | 6 | 151 | 9 | 156 | 2.81e-18 | 80.1 |
| MS.gene44712.t1 | AT5G08640 | 32.432 | 148 | 98 | 2 | 6 | 151 | 9 | 156 | 2.81e-18 | 80.1 |
| MS.gene44712.t1 | AT2G44800 | 32.215 | 149 | 80 | 4 | 16 | 151 | 27 | 167 | 6.22e-18 | 79.3 |
| MS.gene44712.t1 | AT5G63590 | 39.167 | 120 | 68 | 3 | 34 | 150 | 9 | 126 | 1.06e-15 | 72.8 |
| MS.gene44712.t1 | AT3G60290 | 34.266 | 143 | 85 | 5 | 16 | 151 | 27 | 167 | 1.78e-15 | 72.4 |
| MS.gene44712.t1 | AT5G63580 | 32.231 | 121 | 75 | 3 | 34 | 151 | 14 | 130 | 2.12e-15 | 70.9 |
| MS.gene44712.t1 | AT5G63580 | 32.231 | 121 | 75 | 3 | 34 | 151 | 14 | 130 | 2.17e-15 | 71.2 |
| MS.gene44712.t1 | AT5G63600 | 35.338 | 133 | 75 | 5 | 23 | 151 | 15 | 140 | 5.77e-14 | 67.8 |
| MS.gene44712.t1 | AT5G63600 | 35.338 | 133 | 75 | 5 | 23 | 151 | 15 | 140 | 6.53e-14 | 67.8 |
| MS.gene44712.t1 | AT2G36690 | 30.201 | 149 | 90 | 4 | 14 | 151 | 30 | 175 | 2.44e-13 | 66.2 |
| MS.gene44712.t1 | AT2G36690 | 30.201 | 149 | 90 | 4 | 14 | 151 | 30 | 175 | 2.44e-13 | 66.2 |
| MS.gene44712.t1 | AT5G43935 | 33.913 | 115 | 72 | 2 | 37 | 150 | 17 | 128 | 9.93e-13 | 64.3 |
| MS.gene44712.t1 | AT3G11180 | 31.788 | 151 | 83 | 6 | 14 | 151 | 65 | 208 | 3.91e-12 | 63.2 |
| MS.gene44712.t1 | AT2G38240 | 35.461 | 141 | 85 | 5 | 15 | 151 | 22 | 160 | 4.20e-12 | 62.8 |
| MS.gene44712.t1 | AT3G11180 | 31.788 | 151 | 83 | 6 | 14 | 151 | 65 | 208 | 5.37e-12 | 62.8 |
| MS.gene44712.t1 | AT3G12900 | 31.429 | 140 | 90 | 4 | 14 | 149 | 31 | 168 | 6.02e-12 | 62.4 |
| MS.gene44712.t1 | AT1G06640 | 32.576 | 132 | 64 | 5 | 10 | 129 | 41 | 159 | 1.29e-11 | 61.2 |
| MS.gene44712.t1 | AT1G06640 | 33.333 | 126 | 71 | 4 | 10 | 129 | 41 | 159 | 1.41e-11 | 60.8 |
| MS.gene44712.t1 | AT1G06640 | 32.576 | 132 | 64 | 5 | 10 | 129 | 41 | 159 | 2.11e-11 | 60.8 |
| MS.gene44712.t1 | AT3G13610 | 31.061 | 132 | 88 | 3 | 15 | 144 | 37 | 167 | 4.57e-11 | 60.1 |
| MS.gene44712.t1 | AT1G55290 | 31.579 | 133 | 87 | 3 | 15 | 144 | 36 | 167 | 6.47e-11 | 59.3 |
| MS.gene44712.t1 | AT5G63595 | 34.167 | 120 | 68 | 3 | 35 | 151 | 21 | 132 | 6.57e-11 | 58.9 |
Find 38 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTATTGGAGGGACACATTA+AGG | 0.317719 | 7.2:+81508828 | MS.gene44712:CDS |
| GCCGTGCCGGGTCTACTTTC+CGG | 0.323728 | 7.2:-81508016 | None:intergenic |
| CAGGTGAATTCATGGAGTTT+TGG | 0.325921 | 7.2:+81508867 | MS.gene44712:CDS |
| TTGAGAGCTTCTGAGGAGTA+TGG | 0.335017 | 7.2:+81508115 | MS.gene44712:CDS |
| TCCACCTTCAGGTGAATTCA+TGG | 0.337468 | 7.2:+81508859 | MS.gene44712:CDS |
| GGCACGGCGTCTGTGATTTC+CGG | 0.339540 | 7.2:+81508031 | MS.gene44712:CDS |
| AGGCATATTTGTCCACCTTC+AGG | 0.341469 | 7.2:+81508848 | MS.gene44712:CDS |
| TAGAGTCTACAACTTCCATT+AGG | 0.351569 | 7.2:-81508770 | None:intergenic |
| CACCACCGGGATTATCTTGC+CGG | 0.356208 | 7.2:-81508050 | None:intergenic |
| TGGAGTTTCCAAGGAGTTGA+TGG | 0.384054 | 7.2:+81508673 | MS.gene44712:CDS |
| TCGATTTACCGGTATCTTGT+AGG | 0.387301 | 7.2:-81508899 | None:intergenic |
| GGTGGTTGCACATACGATAA+AGG | 0.428892 | 7.2:-81507995 | None:intergenic |
| ATAATCCCGGTGGTGGATCT+TGG | 0.440681 | 7.2:+81508058 | MS.gene44712:CDS |
| GAAATCACAGACGCCGTGCC+GGG | 0.455321 | 7.2:-81508028 | None:intergenic |
| AAAAGATAAGTGAAAGTTCA+AGG | 0.463084 | 7.2:+81508744 | MS.gene44712:CDS |
| GAAAGTTCAAGGGACCCTAA+TGG | 0.475802 | 7.2:+81508755 | MS.gene44712:CDS |
| TGTATCATCCATCAACTCCT+TGG | 0.485838 | 7.2:-81508681 | None:intergenic |
| AGAGTCTACAACTTCCATTA+GGG | 0.490244 | 7.2:-81508769 | None:intergenic |
| ATAACATGCTTGTCTCTAGC+TGG | 0.495915 | 7.2:+81507951 | MS.gene44712:CDS |
| GGAAATCACAGACGCCGTGC+CGG | 0.506940 | 7.2:-81508029 | None:intergenic |
| CAACCACCGGAAAGTAGACC+CGG | 0.523348 | 7.2:+81508010 | MS.gene44712:CDS |
| ATCCCGGTGGTGGATCTTGG+TGG | 0.536047 | 7.2:+81508061 | MS.gene44712:CDS |
| TGTTAACCATGGAGTTTCCA+AGG | 0.540398 | 7.2:+81508664 | MS.gene44712:CDS |
| CGGCAAGATAATCCCGGTGG+TGG | 0.552157 | 7.2:+81508051 | MS.gene44712:CDS |
| CACAAAAGCCTACAAGATAC+CGG | 0.561375 | 7.2:+81508891 | MS.gene44712:CDS |
| ATTTATAAGGTTGTTAACCA+TGG | 0.564655 | 7.2:+81508653 | MS.gene44712:intron |
| AAAGATAAGTGAAAGTTCAA+GGG | 0.568605 | 7.2:+81508745 | MS.gene44712:CDS |
| ACCGGAAAGTAGACCCGGCA+CGG | 0.589489 | 7.2:+81508015 | MS.gene44712:CDS |
| GACCACCAAGATCCACCACC+GGG | 0.598905 | 7.2:-81508063 | None:intergenic |
| GATTTCCGGCAAGATAATCC+CGG | 0.610043 | 7.2:+81508045 | MS.gene44712:CDS |
| TCAACTCCTTGGAAACTCCA+TGG | 0.618739 | 7.2:-81508670 | None:intergenic |
| TGACCACCAAGATCCACCAC+CGG | 0.619625 | 7.2:-81508064 | None:intergenic |
| AACTCCATGAATTCACCTGA+AGG | 0.620817 | 7.2:-81508863 | None:intergenic |
| TCCATGAATTCACCTGAAGG+TGG | 0.625343 | 7.2:-81508860 | None:intergenic |
| TTTATCGTATGTGCAACCAC+CGG | 0.630904 | 7.2:+81507997 | MS.gene44712:CDS |
| AAGTCGTGAGATTAATAACA+AGG | 0.641193 | 7.2:+81508796 | MS.gene44712:CDS |
| GTGCCGGGTCTACTTTCCGG+TGG | 0.645714 | 7.2:-81508013 | None:intergenic |
| TTCCGGCAAGATAATCCCGG+TGG | 0.727151 | 7.2:+81508048 | MS.gene44712:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATTAAGAAGAAAATTCTA+GGG | + | chr7.2:81508415-81508434 | MS.gene44712:intron | 15.0% |
| !! | TTAATTAAGAAGAAAATTCT+AGG | + | chr7.2:81508414-81508433 | MS.gene44712:intron | 15.0% |
| !!! | TTCAATGATTTATTTTCATT+AGG | + | chr7.2:81508528-81508547 | MS.gene44712:intron | 15.0% |
| !! | AACTTGTGGATTTATTTATA+AGG | + | chr7.2:81508640-81508659 | MS.gene44712:intron | 20.0% |
| !! | ACAAAAATTAAAAAATGTCC+AGG | + | chr7.2:81508261-81508280 | MS.gene44712:intron | 20.0% |
| !! | ATATCAAGATTTAAATCGTA+AGG | + | chr7.2:81508565-81508584 | MS.gene44712:intron | 20.0% |
| !! | ATTCTAATTATCACAGAAAA+AGG | - | chr7.2:81508471-81508490 | None:intergenic | 20.0% |
| !! | TAACGACAAACATATATATA+TGG | - | chr7.2:81508608-81508627 | None:intergenic | 20.0% |
| !! | TATTTAATGTGAAAAACTTG+TGG | + | chr7.2:81508626-81508645 | MS.gene44712:intron | 20.0% |
| !! | TCTAGTCACAATTATAATTA+TGG | - | chr7.2:81508337-81508356 | None:intergenic | 20.0% |
| !! | TTCTAATTATCACAGAAAAA+GGG | - | chr7.2:81508470-81508489 | None:intergenic | 20.0% |
| !! | TTTGAATATTTCACTCTATA+TGG | + | chr7.2:81508178-81508197 | MS.gene44712:intron | 20.0% |
| ! | AAAAGATAAGTGAAAGTTCA+AGG | + | chr7.2:81508744-81508763 | MS.gene44712:CDS | 25.0% |
| ! | AAAGATAAGTGAAAGTTCAA+GGG | + | chr7.2:81508745-81508764 | MS.gene44712:CDS | 25.0% |
| ! | AAATTAAAAAATGTCCAGGT+TGG | + | chr7.2:81508265-81508284 | MS.gene44712:intron | 25.0% |
| ! | ATTTATAAGGTTGTTAACCA+TGG | + | chr7.2:81508653-81508672 | MS.gene44712:intron | 25.0% |
| !! | TCACTTATCTTTTCTAATTC+AGG | - | chr7.2:81508737-81508756 | None:intergenic | 25.0% |
| !! | TGATACAATGAACATTTTCA+AGG | + | chr7.2:81508697-81508716 | MS.gene44712:CDS | 25.0% |
| !!! | TATTTTATTTTACACCAACC+TGG | - | chr7.2:81508282-81508301 | None:intergenic | 25.0% |
| AAGTCGTGAGATTAATAACA+AGG | + | chr7.2:81508796-81508815 | MS.gene44712:CDS | 30.0% | |
| CAAAATCTTAAGTACAAACC+TGG | - | chr7.2:81508147-81508166 | None:intergenic | 30.0% | |
| GAGTGAAATATTCAAACGTA+AGG | - | chr7.2:81508174-81508193 | None:intergenic | 30.0% | |
| TGATTGAACTTCAAATTTCC+AGG | + | chr7.2:81508443-81508462 | MS.gene44712:intron | 30.0% | |
| ! | ACAAGGATTTTGTTCAGTAT+TGG | + | chr7.2:81508813-81508832 | MS.gene44712:CDS | 30.0% |
| ! | GATAAGTGTGTTCTAAATTG+AGG | + | chr7.2:81508310-81508329 | MS.gene44712:intron | 30.0% |
| ! | TATCTTTTCTAATTCAGGCA+TGG | - | chr7.2:81508732-81508751 | None:intergenic | 30.0% |
| AGAGTCTACAACTTCCATTA+GGG | - | chr7.2:81508772-81508791 | None:intergenic | 35.0% | |
| TAGAGTCTACAACTTCCATT+AGG | - | chr7.2:81508773-81508792 | None:intergenic | 35.0% | |
| !! | AGGATTTTGTTCAGTATTGG+AGG | + | chr7.2:81508816-81508835 | MS.gene44712:CDS | 35.0% |
| !! | GGATTTTGTTCAGTATTGGA+GGG | + | chr7.2:81508817-81508836 | MS.gene44712:CDS | 35.0% |
| AACTCCATGAATTCACCTGA+AGG | - | chr7.2:81508866-81508885 | None:intergenic | 40.0% | |
| ATAACATGCTTGTCTCTAGC+TGG | + | chr7.2:81507951-81507970 | MS.gene44712:CDS | 40.0% | |
| CACAAAAGCCTACAAGATAC+CGG | + | chr7.2:81508891-81508910 | MS.gene44712:CDS | 40.0% | |
| TGTATCATCCATCAACTCCT+TGG | - | chr7.2:81508684-81508703 | None:intergenic | 40.0% | |
| TGTTAACCATGGAGTTTCCA+AGG | + | chr7.2:81508664-81508683 | MS.gene44712:CDS | 40.0% | |
| TTATCACAGAAAAAGGGTCC+TGG | - | chr7.2:81508464-81508483 | None:intergenic | 40.0% | |
| TTTATCGTATGTGCAACCAC+CGG | + | chr7.2:81507997-81508016 | MS.gene44712:CDS | 40.0% | |
| ! | AGTATTGGAGGGACACATTA+AGG | + | chr7.2:81508828-81508847 | MS.gene44712:CDS | 40.0% |
| ! | CAGGTGAATTCATGGAGTTT+TGG | + | chr7.2:81508867-81508886 | MS.gene44712:CDS | 40.0% |
| !! | TGAGGAGTATGGATTTTTCC+AGG | + | chr7.2:81508126-81508145 | MS.gene44712:CDS | 40.0% |
| AGGCATATTTGTCCACCTTC+AGG | + | chr7.2:81508848-81508867 | MS.gene44712:CDS | 45.0% | |
| GAAAGTTCAAGGGACCCTAA+TGG | + | chr7.2:81508755-81508774 | MS.gene44712:CDS | 45.0% | |
| GATTTCCGGCAAGATAATCC+CGG | + | chr7.2:81508045-81508064 | MS.gene44712:CDS | 45.0% | |
| GGTGGTTGCACATACGATAA+AGG | - | chr7.2:81507998-81508017 | None:intergenic | 45.0% | |
| TCAACTCCTTGGAAACTCCA+TGG | - | chr7.2:81508673-81508692 | None:intergenic | 45.0% | |
| TCCACCTTCAGGTGAATTCA+TGG | + | chr7.2:81508859-81508878 | MS.gene44712:CDS | 45.0% | |
| TCCATGAATTCACCTGAAGG+TGG | - | chr7.2:81508863-81508882 | None:intergenic | 45.0% | |
| TGGAGTTTCCAAGGAGTTGA+TGG | + | chr7.2:81508673-81508692 | MS.gene44712:CDS | 45.0% | |
| ! | CGGTATCTTGTAGGCTTTTG+TGG | - | chr7.2:81508893-81508912 | None:intergenic | 45.0% |
| ! | TTGAGAGCTTCTGAGGAGTA+TGG | + | chr7.2:81508115-81508134 | MS.gene44712:CDS | 45.0% |
| !!! | ATTTTTTAATTTTTGTATAT+AGG | - | chr7.2:81508257-81508276 | None:intergenic | 5.0% |
| !! | ATAATCCCGGTGGTGGATCT+TGG | + | chr7.2:81508058-81508077 | MS.gene44712:CDS | 50.0% |
| !!! | GCACGTTTTGAGAGCTTCTG+AGG | + | chr7.2:81508108-81508127 | MS.gene44712:CDS | 50.0% |
| CAACCACCGGAAAGTAGACC+CGG | + | chr7.2:81508010-81508029 | MS.gene44712:CDS | 55.0% | |
| CACCACCGGGATTATCTTGC+CGG | - | chr7.2:81508053-81508072 | None:intergenic | 55.0% | |
| TGACCACCAAGATCCACCAC+CGG | - | chr7.2:81508067-81508086 | None:intergenic | 55.0% | |
| TTCCGGCAAGATAATCCCGG+TGG | + | chr7.2:81508048-81508067 | MS.gene44712:CDS | 55.0% | |
| ACCGGAAAGTAGACCCGGCA+CGG | + | chr7.2:81508015-81508034 | MS.gene44712:CDS | 60.0% | |
| CGGCAAGATAATCCCGGTGG+TGG | + | chr7.2:81508051-81508070 | MS.gene44712:CDS | 60.0% | |
| GAAATCACAGACGCCGTGCC+GGG | - | chr7.2:81508031-81508050 | None:intergenic | 60.0% | |
| GACCACCAAGATCCACCACC+GGG | - | chr7.2:81508066-81508085 | None:intergenic | 60.0% | |
| GGAAATCACAGACGCCGTGC+CGG | - | chr7.2:81508032-81508051 | None:intergenic | 60.0% | |
| GGCACGGCGTCTGTGATTTC+CGG | + | chr7.2:81508031-81508050 | MS.gene44712:CDS | 60.0% | |
| !! | ATCCCGGTGGTGGATCTTGG+TGG | + | chr7.2:81508061-81508080 | MS.gene44712:CDS | 60.0% |
| GCCGTGCCGGGTCTACTTTC+CGG | - | chr7.2:81508019-81508038 | None:intergenic | 65.0% | |
| GTGCCGGGTCTACTTTCCGG+TGG | - | chr7.2:81508016-81508035 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 81507947 | 81508916 | 81507947 | ID=MS.gene44712 |
| chr7.2 | mRNA | 81507947 | 81508916 | 81507947 | ID=MS.gene44712.t1;Parent=MS.gene44712 |
| chr7.2 | exon | 81507947 | 81508147 | 81507947 | ID=MS.gene44712.t1.exon1;Parent=MS.gene44712.t1 |
| chr7.2 | CDS | 81507947 | 81508147 | 81507947 | ID=cds.MS.gene44712.t1;Parent=MS.gene44712.t1 |
| chr7.2 | exon | 81508662 | 81508916 | 81508662 | ID=MS.gene44712.t1.exon2;Parent=MS.gene44712.t1 |
| chr7.2 | CDS | 81508662 | 81508916 | 81508662 | ID=cds.MS.gene44712.t1;Parent=MS.gene44712.t1 |
| Gene Sequence |
| Protein sequence |
