Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80687.t1 | XP_003616622.1 | 97 | 99 | 3 | 0 | 64 | 162 | 31 | 129 | 3.70E-49 | 204.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80687.t1 | P59234 | 78.6 | 98 | 21 | 0 | 64 | 161 | 31 | 128 | 3.1e-41 | 169.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80687.t1 | G7KGZ8 | 97.0 | 99 | 3 | 0 | 64 | 162 | 31 | 129 | 2.6e-49 | 204.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene80687 | 0.856441 | 3.21E-62 | -1.69E-46 |
| MS.gene049159 | MS.gene80687 | 0.800003 | 1.77E-48 | -1.69E-46 |
| MS.gene04918 | MS.gene80687 | 0.821907 | 3.14E-53 | -1.69E-46 |
| MS.gene049254 | MS.gene80687 | 0.850103 | 2.11E-60 | -1.69E-46 |
| MS.gene049283 | MS.gene80687 | 0.803925 | 2.76E-49 | -1.69E-46 |
| MS.gene049343 | MS.gene80687 | 0.816085 | 6.63E-52 | -1.69E-46 |
| MS.gene049350 | MS.gene80687 | 0.831191 | 1.92E-55 | -1.69E-46 |
| MS.gene049368 | MS.gene80687 | 0.819529 | 1.11E-52 | -1.69E-46 |
| MS.gene049404 | MS.gene80687 | 0.81356 | 2.41E-51 | -1.69E-46 |
| MS.gene049499 | MS.gene80687 | 0.814453 | 1.53E-51 | -1.69E-46 |
| MS.gene049585 | MS.gene80687 | 0.805904 | 1.07E-49 | -1.69E-46 |
| MS.gene049711 | MS.gene80687 | 0.85238 | 4.80E-61 | -1.69E-46 |
| MS.gene049807 | MS.gene80687 | 0.84615 | 2.60E-59 | -1.69E-46 |
| MS.gene049850 | MS.gene80687 | 0.816302 | 5.93E-52 | -1.69E-46 |
| MS.gene049983 | MS.gene80687 | 0.836167 | 1.10E-56 | -1.69E-46 |
| MS.gene050273 | MS.gene80687 | 0.803873 | 2.83E-49 | -1.69E-46 |
| MS.gene050559 | MS.gene80687 | 0.842515 | 2.47E-58 | -1.69E-46 |
| MS.gene050561 | MS.gene80687 | 0.840093 | 1.07E-57 | -1.69E-46 |
| MS.gene050562 | MS.gene80687 | 0.834399 | 3.06E-56 | -1.69E-46 |
| MS.gene050646 | MS.gene80687 | 0.817373 | 3.41E-52 | -1.69E-46 |
| MS.gene050654 | MS.gene80687 | 0.800722 | 1.26E-48 | -1.69E-46 |
| MS.gene050689 | MS.gene80687 | 0.817017 | 4.10E-52 | -1.69E-46 |
| MS.gene051198 | MS.gene80687 | 0.803702 | 3.07E-49 | -1.69E-46 |
| MS.gene051201 | MS.gene80687 | 0.80736 | 5.25E-50 | -1.69E-46 |
| MS.gene051247 | MS.gene80687 | 0.817556 | 3.10E-52 | -1.69E-46 |
| MS.gene051279 | MS.gene80687 | 0.824298 | 8.71E-54 | -1.69E-46 |
| MS.gene051332 | MS.gene80687 | 0.824728 | 6.90E-54 | -1.69E-46 |
| MS.gene051343 | MS.gene80687 | 0.807132 | 5.87E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene80687 | 0.823741 | 1.18E-53 | -1.69E-46 |
| MS.gene051720 | MS.gene80687 | 0.823108 | 1.65E-53 | -1.69E-46 |
| MS.gene051744 | MS.gene80687 | 0.858864 | 6.15E-63 | -1.69E-46 |
| MS.gene051975 | MS.gene80687 | 0.819793 | 9.64E-53 | -1.69E-46 |
| MS.gene052037 | MS.gene80687 | 0.814818 | 1.27E-51 | -1.69E-46 |
| MS.gene052072 | MS.gene80687 | 0.800944 | 1.14E-48 | -1.69E-46 |
| MS.gene052307 | MS.gene80687 | 0.852224 | 5.31E-61 | -1.69E-46 |
| MS.gene052335 | MS.gene80687 | 0.816696 | 4.84E-52 | -1.69E-46 |
| MS.gene052459 | MS.gene80687 | 0.801914 | 7.19E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene80687 | 0.862629 | 4.43E-64 | -1.69E-46 |
| MS.gene052514 | MS.gene80687 | 0.803217 | 3.87E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene80687 | 0.858098 | 1.04E-62 | -1.69E-46 |
| MS.gene052518 | MS.gene80687 | 0.816926 | 4.30E-52 | -1.69E-46 |
| MS.gene052519 | MS.gene80687 | 0.847302 | 1.26E-59 | -1.69E-46 |
| MS.gene052533 | MS.gene80687 | 0.817313 | 3.52E-52 | -1.69E-46 |
| MS.gene052609 | MS.gene80687 | 0.830947 | 2.20E-55 | -1.69E-46 |
| MS.gene052773 | MS.gene80687 | 0.829301 | 5.56E-55 | -1.69E-46 |
| MS.gene052863 | MS.gene80687 | 0.822665 | 2.10E-53 | -1.69E-46 |
| MS.gene053006 | MS.gene80687 | 0.8036 | 3.23E-49 | -1.69E-46 |
| MS.gene053079 | MS.gene80687 | 0.84294 | 1.90E-58 | -1.69E-46 |
| MS.gene053144 | MS.gene80687 | 0.812652 | 3.81E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene80687 | 0.823947 | 1.05E-53 | -1.69E-46 |
| MS.gene053272 | MS.gene80687 | -0.851029 | 1.16E-60 | -1.69E-46 |
| MS.gene05350 | MS.gene80687 | 0.806209 | 9.19E-50 | -1.69E-46 |
| MS.gene053581 | MS.gene80687 | 0.800022 | 1.75E-48 | -1.69E-46 |
| MS.gene054142 | MS.gene80687 | 0.806273 | 8.91E-50 | -1.69E-46 |
| MS.gene054178 | MS.gene80687 | 0.818091 | 2.35E-52 | -1.69E-46 |
| MS.gene05423 | MS.gene80687 | 0.818106 | 2.33E-52 | -1.69E-46 |
| MS.gene054584 | MS.gene80687 | 0.801846 | 7.43E-49 | -1.69E-46 |
| MS.gene05458 | MS.gene80687 | 0.829563 | 4.80E-55 | -1.69E-46 |
| MS.gene054651 | MS.gene80687 | 0.87244 | 3.18E-67 | -1.69E-46 |
| MS.gene054775 | MS.gene80687 | 0.807486 | 4.94E-50 | -1.69E-46 |
| MS.gene055228 | MS.gene80687 | 0.801631 | 8.22E-49 | -1.69E-46 |
| MS.gene055313 | MS.gene80687 | 0.801024 | 1.09E-48 | -1.69E-46 |
| MS.gene055513 | MS.gene80687 | 0.860798 | 1.61E-63 | -1.69E-46 |
| MS.gene055724 | MS.gene80687 | 0.832142 | 1.12E-55 | -1.69E-46 |
| MS.gene055818 | MS.gene80687 | 0.838788 | 2.34E-57 | -1.69E-46 |
| MS.gene055841 | MS.gene80687 | 0.815709 | 8.04E-52 | -1.69E-46 |
| MS.gene055864 | MS.gene80687 | 0.810655 | 1.03E-50 | -1.69E-46 |
| MS.gene056050 | MS.gene80687 | 0.824314 | 8.63E-54 | -1.69E-46 |
| MS.gene056174 | MS.gene80687 | 0.820673 | 6.06E-53 | -1.69E-46 |
| MS.gene056308 | MS.gene80687 | 0.841701 | 4.05E-58 | -1.69E-46 |
| MS.gene056366 | MS.gene80687 | 0.817367 | 3.42E-52 | -1.69E-46 |
| MS.gene056507 | MS.gene80687 | 0.810071 | 1.38E-50 | -1.69E-46 |
| MS.gene056595 | MS.gene80687 | 0.812616 | 3.88E-51 | -1.69E-46 |
| MS.gene056677 | MS.gene80687 | 0.810297 | 1.24E-50 | -1.69E-46 |
| MS.gene056699 | MS.gene80687 | 0.885244 | 9.61E-72 | -1.69E-46 |
| MS.gene05686 | MS.gene80687 | 0.867884 | 9.84E-66 | -1.69E-46 |
| MS.gene05690 | MS.gene80687 | 0.818037 | 2.42E-52 | -1.69E-46 |
| MS.gene057034 | MS.gene80687 | 0.843567 | 1.29E-58 | -1.69E-46 |
| MS.gene057102 | MS.gene80687 | 0.873378 | 1.54E-67 | -1.69E-46 |
| MS.gene057132 | MS.gene80687 | 0.803452 | 3.46E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene80687 | 0.862883 | 3.70E-64 | -1.69E-46 |
| MS.gene057855 | MS.gene80687 | 0.80469 | 1.91E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene80687 | 0.826729 | 2.31E-54 | -1.69E-46 |
| MS.gene058008 | MS.gene80687 | 0.838187 | 3.34E-57 | -1.69E-46 |
| MS.gene058009 | MS.gene80687 | 0.839717 | 1.34E-57 | -1.69E-46 |
| MS.gene058010 | MS.gene80687 | 0.822999 | 1.75E-53 | -1.69E-46 |
| MS.gene058159 | MS.gene80687 | 0.800613 | 1.33E-48 | -1.69E-46 |
| MS.gene058355 | MS.gene80687 | 0.804411 | 2.19E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene80687 | 0.8394 | 1.62E-57 | -1.69E-46 |
| MS.gene058781 | MS.gene80687 | 0.822523 | 2.26E-53 | -1.69E-46 |
| MS.gene058783 | MS.gene80687 | 0.831137 | 1.98E-55 | -1.69E-46 |
| MS.gene058979 | MS.gene80687 | 0.823911 | 1.07E-53 | -1.69E-46 |
| MS.gene059146 | MS.gene80687 | 0.809182 | 2.15E-50 | -1.69E-46 |
| MS.gene059283 | MS.gene80687 | 0.804706 | 1.90E-49 | -1.69E-46 |
| MS.gene059570 | MS.gene80687 | 0.809548 | 1.79E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene80687 | 0.800955 | 1.13E-48 | -1.69E-46 |
| MS.gene059634 | MS.gene80687 | 0.808657 | 2.78E-50 | -1.69E-46 |
| MS.gene059774 | MS.gene80687 | 0.80974 | 1.63E-50 | -1.69E-46 |
| MS.gene059842 | MS.gene80687 | 0.815691 | 8.12E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene80687 | -0.844645 | 6.65E-59 | -1.69E-46 |
| MS.gene059965 | MS.gene80687 | 0.840008 | 1.13E-57 | -1.69E-46 |
| MS.gene060259 | MS.gene80687 | 0.801081 | 1.07E-48 | -1.69E-46 |
| MS.gene060525 | MS.gene80687 | 0.811127 | 8.18E-51 | -1.69E-46 |
| MS.gene060697 | MS.gene80687 | 0.805382 | 1.37E-49 | -1.69E-46 |
| MS.gene060777 | MS.gene80687 | 0.827841 | 1.25E-54 | -1.69E-46 |
| MS.gene060932 | MS.gene80687 | 0.824315 | 8.63E-54 | -1.69E-46 |
| MS.gene060935 | MS.gene80687 | 0.865322 | 6.42E-65 | -1.69E-46 |
| MS.gene060937 | MS.gene80687 | 0.86599 | 3.95E-65 | -1.69E-46 |
| MS.gene061100 | MS.gene80687 | 0.826627 | 2.45E-54 | -1.69E-46 |
| MS.gene061160 | MS.gene80687 | 0.80063 | 1.32E-48 | -1.69E-46 |
| MS.gene061188 | MS.gene80687 | 0.848516 | 5.83E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80687.t1 | MTR_5g082490 | 96.970 | 99 | 3 | 0 | 64 | 162 | 31 | 129 | 3.21e-67 | 201 |
| MS.gene80687.t1 | MTR_3g070330 | 77.551 | 98 | 22 | 0 | 64 | 161 | 54 | 151 | 1.21e-51 | 162 |
| MS.gene80687.t1 | MTR_3g070330 | 83.333 | 72 | 12 | 0 | 64 | 135 | 54 | 125 | 2.79e-39 | 130 |
| MS.gene80687.t1 | MTR_7g068250 | 71.084 | 83 | 24 | 0 | 64 | 146 | 31 | 113 | 2.55e-38 | 127 |
| MS.gene80687.t1 | MTR_2g078670 | 53.535 | 99 | 46 | 0 | 64 | 162 | 31 | 129 | 2.08e-30 | 107 |
| MS.gene80687.t1 | MTR_8g058785 | 62.667 | 75 | 28 | 0 | 64 | 138 | 31 | 105 | 1.43e-28 | 102 |
| MS.gene80687.t1 | MTR_8g061270 | 62.667 | 75 | 28 | 0 | 64 | 138 | 31 | 105 | 1.43e-28 | 102 |
| MS.gene80687.t1 | MTR_4g088505 | 58.667 | 75 | 31 | 0 | 64 | 138 | 31 | 105 | 7.24e-27 | 98.2 |
| MS.gene80687.t1 | MTR_8g027255 | 52.500 | 80 | 38 | 0 | 64 | 143 | 27 | 106 | 1.94e-26 | 96.7 |
| MS.gene80687.t1 | MTR_3g091840 | 54.667 | 75 | 34 | 0 | 64 | 138 | 27 | 101 | 3.08e-25 | 93.6 |
| MS.gene80687.t1 | MTR_1g077750 | 46.154 | 65 | 35 | 0 | 64 | 128 | 27 | 91 | 1.10e-18 | 76.6 |
| MS.gene80687.t1 | MTR_1g077760 | 51.852 | 54 | 26 | 0 | 81 | 134 | 1 | 54 | 2.80e-16 | 71.2 |
| MS.gene80687.t1 | MTR_4g088505 | 56.604 | 53 | 23 | 0 | 64 | 116 | 31 | 83 | 7.81e-16 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80687.t1 | AT2G40110 | 79.000 | 100 | 20 | 1 | 64 | 162 | 31 | 130 | 4.37e-52 | 162 |
| MS.gene80687.t1 | AT3G11230 | 69.697 | 99 | 30 | 0 | 64 | 162 | 31 | 129 | 9.40e-46 | 146 |
| MS.gene80687.t1 | AT3G11230 | 69.697 | 99 | 30 | 0 | 64 | 162 | 64 | 162 | 2.07e-45 | 146 |
| MS.gene80687.t1 | AT2G40110 | 80.822 | 73 | 14 | 0 | 64 | 136 | 31 | 103 | 1.42e-38 | 127 |
| MS.gene80687.t1 | AT3G55890 | 63.636 | 99 | 28 | 1 | 64 | 162 | 31 | 121 | 1.52e-38 | 127 |
| MS.gene80687.t1 | AT3G55890 | 63.636 | 99 | 28 | 1 | 64 | 162 | 31 | 121 | 1.52e-38 | 127 |
| MS.gene80687.t1 | AT3G08990 | 67.470 | 83 | 27 | 0 | 64 | 146 | 31 | 113 | 3.46e-36 | 122 |
| MS.gene80687.t1 | AT3G08990 | 67.470 | 83 | 27 | 0 | 64 | 146 | 31 | 113 | 3.46e-36 | 122 |
| MS.gene80687.t1 | AT3G08990 | 67.470 | 83 | 27 | 0 | 64 | 146 | 31 | 113 | 3.46e-36 | 122 |
| MS.gene80687.t1 | AT5G53940 | 56.566 | 99 | 43 | 0 | 64 | 162 | 31 | 129 | 2.49e-34 | 117 |
| MS.gene80687.t1 | AT3G11230 | 73.973 | 73 | 19 | 0 | 64 | 136 | 31 | 103 | 2.04e-33 | 114 |
| MS.gene80687.t1 | AT3G11230 | 75.000 | 72 | 18 | 0 | 64 | 135 | 64 | 135 | 4.52e-33 | 114 |
| MS.gene80687.t1 | AT3G55890 | 71.831 | 71 | 20 | 0 | 64 | 134 | 31 | 101 | 1.27e-32 | 112 |
| MS.gene80687.t1 | AT3G55890 | 71.831 | 71 | 20 | 0 | 64 | 134 | 31 | 101 | 1.27e-32 | 112 |
| MS.gene80687.t1 | AT4G27745 | 54.430 | 79 | 36 | 0 | 64 | 142 | 27 | 105 | 2.58e-26 | 96.3 |
| MS.gene80687.t1 | AT4G27745 | 54.430 | 79 | 36 | 0 | 64 | 142 | 27 | 105 | 2.58e-26 | 96.3 |
| MS.gene80687.t1 | AT4G27745 | 54.430 | 79 | 36 | 0 | 64 | 142 | 27 | 105 | 2.58e-26 | 96.3 |
| MS.gene80687.t1 | AT4G27745 | 54.430 | 79 | 36 | 0 | 64 | 142 | 27 | 105 | 2.58e-26 | 96.3 |
| MS.gene80687.t1 | AT2G40110 | 75.472 | 53 | 13 | 0 | 64 | 116 | 31 | 83 | 4.71e-24 | 90.1 |
| MS.gene80687.t1 | AT2G40110 | 75.472 | 53 | 13 | 0 | 64 | 116 | 31 | 83 | 4.71e-24 | 90.1 |
| MS.gene80687.t1 | AT4G27740 | 38.158 | 76 | 47 | 0 | 64 | 139 | 27 | 102 | 3.33e-14 | 65.1 |
Find 40 sgRNAs with CRISPR-Local
Find 113 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCCTGATGGCAGCAATTAT+TGG | 0.259271 | 5.4:-64976273 | MS.gene80687:CDS |
| TACAGTGTGAATGTTTCTAT+TGG | 0.322176 | 5.4:-64976799 | MS.gene80687:intron |
| TGGTGCTGTTTCGGCAGTTT+CGG | 0.322767 | 5.4:+64977972 | None:intergenic |
| TTGACCAGGTTCAAGGTGTC+TGG | 0.365136 | 5.4:-64976295 | MS.gene80687:intron |
| TCCTGATGGCAGCAATTATT+GGG | 0.368015 | 5.4:-64976272 | MS.gene80687:CDS |
| TGTTTCTATTGGAGAATTAG+AGG | 0.372709 | 5.4:-64976788 | MS.gene80687:CDS |
| CCGCTGCCATGGTGCTGTTT+CGG | 0.379880 | 5.4:+64977963 | None:intergenic |
| CAATGGTGCAAGATACAAAA+TGG | 0.397358 | 5.4:-64978044 | MS.gene80687:CDS |
| GTCATCAACCATTTGTGTAT+TGG | 0.406182 | 5.4:-64977941 | MS.gene80687:intron |
| TTTGTGTACCAATACACAAA+TGG | 0.440882 | 5.4:+64977933 | None:intergenic |
| ACCCAATAATTGCTGCCATC+AGG | 0.450327 | 5.4:+64976271 | None:intergenic |
| CAAGGATAACTTACACCTTA+CGG | 0.472783 | 5.4:+64977078 | None:intergenic |
| GAGGACAGAAATATGATGAC+TGG | 0.475545 | 5.4:-64976769 | MS.gene80687:CDS |
| GCAAATAGTCCTTCAAAAGT+AGG | 0.489411 | 5.4:+64977581 | None:intergenic |
| TTCAAGGTGTCTGGTCCTGA+TGG | 0.507863 | 5.4:-64976286 | MS.gene80687:CDS |
| GAGAATAGTTGACGCGGTAG+TGG | 0.515368 | 5.4:-64977997 | MS.gene80687:CDS |
| CAGGCTTTCCACAGCAGACA+TGG | 0.524156 | 5.4:-64977116 | MS.gene80687:CDS |
| CTACCGCGTCAACTATTCTC+AGG | 0.531580 | 5.4:+64978000 | None:intergenic |
| TTTCAACACCATGTGTTGCA+TGG | 0.544712 | 5.4:+64976245 | None:intergenic |
| GTCGGTTGTGGATCAATCCT+CGG | 0.549786 | 5.4:-64976718 | MS.gene80687:CDS |
| CAACTATTCTCAGGATGTAT+CGG | 0.549930 | 5.4:+64978009 | None:intergenic |
| AAAAGCCAGAAGTACAAAGA+AGG | 0.556508 | 5.4:-64976589 | MS.gene80687:CDS |
| AGTGCTTAAGCATGGTATTG+TGG | 0.559466 | 5.4:-64978067 | None:intergenic |
| TTTCCACAGCAGACATGGGA+AGG | 0.566067 | 5.4:-64977111 | MS.gene80687:CDS |
| AAGGAAAATCTGTTCTTGAA+CGG | 0.572251 | 5.4:-64976570 | MS.gene80687:intron |
| GTTGTGGATCAATCCTCGGT+TGG | 0.576717 | 5.4:-64976714 | MS.gene80687:CDS |
| CTTCTGTGCAAAAGGAAAGG+TGG | 0.584548 | 5.4:-64977611 | MS.gene80687:intron |
| TAAGCATGGTATTGTGGCAA+TGG | 0.593376 | 5.4:-64978061 | None:intergenic |
| AGGCTTTCCACAGCAGACAT+GGG | 0.597650 | 5.4:-64977115 | MS.gene80687:CDS |
| ATGGTTGATGACCGCTGCCA+TGG | 0.599421 | 5.4:+64977952 | None:intergenic |
| AAGCCTTCCCATGTCTGCTG+TGG | 0.600078 | 5.4:+64977108 | None:intergenic |
| TGGGTTAGCCATGCAACACA+TGG | 0.601436 | 5.4:-64976253 | MS.gene80687:CDS |
| CCGAAACAGCACCATGGCAG+CGG | 0.605323 | 5.4:-64977963 | MS.gene80687:CDS |
| AGGACAGAAATATGATGACT+GGG | 0.607783 | 5.4:-64976768 | MS.gene80687:CDS |
| TGTACTTCAGAATTAGACAA+TGG | 0.623278 | 5.4:+64977557 | None:intergenic |
| GAAGGCTTATCTCTTCCGTA+AGG | 0.633340 | 5.4:-64977093 | MS.gene80687:CDS |
| CATCCTGAGAATAGTTGACG+CGG | 0.682429 | 5.4:-64978003 | MS.gene80687:CDS |
| GTCTTACATATGTCCAACCG+AGG | 0.682634 | 5.4:+64976701 | None:intergenic |
| AAACTGCCGAAACAGCACCA+TGG | 0.699071 | 5.4:-64977969 | MS.gene80687:CDS |
| GATGACTGGGATGCATACTG+TGG | 0.714746 | 5.4:-64976755 | MS.gene80687:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTTTTCTTAATGTTTTAA+CGG | - | chr5.4:64977051-64977070 | MS.gene80687:intron | 15.0% |
| !!! | ATTAAGAAAAAGTTGAATTT+TGG | + | chr5.4:64977044-64977063 | None:intergenic | 15.0% |
| !!! | ATTGATAATAATTAGAACAA+GGG | - | chr5.4:64977843-64977862 | MS.gene80687:intron | 15.0% |
| !! | ATATGTATATATATTCTGAC+TGG | - | chr5.4:64977118-64977137 | MS.gene80687:intron | 20.0% |
| !! | TATGATATTTCCATTTAAAC+CGG | + | chr5.4:64976984-64977003 | None:intergenic | 20.0% |
| !!! | ACAGTTATGTTTTTTATTCA+TGG | - | chr5.4:64977367-64977386 | MS.gene80687:intron | 20.0% |
| !!! | CATTGATAATAATTAGAACA+AGG | - | chr5.4:64977842-64977861 | MS.gene80687:intron | 20.0% |
| !!! | TGCTATTAACTAAAACTTTT+TGG | - | chr5.4:64976550-64976569 | MS.gene80687:intron | 20.0% |
| ! | ACTAACTGCAAAATATAATG+CGG | + | chr5.4:64977424-64977443 | None:intergenic | 25.0% |
| ! | AGATGAAAGACAATAACATA+AGG | + | chr5.4:64976421-64976440 | None:intergenic | 25.0% |
| ! | TAAGCATTTATAAGTTTCCA+AGG | + | chr5.4:64976480-64976499 | None:intergenic | 25.0% |
| !! | AAACCATTCTATTCTGATTT+TGG | - | chr5.4:64976896-64976915 | MS.gene80687:intron | 25.0% |
| !!! | ATAAACGACCAAGTTTTTTA+GGG | - | chr5.4:64976958-64976977 | MS.gene80687:intron | 25.0% |
| !!! | ATGTTTTTTATTCATGGCTT+TGG | - | chr5.4:64977373-64977392 | MS.gene80687:intron | 25.0% |
| !!! | GTTTTAACGGTTTTTTTAGT+TGG | - | chr5.4:64977064-64977083 | MS.gene80687:intron | 25.0% |
| !!! | TATAAACGACCAAGTTTTTT+AGG | - | chr5.4:64976957-64976976 | MS.gene80687:intron | 25.0% |
| !!! | TGTTTTTTATTCATGGCTTT+GGG | - | chr5.4:64977374-64977393 | MS.gene80687:intron | 25.0% |
| AAGGAAAATCTGTTCTTGAA+CGG | - | chr5.4:64977711-64977730 | MS.gene80687:intron | 30.0% | |
| AATCTACATAATTCACTGAG+TGG | - | chr5.4:64976844-64976863 | MS.gene80687:intron | 30.0% | |
| ACCTTTGATTACAAACTACA+AGG | + | chr5.4:64977337-64977356 | None:intergenic | 30.0% | |
| AGACAATAACATAAGGTTGA+AGG | + | chr5.4:64976414-64976433 | None:intergenic | 30.0% | |
| CATTAAGTTAAGTTACCCTT+TGG | - | chr5.4:64977644-64977663 | MS.gene80687:intron | 30.0% | |
| GAACCAAAATCAGAATAGAA+TGG | + | chr5.4:64976902-64976921 | None:intergenic | 30.0% | |
| GATTTCGGTTTCATTGAAAA+TGG | + | chr5.4:64977277-64977296 | None:intergenic | 30.0% | |
| GTGAATTATGTAGATTGTAG+AGG | + | chr5.4:64976840-64976859 | None:intergenic | 30.0% | |
| TGGAATCGACATTTCTTTAT+TGG | - | chr5.4:64977084-64977103 | MS.gene80687:intron | 30.0% | |
| TGGTTTCATATATTTCACAG+AGG | - | chr5.4:64976864-64976883 | MS.gene80687:intron | 30.0% | |
| TGTACTTCAGAATTAGACAA+TGG | + | chr5.4:64976727-64976746 | None:intergenic | 30.0% | |
| TTTGTGTACCAATACACAAA+TGG | + | chr5.4:64976351-64976370 | None:intergenic | 30.0% | |
| ! | ATTTTCCTTCTTTGTACTTC+TGG | + | chr5.4:64977700-64977719 | None:intergenic | 30.0% |
| ! | GTTTGTAATCAAAGGTGTAA+AGG | - | chr5.4:64977341-64977360 | MS.gene80687:intron | 30.0% |
| ! | TACAGTGTGAATGTTTCTAT+TGG | - | chr5.4:64977482-64977501 | MS.gene80687:intron | 30.0% |
| ! | TGTTTCTATTGGAGAATTAG+AGG | - | chr5.4:64977493-64977512 | MS.gene80687:intron | 30.0% |
| ACTTAATGCAGCATAGAAAC+AGG | + | chr5.4:64977632-64977651 | None:intergenic | 35.0% | |
| AGAAGTTACTTCTGTGCAAA+AGG | - | chr5.4:64976662-64976681 | MS.gene80687:intron | 35.0% | |
| AGCGATATATAAGTATGTCG+TGG | - | chr5.4:64977944-64977963 | MS.gene80687:CDS | 35.0% | |
| AGGACAGAAATATGATGACT+GGG | - | chr5.4:64977513-64977532 | MS.gene80687:intron | 35.0% | |
| ATGCAGGACAAAAGGTTAAA+GGG | + | chr5.4:64977305-64977324 | None:intergenic | 35.0% | |
| ATGGTGAAACCAAAGTTATC+AGG | + | chr5.4:64977258-64977277 | None:intergenic | 35.0% | |
| CAACTATTCTCAGGATGTAT+CGG | + | chr5.4:64976275-64976294 | None:intergenic | 35.0% | |
| CAAGGATAACTTACACCTTA+CGG | + | chr5.4:64977206-64977225 | None:intergenic | 35.0% | |
| CAATAACATAAGGTTGAAGG+TGG | + | chr5.4:64976411-64976430 | None:intergenic | 35.0% | |
| CAATGGTGCAAGATACAAAA+TGG | - | chr5.4:64976237-64976256 | MS.gene80687:CDS | 35.0% | |
| GCAAATAGTCCTTCAAAAGT+AGG | + | chr5.4:64976703-64976722 | None:intergenic | 35.0% | |
| GTCATCAACCATTTGTGTAT+TGG | - | chr5.4:64976340-64976359 | MS.gene80687:intron | 35.0% | |
| GTGTAAGTTATCCTTGACAT+TGG | - | chr5.4:64977210-64977229 | MS.gene80687:intron | 35.0% | |
| TATGAGTGTATCTGACAAAG+TGG | - | chr5.4:64976591-64976610 | MS.gene80687:CDS | 35.0% | |
| TGAAGGTGGTCATTAAATCA+AGG | + | chr5.4:64976397-64976416 | None:intergenic | 35.0% | |
| TTACTTCTGTGCAAAAGGAA+AGG | - | chr5.4:64976667-64976686 | MS.gene80687:intron | 35.0% | |
| ! | AAAAGCCAGAAGTACAAAGA+AGG | - | chr5.4:64977692-64977711 | MS.gene80687:intron | 35.0% |
| ! | CTTTTGTCCTGCATCATTTT+GGG | - | chr5.4:64977311-64977330 | MS.gene80687:intron | 35.0% |
| ! | GCCTTGTAGTTTGTAATCAA+AGG | - | chr5.4:64977333-64977352 | MS.gene80687:intron | 35.0% |
| !! | GGAAGTAATCCTACTTTTGA+AGG | - | chr5.4:64976691-64976710 | MS.gene80687:intron | 35.0% |
| !! | GTTTTGGCATTAGCACAATT+TGG | + | chr5.4:64976447-64976466 | None:intergenic | 35.0% |
| !! | TTACTTGTGCTGTTTCATTC+AGG | - | chr5.4:64977146-64977165 | MS.gene80687:intron | 35.0% |
| !!! | TTTTTTAGGGCCGGTTTAAA+TGG | - | chr5.4:64976971-64976990 | MS.gene80687:intron | 35.0% |
| AGTCTCCTGAAAGAAACCAA+AGG | + | chr5.4:64977663-64977682 | None:intergenic | 40.0% | |
| CCAAAACATGTATGCTTCCT+TGG | - | chr5.4:64976460-64976479 | MS.gene80687:intron | 40.0% | |
| CCAAAATGATGCAGGACAAA+AGG | + | chr5.4:64977313-64977332 | None:intergenic | 40.0% | |
| GAGGACAGAAATATGATGAC+TGG | - | chr5.4:64977512-64977531 | MS.gene80687:intron | 40.0% | |
| GATGCAGGACAAAAGGTTAA+AGG | + | chr5.4:64977306-64977325 | None:intergenic | 40.0% | |
| GGTTAAAGGGTGGAAGATTT+CGG | + | chr5.4:64977292-64977311 | None:intergenic | 40.0% | |
| GTCTCCTGAAAGAAACCAAA+GGG | + | chr5.4:64977662-64977681 | None:intergenic | 40.0% | |
| GTGTTCTGTCCTGATAACTT+TGG | - | chr5.4:64977246-64977265 | MS.gene80687:intron | 40.0% | |
| TAAACCGGCCCTAAAAAACT+TGG | + | chr5.4:64976969-64976988 | None:intergenic | 40.0% | |
| TAATGCAGCATAGAAACAGG+AGG | + | chr5.4:64977629-64977648 | None:intergenic | 40.0% | |
| TAATTACTCTGTCAGTGTCG+TGG | - | chr5.4:64977879-64977898 | MS.gene80687:intron | 40.0% | |
| TATAATGCGGCTAGAGCTAA+TGG | + | chr5.4:64977411-64977430 | None:intergenic | 40.0% | |
| TATATCGCTCAAAACCCATG+TGG | + | chr5.4:64977933-64977952 | None:intergenic | 40.0% | |
| TCCTGATGGCAGCAATTATT+GGG | - | chr5.4:64978009-64978028 | MS.gene80687:CDS | 40.0% | |
| TGCCAAACATCAACACATGT+CGG | - | chr5.4:64977905-64977924 | MS.gene80687:intron | 40.0% | |
| TTTCAACACCATGTGTTGCA+TGG | + | chr5.4:64978039-64978058 | None:intergenic | 40.0% | |
| ! | CCTTTTGTCCTGCATCATTT+TGG | - | chr5.4:64977310-64977329 | MS.gene80687:intron | 40.0% |
| ! | GAGTTTTTGCTCTTTTGACC+AGG | - | chr5.4:64977972-64977991 | MS.gene80687:CDS | 40.0% |
| ! | GTTACCCTTTGGTTTCTTTC+AGG | - | chr5.4:64977655-64977674 | MS.gene80687:intron | 40.0% |
| !! | AGTGAAAGGTACCAATGTCA+AGG | + | chr5.4:64977224-64977243 | None:intergenic | 40.0% |
| !! | CCAAGGAAGCATACATGTTT+TGG | + | chr5.4:64976463-64976482 | None:intergenic | 40.0% |
| ACCCAATAATTGCTGCCATC+AGG | + | chr5.4:64978013-64978032 | None:intergenic | 45.0% | |
| AGGACAGAACACGTAGTGAA+AGG | + | chr5.4:64977238-64977257 | None:intergenic | 45.0% | |
| CAGGACAAAAGGTTAAAGGG+TGG | + | chr5.4:64977302-64977321 | None:intergenic | 45.0% | |
| CATCCTGAGAATAGTTGACG+CGG | - | chr5.4:64976278-64976297 | MS.gene80687:CDS | 45.0% | |
| CTTCTGTGCAAAAGGAAAGG+TGG | - | chr5.4:64976670-64976689 | MS.gene80687:intron | 45.0% | |
| GAAGGCTTATCTCTTCCGTA+AGG | - | chr5.4:64977188-64977207 | MS.gene80687:intron | 45.0% | |
| GGCTAGAGCTAATGGAGATT+CGG | + | chr5.4:64977403-64977422 | None:intergenic | 45.0% | |
| GTCCTGATGGCAGCAATTAT+TGG | - | chr5.4:64978008-64978027 | MS.gene80687:CDS | 45.0% | |
| GTCTTACATATGTCCAACCG+AGG | + | chr5.4:64977583-64977602 | None:intergenic | 45.0% | |
| TACAAGGCCCAAAATGATGC+AGG | + | chr5.4:64977321-64977340 | None:intergenic | 45.0% | |
| TAGAGCTAATGGAGATTCGG+AGG | + | chr5.4:64977400-64977419 | None:intergenic | 45.0% | |
| ! | CACCGACATGTGTTGATGTT+TGG | + | chr5.4:64977910-64977929 | None:intergenic | 45.0% |
| ! | GTGGCTGACATTTTCTGTGT+CGG | - | chr5.4:64977545-64977564 | MS.gene80687:intron | 45.0% |
| ! | TGCTCTTTTGACCAGGTTCA+AGG | - | chr5.4:64977979-64977998 | MS.gene80687:CDS | 45.0% |
| !!! | ACGACCAAGTTTTTTAGGGC+CGG | - | chr5.4:64976962-64976981 | MS.gene80687:intron | 45.0% |
| !!! | GACATTTTCTGTGTCGGTTG+TGG | - | chr5.4:64977551-64977570 | MS.gene80687:intron | 45.0% |
| AAACTGCCGAAACAGCACCA+TGG | - | chr5.4:64976312-64976331 | MS.gene80687:intron | 50.0% | |
| AGGCTTTCCACAGCAGACAT+GGG | - | chr5.4:64977166-64977185 | MS.gene80687:intron | 50.0% | |
| CTACCGCGTCAACTATTCTC+AGG | + | chr5.4:64976284-64976303 | None:intergenic | 50.0% | |
| GAGAATAGTTGACGCGGTAG+TGG | - | chr5.4:64976284-64976303 | MS.gene80687:CDS | 50.0% | |
| GATGACTGGGATGCATACTG+TGG | - | chr5.4:64977526-64977545 | MS.gene80687:intron | 50.0% | |
| GTCGGTTGTGGATCAATCCT+CGG | - | chr5.4:64977563-64977582 | MS.gene80687:CDS | 50.0% | |
| GTTGTGGATCAATCCTCGGT+TGG | - | chr5.4:64977567-64977586 | MS.gene80687:CDS | 50.0% | |
| TTTCCACAGCAGACATGGGA+AGG | - | chr5.4:64977170-64977189 | MS.gene80687:intron | 50.0% | |
| ! | TTCAAGGTGTCTGGTCCTGA+TGG | - | chr5.4:64977995-64978014 | MS.gene80687:CDS | 50.0% |
| ! | TTGACCAGGTTCAAGGTGTC+TGG | - | chr5.4:64977986-64978005 | MS.gene80687:CDS | 50.0% |
| !! | AACACATGTCGGTGCCACAT+GGG | - | chr5.4:64977916-64977935 | MS.gene80687:intron | 50.0% |
| !! | TGGGTTAGCCATGCAACACA+TGG | - | chr5.4:64978028-64978047 | MS.gene80687:CDS | 50.0% |
| !! | TGGTGCTGTTTCGGCAGTTT+CGG | + | chr5.4:64976312-64976331 | None:intergenic | 50.0% |
| AAGCCTTCCCATGTCTGCTG+TGG | + | chr5.4:64977176-64977195 | None:intergenic | 55.0% | |
| AGGACCAGACACCTTGAACC+TGG | + | chr5.4:64977993-64978012 | None:intergenic | 55.0% | |
| CAGGCTTTCCACAGCAGACA+TGG | - | chr5.4:64977165-64977184 | MS.gene80687:intron | 55.0% | |
| !! | ATGGTTGATGACCGCTGCCA+TGG | + | chr5.4:64976332-64976351 | None:intergenic | 55.0% |
| !! | CAACACATGTCGGTGCCACA+TGG | - | chr5.4:64977915-64977934 | MS.gene80687:intron | 55.0% |
| CCGAAACAGCACCATGGCAG+CGG | - | chr5.4:64976318-64976337 | MS.gene80687:intron | 60.0% | |
| !! | CCGCTGCCATGGTGCTGTTT+CGG | + | chr5.4:64976321-64976340 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 64976225 | 64978078 | 64976225 | ID=MS.gene80687 |
| chr5.4 | mRNA | 64976225 | 64978078 | 64976225 | ID=MS.gene80687.t1;Parent=MS.gene80687 |
| chr5.4 | exon | 64977942 | 64978078 | 64977942 | ID=MS.gene80687.t1.exon1;Parent=MS.gene80687.t1 |
| chr5.4 | CDS | 64977942 | 64978078 | 64977942 | ID=cds.MS.gene80687.t1;Parent=MS.gene80687.t1 |
| chr5.4 | exon | 64977562 | 64977619 | 64977562 | ID=MS.gene80687.t1.exon2;Parent=MS.gene80687.t1 |
| chr5.4 | CDS | 64977562 | 64977619 | 64977562 | ID=cds.MS.gene80687.t1;Parent=MS.gene80687.t1 |
| chr5.4 | exon | 64977092 | 64977135 | 64977092 | ID=MS.gene80687.t1.exon3;Parent=MS.gene80687.t1 |
| chr5.4 | CDS | 64977092 | 64977135 | 64977092 | ID=cds.MS.gene80687.t1;Parent=MS.gene80687.t1 |
| chr5.4 | exon | 64976708 | 64976816 | 64976708 | ID=MS.gene80687.t1.exon4;Parent=MS.gene80687.t1 |
| chr5.4 | CDS | 64976708 | 64976816 | 64976708 | ID=cds.MS.gene80687.t1;Parent=MS.gene80687.t1 |
| chr5.4 | exon | 64976571 | 64976626 | 64976571 | ID=MS.gene80687.t1.exon5;Parent=MS.gene80687.t1 |
| chr5.4 | CDS | 64976571 | 64976626 | 64976571 | ID=cds.MS.gene80687.t1;Parent=MS.gene80687.t1 |
| chr5.4 | exon | 64976225 | 64976309 | 64976225 | ID=MS.gene80687.t1.exon6;Parent=MS.gene80687.t1 |
| chr5.4 | CDS | 64976225 | 64976309 | 64976225 | ID=cds.MS.gene80687.t1;Parent=MS.gene80687.t1 |
| Gene Sequence |
| Protein sequence |
