AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene98609


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98609.t1 MTR_4g052010 97.872 188 4 0 1 188 1 188 2.35e-133 371
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98609.t1 AT2G27290 61.658 193 54 4 4 181 3 190 4.97e-62 191

Find 36 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTCTTGGTTACTTCTTC+AGG 0.280999 4.2:-50140703 None:intergenic
ACAAAGCTGCATCTCCAATT+AGG 0.288679 4.2:+50142032 MS.gene98609:CDS
AAGGAGTTGCTGGCAAAATA+TGG 0.314060 4.2:+50141749 MS.gene98609:CDS
AGCTTCAAGACCATACTTCT+TGG 0.339383 4.2:-50140716 None:intergenic
ATGGTCTTGAAGCTGGTCTT+TGG 0.345812 4.2:+50140725 MS.gene98609:CDS
ACTGTGGGTGGAAACCTAAT+TGG 0.379765 4.2:-50142046 None:intergenic
TGATCAAGCCAAGGAGTTGC+TGG 0.407305 4.2:+50141739 MS.gene98609:CDS
CAATCCAACCAGCTACGATT+GGG 0.413841 4.2:-50142078 None:intergenic
AAAAGTCGGTACCTTTGCTT+TGG 0.429669 4.2:+50141997 MS.gene98609:CDS
GAAGAAGTAACCAAGAAGTA+TGG 0.441811 4.2:+50140706 MS.gene98609:CDS
ATTAGATTTACGTGACTGAA+TGG 0.446882 4.2:-50140608 None:intergenic
CCAATCCAACCAGCTACGAT+TGG 0.459512 4.2:-50142079 None:intergenic
TGTTATGTATTAATCAATGC+TGG 0.464473 4.2:+50141824 MS.gene98609:CDS
CCAATCGTAGCTGGTTGGAT+TGG 0.470086 4.2:+50142079 MS.gene98609:CDS
TTCAGGTGAAGAATTCTTTG+AGG 0.474321 4.2:-50140686 None:intergenic
AAGAAGTATGGTCTTGAAGC+TGG 0.499317 4.2:+50140718 MS.gene98609:CDS
TGTTCAAACTTTGCTTCAGA+AGG 0.506472 4.2:+50141853 MS.gene98609:CDS
GCTCTTACCCCAATCGTAGC+TGG 0.519207 4.2:+50142070 MS.gene98609:CDS
GGGGAACAACAGAAATCAAA+AGG 0.520589 4.2:+50141716 MS.gene98609:CDS
TTACCCCAATCGTAGCTGGT+TGG 0.540207 4.2:+50142074 MS.gene98609:CDS
TTTCAGTTCAAAGGAAGAAG+GGG 0.549822 4.2:+50141697 MS.gene98609:CDS
AGAGATCAATGACAGAGTAA+TGG 0.561630 4.2:-50141792 None:intergenic
TCTTGAAGCTGGTCTTTGGA+AGG 0.564484 4.2:+50140729 MS.gene98609:CDS
AAAGAAGCACAAGAAGGTGA+TGG 0.588002 4.2:-50140544 None:intergenic
CTCAGAAAAGAAGCACAAGA+AGG 0.593610 4.2:-50140550 None:intergenic
ATCAAAAGGTGATCAAGCCA+AGG 0.614436 4.2:+50141730 MS.gene98609:CDS
GGGGTAAGAGCTACTGTGGG+TGG 0.621082 4.2:-50142058 None:intergenic
GATCAATGACAGAGTAATGG+AGG 0.626103 4.2:-50141789 None:intergenic
CAATGACAGAGTAATGGAGG+TGG 0.627479 4.2:-50141786 None:intergenic
GGAATCACTACTGATGCTAC+CGG 0.632753 4.2:+50141971 MS.gene98609:CDS
TGCAGCATATGCCAAAGCAA+AGG 0.641846 4.2:-50142008 None:intergenic
GATTGGGGTAAGAGCTACTG+TGG 0.646696 4.2:-50142062 None:intergenic
AATCCAACCAGCTACGATTG+GGG 0.648062 4.2:-50142077 None:intergenic
GAGTTGCTGGCAAAATATGG+AGG 0.682334 4.2:+50141752 MS.gene98609:CDS
ATTGGGGTAAGAGCTACTGT+GGG 0.725389 4.2:-50142061 None:intergenic
AGCTGTTAAAGAGAAAACTG+AGG 0.740712 4.2:+50140651 MS.gene98609:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATGAAGGAAAAAAAAA+AGG - chr4.2:50140892-50140911 None:intergenic 15.0%
!! ATAAAACCTAAAATAAAACT+CGG - chr4.2:50141183-50141202 None:intergenic 15.0%
!!! ATTTAAAGCAATAAAAATGA+AGG - chr4.2:50140904-50140923 None:intergenic 15.0%
!!! GTTCTAAAAAGTATATATAA+AGG + chr4.2:50140958-50140977 MS.gene98609:intron 15.0%
!! AAATAAACAAGAGAAAGAAA+TGG - chr4.2:50140758-50140777 None:intergenic 20.0%
!! AATAAACAAGAGAAAGAAAT+GGG - chr4.2:50140757-50140776 None:intergenic 20.0%
!! ATTATACTTAAATCCACATT+GGG - chr4.2:50141897-50141916 None:intergenic 20.0%
!! TATAAAGGATATTCAAAGAT+AGG + chr4.2:50140973-50140992 MS.gene98609:intron 20.0%
!! TCTGTGTAAAATGAAAATTA+TGG + chr4.2:50141662-50141681 MS.gene98609:intron 20.0%
!! TTCAAACTTCTACTTTAAAT+TGG - chr4.2:50141357-50141376 None:intergenic 20.0%
!! TTGGTTTAATTATGATTATG+TGG + chr4.2:50140777-50140796 MS.gene98609:intron 20.0%
!!! GAAATATAAGTTGAGTTTTA+AGG + chr4.2:50141439-50141458 MS.gene98609:intron 20.0%
!!! TTTCTTTCTCTTGTTTATTT+TGG + chr4.2:50140758-50140777 MS.gene98609:intron 20.0%
! AAAAGATGAATTTCTCACTT+AGG - chr4.2:50140829-50140848 None:intergenic 25.0%
! ACTAATGATTGAAAATCGTT+TGG - chr4.2:50141585-50141604 None:intergenic 25.0%
! CATTATACTTAAATCCACAT+TGG - chr4.2:50141898-50141917 None:intergenic 25.0%
! TGTTATGTATTAATCAATGC+TGG + chr4.2:50141824-50141843 MS.gene98609:CDS 25.0%
!! AAGTGAGAAATTCATCTTTT+TGG + chr4.2:50140829-50140848 MS.gene98609:intron 25.0%
!! AGTGAGAAATTCATCTTTTT+GGG + chr4.2:50140830-50140849 MS.gene98609:intron 25.0%
!!! TAGGTTTTATTTCCATTTCT+TGG + chr4.2:50141193-50141212 MS.gene98609:intron 25.0%
!!! TTGTTTCCGAGTTTTATTTT+AGG + chr4.2:50141174-50141193 MS.gene98609:intron 25.0%
ATTAGATTTACGTGACTGAA+TGG - chr4.2:50140611-50140630 None:intergenic 30.0%
TATCTCAGCAGTAAAATTGT+TGG - chr4.2:50141383-50141402 None:intergenic 30.0%
TTTCTTGTCATGTAGAACTT+GGG + chr4.2:50141088-50141107 MS.gene98609:intron 30.0%
! ATTTTATTGCCGAAATTTGC+AGG + chr4.2:50140996-50141015 MS.gene98609:intron 30.0%
! ATTTTCAGTTCAAAGGAAGA+AGG + chr4.2:50141695-50141714 MS.gene98609:CDS 30.0%
! TGTATCTTTCCCTGATTTTA+GGG + chr4.2:50141226-50141245 MS.gene98609:intron 30.0%
! TTTGCAGATTTTCAGTTCAA+AGG + chr4.2:50141688-50141707 MS.gene98609:intron 30.0%
! TTTTATTGCCGAAATTTGCA+GGG + chr4.2:50140997-50141016 MS.gene98609:intron 30.0%
! TTTTCAGTTCAAAGGAAGAA+GGG + chr4.2:50141696-50141715 MS.gene98609:CDS 30.0%
! TTTTCTTGTCATGTAGAACT+TGG + chr4.2:50141087-50141106 MS.gene98609:intron 30.0%
!! GACATGGGTTTTATACTTAT+GGG + chr4.2:50141536-50141555 MS.gene98609:intron 30.0%
!! TGACATGGGTTTTATACTTA+TGG + chr4.2:50141535-50141554 MS.gene98609:intron 30.0%
AGAGATCAATGACAGAGTAA+TGG - chr4.2:50141795-50141814 None:intergenic 35.0%
AGCTGTTAAAGAGAAAACTG+AGG + chr4.2:50140651-50140670 MS.gene98609:CDS 35.0%
CAGTACAGAACATTTCTTGA+AGG - chr4.2:50141283-50141302 None:intergenic 35.0%
CAGTATGCTAAACCAAGAAA+TGG - chr4.2:50141208-50141227 None:intergenic 35.0%
CATTAAAACGATAACCCAAC+TGG - chr4.2:50141501-50141520 None:intergenic 35.0%
CATTATGTCTGTATATCTGC+AGG + chr4.2:50141946-50141965 MS.gene98609:intron 35.0%
GAAGAAGTAACCAAGAAGTA+TGG + chr4.2:50140706-50140725 MS.gene98609:CDS 35.0%
GAGAGGATGAATAATGACAT+GGG + chr4.2:50141521-50141540 MS.gene98609:intron 35.0%
TACTTCTTGGTTACTTCTTC+AGG - chr4.2:50140706-50140725 None:intergenic 35.0%
TGTTCAAACTTTGCTTCAGA+AGG + chr4.2:50141853-50141872 MS.gene98609:CDS 35.0%
TTCAGGTGAAGAATTCTTTG+AGG - chr4.2:50140689-50140708 None:intergenic 35.0%
TTTACACAGAATGAAAAGCG+CGG - chr4.2:50141652-50141671 None:intergenic 35.0%
TTTCAGTTCAAAGGAAGAAG+GGG + chr4.2:50141697-50141716 MS.gene98609:CDS 35.0%
TTTCCTTTCCCTGCAAATTT+CGG - chr4.2:50141008-50141027 None:intergenic 35.0%
! ATTTTGCTCCCCTAAAATCA+GGG - chr4.2:50141238-50141257 None:intergenic 35.0%
! CAGTTGGGTTATCGTTTTAA+TGG + chr4.2:50141499-50141518 MS.gene98609:intron 35.0%
! CTGTATCTTTCCCTGATTTT+AGG + chr4.2:50141225-50141244 MS.gene98609:intron 35.0%
! GTATCTTTCCCTGATTTTAG+GGG + chr4.2:50141227-50141246 MS.gene98609:intron 35.0%
! TATTTTGCTCCCCTAAAATC+AGG - chr4.2:50141239-50141258 None:intergenic 35.0%
AAAAGTCGGTACCTTTGCTT+TGG + chr4.2:50141997-50142016 MS.gene98609:CDS 40.0%
AAATTGGCGACACAAGTAGT+CGG - chr4.2:50141341-50141360 None:intergenic 40.0%
AAGGAGTTGCTGGCAAAATA+TGG + chr4.2:50141749-50141768 MS.gene98609:CDS 40.0%
ACAAAGCTGCATCTCCAATT+AGG + chr4.2:50142032-50142051 MS.gene98609:CDS 40.0%
AGCTTCAAGACCATACTTCT+TGG - chr4.2:50140719-50140738 None:intergenic 40.0%
ATCAAAAGGTGATCAAGCCA+AGG + chr4.2:50141730-50141749 MS.gene98609:CDS 40.0%
ATGTCTGTATATCTGCAGGT+AGG + chr4.2:50141950-50141969 MS.gene98609:intron 40.0%
CGGAATCGATATGCAAACAT+TGG - chr4.2:50141321-50141340 None:intergenic 40.0%
GATCAATGACAGAGTAATGG+AGG - chr4.2:50141792-50141811 None:intergenic 40.0%
GGAGAGGATGAATAATGACA+TGG + chr4.2:50141520-50141539 MS.gene98609:intron 40.0%
GGGAGCAAAATATGTGTTGT+GGG + chr4.2:50141247-50141266 MS.gene98609:intron 40.0%
GGGGAACAACAGAAATCAAA+AGG + chr4.2:50141716-50141735 MS.gene98609:CDS 40.0%
GGTTGAGTCGACAAAAATCA+AGG - chr4.2:50141480-50141499 None:intergenic 40.0%
TGCTCATACATAACCCAATG+TGG + chr4.2:50141881-50141900 MS.gene98609:intron 40.0%
! AAAGAAGCACAAGAAGGTGA+TGG - chr4.2:50140547-50140566 None:intergenic 40.0%
! AAGAAGTATGGTCTTGAAGC+TGG + chr4.2:50140718-50140737 MS.gene98609:CDS 40.0%
! ATATTTTGCCAGCAACTCCT+TGG - chr4.2:50141750-50141769 None:intergenic 40.0%
! CTCAGAAAAGAAGCACAAGA+AGG - chr4.2:50140553-50140572 None:intergenic 40.0%
! GAAGTGTCTCGTATAGTCTA+CGG - chr4.2:50141611-50141630 None:intergenic 40.0%
!! GGGTTATCGTTTTAATGGAG+AGG + chr4.2:50141504-50141523 MS.gene98609:intron 40.0%
!!! AAAGGTACCGACTTTTTCAC+CGG - chr4.2:50141993-50142012 None:intergenic 40.0%
!!! TTTTGTCGACTCAACCAGTT+GGG + chr4.2:50141484-50141503 MS.gene98609:intron 40.0%
!!! TTTTTGTCGACTCAACCAGT+TGG + chr4.2:50141483-50141502 MS.gene98609:intron 40.0%
AATCCAACCAGCTACGATTG+GGG - chr4.2:50142080-50142099 None:intergenic 45.0%
ACTGTGGGTGGAAACCTAAT+TGG - chr4.2:50142049-50142068 None:intergenic 45.0%
ATTGGGGTAAGAGCTACTGT+GGG - chr4.2:50142064-50142083 None:intergenic 45.0%
CAATCCAACCAGCTACGATT+GGG - chr4.2:50142081-50142100 None:intergenic 45.0%
GAGTTGCTGGCAAAATATGG+AGG + chr4.2:50141752-50141771 MS.gene98609:CDS 45.0%
GATGCTACCGGTGAAAAAGT+CGG + chr4.2:50141983-50142002 MS.gene98609:CDS 45.0%
GGAATCACTACTGATGCTAC+CGG + chr4.2:50141971-50141990 MS.gene98609:CDS 45.0%
GGGGAGCAAAATATGTGTTG+TGG + chr4.2:50141246-50141265 MS.gene98609:intron 45.0%
TGCAGCATATGCCAAAGCAA+AGG - chr4.2:50142011-50142030 None:intergenic 45.0%
TTGCCGAAATTTGCAGGGAA+AGG + chr4.2:50141002-50141021 MS.gene98609:intron 45.0%
! AAAAGCGCGGCTTTGACTTT+AGG - chr4.2:50141639-50141658 None:intergenic 45.0%
! AAAGCGCGGCTTTGACTTTA+GGG - chr4.2:50141638-50141657 None:intergenic 45.0%
! CAATGACAGAGTAATGGAGG+TGG - chr4.2:50141789-50141808 None:intergenic 45.0%
!! ATGGTCTTGAAGCTGGTCTT+TGG + chr4.2:50140725-50140744 MS.gene98609:CDS 45.0%
!! TCTTGAAGCTGGTCTTTGGA+AGG + chr4.2:50140729-50140748 MS.gene98609:CDS 45.0%
!! TTATGGGAGTGTCATCGTGT+CGG + chr4.2:50141552-50141571 MS.gene98609:intron 45.0%
CCAATCCAACCAGCTACGAT+TGG - chr4.2:50142082-50142101 None:intergenic 50.0%
CCAATCGTAGCTGGTTGGAT+TGG + chr4.2:50142079-50142098 MS.gene98609:CDS 50.0%
GATTGGGGTAAGAGCTACTG+TGG - chr4.2:50142065-50142084 None:intergenic 50.0%
TGATCAAGCCAAGGAGTTGC+TGG + chr4.2:50141739-50141758 MS.gene98609:CDS 50.0%
TTACCCCAATCGTAGCTGGT+TGG + chr4.2:50142074-50142093 MS.gene98609:CDS 50.0%
!! GCGGCTTTGACTTTAGGGTT+AGG - chr4.2:50141633-50141652 None:intergenic 50.0%
GCTCTTACCCCAATCGTAGC+TGG + chr4.2:50142070-50142089 MS.gene98609:CDS 55.0%
GGGGTAAGAGCTACTGTGGG+TGG - chr4.2:50142061-50142080 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 50140523 50142126 50140523 ID=MS.gene98609
chr4.2 mRNA 50140523 50142126 50140523 ID=MS.gene98609.t1;Parent=MS.gene98609
chr4.2 exon 50140523 50140750 50140523 ID=MS.gene98609.t1.exon1;Parent=MS.gene98609.t1
chr4.2 CDS 50140523 50140750 50140523 ID=cds.MS.gene98609.t1;Parent=MS.gene98609.t1
chr4.2 exon 50141695 50141874 50141695 ID=MS.gene98609.t1.exon2;Parent=MS.gene98609.t1
chr4.2 CDS 50141695 50141874 50141695 ID=cds.MS.gene98609.t1;Parent=MS.gene98609.t1
chr4.2 exon 50141968 50142126 50141968 ID=MS.gene98609.t1.exon3;Parent=MS.gene98609.t1
chr4.2 CDS 50141968 50142126 50141968 ID=cds.MS.gene98609.t1;Parent=MS.gene98609.t1
Gene Sequence

>MS.gene98609

ATGACAACGTTTTTGTTGCTACCATCACCTTCTTGTGCTTCTTTTCTGAGCAACAAGAAGAGTTCAAGATTTTGCAGTCTTGCTTCCATTCAGTCACGTAAATCTAATGTTAAAGTGTTGAGAGTTAGAGCTGTTAAAGAGAAAACTGAGGAAATCAAAAGCTCCTCAAAGAATTCTTCACCTGAAGAAGTAACCAAGAAGTATGGTCTTGAAGCTGGTCTTTGGAAGGTGCCCATTTCTTTCTCTTGTTTATTTTGGTTTAATTATGATTATGTGGTTAGTATAAAGTTTCAATTTCATTAACCTAAGTGAGAAATTCATCTTTTTGGGTTGCTTCTTGTCTATGTATCATTTGCTAGCATGCAACCTTTTTTTTTTCCTTCATTTTTATTGCTTTAAATGCAAAGAGTGTATTGTTTTATACTCTATAAATCTGTTCTAAAAAGTATATATAAAGGATATTCAAAGATAGGATTTTATTGCCGAAATTTGCAGGGAAAGGAAATAAAGCTTTTAAAGAAAGAATTTAGTTTGAACTTTGAACAAGTTAGACAAAATGAGTAGTTTTCTTGTCATGTAGAACTTGGGCTGTATCAGATTAAATGTTGCGTTTAAAACAAGAGATATCACTTTATCAGTTATTTCGTGTGTTTGTTTCCGAGTTTTATTTTAGGTTTTATTTCCATTTCTTGGTTTAGCATACTGTATCTTTCCCTGATTTTAGGGGAGCAAAATATGTGTTGTGGGTGAAATTTTACCTTCAAGAAATGTTCTGTACTGAATCAAGATTGAGTACCAATGTTTGCATATCGATTCCGACTACTTGTGTCGCCAATTTAAAGTAGAAGTTTGAATTTCCAACAATTTTACTGCTGAGATAGTAATTTAATCAGTGTTGATTTGTTAAAAATTATCAGAAATATAAGTTGAGTTTTAAGGTAGTGAAATTTGTTTCCTTGATTTTTGTCGACTCAACCAGTTGGGTTATCGTTTTAATGGAGAGGATGAATAATGACATGGGTTTTATACTTATGGGAGTGTCATCGTGTCGGTAGAGCTCCAAACGATTTTCAATCATTAGTTTACCGTAGACTATACGAGACACTTCCTAACCCTAAAGTCAAAGCCGCGCTTTTCATTCTGTGTAAAATGAAAATTATGGTCTTTTGCAGATTTTCAGTTCAAAGGAAGAAGGGGAACAACAGAAATCAAAAGGTGATCAAGCCAAGGAGTTGCTGGCAAAATATGGAGGAGCATACTTAGCCACCTCCATTACTCTGTCATTGATCTCTTTCGCTCTTTGTTATGTATTAATCAATGCTGGAGTAGATGTTCAAACTTTGCTTCAGAAGGTGCAGTGCTCATACATAACCCAATGTGGATTTAAGTATAATGAAAACAAAGTAACTTCTATGAATTAACACATTATGTCTGTATATCTGCAGGTAGGAATCACTACTGATGCTACCGGTGAAAAAGTCGGTACCTTTGCTTTGGCATATGCTGCACACAAAGCTGCATCTCCAATTAGGTTTCCACCCACAGTAGCTCTTACCCCAATCGTAGCTGGTTGGATTGGAAAGAAAGCTGATAAAGATAAGTAG

Protein sequence

>MS.gene98609.t1

MTTFLLLPSPSCASFLSNKKSSRFCSLASIQSRKSNVKVLRVRAVKEKTEEIKSSSKNSSPEEVTKKYGLEAGLWKIFSSKEEGEQQKSKGDQAKELLAKYGGAYLATSITLSLISFALCYVLINAGVDVQTLLQKVGITTDATGEKVGTFALAYAAHKAASPIRFPPTVALTPIVAGWIGKKADKDK