AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54795


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54795.t1 MTR_1g095000 98.286 175 3 0 1 175 106 280 5.86e-127 358
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54795.t1 AT2G39000 74.419 172 44 0 1 172 55 226 5.02e-97 280
MS.gene54795.t1 AT2G39000 74.419 172 44 0 1 172 111 282 9.40e-97 282
MS.gene54795.t1 AT2G39000 77.500 80 18 0 1 80 111 190 4.80e-41 137
MS.gene54795.t1 AT2G39000 77.215 79 18 0 1 79 111 189 2.88e-40 136

Find 43 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGATCAAACTTTCTTATT+TGG 0.151315 1.2:+73150714 MS.gene54795:CDS
ACCTGTCTGGTTGCTGTTAT+TGG 0.195810 1.2:+73150684 MS.gene54795:CDS
TTTCAAATCTATAAAGGTTC+CGG 0.297267 1.2:+73152110 MS.gene54795:CDS
TGTCGCAGAGGCCCCTTTCT+AGG 0.313734 1.2:-73150837 None:intergenic
GCAAGGTTTCAAATCTATAA+AGG 0.323365 1.2:+73152104 MS.gene54795:CDS
GGCACGACATCCCCAACTTC+TGG 0.376576 1.2:-73152028 None:intergenic
AGTGAAGACTTCAAGATTGC+GGG 0.393136 1.2:+73150738 MS.gene54795:CDS
TTCAAATCTATAAAGGTTCC+GGG 0.414198 1.2:+73152111 MS.gene54795:CDS
TGTGTTGAGGATGGATAGAT+TGG 0.415569 1.2:+73150617 None:intergenic
AGAATCACAAGCCAGAAGTT+GGG 0.428773 1.2:+73152017 MS.gene54795:CDS
AGGTTCCGGGAGGAGCTAAC+TGG 0.431391 1.2:+73152124 MS.gene54795:CDS
AGCTAAACAATTGGTGGCTA+AGG 0.435007 1.2:+73151993 MS.gene54795:CDS
TGCAGACTTTCTACCTAGAA+AGG 0.446932 1.2:+73150824 MS.gene54795:CDS
ATGAAGTTGAACTTCACATC+TGG 0.450823 1.2:-73152165 None:intergenic
CAGAATCACAAGCCAGAAGT+TGG 0.456663 1.2:+73152016 MS.gene54795:CDS
GCAGGATTCTCTTTACCAAA+TGG 0.460775 1.2:+73150651 MS.gene54795:CDS
GAAAGGAATAGCTAAACAAT+TGG 0.467703 1.2:+73151984 MS.gene54795:CDS
GCAGACTTTCTACCTAGAAA+GGG 0.481732 1.2:+73150825 MS.gene54795:CDS
TAGATTTGAAACCTTGCCCT+TGG 0.481800 1.2:-73152098 None:intergenic
TGGTATAACTTTGTTGCCGC+AGG 0.491255 1.2:-73152078 None:intergenic
AGATTGGTGGCTATGTTAGC+AGG 0.496959 1.2:+73150633 MS.gene54795:CDS
CTCAACAAGGGATATGTGGC+TGG 0.532427 1.2:+73150780 MS.gene54795:CDS
GCCAATAACAGCAACCAGAC+AGG 0.535502 1.2:-73150685 None:intergenic
GAATCACAAGCCAGAAGTTG+GGG 0.540089 1.2:+73152018 MS.gene54795:CDS
AAGCCTCAACAAGGGATATG+TGG 0.555870 1.2:+73150776 MS.gene54795:CDS
AGGAATAGCTAAACAATTGG+TGG 0.557067 1.2:+73151987 MS.gene54795:CDS
TGGTTGCAAAAGAACCTGTC+TGG 0.566369 1.2:+73150671 MS.gene54795:CDS
AAGTGAAGACTTCAAGATTG+CGG 0.578803 1.2:+73150737 MS.gene54795:CDS
GTTGAGGATGGATAGATTGG+TGG 0.579094 1.2:+73150620 None:intergenic
AGGGGCCTCTGCGACAGAGA+AGG 0.581862 1.2:+73150844 MS.gene54795:CDS
TGAGGCCAGTTAGCTCCTCC+CGG 0.584487 1.2:-73152129 None:intergenic
CAGCCACATATCCCTTGTTG+AGG 0.606889 1.2:-73150779 None:intergenic
ACAAAGTTATACCAAGGGCA+AGG 0.614543 1.2:+73152087 MS.gene54795:CDS
TGGGAAATTAAGCCTCAACA+AGG 0.631474 1.2:+73150767 MS.gene54795:CDS
TAAATCGCAGTGTAATGCAA+TGG 0.632285 1.2:-73152049 None:intergenic
CAGACTTTCTACCTAGAAAG+GGG 0.636163 1.2:+73150826 MS.gene54795:CDS
AATTGCATACATATCAAATG+TGG 0.643430 1.2:+73151939 MS.gene54795:CDS
CGGCAACAAAGTTATACCAA+GGG 0.644825 1.2:+73152082 MS.gene54795:CDS
GCGGCAACAAAGTTATACCA+AGG 0.651915 1.2:+73152081 MS.gene54795:CDS
ACTCACCTTCTCTGTCGCAG+AGG 0.668717 1.2:-73150849 None:intergenic
GGGAAATTAAGCCTCAACAA+GGG 0.686786 1.2:+73150768 MS.gene54795:CDS
CTGCGATTTACGAAATCCTG+CGG 0.698179 1.2:+73152062 MS.gene54795:CDS
AAATCTATAAAGGTTCCGGG+AGG 0.724022 1.2:+73152114 MS.gene54795:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTAATTTTAACATGTTT+TGG - chr1.2:73151157-73151176 None:intergenic 10.0%
!! TTATGTATAAAAACTATTGT+AGG + chr1.2:73151911-73151930 MS.gene54795:intron 15.0%
!!! ATAAAAAGTAGTTTGTATTT+TGG - chr1.2:73151111-73151130 None:intergenic 15.0%
!!! TGTATCTTTTTCTTATAATT+GGG - chr1.2:73151353-73151372 None:intergenic 15.0%
!!! TTGTATCTTTTTCTTATAAT+TGG - chr1.2:73151354-73151373 None:intergenic 15.0%
!! ATCAAAATTATAGCATTAAG+TGG + chr1.2:73151505-73151524 MS.gene54795:intron 20.0%
!!! ATTTAATTTAGAGTCATGTT+TGG + chr1.2:73151474-73151493 MS.gene54795:intron 20.0%
!!! TATGTTTCTAACTATGTATA+AGG + chr1.2:73151814-73151833 MS.gene54795:intron 20.0%
!!! TTTAATTTAGAGTCATGTTT+GGG + chr1.2:73151475-73151494 MS.gene54795:intron 20.0%
! AATTGCATACATATCAAATG+TGG + chr1.2:73151939-73151958 MS.gene54795:CDS 25.0%
! AGAGAAAAATTTAGACAGAA+AGG + chr1.2:73151967-73151986 MS.gene54795:CDS 25.0%
! AGATAACTTGAAAATTACAC+AGG - chr1.2:73151073-73151092 None:intergenic 25.0%
! ATATGGAATAGATGCATTAA+AGG - chr1.2:73151424-73151443 None:intergenic 25.0%
! ATCTATTCCATATAAACAGT+AGG + chr1.2:73151431-73151450 MS.gene54795:intron 25.0%
! ATGCATTAAAGGACAAAATA+AGG - chr1.2:73151413-73151432 None:intergenic 25.0%
! CTTGATCAAACTTTCTTATT+TGG + chr1.2:73150714-73150733 MS.gene54795:CDS 25.0%
! TATAAAAACTATTGTAGGAC+TGG + chr1.2:73151916-73151935 MS.gene54795:intron 25.0%
! TGCATTAAAGGACAAAATAA+GGG - chr1.2:73151412-73151431 None:intergenic 25.0%
! TTTCAAATCTATAAAGGTTC+CGG + chr1.2:73152110-73152129 MS.gene54795:CDS 25.0%
!! AACATAGTTTACCATTTTGA+AGG - chr1.2:73151800-73151819 None:intergenic 25.0%
!! ACATAGTTTACCATTTTGAA+GGG - chr1.2:73151799-73151818 None:intergenic 25.0%
!! ATCTTTTTCTTATAATTGGG+AGG - chr1.2:73151350-73151369 None:intergenic 25.0%
!!! ACAAACTACTTTTTATCTAG+AGG + chr1.2:73151115-73151134 MS.gene54795:intron 25.0%
!!! ATTGTTTTTTTCTTTGACCA+GGG + chr1.2:73151544-73151563 MS.gene54795:intron 25.0%
!!! TAAAACTTGTTTCTGAATCT+GGG + chr1.2:73150872-73150891 MS.gene54795:intron 25.0%
!!! TTAAAACTTGTTTCTGAATC+TGG + chr1.2:73150871-73150890 MS.gene54795:intron 25.0%
AAAAATTGTTGAGACATGTC+TGG - chr1.2:73151211-73151230 None:intergenic 30.0%
ACTAGTAACATGAGTAAACT+AGG - chr1.2:73151865-73151884 None:intergenic 30.0%
GAAAATTACACAGGAATGAA+AGG - chr1.2:73151064-73151083 None:intergenic 30.0%
GAAAGGAATAGCTAAACAAT+TGG + chr1.2:73151984-73152003 MS.gene54795:CDS 30.0%
GCAAGGTTTCAAATCTATAA+AGG + chr1.2:73152104-73152123 MS.gene54795:CDS 30.0%
TCTAAATTAAATGCTCTTCG+AGG - chr1.2:73151466-73151485 None:intergenic 30.0%
TTCAAATCTATAAAGGTTCC+GGG + chr1.2:73152111-73152130 MS.gene54795:CDS 30.0%
!! TTCTGTTTTAAACAAGTGCA+AGG - chr1.2:73151677-73151696 None:intergenic 30.0%
!!! GATTGTTTTTTTCTTTGACC+AGG + chr1.2:73151543-73151562 MS.gene54795:intron 30.0%
AAGTGAAGACTTCAAGATTG+CGG + chr1.2:73150737-73150756 MS.gene54795:CDS 35.0%
AATTAAATGCTCTTCGAGGA+TGG - chr1.2:73151462-73151481 None:intergenic 35.0%
AGGAATAGCTAAACAATTGG+TGG + chr1.2:73151987-73152006 MS.gene54795:CDS 35.0%
ATGAAGTTGAACTTCACATC+TGG - chr1.2:73152168-73152187 None:intergenic 35.0%
ATGTGATAAATGTTACTCCC+TGG - chr1.2:73151564-73151583 None:intergenic 35.0%
CCAGTCTCATGTATAAGAAA+AGG + chr1.2:73151310-73151329 MS.gene54795:intron 35.0%
GGTAACACCTACTGTTTATA+TGG - chr1.2:73151441-73151460 None:intergenic 35.0%
TAAATCGCAGTGTAATGCAA+TGG - chr1.2:73152052-73152071 None:intergenic 35.0%
TCTCTTCATATTGATCACTG+AGG + chr1.2:73151704-73151723 MS.gene54795:intron 35.0%
TTCTTATACATGAGACTGGA+GGG - chr1.2:73151309-73151328 None:intergenic 35.0%
TTTCTTATACATGAGACTGG+AGG - chr1.2:73151310-73151329 None:intergenic 35.0%
! CCTTTTCTTATACATGAGAC+TGG - chr1.2:73151313-73151332 None:intergenic 35.0%
! TTCACATCTGGAGAAGTTTT+TGG - chr1.2:73152156-73152175 None:intergenic 35.0%
!! AGACAAGCTAGAAGCATTTA+AGG - chr1.2:73151265-73151284 None:intergenic 35.0%
AAATCTATAAAGGTTCCGGG+AGG + chr1.2:73152114-73152133 MS.gene54795:CDS 40.0%
ACAAAGTTATACCAAGGGCA+AGG + chr1.2:73152087-73152106 MS.gene54795:CDS 40.0%
AGAATCACAAGCCAGAAGTT+GGG + chr1.2:73152017-73152036 MS.gene54795:CDS 40.0%
AGTGAAGACTTCAAGATTGC+GGG + chr1.2:73150738-73150757 MS.gene54795:CDS 40.0%
ATTGATCACTGAGGCAAGAA+TGG + chr1.2:73151713-73151732 MS.gene54795:intron 40.0%
CAGACTTTCTACCTAGAAAG+GGG + chr1.2:73150826-73150845 MS.gene54795:CDS 40.0%
CGGCAACAAAGTTATACCAA+GGG + chr1.2:73152082-73152101 MS.gene54795:CDS 40.0%
GCAGACTTTCTACCTAGAAA+GGG + chr1.2:73150825-73150844 MS.gene54795:CDS 40.0%
GCAGGATTCTCTTTACCAAA+TGG + chr1.2:73150651-73150670 MS.gene54795:CDS 40.0%
GGGAAATTAAGCCTCAACAA+GGG + chr1.2:73150768-73150787 MS.gene54795:CDS 40.0%
TCCTGCATTACCCTTCAAAA+TGG + chr1.2:73151786-73151805 MS.gene54795:intron 40.0%
TGACATGCGATGAAAGAACA+TGG - chr1.2:73151629-73151648 None:intergenic 40.0%
TGCAGACTTTCTACCTAGAA+AGG + chr1.2:73150824-73150843 MS.gene54795:CDS 40.0%
TGGGAAATTAAGCCTCAACA+AGG + chr1.2:73150767-73150786 MS.gene54795:CDS 40.0%
! AGCTAAACAATTGGTGGCTA+AGG + chr1.2:73151993-73152012 MS.gene54795:CDS 40.0%
! CAGGTTCTTTTGCAACCATT+TGG - chr1.2:73150669-73150688 None:intergenic 40.0%
! TAGATTTGAAACCTTGCCCT+TGG - chr1.2:73152101-73152120 None:intergenic 40.0%
!! ACCATTTTGAAGGGTAATGC+AGG - chr1.2:73151790-73151809 None:intergenic 40.0%
!! CTGAGGCAAGAATGGATTTT+TGG + chr1.2:73151721-73151740 MS.gene54795:intron 40.0%
!! TCAAGATTGCGGGTTTTGAT+GGG + chr1.2:73150748-73150767 MS.gene54795:CDS 40.0%
!! TTCAAGATTGCGGGTTTTGA+TGG + chr1.2:73150747-73150766 MS.gene54795:CDS 40.0%
!!! TCTGGAGAAGTTTTTGGTTG+AGG - chr1.2:73152150-73152169 None:intergenic 40.0%
AAGCCTCAACAAGGGATATG+TGG + chr1.2:73150776-73150795 MS.gene54795:CDS 45.0%
ACACAGGAATGAAAGGCAAG+CGG - chr1.2:73151057-73151076 None:intergenic 45.0%
CAGAATCACAAGCCAGAAGT+TGG + chr1.2:73152016-73152035 MS.gene54795:CDS 45.0%
CGCATGTCAATAGTTCTTCG+TGG + chr1.2:73151640-73151659 MS.gene54795:intron 45.0%
CTGAATTCGCTCTTGTCTTC+CGG + chr1.2:73151182-73151201 MS.gene54795:intron 45.0%
CTGCGATTTACGAAATCCTG+CGG + chr1.2:73152062-73152081 MS.gene54795:CDS 45.0%
GAATCACAAGCCAGAAGTTG+GGG + chr1.2:73152018-73152037 MS.gene54795:CDS 45.0%
GCGGCAACAAAGTTATACCA+AGG + chr1.2:73152081-73152100 MS.gene54795:CDS 45.0%
TGAATCTGGGTTTGTGAGTG+AGG + chr1.2:73150885-73150904 MS.gene54795:intron 45.0%
TGGTATAACTTTGTTGCCGC+AGG - chr1.2:73152081-73152100 None:intergenic 45.0%
TGGTTGCAAAAGAACCTGTC+TGG + chr1.2:73150671-73150690 MS.gene54795:CDS 45.0%
! ACCTGTCTGGTTGCTGTTAT+TGG + chr1.2:73150684-73150703 MS.gene54795:CDS 45.0%
! AGATTGGTGGCTATGTTAGC+AGG + chr1.2:73150633-73150652 MS.gene54795:CDS 45.0%
CAGCCACATATCCCTTGTTG+AGG - chr1.2:73150782-73150801 None:intergenic 50.0%
CTCAACAAGGGATATGTGGC+TGG + chr1.2:73150780-73150799 MS.gene54795:CDS 50.0%
GCCAATAACAGCAACCAGAC+AGG - chr1.2:73150688-73150707 None:intergenic 50.0%
GTTGAGACATGTCTGGACTC+CGG - chr1.2:73151204-73151223 None:intergenic 50.0%
! ATGAGACTGGAGGGAGTATG+TGG - chr1.2:73151300-73151319 None:intergenic 50.0%
ACTCACCTTCTCTGTCGCAG+AGG - chr1.2:73150852-73150871 None:intergenic 55.0%
AGGTTCCGGGAGGAGCTAAC+TGG + chr1.2:73152124-73152143 MS.gene54795:CDS 60.0%
GGCACGACATCCCCAACTTC+TGG - chr1.2:73152031-73152050 None:intergenic 60.0%
TGAGGCCAGTTAGCTCCTCC+CGG - chr1.2:73152132-73152151 None:intergenic 60.0%
TGTCGCAGAGGCCCCTTTCT+AGG - chr1.2:73150840-73150859 None:intergenic 60.0%
AGGGGCCTCTGCGACAGAGA+AGG + chr1.2:73150844-73150863 MS.gene54795:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 73150627 73152221 73150627 ID=MS.gene54795
chr1.2 mRNA 73150627 73152221 73150627 ID=MS.gene54795.t1;Parent=MS.gene54795
chr1.2 exon 73150627 73150865 73150627 ID=MS.gene54795.t1.exon1;Parent=MS.gene54795.t1
chr1.2 CDS 73150627 73150865 73150627 ID=cds.MS.gene54795.t1;Parent=MS.gene54795.t1
chr1.2 exon 73151933 73152221 73151933 ID=MS.gene54795.t1.exon2;Parent=MS.gene54795.t1
chr1.2 CDS 73151933 73152221 73151933 ID=cds.MS.gene54795.t1;Parent=MS.gene54795.t1
Gene Sequence

>MS.gene54795

ATGGATAGATTGGTGGCTATGTTAGCAGGATTCTCTTTACCAAATGGTTGCAAAAGAACCTGTCTGGTTGCTGTTATTGGCAATTCACTTGATCAAACTTTCTTATTTGGAAGTGAAGACTTCAAGATTGCGGGTTTTGATGGGAAATTAAGCCTCAACAAGGGATATGTGGCTGGCATATTAACAGTAGATACTGTTGCAGACTTTCTACCTAGAAAGGGGCCTCTGCGACAGAGAAGGTGAGTTAAAACTTGTTTCTGAATCTGGGTTTGTGAGTGAGGATTTATGACACGAATAATGATTGATATGCAAAAACTGTGTATGAATATACTTGAGACTAAAAATCATCTTTGAACGCAAAAACTACAATTTCTTGATACAAGTTAAAACTGTGTACGAATAAACTTGAGACTAATAAACTTGTTTTCCGCTTGCCTTTCATTCCTGTGTAATTTTCAAGTTATCTGTATTTGAAAATTTACCAAAATACAAACTACTTTTTATCTAGAGGCAAAATCAATTGTACTCCAAAACATGTTAAAATTAATTTTGTGTCTGAATTCGCTCTTGTCTTCCGGAGTCCAGACATGTCTCAACAATTTTTAGATAATTATGTTCTGTTATAAACATATAGACCTTAAATGCTTCTAGCTTGTCTTGTTTTAACTATCCACATACTCCCTCCAGTCTCATGTATAAGAAAAGGATAGAAGTTTTTTTCCTCCCAATTATAAGAAAAAGATACAATTTAAGATTTATTAATTAATTATTTTACTTTTATACCCTTATTTTGTCCTTTAATGCATCTATTCCATATAAACAGTAGGTGTTACCATCCTCGAAGAGCATTTAATTTAGAGTCATGTTTGGGATAAACTATCAAAATTATAGCATTAAGTGGTCTCATCTTCTTGATGATTGTTTTTTTCTTTGACCAGGGAGTAACATTTATCACATAACTTGTCTTTCATTTGTGTTTTGCTGTAAAAGTAACTAAGTCCATGTTCTTTCATCGCATGTCAATAGTTCTTCGTGGTTAGTTTGTAGCCTTGCACTTGTTTAAAACAGAAGTTAAGATCTCTTCATATTGATCACTGAGGCAAGAATGGATTTTTGGTTAATCGAAACATGCTAAAAATCTTTTATGAAATTCATTGATTCCTGCATTACCCTTCAAAATGGTAAACTATGTTTCTAACTATGTATAAGGCGATGTTAGATTATGTATTTATGTTCCTAGTTTACTCATGTTACTAGTGAATCTTTGCTTATAGCTGCAACTTTTTATGTATAAAAACTATTGTAGGACTGGAATTGCATACATATCAAATGTGGCAGTGAGAGAAAAATTTAGACAGAAAGGAATAGCTAAACAATTGGTGGCTAAGGCAGAATCACAAGCCAGAAGTTGGGGATGTCGTGCCATTGCATTACACTGCGATTTACGAAATCCTGCGGCAACAAAGTTATACCAAGGGCAAGGTTTCAAATCTATAAAGGTTCCGGGAGGAGCTAACTGGCCTCAACCAAAAACTTCTCCAGATGTGAAGTTCAACTTCATGATGAAGCTTCTCAACAAATCAGCAGTTTCTTAA

Protein sequence

>MS.gene54795.t1

MDRLVAMLAGFSLPNGCKRTCLVAVIGNSLDQTFLFGSEDFKIAGFDGKLSLNKGYVAGILTVDTVADFLPRKGPLRQRRTGIAYISNVAVREKFRQKGIAKQLVAKAESQARSWGCRAIALHCDLRNPAATKLYQGQGFKSIKVPGGANWPQPKTSPDVKFNFMMKLLNKSAVS