Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063076.t1 | XP_003604084.1 | 95.9 | 314 | 12 | 1 | 1 | 313 | 1 | 314 | 2.00E-176 | 628.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063076.t1 | G7J991 | 95.9 | 314 | 12 | 1 | 1 | 313 | 1 | 314 | 1.4e-176 | 628.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene063076 | 0.852329 | 4.96E-61 | -1.69E-46 |
| MS.gene049173 | MS.gene063076 | 0.804419 | 2.18E-49 | -1.69E-46 |
| MS.gene049222 | MS.gene063076 | 0.834866 | 2.34E-56 | -1.69E-46 |
| MS.gene049232 | MS.gene063076 | 0.820981 | 5.15E-53 | -1.69E-46 |
| MS.gene049285 | MS.gene063076 | 0.859838 | 3.14E-63 | -1.69E-46 |
| MS.gene049320 | MS.gene063076 | 0.866202 | 3.39E-65 | -1.69E-46 |
| MS.gene04939 | MS.gene063076 | 0.81507 | 1.12E-51 | -1.69E-46 |
| MS.gene049633 | MS.gene063076 | 0.80044 | 1.44E-48 | -1.69E-46 |
| MS.gene05010 | MS.gene063076 | 0.83875 | 2.39E-57 | -1.69E-46 |
| MS.gene050163 | MS.gene063076 | 0.855059 | 8.13E-62 | -1.69E-46 |
| MS.gene050165 | MS.gene063076 | 0.86006 | 2.69E-63 | -1.69E-46 |
| MS.gene050170 | MS.gene063076 | 0.811518 | 6.72E-51 | -1.69E-46 |
| MS.gene050323 | MS.gene063076 | 0.816093 | 6.60E-52 | -1.69E-46 |
| MS.gene050333 | MS.gene063076 | 0.826729 | 2.31E-54 | -1.69E-46 |
| MS.gene050496 | MS.gene063076 | 0.823297 | 1.49E-53 | -1.69E-46 |
| MS.gene050504 | MS.gene063076 | 0.871195 | 8.22E-67 | -1.69E-46 |
| MS.gene050518 | MS.gene063076 | 0.85246 | 4.55E-61 | -1.69E-46 |
| MS.gene050577 | MS.gene063076 | 0.847567 | 1.07E-59 | -1.69E-46 |
| MS.gene050612 | MS.gene063076 | 0.888154 | 7.59E-73 | -1.69E-46 |
| MS.gene050675 | MS.gene063076 | 0.819899 | 9.11E-53 | -1.69E-46 |
| MS.gene050699 | MS.gene063076 | 0.848979 | 4.34E-60 | -1.69E-46 |
| MS.gene050772 | MS.gene063076 | 0.813412 | 2.59E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene063076 | 0.802892 | 4.52E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene063076 | 0.802489 | 5.47E-49 | -1.69E-46 |
| MS.gene050876 | MS.gene063076 | 0.836232 | 1.06E-56 | -1.69E-46 |
| MS.gene050904 | MS.gene063076 | 0.808563 | 2.91E-50 | -1.69E-46 |
| MS.gene050986 | MS.gene063076 | 0.87228 | 3.59E-67 | -1.69E-46 |
| MS.gene05100 | MS.gene063076 | 0.848986 | 4.32E-60 | -1.69E-46 |
| MS.gene051317 | MS.gene063076 | 0.813109 | 3.02E-51 | -1.69E-46 |
| MS.gene051551 | MS.gene063076 | 0.830977 | 2.17E-55 | -1.69E-46 |
| MS.gene051810 | MS.gene063076 | 0.819306 | 1.24E-52 | -1.69E-46 |
| MS.gene052164 | MS.gene063076 | 0.84471 | 6.39E-59 | -1.69E-46 |
| MS.gene052482 | MS.gene063076 | 0.842166 | 3.05E-58 | -1.69E-46 |
| MS.gene052486 | MS.gene063076 | 0.844241 | 8.54E-59 | -1.69E-46 |
| MS.gene052966 | MS.gene063076 | 0.803261 | 3.79E-49 | -1.69E-46 |
| MS.gene053203 | MS.gene063076 | 0.812195 | 4.79E-51 | -1.69E-46 |
| MS.gene053213 | MS.gene063076 | 0.826836 | 2.18E-54 | -1.69E-46 |
| MS.gene053286 | MS.gene063076 | 0.832385 | 9.75E-56 | -1.69E-46 |
| MS.gene053436 | MS.gene063076 | 0.802337 | 5.89E-49 | -1.69E-46 |
| MS.gene053574 | MS.gene063076 | 0.814453 | 1.53E-51 | -1.69E-46 |
| MS.gene054408 | MS.gene063076 | 0.808483 | 3.03E-50 | -1.69E-46 |
| MS.gene054595 | MS.gene063076 | 0.819455 | 1.15E-52 | -1.69E-46 |
| MS.gene054600 | MS.gene063076 | 0.825428 | 4.71E-54 | -1.69E-46 |
| MS.gene054642 | MS.gene063076 | 0.838217 | 3.28E-57 | -1.69E-46 |
| MS.gene05470 | MS.gene063076 | 0.85945 | 4.10E-63 | -1.69E-46 |
| MS.gene054954 | MS.gene063076 | 0.884223 | 2.30E-71 | -1.69E-46 |
| MS.gene055362 | MS.gene063076 | 0.867378 | 1.43E-65 | -1.69E-46 |
| MS.gene055502 | MS.gene063076 | 0.839147 | 1.88E-57 | -1.69E-46 |
| MS.gene055748 | MS.gene063076 | 0.855246 | 7.18E-62 | -1.69E-46 |
| MS.gene055750 | MS.gene063076 | 0.848028 | 7.96E-60 | -1.69E-46 |
| MS.gene055751 | MS.gene063076 | 0.863165 | 3.02E-64 | -1.69E-46 |
| MS.gene055776 | MS.gene063076 | 0.874605 | 5.93E-68 | -1.69E-46 |
| MS.gene055849 | MS.gene063076 | 0.841732 | 3.98E-58 | -1.69E-46 |
| MS.gene05589 | MS.gene063076 | 0.861466 | 1.01E-63 | -1.69E-46 |
| MS.gene056374 | MS.gene063076 | 0.805351 | 1.39E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene063076 | 0.872049 | 4.28E-67 | -1.69E-46 |
| MS.gene056510 | MS.gene063076 | 0.869699 | 2.55E-66 | -1.69E-46 |
| MS.gene056657 | MS.gene063076 | 0.862847 | 3.79E-64 | -1.69E-46 |
| MS.gene057831 | MS.gene063076 | 0.840847 | 6.79E-58 | -1.69E-46 |
| MS.gene05787 | MS.gene063076 | 0.802967 | 4.36E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene063076 | 0.801026 | 1.09E-48 | -1.69E-46 |
| MS.gene058419 | MS.gene063076 | 0.851927 | 6.45E-61 | -1.69E-46 |
| MS.gene058544 | MS.gene063076 | 0.824163 | 9.37E-54 | -1.69E-46 |
| MS.gene058719 | MS.gene063076 | 0.834582 | 2.76E-56 | -1.69E-46 |
| MS.gene058773 | MS.gene063076 | 0.8339 | 4.09E-56 | -1.69E-46 |
| MS.gene059022 | MS.gene063076 | 0.878593 | 2.48E-69 | -1.69E-46 |
| MS.gene059023 | MS.gene063076 | 0.813371 | 2.65E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene063076 | 0.86249 | 4.88E-64 | -1.69E-46 |
| MS.gene059718 | MS.gene063076 | 0.826871 | 2.14E-54 | -1.69E-46 |
| MS.gene059837 | MS.gene063076 | 0.82223 | 2.65E-53 | -1.69E-46 |
| MS.gene059865 | MS.gene063076 | 0.876787 | 1.06E-68 | -1.69E-46 |
| MS.gene06000 | MS.gene063076 | 0.809705 | 1.66E-50 | -1.69E-46 |
| MS.gene060526 | MS.gene063076 | 0.801357 | 9.36E-49 | -1.69E-46 |
| MS.gene060703 | MS.gene063076 | 0.858372 | 8.62E-63 | -1.69E-46 |
| MS.gene060762 | MS.gene063076 | 0.830856 | 2.32E-55 | -1.69E-46 |
| MS.gene060778 | MS.gene063076 | 0.835819 | 1.34E-56 | -1.69E-46 |
| MS.gene06077 | MS.gene063076 | 0.872664 | 2.67E-67 | -1.69E-46 |
| MS.gene060982 | MS.gene063076 | 0.865741 | 4.74E-65 | -1.69E-46 |
| MS.gene061150 | MS.gene063076 | 0.805134 | 1.55E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063076.t1 | MTR_3g118320 | 97.452 | 314 | 7 | 1 | 1 | 313 | 1 | 314 | 0.0 | 626 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063076.t1 | AT5G17670 | 68.070 | 285 | 84 | 4 | 30 | 313 | 31 | 309 | 8.67e-137 | 390 |
Find 115 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTGTATATGCAGCAATTT+GGG | 0.178912 | 3.3:-95938760 | MS.gene063076:CDS |
| CGTCTGTATATGCAGCAATT+TGG | 0.240390 | 3.3:-95938761 | MS.gene063076:CDS |
| AAGAGTCCCACGCCAGTAAT+TGG | 0.246638 | 3.3:+95938910 | None:intergenic |
| CATTCACCTGCTTGTATCCT+TGG | 0.277029 | 3.3:+95938255 | None:intergenic |
| ATAACACCAGGGACGCCTTT+TGG | 0.317246 | 3.3:+95938686 | None:intergenic |
| TCAGAGTTGCCTCCAGCTTC+TGG | 0.320028 | 3.3:+95939014 | None:intergenic |
| TTGGATTTGAGTTTCCAAAA+AGG | 0.331624 | 3.3:+95938393 | None:intergenic |
| TATTTCTTGAACCAGGGGTT+AGG | 0.343354 | 3.3:-95939055 | MS.gene063076:intron |
| ACATTGCGTGCTCGCTTTGT+TGG | 0.348023 | 3.3:-95938278 | MS.gene063076:CDS |
| AGTGAGCATTTGGATGGAAT+TGG | 0.358570 | 3.3:+95939200 | None:intergenic |
| CGATCAAACCAGCGGCATTT+CGG | 0.365710 | 3.3:+95938936 | None:intergenic |
| AGAAGAAGACAGTGAGCATT+TGG | 0.366919 | 3.3:+95939190 | None:intergenic |
| GGAAGTGCAGGCTCAGCATT+TGG | 0.368790 | 3.3:+95938374 | None:intergenic |
| ATATCATTGTTATTAACTCT+TGG | 0.371502 | 3.3:-95938728 | MS.gene063076:CDS |
| GTTTGATCGATCCCAATTAC+TGG | 0.372683 | 3.3:-95938922 | MS.gene063076:CDS |
| AGTTAATAACAATGATATAT+TGG | 0.374538 | 3.3:+95938733 | None:intergenic |
| TTTGTGGGCAAGCAGATGTA+TGG | 0.392192 | 3.3:-95938111 | MS.gene063076:CDS |
| AACCAACGCCTGTCCTTGAT+TGG | 0.395983 | 3.3:-95938886 | MS.gene063076:CDS |
| ATCCATTGTTCCAACACATC+AGG | 0.397261 | 3.3:+95937968 | None:intergenic |
| TAATAATTTCTCAGGTTTGT+GGG | 0.407374 | 3.3:-95938126 | MS.gene063076:intron |
| CAGTCTCCTCGGGAAGTAGC+TGG | 0.414848 | 3.3:+95938339 | None:intergenic |
| CGAGCACGCAATGTTATGTT+AGG | 0.415750 | 3.3:+95938287 | None:intergenic |
| TGTTAACTTGTGGAGCAAAA+TGG | 0.418912 | 3.3:-95939235 | MS.gene063076:CDS |
| TTGAGGTGAGGGGTGTAAAC+AGG | 0.422172 | 3.3:+95938614 | None:intergenic |
| TCAAGATGAGCTGATACTTC+TGG | 0.423594 | 3.3:+95938073 | None:intergenic |
| CGGTGCACTTAACATTTCCC+TGG | 0.441926 | 3.3:-95938050 | MS.gene063076:CDS |
| GAAGTATCAGCTCATCTTGA+CGG | 0.443659 | 3.3:-95938070 | MS.gene063076:CDS |
| CAGGGGTTAGGGAACAATTC+CGG | 0.447536 | 3.3:-95939043 | MS.gene063076:CDS |
| AGAGTCCCACGCCAGTAATT+GGG | 0.459641 | 3.3:+95938911 | None:intergenic |
| CTCAAGTATGTATGCATAGC+AGG | 0.460049 | 3.3:-95938596 | MS.gene063076:CDS |
| ATGACATCATCTGAACCAAC+CGG | 0.461617 | 3.3:+95938010 | None:intergenic |
| ATTTCTTGAACCAGGGGTTA+GGG | 0.465909 | 3.3:-95939054 | MS.gene063076:intron |
| CCACCTCCAAAAGGCGTCCC+TGG | 0.469313 | 3.3:-95938692 | MS.gene063076:CDS |
| GCGGCATTTCGGAGCCAATC+AGG | 0.471608 | 3.3:+95938947 | None:intergenic |
| ACAAAATGAAGGTATATTCA+AGG | 0.471722 | 3.3:-95938422 | MS.gene063076:intron |
| CTCTCTTCAAATACCAATCA+AGG | 0.471850 | 3.3:+95938873 | None:intergenic |
| CGTGCTCGCTTTGTTGGCCA+AGG | 0.474939 | 3.3:-95938272 | MS.gene063076:CDS |
| ATAATAATTTCTCAGGTTTG+TGG | 0.487916 | 3.3:-95938127 | MS.gene063076:intron |
| GAGACCACTCTATCATTGAT+TGG | 0.491581 | 3.3:-95938809 | MS.gene063076:CDS |
| CATCTTCAAGCTAATTAAAG+AGG | 0.492324 | 3.3:+95937939 | None:intergenic |
| TCATTGATTGGTCACTCGGC+TGG | 0.494886 | 3.3:-95938797 | MS.gene063076:CDS |
| GATTGGCTCCGAAATGCCGC+TGG | 0.497694 | 3.3:-95938944 | MS.gene063076:CDS |
| GAAGTATCTGTAGGAAGTGC+AGG | 0.505483 | 3.3:+95938362 | None:intergenic |
| GGGAGTGGTAAACTCCATCC+AGG | 0.507069 | 3.3:+95938032 | None:intergenic |
| CTCAGGAGGTGCTAACTCCA+TGG | 0.507697 | 3.3:+95938829 | None:intergenic |
| TTGATAATAGCAGTCTCCTC+GGG | 0.515621 | 3.3:+95938329 | None:intergenic |
| GCACTTAACATTTCCCTGGA+TGG | 0.518025 | 3.3:-95938046 | MS.gene063076:CDS |
| TTGGTCACTCGGCTGGAGGA+TGG | 0.518671 | 3.3:-95938790 | MS.gene063076:CDS |
| CTGGTGTTATCGATCAAACC+AGG | 0.522198 | 3.3:-95938673 | MS.gene063076:CDS |
| AATATTGTTGAACATAATCA+AGG | 0.524897 | 3.3:+95938645 | None:intergenic |
| CCAATCAGGTCTTGACACCT+TGG | 0.525622 | 3.3:+95938961 | None:intergenic |
| CAAAATGAAGGTATATTCAA+GGG | 0.525750 | 3.3:-95938421 | MS.gene063076:intron |
| ATCAATGATAGAGTGGTCTC+AGG | 0.527336 | 3.3:+95938812 | None:intergenic |
| CCCCTGATGTGTTGGAACAA+TGG | 0.527934 | 3.3:-95937970 | MS.gene063076:CDS |
| TTGTGGGCAAGCAGATGTAT+GGG | 0.530004 | 3.3:-95938110 | MS.gene063076:CDS |
| TCTATCATTGATTGGTCACT+CGG | 0.533151 | 3.3:-95938801 | MS.gene063076:CDS |
| TTCAAATACCAATCAAGGAC+AGG | 0.534115 | 3.3:+95938878 | None:intergenic |
| ATCGATCCCAATTACTGGCG+TGG | 0.534397 | 3.3:-95938917 | MS.gene063076:CDS |
| CCTCATCATCCACCTCCAAA+AGG | 0.539353 | 3.3:-95938701 | MS.gene063076:CDS |
| TCCATTGTTCCAACACATCA+GGG | 0.540776 | 3.3:+95937969 | None:intergenic |
| TTCCGGTGACTACCAGAAGC+TGG | 0.544893 | 3.3:-95939026 | MS.gene063076:CDS |
| TGCATACATACTTGAGGTGA+GGG | 0.545124 | 3.3:+95938603 | None:intergenic |
| GTATGGTTCCCCTGATGTGT+TGG | 0.547140 | 3.3:-95937978 | MS.gene063076:CDS |
| CAAGCAGATGTATGGGGCGA+TGG | 0.548229 | 3.3:-95938103 | MS.gene063076:CDS |
| TTTGATAATAGCAGTCTCCT+CGG | 0.548689 | 3.3:+95938328 | None:intergenic |
| CTGGTTTGATCGATAACACC+AGG | 0.550685 | 3.3:+95938674 | None:intergenic |
| CTCCAGCTTCTGGTAGTCAC+CGG | 0.551919 | 3.3:+95939024 | None:intergenic |
| CCAAGGTGTCAAGACCTGAT+TGG | 0.553014 | 3.3:-95938961 | MS.gene063076:CDS |
| GAAGACAGTGAGCATTTGGA+TGG | 0.553333 | 3.3:+95939194 | None:intergenic |
| GGATGGAGTTTACCACTCCC+CGG | 0.562006 | 3.3:-95938029 | MS.gene063076:CDS |
| GACGGCGGGTCGATAGTTGT+TGG | 0.564129 | 3.3:+95939154 | None:intergenic |
| GAGACTAACCGGGAGAATGA+CGG | 0.567802 | 3.3:+95939136 | None:intergenic |
| TCGACCCGCCGTCATTCTCC+CGG | 0.569276 | 3.3:-95939144 | MS.gene063076:intron |
| AGTCTCCTCGGGAAGTAGCT+GGG | 0.569738 | 3.3:+95938340 | None:intergenic |
| AACATAATCAAGGAGACCCC+TGG | 0.569925 | 3.3:+95938655 | None:intergenic |
| CATCTGAACCAACCGGGGAG+TGG | 0.574033 | 3.3:+95938017 | None:intergenic |
| AGTTGATGATGCTGTTCAAG+AGG | 0.575707 | 3.3:-95938852 | MS.gene063076:CDS |
| CGTGTCCACAGTTGTTGCCA+AGG | 0.576292 | 3.3:-95938978 | MS.gene063076:CDS |
| GGCGGGTCGATAGTTGTTGG+TGG | 0.583420 | 3.3:+95939157 | None:intergenic |
| GGAGTTTACCACTCCCCGGT+TGG | 0.585654 | 3.3:-95938025 | MS.gene063076:CDS |
| TGACATCATCTGAACCAACC+GGG | 0.591852 | 3.3:+95938011 | None:intergenic |
| TGATGCTGTTCAAGAGGCCA+TGG | 0.593740 | 3.3:-95938846 | MS.gene063076:CDS |
| TGTTGGCCAAGGATACAAGC+AGG | 0.599233 | 3.3:-95938261 | MS.gene063076:intron |
| GATGTCATTAGACCATGGTA+TGG | 0.601444 | 3.3:-95937995 | MS.gene063076:CDS |
| TACCAATCAAGGACAGGCGT+TGG | 0.602600 | 3.3:+95938884 | None:intergenic |
| CGAGCAGTGATGTTAACTTG+TGG | 0.605981 | 3.3:-95939245 | None:intergenic |
| TTGATTGGTCACTCGGCTGG+AGG | 0.606373 | 3.3:-95938794 | MS.gene063076:CDS |
| TGACACCTTGGCAACAACTG+TGG | 0.611400 | 3.3:+95938973 | None:intergenic |
| CTGCTATGCATACATACTTG+AGG | 0.615240 | 3.3:+95938597 | None:intergenic |
| ATGCATACATACTTGAGGTG+AGG | 0.617128 | 3.3:+95938602 | None:intergenic |
| TGTTATGTTAGGTGAAGAAG+TGG | 0.622081 | 3.3:+95938298 | None:intergenic |
| AAAATGAAGGTATATTCAAG+GGG | 0.625084 | 3.3:-95938420 | MS.gene063076:intron |
| TCGATCCCAATTACTGGCGT+GGG | 0.628181 | 3.3:-95938916 | MS.gene063076:CDS |
| TACTTCCCAGCTACTTCCCG+AGG | 0.636324 | 3.3:-95938345 | MS.gene063076:CDS |
| AGTAGCTGGGAAGTATCTGT+AGG | 0.636586 | 3.3:+95938353 | None:intergenic |
| GCAACTCTGAACAATAACTA+CGG | 0.639683 | 3.3:-95939001 | MS.gene063076:CDS |
| GTGACCAATCAATGATAGAG+TGG | 0.646895 | 3.3:+95938805 | None:intergenic |
| AATGATAGAGTGGTCTCAGG+AGG | 0.654021 | 3.3:+95938815 | None:intergenic |
| TGTGGGCAAGCAGATGTATG+GGG | 0.663496 | 3.3:-95938109 | MS.gene063076:CDS |
| AGTATGTATGCATAGCAGGA+AGG | 0.664109 | 3.3:-95938592 | MS.gene063076:intron |
| TGGTGTTATCGATCAAACCA+GGG | 0.665729 | 3.3:-95938672 | MS.gene063076:CDS |
| CAGATGATGTCATTAGACCA+TGG | 0.669037 | 3.3:-95938000 | MS.gene063076:CDS |
| GACATCATCTGAACCAACCG+GGG | 0.670989 | 3.3:+95938012 | None:intergenic |
| GGAGTGGTAAACTCCATCCA+GGG | 0.679305 | 3.3:+95938033 | None:intergenic |
| TCTGTATATGCAGCAATTTG+GGG | 0.680712 | 3.3:-95938759 | MS.gene063076:CDS |
| GCATTTGGATGGAATTGGAG+AGG | 0.682203 | 3.3:+95939205 | None:intergenic |
| CCATTGTTCCAACACATCAG+GGG | 0.693490 | 3.3:+95937970 | None:intergenic |
| GCATACATACTTGAGGTGAG+GGG | 0.697105 | 3.3:+95938604 | None:intergenic |
| CTAACCGGGAGAATGACGGC+GGG | 0.709087 | 3.3:+95939140 | None:intergenic |
| GGTGTTATCGATCAAACCAG+GGG | 0.713378 | 3.3:-95938671 | MS.gene063076:CDS |
| CGGTGACTACCAGAAGCTGG+AGG | 0.713933 | 3.3:-95939023 | MS.gene063076:CDS |
| ACATCAGGGGAACCATACCA+TGG | 0.720174 | 3.3:+95937983 | None:intergenic |
| ATTGGGATCGATCAAACCAG+CGG | 0.724154 | 3.3:+95938928 | None:intergenic |
| ACTAACCGGGAGAATGACGG+CGG | 0.725962 | 3.3:+95939139 | None:intergenic |
| TGGTTTGATCGATAACACCA+GGG | 0.758878 | 3.3:+95938675 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATTATTATTAGAACTT+TGG | + | chr3.3:95939047-95939066 | None:intergenic | 10.0% |
| !! | AGTTAATAACAATGATATAT+TGG | + | chr3.3:95938455-95938474 | None:intergenic | 15.0% |
| !! | TCTAATAATAATAATTTCTC+AGG | - | chr3.3:95939051-95939070 | MS.gene063076:intron | 15.0% |
| !! | AATATTGTTGAACATAATCA+AGG | + | chr3.3:95938543-95938562 | None:intergenic | 20.0% |
| !! | TTAATTTATAGCCAAAACTA+TGG | - | chr3.3:95938972-95938991 | MS.gene063076:CDS | 20.0% |
| !!! | ATATCATTGTTATTAACTCT+TGG | - | chr3.3:95938457-95938476 | MS.gene063076:intron | 20.0% |
| !!! | TAGTTTTGGCTATAAATTAA+CGG | + | chr3.3:95938972-95938991 | None:intergenic | 20.0% |
| ! | ATAATAATTTCTCAGGTTTG+TGG | - | chr3.3:95939058-95939077 | MS.gene063076:intron | 25.0% |
| ! | ATAGTAAGTTATGTTTGTGT+TGG | + | chr3.3:95938714-95938733 | None:intergenic | 25.0% |
| ! | CATTTCTTACTACAAAATGA+AGG | - | chr3.3:95938752-95938771 | MS.gene063076:CDS | 25.0% |
| ! | TAATAATTTCTCAGGTTTGT+GGG | - | chr3.3:95939059-95939078 | MS.gene063076:intron | 25.0% |
| !! | AAAATGAAGGTATATTCAAG+GGG | - | chr3.3:95938765-95938784 | MS.gene063076:CDS | 25.0% |
| !! | ACAAAATGAAGGTATATTCA+AGG | - | chr3.3:95938763-95938782 | MS.gene063076:CDS | 25.0% |
| !! | CAAAATGAAGGTATATTCAA+GGG | - | chr3.3:95938764-95938783 | MS.gene063076:CDS | 25.0% |
| !!! | CAAAAAAAGACTACTTTTGA+GGG | + | chr3.3:95938651-95938670 | None:intergenic | 25.0% |
| !!! | GTTTTTTATTTCTTGAACCA+GGG | - | chr3.3:95938124-95938143 | MS.gene063076:intron | 25.0% |
| !!! | TCAAAAAAAGACTACTTTTG+AGG | + | chr3.3:95938652-95938671 | None:intergenic | 25.0% |
| !!! | TTTTTTATTTCTTGAACCAG+GGG | - | chr3.3:95938125-95938144 | MS.gene063076:intron | 25.0% |
| AAAGAGAAATAGAGACTAAC+CGG | + | chr3.3:95938063-95938082 | None:intergenic | 30.0% | |
| TTATTTGCTTGTGATAGATG+CGG | - | chr3.3:95939009-95939028 | MS.gene063076:CDS | 30.0% | |
| ! | TGGATTTGAGTTTCCAAAAA+GGG | + | chr3.3:95938794-95938813 | None:intergenic | 30.0% |
| ! | TTGGATTTGAGTTTCCAAAA+AGG | + | chr3.3:95938795-95938814 | None:intergenic | 30.0% |
| !!! | GGTTTTTTATTTCTTGAACC+AGG | - | chr3.3:95938123-95938142 | MS.gene063076:intron | 30.0% |
| AAGAGAAATAGAGACTAACC+GGG | + | chr3.3:95938062-95938081 | None:intergenic | 35.0% | |
| CTCTCTTCAAATACCAATCA+AGG | + | chr3.3:95938315-95938334 | None:intergenic | 35.0% | |
| GCAACTCTGAACAATAACTA+CGG | - | chr3.3:95938184-95938203 | MS.gene063076:intron | 35.0% | |
| GTCTGTATATGCAGCAATTT+GGG | - | chr3.3:95938425-95938444 | MS.gene063076:intron | 35.0% | |
| TCTGTATATGCAGCAATTTG+GGG | - | chr3.3:95938426-95938445 | MS.gene063076:intron | 35.0% | |
| TGTTATGTTAGGTGAAGAAG+TGG | + | chr3.3:95938890-95938909 | None:intergenic | 35.0% | |
| TTCAAATACCAATCAAGGAC+AGG | + | chr3.3:95938310-95938329 | None:intergenic | 35.0% | |
| ! | AAGTTATGTTTGTGTTGGCA+TGG | + | chr3.3:95938709-95938728 | None:intergenic | 35.0% |
| ! | GGATTTGAGTTTCCAAAAAG+GGG | + | chr3.3:95938793-95938812 | None:intergenic | 35.0% |
| ! | TCTATCATTGATTGGTCACT+CGG | - | chr3.3:95938384-95938403 | MS.gene063076:CDS | 35.0% |
| ! | TTTGATAATAGCAGTCTCCT+CGG | + | chr3.3:95938860-95938879 | None:intergenic | 35.0% |
| !! | TGTTAACTTGTGGAGCAAAA+TGG | - | chr3.3:95937950-95937969 | MS.gene063076:CDS | 35.0% |
| AACCAAATGAATGCGATGTG+AGG | + | chr3.3:95938107-95938126 | None:intergenic | 40.0% | |
| AGAAGAAGACAGTGAGCATT+TGG | + | chr3.3:95937998-95938017 | None:intergenic | 40.0% | |
| AGTATGTATGCATAGCAGGA+AGG | - | chr3.3:95938593-95938612 | MS.gene063076:CDS | 40.0% | |
| AGTGAGCATTTGGATGGAAT+TGG | + | chr3.3:95937988-95938007 | None:intergenic | 40.0% | |
| ATCAATGATAGAGTGGTCTC+AGG | + | chr3.3:95938376-95938395 | None:intergenic | 40.0% | |
| ATCCATTGTTCCAACACATC+AGG | + | chr3.3:95939220-95939239 | None:intergenic | 40.0% | |
| ATGACATCATCTGAACCAAC+CGG | + | chr3.3:95939178-95939197 | None:intergenic | 40.0% | |
| ATGCATACATACTTGAGGTG+AGG | + | chr3.3:95938586-95938605 | None:intergenic | 40.0% | |
| ATTTCTTGAACCAGGGGTTA+GGG | - | chr3.3:95938131-95938150 | MS.gene063076:intron | 40.0% | |
| CAGATGATGTCATTAGACCA+TGG | - | chr3.3:95939185-95939204 | MS.gene063076:CDS | 40.0% | |
| CGTCTGTATATGCAGCAATT+TGG | - | chr3.3:95938424-95938443 | MS.gene063076:intron | 40.0% | |
| CTCAAGTATGTATGCATAGC+AGG | - | chr3.3:95938589-95938608 | MS.gene063076:intron | 40.0% | |
| CTGCTATGCATACATACTTG+AGG | + | chr3.3:95938591-95938610 | None:intergenic | 40.0% | |
| GAGACCACTCTATCATTGAT+TGG | - | chr3.3:95938376-95938395 | MS.gene063076:CDS | 40.0% | |
| GATGTCATTAGACCATGGTA+TGG | - | chr3.3:95939190-95939209 | MS.gene063076:CDS | 40.0% | |
| GTAACACACACACTCTTTCA+AGG | - | chr3.3:95938615-95938634 | MS.gene063076:CDS | 40.0% | |
| GTGACCAATCAATGATAGAG+TGG | + | chr3.3:95938383-95938402 | None:intergenic | 40.0% | |
| GTTTGATCGATCCCAATTAC+TGG | - | chr3.3:95938263-95938282 | MS.gene063076:CDS | 40.0% | |
| TATTTCTTGAACCAGGGGTT+AGG | - | chr3.3:95938130-95938149 | MS.gene063076:intron | 40.0% | |
| TCAAGATGAGCTGATACTTC+TGG | + | chr3.3:95939115-95939134 | None:intergenic | 40.0% | |
| TCCATTGTTCCAACACATCA+GGG | + | chr3.3:95939219-95939238 | None:intergenic | 40.0% | |
| TGCATACATACTTGAGGTGA+GGG | + | chr3.3:95938585-95938604 | None:intergenic | 40.0% | |
| TGGTTTGATCGATAACACCA+GGG | + | chr3.3:95938513-95938532 | None:intergenic | 40.0% | |
| ! | CAGTTTGCACACCATAGTTT+TGG | + | chr3.3:95938986-95939005 | None:intergenic | 40.0% |
| ! | GAAGTATCAGCTCATCTTGA+CGG | - | chr3.3:95939115-95939134 | MS.gene063076:intron | 40.0% |
| ! | TTGATAATAGCAGTCTCCTC+GGG | + | chr3.3:95938859-95938878 | None:intergenic | 40.0% |
| !! | AGTTGATGATGCTGTTCAAG+AGG | - | chr3.3:95938333-95938352 | MS.gene063076:CDS | 40.0% |
| !! | TGGTGTTATCGATCAAACCA+GGG | - | chr3.3:95938513-95938532 | MS.gene063076:intron | 40.0% |
| AACATAATCAAGGAGACCCC+TGG | + | chr3.3:95938533-95938552 | None:intergenic | 45.0% | |
| AATGATAGAGTGGTCTCAGG+AGG | + | chr3.3:95938373-95938392 | None:intergenic | 45.0% | |
| ATTGGGATCGATCAAACCAG+CGG | + | chr3.3:95938260-95938279 | None:intergenic | 45.0% | |
| CATTCACCTGCTTGTATCCT+TGG | + | chr3.3:95938933-95938952 | None:intergenic | 45.0% | |
| CCATTGTTCCAACACATCAG+GGG | + | chr3.3:95939218-95939237 | None:intergenic | 45.0% | |
| CGAGCACGCAATGTTATGTT+AGG | + | chr3.3:95938901-95938920 | None:intergenic | 45.0% | |
| CTCCTCACATCGCATTCATT+TGG | - | chr3.3:95938102-95938121 | MS.gene063076:CDS | 45.0% | |
| GAAGACAGTGAGCATTTGGA+TGG | + | chr3.3:95937994-95938013 | None:intergenic | 45.0% | |
| GCACTTAACATTTCCCTGGA+TGG | - | chr3.3:95939139-95939158 | MS.gene063076:intron | 45.0% | |
| GCATACATACTTGAGGTGAG+GGG | + | chr3.3:95938584-95938603 | None:intergenic | 45.0% | |
| GCATTTGGATGGAATTGGAG+AGG | + | chr3.3:95937983-95938002 | None:intergenic | 45.0% | |
| TGACATCATCTGAACCAACC+GGG | + | chr3.3:95939177-95939196 | None:intergenic | 45.0% | |
| TTGTGGGCAAGCAGATGTAT+GGG | - | chr3.3:95939075-95939094 | MS.gene063076:intron | 45.0% | |
| TTTGTGGGCAAGCAGATGTA+TGG | - | chr3.3:95939074-95939093 | MS.gene063076:intron | 45.0% | |
| ! | CTGGTTTGATCGATAACACC+AGG | + | chr3.3:95938514-95938533 | None:intergenic | 45.0% |
| ! | GAAGTATCTGTAGGAAGTGC+AGG | + | chr3.3:95938826-95938845 | None:intergenic | 45.0% |
| ! | GGTGTTATCGATCAAACCAG+GGG | - | chr3.3:95938514-95938533 | MS.gene063076:intron | 45.0% |
| !! | AGTAGCTGGGAAGTATCTGT+AGG | + | chr3.3:95938835-95938854 | None:intergenic | 45.0% |
| !! | CTGGTGTTATCGATCAAACC+AGG | - | chr3.3:95938512-95938531 | MS.gene063076:intron | 45.0% |
| !! | CTTTTGGAGGTGGATGATGA+GGG | + | chr3.3:95938486-95938505 | None:intergenic | 45.0% |
| !! | TTTTGGAGGTGGATGATGAG+GGG | + | chr3.3:95938485-95938504 | None:intergenic | 45.0% |
| AACCAACGCCTGTCCTTGAT+TGG | - | chr3.3:95938299-95938318 | MS.gene063076:CDS | 50.0% | |
| AAGAGTCCCACGCCAGTAAT+TGG | + | chr3.3:95938278-95938297 | None:intergenic | 50.0% | |
| ACATCAGGGGAACCATACCA+TGG | + | chr3.3:95939205-95939224 | None:intergenic | 50.0% | |
| ACATTGCGTGCTCGCTTTGT+TGG | - | chr3.3:95938907-95938926 | MS.gene063076:CDS | 50.0% | |
| AGAGTCCCACGCCAGTAATT+GGG | + | chr3.3:95938277-95938296 | None:intergenic | 50.0% | |
| ATCGATCCCAATTACTGGCG+TGG | - | chr3.3:95938268-95938287 | MS.gene063076:CDS | 50.0% | |
| CAGGGGTTAGGGAACAATTC+CGG | - | chr3.3:95938142-95938161 | MS.gene063076:intron | 50.0% | |
| CCAATCAGGTCTTGACACCT+TGG | + | chr3.3:95938227-95938246 | None:intergenic | 50.0% | |
| CCCCTGATGTGTTGGAACAA+TGG | - | chr3.3:95939215-95939234 | MS.gene063076:CDS | 50.0% | |
| CCTCATCATCCACCTCCAAA+AGG | - | chr3.3:95938484-95938503 | MS.gene063076:intron | 50.0% | |
| CGATCAAACCAGCGGCATTT+CGG | + | chr3.3:95938252-95938271 | None:intergenic | 50.0% | |
| CGGTGCACTTAACATTTCCC+TGG | - | chr3.3:95939135-95939154 | MS.gene063076:intron | 50.0% | |
| GACATCATCTGAACCAACCG+GGG | + | chr3.3:95939176-95939195 | None:intergenic | 50.0% | |
| GAGACTAACCGGGAGAATGA+CGG | + | chr3.3:95938052-95938071 | None:intergenic | 50.0% | |
| GGAGTGGTAAACTCCATCCA+GGG | + | chr3.3:95939155-95939174 | None:intergenic | 50.0% | |
| GTATGGTTCCCCTGATGTGT+TGG | - | chr3.3:95939207-95939226 | MS.gene063076:CDS | 50.0% | |
| TACCAATCAAGGACAGGCGT+TGG | + | chr3.3:95938304-95938323 | None:intergenic | 50.0% | |
| TCGATCCCAATTACTGGCGT+GGG | - | chr3.3:95938269-95938288 | MS.gene063076:CDS | 50.0% | |
| TGACACCTTGGCAACAACTG+TGG | + | chr3.3:95938215-95938234 | None:intergenic | 50.0% | |
| TGATGCTGTTCAAGAGGCCA+TGG | - | chr3.3:95938339-95938358 | MS.gene063076:CDS | 50.0% | |
| TGTGGGCAAGCAGATGTATG+GGG | - | chr3.3:95939076-95939095 | MS.gene063076:intron | 50.0% | |
| TGTTGGCCAAGGATACAAGC+AGG | - | chr3.3:95938924-95938943 | MS.gene063076:CDS | 50.0% | |
| ! | ATAACACCAGGGACGCCTTT+TGG | + | chr3.3:95938502-95938521 | None:intergenic | 50.0% |
| ! | ATTCAAGGGGCTCCCCTTTT+TGG | - | chr3.3:95938778-95938797 | MS.gene063076:CDS | 50.0% |
| ! | CCAAGGTGTCAAGACCTGAT+TGG | - | chr3.3:95938224-95938243 | MS.gene063076:intron | 50.0% |
| ! | TCATTGATTGGTCACTCGGC+TGG | - | chr3.3:95938388-95938407 | MS.gene063076:CDS | 50.0% |
| ! | TTGAGGTGAGGGGTGTAAAC+AGG | + | chr3.3:95938574-95938593 | None:intergenic | 50.0% |
| !! | CCTTTTGGAGGTGGATGATG+AGG | + | chr3.3:95938487-95938506 | None:intergenic | 50.0% |
| ACTAACCGGGAGAATGACGG+CGG | + | chr3.3:95938049-95938068 | None:intergenic | 55.0% | |
| AGTCTCCTCGGGAAGTAGCT+GGG | + | chr3.3:95938848-95938867 | None:intergenic | 55.0% | |
| CAAGCAGATGTATGGGGCGA+TGG | - | chr3.3:95939082-95939101 | MS.gene063076:intron | 55.0% | |
| CGGAATTGTTCCCTAACCCC+TGG | + | chr3.3:95938144-95938163 | None:intergenic | 55.0% | |
| CGTGTCCACAGTTGTTGCCA+AGG | - | chr3.3:95938207-95938226 | MS.gene063076:intron | 55.0% | |
| CTCCAGCTTCTGGTAGTCAC+CGG | + | chr3.3:95938164-95938183 | None:intergenic | 55.0% | |
| GGATGGAGTTTACCACTCCC+CGG | - | chr3.3:95939156-95939175 | MS.gene063076:CDS | 55.0% | |
| GGGAGTGGTAAACTCCATCC+AGG | + | chr3.3:95939156-95939175 | None:intergenic | 55.0% | |
| TACTTCCCAGCTACTTCCCG+AGG | - | chr3.3:95938840-95938859 | MS.gene063076:CDS | 55.0% | |
| TCAGAGTTGCCTCCAGCTTC+TGG | + | chr3.3:95938174-95938193 | None:intergenic | 55.0% | |
| TTCCGGTGACTACCAGAAGC+TGG | - | chr3.3:95938159-95938178 | MS.gene063076:intron | 55.0% | |
| ! | GGAAGTGCAGGCTCAGCATT+TGG | + | chr3.3:95938814-95938833 | None:intergenic | 55.0% |
| ! | TTGATTGGTCACTCGGCTGG+AGG | - | chr3.3:95938391-95938410 | MS.gene063076:CDS | 55.0% |
| !! | CTCAGGAGGTGCTAACTCCA+TGG | + | chr3.3:95938359-95938378 | None:intergenic | 55.0% |
| CAGTCTCCTCGGGAAGTAGC+TGG | + | chr3.3:95938849-95938868 | None:intergenic | 60.0% | |
| CATCTGAACCAACCGGGGAG+TGG | + | chr3.3:95939171-95939190 | None:intergenic | 60.0% | |
| CGGTGACTACCAGAAGCTGG+AGG | - | chr3.3:95938162-95938181 | MS.gene063076:intron | 60.0% | |
| CTAACCGGGAGAATGACGGC+GGG | + | chr3.3:95938048-95938067 | None:intergenic | 60.0% | |
| GACGGCGGGTCGATAGTTGT+TGG | + | chr3.3:95938034-95938053 | None:intergenic | 60.0% | |
| GCGGCATTTCGGAGCCAATC+AGG | + | chr3.3:95938241-95938260 | None:intergenic | 60.0% | |
| GGAGTTTACCACTCCCCGGT+TGG | - | chr3.3:95939160-95939179 | MS.gene063076:CDS | 60.0% | |
| GGCGGGTCGATAGTTGTTGG+TGG | + | chr3.3:95938031-95938050 | None:intergenic | 60.0% | |
| TTGGTCACTCGGCTGGAGGA+TGG | - | chr3.3:95938395-95938414 | MS.gene063076:CDS | 60.0% | |
| ! | ACACCAGGGACGCCTTTTGG+AGG | + | chr3.3:95938499-95938518 | None:intergenic | 60.0% |
| ! | CGTGCTCGCTTTGTTGGCCA+AGG | - | chr3.3:95938913-95938932 | MS.gene063076:CDS | 60.0% |
| ! | GATTGGCTCCGAAATGCCGC+TGG | - | chr3.3:95938241-95938260 | MS.gene063076:intron | 60.0% |
| TCGACCCGCCGTCATTCTCC+CGG | - | chr3.3:95938041-95938060 | MS.gene063076:CDS | 65.0% | |
| !! | CCACCTCCAAAAGGCGTCCC+TGG | - | chr3.3:95938493-95938512 | MS.gene063076:intron | 65.0% |
| !! | CCAGGGACGCCTTTTGGAGG+TGG | + | chr3.3:95938496-95938515 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 95937949 | 95939258 | 95937949 | ID=MS.gene063076 |
| chr3.3 | mRNA | 95937949 | 95939258 | 95937949 | ID=MS.gene063076.t1;Parent=MS.gene063076 |
| chr3.3 | exon | 95939145 | 95939258 | 95939145 | ID=MS.gene063076.t1.exon1;Parent=MS.gene063076.t1 |
| chr3.3 | CDS | 95939145 | 95939258 | 95939145 | ID=cds.MS.gene063076.t1;Parent=MS.gene063076.t1 |
| chr3.3 | exon | 95938593 | 95939062 | 95938593 | ID=MS.gene063076.t1.exon2;Parent=MS.gene063076.t1 |
| chr3.3 | CDS | 95938593 | 95939062 | 95938593 | ID=cds.MS.gene063076.t1;Parent=MS.gene063076.t1 |
| chr3.3 | exon | 95938262 | 95938433 | 95938262 | ID=MS.gene063076.t1.exon3;Parent=MS.gene063076.t1 |
| chr3.3 | CDS | 95938262 | 95938433 | 95938262 | ID=cds.MS.gene063076.t1;Parent=MS.gene063076.t1 |
| chr3.3 | exon | 95937949 | 95938134 | 95937949 | ID=MS.gene063076.t1.exon4;Parent=MS.gene063076.t1 |
| chr3.3 | CDS | 95937949 | 95938134 | 95937949 | ID=cds.MS.gene063076.t1;Parent=MS.gene063076.t1 |
| Gene Sequence |
| Protein sequence |
