AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065724


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065724.t1 MTR_1g058540 96.429 196 7 0 1 196 1 196 3.13e-136 379
MS.gene065724.t1 MTR_1g058570 77.551 196 42 1 1 196 1 194 9.87e-94 271
MS.gene065724.t1 MTR_1g058560 80.612 196 37 1 1 196 1 195 3.01e-92 268
MS.gene065724.t1 MTR_2g086740 52.381 210 84 1 2 195 10 219 7.23e-59 184
MS.gene065724.t1 MTR_7g011090 45.789 190 99 1 7 196 1 186 1.07e-52 167
MS.gene065724.t1 MTR_4g081880 42.197 173 100 0 23 195 14 186 1.79e-47 154
MS.gene065724.t1 MTR_5g041900 40.722 194 102 2 4 196 5 186 1.78e-41 139
MS.gene065724.t1 MTR_2g086740 49.645 141 55 1 2 126 10 150 6.63e-34 118
MS.gene065724.t1 MTR_0004s0210 34.615 156 87 4 37 189 7 150 4.56e-18 77.8
MS.gene065724.t1 MTR_0097s0070 34.167 120 73 3 73 189 69 185 1.73e-12 63.5
MS.gene065724.t1 MTR_2g026530 26.220 164 116 3 33 193 6 167 7.40e-11 58.9
MS.gene065724.t1 MTR_2g026530 26.220 164 116 3 33 193 26 187 8.06e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065724.t1 AT2G36100 55.446 202 83 2 2 196 5 206 9.64e-69 209
MS.gene065724.t1 AT3G11550 60.500 200 74 1 2 196 5 204 1.71e-64 197
MS.gene065724.t1 AT2G27370 56.522 184 73 1 12 195 43 219 2.17e-64 198
MS.gene065724.t1 AT5G06200 58.586 198 77 2 2 195 5 201 1.00e-63 196
MS.gene065724.t1 AT5G15290 44.000 150 84 0 27 176 17 166 5.02e-44 145
MS.gene065724.t1 AT1G14160 40.606 165 98 0 32 196 45 209 2.58e-42 141
MS.gene065724.t1 AT1G03700 30.769 169 99 3 21 189 1 151 2.05e-17 76.3
MS.gene065724.t1 AT1G17200 26.455 189 126 5 9 193 2 181 5.95e-14 67.8
MS.gene065724.t1 AT4G03540 32.258 124 75 2 55 178 26 140 1.19e-13 66.2
MS.gene065724.t1 AT4G15630 26.816 179 111 5 27 193 19 189 4.02e-11 59.7
MS.gene065724.t1 AT3G06390 27.820 133 91 2 38 165 37 169 5.83e-11 59.7

Find 42 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCCATTTGCAACCAATT+TGG 0.187527 1.2:-45956023 MS.gene065724:CDS
GTGATGTCTGCTTTGGCTCT+TGG 0.283856 1.2:-45955927 MS.gene065724:CDS
TCTTAGAACAAAATCCATTA+TGG 0.318159 1.2:+45957087 None:intergenic
ATGATGGTTACTATGGAAAA+TGG 0.329026 1.2:+45956678 None:intergenic
TGATGTCTGCTTTGGCTCTT+GGG 0.345325 1.2:-45955926 MS.gene065724:CDS
AGCAGCTGCCATGGTTTACT+TGG 0.359773 1.2:-45956075 MS.gene065724:CDS
GTTGGTTGTGATGTCTGCTT+TGG 0.399140 1.2:-45955934 MS.gene065724:CDS
GGCATTTGTGGGTGGCTACC+TGG 0.401958 1.2:-45956712 MS.gene065724:CDS
AATGTCAACCACTATTGATA+TGG 0.415102 1.2:-45957194 MS.gene065724:CDS
AGGGCGAATGATGGTTACTA+TGG 0.422728 1.2:+45956671 None:intergenic
CATAACTAGCTTCAAATTGA+AGG 0.426220 1.2:+45956984 None:intergenic
ATTGGAGCCACTGCAAGGCC+TGG 0.429435 1.2:-45957130 MS.gene065724:CDS
ACGGCAATCAAGACTCAAAC+TGG 0.435689 1.2:-45956046 MS.gene065724:CDS
ATCTCCAAATTGGTTGCAAA+TGG 0.439108 1.2:+45956019 None:intergenic
TGTTATTGCAATGGCATTTG+TGG 0.446696 1.2:-45956724 MS.gene065724:CDS
GTTATTGCAATGGCATTTGT+GGG 0.458726 1.2:-45956723 MS.gene065724:CDS
ATCATTCGCCCTCATGCACC+TGG 0.484461 1.2:-45956660 MS.gene065724:CDS
TGGTTACTATGGAAAATGGT+AGG 0.486275 1.2:+45956682 None:intergenic
TGGTGCTTCAGCAGCTGCCA+TGG 0.489524 1.2:-45956084 MS.gene065724:CDS
TGAAGGAACTGAGTGAAGAA+AGG 0.493692 1.2:+45957001 None:intergenic
TGACTCTGCCATATCAATAG+TGG 0.510355 1.2:+45957186 None:intergenic
CCACTGCAAGGCCTGGAGGA+TGG 0.524990 1.2:-45957123 MS.gene065724:CDS
TGCAGCAAGAGAAGCTGCTA+TGG 0.527344 1.2:+45957057 None:intergenic
CCATCCTCCAGGCCTTGCAG+TGG 0.532661 1.2:+45957123 None:intergenic
ATGAGGAAAAGCCTTGGTCC+AGG 0.542585 1.2:+45956642 None:intergenic
CTTTAATTGGAGCCACTGCA+AGG 0.554612 1.2:-45957135 MS.gene065724:CDS
GGTTACTATGGAAAATGGTA+GGG 0.561081 1.2:+45956683 None:intergenic
AGCCTTGGTCCAGGTGCATG+AGG 0.573726 1.2:+45956651 None:intergenic
AGGTGCATGAGGGCGAATGA+TGG 0.573876 1.2:+45956662 None:intergenic
GCCCTCATGCACCTGGACCA+AGG 0.587519 1.2:-45956653 MS.gene065724:CDS
TGCTGAAGCACCACTAGCAG+TGG 0.592321 1.2:+45956094 None:intergenic
AATCTGATCACTAGTTGCCA+TGG 0.596028 1.2:+45957027 None:intergenic
ATTGCAATGGCATTTGTGGG+TGG 0.596308 1.2:-45956720 MS.gene065724:CDS
ATGACATTAGCCACTGCTAG+TGG 0.602800 1.2:-45956104 MS.gene065724:CDS
GCGATGATGAGGAAAAGCCT+TGG 0.613791 1.2:+45956636 None:intergenic
GCCTTGGTCCAGGTGCATGA+GGG 0.623258 1.2:+45956652 None:intergenic
ATGGTTTACTTGGCACACAA+CGG 0.636706 1.2:-45956065 MS.gene065724:CDS
GGAGCCACTGCAAGGCCTGG+AGG 0.665040 1.2:-45957127 MS.gene065724:CDS
TTACTGTGTCTGCGATGATG+AGG 0.667059 1.2:+45956625 None:intergenic
TCTTGCTGCAACTATCACCA+TGG 0.694318 1.2:-45957044 MS.gene065724:CDS
AAAATGGTAGGGATAAGACC+AGG 0.708096 1.2:+45956694 None:intergenic
GTTGTGTGCCAAGTAAACCA+TGG 0.790091 1.2:+45956067 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAATCAATAAAGCAA+TGG + chr1.2:45956675-45956694 None:intergenic 15.0%
!! ACTCTAATTAAGAATTAATT+AGG + chr1.2:45956526-45956545 None:intergenic 15.0%
!! TAAGAATTAATTAGGAAATT+AGG + chr1.2:45956518-45956537 None:intergenic 15.0%
!! TCATGTAAAAAAAATTTGAA+GGG + chr1.2:45956569-45956588 None:intergenic 15.0%
!! TTCATGTAAAAAAAATTTGA+AGG + chr1.2:45956570-45956589 None:intergenic 15.0%
!!! AAATTATTTGTTTAAGTTAG+AGG + chr1.2:45956187-45956206 None:intergenic 15.0%
!! AATGTTTATTGTAAATATGC+AGG - chr1.2:45956358-45956377 MS.gene065724:intron 20.0%
!! ATGAACATATATATTCTAGT+AGG - chr1.2:45956585-45956604 MS.gene065724:intron 20.0%
!!! GTTAAGTACTTTTAAAAAAC+TGG - chr1.2:45956867-45956886 MS.gene065724:intron 20.0%
! GGTCATTTGAAAATAAATAG+AGG + chr1.2:45956654-45956673 None:intergenic 25.0%
! TCTTAGAACAAAATCCATTA+TGG + chr1.2:45956026-45956045 None:intergenic 25.0%
!! AATGAAAAAGCTGCTTTAAT+TGG - chr1.2:45955962-45955981 MS.gene065724:CDS 25.0%
!!! ATGGATTTTGTTCTAAGATT+AGG - chr1.2:45956028-45956047 MS.gene065724:CDS 25.0%
AATGAGGAAATGAACTATGA+AGG - chr1.2:45956788-45956807 MS.gene065724:intron 30.0%
ATGAGGAAATGAACTATGAA+GGG - chr1.2:45956789-45956808 MS.gene065724:intron 30.0%
ATGATGGTTACTATGGAAAA+TGG + chr1.2:45956435-45956454 None:intergenic 30.0%
ATTACTCCTTGTGTTTCTTT+CGG - chr1.2:45956628-45956647 MS.gene065724:intron 30.0%
GAAGATAAATTGACACAATG+AGG - chr1.2:45956772-45956791 MS.gene065724:intron 30.0%
TAATGTCATAAACACCTACA+CGG + chr1.2:45956995-45957014 None:intergenic 30.0%
TGGACTAAATAAATACACCT+AGG + chr1.2:45956232-45956251 None:intergenic 30.0%
! AATACACCTAGGTTTTACAA+TGG + chr1.2:45956221-45956240 None:intergenic 30.0%
! CATAACTAGCTTCAAATTGA+AGG + chr1.2:45956129-45956148 None:intergenic 30.0%
! GTGTAGTTCAAATTTTGTGT+TGG + chr1.2:45956252-45956271 None:intergenic 30.0%
!!! CAGGTTTTTTGTTATTGCAA+TGG - chr1.2:45956377-45956396 MS.gene065724:intron 30.0%
!!! TATTGCTGTGGTTGTTTTAA+TGG - chr1.2:45957152-45957171 MS.gene065724:CDS 30.0%
ATCTCCAAATTGGTTGCAAA+TGG + chr1.2:45957094-45957113 None:intergenic 35.0%
GAGGTTTAAATGTGACATAC+TGG + chr1.2:45956168-45956187 None:intergenic 35.0%
GGTTACTATGGAAAATGGTA+GGG + chr1.2:45956430-45956449 None:intergenic 35.0%
GTGCACAAAAATCTCCAAAT+TGG + chr1.2:45957104-45957123 None:intergenic 35.0%
GTTATTGCAATGGCATTTGT+GGG - chr1.2:45956387-45956406 MS.gene065724:intron 35.0%
TGGTTACTATGGAAAATGGT+AGG + chr1.2:45956431-45956450 None:intergenic 35.0%
TGTTATTGCAATGGCATTTG+TGG - chr1.2:45956386-45956405 MS.gene065724:intron 35.0%
! AAACTCAAACTTTTCCGTGT+AGG - chr1.2:45956978-45956997 MS.gene065724:CDS 35.0%
! GAAAAAAGGCATAGCCATAA+TGG - chr1.2:45956009-45956028 MS.gene065724:CDS 35.0%
!! TGTGTCATGTTTTATTGCTG+TGG - chr1.2:45957140-45957159 MS.gene065724:CDS 35.0%
!!! TGTGGTTGTTTTAATGGTGT+TGG - chr1.2:45957158-45957177 MS.gene065724:CDS 35.0%
AAAATGGTAGGGATAAGACC+AGG + chr1.2:45956419-45956438 None:intergenic 40.0%
AATCTGATCACTAGTTGCCA+TGG + chr1.2:45956086-45956105 None:intergenic 40.0%
ATGGTTTACTTGGCACACAA+CGG - chr1.2:45957045-45957064 MS.gene065724:CDS 40.0%
TGAAGGAACTGAGTGAAGAA+AGG + chr1.2:45956112-45956131 None:intergenic 40.0%
TGACTCTGCCATATCAATAG+TGG + chr1.2:45955927-45955946 None:intergenic 40.0%
! ACACCTAGGTTTTACAATGG+AGG + chr1.2:45956218-45956237 None:intergenic 40.0%
! CACCTAGGTTTTACAATGGA+GGG + chr1.2:45956217-45956236 None:intergenic 40.0%
! CTTGCCATTTGCAACCAATT+TGG - chr1.2:45957087-45957106 MS.gene065724:CDS 40.0%
! GATTTTTGTGCACAGACAAG+TGG - chr1.2:45957111-45957130 MS.gene065724:CDS 40.0%
ACGGCAATCAAGACTCAAAC+TGG - chr1.2:45957064-45957083 MS.gene065724:CDS 45.0%
AGGGCGAATGATGGTTACTA+TGG + chr1.2:45956442-45956461 None:intergenic 45.0%
ATGACATTAGCCACTGCTAG+TGG - chr1.2:45957006-45957025 MS.gene065724:CDS 45.0%
ATTGCAATGGCATTTGTGGG+TGG - chr1.2:45956390-45956409 MS.gene065724:intron 45.0%
CTCCCTCCATTGTAAAACCT+AGG - chr1.2:45956212-45956231 MS.gene065724:intron 45.0%
CTTTAATTGGAGCCACTGCA+AGG - chr1.2:45955975-45955994 MS.gene065724:CDS 45.0%
GTTGGTTGTGATGTCTGCTT+TGG - chr1.2:45957176-45957195 MS.gene065724:CDS 45.0%
GTTGTGTGCCAAGTAAACCA+TGG + chr1.2:45957046-45957065 None:intergenic 45.0%
TAGAGGCCGAAAGAAACACA+AGG + chr1.2:45956637-45956656 None:intergenic 45.0%
TCTTGCTGCAACTATCACCA+TGG - chr1.2:45956066-45956085 MS.gene065724:CDS 45.0%
TTACTGTGTCTGCGATGATG+AGG + chr1.2:45956488-45956507 None:intergenic 45.0%
!! TGATGTCTGCTTTGGCTCTT+GGG - chr1.2:45957184-45957203 MS.gene065724:CDS 45.0%
!!! ATGCCTTTTTTCCATCCTCC+AGG + chr1.2:45956001-45956020 None:intergenic 45.0%
AGCAGCTGCCATGGTTTACT+TGG - chr1.2:45957035-45957054 MS.gene065724:CDS 50.0%
AGGCCTGGAGGATGGAAAAA+AGG - chr1.2:45955995-45956014 MS.gene065724:CDS 50.0%
ATGAGGAAAAGCCTTGGTCC+AGG + chr1.2:45956471-45956490 None:intergenic 50.0%
GCGATGATGAGGAAAAGCCT+TGG + chr1.2:45956477-45956496 None:intergenic 50.0%
TGCAGCAAGAGAAGCTGCTA+TGG + chr1.2:45956056-45956075 None:intergenic 50.0%
!! GTGATGTCTGCTTTGGCTCT+TGG - chr1.2:45957183-45957202 MS.gene065724:CDS 50.0%
AGGTGCATGAGGGCGAATGA+TGG + chr1.2:45956451-45956470 None:intergenic 55.0%
ATCATTCGCCCTCATGCACC+TGG - chr1.2:45956450-45956469 MS.gene065724:intron 55.0%
! TGCTGAAGCACCACTAGCAG+TGG + chr1.2:45957019-45957038 None:intergenic 55.0%
AGCCTTGGTCCAGGTGCATG+AGG + chr1.2:45956462-45956481 None:intergenic 60.0%
ATTGGAGCCACTGCAAGGCC+TGG - chr1.2:45955980-45955999 MS.gene065724:CDS 60.0%
GCCTTGGTCCAGGTGCATGA+GGG + chr1.2:45956461-45956480 None:intergenic 60.0%
GGCATTTGTGGGTGGCTACC+TGG - chr1.2:45956398-45956417 MS.gene065724:intron 60.0%
TGGTGCTTCAGCAGCTGCCA+TGG - chr1.2:45957026-45957045 MS.gene065724:CDS 60.0%
CCACTGCAAGGCCTGGAGGA+TGG - chr1.2:45955987-45956006 MS.gene065724:CDS 65.0%
CCATCCTCCAGGCCTTGCAG+TGG + chr1.2:45955990-45956009 None:intergenic 65.0%
GCCCTCATGCACCTGGACCA+AGG - chr1.2:45956457-45956476 MS.gene065724:intron 65.0%
GGAGCCACTGCAAGGCCTGG+AGG - chr1.2:45955983-45956002 MS.gene065724:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 45955917 45957215 45955917 ID=MS.gene065724
chr1.2 mRNA 45955917 45957215 45955917 ID=MS.gene065724.t1;Parent=MS.gene065724
chr1.2 exon 45956965 45957215 45956965 ID=MS.gene065724.t1.exon1;Parent=MS.gene065724.t1
chr1.2 CDS 45956965 45957215 45956965 ID=cds.MS.gene065724.t1;Parent=MS.gene065724.t1
chr1.2 exon 45956629 45956752 45956629 ID=MS.gene065724.t1.exon2;Parent=MS.gene065724.t1
chr1.2 CDS 45956629 45956752 45956629 ID=cds.MS.gene065724.t1;Parent=MS.gene065724.t1
chr1.2 exon 45955917 45956132 45955917 ID=MS.gene065724.t1.exon3;Parent=MS.gene065724.t1
chr1.2 CDS 45955917 45956132 45955917 ID=cds.MS.gene065724.t1;Parent=MS.gene065724.t1
Gene Sequence

>MS.gene065724

ATGTCAACCACTATTGATATGGCAGAGTCAAGCAACGCTCATGTTAATGAAAAAGCTGCTTTAATTGGAGCCACTGCAAGGCCTGGAGGATGGAAAAAAGGCATAGCCATAATGGATTTTGTTCTAAGATTAGGTGCCATAGCAGCTTCTCTTGCTGCAACTATCACCATGGCAACTAGTGATCAGATTCTTCCTTTCTTCACTCAGTTCCTTCAATTTGAAGCTAGTTATGATAGCTTTTCAACTTTCCAGTATGTCACATTTAAACCTCTAACTTAAACAAATAATTTATATACTCCCTCCATTGTAAAACCTAGGTGTATTTATTTAGTCCAACACAAAATTTGAACTACACACATATTTATATACTTTTTATTTATATTTTGTTATTAATTTTGTGCTATATTCATAGAAATTAAATTAATTATGTCAAAAAATATTAATGTTTATTGTAAATATGCAGGTTTTTTGTTATTGCAATGGCATTTGTGGGTGGCTACCTGGTCTTATCCCTACCATTTTCCATAGTAACCATCATTCGCCCTCATGCACCTGGACCAAGGCTTTTCCTCATCATCGCAGACACAGTAATTATGCTCCTAATTTCCTAATTAATTCTTAATTAGAGTAAAATAATTTTACTTGAGAACCCTTCAAATTTTTTTTACATGAACATATATATTCTAGTAGGAACACATTTTAAATATAATTATTACTCCTTGTGTTTCTTTCGGCCTCTATTTATTTTCAAATGACCATTGCTTTATTGATTTTTTTTTAATATTCACATGTCATTTTCAAGACTAAAATTACATTTTTTTTGTTGCAAAGTATACTCTTGATTATTTATTGCAAGAAGATAAATTGACACAATGAGGAAATGAACTATGAAGGGTATAACAAGAAAAATACTAACAATTTTATAAAAAAAAAATATATTGATTTATTTAGTTAAGTACTTTTAAAAAACTGGAAAAAGAGAAACTATTATTTTTGAAGTAGTACTAGTGAATGTTTTATAATTATTTTTGTAAAGATTTGAAATTTTAGATTAAAGTTACAAACTCAAACTTTTCCGTGTAGGTGTTTATGACATTAGCCACTGCTAGTGGTGCTTCAGCAGCTGCCATGGTTTACTTGGCACACAACGGCAATCAAGACTCAAACTGGCTTGCCATTTGCAACCAATTTGGAGATTTTTGTGCACAGACAAGTGGTGCAGTTGTGTCATGTTTTATTGCTGTGGTTGTTTTAATGGTGTTGGTTGTGATGTCTGCTTTGGCTCTTGGGAAGCATTGA

Protein sequence

>MS.gene065724.t1

MSTTIDMAESSNAHVNEKAALIGATARPGGWKKGIAIMDFVLRLGAIAASLAATITMATSDQILPFFTQFLQFEASYDSFSTFQFFVIAMAFVGGYLVLSLPFSIVTIIRPHAPGPRLFLIIADTVFMTLATASGASAAAMVYLAHNGNQDSNWLAICNQFGDFCAQTSGAVVSCFIAVVVLMVLVVMSALALGKH