Alfalfa Gene Editing Database
Nr:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene068082.t1 | XP_003624871.1 | 89.4 | 141 | 15 | 0 | 1 | 141 | 1 | 141 | 3.70E-29 | 137.9 |
Swissprot:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene068082.t1 | P11891 | 51.0 | 145 | 67 | 3 | 1 | 141 | 1 | 145 | 5.0e-19 | 95.5 |
Trembl:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene068082.t1 | B7FHB7 | 89.4 | 141 | 15 | 0 | 1 | 141 | 1 | 141 | 2.6e-29 | 137.9 |
TFs/TRs:
Gene ID | Type | Classification |
---|
Protein Kinases:
Gene ID | Type | Classification |
---|
Network:
Co-expression Network:
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MS.gene049351 | MS.gene068082 | 0.814963 | 1.18E-51 | -1.69E-46 |
MS.gene049427 | MS.gene068082 | 0.820487 | 6.68E-53 | -1.69E-46 |
MS.gene049491 | MS.gene068082 | 0.821669 | 3.57E-53 | -1.69E-46 |
MS.gene049615 | MS.gene068082 | 0.823009 | 1.74E-53 | -1.69E-46 |
MS.gene049661 | MS.gene068082 | 0.820244 | 7.60E-53 | -1.69E-46 |
MS.gene049662 | MS.gene068082 | 0.808189 | 3.50E-50 | -1.69E-46 |
MS.gene050076 | MS.gene068082 | 0.844326 | 8.10E-59 | -1.69E-46 |
MS.gene050099 | MS.gene068082 | 0.810357 | 1.20E-50 | -1.69E-46 |
MS.gene050301 | MS.gene068082 | 0.832507 | 9.09E-56 | -1.69E-46 |
MS.gene050397 | MS.gene068082 | 0.803004 | 4.29E-49 | -1.69E-46 |
MS.gene050432 | MS.gene068082 | 0.808466 | 3.06E-50 | -1.69E-46 |
MS.gene050464 | MS.gene068082 | 0.813765 | 2.17E-51 | -1.69E-46 |
MS.gene050529 | MS.gene068082 | 0.809552 | 1.79E-50 | -1.69E-46 |
MS.gene050593 | MS.gene068082 | 0.80138 | 9.25E-49 | -1.69E-46 |
MS.gene050767 | MS.gene068082 | 0.880345 | 5.93E-70 | -1.69E-46 |
MS.gene050770 | MS.gene068082 | 0.880476 | 5.32E-70 | -1.69E-46 |
MS.gene050783 | MS.gene068082 | 0.816418 | 5.59E-52 | -1.69E-46 |
MS.gene05101 | MS.gene068082 | 0.872021 | 4.38E-67 | -1.69E-46 |
MS.gene05105 | MS.gene068082 | 0.800634 | 1.31E-48 | -1.69E-46 |
MS.gene051387 | MS.gene068082 | 0.807356 | 5.26E-50 | -1.69E-46 |
MS.gene051471 | MS.gene068082 | 0.822869 | 1.88E-53 | -1.69E-46 |
MS.gene051666 | MS.gene068082 | 0.820914 | 5.33E-53 | -1.69E-46 |
MS.gene051692 | MS.gene068082 | 0.827353 | 1.64E-54 | -1.69E-46 |
MS.gene051877 | MS.gene068082 | 0.837022 | 6.64E-57 | -1.69E-46 |
MS.gene052078 | MS.gene068082 | 0.861386 | 1.06E-63 | -1.69E-46 |
MS.gene052178 | MS.gene068082 | 0.812644 | 3.82E-51 | -1.69E-46 |
MS.gene052271 | MS.gene068082 | 0.818471 | 1.93E-52 | -1.69E-46 |
MS.gene052290 | MS.gene068082 | 0.806335 | 8.65E-50 | -1.69E-46 |
MS.gene052310 | MS.gene068082 | 0.853413 | 2.43E-61 | -1.69E-46 |
MS.gene052322 | MS.gene068082 | 0.831086 | 2.04E-55 | -1.69E-46 |
MS.gene052326 | MS.gene068082 | 0.845201 | 4.71E-59 | -1.69E-46 |
MS.gene052484 | MS.gene068082 | 0.808413 | 3.14E-50 | -1.69E-46 |
MS.gene053103 | MS.gene068082 | 0.831279 | 1.83E-55 | -1.69E-46 |
MS.gene053104 | MS.gene068082 | 0.819349 | 1.22E-52 | -1.69E-46 |
MS.gene053322 | MS.gene068082 | 0.870494 | 1.40E-66 | -1.69E-46 |
MS.gene054123 | MS.gene068082 | 0.811481 | 6.85E-51 | -1.69E-46 |
MS.gene054376 | MS.gene068082 | 0.890829 | 6.91E-74 | -1.69E-46 |
MS.gene05455 | MS.gene068082 | 0.830375 | 3.04E-55 | -1.69E-46 |
MS.gene054802 | MS.gene068082 | 0.881125 | 3.11E-70 | -1.69E-46 |
MS.gene054803 | MS.gene068082 | 0.878254 | 3.26E-69 | -1.69E-46 |
MS.gene054804 | MS.gene068082 | 0.882184 | 1.29E-70 | -1.69E-46 |
MS.gene054955 | MS.gene068082 | 0.810196 | 1.30E-50 | -1.69E-46 |
MS.gene055116 | MS.gene068082 | 0.813113 | 3.02E-51 | -1.69E-46 |
MS.gene055254 | MS.gene068082 | 0.847333 | 1.24E-59 | -1.69E-46 |
MS.gene055277 | MS.gene068082 | 0.834433 | 3.01E-56 | -1.69E-46 |
MS.gene055390 | MS.gene068082 | 0.809654 | 1.70E-50 | -1.69E-46 |
MS.gene055397 | MS.gene068082 | 0.821804 | 3.32E-53 | -1.69E-46 |
MS.gene055422 | MS.gene068082 | 0.829792 | 4.22E-55 | -1.69E-46 |
MS.gene055428 | MS.gene068082 | 0.835975 | 1.23E-56 | -1.69E-46 |
MS.gene055499 | MS.gene068082 | 0.856166 | 3.87E-62 | -1.69E-46 |
MS.gene055636 | MS.gene068082 | 0.814945 | 1.19E-51 | -1.69E-46 |
MS.gene055647 | MS.gene068082 | 0.844394 | 7.77E-59 | -1.69E-46 |
MS.gene055712 | MS.gene068082 | 0.82347 | 1.36E-53 | -1.69E-46 |
MS.gene055896 | MS.gene068082 | 0.805289 | 1.43E-49 | -1.69E-46 |
MS.gene05592 | MS.gene068082 | 0.82739 | 1.61E-54 | -1.69E-46 |
MS.gene055930 | MS.gene068082 | 0.825211 | 5.30E-54 | -1.69E-46 |
MS.gene056191 | MS.gene068082 | 0.825576 | 4.35E-54 | -1.69E-46 |
MS.gene056407 | MS.gene068082 | 0.841303 | 5.16E-58 | -1.69E-46 |
MS.gene056410 | MS.gene068082 | 0.849701 | 2.73E-60 | -1.69E-46 |
MS.gene056796 | MS.gene068082 | 0.809865 | 1.53E-50 | -1.69E-46 |
MS.gene057011 | MS.gene068082 | 0.809391 | 1.94E-50 | -1.69E-46 |
MS.gene05702 | MS.gene068082 | 0.826654 | 2.41E-54 | -1.69E-46 |
MS.gene057123 | MS.gene068082 | 0.839968 | 1.15E-57 | -1.69E-46 |
MS.gene057129 | MS.gene068082 | 0.85504 | 8.24E-62 | -1.69E-46 |
MS.gene057146 | MS.gene068082 | 0.822601 | 2.17E-53 | -1.69E-46 |
MS.gene057295 | MS.gene068082 | 0.888639 | 4.94E-73 | -1.69E-46 |
MS.gene05733 | MS.gene068082 | 0.805093 | 1.58E-49 | -1.69E-46 |
MS.gene057416 | MS.gene068082 | 0.83505 | 2.10E-56 | -1.69E-46 |
MS.gene058006 | MS.gene068082 | 0.806696 | 7.26E-50 | -1.69E-46 |
MS.gene058019 | MS.gene068082 | 0.866982 | 1.91E-65 | -1.69E-46 |
MS.gene05811 | MS.gene068082 | 0.811489 | 6.83E-51 | -1.69E-46 |
MS.gene058233 | MS.gene068082 | 0.801284 | 9.68E-49 | -1.69E-46 |
MS.gene058293 | MS.gene068082 | 0.878654 | 2.36E-69 | -1.69E-46 |
MS.gene058660 | MS.gene068082 | 0.869297 | 3.44E-66 | -1.69E-46 |
MS.gene058787 | MS.gene068082 | 0.830909 | 2.25E-55 | -1.69E-46 |
MS.gene058903 | MS.gene068082 | 0.808253 | 3.39E-50 | -1.69E-46 |
MS.gene058993 | MS.gene068082 | 0.809249 | 2.08E-50 | -1.69E-46 |
MS.gene05907 | MS.gene068082 | 0.837851 | 4.07E-57 | -1.69E-46 |
MS.gene059326 | MS.gene068082 | 0.857321 | 1.77E-62 | -1.69E-46 |
MS.gene059536 | MS.gene068082 | 0.851251 | 1.00E-60 | -1.69E-46 |
MS.gene059597 | MS.gene068082 | 0.843399 | 1.44E-58 | -1.69E-46 |
MS.gene059676 | MS.gene068082 | 0.850772 | 1.37E-60 | -1.69E-46 |
MS.gene059688 | MS.gene068082 | 0.809604 | 1.74E-50 | -1.69E-46 |
MS.gene059692 | MS.gene068082 | 0.821865 | 3.22E-53 | -1.69E-46 |
MS.gene059695 | MS.gene068082 | 0.826368 | 2.82E-54 | -1.69E-46 |
MS.gene059894 | MS.gene068082 | 0.805084 | 1.58E-49 | -1.69E-46 |
MS.gene059960 | MS.gene068082 | 0.871763 | 5.33E-67 | -1.69E-46 |
MS.gene060005 | MS.gene068082 | 0.815628 | 8.39E-52 | -1.69E-46 |
MS.gene060193 | MS.gene068082 | 0.833796 | 4.34E-56 | -1.69E-46 |
MS.gene06037 | MS.gene068082 | 0.81208 | 5.08E-51 | -1.69E-46 |
MS.gene060403 | MS.gene068082 | 0.828408 | 9.14E-55 | -1.69E-46 |
MS.gene060804 | MS.gene068082 | 0.817876 | 2.63E-52 | -1.69E-46 |
MS.gene060818 | MS.gene068082 | 0.809648 | 1.71E-50 | -1.69E-46 |
MS.gene061049 | MS.gene068082 | 0.824939 | 6.15E-54 | -1.69E-46 |
MS.gene061200 | MS.gene068082 | 0.874462 | 6.63E-68 | -1.69E-46 |
PPI:
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene068082.t1 | MTR_7g088520 | 92.199 | 141 | 11 | 0 | 1 | 141 | 1 | 141 | 1.70e-88 | 254 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Find 36 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
CTTGGGGTTGTGGAGGGAAT+TGG | 0.246105 | 7.3:-20921855 | None:intergenic |
TGTGGAGGGAATTGGGAAAA+TGG | 0.293091 | 7.3:-20921847 | None:intergenic |
TTGGGGTTGTGGAGGGAATT+GGG | 0.318138 | 7.3:-20921854 | None:intergenic |
TTCTGTAGATAAGCTTCCTT+TGG | 0.349663 | 7.3:+20922162 | MS.gene068082:CDS |
AGTTGAGGAAGAAGGGTAAT+TGG | 0.362329 | 7.3:+20922278 | MS.gene068082:CDS |
TTCAGACCCATCTGCAACAT+TGG | 0.400037 | 7.3:-20922110 | None:intergenic |
AAGAGGAAGTTGAGGAAGAA+GGG | 0.402527 | 7.3:+20922271 | MS.gene068082:CDS |
TCCTTTGGAATCAAAATTGA+AGG | 0.438799 | 7.3:+20922177 | MS.gene068082:CDS |
AAAGAGGAAGTTGAGGAAGA+AGG | 0.452118 | 7.3:+20922270 | MS.gene068082:CDS |
AAGTCCCTTCAATGAGTTTG+TGG | 0.462466 | 7.3:-20922017 | None:intergenic |
ATGGTACTTACTTGGGGTTG+TGG | 0.470559 | 7.3:-20921865 | None:intergenic |
AAATGGAGGAAGAAGAAAGA+GGG | 0.478465 | 7.3:-20921830 | None:intergenic |
GAAAGAGACTTGTTCAAAAG+AGG | 0.482878 | 7.3:+20922254 | MS.gene068082:CDS |
TTGATAAGAAGATAAGGTTG+AGG | 0.486048 | 7.3:+20922230 | MS.gene068082:CDS |
TGGAATCAAAATTGAAGGAG+AGG | 0.491503 | 7.3:+20922182 | MS.gene068082:CDS |
TGAAGCTTGATAAGAAGATA+AGG | 0.493499 | 7.3:+20922224 | MS.gene068082:CDS |
TCCCTCGGTTTCACAACAAA+GGG | 0.494277 | 7.3:-20922052 | None:intergenic |
AGAAAGAGGGGTGAGTGTGA+TGG | 0.496412 | 7.3:-20921817 | None:intergenic |
GGAATCAAAATTGAAGGAGA+GGG | 0.508757 | 7.3:+20922183 | MS.gene068082:CDS |
AGTCCCTTCAATGAGTTTGT+GGG | 0.524517 | 7.3:-20922016 | None:intergenic |
GTACTTACTTGGGGTTGTGG+AGG | 0.532059 | 7.3:-20921862 | None:intergenic |
TTTGCCCACAAACTCATTGA+AGG | 0.534777 | 7.3:+20922012 | MS.gene068082:CDS |
TTCCCTCGGTTTCACAACAA+AGG | 0.535738 | 7.3:-20922053 | None:intergenic |
ACTGAATCCAATGTTGCAGA+TGG | 0.538749 | 7.3:+20922103 | MS.gene068082:CDS |
TACTTACTTGGGGTTGTGGA+GGG | 0.541802 | 7.3:-20921861 | None:intergenic |
TGAAGGAGAGGGAAGAGCAG+AGG | 0.551586 | 7.3:+20922194 | MS.gene068082:CDS |
ATTGAAGGGACTTCGAATCT+CGG | 0.553791 | 7.3:+20922027 | MS.gene068082:CDS |
TTGCCCACAAACTCATTGAA+GGG | 0.555855 | 7.3:+20922013 | MS.gene068082:CDS |
AAAATGGAGGAAGAAGAAAG+AGG | 0.565844 | 7.3:-20921831 | None:intergenic |
TTGTTCAAAAGAGGAAGTTG+AGG | 0.577274 | 7.3:+20922263 | MS.gene068082:CDS |
AATGGAGGAAGAAGAAAGAG+GGG | 0.583049 | 7.3:-20921829 | None:intergenic |
CACCCTTTGTTGTGAAACCG+AGG | 0.599124 | 7.3:+20922050 | MS.gene068082:CDS |
GGAGGGAATTGGGAAAATGG+AGG | 0.607476 | 7.3:-20921844 | None:intergenic |
CTGAATCCAATGTTGCAGAT+GGG | 0.613616 | 7.3:+20922104 | MS.gene068082:CDS |
ACCCTTTGTTGTGAAACCGA+GGG | 0.617169 | 7.3:+20922051 | MS.gene068082:CDS |
TCAGTTTCAAGAGTGCACGT+TGG | 0.725975 | 7.3:+20921989 | MS.gene068082:intron |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|---|---|---|---|---|
!! | AACAAGAATAATAAAAACAT+GGG | - | chr7.3:20921925-20921944 | None:intergenic | 15.0% |
!! | TAACAAGAATAATAAAAACA+TGG | - | chr7.3:20921926-20921945 | None:intergenic | 15.0% |
! | AATGTATTGTGGAGAAATTA+AGG | - | chr7.3:20921902-20921921 | None:intergenic | 25.0% |
!! | ATAAAAATTGCATTTTCCCT+CGG | - | chr7.3:20922070-20922089 | None:intergenic | 25.0% |
!!! | ACTCTTCAATTTTTTCATCT+TGG | - | chr7.3:20922311-20922330 | None:intergenic | 25.0% |
!!! | TTCAATTTTTTCATCTTGGA+AGG | - | chr7.3:20922307-20922326 | None:intergenic | 25.0% |
AAAAACATGGGAATGTATTG+TGG | - | chr7.3:20921913-20921932 | None:intergenic | 30.0% | |
AATTAAGGAAGAAGAAGTGA+TGG | - | chr7.3:20921887-20921906 | None:intergenic | 30.0% | |
TGAAGCTTGATAAGAAGATA+AGG | + | chr7.3:20922224-20922243 | MS.gene068082:CDS | 30.0% | |
TTGATAAGAAGATAAGGTTG+AGG | + | chr7.3:20922230-20922249 | MS.gene068082:CDS | 30.0% | |
!! | TCCTTTGGAATCAAAATTGA+AGG | + | chr7.3:20922177-20922196 | MS.gene068082:CDS | 30.0% |
!!! | AATTTTTTCATCTTGGAAGG+TGG | - | chr7.3:20922304-20922323 | None:intergenic | 30.0% |
!!! | TCCTTCAATTTTGATTCCAA+AGG | - | chr7.3:20922181-20922200 | None:intergenic | 30.0% |
AAAATGGAGGAAGAAGAAAG+AGG | - | chr7.3:20921834-20921853 | None:intergenic | 35.0% | |
AAATGGAGGAAGAAGAAAGA+GGG | - | chr7.3:20921833-20921852 | None:intergenic | 35.0% | |
AAGAAGTGATGGTACTTACT+TGG | - | chr7.3:20921876-20921895 | None:intergenic | 35.0% | |
AGAAGTGATGGTACTTACTT+GGG | - | chr7.3:20921875-20921894 | None:intergenic | 35.0% | |
GGAATCAAAATTGAAGGAGA+GGG | + | chr7.3:20922183-20922202 | MS.gene068082:CDS | 35.0% | |
TGGAATCAAAATTGAAGGAG+AGG | + | chr7.3:20922182-20922201 | MS.gene068082:CDS | 35.0% | |
TTCTGTAGATAAGCTTCCTT+TGG | + | chr7.3:20922162-20922181 | MS.gene068082:CDS | 35.0% | |
TTGTTCAAAAGAGGAAGTTG+AGG | + | chr7.3:20922263-20922282 | MS.gene068082:CDS | 35.0% | |
! | GAAAGAGACTTGTTCAAAAG+AGG | + | chr7.3:20922254-20922273 | MS.gene068082:CDS | 35.0% |
AAAGAGGAAGTTGAGGAAGA+AGG | + | chr7.3:20922270-20922289 | MS.gene068082:CDS | 40.0% | |
AAGAGGAAGTTGAGGAAGAA+GGG | + | chr7.3:20922271-20922290 | MS.gene068082:CDS | 40.0% | |
AAGTCCCTTCAATGAGTTTG+TGG | - | chr7.3:20922020-20922039 | None:intergenic | 40.0% | |
AATGGAGGAAGAAGAAAGAG+GGG | - | chr7.3:20921832-20921851 | None:intergenic | 40.0% | |
ACTGAATCCAATGTTGCAGA+TGG | + | chr7.3:20922103-20922122 | MS.gene068082:CDS | 40.0% | |
AGTCCCTTCAATGAGTTTGT+GGG | - | chr7.3:20922019-20922038 | None:intergenic | 40.0% | |
AGTTGAGGAAGAAGGGTAAT+TGG | + | chr7.3:20922278-20922297 | MS.gene068082:CDS | 40.0% | |
ATTGAAGGGACTTCGAATCT+CGG | + | chr7.3:20922027-20922046 | MS.gene068082:CDS | 40.0% | |
CTGAATCCAATGTTGCAGAT+GGG | + | chr7.3:20922104-20922123 | MS.gene068082:CDS | 40.0% | |
GAAGTGATGGTACTTACTTG+GGG | - | chr7.3:20921874-20921893 | None:intergenic | 40.0% | |
TTGCCCACAAACTCATTGAA+GGG | + | chr7.3:20922013-20922032 | MS.gene068082:CDS | 40.0% | |
TTTGCCCACAAACTCATTGA+AGG | + | chr7.3:20922012-20922031 | MS.gene068082:CDS | 40.0% | |
ATGGTACTTACTTGGGGTTG+TGG | - | chr7.3:20921868-20921887 | None:intergenic | 45.0% | |
TACTTACTTGGGGTTGTGGA+GGG | - | chr7.3:20921864-20921883 | None:intergenic | 45.0% | |
TCCCTCGGTTTCACAACAAA+GGG | - | chr7.3:20922055-20922074 | None:intergenic | 45.0% | |
TGTGGAGGGAATTGGGAAAA+TGG | - | chr7.3:20921850-20921869 | None:intergenic | 45.0% | |
TTCAGACCCATCTGCAACAT+TGG | - | chr7.3:20922113-20922132 | None:intergenic | 45.0% | |
TTCCCTCGGTTTCACAACAA+AGG | - | chr7.3:20922056-20922075 | None:intergenic | 45.0% | |
! | ACCCTTTGTTGTGAAACCGA+GGG | + | chr7.3:20922051-20922070 | MS.gene068082:CDS | 45.0% |
! | TCAGTTTCAAGAGTGCACGT+TGG | + | chr7.3:20921989-20922008 | MS.gene068082:intron | 45.0% |
AGAAAGAGGGGTGAGTGTGA+TGG | - | chr7.3:20921820-20921839 | None:intergenic | 50.0% | |
GGAGGGAATTGGGAAAATGG+AGG | - | chr7.3:20921847-20921866 | None:intergenic | 50.0% | |
GTACTTACTTGGGGTTGTGG+AGG | - | chr7.3:20921865-20921884 | None:intergenic | 50.0% | |
TTGGGGTTGTGGAGGGAATT+GGG | - | chr7.3:20921857-20921876 | None:intergenic | 50.0% | |
! | CACCCTTTGTTGTGAAACCG+AGG | + | chr7.3:20922050-20922069 | MS.gene068082:CDS | 50.0% |
CTTGGGGTTGTGGAGGGAAT+TGG | - | chr7.3:20921858-20921877 | None:intergenic | 55.0% | |
TGAAGGAGAGGGAAGAGCAG+AGG | + | chr7.3:20922194-20922213 | MS.gene068082:CDS | 55.0% |
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
chr7.3 | gene | 20921795 | 20922336 | 20921795 | ID=MS.gene068082 |
chr7.3 | mRNA | 20921795 | 20922336 | 20921795 | ID=MS.gene068082.t1;Parent=MS.gene068082 |
chr7.3 | exon | 20921795 | 20921876 | 20921795 | ID=MS.gene068082.t1.exon1;Parent=MS.gene068082.t1 |
chr7.3 | CDS | 20921795 | 20921876 | 20921795 | ID=cds.MS.gene068082.t1;Parent=MS.gene068082.t1 |
chr7.3 | exon | 20921993 | 20922336 | 20921993 | ID=MS.gene068082.t1.exon2;Parent=MS.gene068082.t1 |
chr7.3 | CDS | 20921993 | 20922336 | 20921993 | ID=cds.MS.gene068082.t1;Parent=MS.gene068082.t1 |
Gene Sequence |
Protein sequence |