AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068998

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068998.t1 MTR_7g070070 99.200 250 2 0 1 250 1 250 0.0 513
MS.gene068998.t1 MTR_5g016590 98.000 250 5 0 1 250 1 250 0.0 508
MS.gene068998.t1 MTR_1g112250 45.161 217 107 5 5 215 8 218 4.29e-58 184
MS.gene068998.t1 MTR_8g075320 42.500 200 112 3 4 202 3 200 2.44e-53 173
MS.gene068998.t1 MTR_2g016050 41.500 200 114 3 4 202 3 200 4.91e-51 167
MS.gene068998.t1 MTR_0167s0060 38.571 210 126 3 4 213 5 211 1.43e-47 157
MS.gene068998.t1 MTR_8g464460 38.136 236 138 4 5 237 9 239 3.36e-44 149
MS.gene068998.t1 MTR_1g022410 38.785 214 125 3 4 215 5 214 2.48e-43 148
MS.gene068998.t1 MTR_2g060830 37.815 238 136 6 5 237 9 239 2.87e-42 144
MS.gene068998.t1 MTR_6g024135 35.071 211 135 2 5 215 8 216 1.58e-37 132
MS.gene068998.t1 MTR_4g012740 28.968 252 135 6 4 215 236 483 5.74e-22 94.7
MS.gene068998.t1 MTR_6g013300 32.632 190 111 7 33 215 138 317 1.78e-16 77.8
MS.gene068998.t1 MTR_4g057090 29.947 187 119 5 26 207 110 289 2.00e-14 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068998.t1 AT3G22110 90.000 250 25 0 1 250 1 250 1.51e-172 475
MS.gene068998.t1 AT4G15165 72.000 250 28 1 1 250 1 208 7.59e-127 358
MS.gene068998.t1 AT3G14290 45.161 217 107 5 5 215 8 218 2.76e-58 185
MS.gene068998.t1 AT1G53850 44.240 217 109 5 5 215 8 218 2.49e-57 182
MS.gene068998.t1 AT1G53850 44.240 217 109 5 5 215 8 218 2.49e-57 182
MS.gene068998.t1 AT5G66140 42.000 200 113 3 4 202 3 200 8.81e-53 171
MS.gene068998.t1 AT3G51260 42.500 200 112 3 4 202 3 200 1.12e-52 171
MS.gene068998.t1 AT3G51260 42.500 200 112 3 4 202 3 200 1.13e-52 171
MS.gene068998.t1 AT1G16470 37.264 212 130 3 4 215 5 213 9.89e-47 155
MS.gene068998.t1 AT1G16470 37.264 212 130 3 4 215 5 213 9.89e-47 155
MS.gene068998.t1 AT1G79210 37.264 212 130 3 4 215 5 213 1.18e-46 155
MS.gene068998.t1 AT1G79210 37.264 212 130 3 4 215 5 213 1.18e-46 155
MS.gene068998.t1 AT1G79210 37.264 212 130 3 4 215 5 213 1.18e-46 155
MS.gene068998.t1 AT5G42790 39.320 206 119 3 4 207 5 206 3.72e-44 150
MS.gene068998.t1 AT1G47250 38.350 206 121 3 4 207 5 206 2.55e-43 148
MS.gene068998.t1 AT5G35590 40.704 199 114 3 5 200 9 206 3.68e-42 144
MS.gene068998.t1 AT2G05840 38.191 199 119 3 5 200 9 206 8.34e-39 135
MS.gene068998.t1 AT2G05840 38.191 199 119 3 5 200 9 206 1.04e-38 135
MS.gene068998.t1 AT2G05840 38.235 204 115 4 5 205 9 204 5.05e-38 132
MS.gene068998.t1 AT2G27020 33.493 209 137 2 5 213 8 214 2.86e-36 129
MS.gene068998.t1 AT2G27020 33.175 211 139 2 5 215 110 318 3.52e-35 129

Find 62 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTCTCTTTCCTATTTGC+AGG 0.250981 7.2:+33573219 MS.gene068998:CDS
GTCAAAAGACGGGGTTGTTC+TGG 0.306994 7.2:+33572942 MS.gene068998:CDS
ACTAAGCTATTGGTGAAGTC+TGG 0.325538 7.2:+33573528 MS.gene068998:CDS
CTCTTTCCTATTTGCAGGCT+GGG 0.331080 7.2:+33573224 MS.gene068998:CDS
AGCAGTTCAACTTGCATTGA+AGG 0.356948 7.2:+33573389 MS.gene068998:CDS
GGATCCTAGCAGTATTGATT+AGG 0.366704 7.2:-33573083 None:intergenic
AGCAGTGTCTGTTGCTGGTT+GGG 0.371924 7.2:-33573556 None:intergenic
TTCGGTGGTCTCCGACCATT+TGG 0.375556 7.2:+33573198 MS.gene068998:CDS
AGAGAATTTGACTAAGCTAT+TGG 0.375935 7.2:+33573518 MS.gene068998:CDS
GATTGAACCAACTGCTCAAC+AGG 0.380070 7.2:-33573144 None:intergenic
TCTCTTTCCTATTTGCAGGC+TGG 0.384561 7.2:+33573223 MS.gene068998:CDS
GAAGGTTCTGAGTAAAACTA+TGG 0.403623 7.2:+33573407 MS.gene068998:CDS
GGTTGGAAAGCTGGTGCTAT+TGG 0.408203 7.2:+33573300 MS.gene068998:CDS
AGCTAAGCAGTGTCTGTTGC+TGG 0.410039 7.2:-33573561 None:intergenic
GGAAATGCTGGTACTGCAAT+AGG 0.415260 7.2:+33572913 MS.gene068998:CDS
TGACTGTGCTGCCTGGTTGT+TGG 0.416766 7.2:-33573325 None:intergenic
CATGTAGCATGTGCTGTCGC+TGG 0.433619 7.2:+33573039 MS.gene068998:CDS
AGTGATCCAAGCGGAAACTA+TGG 0.434323 7.2:+33573276 MS.gene068998:CDS
AGCACAGTCAATTCTAAAAC+AGG 0.450248 7.2:+33573338 MS.gene068998:CDS
GCTCAACAGGCATTGGCTCT+TGG 0.472402 7.2:-33573131 None:intergenic
TTAGAATTGACTGTGCTGCC+TGG 0.476900 7.2:-33573332 None:intergenic
AACTATGGTGGTTGGAAAGC+TGG 0.479761 7.2:+33573291 MS.gene068998:CDS
GCCAATGCCTGTTGAGCAGT+TGG 0.481970 7.2:+33573137 MS.gene068998:CDS
ACTTGGTACAGACGTCCTTC+AGG 0.486610 7.2:-33572868 None:intergenic
AAGCAAGGATACACACAATT+CGG 0.488228 7.2:+33573180 MS.gene068998:CDS
AAGCAGTGTCTGTTGCTGGT+TGG 0.492638 7.2:-33573557 None:intergenic
CTAAGCTATTGGTGAAGTCT+GGG 0.494770 7.2:+33573529 MS.gene068998:CDS
AGCAGTATTGATTAGGATGT+TGG 0.498265 7.2:-33573076 None:intergenic
TAGGAATCTTGTCAAAAGAC+GGG 0.510970 7.2:+33572932 MS.gene068998:CDS
CAAAGGAGTAACGTTGTGCT+TGG 0.511294 7.2:-33573104 None:intergenic
ATGGAGGCCATCGGAAATGC+TGG 0.514078 7.2:+33572901 MS.gene068998:CDS
CAACCACCATAGTTTCCGCT+TGG 0.517588 7.2:-33573282 None:intergenic
ACCAACTGCTCAACAGGCAT+TGG 0.521861 7.2:-33573138 None:intergenic
CAGGAGAGAATATTGTTGTA+CGG 0.539096 7.2:-33572849 None:intergenic
TGCTATTGGTGCCAACAACC+AGG 0.541336 7.2:+33573314 MS.gene068998:CDS
TGTTCTGGTTGGTGAAAAGA+AGG 0.542861 7.2:+33572957 MS.gene068998:CDS
CAAGCGGAAACTATGGTGGT+TGG 0.545365 7.2:+33573283 MS.gene068998:CDS
AGTTGAGGTTTGAAGCAGCT+TGG 0.546459 7.2:-33572986 None:intergenic
ATGTAGCATGTGCTGTCGCT+GGG 0.546740 7.2:+33573040 MS.gene068998:CDS
AATTCTAAAACAGGACTACA+AGG 0.553889 7.2:+33573347 MS.gene068998:CDS
CCTCCATTGCATACTCCACT+TGG 0.562163 7.2:-33572885 None:intergenic
ATAGGAATCTTGTCAAAAGA+CGG 0.567737 7.2:+33572931 MS.gene068998:CDS
AAAGACGGGGTTGTTCTGGT+TGG 0.578404 7.2:+33572946 MS.gene068998:CDS
ACATCCTAATCAATACTGCT+AGG 0.578961 7.2:+33573079 MS.gene068998:CDS
AATAGGAAAGAGACTCCAAA+TGG 0.587032 7.2:-33573213 None:intergenic
CCAAGTGGAGTATGCAATGG+AGG 0.592642 7.2:+33572885 MS.gene068998:CDS
CATTGGCTCTTGGTAAGCAA+AGG 0.596164 7.2:-33573121 None:intergenic
TGCAGTACCAGCATTTCCGA+TGG 0.599600 7.2:-33572908 None:intergenic
GTACCAAGTGGAGTATGCAA+TGG 0.605368 7.2:+33572882 MS.gene068998:CDS
ACAACAATATTCTCTCCTGA+AGG 0.618706 7.2:+33572853 MS.gene068998:CDS
GGAAAGAGACTCCAAATGGT+CGG 0.621181 7.2:-33573209 None:intergenic
GAGTATGCAATGGAGGCCAT+CGG 0.626386 7.2:+33572892 MS.gene068998:CDS
TCTCTCTGTGATACTAAGCA+AGG 0.640603 7.2:+33573165 MS.gene068998:CDS
GATCCAAGCGGAAACTATGG+TGG 0.659270 7.2:+33573279 MS.gene068998:CDS
CAAGGATACACACAATTCGG+TGG 0.675083 7.2:+33573183 MS.gene068998:CDS
TGAAGGACGTCTGTACCAAG+TGG 0.675276 7.2:+33572870 MS.gene068998:CDS
AGGAATCTTGTCAAAAGACG+GGG 0.680553 7.2:+33572933 MS.gene068998:CDS
ACTACAAGGATGATATCACA+AGG 0.683851 7.2:+33573361 MS.gene068998:CDS
TAGCTTAGTCAAATTCTCTG+AGG 0.695782 7.2:-33573514 None:intergenic
TAAGCTATTGGTGAAGTCTG+GGG 0.701098 7.2:+33573530 MS.gene068998:CDS
CTTTACATGAGTGATCCAAG+CGG 0.758885 7.2:+33573267 MS.gene068998:CDS
CTACAAGGATGATATCACAA+GGG 0.766354 7.2:+33573362 MS.gene068998:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAGAATTTGACTAAGCTAT+TGG + chr7.2:33573518-33573537 MS.gene068998:CDS 30.0%
ATAGGAATCTTGTCAAAAGA+CGG + chr7.2:33572931-33572950 MS.gene068998:CDS 30.0%
!! AATTCTAAAACAGGACTACA+AGG + chr7.2:33573347-33573366 MS.gene068998:CDS 30.0%
AAGCAAGGATACACACAATT+CGG + chr7.2:33573180-33573199 MS.gene068998:CDS 35.0%
AATAGGAAAGAGACTCCAAA+TGG - chr7.2:33573216-33573235 None:intergenic 35.0%
ACAACAATATTCTCTCCTGA+AGG + chr7.2:33572853-33572872 MS.gene068998:CDS 35.0%
ACATCCTAATCAATACTGCT+AGG + chr7.2:33573079-33573098 MS.gene068998:CDS 35.0%
ACTACAAGGATGATATCACA+AGG + chr7.2:33573361-33573380 MS.gene068998:CDS 35.0%
CAGGAGAGAATATTGTTGTA+CGG - chr7.2:33572852-33572871 None:intergenic 35.0%
CTACAAGGATGATATCACAA+GGG + chr7.2:33573362-33573381 MS.gene068998:CDS 35.0%
TAGCTTAGTCAAATTCTCTG+AGG - chr7.2:33573517-33573536 None:intergenic 35.0%
TAGGAATCTTGTCAAAAGAC+GGG + chr7.2:33572932-33572951 MS.gene068998:CDS 35.0%
! CATTTTCTCAGTTGAAGTTG+AGG - chr7.2:33573004-33573023 None:intergenic 35.0%
! TGATACTTGACTTTTCCAGA+GGG - chr7.2:33573486-33573505 None:intergenic 35.0%
! TTGATACTTGACTTTTCCAG+AGG - chr7.2:33573487-33573506 None:intergenic 35.0%
!! AGCACAGTCAATTCTAAAAC+AGG + chr7.2:33573338-33573357 MS.gene068998:CDS 35.0%
!! AGCAGTATTGATTAGGATGT+TGG - chr7.2:33573079-33573098 None:intergenic 35.0%
!! GAAGGTTCTGAGTAAAACTA+TGG + chr7.2:33573407-33573426 MS.gene068998:CDS 35.0%
ACTAAGCTATTGGTGAAGTC+TGG + chr7.2:33573528-33573547 MS.gene068998:CDS 40.0%
AGCAGTTCAACTTGCATTGA+AGG + chr7.2:33573389-33573408 MS.gene068998:CDS 40.0%
AGGAATCTTGTCAAAAGACG+GGG + chr7.2:33572933-33572952 MS.gene068998:CDS 40.0%
CTAAGCTATTGGTGAAGTCT+GGG + chr7.2:33573529-33573548 MS.gene068998:CDS 40.0%
CTTTACATGAGTGATCCAAG+CGG + chr7.2:33573267-33573286 MS.gene068998:CDS 40.0%
TAAGCTATTGGTGAAGTCTG+GGG + chr7.2:33573530-33573549 MS.gene068998:CDS 40.0%
TCTCTCTGTGATACTAAGCA+AGG + chr7.2:33573165-33573184 MS.gene068998:CDS 40.0%
! GAAGTTTTTCTTTCACCCTC+TGG + chr7.2:33573468-33573487 MS.gene068998:CDS 40.0%
! TGCAAGTTCAAGCTTTTCTG+AGG - chr7.2:33573448-33573467 None:intergenic 40.0%
!! GGATCCTAGCAGTATTGATT+AGG - chr7.2:33573086-33573105 None:intergenic 40.0%
!! TGTTCTGGTTGGTGAAAAGA+AGG + chr7.2:33572957-33572976 MS.gene068998:CDS 40.0%
AACTATGGTGGTTGGAAAGC+TGG + chr7.2:33573291-33573310 MS.gene068998:CDS 45.0%
AGTGATCCAAGCGGAAACTA+TGG + chr7.2:33573276-33573295 MS.gene068998:CDS 45.0%
CAAAGGAGTAACGTTGTGCT+TGG - chr7.2:33573107-33573126 None:intergenic 45.0%
CAAGGATACACACAATTCGG+TGG + chr7.2:33573183-33573202 MS.gene068998:CDS 45.0%
CTCTTTCCTATTTGCAGGCT+GGG + chr7.2:33573224-33573243 MS.gene068998:CDS 45.0%
GATTGAACCAACTGCTCAAC+AGG - chr7.2:33573147-33573166 None:intergenic 45.0%
GCAGGCTGGGACAAAAATTT+CGG + chr7.2:33573237-33573256 MS.gene068998:CDS 45.0%
GGAAAGAGACTCCAAATGGT+CGG - chr7.2:33573212-33573231 None:intergenic 45.0%
GGAAATGCTGGTACTGCAAT+AGG + chr7.2:33572913-33572932 MS.gene068998:CDS 45.0%
GGAGTCTCTTTCCTATTTGC+AGG + chr7.2:33573219-33573238 MS.gene068998:CDS 45.0%
GTACCAAGTGGAGTATGCAA+TGG + chr7.2:33572882-33572901 MS.gene068998:CDS 45.0%
TCTCTTTCCTATTTGCAGGC+TGG + chr7.2:33573223-33573242 MS.gene068998:CDS 45.0%
TTAGAATTGACTGTGCTGCC+TGG - chr7.2:33573335-33573354 None:intergenic 45.0%
! AGTTGAGGTTTGAAGCAGCT+TGG - chr7.2:33572989-33573008 None:intergenic 45.0%
! TTTTGTCCCAGCCTGCAAAT+AGG - chr7.2:33573233-33573252 None:intergenic 45.0%
!! CATTGGCTCTTGGTAAGCAA+AGG - chr7.2:33573124-33573143 None:intergenic 45.0%
AAAGACGGGGTTGTTCTGGT+TGG + chr7.2:33572946-33572965 MS.gene068998:CDS 50.0%
AAGCAGTGTCTGTTGCTGGT+TGG - chr7.2:33573560-33573579 None:intergenic 50.0%
ACCAACTGCTCAACAGGCAT+TGG - chr7.2:33573141-33573160 None:intergenic 50.0%
ACTTGGTACAGACGTCCTTC+AGG - chr7.2:33572871-33572890 None:intergenic 50.0%
ATGTAGCATGTGCTGTCGCT+GGG + chr7.2:33573040-33573059 MS.gene068998:CDS 50.0%
CAACCACCATAGTTTCCGCT+TGG - chr7.2:33573285-33573304 None:intergenic 50.0%
CAAGCGGAAACTATGGTGGT+TGG + chr7.2:33573283-33573302 MS.gene068998:CDS 50.0%
CCAAGTGGAGTATGCAATGG+AGG + chr7.2:33572885-33572904 MS.gene068998:CDS 50.0%
CCTCCATTGCATACTCCACT+TGG - chr7.2:33572888-33572907 None:intergenic 50.0%
GAGTATGCAATGGAGGCCAT+CGG + chr7.2:33572892-33572911 MS.gene068998:CDS 50.0%
GATCCAAGCGGAAACTATGG+TGG + chr7.2:33573279-33573298 MS.gene068998:CDS 50.0%
GTCAAAAGACGGGGTTGTTC+TGG + chr7.2:33572942-33572961 MS.gene068998:CDS 50.0%
TGAAGGACGTCTGTACCAAG+TGG + chr7.2:33572870-33572889 MS.gene068998:CDS 50.0%
TGCAGTACCAGCATTTCCGA+TGG - chr7.2:33572911-33572930 None:intergenic 50.0%
! AGCAGTGTCTGTTGCTGGTT+GGG - chr7.2:33573559-33573578 None:intergenic 50.0%
!! GGTTGGAAAGCTGGTGCTAT+TGG + chr7.2:33573300-33573319 MS.gene068998:CDS 50.0%
!! TGCTATTGGTGCCAACAACC+AGG + chr7.2:33573314-33573333 MS.gene068998:CDS 50.0%
ATGGAGGCCATCGGAAATGC+TGG + chr7.2:33572901-33572920 MS.gene068998:CDS 55.0%
CATGTAGCATGTGCTGTCGC+TGG + chr7.2:33573039-33573058 MS.gene068998:CDS 55.0%
GCCAATGCCTGTTGAGCAGT+TGG + chr7.2:33573137-33573156 MS.gene068998:CDS 55.0%
TGACTGTGCTGCCTGGTTGT+TGG - chr7.2:33573328-33573347 None:intergenic 55.0%
TTCGGTGGTCTCCGACCATT+TGG + chr7.2:33573198-33573217 MS.gene068998:CDS 55.0%
!! GCTCAACAGGCATTGGCTCT+TGG - chr7.2:33573134-33573153 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 33572829 33573581 33572829 ID=MS.gene068998
chr7.2 mRNA 33572829 33573581 33572829 ID=MS.gene068998.t1;Parent=MS.gene068998
chr7.2 exon 33572829 33573581 33572829 ID=MS.gene068998.t1.exon1;Parent=MS.gene068998.t1
chr7.2 CDS 33572829 33573581 33572829 ID=cds.MS.gene068998.t1;Parent=MS.gene068998.t1
Gene Sequence

>MS.gene068998

ATGTCTCGAAGATATGATAGCCGTACAACAATATTCTCTCCTGAAGGACGTCTGTACCAAGTGGAGTATGCAATGGAGGCCATCGGAAATGCTGGTACTGCAATAGGAATCTTGTCAAAAGACGGGGTTGTTCTGGTTGGTGAAAAGAAGGTGACATCCAAGCTGCTTCAAACCTCAACTTCAACTGAGAAAATGTACAAGATCGATGATCATGTAGCATGTGCTGTCGCTGGGATTATGTCTGACGCCAACATCCTAATCAATACTGCTAGGATCCAAGCACAACGTTACTCCTTTGCTTACCAAGAGCCAATGCCTGTTGAGCAGTTGGTTCAATCTCTCTGTGATACTAAGCAAGGATACACACAATTCGGTGGTCTCCGACCATTTGGAGTCTCTTTCCTATTTGCAGGCTGGGACAAAAATTTCGGCTTTCAACTTTACATGAGTGATCCAAGCGGAAACTATGGTGGTTGGAAAGCTGGTGCTATTGGTGCCAACAACCAGGCAGCACAGTCAATTCTAAAACAGGACTACAAGGATGATATCACAAGGGAAGAAGCAGTTCAACTTGCATTGAAGGTTCTGAGTAAAACTATGGACAGCACTAGTCTTACCTCAGAAAAGCTTGAACTTGCAGAAGTTTTTCTTTCACCCTCTGGAAAAGTCAAGTATCAAGTTTGCTCCTCAGAGAATTTGACTAAGCTATTGGTGAAGTCTGGGGTGACCCAACCAGCAACAGACACTGCTTAG
Protein sequence

>MS.gene068998.t1

MSRRYDSRTTIFSPEGRLYQVEYAMEAIGNAGTAIGILSKDGVVLVGEKKVTSKLLQTSTSTEKMYKIDDHVACAVAGIMSDANILINTARIQAQRYSFAYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNFGFQLYMSDPSGNYGGWKAGAIGANNQAAQSILKQDYKDDITREEAVQLALKVLSKTMDSTSLTSEKLELAEVFLSPSGKVKYQVCSSENLTKLLVKSGVTQPATDTA