AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024828

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024828.t1 MTR_5g006840 100.000 203 0 0 1 203 1 203 5.80e-153 422
MS.gene024828.t1 MTR_5g012470 96.000 200 8 0 1 200 1 200 1.27e-145 403
MS.gene024828.t1 MTR_2g050000 95.413 109 3 1 95 203 2 108 1.31e-71 213
MS.gene024828.t1 MTR_1g112250 30.464 151 94 3 13 155 45 192 8.39e-13 65.5
MS.gene024828.t1 MTR_3g109210 26.087 161 117 1 3 163 15 173 1.72e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024828.t1 AT3G22630 87.129 202 26 0 1 202 1 202 6.55e-135 376
MS.gene024828.t1 AT4G14800 87.374 198 25 0 1 198 1 198 7.00e-132 369
MS.gene024828.t1 AT4G14800 80.841 214 25 1 1 198 1 214 2.21e-127 358
MS.gene024828.t1 AT3G14290 31.126 151 93 3 13 155 45 192 3.05e-13 66.6

Find 41 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGTTGATAAGTGCATTT+TGG 0.220132 5.4:+2449116 MS.gene024828:CDS
CTTTGTATCAATTCCGTAAT+GGG 0.276520 5.4:+2445843 MS.gene024828:CDS
AAGCTTGAAAAGGGAGCTTT+TGG 0.328358 5.4:+2449018 MS.gene024828:CDS
TCTGTGAACATCCTTCTTGC+TGG 0.377484 5.4:+2448937 MS.gene024828:CDS
GCTTTGTATCAATTCCGTAA+TGG 0.426748 5.4:+2445842 MS.gene024828:CDS
AGAAGGATGTTCACAGAGTA+GGG 0.434546 5.4:-2448931 None:intergenic
GACACTTCACAAGCTTGAAA+AGG 0.445538 5.4:+2449008 MS.gene024828:CDS
CTAGCGGTGAACCCGGTGAC+AGG 0.462278 5.4:+2445699 MS.gene024828:CDS
AAGAAGGATGTTCACAGAGT+AGG 0.469415 5.4:-2448932 None:intergenic
GAACATGATCTTGTCTTCGT+TGG 0.478439 5.4:-2445651 None:intergenic
TGGTGAGAGGAATCCCATTA+CGG 0.502878 5.4:-2445856 None:intergenic
TTTGGAAATCAGATCAAGGT+TGG 0.503786 5.4:+2449134 MS.gene024828:CDS
GGATTAAATCAAGCTGAAGC+AGG 0.515437 5.4:-2449243 None:intergenic
ACACTTCACAAGCTTGAAAA+GGG 0.519514 5.4:+2449009 MS.gene024828:CDS
AATCAGATCAAGGTTGGTTG+TGG 0.521276 5.4:+2449140 MS.gene024828:CDS
TGGTAATGGCTTCGCGATCG+TGG 0.521317 5.4:+2445586 MS.gene024828:CDS
GCTTGCCACCGCTCTTCGCA+AGG 0.527948 5.4:+2445910 MS.gene024828:CDS
CTTCCACGTTCATTCCGCTA+TGG 0.534157 5.4:-2449083 None:intergenic
GCTGGTTATGACAAAGAGAC+AGG 0.546617 5.4:+2448955 MS.gene024828:CDS
TAGTCAATGTAATATAGTGA+TGG 0.549687 5.4:-2448979 None:intergenic
TAATCCGAAAACGCATTCCA+TGG 0.555612 5.4:-2445561 None:intergenic
ATCGCTGCTAGCGGTGAACC+CGG 0.569631 5.4:+2445692 MS.gene024828:CDS
CTTTGTCATAACCAGCAAGA+AGG 0.572175 5.4:-2448948 None:intergenic
AAGTGAGTGAAACCTGTCAC+CGG 0.573344 5.4:-2445711 None:intergenic
GCGGCAGCAGCTGTGGTGAG+AGG 0.575158 5.4:-2445869 None:intergenic
AGTCAATGTAATATAGTGAT+GGG 0.575452 5.4:-2448978 None:intergenic
GAAATTAGCGGCAGCAGCTG+TGG 0.584921 5.4:-2445876 None:intergenic
CGCATGGCGCGAATCAGTCA+AGG 0.602986 5.4:+2449215 MS.gene024828:CDS
CTCGCCACGAGTGAAATTAG+CGG 0.612760 5.4:-2445888 None:intergenic
GAATACCTTGCGAAGAGCGG+TGG 0.614639 5.4:-2445915 None:intergenic
CTTCGTTGGATTTGTGAACG+AGG 0.622483 5.4:-2445637 None:intergenic
CTTCCATAGCGGAATGAACG+TGG 0.622934 5.4:+2449080 MS.gene024828:CDS
CACAAACTCATCGCTGCTAG+CGG 0.629383 5.4:+2445683 MS.gene024828:CDS
ATGGTGCAAGAGAGTACGCA+TGG 0.636054 5.4:+2449199 MS.gene024828:CDS
ATTGAATACCTTGCGAAGAG+CGG 0.643228 5.4:-2445918 None:intergenic
GCTGCCGCTAATTTCACTCG+TGG 0.645223 5.4:+2445884 MS.gene024828:CDS
CGATGAGTTTGTGTGAATCG+AGG 0.648197 5.4:-2445673 None:intergenic
AGTGAGTGAAACCTGTCACC+GGG 0.655029 5.4:-2445710 None:intergenic
GTGAACGAGGATGCTGTGAA+CGG 0.695181 5.4:-2445624 None:intergenic
TAATGGCTTCGCGATCGTGG+TGG 0.707184 5.4:+2445589 MS.gene024828:CDS
ATGGACAGACACTTCCATAG+CGG 0.741310 5.4:+2449069 MS.gene024828:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGAAAAAAAATAAAAAG+GGG - chr5.4:2447017-2447036 None:intergenic 10.0%
!!! AATCTTTTTATTGATAATAA+AGG - chr5.4:2448649-2448668 None:intergenic 10.0%
!!! ATTAAAATAGTCATAATTTT+GGG - chr5.4:2447778-2447797 None:intergenic 10.0%
!!! TTATCAATAAAAAGATTTTT+TGG + chr5.4:2448652-2448671 MS.gene024828:intron 10.0%
!! AAAATTAGGAAAACAAAAAA+AGG - chr5.4:2445956-2445975 None:intergenic 15.0%
!! AAATTAGGAAAACAAAAAAA+GGG - chr5.4:2445955-2445974 None:intergenic 15.0%
!! AATGTAAAGTGATTTATATT+TGG - chr5.4:2447625-2447644 None:intergenic 15.0%
!!! AATAAAAAGATTTTTTGGAA+AGG + chr5.4:2448657-2448676 MS.gene024828:intron 15.0%
!!! ACAATTTTTATAACAAAGTT+TGG - chr5.4:2449171-2449190 None:intergenic 15.0%
!!! ATTAGGGTTTTAAAAAATTA+AGG - chr5.4:2447055-2447074 None:intergenic 15.0%
!!! CAAAATTATGACTATTTTAA+TGG + chr5.4:2447777-2447796 MS.gene024828:intron 15.0%
!!! CATTAAAATAGTCATAATTT+TGG - chr5.4:2447779-2447798 None:intergenic 15.0%
!!! TATGTTATTTATCAATAAGT+AGG + chr5.4:2448367-2448386 MS.gene024828:intron 15.0%
!!! TGTATTTTTTATTTCACTTT+TGG + chr5.4:2446085-2446104 MS.gene024828:intron 15.0%
!!! TTACTAGTACTATATAATTT+TGG + chr5.4:2447846-2447865 MS.gene024828:intron 15.0%
!! AACAACAAAAAAAAAAACCA+AGG - chr5.4:2447824-2447843 None:intergenic 20.0%
!! AATATACACAACCAATAATA+CGG - chr5.4:2447425-2447444 None:intergenic 20.0%
!! AATTAGGAAAACAAAAAAAG+GGG - chr5.4:2445954-2445973 None:intergenic 20.0%
!! CTATTCAACACTAAATATTA+GGG - chr5.4:2445985-2446004 None:intergenic 20.0%
!! CTCATTTGTCTTAATTTATA+TGG + chr5.4:2448564-2448583 MS.gene024828:intron 20.0%
!! TAGATAATGATAGAACATTA+TGG - chr5.4:2447176-2447195 None:intergenic 20.0%
!! TATTAGGGTTTGAAAAAATT+AGG - chr5.4:2445970-2445989 None:intergenic 20.0%
!! TCATACATATATCTATACAA+CGG + chr5.4:2447096-2447115 MS.gene024828:intron 20.0%
!! TCTATTCAACACTAAATATT+AGG - chr5.4:2445986-2446005 None:intergenic 20.0%
!! TGTTATAAAAATTGTTGACA+AGG + chr5.4:2449176-2449195 MS.gene024828:CDS 20.0%
!!! AACACTAATCATCTTTTTAT+AGG + chr5.4:2448588-2448607 MS.gene024828:intron 20.0%
!!! ATAATTGATTTTGACATGTT+TGG + chr5.4:2447487-2447506 MS.gene024828:intron 20.0%
!!! ATATTTGGTTTTACTTTTAG+AGG + chr5.4:2447440-2447459 MS.gene024828:intron 20.0%
!!! ATTTTTATAACAAAGTTTGG+AGG - chr5.4:2449168-2449187 None:intergenic 20.0%
!!! ATTTTTTATTTCACTTTTGG+AGG + chr5.4:2446088-2446107 MS.gene024828:intron 20.0%
!!! GTAATTTTCCTTTGTTTTTT+TGG - chr5.4:2448206-2448225 None:intergenic 20.0%
!!! TATTATTGGTTGTGTATATT+TGG + chr5.4:2447425-2447444 MS.gene024828:intron 20.0%
! AAATACTGGATATATATGCT+TGG - chr5.4:2447967-2447986 None:intergenic 25.0%
! AAATAGGGTTTGAATGAAAT+GGG + chr5.4:2448329-2448348 MS.gene024828:intron 25.0%
! AAATATTACTAGTACTACCT+TGG + chr5.4:2447804-2447823 MS.gene024828:intron 25.0%
! AATACTGGATATATATGCTT+GGG - chr5.4:2447966-2447985 None:intergenic 25.0%
! AATCCATGAAAAGTTTAGAT+GGG + chr5.4:2446304-2446323 MS.gene024828:intron 25.0%
! ACATACATAAACAATAATGC+AGG - chr5.4:2446398-2446417 None:intergenic 25.0%
! AGTCAATGTAATATAGTGAT+GGG - chr5.4:2448981-2449000 None:intergenic 25.0%
! ATAAAAATTGTTGACAAGGA+TGG + chr5.4:2449180-2449199 MS.gene024828:CDS 25.0%
! ATAAATTAAGACAAATGAGC+AGG - chr5.4:2448563-2448582 None:intergenic 25.0%
! ATATACACAACCAATAATAC+GGG - chr5.4:2447424-2447443 None:intergenic 25.0%
! ATTAGGAAAACAAAAAAAGG+GGG - chr5.4:2445953-2445972 None:intergenic 25.0%
! GTATAGATATATGTATGATG+AGG - chr5.4:2447094-2447113 None:intergenic 25.0%
! TAGTCAATGTAATATAGTGA+TGG - chr5.4:2448982-2449001 None:intergenic 25.0%
! TATTAAATACAATAAGTCGC+TGG - chr5.4:2446622-2446641 None:intergenic 25.0%
! TCTATAATAACAATGCAACA+TGG - chr5.4:2448870-2448889 None:intergenic 25.0%
!! AATTCTTTTCGACAATTCTA+CGG - chr5.4:2448782-2448801 None:intergenic 25.0%
!! AATTGATTCTAGCTAAAAGT+CGG - chr5.4:2447651-2447670 None:intergenic 25.0%
!! AGCATATATATCCAGTATTT+TGG + chr5.4:2447967-2447986 MS.gene024828:intron 25.0%
!! GGATTTAGTTTATGTCAATT+GGG + chr5.4:2447259-2447278 MS.gene024828:intron 25.0%
!! TGGATTTAGTTTATGTCAAT+TGG + chr5.4:2447258-2447277 MS.gene024828:intron 25.0%
!! TTTGAGATTTGACAAGTATT+GGG + chr5.4:2446750-2446769 MS.gene024828:intron 25.0%
!!! AAGGATAGTACTGATTATTA+GGG - chr5.4:2447071-2447090 None:intergenic 25.0%
!!! AGATTTCAAGTAGTAGTTTT+CGG - chr5.4:2446905-2446924 None:intergenic 25.0%
!!! ATAATTGATTTTGACGTGTT+TGG + chr5.4:2446135-2446154 MS.gene024828:intron 25.0%
!!! ATTCCTTTTTTTCACTTTTC+AGG + chr5.4:2445786-2445805 MS.gene024828:intron 25.0%
!!! CATCATTGATAGTGAAAAAT+AGG - chr5.4:2449052-2449071 None:intergenic 25.0%
!!! CTATTTTTCACTATCAATGA+TGG + chr5.4:2449050-2449069 MS.gene024828:CDS 25.0%
!!! GATTTTAGCCTTGAATTTAA+TGG + chr5.4:2447574-2447593 MS.gene024828:intron 25.0%
!!! GTACTATATAATTTTGGTCT+AGG + chr5.4:2447852-2447871 MS.gene024828:intron 25.0%
!!! TAATTGATTTTGACGTGTTT+GGG + chr5.4:2446136-2446155 MS.gene024828:intron 25.0%
!!! TTCCTTTTTTTCACTTTTCA+GGG + chr5.4:2445787-2445806 MS.gene024828:intron 25.0%
!!! TTTTGAGATTTGACAAGTAT+TGG + chr5.4:2446749-2446768 MS.gene024828:intron 25.0%
!!! TTTTTTTGGAGTGATGATTA+TGG - chr5.4:2448192-2448211 None:intergenic 25.0%
AAAAAGGGGTAATTTGAACT+TGG - chr5.4:2447003-2447022 None:intergenic 30.0%
AAACACATGACAAGTTTAGA+TGG + chr5.4:2447331-2447350 MS.gene024828:intron 30.0%
AACACATGACAAGTTTAGAT+GGG + chr5.4:2447332-2447351 MS.gene024828:intron 30.0%
AACCCTGAAAAGTGAAAAAA+AGG - chr5.4:2445792-2445811 None:intergenic 30.0%
AACCTATGTCAAAAATCTCT+TGG + chr5.4:2446697-2446716 MS.gene024828:intron 30.0%
AAGGGTTTGAAAAAGTTTAC+AGG - chr5.4:2446651-2446670 None:intergenic 30.0%
AAGTTGAATCTCTTTCTTTC+TGG + chr5.4:2447725-2447744 MS.gene024828:intron 30.0%
AATCTAAGCATATCAATAGC+TGG - chr5.4:2448821-2448840 None:intergenic 30.0%
ACTTGCAAAAATGGTTGAAT+GGG - chr5.4:2448262-2448281 None:intergenic 30.0%
AGATATATGTATGATGAGGA+AGG - chr5.4:2447090-2447109 None:intergenic 30.0%
AGTTTATTCACACGTGAATT+TGG + chr5.4:2448895-2448914 MS.gene024828:intron 30.0%
ATATATGCTTGGGTTCATAT+GGG - chr5.4:2447956-2447975 None:intergenic 30.0%
ATCCATGAAAAGTTTAGATG+GGG + chr5.4:2446305-2446324 MS.gene024828:intron 30.0%
CAGTTGAAGATGAAATAAAG+AGG + chr5.4:2446814-2446833 MS.gene024828:intron 30.0%
CTTTGTATCAATTCCGTAAT+GGG + chr5.4:2445843-2445862 MS.gene024828:CDS 30.0%
GAAATAGGGTTTGAATGAAA+TGG + chr5.4:2448328-2448347 MS.gene024828:intron 30.0%
GAATACATTTGTGCTAAAGT+GGG - chr5.4:2446252-2446271 None:intergenic 30.0%
GAATCCATGAAAAGTTTAGA+TGG + chr5.4:2446303-2446322 MS.gene024828:intron 30.0%
GGATACATTTGTGTAAAAGT+GGG - chr5.4:2447604-2447623 None:intergenic 30.0%
TATATATGCTTGGGTTCATA+TGG - chr5.4:2447957-2447976 None:intergenic 30.0%
TATTTCTCTCAAAATTTGGG+GGG - chr5.4:2448314-2448333 None:intergenic 30.0%
TCATCACTCCAAAAAAACAA+AGG + chr5.4:2448195-2448214 MS.gene024828:intron 30.0%
TGAATACATTTGTGCTAAAG+TGG - chr5.4:2446253-2446272 None:intergenic 30.0%
TGGATACATTTGTGTAAAAG+TGG - chr5.4:2447605-2447624 None:intergenic 30.0%
! ACCCTATTTCTCTCAAAATT+TGG - chr5.4:2448318-2448337 None:intergenic 30.0%
! CCCTATTTCTCTCAAAATTT+GGG - chr5.4:2448317-2448336 None:intergenic 30.0%
! CCTATTTCTCTCAAAATTTG+GGG - chr5.4:2448316-2448335 None:intergenic 30.0%
! CTATTTCTCTCAAAATTTGG+GGG - chr5.4:2448315-2448334 None:intergenic 30.0%
! CTCTTTATTTCATCTTCAAC+TGG - chr5.4:2446816-2446835 None:intergenic 30.0%
! TACCAAGAGATTTTTGACAT+AGG - chr5.4:2446702-2446721 None:intergenic 30.0%
! TTGAGATTTGACAAGTATTG+GGG + chr5.4:2446751-2446770 MS.gene024828:intron 30.0%
!! CCCAAATTTTGAGAGAAATA+GGG + chr5.4:2448314-2448333 MS.gene024828:intron 30.0%
!! GAAGGATAGTACTGATTATT+AGG - chr5.4:2447072-2447091 None:intergenic 30.0%
!! TACACCTCTAGAATCAAATT+TGG - chr5.4:2447468-2447487 None:intergenic 30.0%
!! TCTTGTTGATAAGTGCATTT+TGG + chr5.4:2449116-2449135 MS.gene024828:CDS 30.0%
!! TGTTTCTATCTCCTTTTGAA+CGG - chr5.4:2447316-2447335 None:intergenic 30.0%
!!! ATATCCAGTATTTTGGTTGA+GGG + chr5.4:2447974-2447993 MS.gene024828:intron 30.0%
!!! CATTAGATTTTTTACGCTAC+AGG + chr5.4:2448054-2448073 MS.gene024828:intron 30.0%
!!! TATATCCAGTATTTTGGTTG+AGG + chr5.4:2447973-2447992 MS.gene024828:intron 30.0%
AAAGCTACAAATCCTAGCTT+CGG - chr5.4:2446188-2446207 None:intergenic 35.0%
AAATAGGTTGTGGCTAATGA+AGG - chr5.4:2448166-2448185 None:intergenic 35.0%
AAGGGTTATCTCTCATACAA+TGG - chr5.4:2446854-2446873 None:intergenic 35.0%
AATAGGTTGTGGCTAATGAA+GGG - chr5.4:2448165-2448184 None:intergenic 35.0%
AATCTTGAAGCTTAGTGACA+GGG + chr5.4:2446721-2446740 MS.gene024828:intron 35.0%
ACAAATGAGCAGGAACTATT+AGG - chr5.4:2448553-2448572 None:intergenic 35.0%
ACACTTATGACTTACCTATC+CGG + chr5.4:2448748-2448767 MS.gene024828:intron 35.0%
ACACTTCACAAGCTTGAAAA+GGG + chr5.4:2449009-2449028 MS.gene024828:CDS 35.0%
AGAGATTCACAAACTGAAGT+AGG - chr5.4:2446789-2446808 None:intergenic 35.0%
AGGAATCTTAAAGGACTTTC+AGG + chr5.4:2448021-2448040 MS.gene024828:intron 35.0%
AGTCTGAGCCAAACTTAATA+AGG + chr5.4:2448279-2448298 MS.gene024828:intron 35.0%
ATGAGTTCGTTTGATCCATA+TGG + chr5.4:2446029-2446048 MS.gene024828:intron 35.0%
CAATTCTACGGATCAATATC+CGG - chr5.4:2448770-2448789 None:intergenic 35.0%
GACTTGCAAAAATGGTTGAA+TGG - chr5.4:2448263-2448282 None:intergenic 35.0%
GCTTTGTATCAATTCCGTAA+TGG + chr5.4:2445842-2445861 MS.gene024828:CDS 35.0%
GTAGATCAACAAGTGAAGAA+AGG + chr5.4:2446422-2446441 MS.gene024828:intron 35.0%
TAATCTTGAAGCTTAGTGAC+AGG + chr5.4:2446720-2446739 MS.gene024828:intron 35.0%
TCCCAAAACACATAAGAACA+CGG + chr5.4:2448108-2448127 MS.gene024828:intron 35.0%
TTTGGAAATCAGATCAAGGT+TGG + chr5.4:2449134-2449153 MS.gene024828:CDS 35.0%
TTTGGCATGTCATTCTACAA+GGG - chr5.4:2446872-2446891 None:intergenic 35.0%
! ACATAGGTTTTCAGTTTCCA+GGG - chr5.4:2446686-2446705 None:intergenic 35.0%
! ACCGTGTTCTTATGTGTTTT+GGG - chr5.4:2448112-2448131 None:intergenic 35.0%
! ATTATGGTGGAAATAGGTTG+TGG - chr5.4:2448176-2448195 None:intergenic 35.0%
! GTGATGATTATGGTGGAAAT+AGG - chr5.4:2448182-2448201 None:intergenic 35.0%
! TCTTTTTCTGTGTATGTGAC+TGG - chr5.4:2448700-2448719 None:intergenic 35.0%
! TGGGTTGACCATTAAATTCA+AGG - chr5.4:2447585-2447604 None:intergenic 35.0%
!! ATTGATTTTAACCCGAAGCT+AGG + chr5.4:2446173-2446192 MS.gene024828:intron 35.0%
!! CCCCAAATTTTGAGAGAAAT+AGG + chr5.4:2448313-2448332 MS.gene024828:intron 35.0%
!! CTAAGTTAAGTTGATGATGG+AGG - chr5.4:2448141-2448160 None:intergenic 35.0%
!! GCATTTTGGAAATCAGATCA+AGG + chr5.4:2449130-2449149 MS.gene024828:CDS 35.0%
!! GGACTAAGTTAAGTTGATGA+TGG - chr5.4:2448144-2448163 None:intergenic 35.0%
!!! GTTTTCGGATTAGTTGGTAA+TGG + chr5.4:2445572-2445591 MS.gene024828:CDS 35.0%
!!! TATTTTGGTTGAGGGAAGTT+TGG + chr5.4:2447982-2448001 MS.gene024828:intron 35.0%
!!! TGCATTTTTTTTCCCCATAC+TGG + chr5.4:2447200-2447219 MS.gene024828:intron 35.0%
!!! TTTTGGAGTGATGATTATGG+TGG - chr5.4:2448189-2448208 None:intergenic 35.0%
AAACTTTGGATGGGAAGAGA+TGG + chr5.4:2446953-2446972 MS.gene024828:intron 40.0%
AACTTTGGATGGGAAGAGAT+GGG + chr5.4:2446954-2446973 MS.gene024828:intron 40.0%
AAGAAGGATGTTCACAGAGT+AGG - chr5.4:2448935-2448954 None:intergenic 40.0%
AAGCTACAAATCCTAGCTTC+GGG - chr5.4:2446187-2446206 None:intergenic 40.0%
AATGAACAGATCACAGATGG+TGG - chr5.4:2448233-2448252 None:intergenic 40.0%
ACACAACCAATAATACGGGA+AGG - chr5.4:2447420-2447439 None:intergenic 40.0%
ACTTCCCTCAACCAAAATAC+TGG - chr5.4:2447981-2448000 None:intergenic 40.0%
ACTTTCATGTTAGTTCCAGC+AGG + chr5.4:2446480-2446499 MS.gene024828:intron 40.0%
AGAAGGATGTTCACAGAGTA+GGG - chr5.4:2448934-2448953 None:intergenic 40.0%
AGAATCCCTTCCCGTATTAT+TGG + chr5.4:2447411-2447430 MS.gene024828:intron 40.0%
ATCAAACAAACTCTGCCAGT+TGG + chr5.4:2446509-2446528 MS.gene024828:intron 40.0%
ATCAACCCATGAAACCAGTA+TGG - chr5.4:2447217-2447236 None:intergenic 40.0%
ATCTCTTCCCATCCAAAGTT+TGG - chr5.4:2446954-2446973 None:intergenic 40.0%
ATTGAATACCTTGCGAAGAG+CGG - chr5.4:2445921-2445940 None:intergenic 40.0%
CACAACCAATAATACGGGAA+GGG - chr5.4:2447419-2447438 None:intergenic 40.0%
CAGCTAACCAAACTTTGGAT+GGG + chr5.4:2446944-2446963 MS.gene024828:intron 40.0%
CTTTGGCATGTCATTCTACA+AGG - chr5.4:2446873-2446892 None:intergenic 40.0%
CTTTGTCATAACCAGCAAGA+AGG - chr5.4:2448951-2448970 None:intergenic 40.0%
GAACATGATCTTGTCTTCGT+TGG - chr5.4:2445654-2445673 None:intergenic 40.0%
GACACTTCACAAGCTTGAAA+AGG + chr5.4:2449008-2449027 MS.gene024828:CDS 40.0%
GATTCTCTCAAGTGTGATTC+TGG - chr5.4:2446288-2446307 None:intergenic 40.0%
GGAGCCAAATTTGATTCTAG+AGG + chr5.4:2447461-2447480 MS.gene024828:intron 40.0%
GGTCTGTTCAGGAATCTTAA+AGG + chr5.4:2448012-2448031 MS.gene024828:intron 40.0%
GTCAATGAACAGATCACAGA+TGG - chr5.4:2448236-2448255 None:intergenic 40.0%
TAACCCAGCTAACCAAACTT+TGG + chr5.4:2446939-2446958 MS.gene024828:intron 40.0%
TAGTTGACATGCGAATGGTT+TGG + chr5.4:2446532-2446551 MS.gene024828:intron 40.0%
TCAACCCATGAAACCAGTAT+GGG - chr5.4:2447216-2447235 None:intergenic 40.0%
TTAAGATTCCTGAACAGACC+AGG - chr5.4:2448012-2448031 None:intergenic 40.0%
TTCAGTTTCCAGGGTTATTG+AGG - chr5.4:2446677-2446696 None:intergenic 40.0%
TTGGCTCAGACTTGCAAAAA+TGG - chr5.4:2448271-2448290 None:intergenic 40.0%
TTGTTTGATATGGAACCTGC+TGG - chr5.4:2446498-2446517 None:intergenic 40.0%
! AAGCTTGAAAAGGGAGCTTT+TGG + chr5.4:2449018-2449037 MS.gene024828:CDS 40.0%
! AATCAGATCAAGGTTGGTTG+TGG + chr5.4:2449140-2449159 MS.gene024828:CDS 40.0%
! CACCCCATCTAAACTTTTCA+TGG - chr5.4:2446310-2446329 None:intergenic 40.0%
! CACCGTGTTCTTATGTGTTT+TGG - chr5.4:2448113-2448132 None:intergenic 40.0%
! CATCCAAAGTTTGGTTAGCT+GGG - chr5.4:2446945-2446964 None:intergenic 40.0%
! CTGGCAGAGTTTGTTTGATA+TGG - chr5.4:2446508-2446527 None:intergenic 40.0%
! CTTTCTTTTACTGTCAGTGC+TGG + chr5.4:2448621-2448640 MS.gene024828:intron 40.0%
! GACATAGGTTTTCAGTTTCC+AGG - chr5.4:2446687-2446706 None:intergenic 40.0%
! TGAATCTCTTTCTTTCTGGC+TGG + chr5.4:2447729-2447748 MS.gene024828:intron 40.0%
!! ACCTGCAGCCTTATTAAGTT+TGG - chr5.4:2448290-2448309 None:intergenic 40.0%
!! GAAAAGGGAGCTTTTGGTTA+TGG + chr5.4:2449024-2449043 MS.gene024828:CDS 40.0%
!! TTGACAAGTATTGGGGTTAG+TGG + chr5.4:2446758-2446777 MS.gene024828:intron 40.0%
!! TTTCCCCATACTGGTTTCAT+GGG + chr5.4:2447209-2447228 MS.gene024828:intron 40.0%
!!! GAATGCGTTTTCGGATTAGT+TGG + chr5.4:2445566-2445585 MS.gene024828:CDS 40.0%
!!! TTTTCCCCATACTGGTTTCA+TGG + chr5.4:2447208-2447227 MS.gene024828:intron 40.0%
AAGCCTTAACCCACTAAGTG+GGG + chr5.4:2447127-2447146 MS.gene024828:intron 45.0%
AAGTCCTGTCTCAGTAAGTG+AGG - chr5.4:2446059-2446078 None:intergenic 45.0%
AAGTGAGTGAAACCTGTCAC+CGG - chr5.4:2445714-2445733 None:intergenic 45.0%
ATCCCTGTTATAGCCATCGT+AGG + chr5.4:2446446-2446465 MS.gene024828:intron 45.0%
ATGGACAGACACTTCCATAG+CGG + chr5.4:2449069-2449088 MS.gene024828:CDS 45.0%
CAACCCATGAAACCAGTATG+GGG - chr5.4:2447215-2447234 None:intergenic 45.0%
CAAGCCTTAACCCACTAAGT+GGG + chr5.4:2447126-2447145 MS.gene024828:intron 45.0%
CACCTACGATGGCTATAACA+GGG - chr5.4:2446451-2446470 None:intergenic 45.0%
CCAGCTAACCAAACTTTGGA+TGG + chr5.4:2446943-2446962 MS.gene024828:intron 45.0%
CCAGGGTTATTGAGGTTCAA+GGG - chr5.4:2446669-2446688 None:intergenic 45.0%
CCATCCAAAGTTTGGTTAGC+TGG - chr5.4:2446946-2446965 None:intergenic 45.0%
CGATGAGTTTGTGTGAATCG+AGG - chr5.4:2445676-2445695 None:intergenic 45.0%
CTACGGATCAATATCCGGAT+AGG - chr5.4:2448765-2448784 None:intergenic 45.0%
CTTCGTTGGATTTGTGAACG+AGG - chr5.4:2445640-2445659 None:intergenic 45.0%
GAATAGTCTAAGCGTGCGTT+TGG - chr5.4:2447703-2447722 None:intergenic 45.0%
GCCAAACTTAATAAGGCTGC+AGG + chr5.4:2448286-2448305 MS.gene024828:intron 45.0%
GCTGGTTATGACAAAGAGAC+AGG + chr5.4:2448955-2448974 MS.gene024828:CDS 45.0%
GTCATAAGTGTGCTGTGTCA+TGG - chr5.4:2448739-2448758 None:intergenic 45.0%
GTGATGTCTCACCGTTCAAA+AGG + chr5.4:2447302-2447321 MS.gene024828:intron 45.0%
TCAAGCCTTAACCCACTAAG+TGG + chr5.4:2447125-2447144 MS.gene024828:intron 45.0%
TCACCTACGATGGCTATAAC+AGG - chr5.4:2446452-2446471 None:intergenic 45.0%
TCACTTACTGAGACAGGACT+TGG + chr5.4:2446058-2446077 MS.gene024828:intron 45.0%
TCCAGGGTTATTGAGGTTCA+AGG - chr5.4:2446670-2446689 None:intergenic 45.0%
TCTGTGAACATCCTTCTTGC+TGG + chr5.4:2448937-2448956 MS.gene024828:CDS 45.0%
TGGTGAGAGGAATCCCATTA+CGG - chr5.4:2445859-2445878 None:intergenic 45.0%
TTCGCATGTCAACTACCAAC+TGG - chr5.4:2446527-2446546 None:intergenic 45.0%
! GGTGTCGAGATTGATTCTAG+AGG + chr5.4:2446109-2446128 MS.gene024828:intron 45.0%
! GTTTTCGGCTCTCTCAACTT+TGG - chr5.4:2446890-2446909 None:intergenic 45.0%
!! ATCTCTTTCTTTCTGGCTGG+AGG + chr5.4:2447732-2447751 MS.gene024828:intron 45.0%
!! GTTGGTAGTTGACATGCGAA+TGG + chr5.4:2446527-2446546 MS.gene024828:intron 45.0%
!! TTGGTTTTCCTGGTCTGTTC+AGG + chr5.4:2448001-2448020 MS.gene024828:intron 45.0%
!!! TGAGGGAAGTTTGGTTTTCC+TGG + chr5.4:2447991-2448010 MS.gene024828:intron 45.0%
!! AAATTAGAAAAAAAATAAAA+AGG - chr5.4:2447019-2447038 None:intergenic 5.0%
!! AATTAGAAAAAAAATAAAAA+GGG - chr5.4:2447018-2447037 None:intergenic 5.0%
!! TAATGTTAAAATTTAATATT+TGG + chr5.4:2447238-2447257 MS.gene024828:intron 5.0%
AGTAAGTGAGGTCGGCCATA+TGG - chr5.4:2446047-2446066 None:intergenic 50.0%
AGTGAGTGAAACCTGTCACC+GGG - chr5.4:2445713-2445732 None:intergenic 50.0%
AGTGTGCTGTGTCATGGTGA+TGG - chr5.4:2448733-2448752 None:intergenic 50.0%
ATGTATCCGACCCCACTTAG+TGG - chr5.4:2447140-2447159 None:intergenic 50.0%
CACAAACTCATCGCTGCTAG+CGG + chr5.4:2445683-2445702 MS.gene024828:CDS 50.0%
CCCTTGAACCTCAATAACCC+TGG + chr5.4:2446666-2446685 MS.gene024828:intron 50.0%
CCTGTCTCAGTAAGTGAGGT+CGG - chr5.4:2446055-2446074 None:intergenic 50.0%
CTCGCCACGAGTGAAATTAG+CGG - chr5.4:2445891-2445910 None:intergenic 50.0%
CTTAACCCACTAAGTGGGGT+CGG + chr5.4:2447131-2447150 MS.gene024828:intron 50.0%
CTTCCACGTTCATTCCGCTA+TGG - chr5.4:2449086-2449105 None:intergenic 50.0%
GGATACCATCATCTCTCGCT+CGG - chr5.4:2446982-2447001 None:intergenic 50.0%
GTGAACGAGGATGCTGTGAA+CGG - chr5.4:2445627-2445646 None:intergenic 50.0%
GTGTGCTGTGTCATGGTGAT+GGG - chr5.4:2448732-2448751 None:intergenic 50.0%
TGTATCCGACCCCACTTAGT+GGG - chr5.4:2447139-2447158 None:intergenic 50.0%
! ATGGTGCAAGAGAGTACGCA+TGG + chr5.4:2449199-2449218 MS.gene024828:CDS 50.0%
! CTTCCATAGCGGAATGAACG+TGG + chr5.4:2449080-2449099 MS.gene024828:CDS 50.0%
! TGTGCTGTGTCATGGTGATG+GGG - chr5.4:2448731-2448750 None:intergenic 50.0%
CCGACCTCACTTACTGAGAC+AGG + chr5.4:2446052-2446071 MS.gene024828:intron 55.0%
GAAATTAGCGGCAGCAGCTG+TGG - chr5.4:2445879-2445898 None:intergenic 55.0%
GAATACCTTGCGAAGAGCGG+TGG - chr5.4:2445918-2445937 None:intergenic 55.0%
GCTGCCGCTAATTTCACTCG+TGG + chr5.4:2445884-2445903 MS.gene024828:CDS 55.0%
GTGACTGCTCTCACCTACGA+TGG - chr5.4:2446462-2446481 None:intergenic 55.0%
TAATGGCTTCGCGATCGTGG+TGG + chr5.4:2445589-2445608 MS.gene024828:CDS 55.0%
!! CGACCCCACTTAGTGGGTTA+AGG - chr5.4:2447133-2447152 None:intergenic 55.0%
!! GTGCTGTGTCATGGTGATGG+GGG - chr5.4:2448730-2448749 None:intergenic 55.0%
!! TGGTAATGGCTTCGCGATCG+TGG + chr5.4:2445586-2445605 MS.gene024828:CDS 55.0%
ATCGCTGCTAGCGGTGAACC+CGG + chr5.4:2445692-2445711 MS.gene024828:CDS 60.0%
CGCATGGCGCGAATCAGTCA+AGG + chr5.4:2449215-2449234 MS.gene024828:CDS 60.0%
GGGAACCGAGCGAGAGATGA+TGG + chr5.4:2446974-2446993 MS.gene024828:intron 60.0%
CTAGCGGTGAACCCGGTGAC+AGG + chr5.4:2445699-2445718 MS.gene024828:CDS 65.0%
GCTTGCCACCGCTCTTCGCA+AGG + chr5.4:2445910-2445929 MS.gene024828:CDS 65.0%
GCGGCAGCAGCTGTGGTGAG+AGG - chr5.4:2445872-2445891 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 2445563 2449257 2445563 ID=MS.gene024828
chr5.4 mRNA 2445563 2449257 2445563 ID=MS.gene024828.t1;Parent=MS.gene024828
chr5.4 exon 2445563 2445720 2445563 ID=MS.gene024828.t1.exon1;Parent=MS.gene024828.t1
chr5.4 CDS 2445563 2445720 2445563 ID=cds.MS.gene024828.t1;Parent=MS.gene024828.t1
chr5.4 exon 2445808 2445931 2445808 ID=MS.gene024828.t1.exon2;Parent=MS.gene024828.t1
chr5.4 CDS 2445808 2445931 2445808 ID=cds.MS.gene024828.t1;Parent=MS.gene024828.t1
chr5.4 exon 2448928 2449257 2448928 ID=MS.gene024828.t1.exon3;Parent=MS.gene024828.t1
chr5.4 CDS 2448928 2449257 2448928 ID=cds.MS.gene024828.t1;Parent=MS.gene024828.t1
Gene Sequence

>MS.gene024828

ATGGAATGCGTTTTCGGATTAGTTGGTAATGGCTTCGCGATCGTGGTGGCTGATACATCTGCCGTTCACAGCATCCTCGTTCACAAATCCAACGAAGACAAGATCATGTTCCTCGATTCACACAAACTCATCGCTGCTAGCGGTGAACCCGGTGACAGGTTTCACTCACTTTATCATCTTCTTCTTTTATCTTTGTAACTGATTTTGATTTTGATTTTGATTCATTCCTTTTTTTCACTTTTCAGGGTTCAGTTTACTGAATACATTCAAAAGAACGTTGCTTTGTATCAATTCCGTAATGGGATTCCTCTCACCACAGCTGCTGCCGCTAATTTCACTCGTGGCGAGCTTGCCACCGCTCTTCGCAAGGTATTCAATTTCTAAATACCCCCTTTTTTTGTTTTCCTAATTTTTTCAAACCCTAATATTTAGTGTTGAATAGAGATATTGTATGTGATAGAATATCATGAGTTCGTTTGATCCATATGGCCGACCTCACTTACTGAGACAGGACTTGGTTGTTGTATTTTTTATTTCACTTTTGGAGGTGTCGAGATTGATTCTAGAGGTGTATAATTGATTTTGACGTGTTTGGGTGTTCTGAAGTAGAATTGATTTTAACCCGAAGCTAGGATTTGTAGCTTTTGACTTCTATAATTGATTTTAGACTTGAATTTAATGCTCAGCCCACTTTAGCACAAATGTATTCAACTCACTTTTCTCCAGAATCACACTTGAGAGAATCCATGAAAAGTTTAGATGGGGTGAATGTGAATAGTAAGAGATTACACTTTACTCTCTCATGTAAAAATCACAATTTAAAGAGTACGCTCCTGCATTATTGTTTATGTATGTGAGAGTAGATCAACAAGTGAAGAAAGGCATCCCTGTTATAGCCATCGTAGGTGAGAGCAGTCACTTTCATGTTAGTTCCAGCAGGTTCCATATCAAACAAACTCTGCCAGTTGGTAGTTGACATGCGAATGGTTTGGTTATTCAGTTTGAACAAGAATTTCTCACGTATAACAGATGCGAACACACAGTAAAATGCTCTCACCAGCGACTTATTGTATTTAATATTCAGACCTGTAAACTTTTTCAAACCCTTGAACCTCAATAACCCTGGAAACTGAAAACCTATGTCAAAAATCTCTTGGTAATCTTGAAGCTTAGTGACAGGGAGAAATTTTGAGATTTGACAAGTATTGGGGTTAGTGGAATCACCTACTTCAGTTTGTGAATCTCTATTACCAGTTGAAGATGAAATAAAGAGGAGAATCGATAAATACCATTGTATGAGAGATAACCCTTGTAGAATGACATGCCAAAGTTGAGAGAGCCGAAAACTACTACTTGAAATCTGAATTGACGAGTAGTAACCCAGCTAACCAAACTTTGGATGGGAAGAGATGGGAACCGAGCGAGAGATGATGGTATCCAAGTTCAAATTACCCCTTTTTATTTTTTTTCTAATTTTTCTTCTTGTTTACCTTAATTTTTTAAAACCCTAATAATCAGTACTATCCTTCCTCATCATACATATATCTATACAACGGCAACAATCAAGCCTTAACCCACTAAGTGGGGTCGGATACATTGATCAAATTACGCCATAATGTTCTATCATTATCTATAATTGCATTTTTTTTCCCCATACTGGTTTCATGGGTTGATCTAATGTTAAAATTTAATATTTGGATTTAGTTTATGTCAATTGGGATTTTTTGTACACAAAAGATGTGATGTCTCACCGTTCAAAAGGAGATAGAAACACATGACAAGTTTAGATGGGATGAATTTTAATAGTGAGAGATCACACTTTACTCTCACGTGAAAATTACACTTAAGAGAATCCCTTCCCGTATTATTGGTTGTGTATATTTGGTTTTACTTTTAGAGGAGCCAAATTTGATTCTAGAGGTGTATAATTGATTTTGACATGTTTGGATGTTCTCAAGTAGAATTGATTTTAATTCGAAGTTATGATTTGTAGCTTTTGACTTCTATAATTGATTTTAGCCTTGAATTTAATGGTCAACCCACTTTTACACAAATGTATCCAAATATAAATCACTTTACATTCAACCGACTTTTAGCTAGAATCAATTTTACAAAATTAATTTATGTCGCTGTAGAACCAAACGCACGCTTAGACTATTCTAAAGTTGAATCTCTTTCTTTCTGGCTGGAGGATAGATCTATAAATAAGTAACCCAAAATTATGACTATTTTAATGGATAGAAATATTACTAGTACTACCTTGGTTTTTTTTTTTGTTGTTTCTTACTAGTACTATATAATTTTGGTCTAGGTGATGAGATGAATATGAACTTGTAAGTTGTAGCTACTGCATCTCTTGTTCGTTTATGTTTGACTTACAACAGAACTGACCCATATGAACCCAAGCATATATATCCAGTATTTTGGTTGAGGGAAGTTTGGTTTTCCTGGTCTGTTCAGGAATCTTAAAGGACTTTCAGGATTATCGTATCATTAGATTTTTTACGCTACAGGTTAATGTTTTTTTTTTTTTTTAATAAAACTATCCCAAAACACATAAGAACACGGTGTGCAACCTCCATCATCAACTTAACTTAGTCCCTTCATTAGCCACAACCTATTTCCACCATAATCATCACTCCAAAAAAACAAAGGAAAATTACTGTGCCACCATCTGTGATCTGTTCATTGACTTGCCCATTCAACCATTTTTGCAAGTCTGAGCCAAACTTAATAAGGCTGCAGGTGCCCCCCAAATTTTGAGAGAAATAGGGTTTGAATGAAATGGGAATTAAACTTTTGATTATGTTATTTATCAATAAGTAGGTGTCTGAAAAATTCAACCATTTTTGCAAGTCTGAGCCAAACTTAATAAGGCTGCAGGTGCCCCCCAAATTTTGAGAGAAATAGGGTTTGAATGAAATGGGAATTAAACTTTTGATTATGTTATTTATCAATAAGTAGGTGTCTGAAAAAAATTGTGATATCCTAATAGTTCCTGCTCATTTGTCTTAATTTATATGGCAACACTAATCATCTTTTTATAGGACTTTCTCTTCTTTCTTTTACTGTCAGTGCTGGAACCTTTATTATCAATAAAAAGATTTTTTGGAAAGGAAATGACTAGTGAACTTCCAGTCACATACACAGAAAAAGAATATCGTCCCCCATCACCATGACACAGCACACTTATGACTTACCTATCCGGATATTGATCCGTAGAATTGTCGAAAAGAATTGAAATATTTTATAAATCCAGCTATTGATATGCTTAGATTTATGTTGTTTTGCAGCAATTCATTAACCATGTTGCATTGTTATTATAGATAACGAGTTTATTCACACGTGAATTTGGTTTTCTTCAGAATCCCTACTCTGTGAACATCCTTCTTGCTGGTTATGACAAAGAGACAGGCCCATCACTATATTACATTGACTACATTGCGACACTTCACAAGCTTGAAAAGGGAGCTTTTGGTTATGGTTCCTATTTTTCACTATCAATGATGGACAGACACTTCCATAGCGGAATGAACGTGGAAGAAGCAATTGATCTTGTTGATAAGTGCATTTTGGAAATCAGATCAAGGTTGGTTGTGGCACCTCCAAACTTTGTTATAAAAATTGTTGACAAGGATGGTGCAAGAGAGTACGCATGGCGCGAATCAGTCAAGGACACTCCTGCTTCAGCTTGA
Protein sequence

>MS.gene024828.t1

MECVFGLVGNGFAIVVADTSAVHSILVHKSNEDKIMFLDSHKLIAASGEPGDRVQFTEYIQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMNVEEAIDLVDKCILEIRSRLVVAPPNFVIKIVDKDGAREYAWRESVKDTPASA