Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29922.t1 | XP_013445564.1 | 99.6 | 246 | 1 | 0 | 1 | 246 | 1 | 246 | 3.00E-135 | 491.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29922.t1 | O48551 | 93.9 | 246 | 15 | 0 | 1 | 246 | 1 | 246 | 1.7e-131 | 469.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29922.t1 | B7FMD3 | 99.6 | 246 | 1 | 0 | 1 | 246 | 1 | 246 | 2.1e-135 | 491.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29922.t1 | MTR_8g464460 | 99.593 | 246 | 1 | 0 | 1 | 246 | 1 | 246 | 0.0 | 509 |
| MS.gene29922.t1 | MTR_2g060830 | 93.089 | 246 | 17 | 0 | 1 | 246 | 1 | 246 | 7.97e-175 | 481 |
| MS.gene29922.t1 | MTR_0167s0060 | 38.462 | 234 | 139 | 4 | 9 | 242 | 6 | 234 | 4.92e-48 | 159 |
| MS.gene29922.t1 | MTR_7g070070 | 42.211 | 199 | 111 | 3 | 9 | 206 | 5 | 200 | 1.56e-44 | 150 |
| MS.gene29922.t1 | MTR_5g016590 | 41.709 | 199 | 112 | 3 | 9 | 206 | 5 | 200 | 3.19e-43 | 147 |
| MS.gene29922.t1 | MTR_8g075320 | 38.428 | 229 | 133 | 5 | 9 | 236 | 4 | 225 | 2.40e-41 | 142 |
| MS.gene29922.t1 | MTR_1g112250 | 34.199 | 231 | 142 | 5 | 9 | 236 | 8 | 231 | 1.87e-40 | 139 |
| MS.gene29922.t1 | MTR_2g016050 | 37.946 | 224 | 127 | 5 | 1 | 223 | 1 | 213 | 2.66e-39 | 137 |
| MS.gene29922.t1 | MTR_1g022410 | 35.217 | 230 | 143 | 5 | 9 | 237 | 6 | 230 | 1.85e-38 | 135 |
| MS.gene29922.t1 | MTR_6g024135 | 36.932 | 176 | 109 | 2 | 6 | 181 | 5 | 178 | 2.81e-32 | 118 |
| MS.gene29922.t1 | MTR_4g012740 | 28.625 | 269 | 146 | 10 | 9 | 237 | 237 | 499 | 2.50e-21 | 92.8 |
| MS.gene29922.t1 | MTR_4g057090 | 26.484 | 219 | 139 | 7 | 12 | 220 | 91 | 297 | 1.31e-15 | 75.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29922.t1 | AT5G35590 | 85.772 | 246 | 35 | 0 | 1 | 246 | 1 | 246 | 5.25e-166 | 458 |
| MS.gene29922.t1 | AT2G05840 | 85.772 | 246 | 35 | 0 | 1 | 246 | 1 | 246 | 1.18e-164 | 455 |
| MS.gene29922.t1 | AT2G05840 | 87.558 | 217 | 27 | 0 | 1 | 217 | 1 | 217 | 5.74e-148 | 412 |
| MS.gene29922.t1 | AT2G05840 | 84.925 | 199 | 30 | 0 | 1 | 199 | 1 | 199 | 2.16e-132 | 372 |
| MS.gene29922.t1 | AT1G16470 | 37.179 | 234 | 142 | 4 | 9 | 242 | 6 | 234 | 1.73e-45 | 152 |
| MS.gene29922.t1 | AT1G16470 | 37.179 | 234 | 142 | 4 | 9 | 242 | 6 | 234 | 1.73e-45 | 152 |
| MS.gene29922.t1 | AT1G79210 | 37.179 | 234 | 142 | 4 | 9 | 242 | 6 | 234 | 3.44e-45 | 151 |
| MS.gene29922.t1 | AT1G79210 | 37.179 | 234 | 142 | 4 | 9 | 242 | 6 | 234 | 3.44e-45 | 151 |
| MS.gene29922.t1 | AT1G79210 | 37.179 | 234 | 142 | 4 | 9 | 242 | 6 | 234 | 3.44e-45 | 151 |
| MS.gene29922.t1 | AT5G66140 | 36.681 | 229 | 138 | 4 | 9 | 236 | 4 | 226 | 2.21e-42 | 145 |
| MS.gene29922.t1 | AT3G51260 | 36.681 | 229 | 138 | 4 | 9 | 236 | 4 | 226 | 4.31e-42 | 144 |
| MS.gene29922.t1 | AT3G22110 | 40.704 | 199 | 114 | 3 | 9 | 206 | 5 | 200 | 8.29e-42 | 143 |
| MS.gene29922.t1 | AT1G53850 | 35.065 | 231 | 140 | 5 | 9 | 236 | 8 | 231 | 2.06e-41 | 142 |
| MS.gene29922.t1 | AT1G53850 | 35.065 | 231 | 140 | 5 | 9 | 236 | 8 | 231 | 2.06e-41 | 142 |
| MS.gene29922.t1 | AT3G14290 | 34.632 | 231 | 141 | 5 | 9 | 236 | 8 | 231 | 5.40e-41 | 140 |
| MS.gene29922.t1 | AT3G51260 | 37.143 | 210 | 127 | 3 | 9 | 217 | 4 | 209 | 5.31e-40 | 138 |
| MS.gene29922.t1 | AT1G47250 | 36.404 | 228 | 139 | 5 | 9 | 235 | 6 | 228 | 2.45e-38 | 135 |
| MS.gene29922.t1 | AT5G42790 | 35.526 | 228 | 141 | 5 | 9 | 235 | 6 | 228 | 1.86e-37 | 132 |
| MS.gene29922.t1 | AT2G27020 | 36.022 | 186 | 111 | 4 | 6 | 185 | 5 | 188 | 2.88e-31 | 116 |
| MS.gene29922.t1 | AT2G27020 | 36.216 | 185 | 110 | 4 | 6 | 184 | 107 | 289 | 2.66e-30 | 115 |
| MS.gene29922.t1 | AT4G15165 | 32.161 | 199 | 89 | 4 | 9 | 206 | 5 | 158 | 9.43e-25 | 97.8 |
Find 64 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAAAGTACCTTGGTTTAC+TGG | 0.220686 | 8.3:-45849066 | MS.gene29922:CDS |
| GAGTGAAGTTGCTATGGTTC+TGG | 0.269202 | 8.3:-45847307 | MS.gene29922:intron |
| GGCCACAAGTGCTGGATTAA+AGG | 0.275884 | 8.3:-45846183 | MS.gene29922:CDS |
| TTGTGTGGTTACTCAGAAAA+AGG | 0.276202 | 8.3:-45849227 | MS.gene29922:CDS |
| CCAAGAGGCAATCAATTTCT+TGG | 0.284591 | 8.3:-45846159 | MS.gene29922:CDS |
| AAACCAAGGTACTTTGTAAT+AGG | 0.311899 | 8.3:+45849071 | None:intergenic |
| GGATAACCTCGATTGGTGTT+AGG | 0.343827 | 8.3:-45849264 | MS.gene29922:CDS |
| CTGTGGATGTACTGTCTAAA+TGG | 0.352899 | 8.3:-45847967 | MS.gene29922:intron |
| AACTGATTGCAAGGCAGAAA+TGG | 0.358222 | 8.3:+45839263 | None:intergenic |
| CTGGGTATTGACGATGAACT+TGG | 0.365337 | 8.3:-45847288 | MS.gene29922:CDS |
| GTGGACAAAACTCTAAATTC+TGG | 0.373989 | 8.3:+45838966 | None:intergenic |
| ATGAGTAGAGGAAGCGGAGG+TGG | 0.377515 | 8.3:-45850279 | MS.gene29922:CDS |
| TTCAAGGCTGTTAAGTCTGC+AGG | 0.383896 | 8.3:-45849286 | MS.gene29922:CDS |
| CAGGGTCACATTTGTAAAGC+TGG | 0.386432 | 8.3:+45847262 | None:intergenic |
| TACCTATGCCGAGACGGTTC+AGG | 0.388567 | 8.3:-45846108 | MS.gene29922:intron |
| TCAAACAGAGTATGCTTTCA+AGG | 0.394882 | 8.3:-45849302 | MS.gene29922:intron |
| GCTGAGTATCGCTTTAGATA+TGG | 0.406865 | 8.3:-45848001 | MS.gene29922:CDS |
| GATAACCTCGATTGGTGTTA+GGG | 0.418833 | 8.3:-45849263 | MS.gene29922:CDS |
| CCAAGAAATTGATTGCCTCT+TGG | 0.430454 | 8.3:+45846159 | None:intergenic |
| TGTACTCAGGCCACAAGTGC+TGG | 0.430811 | 8.3:-45846191 | MS.gene29922:intron |
| AGTGAAGTTGCTATGGTTCT+GGG | 0.439091 | 8.3:-45847306 | MS.gene29922:intron |
| GTCCTTTAATCCAGCACTTG+TGG | 0.454543 | 8.3:+45846181 | None:intergenic |
| TGCTTGTTGAACTAGTGTCC+TGG | 0.461152 | 8.3:+45848036 | None:intergenic |
| GAAAAGATGAGTAGAGGAAG+CGG | 0.477094 | 8.3:-45850285 | None:intergenic |
| GACCCTGCTGGTCATTACTT+TGG | 0.498366 | 8.3:-45847246 | MS.gene29922:CDS |
| ACTTGAAAGAGGCGCCCCTC+CGG | 0.501215 | 8.3:+45850228 | None:intergenic |
| AGGGTCACATTTGTAAAGCT+GGG | 0.502939 | 8.3:+45847263 | None:intergenic |
| GTCTCGGCATAGGTAAATGA+AGG | 0.506661 | 8.3:+45846116 | None:intergenic |
| TACCTTGGTTTACTGGCCAC+TGG | 0.507261 | 8.3:-45849059 | MS.gene29922:CDS |
| AGAGATGTGTAACACTTGTA+TGG | 0.512075 | 8.3:+45849096 | None:intergenic |
| TTACTGGCCACTGGCATCAC+AGG | 0.515432 | 8.3:-45849050 | MS.gene29922:intron |
| TGACCAAAGTAATGACCAGC+AGG | 0.522657 | 8.3:+45847243 | None:intergenic |
| CTTGCAATCAGTTCTTCAAG+AGG | 0.529591 | 8.3:-45839253 | MS.gene29922:CDS |
| AAGATGAGTAGAGGAAGCGG+AGG | 0.536838 | 8.3:-45850282 | MS.gene29922:CDS |
| CGTACGTACCGACTTGAAAG+AGG | 0.540031 | 8.3:+45850217 | None:intergenic |
| CATGCTTATATGAGACCACT+TGG | 0.542442 | 8.3:-45847489 | MS.gene29922:CDS |
| TTTCCTATTACAAAGTACCT+TGG | 0.543094 | 8.3:-45849074 | MS.gene29922:CDS |
| CTAGGTTGGAGTTGTGCAGA+AGG | 0.553908 | 8.3:-45838994 | MS.gene29922:intron |
| TTCATTTACCTATGCCGAGA+CGG | 0.554212 | 8.3:-45846114 | MS.gene29922:CDS |
| CTTTACAAATGTGACCCTGC+TGG | 0.558651 | 8.3:-45847258 | MS.gene29922:CDS |
| CTCTTGAAGAACTGATTGCA+AGG | 0.559622 | 8.3:+45839254 | None:intergenic |
| GGGAAAAGATTCAGTTTGTG+TGG | 0.559768 | 8.3:-45849242 | MS.gene29922:CDS |
| AAATATTACCTGAACCGTCT+CGG | 0.561908 | 8.3:+45846100 | None:intergenic |
| TGGGTATTGACGATGAACTT+GGG | 0.570691 | 8.3:-45847287 | MS.gene29922:CDS |
| ATTGTGGAGTGAAGTTGCTA+TGG | 0.574803 | 8.3:-45847313 | MS.gene29922:intron |
| TACCTGAACCGTCTCGGCAT+AGG | 0.580613 | 8.3:+45846106 | None:intergenic |
| TCTGCAGGGATAACCTCGAT+TGG | 0.594530 | 8.3:-45849271 | MS.gene29922:CDS |
| AATTAAGAAAAGATGAGTAG+AGG | 0.602480 | 8.3:-45850291 | None:intergenic |
| TCAAGGCTGTTAAGTCTGCA+GGG | 0.604847 | 8.3:-45849285 | MS.gene29922:CDS |
| TATATGAGACCACTTGGAGT+AGG | 0.609958 | 8.3:-45847483 | MS.gene29922:intron |
| CATTTAGACAGTACATCCAC+AGG | 0.610314 | 8.3:+45847968 | None:intergenic |
| GTGCTCGTCAATTTCGTCGG+TGG | 0.614681 | 8.3:+45838947 | None:intergenic |
| CAAGTGCTCGTCAATTTCGT+CGG | 0.620286 | 8.3:+45838944 | None:intergenic |
| TGGTCATTACTTTGGTCACA+AGG | 0.621200 | 8.3:-45847238 | MS.gene29922:intron |
| TGCTGGATTAAAGGACCAAG+AGG | 0.623889 | 8.3:-45846174 | MS.gene29922:CDS |
| GAGGGGCGCCTCTTTCAAGT+CGG | 0.649322 | 8.3:-45850225 | MS.gene29922:intron |
| AAACACATACCTACTCCAAG+TGG | 0.652321 | 8.3:+45847474 | None:intergenic |
| GGGTCACATTTGTAAAGCTG+GGG | 0.657277 | 8.3:+45847264 | None:intergenic |
| TGCCAGTGGCCAGTAAACCA+AGG | 0.682988 | 8.3:+45849057 | None:intergenic |
| TCTCGGCATAGGTAAATGAA+GGG | 0.686969 | 8.3:+45846117 | None:intergenic |
| ATAACCTCGATTGGTGTTAG+GGG | 0.701420 | 8.3:-45849262 | MS.gene29922:CDS |
| ATATGGATATGAGATGCCTG+TGG | 0.717907 | 8.3:-45847984 | MS.gene29922:CDS |
| GACCAAAGTAATGACCAGCA+GGG | 0.719039 | 8.3:+45847244 | None:intergenic |
| GAACATACCTGTGATGCCAG+TGG | 0.749474 | 8.3:+45849043 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 45838920 | 45850301 | 45838920 | ID=MS.gene29922 |
| chr8.3 | mRNA | 45838920 | 45850301 | 45838920 | ID=MS.gene29922.t1;Parent=MS.gene29922 |
| chr8.3 | exon | 45850226 | 45850301 | 45850226 | ID=MS.gene29922.t1.exon1;Parent=MS.gene29922.t1 |
| chr8.3 | CDS | 45850226 | 45850301 | 45850226 | ID=cds.MS.gene29922.t1;Parent=MS.gene29922.t1 |
| chr8.3 | exon | 45849222 | 45849316 | 45849222 | ID=MS.gene29922.t1.exon2;Parent=MS.gene29922.t1 |
| chr8.3 | CDS | 45849222 | 45849316 | 45849222 | ID=cds.MS.gene29922.t1;Parent=MS.gene29922.t1 |
| chr8.3 | exon | 45849051 | 45849132 | 45849051 | ID=MS.gene29922.t1.exon3;Parent=MS.gene29922.t1 |
| chr8.3 | CDS | 45849051 | 45849132 | 45849051 | ID=cds.MS.gene29922.t1;Parent=MS.gene29922.t1 |
| chr8.3 | exon | 45847968 | 45848064 | 45847968 | ID=MS.gene29922.t1.exon4;Parent=MS.gene29922.t1 |
| chr8.3 | CDS | 45847968 | 45848064 | 45847968 | ID=cds.MS.gene29922.t1;Parent=MS.gene29922.t1 |
| chr8.3 | exon | 45847484 | 45847542 | 45847484 | ID=MS.gene29922.t1.exon5;Parent=MS.gene29922.t1 |
| chr8.3 | CDS | 45847484 | 45847542 | 45847484 | ID=cds.MS.gene29922.t1;Parent=MS.gene29922.t1 |
| chr8.3 | exon | 45847239 | 45847321 | 45847239 | ID=MS.gene29922.t1.exon6;Parent=MS.gene29922.t1 |
| chr8.3 | CDS | 45847239 | 45847321 | 45847239 | ID=cds.MS.gene29922.t1;Parent=MS.gene29922.t1 |
| chr8.3 | exon | 45846109 | 45846204 | 45846109 | ID=MS.gene29922.t1.exon7;Parent=MS.gene29922.t1 |
| chr8.3 | CDS | 45846109 | 45846204 | 45846109 | ID=cds.MS.gene29922.t1;Parent=MS.gene29922.t1 |
| chr8.3 | exon | 45839230 | 45839289 | 45839230 | ID=MS.gene29922.t1.exon8;Parent=MS.gene29922.t1 |
| chr8.3 | CDS | 45839230 | 45839289 | 45839230 | ID=cds.MS.gene29922.t1;Parent=MS.gene29922.t1 |
| chr8.3 | exon | 45838920 | 45839012 | 45838920 | ID=MS.gene29922.t1.exon9;Parent=MS.gene29922.t1 |
| chr8.3 | CDS | 45838920 | 45839012 | 45838920 | ID=cds.MS.gene29922.t1;Parent=MS.gene29922.t1 |
| Gene Sequence |
| Protein sequence |
