Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025808.t1 | XP_003611299.1 | 99 | 204 | 2 | 0 | 1 | 204 | 1 | 204 | 6.10E-110 | 406.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025808.t1 | O23714 | 89.4 | 198 | 21 | 0 | 1 | 198 | 1 | 198 | 7.1e-99 | 361.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025808.t1 | B7FH05 | 99.0 | 204 | 2 | 0 | 1 | 204 | 1 | 204 | 4.4e-110 | 406.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025808.t1 | MTR_5g012470 | 99.020 | 204 | 2 | 0 | 1 | 204 | 1 | 204 | 1.25e-152 | 421 |
| MS.gene025808.t1 | MTR_5g006840 | 96.000 | 200 | 8 | 0 | 1 | 200 | 1 | 200 | 5.25e-146 | 404 |
| MS.gene025808.t1 | MTR_2g050000 | 91.429 | 105 | 7 | 1 | 95 | 199 | 2 | 104 | 3.43e-67 | 201 |
| MS.gene025808.t1 | MTR_1g112250 | 28.249 | 177 | 114 | 4 | 10 | 178 | 42 | 213 | 9.47e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025808.t1 | AT3G22630 | 89.394 | 198 | 21 | 0 | 1 | 198 | 1 | 198 | 3.42e-134 | 374 |
| MS.gene025808.t1 | AT4G14800 | 87.374 | 198 | 25 | 0 | 1 | 198 | 1 | 198 | 1.62e-130 | 365 |
| MS.gene025808.t1 | AT4G14800 | 80.841 | 214 | 25 | 1 | 1 | 198 | 1 | 214 | 5.35e-126 | 354 |
Find 42 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAACCATGGAATGCGTATT+TGG | 0.216378 | 5.3:+5614617 | None:intergenic |
| CTTTGTATCAATTCCGTAAT+GGG | 0.276520 | 5.3:+5614886 | MS.gene025808:CDS |
| AAGATTGAAAAGGGTGCTTT+TGG | 0.320193 | 5.3:+5616151 | MS.gene025808:CDS |
| TCTGTGAACATCCTTCTTGC+TGG | 0.377484 | 5.3:+5616070 | MS.gene025808:CDS |
| AGAAGGATGTTCACAGAGTA+GGG | 0.377993 | 5.3:-5616064 | None:intergenic |
| GCTTTGTATCAATTCCGTAA+TGG | 0.426748 | 5.3:+5614885 | MS.gene025808:CDS |
| AACACTTCACAAGATTGAAA+AGG | 0.450418 | 5.3:+5616141 | MS.gene025808:CDS |
| CTTCCACAGACATTCCACTA+TGG | 0.474885 | 5.3:-5616216 | None:intergenic |
| AAGAAGGATGTTCACAGAGT+AGG | 0.478344 | 5.3:-5616065 | None:intergenic |
| TTAATTCAAGCTGAAGGAAC+AGG | 0.478420 | 5.3:-5616376 | None:intergenic |
| CTAGCGGAGAACCCGGTGAC+AGG | 0.480056 | 5.3:+5614759 | MS.gene025808:CDS |
| GAATGCGTATTTGGATTAGT+AGG | 0.482356 | 5.3:+5614626 | MS.gene025808:CDS |
| TAGTGAGAGGGATCCCATTA+CGG | 0.491022 | 5.3:-5614899 | None:intergenic |
| AATCAGATCAAGGTTGGTTG+TGG | 0.521276 | 5.3:+5616273 | MS.gene025808:CDS |
| ACACTTCACAAGATTGAAAA+GGG | 0.531864 | 5.3:+5616142 | MS.gene025808:CDS |
| TGCGGAAATCAGATCAAGGT+TGG | 0.532272 | 5.3:+5616267 | MS.gene025808:CDS |
| TAGTCAATATAGTATAGTGA+TGG | 0.533828 | 5.3:-5616112 | None:intergenic |
| ATCAAAATTGTTGACAAGGA+TGG | 0.541090 | 5.3:+5616313 | MS.gene025808:CDS |
| ACTCGCTACTGCGCTCCGCA+AGG | 0.542105 | 5.3:+5614953 | MS.gene025808:CDS |
| GCTGGTTATGACAAAGAGAC+AGG | 0.546617 | 5.3:+5616088 | MS.gene025808:CDS |
| TAATCCAAATACGCATTCCA+TGG | 0.558419 | 5.3:-5614621 | None:intergenic |
| CACAAACTCGTTGCTGCTAG+CGG | 0.562076 | 5.3:+5614743 | MS.gene025808:CDS |
| GAGAGAATGAAACCTGTCAC+CGG | 0.562718 | 5.3:-5614771 | None:intergenic |
| TGTTATCAAAATTGTTGACA+AGG | 0.566229 | 5.3:+5616309 | MS.gene025808:CDS |
| CTTTGTCATAACCAGCAAGA+AGG | 0.572175 | 5.3:-5616081 | None:intergenic |
| CAGCAGCAGCAGTAGTGAGA+GGG | 0.586152 | 5.3:-5614911 | None:intergenic |
| GCAGCAGCAGCAGTAGTGAG+AGG | 0.587393 | 5.3:-5614912 | None:intergenic |
| ATGGACAGACACTACCATAG+TGG | 0.594448 | 5.3:+5616202 | MS.gene025808:CDS |
| AGTCAATATAGTATAGTGAT+GGG | 0.602500 | 5.3:-5616111 | None:intergenic |
| GCATTGCGGAAATCAGATCA+AGG | 0.606697 | 5.3:+5616263 | MS.gene025808:CDS |
| ATACGGTTAATTCAAGCTGA+AGG | 0.609793 | 5.3:-5616382 | None:intergenic |
| GTTGCTGCTAGCGGAGAACC+CGG | 0.616843 | 5.3:+5614752 | MS.gene025808:CDS |
| CTACCATAGTGGAATGTCTG+TGG | 0.623177 | 5.3:+5616213 | MS.gene025808:CDS |
| TGCATGGCGTCAATCAGTGA+AGG | 0.631931 | 5.3:+5616348 | MS.gene025808:CDS |
| ATGGTGCAAGAGAGTATGCA+TGG | 0.636054 | 5.3:+5616332 | MS.gene025808:CDS |
| CTTCATTTGATTTGTGTACG+AGG | 0.644073 | 5.3:-5614697 | None:intergenic |
| AGAGAATGAAACCTGTCACC+GGG | 0.644260 | 5.3:-5614770 | None:intergenic |
| GCTGCTGCTAATTTCACTCG+TGG | 0.646433 | 5.3:+5614927 | MS.gene025808:CDS |
| AGATGTATCAGCTACTACGA+TGG | 0.672169 | 5.3:-5614660 | None:intergenic |
| CAACGAGTTTGTGAGAATCG+AGG | 0.687571 | 5.3:-5614733 | None:intergenic |
| GAAGGATGTTCACAGAGTAG+GGG | 0.691431 | 5.3:-5616063 | None:intergenic |
| TCTTGTTGATAAGTGCATTG+CGG | 0.710102 | 5.3:+5616249 | MS.gene025808:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGCTAAAGTTTAATATT+TGG | + | chr5.3:5615070-5615089 | MS.gene025808:intron | 15.0% |
| !!! | ACTTATTTAAGATTATCTAT+TGG | + | chr5.3:5615120-5615139 | MS.gene025808:intron | 15.0% |
| !! | AAAGTATAGCACTAATTATT+AGG | - | chr5.3:5615004-5615023 | None:intergenic | 20.0% |
| !! | AAGTATAGCACTAATTATTA+GGG | - | chr5.3:5615003-5615022 | None:intergenic | 20.0% |
| !! | AATAAGTTGTTTATCTAAAC+AGG | + | chr5.3:5615853-5615872 | MS.gene025808:intron | 20.0% |
| !! | AATTCAAAATCAAAACTTGA+AGG | - | chr5.3:5614807-5614826 | None:intergenic | 20.0% |
| !! | TAATACTTGTAAGACTATTT+GGG | + | chr5.3:5615146-5615165 | MS.gene025808:intron | 20.0% |
| !! | TTAATACTTGTAAGACTATT+TGG | + | chr5.3:5615145-5615164 | MS.gene025808:intron | 20.0% |
| !!! | ACAATTTTGATAACAAAGTT+TGG | - | chr5.3:5616304-5616323 | None:intergenic | 20.0% |
| !!! | ATTCATTGTTTTTCACTTTT+AGG | + | chr5.3:5614829-5614848 | MS.gene025808:intron | 20.0% |
| !!! | TAATATTTGGATTTAGTTCA+TGG | + | chr5.3:5615083-5615102 | MS.gene025808:intron | 20.0% |
| !!! | TTCATTGTTTTTCACTTTTA+GGG | + | chr5.3:5614830-5614849 | MS.gene025808:intron | 20.0% |
| ! | AATTATTAGGGTTTGGAAAA+AGG | - | chr5.3:5614991-5615010 | None:intergenic | 25.0% |
| ! | AGTCAATATAGTATAGTGAT+GGG | - | chr5.3:5616114-5616133 | None:intergenic | 25.0% |
| ! | ATTATTAGGGTTTGGAAAAA+GGG | - | chr5.3:5614990-5615009 | None:intergenic | 25.0% |
| ! | CTTATCATGATAAGCATTTA+TGG | - | chr5.3:5615813-5615832 | None:intergenic | 25.0% |
| ! | TAGTCAATATAGTATAGTGA+TGG | - | chr5.3:5616115-5616134 | None:intergenic | 25.0% |
| ! | TGTTATCAAAATTGTTGACA+AGG | + | chr5.3:5616309-5616328 | MS.gene025808:CDS | 25.0% |
| !!! | ATTTTGATAACAAAGTTTGG+AGG | - | chr5.3:5616301-5616320 | None:intergenic | 25.0% |
| !!! | CATCATTGATAGTGAAAAAT+AGG | - | chr5.3:5616185-5616204 | None:intergenic | 25.0% |
| !!! | CTATTTTTCACTATCAATGA+TGG | + | chr5.3:5616183-5616202 | MS.gene025808:CDS | 25.0% |
| AAACAGAGCTAATGTCAATT+TGG | + | chr5.3:5615905-5615924 | MS.gene025808:intron | 30.0% | |
| AACACTTCACAAGATTGAAA+AGG | + | chr5.3:5616141-5616160 | MS.gene025808:CDS | 30.0% | |
| ACACTTCACAAGATTGAAAA+GGG | + | chr5.3:5616142-5616161 | MS.gene025808:CDS | 30.0% | |
| AGTGTATTTATACGTGAACT+TGG | + | chr5.3:5616027-5616046 | MS.gene025808:intron | 30.0% | |
| ATCAAAATTGTTGACAAGGA+TGG | + | chr5.3:5616313-5616332 | MS.gene025808:CDS | 30.0% | |
| ATGTTGTCAAATAGCAGAAT+TGG | - | chr5.3:5615442-5615461 | None:intergenic | 30.0% | |
| CTTTGTATCAATTCCGTAAT+GGG | + | chr5.3:5614886-5614905 | MS.gene025808:CDS | 30.0% | |
| TAGCACTAATTATTAGGGTT+TGG | - | chr5.3:5614998-5615017 | None:intergenic | 30.0% | |
| TGTTGTCAAATAGCAGAATT+GGG | - | chr5.3:5615441-5615460 | None:intergenic | 30.0% | |
| TTATTAGGGTTTGGAAAAAG+GGG | - | chr5.3:5614989-5615008 | None:intergenic | 30.0% | |
| TTGCATTGTGATTATCGATA+AGG | + | chr5.3:5616004-5616023 | MS.gene025808:intron | 30.0% | |
| ! | ATTTAGTTCATGGACTTGTT+TGG | + | chr5.3:5615093-5615112 | MS.gene025808:intron | 30.0% |
| !! | AAATTGACATTAGCTCTGTT+TGG | - | chr5.3:5615906-5615925 | None:intergenic | 30.0% |
| !! | TTATGCTTTTGTTTGGACTT+TGG | + | chr5.3:5615579-5615598 | MS.gene025808:intron | 30.0% |
| AACAGGTTAACTGAACACTT+AGG | - | chr5.3:5615880-5615899 | None:intergenic | 35.0% | |
| ACAGGTTAACTGAACACTTA+GGG | - | chr5.3:5615879-5615898 | None:intergenic | 35.0% | |
| ATCGTCTAATCTCAATCCAA+CGG | + | chr5.3:5615363-5615382 | MS.gene025808:intron | 35.0% | |
| ATTCAGCTACTGATATGCTT+AGG | + | chr5.3:5615951-5615970 | MS.gene025808:intron | 35.0% | |
| GCTTTGTATCAATTCCGTAA+TGG | + | chr5.3:5614885-5614904 | MS.gene025808:CDS | 35.0% | |
| TCGATAATCACAATGCAACA+TGG | - | chr5.3:5616002-5616021 | None:intergenic | 35.0% | |
| TTACTCCTAAGTGCTTTCAT+GGG | + | chr5.3:5615041-5615060 | MS.gene025808:intron | 35.0% | |
| TTTACTCCTAAGTGCTTTCA+TGG | + | chr5.3:5615040-5615059 | MS.gene025808:intron | 35.0% | |
| ! | TAAGCGCTTATGCTTTTGTT+TGG | + | chr5.3:5615572-5615591 | MS.gene025808:intron | 35.0% |
| ! | TCTTGTTGATAAGTGCATTG+CGG | + | chr5.3:5616249-5616268 | MS.gene025808:CDS | 35.0% |
| !! | CTTCATTTGATTTGTGTACG+AGG | - | chr5.3:5614700-5614719 | None:intergenic | 35.0% |
| !! | CTTTTGTTTGGACTTTGGAT+TGG | + | chr5.3:5615584-5615603 | MS.gene025808:intron | 35.0% |
| !! | GAATGCGTATTTGGATTAGT+AGG | + | chr5.3:5614626-5614645 | MS.gene025808:CDS | 35.0% |
| !! | TTAGCTCTGTTTGGATAAAC+AGG | - | chr5.3:5615897-5615916 | None:intergenic | 35.0% |
| !!! | AAGATTGAAAAGGGTGCTTT+TGG | + | chr5.3:5616151-5616170 | MS.gene025808:CDS | 35.0% |
| AAGAAGGATGTTCACAGAGT+AGG | - | chr5.3:5616068-5616087 | None:intergenic | 40.0% | |
| ACTCGGGCTTATCTGTTATT+TGG | + | chr5.3:5615610-5615629 | MS.gene025808:intron | 40.0% | |
| AGAAGGATGTTCACAGAGTA+GGG | - | chr5.3:5616067-5616086 | None:intergenic | 40.0% | |
| AGATGTATCAGCTACTACGA+TGG | - | chr5.3:5614663-5614682 | None:intergenic | 40.0% | |
| AGTGTATCGCAGAACAATAG+TGG | + | chr5.3:5615474-5615493 | MS.gene025808:intron | 40.0% | |
| ATAACAGTATAGCACTGCTG+TGG | + | chr5.3:5615519-5615538 | MS.gene025808:intron | 40.0% | |
| ATGGCTAAATGCGTGAATGT+GGG | + | chr5.3:5615394-5615413 | MS.gene025808:intron | 40.0% | |
| CAATCCAACGGTCACTTATA+TGG | + | chr5.3:5615375-5615394 | MS.gene025808:intron | 40.0% | |
| CTTTGTCATAACCAGCAAGA+AGG | - | chr5.3:5616084-5616103 | None:intergenic | 40.0% | |
| GCTATACTGTTATAGCGTAG+CGG | - | chr5.3:5615512-5615531 | None:intergenic | 40.0% | |
| GGTTTGGAAAAAGGGGTAAA+TGG | - | chr5.3:5614982-5615001 | None:intergenic | 40.0% | |
| GTGTCAGTATAGTCGCAATT+TGG | + | chr5.3:5615213-5615232 | MS.gene025808:intron | 40.0% | |
| TATGGCTAAATGCGTGAATG+TGG | + | chr5.3:5615393-5615412 | MS.gene025808:intron | 40.0% | |
| TTAGCCATATAAGTGACCGT+TGG | - | chr5.3:5615382-5615401 | None:intergenic | 40.0% | |
| TTGGACTGTGTGATACTGTT+TGG | + | chr5.3:5615629-5615648 | MS.gene025808:intron | 40.0% | |
| ! | AATCAGATCAAGGTTGGTTG+TGG | + | chr5.3:5616273-5616292 | MS.gene025808:CDS | 40.0% |
| !! | ATCAACCCATGAAAGCACTT+AGG | - | chr5.3:5615049-5615068 | None:intergenic | 40.0% |
| !! | TGAATGTGGGAACTTGTTGA+GGG | + | chr5.3:5615407-5615426 | MS.gene025808:intron | 40.0% |
| !! | TGTTATAGCGTAGCGGATAA+TGG | - | chr5.3:5615505-5615524 | None:intergenic | 40.0% |
| !!! | GAAAAGGGTGCTTTTGGTTA+TGG | + | chr5.3:5616157-5616176 | MS.gene025808:CDS | 40.0% |
| AGAGAATGAAACCTGTCACC+GGG | - | chr5.3:5614773-5614792 | None:intergenic | 45.0% | |
| AGGGGTAAATGGATACCTTG+CGG | - | chr5.3:5614971-5614990 | None:intergenic | 45.0% | |
| ATCACCAAGTTCCCACATTC+AGG | + | chr5.3:5615253-5615272 | MS.gene025808:intron | 45.0% | |
| ATGGACAGACACTACCATAG+TGG | + | chr5.3:5616202-5616221 | MS.gene025808:CDS | 45.0% | |
| CAACGAGTTTGTGAGAATCG+AGG | - | chr5.3:5614736-5614755 | None:intergenic | 45.0% | |
| CTAACAGTCACCCTGAATGT+GGG | - | chr5.3:5615267-5615286 | None:intergenic | 45.0% | |
| CTTCCACAGACATTCCACTA+TGG | - | chr5.3:5616219-5616238 | None:intergenic | 45.0% | |
| GAAGGATGTTCACAGAGTAG+GGG | - | chr5.3:5616066-5616085 | None:intergenic | 45.0% | |
| GAGAGAATGAAACCTGTCAC+CGG | - | chr5.3:5614774-5614793 | None:intergenic | 45.0% | |
| GCATTGCGGAAATCAGATCA+AGG | + | chr5.3:5616263-5616282 | MS.gene025808:CDS | 45.0% | |
| GCTGGTTATGACAAAGAGAC+AGG | + | chr5.3:5616088-5616107 | MS.gene025808:CDS | 45.0% | |
| TAGTGAGAGGGATCCCATTA+CGG | - | chr5.3:5614902-5614921 | None:intergenic | 45.0% | |
| TCACCAAGTTCCCACATTCA+GGG | + | chr5.3:5615254-5615273 | MS.gene025808:intron | 45.0% | |
| TCTAACAGTCACCCTGAATG+TGG | - | chr5.3:5615268-5615287 | None:intergenic | 45.0% | |
| TCTGTGAACATCCTTCTTGC+TGG | + | chr5.3:5616070-5616089 | MS.gene025808:CDS | 45.0% | |
| TGCGGAAATCAGATCAAGGT+TGG | + | chr5.3:5616267-5616286 | MS.gene025808:CDS | 45.0% | |
| ! | ATGGTGCAAGAGAGTATGCA+TGG | + | chr5.3:5616332-5616351 | MS.gene025808:CDS | 45.0% |
| ! | CTACCATAGTGGAATGTCTG+TGG | + | chr5.3:5616213-5616232 | MS.gene025808:CDS | 45.0% |
| !! | GACTTTGGATTGGCTTACTC+GGG | + | chr5.3:5615594-5615613 | MS.gene025808:intron | 45.0% |
| !! | GGACTTTGGATTGGCTTACT+CGG | + | chr5.3:5615593-5615612 | MS.gene025808:intron | 45.0% |
| !! | GTGAATGTGGGAACTTGTTG+AGG | + | chr5.3:5615406-5615425 | MS.gene025808:intron | 45.0% |
| CACAAACTCGTTGCTGCTAG+CGG | + | chr5.3:5614743-5614762 | MS.gene025808:CDS | 50.0% | |
| GCTGCTGCTAATTTCACTCG+TGG | + | chr5.3:5614927-5614946 | MS.gene025808:CDS | 50.0% | |
| TCACCCTGAATGTGGGAACT+TGG | - | chr5.3:5615260-5615279 | None:intergenic | 50.0% | |
| TGCATGGCGTCAATCAGTGA+AGG | + | chr5.3:5616348-5616367 | MS.gene025808:CDS | 50.0% | |
| CAGCAGCAGCAGTAGTGAGA+GGG | - | chr5.3:5614914-5614933 | None:intergenic | 55.0% | |
| GCAGCAGCAGCAGTAGTGAG+AGG | - | chr5.3:5614915-5614934 | None:intergenic | 60.0% | |
| GTTGCTGCTAGCGGAGAACC+CGG | + | chr5.3:5614752-5614771 | MS.gene025808:CDS | 60.0% | |
| ACTCGCTACTGCGCTCCGCA+AGG | + | chr5.3:5614953-5614972 | MS.gene025808:CDS | 65.0% | |
| CTAGCGGAGAACCCGGTGAC+AGG | + | chr5.3:5614759-5614778 | MS.gene025808:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 5614623 | 5616393 | 5614623 | ID=MS.gene025808 |
| chr5.3 | mRNA | 5614623 | 5616393 | 5614623 | ID=MS.gene025808.t1;Parent=MS.gene025808 |
| chr5.3 | exon | 5614623 | 5614780 | 5614623 | ID=MS.gene025808.t1.exon1;Parent=MS.gene025808.t1 |
| chr5.3 | CDS | 5614623 | 5614780 | 5614623 | ID=cds.MS.gene025808.t1;Parent=MS.gene025808.t1 |
| chr5.3 | exon | 5614851 | 5614974 | 5614851 | ID=MS.gene025808.t1.exon2;Parent=MS.gene025808.t1 |
| chr5.3 | CDS | 5614851 | 5614974 | 5614851 | ID=cds.MS.gene025808.t1;Parent=MS.gene025808.t1 |
| chr5.3 | exon | 5616061 | 5616393 | 5616061 | ID=MS.gene025808.t1.exon3;Parent=MS.gene025808.t1 |
| chr5.3 | CDS | 5616061 | 5616393 | 5616061 | ID=cds.MS.gene025808.t1;Parent=MS.gene025808.t1 |
| Gene Sequence |
| Protein sequence |
