Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09974.t1 | XP_003611299.1 | 99 | 204 | 2 | 0 | 1 | 204 | 1 | 204 | 6.10E-110 | 406.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09974.t1 | O23714 | 89.4 | 198 | 21 | 0 | 1 | 198 | 1 | 198 | 7.1e-99 | 361.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09974.t1 | B7FH05 | 99.0 | 204 | 2 | 0 | 1 | 204 | 1 | 204 | 4.4e-110 | 406.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09974.t1 | MTR_5g012470 | 99.020 | 204 | 2 | 0 | 1 | 204 | 1 | 204 | 1.25e-152 | 421 |
| MS.gene09974.t1 | MTR_5g006840 | 96.000 | 200 | 8 | 0 | 1 | 200 | 1 | 200 | 5.25e-146 | 404 |
| MS.gene09974.t1 | MTR_2g050000 | 91.429 | 105 | 7 | 1 | 95 | 199 | 2 | 104 | 3.43e-67 | 201 |
| MS.gene09974.t1 | MTR_1g112250 | 28.249 | 177 | 114 | 4 | 10 | 178 | 42 | 213 | 9.47e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09974.t1 | AT3G22630 | 89.394 | 198 | 21 | 0 | 1 | 198 | 1 | 198 | 3.42e-134 | 374 |
| MS.gene09974.t1 | AT4G14800 | 87.374 | 198 | 25 | 0 | 1 | 198 | 1 | 198 | 1.62e-130 | 365 |
| MS.gene09974.t1 | AT4G14800 | 80.841 | 214 | 25 | 1 | 1 | 198 | 1 | 214 | 5.35e-126 | 354 |
Find 42 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAACCATGGAATGCGTATT+TGG | 0.216378 | 5.2:+4864684 | None:intergenic |
| CTTTGTATCAATTCCGTAAT+GGG | 0.276520 | 5.2:+4864959 | MS.gene09974:CDS |
| AAGATTGAAAAGGGTGCTTT+TGG | 0.320193 | 5.2:+4866224 | MS.gene09974:CDS |
| TCTGTGAACATCCTTCTTGC+TGG | 0.377484 | 5.2:+4866143 | MS.gene09974:CDS |
| AGAAGGATGTTCACAGAGTA+GGG | 0.377993 | 5.2:-4866137 | None:intergenic |
| GCTTTGTATCAATTCCGTAA+TGG | 0.426748 | 5.2:+4864958 | MS.gene09974:CDS |
| AACACTTCACAAGATTGAAA+AGG | 0.450418 | 5.2:+4866214 | MS.gene09974:CDS |
| CTAGCGGTGAACCCGGTGAC+AGG | 0.462278 | 5.2:+4864826 | MS.gene09974:CDS |
| CTTCCACAGACATTCCACTA+TGG | 0.474885 | 5.2:-4866289 | None:intergenic |
| AAGAAGGATGTTCACAGAGT+AGG | 0.478344 | 5.2:-4866138 | None:intergenic |
| TTAATTCAAGCTGAAGGAAC+AGG | 0.478420 | 5.2:-4866449 | None:intergenic |
| GAATGCGTATTTGGATTAGT+AGG | 0.482356 | 5.2:+4864693 | MS.gene09974:CDS |
| TAGTGAGAGGGATCCCATTA+CGG | 0.491022 | 5.2:-4864972 | None:intergenic |
| AATCAGATCAAGGTTGGTTG+TGG | 0.521276 | 5.2:+4866346 | MS.gene09974:CDS |
| ACACTTCACAAGATTGAAAA+GGG | 0.531864 | 5.2:+4866215 | MS.gene09974:CDS |
| TGCGGAAATCAGATCAAGGT+TGG | 0.532272 | 5.2:+4866340 | MS.gene09974:CDS |
| TAGTCAATATAGTATAGTGA+TGG | 0.533828 | 5.2:-4866185 | None:intergenic |
| ATCAAAATTGTTGACAAGGA+TGG | 0.541090 | 5.2:+4866386 | MS.gene09974:CDS |
| ACTCGCTACTGCGCTCCGCA+AGG | 0.542105 | 5.2:+4865026 | MS.gene09974:CDS |
| GCTGGTTATGACAAAGAGAC+AGG | 0.546617 | 5.2:+4866161 | MS.gene09974:CDS |
| TAATCCAAATACGCATTCCA+TGG | 0.558419 | 5.2:-4864688 | None:intergenic |
| GAGAGAATGAAACCTGTCAC+CGG | 0.562718 | 5.2:-4864838 | None:intergenic |
| TGTTATCAAAATTGTTGACA+AGG | 0.566229 | 5.2:+4866382 | MS.gene09974:CDS |
| CACAAACTCGTTGCTGCTAG+CGG | 0.569004 | 5.2:+4864810 | MS.gene09974:CDS |
| CTTTGTCATAACCAGCAAGA+AGG | 0.572175 | 5.2:-4866154 | None:intergenic |
| CAGCAGCAGCAGTAGTGAGA+GGG | 0.586152 | 5.2:-4864984 | None:intergenic |
| GCAGCAGCAGCAGTAGTGAG+AGG | 0.587393 | 5.2:-4864985 | None:intergenic |
| ATGGACAGACACTACCATAG+TGG | 0.594448 | 5.2:+4866275 | MS.gene09974:CDS |
| AGTCAATATAGTATAGTGAT+GGG | 0.602500 | 5.2:-4866184 | None:intergenic |
| GCATTGCGGAAATCAGATCA+AGG | 0.606697 | 5.2:+4866336 | MS.gene09974:CDS |
| ATACGGTTAATTCAAGCTGA+AGG | 0.609793 | 5.2:-4866455 | None:intergenic |
| GTTGCTGCTAGCGGTGAACC+CGG | 0.614126 | 5.2:+4864819 | MS.gene09974:CDS |
| CTACCATAGTGGAATGTCTG+TGG | 0.623177 | 5.2:+4866286 | MS.gene09974:CDS |
| TGCATGGCGTCAATCAGTGA+AGG | 0.631931 | 5.2:+4866421 | MS.gene09974:CDS |
| ATGGTGCAAGAGAGTATGCA+TGG | 0.636054 | 5.2:+4866405 | MS.gene09974:CDS |
| CTTCATTTGATTTGTGTACG+AGG | 0.644073 | 5.2:-4864764 | None:intergenic |
| AGAGAATGAAACCTGTCACC+GGG | 0.644260 | 5.2:-4864837 | None:intergenic |
| GCTGCTGCTAATTTCACTCG+TGG | 0.646433 | 5.2:+4865000 | MS.gene09974:CDS |
| AGATGTATCAGCTACTACGA+TGG | 0.672169 | 5.2:-4864727 | None:intergenic |
| CAACGAGTTTGTGAGAATCG+AGG | 0.687571 | 5.2:-4864800 | None:intergenic |
| GAAGGATGTTCACAGAGTAG+GGG | 0.691431 | 5.2:-4866136 | None:intergenic |
| TCTTGTTGATAAGTGCATTG+CGG | 0.710102 | 5.2:+4866322 | MS.gene09974:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGCTAAAGTTTAATATT+TGG | + | chr5.2:4865143-4865162 | MS.gene09974:intron | 15.0% |
| !!! | ACTTATTTAAGATTATCTAT+TGG | + | chr5.2:4865193-4865212 | MS.gene09974:intron | 15.0% |
| !! | AAAGTATAGCACTAATTATT+AGG | - | chr5.2:4865077-4865096 | None:intergenic | 20.0% |
| !! | AAGTATAGCACTAATTATTA+GGG | - | chr5.2:4865076-4865095 | None:intergenic | 20.0% |
| !! | AATAAGTTGTTTATCTAAAC+AGG | + | chr5.2:4865926-4865945 | MS.gene09974:intron | 20.0% |
| !! | TAATACTTGTAAGACTATTT+GGG | + | chr5.2:4865219-4865238 | MS.gene09974:intron | 20.0% |
| !! | TTAATACTTGTAAGACTATT+TGG | + | chr5.2:4865218-4865237 | MS.gene09974:intron | 20.0% |
| !!! | ACAATTTTGATAACAAAGTT+TGG | - | chr5.2:4866377-4866396 | None:intergenic | 20.0% |
| !!! | ATTCATTGATTTTCACTTTT+AGG | + | chr5.2:4864902-4864921 | MS.gene09974:intron | 20.0% |
| !!! | TAATATTTGGATTTAGTTCA+TGG | + | chr5.2:4865156-4865175 | MS.gene09974:intron | 20.0% |
| !!! | TTCATTGATTTTCACTTTTA+GGG | + | chr5.2:4864903-4864922 | MS.gene09974:intron | 20.0% |
| ! | AACTCAAAATCAAAACTTGA+AGG | - | chr5.2:4864874-4864893 | None:intergenic | 25.0% |
| ! | AATTATTAGGGTTTGGAAAA+AGG | - | chr5.2:4865064-4865083 | None:intergenic | 25.0% |
| ! | AGTCAATATAGTATAGTGAT+GGG | - | chr5.2:4866187-4866206 | None:intergenic | 25.0% |
| ! | ATTATTAGGGTTTGGAAAAA+GGG | - | chr5.2:4865063-4865082 | None:intergenic | 25.0% |
| ! | CTTATCATGATAAGCATTTA+TGG | - | chr5.2:4865886-4865905 | None:intergenic | 25.0% |
| ! | TAGTCAATATAGTATAGTGA+TGG | - | chr5.2:4866188-4866207 | None:intergenic | 25.0% |
| ! | TGTTATCAAAATTGTTGACA+AGG | + | chr5.2:4866382-4866401 | MS.gene09974:CDS | 25.0% |
| !!! | ATTTTGATAACAAAGTTTGG+AGG | - | chr5.2:4866374-4866393 | None:intergenic | 25.0% |
| !!! | CATCATTGATAGTGAAAAAT+AGG | - | chr5.2:4866258-4866277 | None:intergenic | 25.0% |
| !!! | CTATTTTTCACTATCAATGA+TGG | + | chr5.2:4866256-4866275 | MS.gene09974:CDS | 25.0% |
| AAACAGAGCTAATGTCAATT+TGG | + | chr5.2:4865978-4865997 | MS.gene09974:intron | 30.0% | |
| AACACTTCACAAGATTGAAA+AGG | + | chr5.2:4866214-4866233 | MS.gene09974:CDS | 30.0% | |
| ACACTTCACAAGATTGAAAA+GGG | + | chr5.2:4866215-4866234 | MS.gene09974:CDS | 30.0% | |
| AGTGTATTTATACGTGAACT+TGG | + | chr5.2:4866100-4866119 | MS.gene09974:intron | 30.0% | |
| ATCAAAATTGTTGACAAGGA+TGG | + | chr5.2:4866386-4866405 | MS.gene09974:CDS | 30.0% | |
| ATGTTGTCAAATAGCAGAAT+TGG | - | chr5.2:4865515-4865534 | None:intergenic | 30.0% | |
| CTTTGTATCAATTCCGTAAT+GGG | + | chr5.2:4864959-4864978 | MS.gene09974:CDS | 30.0% | |
| TAGCACTAATTATTAGGGTT+TGG | - | chr5.2:4865071-4865090 | None:intergenic | 30.0% | |
| TGTTGTCAAATAGCAGAATT+GGG | - | chr5.2:4865514-4865533 | None:intergenic | 30.0% | |
| TTATTAGGGTTTGGAAAAAG+GGG | - | chr5.2:4865062-4865081 | None:intergenic | 30.0% | |
| TTGCATTGTGATTATCGATA+AGG | + | chr5.2:4866077-4866096 | MS.gene09974:intron | 30.0% | |
| ! | ATTTAGTTCATGGACTTGTT+TGG | + | chr5.2:4865166-4865185 | MS.gene09974:intron | 30.0% |
| !! | AAATTGACATTAGCTCTGTT+TGG | - | chr5.2:4865979-4865998 | None:intergenic | 30.0% |
| !! | TTATGCTTTTGTTTGGACTT+TGG | + | chr5.2:4865652-4865671 | MS.gene09974:intron | 30.0% |
| AACAGGTTAACTGAACACTT+AGG | - | chr5.2:4865953-4865972 | None:intergenic | 35.0% | |
| ACAGGTTAACTGAACACTTA+GGG | - | chr5.2:4865952-4865971 | None:intergenic | 35.0% | |
| ATCGTCTAATCTCAATCCAA+CGG | + | chr5.2:4865436-4865455 | MS.gene09974:intron | 35.0% | |
| ATTCAGCTACTGATATGCTT+AGG | + | chr5.2:4866024-4866043 | MS.gene09974:intron | 35.0% | |
| GCTTTGTATCAATTCCGTAA+TGG | + | chr5.2:4864958-4864977 | MS.gene09974:CDS | 35.0% | |
| TCGATAATCACAATGCAACA+TGG | - | chr5.2:4866075-4866094 | None:intergenic | 35.0% | |
| TTACTCCTAAGTGCTTTCAT+GGG | + | chr5.2:4865114-4865133 | MS.gene09974:intron | 35.0% | |
| TTTACTCCTAAGTGCTTTCA+TGG | + | chr5.2:4865113-4865132 | MS.gene09974:intron | 35.0% | |
| ! | TAAGCGCTTATGCTTTTGTT+TGG | + | chr5.2:4865645-4865664 | MS.gene09974:intron | 35.0% |
| ! | TCTTGTTGATAAGTGCATTG+CGG | + | chr5.2:4866322-4866341 | MS.gene09974:CDS | 35.0% |
| !! | CTTCATTTGATTTGTGTACG+AGG | - | chr5.2:4864767-4864786 | None:intergenic | 35.0% |
| !! | CTTTTGTTTGGACTTTGGAT+TGG | + | chr5.2:4865657-4865676 | MS.gene09974:intron | 35.0% |
| !! | GAATGCGTATTTGGATTAGT+AGG | + | chr5.2:4864693-4864712 | MS.gene09974:CDS | 35.0% |
| !! | TTAGCTCTGTTTGGATAAAC+AGG | - | chr5.2:4865970-4865989 | None:intergenic | 35.0% |
| !!! | AAGATTGAAAAGGGTGCTTT+TGG | + | chr5.2:4866224-4866243 | MS.gene09974:CDS | 35.0% |
| AAGAAGGATGTTCACAGAGT+AGG | - | chr5.2:4866141-4866160 | None:intergenic | 40.0% | |
| ACTCGGGCTTATCTGTTATT+TGG | + | chr5.2:4865683-4865702 | MS.gene09974:intron | 40.0% | |
| AGAAGGATGTTCACAGAGTA+GGG | - | chr5.2:4866140-4866159 | None:intergenic | 40.0% | |
| AGATGTATCAGCTACTACGA+TGG | - | chr5.2:4864730-4864749 | None:intergenic | 40.0% | |
| AGTGTATCGCAGAACAATAG+TGG | + | chr5.2:4865547-4865566 | MS.gene09974:intron | 40.0% | |
| ATAACAGTATAGCACTGCTG+TGG | + | chr5.2:4865592-4865611 | MS.gene09974:intron | 40.0% | |
| ATGGCTAAATGCGTGAATGT+GGG | + | chr5.2:4865467-4865486 | MS.gene09974:intron | 40.0% | |
| CAATCCAACGGTCACTTATA+TGG | + | chr5.2:4865448-4865467 | MS.gene09974:intron | 40.0% | |
| CTTTGTCATAACCAGCAAGA+AGG | - | chr5.2:4866157-4866176 | None:intergenic | 40.0% | |
| GCTATACTGTTATAGCGTAG+CGG | - | chr5.2:4865585-4865604 | None:intergenic | 40.0% | |
| GGTTTGGAAAAAGGGGTAAA+TGG | - | chr5.2:4865055-4865074 | None:intergenic | 40.0% | |
| GTGTCAGTATAGTCGCAATT+TGG | + | chr5.2:4865286-4865305 | MS.gene09974:intron | 40.0% | |
| TATGGCTAAATGCGTGAATG+TGG | + | chr5.2:4865466-4865485 | MS.gene09974:intron | 40.0% | |
| TTAGCCATATAAGTGACCGT+TGG | - | chr5.2:4865455-4865474 | None:intergenic | 40.0% | |
| TTGGACTGTGTGATACTGTT+TGG | + | chr5.2:4865702-4865721 | MS.gene09974:intron | 40.0% | |
| ! | AATCAGATCAAGGTTGGTTG+TGG | + | chr5.2:4866346-4866365 | MS.gene09974:CDS | 40.0% |
| !! | ATCAACCCATGAAAGCACTT+AGG | - | chr5.2:4865122-4865141 | None:intergenic | 40.0% |
| !! | TGAATGTGGGAACTTGTTGA+GGG | + | chr5.2:4865480-4865499 | MS.gene09974:intron | 40.0% |
| !! | TGTTATAGCGTAGCGGATAA+TGG | - | chr5.2:4865578-4865597 | None:intergenic | 40.0% |
| !!! | GAAAAGGGTGCTTTTGGTTA+TGG | + | chr5.2:4866230-4866249 | MS.gene09974:CDS | 40.0% |
| AGAGAATGAAACCTGTCACC+GGG | - | chr5.2:4864840-4864859 | None:intergenic | 45.0% | |
| AGGGGTAAATGGATACCTTG+CGG | - | chr5.2:4865044-4865063 | None:intergenic | 45.0% | |
| ATCACCAAGTTCCCACATTC+AGG | + | chr5.2:4865326-4865345 | MS.gene09974:intron | 45.0% | |
| ATGGACAGACACTACCATAG+TGG | + | chr5.2:4866275-4866294 | MS.gene09974:CDS | 45.0% | |
| CAACGAGTTTGTGAGAATCG+AGG | - | chr5.2:4864803-4864822 | None:intergenic | 45.0% | |
| CTAACAGTCACCCTGAATGT+GGG | - | chr5.2:4865340-4865359 | None:intergenic | 45.0% | |
| CTTCCACAGACATTCCACTA+TGG | - | chr5.2:4866292-4866311 | None:intergenic | 45.0% | |
| GAAGGATGTTCACAGAGTAG+GGG | - | chr5.2:4866139-4866158 | None:intergenic | 45.0% | |
| GAGAGAATGAAACCTGTCAC+CGG | - | chr5.2:4864841-4864860 | None:intergenic | 45.0% | |
| GCATTGCGGAAATCAGATCA+AGG | + | chr5.2:4866336-4866355 | MS.gene09974:CDS | 45.0% | |
| GCTGGTTATGACAAAGAGAC+AGG | + | chr5.2:4866161-4866180 | MS.gene09974:CDS | 45.0% | |
| TAGTGAGAGGGATCCCATTA+CGG | - | chr5.2:4864975-4864994 | None:intergenic | 45.0% | |
| TCACCAAGTTCCCACATTCA+GGG | + | chr5.2:4865327-4865346 | MS.gene09974:intron | 45.0% | |
| TCTAACAGTCACCCTGAATG+TGG | - | chr5.2:4865341-4865360 | None:intergenic | 45.0% | |
| TCTGTGAACATCCTTCTTGC+TGG | + | chr5.2:4866143-4866162 | MS.gene09974:CDS | 45.0% | |
| TGCGGAAATCAGATCAAGGT+TGG | + | chr5.2:4866340-4866359 | MS.gene09974:CDS | 45.0% | |
| ! | ATGGTGCAAGAGAGTATGCA+TGG | + | chr5.2:4866405-4866424 | MS.gene09974:CDS | 45.0% |
| ! | CTACCATAGTGGAATGTCTG+TGG | + | chr5.2:4866286-4866305 | MS.gene09974:CDS | 45.0% |
| !! | GACTTTGGATTGGCTTACTC+GGG | + | chr5.2:4865667-4865686 | MS.gene09974:intron | 45.0% |
| !! | GGACTTTGGATTGGCTTACT+CGG | + | chr5.2:4865666-4865685 | MS.gene09974:intron | 45.0% |
| !! | GTGAATGTGGGAACTTGTTG+AGG | + | chr5.2:4865479-4865498 | MS.gene09974:intron | 45.0% |
| CACAAACTCGTTGCTGCTAG+CGG | + | chr5.2:4864810-4864829 | MS.gene09974:CDS | 50.0% | |
| GCTGCTGCTAATTTCACTCG+TGG | + | chr5.2:4865000-4865019 | MS.gene09974:CDS | 50.0% | |
| TCACCCTGAATGTGGGAACT+TGG | - | chr5.2:4865333-4865352 | None:intergenic | 50.0% | |
| TGCATGGCGTCAATCAGTGA+AGG | + | chr5.2:4866421-4866440 | MS.gene09974:CDS | 50.0% | |
| CAGCAGCAGCAGTAGTGAGA+GGG | - | chr5.2:4864987-4865006 | None:intergenic | 55.0% | |
| GCAGCAGCAGCAGTAGTGAG+AGG | - | chr5.2:4864988-4865007 | None:intergenic | 60.0% | |
| GTTGCTGCTAGCGGTGAACC+CGG | + | chr5.2:4864819-4864838 | MS.gene09974:CDS | 60.0% | |
| ACTCGCTACTGCGCTCCGCA+AGG | + | chr5.2:4865026-4865045 | MS.gene09974:CDS | 65.0% | |
| CTAGCGGTGAACCCGGTGAC+AGG | + | chr5.2:4864826-4864845 | MS.gene09974:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 4864690 | 4866466 | 4864690 | ID=MS.gene09974 |
| chr5.2 | mRNA | 4864690 | 4866466 | 4864690 | ID=MS.gene09974.t1;Parent=MS.gene09974 |
| chr5.2 | exon | 4864690 | 4864847 | 4864690 | ID=MS.gene09974.t1.exon1;Parent=MS.gene09974.t1 |
| chr5.2 | CDS | 4864690 | 4864847 | 4864690 | ID=cds.MS.gene09974.t1;Parent=MS.gene09974.t1 |
| chr5.2 | exon | 4864924 | 4865047 | 4864924 | ID=MS.gene09974.t1.exon2;Parent=MS.gene09974.t1 |
| chr5.2 | CDS | 4864924 | 4865047 | 4864924 | ID=cds.MS.gene09974.t1;Parent=MS.gene09974.t1 |
| chr5.2 | exon | 4866134 | 4866466 | 4866134 | ID=MS.gene09974.t1.exon3;Parent=MS.gene09974.t1 |
| chr5.2 | CDS | 4866134 | 4866466 | 4866134 | ID=cds.MS.gene09974.t1;Parent=MS.gene09974.t1 |
| Gene Sequence |
| Protein sequence |
