AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21801

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21801.t1 MTR_5g006840 100.000 203 0 0 1 203 1 203 5.80e-153 422
MS.gene21801.t1 MTR_5g012470 96.000 200 8 0 1 200 1 200 1.27e-145 403
MS.gene21801.t1 MTR_2g050000 95.413 109 3 1 95 203 2 108 1.31e-71 213
MS.gene21801.t1 MTR_1g112250 30.464 151 94 3 13 155 45 192 8.39e-13 65.5
MS.gene21801.t1 MTR_3g109210 26.087 161 117 1 3 163 15 173 1.72e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21801.t1 AT3G22630 87.129 202 26 0 1 202 1 202 6.55e-135 376
MS.gene21801.t1 AT4G14800 87.374 198 25 0 1 198 1 198 7.00e-132 369
MS.gene21801.t1 AT4G14800 80.841 214 25 1 1 198 1 214 2.21e-127 358
MS.gene21801.t1 AT3G14290 31.126 151 93 3 13 155 45 192 3.05e-13 66.6

Find 42 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGTTGATAAGTGCATTT+TGG 0.220132 5.2:+1494968 MS.gene21801:CDS
CTTTGTATCAATTCCGTAAT+GGG 0.276520 5.2:+1491863 MS.gene21801:CDS
TTTGAATGTATTCAGTAAAC+TGG 0.314079 5.2:-1491831 None:intergenic
AAGCTTGAAAAGGGAGCTTT+TGG 0.328358 5.2:+1494870 MS.gene21801:CDS
TCTGTGAACATCCTTCTTGC+TGG 0.377484 5.2:+1494789 MS.gene21801:CDS
GCTTTGTATCAATTCCGTAA+TGG 0.426748 5.2:+1491862 MS.gene21801:CDS
AGAAGGATGTTCACAGAGTA+GGG 0.434546 5.2:-1494783 None:intergenic
GACACTTCACAAGCTTGAAA+AGG 0.445538 5.2:+1494860 MS.gene21801:CDS
CTAGCGGTGAACCCGGTGAC+AGG 0.462278 5.2:+1491719 MS.gene21801:CDS
AAGAAGGATGTTCACAGAGT+AGG 0.469415 5.2:-1494784 None:intergenic
GAACATGATCTTGTCTTCGT+TGG 0.478439 5.2:-1491671 None:intergenic
TGGTGAGAGGAATCCCATTA+CGG 0.502878 5.2:-1491876 None:intergenic
TTTGGAAATCAGATCAAGGT+TGG 0.503786 5.2:+1494986 MS.gene21801:CDS
ACACTTCACAAGCTTGAAAA+GGG 0.519514 5.2:+1494861 MS.gene21801:CDS
AATCAGATCAAGGTTGGTTG+TGG 0.521276 5.2:+1494992 MS.gene21801:CDS
TGGTAATGGCTTCGCGATCG+TGG 0.521317 5.2:+1491606 MS.gene21801:CDS
GCTTGCCACCGCTCTTCGCA+AGG 0.527948 5.2:+1491930 MS.gene21801:CDS
CTTCCACGTTCATTCCGCTA+TGG 0.534157 5.2:-1494935 None:intergenic
GGATCAACTCAAGCTGAAGC+AGG 0.536274 5.2:-1495095 None:intergenic
GCTGGTTATGACAAAGAGAC+AGG 0.546617 5.2:+1494807 MS.gene21801:CDS
TAGTCAATGTAATATAGTGA+TGG 0.549687 5.2:-1494831 None:intergenic
TAATCCGAAAACGCATTCCA+TGG 0.555612 5.2:-1491581 None:intergenic
ATCGCTGCTAGCGGTGAACC+CGG 0.569631 5.2:+1491712 MS.gene21801:CDS
CTTTGTCATAACCAGCAAGA+AGG 0.572175 5.2:-1494800 None:intergenic
GCGGCAGCAGCTGTGGTGAG+AGG 0.575158 5.2:-1491889 None:intergenic
AGTCAATGTAATATAGTGAT+GGG 0.575452 5.2:-1494830 None:intergenic
AAGAGAGTGAAACCTGTCAC+CGG 0.576291 5.2:-1491731 None:intergenic
GAAATTAGCGGCAGCAGCTG+TGG 0.584921 5.2:-1491896 None:intergenic
CTCGCCACGAGTGAAATTAG+CGG 0.612760 5.2:-1491908 None:intergenic
GAATACCTTGCGAAGAGCGG+TGG 0.614639 5.2:-1491935 None:intergenic
TGCATGGCGCGAATCAGTCA+AGG 0.620623 5.2:+1495067 MS.gene21801:CDS
CTTCGTTGGATTTGTGAACG+AGG 0.622483 5.2:-1491657 None:intergenic
CTTCCATAGCGGAATGAACG+TGG 0.622934 5.2:+1494932 MS.gene21801:CDS
CACAAACTCATCGCTGCTAG+CGG 0.629383 5.2:+1491703 MS.gene21801:CDS
ATGGTGCAAGAGAGTATGCA+TGG 0.636054 5.2:+1495051 MS.gene21801:CDS
AGAGAGTGAAACCTGTCACC+GGG 0.641820 5.2:-1491730 None:intergenic
ATTGAATACCTTGCGAAGAG+CGG 0.643228 5.2:-1491938 None:intergenic
GCTGCCGCTAATTTCACTCG+TGG 0.645223 5.2:+1491904 MS.gene21801:CDS
CGATGAGTTTGTGTGAATCG+AGG 0.648197 5.2:-1491693 None:intergenic
GTGAACGAGGATGCTGTGAA+CGG 0.695181 5.2:-1491644 None:intergenic
TAATGGCTTCGCGATCGTGG+TGG 0.707184 5.2:+1491609 MS.gene21801:CDS
ATGGACAGACACTTCCATAG+CGG 0.741310 5.2:+1494921 MS.gene21801:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGAAAAAAAATAAAAAG+GGG - chr5.2:1492960-1492979 None:intergenic 10.0%
!!! AATCTTTTTATTGATAATAA+AGG - chr5.2:1494501-1494520 None:intergenic 10.0%
!!! ATTAAAATAGTCATAATTTT+GGG - chr5.2:1493741-1493760 None:intergenic 10.0%
!!! TTATCAATAAAAAGATTTTT+TGG + chr5.2:1494504-1494523 MS.gene21801:intron 10.0%
!! AAATTAGGTAAACAAAAAAA+AGG - chr5.2:1491976-1491995 None:intergenic 15.0%
!! AATGTAAAGTGATTTATATT+TGG - chr5.2:1493588-1493607 None:intergenic 15.0%
!! AATTAGGTAAACAAAAAAAA+GGG - chr5.2:1491975-1491994 None:intergenic 15.0%
!!! AATAAAAAGATTTTTTGGAA+AGG + chr5.2:1494509-1494528 MS.gene21801:intron 15.0%
!!! ACAATTTTTATAACAAAGTT+TGG - chr5.2:1495023-1495042 None:intergenic 15.0%
!!! ATTAGGGTTTTAAAAAATTA+AGG - chr5.2:1492998-1493017 None:intergenic 15.0%
!!! CAAAATTATGACTATTTTAA+TGG + chr5.2:1493740-1493759 MS.gene21801:intron 15.0%
!!! CATTAAAATAGTCATAATTT+TGG - chr5.2:1493742-1493761 None:intergenic 15.0%
!!! TATGTTATTTATCAATAAGT+AGG + chr5.2:1494357-1494376 MS.gene21801:intron 15.0%
!!! TGTATTTTTTATTTCACTTT+TGG + chr5.2:1492106-1492125 MS.gene21801:intron 15.0%
!!! TTACTAGTACTATATAATTT+TGG + chr5.2:1493807-1493826 MS.gene21801:intron 15.0%
!! AATATACACAACCAATAATA+CGG - chr5.2:1493370-1493389 None:intergenic 20.0%
!! ATTAGGTAAACAAAAAAAAG+GGG - chr5.2:1491974-1491993 None:intergenic 20.0%
!! CTATTCAACACTAAATATTA+GGG - chr5.2:1492006-1492025 None:intergenic 20.0%
!! CTCATTTGTCTTAATTTATA+TGG + chr5.2:1494416-1494435 MS.gene21801:intron 20.0%
!! TAGATAATGATAGAACATTA+TGG - chr5.2:1493119-1493138 None:intergenic 20.0%
!! TATTAGGGTTTGAAAAAATT+AGG - chr5.2:1491991-1492010 None:intergenic 20.0%
!! TCTATTCAACACTAAATATT+AGG - chr5.2:1492007-1492026 None:intergenic 20.0%
!! TGTTATAAAAATTGTTGACA+AGG + chr5.2:1495028-1495047 MS.gene21801:CDS 20.0%
!!! AACACTAATCATCTTTTTAT+AGG + chr5.2:1494440-1494459 MS.gene21801:intron 20.0%
!!! ATAATTGATTTTGACATGTT+TGG + chr5.2:1493432-1493451 MS.gene21801:intron 20.0%
!!! ATATTTGGTTTTACTTTTAG+AGG + chr5.2:1493385-1493404 MS.gene21801:intron 20.0%
!!! ATTTTTATAACAAAGTTTGG+AGG - chr5.2:1495020-1495039 None:intergenic 20.0%
!!! ATTTTTTATTTCACTTTTGG+AGG + chr5.2:1492109-1492128 MS.gene21801:intron 20.0%
!!! GTAATTTTCCTTTGTTTTTT+TGG - chr5.2:1494164-1494183 None:intergenic 20.0%
!!! TATTATTGGTTGTGTATATT+TGG + chr5.2:1493370-1493389 MS.gene21801:intron 20.0%
! AAATACTGGATATATATGCT+TGG - chr5.2:1493928-1493947 None:intergenic 25.0%
! AAATAGGGTTTGAATGAAAT+GGG + chr5.2:1494319-1494338 MS.gene21801:intron 25.0%
! AATACTGGATATATATGCTT+GGG - chr5.2:1493927-1493946 None:intergenic 25.0%
! AATCTAAGTATATCAATAGC+TGG - chr5.2:1494673-1494692 None:intergenic 25.0%
! ACATACATAAACAATAATGC+AGG - chr5.2:1492341-1492360 None:intergenic 25.0%
! AGTCAATGTAATATAGTGAT+GGG - chr5.2:1494833-1494852 None:intergenic 25.0%
! AGTTTATTTACACGTGAATT+TGG + chr5.2:1494747-1494766 MS.gene21801:intron 25.0%
! ATAAAAATTGTTGACAAGGA+TGG + chr5.2:1495032-1495051 MS.gene21801:CDS 25.0%
! ATAAATTAAGACAAATGAGC+AGG - chr5.2:1494415-1494434 None:intergenic 25.0%
! ATATACACAACCAATAATAC+GGG - chr5.2:1493369-1493388 None:intergenic 25.0%
! GAAATATTACTAGTACTACT+TGG + chr5.2:1493766-1493785 MS.gene21801:intron 25.0%
! GTATAGATATATGTATGATG+AGG - chr5.2:1493037-1493056 None:intergenic 25.0%
! TAGTCAATGTAATATAGTGA+TGG - chr5.2:1494834-1494853 None:intergenic 25.0%
! TATTAAATACAATAAGTCGC+TGG - chr5.2:1492565-1492584 None:intergenic 25.0%
! TCTATAATAACAATGCAACA+TGG - chr5.2:1494722-1494741 None:intergenic 25.0%
! TTACATGAGAGATTTATGTT+TGG - chr5.2:1492294-1492313 None:intergenic 25.0%
! TTAGGTAAACAAAAAAAAGG+GGG - chr5.2:1491973-1491992 None:intergenic 25.0%
! TTTGAATGTATTCAGTAAAC+TGG - chr5.2:1491834-1491853 None:intergenic 25.0%
!! AATTCTTTTCGACAATTCTA+CGG - chr5.2:1494634-1494653 None:intergenic 25.0%
!! AATTGATTCTAGCTAAAAGT+AGG - chr5.2:1493614-1493633 None:intergenic 25.0%
!! AGCATATATATCCAGTATTT+TGG + chr5.2:1493928-1493947 MS.gene21801:intron 25.0%
!! GGATTTAGTTTATGTCAATT+GGG + chr5.2:1493204-1493223 MS.gene21801:intron 25.0%
!! TGGATTTAGTTTATGTCAAT+TGG + chr5.2:1493203-1493222 MS.gene21801:intron 25.0%
!! TTTGAGATTTGACAAGTATT+GGG + chr5.2:1492693-1492712 MS.gene21801:intron 25.0%
!!! AAGGATAGTACTGATTATTA+GGG - chr5.2:1493014-1493033 None:intergenic 25.0%
!!! AGATTTCAAGTAGTAGTTTT+CGG - chr5.2:1492848-1492867 None:intergenic 25.0%
!!! ATAATTGATTTTGACGTGTT+TGG + chr5.2:1492156-1492175 MS.gene21801:intron 25.0%
!!! CATCATTGATAGTGAAAAAT+AGG - chr5.2:1494904-1494923 None:intergenic 25.0%
!!! CTATTTTTCACTATCAATGA+TGG + chr5.2:1494902-1494921 MS.gene21801:CDS 25.0%
!!! GATTTTAGCCTTGAATTTAA+TGG + chr5.2:1493537-1493556 MS.gene21801:intron 25.0%
!!! GTACTATATAATTTTGGTCT+AGG + chr5.2:1493813-1493832 MS.gene21801:intron 25.0%
!!! TAATTGATTTTGACGTGTTT+GGG + chr5.2:1492157-1492176 MS.gene21801:intron 25.0%
!!! TTTTGAGATTTGACAAGTAT+TGG + chr5.2:1492692-1492711 MS.gene21801:intron 25.0%
!!! TTTTTTTGGAGTGATGATTA+TGG - chr5.2:1494150-1494169 None:intergenic 25.0%
AAAAAGGGGTAATTTGAACT+TGG - chr5.2:1492946-1492965 None:intergenic 30.0%
AAACACATGACAAGTTTAGA+TGG + chr5.2:1493276-1493295 MS.gene21801:intron 30.0%
AACACATGACAAGTTTAGAT+GGG + chr5.2:1493277-1493296 MS.gene21801:intron 30.0%
AACCTATGTCAAAAATCTCA+TGG + chr5.2:1492640-1492659 MS.gene21801:intron 30.0%
AAGGGTTTGAAAAAGTTTAC+AGG - chr5.2:1492594-1492613 None:intergenic 30.0%
AAGTTGAATCTCTTTCTTTC+TGG + chr5.2:1493688-1493707 MS.gene21801:intron 30.0%
ACTTGCAAAAATGGTTGAAT+GGG - chr5.2:1494220-1494239 None:intergenic 30.0%
AGATATATGTATGATGAGGA+AGG - chr5.2:1493033-1493052 None:intergenic 30.0%
ATATATGCTTGGGTTCATAT+GGG - chr5.2:1493917-1493936 None:intergenic 30.0%
CAGTTGAAGATGAAATAAAG+AGG + chr5.2:1492757-1492776 MS.gene21801:intron 30.0%
CTTTGTATCAATTCCGTAAT+GGG + chr5.2:1491863-1491882 MS.gene21801:CDS 30.0%
GAAATAGGGTTTGAATGAAA+TGG + chr5.2:1494318-1494337 MS.gene21801:intron 30.0%
GGATACATTTGTGTAAAAGT+GGG - chr5.2:1493567-1493586 None:intergenic 30.0%
TATATATGCTTGGGTTCATA+TGG - chr5.2:1493918-1493937 None:intergenic 30.0%
TATTTCTCTCAAAATTTGGG+GGG - chr5.2:1494304-1494323 None:intergenic 30.0%
TCATCACTCCAAAAAAACAA+AGG + chr5.2:1494153-1494172 MS.gene21801:intron 30.0%
TGGATACATTTGTGTAAAAG+TGG - chr5.2:1493568-1493587 None:intergenic 30.0%
! ACCCTATTTCTCTCAAAATT+TGG - chr5.2:1494308-1494327 None:intergenic 30.0%
! CCCTATTTCTCTCAAAATTT+GGG - chr5.2:1494307-1494326 None:intergenic 30.0%
! CCTATTTCTCTCAAAATTTG+GGG - chr5.2:1494306-1494325 None:intergenic 30.0%
! CTATTTCTCTCAAAATTTGG+GGG - chr5.2:1494305-1494324 None:intergenic 30.0%
! CTCTTTATTTCATCTTCAAC+TGG - chr5.2:1492759-1492778 None:intergenic 30.0%
! TACCATGAGATTTTTGACAT+AGG - chr5.2:1492645-1492664 None:intergenic 30.0%
! TTGAGATTTGACAAGTATTG+GGG + chr5.2:1492694-1492713 MS.gene21801:intron 30.0%
!! CATTCGTTTTTCACTTTTCA+GGG + chr5.2:1491807-1491826 MS.gene21801:intron 30.0%
!! CCCAAATTTTGAGAGAAATA+GGG + chr5.2:1494304-1494323 MS.gene21801:intron 30.0%
!! GAAGGATAGTACTGATTATT+AGG - chr5.2:1493015-1493034 None:intergenic 30.0%
!! TACACCTCTAGAATCAAATT+TGG - chr5.2:1493413-1493432 None:intergenic 30.0%
!! TCATTCGTTTTTCACTTTTC+AGG + chr5.2:1491806-1491825 MS.gene21801:intron 30.0%
!! TCTTGTTGATAAGTGCATTT+TGG + chr5.2:1494968-1494987 MS.gene21801:CDS 30.0%
!! TGTTTCTATCTCCTTTTGAA+CGG - chr5.2:1493261-1493280 None:intergenic 30.0%
!!! ATATCCAGTATTTTGGTTGA+GGG + chr5.2:1493935-1493954 MS.gene21801:intron 30.0%
!!! CATTAGATTTTTTACGCTAC+AGG + chr5.2:1494015-1494034 MS.gene21801:intron 30.0%
!!! TATATCCAGTATTTTGGTTG+AGG + chr5.2:1493934-1493953 MS.gene21801:intron 30.0%
AAAGCTACAAATCCTAGCTT+CGG - chr5.2:1492209-1492228 None:intergenic 35.0%
AAATAGGTTGTGGCTAATGA+AGG - chr5.2:1494124-1494143 None:intergenic 35.0%
AAGGGTTATCTCTCATACAA+TGG - chr5.2:1492797-1492816 None:intergenic 35.0%
AATAGGTTGTGGCTAATGAA+GGG - chr5.2:1494123-1494142 None:intergenic 35.0%
AATCTTGAAGCTTAGTGACA+GGG + chr5.2:1492664-1492683 MS.gene21801:intron 35.0%
ACAAATGAGCAGGAACTATT+AGG - chr5.2:1494405-1494424 None:intergenic 35.0%
ACACTTATGACTTACCTATC+CGG + chr5.2:1494600-1494619 MS.gene21801:intron 35.0%
ACACTTCACAAGCTTGAAAA+GGG + chr5.2:1494861-1494880 MS.gene21801:CDS 35.0%
AGAGATTCACAAACTGAAGT+AGG - chr5.2:1492732-1492751 None:intergenic 35.0%
AGGAATCTTAAAGGACTTTC+AGG + chr5.2:1493982-1494001 MS.gene21801:intron 35.0%
AGTCTGAGCCAAACTTAATA+AGG + chr5.2:1494237-1494256 MS.gene21801:intron 35.0%
ATGAGTTCGTTTGATCCATA+TGG + chr5.2:1492050-1492069 MS.gene21801:intron 35.0%
CAATTCTACGGATCAATATC+CGG - chr5.2:1494622-1494641 None:intergenic 35.0%
GACTTGCAAAAATGGTTGAA+TGG - chr5.2:1494221-1494240 None:intergenic 35.0%
GCTTTGTATCAATTCCGTAA+TGG + chr5.2:1491862-1491881 MS.gene21801:CDS 35.0%
GTAGATCAACAAGTGAAGAA+AGG + chr5.2:1492365-1492384 MS.gene21801:intron 35.0%
GTTCTGTTCAGGAATCTTAA+AGG + chr5.2:1493973-1493992 MS.gene21801:intron 35.0%
TAATCTTGAAGCTTAGTGAC+AGG + chr5.2:1492663-1492682 MS.gene21801:intron 35.0%
TCCCAAAACACATAAGAACA+CGG + chr5.2:1494066-1494085 MS.gene21801:intron 35.0%
TTAAGATTCCTGAACAGAAC+AGG - chr5.2:1493973-1493992 None:intergenic 35.0%
TTGTTTGATATGAAACCTGC+TGG - chr5.2:1492441-1492460 None:intergenic 35.0%
TTTGGAAATCAGATCAAGGT+TGG + chr5.2:1494986-1495005 MS.gene21801:CDS 35.0%
TTTGGCATGTCATTCTACAA+GGG - chr5.2:1492815-1492834 None:intergenic 35.0%
! ACATAGGTTTTCAGTTTCCA+GGG - chr5.2:1492629-1492648 None:intergenic 35.0%
! ACCGTGTTCTTATGTGTTTT+GGG - chr5.2:1494070-1494089 None:intergenic 35.0%
! ATTATGGTGGAAATAGGTTG+TGG - chr5.2:1494134-1494153 None:intergenic 35.0%
! GTGATGATTATGGTGGAAAT+AGG - chr5.2:1494140-1494159 None:intergenic 35.0%
! TCTTTTTCTGTGTATGTGAC+TGG - chr5.2:1494552-1494571 None:intergenic 35.0%
! TGGGTTGACCATTAAATTCA+AGG - chr5.2:1493548-1493567 None:intergenic 35.0%
!! ATTGATTTTAACCCGAAGCT+AGG + chr5.2:1492194-1492213 MS.gene21801:intron 35.0%
!! CCCCAAATTTTGAGAGAAAT+AGG + chr5.2:1494303-1494322 MS.gene21801:intron 35.0%
!! CTAAGTTAAGTTGATGATGG+AGG - chr5.2:1494099-1494118 None:intergenic 35.0%
!! GCATTTTGGAAATCAGATCA+AGG + chr5.2:1494982-1495001 MS.gene21801:CDS 35.0%
!! GGACTAAGTTAAGTTGATGA+TGG - chr5.2:1494102-1494121 None:intergenic 35.0%
!!! GCATTTTTTTTTCCCCATAC+TGG + chr5.2:1493144-1493163 MS.gene21801:intron 35.0%
!!! GTTTTCGGATTAGTTGGTAA+TGG + chr5.2:1491592-1491611 MS.gene21801:CDS 35.0%
!!! TATTTTGGTTGAGGGAAGTT+TGG + chr5.2:1493943-1493962 MS.gene21801:intron 35.0%
!!! TTTTGGAGTGATGATTATGG+TGG - chr5.2:1494147-1494166 None:intergenic 35.0%
AAACTTAATAAGGCTGCAGG+TGG + chr5.2:1494247-1494266 MS.gene21801:intron 40.0%
AAACTTTGGATGGGAAGAGA+TGG + chr5.2:1492896-1492915 MS.gene21801:intron 40.0%
AACTTAATAAGGCTGCAGGT+GGG + chr5.2:1494248-1494267 MS.gene21801:intron 40.0%
AACTTTGGATGGGAAGAGAT+GGG + chr5.2:1492897-1492916 MS.gene21801:intron 40.0%
AAGAAGGATGTTCACAGAGT+AGG - chr5.2:1494787-1494806 None:intergenic 40.0%
AAGCTACAAATCCTAGCTTC+GGG - chr5.2:1492208-1492227 None:intergenic 40.0%
AAGTCCTGTTTCAGTAAGTG+AGG - chr5.2:1492080-1492099 None:intergenic 40.0%
AATGAACAGATCACAGATGG+TGG - chr5.2:1494191-1494210 None:intergenic 40.0%
ACACAACCAATAATACGGGA+AGG - chr5.2:1493365-1493384 None:intergenic 40.0%
ACTTCCCTCAACCAAAATAC+TGG - chr5.2:1493942-1493961 None:intergenic 40.0%
ACTTTCATGTTAGTTCCAGC+AGG + chr5.2:1492423-1492442 MS.gene21801:intron 40.0%
AGAAGGATGTTCACAGAGTA+GGG - chr5.2:1494786-1494805 None:intergenic 40.0%
AGAATCCCTTCCCGTATTAT+TGG + chr5.2:1493356-1493375 MS.gene21801:intron 40.0%
ATCAAACAAACTCTGCCAGT+TGG + chr5.2:1492452-1492471 MS.gene21801:intron 40.0%
ATCAACCCATGAAACCAGTA+TGG - chr5.2:1493161-1493180 None:intergenic 40.0%
ATCCCTGTTATAGCCATTGT+AGG + chr5.2:1492389-1492408 MS.gene21801:intron 40.0%
ATCTCTTCCCATCCAAAGTT+TGG - chr5.2:1492897-1492916 None:intergenic 40.0%
ATTGAATACCTTGCGAAGAG+CGG - chr5.2:1491941-1491960 None:intergenic 40.0%
CACAACCAATAATACGGGAA+GGG - chr5.2:1493364-1493383 None:intergenic 40.0%
CACCTACAATGGCTATAACA+GGG - chr5.2:1492394-1492413 None:intergenic 40.0%
CAGCTAACCAAACTTTGGAT+GGG + chr5.2:1492887-1492906 MS.gene21801:intron 40.0%
CTTTGGCATGTCATTCTACA+AGG - chr5.2:1492816-1492835 None:intergenic 40.0%
CTTTGTCATAACCAGCAAGA+AGG - chr5.2:1494803-1494822 None:intergenic 40.0%
GAACATGATCTTGTCTTCGT+TGG - chr5.2:1491674-1491693 None:intergenic 40.0%
GACACTTCACAAGCTTGAAA+AGG + chr5.2:1494860-1494879 MS.gene21801:CDS 40.0%
GGAGCCAAATTTGATTCTAG+AGG + chr5.2:1493406-1493425 MS.gene21801:intron 40.0%
GTCAATGAACAGATCACAGA+TGG - chr5.2:1494194-1494213 None:intergenic 40.0%
TAACCCAGCTAACCAAACTT+TGG + chr5.2:1492882-1492901 MS.gene21801:intron 40.0%
TAGTTGACATGCGAATGGTT+TGG + chr5.2:1492475-1492494 MS.gene21801:intron 40.0%
TCAACCCATGAAACCAGTAT+GGG - chr5.2:1493160-1493179 None:intergenic 40.0%
TCACCTACAATGGCTATAAC+AGG - chr5.2:1492395-1492414 None:intergenic 40.0%
TCACTTACTGAAACAGGACT+TGG + chr5.2:1492079-1492098 MS.gene21801:intron 40.0%
TTCAGTTTCCAGGGTTATTG+AGG - chr5.2:1492620-1492639 None:intergenic 40.0%
TTGGCTCAGACTTGCAAAAA+TGG - chr5.2:1494229-1494248 None:intergenic 40.0%
! AAGCTTGAAAAGGGAGCTTT+TGG + chr5.2:1494870-1494889 MS.gene21801:CDS 40.0%
! AATCAGATCAAGGTTGGTTG+TGG + chr5.2:1494992-1495011 MS.gene21801:CDS 40.0%
! CACCGTGTTCTTATGTGTTT+TGG - chr5.2:1494071-1494090 None:intergenic 40.0%
! CATCCAAAGTTTGGTTAGCT+GGG - chr5.2:1492888-1492907 None:intergenic 40.0%
! CTTTCTTTTACTGTCAGTGC+TGG + chr5.2:1494473-1494492 MS.gene21801:intron 40.0%
! GACATAGGTTTTCAGTTTCC+AGG - chr5.2:1492630-1492649 None:intergenic 40.0%
! GGATACTTTTGTGCTAGAGT+GGG - chr5.2:1492273-1492292 None:intergenic 40.0%
! TGAATCTCTTTCTTTCTGGC+TGG + chr5.2:1493692-1493711 MS.gene21801:intron 40.0%
! TGGATACTTTTGTGCTAGAG+TGG - chr5.2:1492274-1492293 None:intergenic 40.0%
!! ACCTGCAGCCTTATTAAGTT+TGG - chr5.2:1494248-1494267 None:intergenic 40.0%
!! GAAAAGGGAGCTTTTGGTTA+TGG + chr5.2:1494876-1494895 MS.gene21801:CDS 40.0%
!! TTGACAAGTATTGGGGTTAG+TGG + chr5.2:1492701-1492720 MS.gene21801:intron 40.0%
!! TTGGTTTTCCTGTTCTGTTC+AGG + chr5.2:1493962-1493981 MS.gene21801:intron 40.0%
!! TTTCCCCATACTGGTTTCAT+GGG + chr5.2:1493153-1493172 MS.gene21801:intron 40.0%
!!! GAATGCGTTTTCGGATTAGT+TGG + chr5.2:1491586-1491605 MS.gene21801:CDS 40.0%
!!! TTTTCCCCATACTGGTTTCA+TGG + chr5.2:1493152-1493171 MS.gene21801:intron 40.0%
AAGAGAGTGAAACCTGTCAC+CGG - chr5.2:1491734-1491753 None:intergenic 45.0%
AAGCCTTAACCCACTAAGTG+GGG + chr5.2:1493070-1493089 MS.gene21801:intron 45.0%
ACTTAATAAGGCTGCAGGTG+GGG + chr5.2:1494249-1494268 MS.gene21801:intron 45.0%
ATGGACAGACACTTCCATAG+CGG + chr5.2:1494921-1494940 MS.gene21801:CDS 45.0%
CAACCCATGAAACCAGTATG+GGG - chr5.2:1493159-1493178 None:intergenic 45.0%
CAAGCCTTAACCCACTAAGT+GGG + chr5.2:1493069-1493088 MS.gene21801:intron 45.0%
CCAGCTAACCAAACTTTGGA+TGG + chr5.2:1492886-1492905 MS.gene21801:intron 45.0%
CCAGGGTTATTGAGGTTCAA+GGG - chr5.2:1492612-1492631 None:intergenic 45.0%
CCATCCAAAGTTTGGTTAGC+TGG - chr5.2:1492889-1492908 None:intergenic 45.0%
CCTGTTTCAGTAAGTGAGGT+CGG - chr5.2:1492076-1492095 None:intergenic 45.0%
CGATGAGTTTGTGTGAATCG+AGG - chr5.2:1491696-1491715 None:intergenic 45.0%
CTACGGATCAATATCCGGAT+AGG - chr5.2:1494617-1494636 None:intergenic 45.0%
CTTCGTTGGATTTGTGAACG+AGG - chr5.2:1491660-1491679 None:intergenic 45.0%
GAATAGTCTAAGCGTGCGTT+TGG - chr5.2:1493666-1493685 None:intergenic 45.0%
GCCAAACTTAATAAGGCTGC+AGG + chr5.2:1494244-1494263 MS.gene21801:intron 45.0%
GCTGGTTATGACAAAGAGAC+AGG + chr5.2:1494807-1494826 MS.gene21801:CDS 45.0%
GTCATAAGTGTGCTGTGTCA+TGG - chr5.2:1494591-1494610 None:intergenic 45.0%
TCAAGCCTTAACCCACTAAG+TGG + chr5.2:1493068-1493087 MS.gene21801:intron 45.0%
TCCAGGGTTATTGAGGTTCA+AGG - chr5.2:1492613-1492632 None:intergenic 45.0%
TCTGTGAACATCCTTCTTGC+TGG + chr5.2:1494789-1494808 MS.gene21801:CDS 45.0%
TGGTGAGAGGAATCCCATTA+CGG - chr5.2:1491879-1491898 None:intergenic 45.0%
TTCGCATGTCAACTACCAAC+TGG - chr5.2:1492470-1492489 None:intergenic 45.0%
! ATGGTGCAAGAGAGTATGCA+TGG + chr5.2:1495051-1495070 MS.gene21801:CDS 45.0%
! GGTGTCGAGATTGATTCTAG+AGG + chr5.2:1492130-1492149 MS.gene21801:intron 45.0%
! GTTTTCGGCTCTCTCAACTT+TGG - chr5.2:1492833-1492852 None:intergenic 45.0%
!! ATCTCTTTCTTTCTGGCTGG+AGG + chr5.2:1493695-1493714 MS.gene21801:intron 45.0%
!! GTTGGTAGTTGACATGCGAA+TGG + chr5.2:1492470-1492489 MS.gene21801:intron 45.0%
!! AAATTAGAAAAAAAATAAAA+AGG - chr5.2:1492962-1492981 None:intergenic 5.0%
!! AATGTTAAAAATTTAATATT+TGG + chr5.2:1493183-1493202 MS.gene21801:intron 5.0%
!! AATTAGAAAAAAAATAAAAA+GGG - chr5.2:1492961-1492980 None:intergenic 5.0%
AGAGAGTGAAACCTGTCACC+GGG - chr5.2:1491733-1491752 None:intergenic 50.0%
AGTAAGTGAGGTCGGCCATA+TGG - chr5.2:1492068-1492087 None:intergenic 50.0%
AGTGTGCTGTGTCATGGTGA+TGG - chr5.2:1494585-1494604 None:intergenic 50.0%
ATGTATCCGACCCCACTTAG+TGG - chr5.2:1493083-1493102 None:intergenic 50.0%
CACAAACTCATCGCTGCTAG+CGG + chr5.2:1491703-1491722 MS.gene21801:CDS 50.0%
CCCTTGAACCTCAATAACCC+TGG + chr5.2:1492609-1492628 MS.gene21801:intron 50.0%
CCGACCTCACTTACTGAAAC+AGG + chr5.2:1492073-1492092 MS.gene21801:intron 50.0%
CTCGCCACGAGTGAAATTAG+CGG - chr5.2:1491911-1491930 None:intergenic 50.0%
CTTAACCCACTAAGTGGGGT+CGG + chr5.2:1493074-1493093 MS.gene21801:intron 50.0%
CTTCCACGTTCATTCCGCTA+TGG - chr5.2:1494938-1494957 None:intergenic 50.0%
GGATACCATCATCTCTCGCT+CGG - chr5.2:1492925-1492944 None:intergenic 50.0%
GTGAACGAGGATGCTGTGAA+CGG - chr5.2:1491647-1491666 None:intergenic 50.0%
GTGACTGCTCTCACCTACAA+TGG - chr5.2:1492405-1492424 None:intergenic 50.0%
GTGATGTCTCGCCGTTCAAA+AGG + chr5.2:1493247-1493266 MS.gene21801:intron 50.0%
GTGTGCTGTGTCATGGTGAT+GGG - chr5.2:1494584-1494603 None:intergenic 50.0%
TGTATCCGACCCCACTTAGT+GGG - chr5.2:1493082-1493101 None:intergenic 50.0%
! CTTCCATAGCGGAATGAACG+TGG + chr5.2:1494932-1494951 MS.gene21801:CDS 50.0%
! TGTGCTGTGTCATGGTGATG+GGG - chr5.2:1494583-1494602 None:intergenic 50.0%
GAAATTAGCGGCAGCAGCTG+TGG - chr5.2:1491899-1491918 None:intergenic 55.0%
GAATACCTTGCGAAGAGCGG+TGG - chr5.2:1491938-1491957 None:intergenic 55.0%
GCTGCCGCTAATTTCACTCG+TGG + chr5.2:1491904-1491923 MS.gene21801:CDS 55.0%
TAATGGCTTCGCGATCGTGG+TGG + chr5.2:1491609-1491628 MS.gene21801:CDS 55.0%
TGCATGGCGCGAATCAGTCA+AGG + chr5.2:1495067-1495086 MS.gene21801:CDS 55.0%
!! CGACCCCACTTAGTGGGTTA+AGG - chr5.2:1493076-1493095 None:intergenic 55.0%
!! GTGCTGTGTCATGGTGATGG+GGG - chr5.2:1494582-1494601 None:intergenic 55.0%
!! TGGTAATGGCTTCGCGATCG+TGG + chr5.2:1491606-1491625 MS.gene21801:CDS 55.0%
ATCGCTGCTAGCGGTGAACC+CGG + chr5.2:1491712-1491731 MS.gene21801:CDS 60.0%
GGGAACCGAGCGAGAGATGA+TGG + chr5.2:1492917-1492936 MS.gene21801:intron 60.0%
TAAGGCTGCAGGTGGGGTAC+TGG + chr5.2:1494255-1494274 MS.gene21801:intron 60.0%
!! GTGGGGTACTGGTGGTGGAA+GGG + chr5.2:1494266-1494285 MS.gene21801:intron 60.0%
CTAGCGGTGAACCCGGTGAC+AGG + chr5.2:1491719-1491738 MS.gene21801:CDS 65.0%
GCTTGCCACCGCTCTTCGCA+AGG + chr5.2:1491930-1491949 MS.gene21801:CDS 65.0%
!! GGTGGGGTACTGGTGGTGGA+AGG + chr5.2:1494265-1494284 MS.gene21801:intron 65.0%
!! TGCAGGTGGGGTACTGGTGG+TGG + chr5.2:1494261-1494280 MS.gene21801:intron 65.0%
GCGGCAGCAGCTGTGGTGAG+AGG - chr5.2:1491892-1491911 None:intergenic 70.0%
!! GGCTGCAGGTGGGGTACTGG+TGG + chr5.2:1494258-1494277 MS.gene21801:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 1491583 1495109 1491583 ID=MS.gene21801
chr5.2 mRNA 1491583 1495109 1491583 ID=MS.gene21801.t1;Parent=MS.gene21801
chr5.2 exon 1491583 1491740 1491583 ID=MS.gene21801.t1.exon1;Parent=MS.gene21801.t1
chr5.2 CDS 1491583 1491740 1491583 ID=cds.MS.gene21801.t1;Parent=MS.gene21801.t1
chr5.2 exon 1491828 1491951 1491828 ID=MS.gene21801.t1.exon2;Parent=MS.gene21801.t1
chr5.2 CDS 1491828 1491951 1491828 ID=cds.MS.gene21801.t1;Parent=MS.gene21801.t1
chr5.2 exon 1494780 1495109 1494780 ID=MS.gene21801.t1.exon3;Parent=MS.gene21801.t1
chr5.2 CDS 1494780 1495109 1494780 ID=cds.MS.gene21801.t1;Parent=MS.gene21801.t1
Gene Sequence

>MS.gene21801

ATGGAATGCGTTTTCGGATTAGTTGGTAATGGCTTCGCGATCGTGGTGGCTGATACATCTGCCGTTCACAGCATCCTCGTTCACAAATCCAACGAAGACAAGATCATGTTCCTCGATTCACACAAACTCATCGCTGCTAGCGGTGAACCCGGTGACAGGTTTCACTCTCTTTCATCATCTTCTTCTTTTTCTCTTTGTAACTAATTTTGATTTTGATTTTGATTCATTCGTTTTTCACTTTTCAGGGTCCAGTTTACTGAATACATTCAAAAGAACGTTGCTTTGTATCAATTCCGTAATGGGATTCCTCTCACCACAGCTGCTGCCGCTAATTTCACTCGTGGCGAGCTTGCCACCGCTCTTCGCAAGGTATTCAATTTCTAAATACCCCCTTTTTTTTGTTTACCTAATTTTTTCAAACCCTAATATTTAGTGTTGAATAGAGATATTGTATGTGATAGAATATCATGAGTTCGTTTGATCCATATGGCCGACCTCACTTACTGAAACAGGACTTGGTTGTTGTATTTTTTATTTCACTTTTGGAGGTGTCGAGATTGATTCTAGAGGTGTATAATTGATTTTGACGTGTTTGGGTGTTCTGAAGTAGAATTGATTTTAACCCGAAGCTAGGATTTGTAGCTTTTGACTTCTATAATTGATTTTAGACTTGAATTTAATGTTCAGCCCACTCTAGCACAAAAGTATCCAAACATAAATCTCTCATGTAAAAATCACATTTTAGAGAATACACTCCTGCATTATTGTTTATGTATGTGAGAGTAGATCAACAAGTGAAGAAAGGCATCCCTGTTATAGCCATTGTAGGTGAGAGCAGTCACTTTCATGTTAGTTCCAGCAGGTTTCATATCAAACAAACTCTGCCAGTTGGTAGTTGACATGCGAATGGTTTGGTTATTCAGTTTGAACAAGAATTTCTCACGTATAACAGATGCGAACACACAGTAAAATGCTCTCACCAGCGACTTATTGTATTTAATATTCAGACCTGTAAACTTTTTCAAACCCTTGAACCTCAATAACCCTGGAAACTGAAAACCTATGTCAAAAATCTCATGGTAATCTTGAAGCTTAGTGACAGGGAGAAATTTTGAGATTTGACAAGTATTGGGGTTAGTGGAATCACCTACTTCAGTTTGTGAATCTCTATTACCAGTTGAAGATGAAATAAAGAGGAGAATTGATAAATACCATTGTATGAGAGATAACCCTTGTAGAATGACATGCCAAAGTTGAGAGAGCCGAAAACTACTACTTGAAATCTGAATTGACGAGTAGTAACCCAGCTAACCAAACTTTGGATGGGAAGAGATGGGAACCGAGCGAGAGATGATGGTATCCAAGTTCAAATTACCCCTTTTTATTTTTTTTCTAATTTTTCTTCTTGTTTACCTTAATTTTTTAAAACCCTAATAATCAGTACTATCCTTCCTCATCATACATATATCTATACAACAGCAACAATCAAGCCTTAACCCACTAAGTGGGGTCGGATACATTGATCAAATTACGCCATAATGTTCTATCATTATCTATAATTGCATTTTTTTTTCCCCATACTGGTTTCATGGGTTGATCTAATGTTAAAAATTTAATATTTGGATTTAGTTTATGTCAATTGGGATTTTTTGTACACAAAAGATGTGATGTCTCGCCGTTCAAAAGGAGATAGAAACACATGACAAGTTTAGATGGGATGAATTTTAATAGTGAGAGATCACACTTTACTCTCACGTGAAAATTACACTTAAGAGAATCCCTTCCCGTATTATTGGTTGTGTATATTTGGTTTTACTTTTAGAGGAGCCAAATTTGATTCTAGAGGTGTATAATTGATTTTGACATGTTTGGATGTTCTCAAGTAGAATTGATTTTACTTTTAGAATTGATTTTAATTCGAAGTTATGATTTGTAGCTTTTGACTTCTATAATTGATTTTAGCCTTGAATTTAATGGTCAACCCACTTTTACACAAATGTATCCAAATATAAATCACTTTACATTCAACCTACTTTTAGCTAGAATCAATTTTACAAAATTAATTTATGTCGCTGTAGAACCAAACGCACGCTTAGACTATTCTAAAGTTGAATCTCTTTCTTTCTGGCTGGAGGATAGATCTATAAATAAGTAACCCAAAATTATGACTATTTTAATGGATAGAAATATTACTAGTACTACTTGGTTTTTTTTTTGTTGTTTCTTACTAGTACTATATAATTTTGGTCTAGGTGATGAGATGAATATGAACTTGTAAGTTGTAGCTACTGCATCTCTTGTTCGTTTATGTTTGACTTACAACAGAACTGACCCATATGAACCCAAGCATATATATCCAGTATTTTGGTTGAGGGAAGTTTGGTTTTCCTGTTCTGTTCAGGAATCTTAAAGGACTTTCAGGATTATCGTATCATTAGATTTTTTACGCTACAGGTTAATGTTTTTTTTTTTTAATAAAACTATCCCAAAACACATAAGAACACGGTGTGCAACCTCCATCATCAACTTAACTTAGTCCCTTCATTAGCCACAACCTATTTCCACCATAATCATCACTCCAAAAAAACAAAGGAAAATTACTGTGCCACCATCTGTGATCTGTTCATTGACTTGCCCATTCAACCATTTTTGCAAGTCTGAGCCAAACTTAATAAGGCTGCAGGTGGGGTACTGGTGGTGGAAGGGAATGAGAGACATCCCCCCAAATTTTGAGAGAAATAGGGTTTGAATGAAATGGGAATTAAACTTTTGATTATGTTATTTATCAATAAGTAGGTGTCTGAAAAAAATTGTGATATCCTAATAGTTCCTGCTCATTTGTCTTAATTTATATGGCAACACTAATCATCTTTTTATAGGACTTTCTCTTCTTTCTTTTACTGTCAGTGCTGGAACCTTTATTATCAATAAAAAGATTTTTTGGAAAGGAAATGACTAGTGAACTTCCAGTCACATACACAGAAAAAGAATATCGTCCCCCATCACCATGACACAGCACACTTATGACTTACCTATCCGGATATTGATCCGTAGAATTGTCGAAAAGAATTGAAATATTTTATAAATCCAGCTATTGATATACTTAGATTTATGTTGTTTTGCAGCAATTCATTAACCATGTTGCATTGTTATTATAGATAACGAGTTTATTTACACGTGAATTTGGTTTTCTTCAGAATCCCTACTCTGTGAACATCCTTCTTGCTGGTTATGACAAAGAGACAGGCCCATCACTATATTACATTGACTACATTGCGACACTTCACAAGCTTGAAAAGGGAGCTTTTGGTTATGGTTCCTATTTTTCACTATCAATGATGGACAGACACTTCCATAGCGGAATGAACGTGGAAGAAGCAATTGATCTTGTTGATAAGTGCATTTTGGAAATCAGATCAAGGTTGGTTGTGGCACCTCCAAACTTTGTTATAAAAATTGTTGACAAGGATGGTGCAAGAGAGTATGCATGGCGCGAATCAGTCAAGGACACTCCTGCTTCAGCTTGA
Protein sequence

>MS.gene21801.t1

MECVFGLVGNGFAIVVADTSAVHSILVHKSNEDKIMFLDSHKLIAASGEPGDRVQFTEYIQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMNVEEAIDLVDKCILEIRSRLVVAPPNFVIKIVDKDGAREYAWRESVKDTPASA