AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071036


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071036.t1 MTR_7g070070 98.800 250 3 0 1 250 1 250 0.0 511
MS.gene071036.t1 MTR_5g016590 97.600 250 6 0 1 250 1 250 0.0 506
MS.gene071036.t1 MTR_1g112250 44.700 217 108 5 5 215 8 218 2.14e-57 183
MS.gene071036.t1 MTR_8g075320 42.000 200 113 3 4 202 3 200 1.30e-52 171
MS.gene071036.t1 MTR_2g016050 41.000 200 115 3 4 202 3 200 2.51e-50 165
MS.gene071036.t1 MTR_0167s0060 38.095 210 127 3 4 213 5 211 7.21e-47 156
MS.gene071036.t1 MTR_8g464460 37.712 236 139 4 5 237 9 239 1.42e-43 147
MS.gene071036.t1 MTR_1g022410 38.785 214 125 3 4 215 5 214 1.76e-43 149
MS.gene071036.t1 MTR_2g060830 37.395 238 137 6 5 237 9 239 1.15e-41 143
MS.gene071036.t1 MTR_6g024135 35.071 211 135 2 5 215 8 216 2.37e-37 132
MS.gene071036.t1 MTR_4g012740 28.968 252 135 6 4 215 236 483 4.51e-22 95.1
MS.gene071036.t1 MTR_6g013300 32.105 190 112 7 33 215 138 317 8.91e-16 75.9
MS.gene071036.t1 MTR_4g057090 29.947 187 119 5 26 207 110 289 1.92e-14 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071036.t1 AT3G22110 89.600 250 26 0 1 250 1 250 6.65e-172 474
MS.gene071036.t1 AT4G15165 71.600 250 29 1 1 250 1 208 3.76e-126 356
MS.gene071036.t1 AT3G14290 44.495 218 109 5 4 215 7 218 1.33e-57 183
MS.gene071036.t1 AT1G53850 43.578 218 111 5 4 215 7 218 1.25e-56 181
MS.gene071036.t1 AT1G53850 43.578 218 111 5 4 215 7 218 1.25e-56 181
MS.gene071036.t1 AT5G66140 41.500 200 114 3 4 202 3 200 4.76e-52 169
MS.gene071036.t1 AT3G51260 42.000 200 113 3 4 202 3 200 6.46e-52 169
MS.gene071036.t1 AT3G51260 42.000 200 113 3 4 202 3 200 6.58e-52 169
MS.gene071036.t1 AT1G16470 36.792 212 131 3 4 215 5 213 5.55e-46 154
MS.gene071036.t1 AT1G16470 36.792 212 131 3 4 215 5 213 5.55e-46 154
MS.gene071036.t1 AT1G79210 36.792 212 131 3 4 215 5 213 6.12e-46 153
MS.gene071036.t1 AT1G79210 36.792 212 131 3 4 215 5 213 6.12e-46 153
MS.gene071036.t1 AT1G79210 36.792 212 131 3 4 215 5 213 6.12e-46 153
MS.gene071036.t1 AT5G42790 39.320 206 119 3 4 207 5 206 2.58e-44 150
MS.gene071036.t1 AT1G47250 38.350 206 121 3 4 207 5 206 1.49e-43 149
MS.gene071036.t1 AT5G35590 40.201 199 115 3 5 200 9 206 1.78e-41 142
MS.gene071036.t1 AT2G05840 37.688 199 120 3 5 200 9 206 3.33e-38 133
MS.gene071036.t1 AT2G05840 37.688 199 120 3 5 200 9 206 4.68e-38 134
MS.gene071036.t1 AT2G05840 37.745 204 116 4 5 205 9 204 2.17e-37 130
MS.gene071036.t1 AT2G27020 33.493 209 137 2 5 213 8 214 4.78e-36 128
MS.gene071036.t1 AT2G27020 33.175 211 139 2 5 215 110 318 6.07e-35 128

Find 62 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTCTCTTTCCTATTTGC+AGG 0.250981 7.3:+34848034 MS.gene071036:CDS
GTCAAAAGACGGGGTTGTTC+TGG 0.306994 7.3:+34847757 MS.gene071036:CDS
ACTAAGCTATTGGTGAAGTC+TGG 0.325538 7.3:+34848343 MS.gene071036:CDS
CTCTTTCCTATTTGCAGGCT+GGG 0.331080 7.3:+34848039 MS.gene071036:CDS
AGCAGTTCAACTTGCATTGA+AGG 0.356948 7.3:+34848204 MS.gene071036:CDS
TGCAGTGTCTGTTGCTGGTT+GGG 0.362610 7.3:-34848371 None:intergenic
GGATCCTAGCAGTATTGATT+AGG 0.366704 7.3:-34847898 None:intergenic
TTCGGTGGTCTCCGACCATT+TGG 0.375556 7.3:+34848013 MS.gene071036:CDS
AGAGAATTTGACTAAGCTAT+TGG 0.375935 7.3:+34848333 MS.gene071036:CDS
GATTGAACCAACTGCTCAAC+AGG 0.380070 7.3:-34847959 None:intergenic
TCTCTTTCCTATTTGCAGGC+TGG 0.384561 7.3:+34848038 MS.gene071036:CDS
AACTATGCAGTGTCTGTTGC+TGG 0.401373 7.3:-34848376 None:intergenic
GAAGGTTCTGAGTAAAACTA+TGG 0.403623 7.3:+34848222 MS.gene071036:CDS
GGTTGGAAAGCTGGTGCTAT+TGG 0.408203 7.3:+34848115 MS.gene071036:CDS
GGAAATGCTGGTACTGCAAT+AGG 0.415260 7.3:+34847728 MS.gene071036:CDS
TGACTGTGCTGCCTGGTTGT+TGG 0.416766 7.3:-34848140 None:intergenic
AGTAATCCAAGCGGAAACTA+TGG 0.425766 7.3:+34848091 MS.gene071036:CDS
CATGTAGCATGTGCTGTCGC+TGG 0.433619 7.3:+34847854 MS.gene071036:CDS
AGCACAGTCAATTCTAAAAC+AGG 0.450248 7.3:+34848153 MS.gene071036:CDS
GCTCAACAGGCATTGGCTCT+TGG 0.472402 7.3:-34847946 None:intergenic
TTAGAATTGACTGTGCTGCC+TGG 0.476900 7.3:-34848147 None:intergenic
AACTATGGTGGTTGGAAAGC+TGG 0.479761 7.3:+34848106 MS.gene071036:CDS
GCCAATGCCTGTTGAGCAGT+TGG 0.481970 7.3:+34847952 MS.gene071036:CDS
ACTTGGTACAGACGTCCTTC+AGG 0.486610 7.3:-34847683 None:intergenic
AAGCAAGGATACACACAATT+CGG 0.488228 7.3:+34847995 MS.gene071036:CDS
ATGCAGTGTCTGTTGCTGGT+TGG 0.488987 7.3:-34848372 None:intergenic
CTAAGCTATTGGTGAAGTCT+GGG 0.494770 7.3:+34848344 MS.gene071036:CDS
AGCAGTATTGATTAGGATGT+TGG 0.498265 7.3:-34847891 None:intergenic
TAGGAATCTTGTCAAAAGAC+GGG 0.510970 7.3:+34847747 MS.gene071036:CDS
CAAAGGAGTAACGTTGTGCT+TGG 0.511294 7.3:-34847919 None:intergenic
ATGGAGGCCATCGGAAATGC+TGG 0.514078 7.3:+34847716 MS.gene071036:CDS
CAACCACCATAGTTTCCGCT+TGG 0.517588 7.3:-34848097 None:intergenic
ACCAACTGCTCAACAGGCAT+TGG 0.521861 7.3:-34847953 None:intergenic
CAGGAGAGAATATTGTTGTA+CGG 0.539096 7.3:-34847664 None:intergenic
TGCTATTGGTGCCAACAACC+AGG 0.541336 7.3:+34848129 MS.gene071036:CDS
TGTTCTGGTTGGTGAAAAGA+AGG 0.542861 7.3:+34847772 MS.gene071036:CDS
CAAGCGGAAACTATGGTGGT+TGG 0.545365 7.3:+34848098 MS.gene071036:CDS
AGTTGAGGTTTGAAGCAGCT+TGG 0.546459 7.3:-34847801 None:intergenic
ATGTAGCATGTGCTGTCGCT+GGG 0.546740 7.3:+34847855 MS.gene071036:CDS
AATTCTAAAACAGGACTACA+AGG 0.553889 7.3:+34848162 MS.gene071036:CDS
CCTCCATTGCATACTCCACT+TGG 0.562163 7.3:-34847700 None:intergenic
ATAGGAATCTTGTCAAAAGA+CGG 0.567737 7.3:+34847746 MS.gene071036:CDS
AAAGACGGGGTTGTTCTGGT+TGG 0.578404 7.3:+34847761 MS.gene071036:CDS
ACATCCTAATCAATACTGCT+AGG 0.578961 7.3:+34847894 MS.gene071036:CDS
AATAGGAAAGAGACTCCAAA+TGG 0.587032 7.3:-34848028 None:intergenic
CCAAGTGGAGTATGCAATGG+AGG 0.592642 7.3:+34847700 MS.gene071036:CDS
CATTGGCTCTTGGTAAGCAA+AGG 0.596164 7.3:-34847936 None:intergenic
TGCAGTACCAGCATTTCCGA+TGG 0.599600 7.3:-34847723 None:intergenic
GTACCAAGTGGAGTATGCAA+TGG 0.605368 7.3:+34847697 MS.gene071036:CDS
ACAACAATATTCTCTCCTGA+AGG 0.618706 7.3:+34847668 MS.gene071036:CDS
GGAAAGAGACTCCAAATGGT+CGG 0.621181 7.3:-34848024 None:intergenic
GAGTATGCAATGGAGGCCAT+CGG 0.626386 7.3:+34847707 MS.gene071036:CDS
TCTCTCTGTGATACTAAGCA+AGG 0.640603 7.3:+34847980 MS.gene071036:CDS
AATCCAAGCGGAAACTATGG+TGG 0.659270 7.3:+34848094 MS.gene071036:CDS
CAAGGATACACACAATTCGG+TGG 0.675083 7.3:+34847998 MS.gene071036:CDS
TGAAGGACGTCTGTACCAAG+TGG 0.675276 7.3:+34847685 MS.gene071036:CDS
AGGAATCTTGTCAAAAGACG+GGG 0.680553 7.3:+34847748 MS.gene071036:CDS
ACTACAAGGATGATATCACA+AGG 0.683851 7.3:+34848176 MS.gene071036:CDS
TAGCTTAGTCAAATTCTCTG+AGG 0.695782 7.3:-34848329 None:intergenic
TAAGCTATTGGTGAAGTCTG+GGG 0.701098 7.3:+34848345 MS.gene071036:CDS
CTTTACATGAGTAATCCAAG+CGG 0.723308 7.3:+34848082 MS.gene071036:CDS
CTACAAGGATGATATCACAA+GGG 0.766354 7.3:+34848177 MS.gene071036:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAGAATTTGACTAAGCTAT+TGG + chr7.3:34848333-34848352 MS.gene071036:CDS 30.0%
ATAGGAATCTTGTCAAAAGA+CGG + chr7.3:34847746-34847765 MS.gene071036:CDS 30.0%
!! AATTCTAAAACAGGACTACA+AGG + chr7.3:34848162-34848181 MS.gene071036:CDS 30.0%
AAGCAAGGATACACACAATT+CGG + chr7.3:34847995-34848014 MS.gene071036:CDS 35.0%
AATAGGAAAGAGACTCCAAA+TGG - chr7.3:34848031-34848050 None:intergenic 35.0%
ACAACAATATTCTCTCCTGA+AGG + chr7.3:34847668-34847687 MS.gene071036:CDS 35.0%
ACATCCTAATCAATACTGCT+AGG + chr7.3:34847894-34847913 MS.gene071036:CDS 35.0%
ACTACAAGGATGATATCACA+AGG + chr7.3:34848176-34848195 MS.gene071036:CDS 35.0%
CAGGAGAGAATATTGTTGTA+CGG - chr7.3:34847667-34847686 None:intergenic 35.0%
CTACAAGGATGATATCACAA+GGG + chr7.3:34848177-34848196 MS.gene071036:CDS 35.0%
CTTTACATGAGTAATCCAAG+CGG + chr7.3:34848082-34848101 MS.gene071036:CDS 35.0%
TAGCTTAGTCAAATTCTCTG+AGG - chr7.3:34848332-34848351 None:intergenic 35.0%
TAGGAATCTTGTCAAAAGAC+GGG + chr7.3:34847747-34847766 MS.gene071036:CDS 35.0%
! CATTTTCTCAGTTGAAGTTG+AGG - chr7.3:34847819-34847838 None:intergenic 35.0%
! TGATACTTGACTTTTCCAGA+GGG - chr7.3:34848301-34848320 None:intergenic 35.0%
! TTGATACTTGACTTTTCCAG+AGG - chr7.3:34848302-34848321 None:intergenic 35.0%
!! AGCACAGTCAATTCTAAAAC+AGG + chr7.3:34848153-34848172 MS.gene071036:CDS 35.0%
!! AGCAGTATTGATTAGGATGT+TGG - chr7.3:34847894-34847913 None:intergenic 35.0%
!! GAAGGTTCTGAGTAAAACTA+TGG + chr7.3:34848222-34848241 MS.gene071036:CDS 35.0%
ACTAAGCTATTGGTGAAGTC+TGG + chr7.3:34848343-34848362 MS.gene071036:CDS 40.0%
AGCAGTTCAACTTGCATTGA+AGG + chr7.3:34848204-34848223 MS.gene071036:CDS 40.0%
AGGAATCTTGTCAAAAGACG+GGG + chr7.3:34847748-34847767 MS.gene071036:CDS 40.0%
AGTAATCCAAGCGGAAACTA+TGG + chr7.3:34848091-34848110 MS.gene071036:CDS 40.0%
CTAAGCTATTGGTGAAGTCT+GGG + chr7.3:34848344-34848363 MS.gene071036:CDS 40.0%
TAAGCTATTGGTGAAGTCTG+GGG + chr7.3:34848345-34848364 MS.gene071036:CDS 40.0%
TCTCTCTGTGATACTAAGCA+AGG + chr7.3:34847980-34847999 MS.gene071036:CDS 40.0%
! GAAGTTTTTCTTTCACCCTC+TGG + chr7.3:34848283-34848302 MS.gene071036:CDS 40.0%
! TGCAAGTTCAAGCTTTTCTG+AGG - chr7.3:34848263-34848282 None:intergenic 40.0%
!! GGATCCTAGCAGTATTGATT+AGG - chr7.3:34847901-34847920 None:intergenic 40.0%
!! TGTTCTGGTTGGTGAAAAGA+AGG + chr7.3:34847772-34847791 MS.gene071036:CDS 40.0%
AACTATGGTGGTTGGAAAGC+TGG + chr7.3:34848106-34848125 MS.gene071036:CDS 45.0%
AATCCAAGCGGAAACTATGG+TGG + chr7.3:34848094-34848113 MS.gene071036:CDS 45.0%
CAAAGGAGTAACGTTGTGCT+TGG - chr7.3:34847922-34847941 None:intergenic 45.0%
CAAGGATACACACAATTCGG+TGG + chr7.3:34847998-34848017 MS.gene071036:CDS 45.0%
CTCTTTCCTATTTGCAGGCT+GGG + chr7.3:34848039-34848058 MS.gene071036:CDS 45.0%
GATTGAACCAACTGCTCAAC+AGG - chr7.3:34847962-34847981 None:intergenic 45.0%
GCAGGCTGGGACAAAAATTT+CGG + chr7.3:34848052-34848071 MS.gene071036:CDS 45.0%
GGAAAGAGACTCCAAATGGT+CGG - chr7.3:34848027-34848046 None:intergenic 45.0%
GGAAATGCTGGTACTGCAAT+AGG + chr7.3:34847728-34847747 MS.gene071036:CDS 45.0%
GGAGTCTCTTTCCTATTTGC+AGG + chr7.3:34848034-34848053 MS.gene071036:CDS 45.0%
GTACCAAGTGGAGTATGCAA+TGG + chr7.3:34847697-34847716 MS.gene071036:CDS 45.0%
TCTCTTTCCTATTTGCAGGC+TGG + chr7.3:34848038-34848057 MS.gene071036:CDS 45.0%
TTAGAATTGACTGTGCTGCC+TGG - chr7.3:34848150-34848169 None:intergenic 45.0%
! AGTTGAGGTTTGAAGCAGCT+TGG - chr7.3:34847804-34847823 None:intergenic 45.0%
! TTTTGTCCCAGCCTGCAAAT+AGG - chr7.3:34848048-34848067 None:intergenic 45.0%
!! CATTGGCTCTTGGTAAGCAA+AGG - chr7.3:34847939-34847958 None:intergenic 45.0%
AAAGACGGGGTTGTTCTGGT+TGG + chr7.3:34847761-34847780 MS.gene071036:CDS 50.0%
ACCAACTGCTCAACAGGCAT+TGG - chr7.3:34847956-34847975 None:intergenic 50.0%
ACTTGGTACAGACGTCCTTC+AGG - chr7.3:34847686-34847705 None:intergenic 50.0%
ATGCAGTGTCTGTTGCTGGT+TGG - chr7.3:34848375-34848394 None:intergenic 50.0%
ATGTAGCATGTGCTGTCGCT+GGG + chr7.3:34847855-34847874 MS.gene071036:CDS 50.0%
CAACCACCATAGTTTCCGCT+TGG - chr7.3:34848100-34848119 None:intergenic 50.0%
CAAGCGGAAACTATGGTGGT+TGG + chr7.3:34848098-34848117 MS.gene071036:CDS 50.0%
CCAAGTGGAGTATGCAATGG+AGG + chr7.3:34847700-34847719 MS.gene071036:CDS 50.0%
CCTCCATTGCATACTCCACT+TGG - chr7.3:34847703-34847722 None:intergenic 50.0%
GAGTATGCAATGGAGGCCAT+CGG + chr7.3:34847707-34847726 MS.gene071036:CDS 50.0%
GTCAAAAGACGGGGTTGTTC+TGG + chr7.3:34847757-34847776 MS.gene071036:CDS 50.0%
TGAAGGACGTCTGTACCAAG+TGG + chr7.3:34847685-34847704 MS.gene071036:CDS 50.0%
TGCAGTACCAGCATTTCCGA+TGG - chr7.3:34847726-34847745 None:intergenic 50.0%
! TGCAGTGTCTGTTGCTGGTT+GGG - chr7.3:34848374-34848393 None:intergenic 50.0%
!! GGTTGGAAAGCTGGTGCTAT+TGG + chr7.3:34848115-34848134 MS.gene071036:CDS 50.0%
!! TGCTATTGGTGCCAACAACC+AGG + chr7.3:34848129-34848148 MS.gene071036:CDS 50.0%
ATGGAGGCCATCGGAAATGC+TGG + chr7.3:34847716-34847735 MS.gene071036:CDS 55.0%
CATGTAGCATGTGCTGTCGC+TGG + chr7.3:34847854-34847873 MS.gene071036:CDS 55.0%
GCCAATGCCTGTTGAGCAGT+TGG + chr7.3:34847952-34847971 MS.gene071036:CDS 55.0%
TGACTGTGCTGCCTGGTTGT+TGG - chr7.3:34848143-34848162 None:intergenic 55.0%
TTCGGTGGTCTCCGACCATT+TGG + chr7.3:34848013-34848032 MS.gene071036:CDS 55.0%
!! GCTCAACAGGCATTGGCTCT+TGG - chr7.3:34847949-34847968 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 34847644 34848396 34847644 ID=MS.gene071036
chr7.3 mRNA 34847644 34848396 34847644 ID=MS.gene071036.t1;Parent=MS.gene071036
chr7.3 exon 34847644 34848396 34847644 ID=MS.gene071036.t1.exon1;Parent=MS.gene071036.t1
chr7.3 CDS 34847644 34848396 34847644 ID=cds.MS.gene071036.t1;Parent=MS.gene071036.t1
Gene Sequence

>MS.gene071036

ATGTCTCGAAGATATGATAGCCGTACAACAATATTCTCTCCTGAAGGACGTCTGTACCAAGTGGAGTATGCAATGGAGGCCATCGGAAATGCTGGTACTGCAATAGGAATCTTGTCAAAAGACGGGGTTGTTCTGGTTGGTGAAAAGAAGGTGACATCCAAGCTGCTTCAAACCTCAACTTCAACTGAGAAAATGTACAAGATCGATGATCATGTAGCATGTGCTGTCGCTGGGATTATGTCTGACGCCAACATCCTAATCAATACTGCTAGGATCCAAGCACAACGTTACTCCTTTGCTTACCAAGAGCCAATGCCTGTTGAGCAGTTGGTTCAATCTCTCTGTGATACTAAGCAAGGATACACACAATTCGGTGGTCTCCGACCATTTGGAGTCTCTTTCCTATTTGCAGGCTGGGACAAAAATTTCGGCTTTCAACTTTACATGAGTAATCCAAGCGGAAACTATGGTGGTTGGAAAGCTGGTGCTATTGGTGCCAACAACCAGGCAGCACAGTCAATTCTAAAACAGGACTACAAGGATGATATCACAAGGGAAGAAGCAGTTCAACTTGCATTGAAGGTTCTGAGTAAAACTATGGACAGCACTAGTCTTACCTCAGAAAAGCTTGAACTTGCAGAAGTTTTTCTTTCACCCTCTGGAAAAGTCAAGTATCAAGTTTGCTCCTCAGAGAATTTGACTAAGCTATTGGTGAAGTCTGGGGTGACCCAACCAGCAACAGACACTGCATAG

Protein sequence

>MS.gene071036.t1

MSRRYDSRTTIFSPEGRLYQVEYAMEAIGNAGTAIGILSKDGVVLVGEKKVTSKLLQTSTSTEKMYKIDDHVACAVAGIMSDANILINTARIQAQRYSFAYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNFGFQLYMSNPSGNYGGWKAGAIGANNQAAQSILKQDYKDDITREEAVQLALKVLSKTMDSTSLTSEKLELAEVFLSPSGKVKYQVCSSENLTKLLVKSGVTQPATDTA