Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071076.t1 | XP_003614194.2 | 98.6 | 74 | 1 | 0 | 1 | 74 | 279 | 352 | 1.60E-31 | 144.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071076.t1 | G7JWX2 | 98.6 | 74 | 1 | 0 | 1 | 74 | 339 | 412 | 1.1e-31 | 144.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene071076 | 0.803286 | 3.75E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene071076 | 0.803109 | 4.08E-49 | -1.69E-46 |
| MS.gene049283 | MS.gene071076 | 0.825953 | 3.54E-54 | -1.69E-46 |
| MS.gene049653 | MS.gene071076 | 0.806329 | 8.67E-50 | -1.69E-46 |
| MS.gene049821 | MS.gene071076 | 0.801195 | 1.01E-48 | -1.69E-46 |
| MS.gene049983 | MS.gene071076 | 0.823806 | 1.14E-53 | -1.69E-46 |
| MS.gene050437 | MS.gene071076 | 0.804791 | 1.82E-49 | -1.69E-46 |
| MS.gene050646 | MS.gene071076 | 0.813065 | 3.09E-51 | -1.69E-46 |
| MS.gene050654 | MS.gene071076 | 0.812088 | 5.05E-51 | -1.69E-46 |
| MS.gene050689 | MS.gene071076 | 0.819156 | 1.35E-52 | -1.69E-46 |
| MS.gene050752 | MS.gene071076 | 0.822634 | 2.13E-53 | -1.69E-46 |
| MS.gene050955 | MS.gene071076 | 0.827462 | 1.54E-54 | -1.69E-46 |
| MS.gene051003 | MS.gene071076 | 0.829357 | 5.39E-55 | -1.69E-46 |
| MS.gene051243 | MS.gene071076 | 0.807466 | 4.99E-50 | -1.69E-46 |
| MS.gene051343 | MS.gene071076 | 0.837379 | 5.38E-57 | -1.69E-46 |
| MS.gene051363 | MS.gene071076 | 0.826515 | 2.60E-54 | -1.69E-46 |
| MS.gene051365 | MS.gene071076 | 0.815035 | 1.14E-51 | -1.69E-46 |
| MS.gene051597 | MS.gene071076 | 0.816964 | 4.21E-52 | -1.69E-46 |
| MS.gene051856 | MS.gene071076 | 0.824698 | 7.01E-54 | -1.69E-46 |
| MS.gene051975 | MS.gene071076 | 0.814274 | 1.68E-51 | -1.69E-46 |
| MS.gene052072 | MS.gene071076 | 0.802514 | 5.41E-49 | -1.69E-46 |
| MS.gene052108 | MS.gene071076 | 0.807416 | 5.11E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene071076 | 0.803209 | 3.89E-49 | -1.69E-46 |
| MS.gene052954 | MS.gene071076 | 0.827801 | 1.28E-54 | -1.69E-46 |
| MS.gene053008 | MS.gene071076 | 0.806491 | 8.02E-50 | -1.69E-46 |
| MS.gene053058 | MS.gene071076 | 0.816867 | 4.43E-52 | -1.69E-46 |
| MS.gene05364 | MS.gene071076 | 0.82639 | 2.78E-54 | -1.69E-46 |
| MS.gene053931 | MS.gene071076 | 0.832999 | 6.86E-56 | -1.69E-46 |
| MS.gene054403 | MS.gene071076 | 0.821437 | 4.04E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene071076 | 0.810022 | 1.42E-50 | -1.69E-46 |
| MS.gene054657 | MS.gene071076 | 0.800463 | 1.42E-48 | -1.69E-46 |
| MS.gene05517 | MS.gene071076 | 0.826146 | 3.18E-54 | -1.69E-46 |
| MS.gene055513 | MS.gene071076 | 0.807131 | 5.87E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene071076 | 0.835417 | 1.70E-56 | -1.69E-46 |
| MS.gene055841 | MS.gene071076 | 0.8338 | 4.33E-56 | -1.69E-46 |
| MS.gene056480 | MS.gene071076 | 0.819414 | 1.18E-52 | -1.69E-46 |
| MS.gene056699 | MS.gene071076 | 0.829302 | 5.55E-55 | -1.69E-46 |
| MS.gene056776 | MS.gene071076 | 0.816366 | 5.74E-52 | -1.69E-46 |
| MS.gene056787 | MS.gene071076 | 0.812441 | 4.23E-51 | -1.69E-46 |
| MS.gene057034 | MS.gene071076 | 0.841504 | 4.57E-58 | -1.69E-46 |
| MS.gene057102 | MS.gene071076 | 0.80521 | 1.49E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene071076 | 0.86658 | 2.57E-65 | -1.69E-46 |
| MS.gene057371 | MS.gene071076 | 0.830307 | 3.16E-55 | -1.69E-46 |
| MS.gene057392 | MS.gene071076 | 0.835258 | 1.86E-56 | -1.69E-46 |
| MS.gene057693 | MS.gene071076 | 0.80005 | 1.73E-48 | -1.69E-46 |
| MS.gene057694 | MS.gene071076 | 0.808992 | 2.36E-50 | -1.69E-46 |
| MS.gene057705 | MS.gene071076 | 0.805958 | 1.04E-49 | -1.69E-46 |
| MS.gene057855 | MS.gene071076 | 0.806823 | 6.82E-50 | -1.69E-46 |
| MS.gene058008 | MS.gene071076 | 0.83101 | 2.13E-55 | -1.69E-46 |
| MS.gene058009 | MS.gene071076 | 0.820758 | 5.79E-53 | -1.69E-46 |
| MS.gene058126 | MS.gene071076 | 0.806533 | 7.85E-50 | -1.69E-46 |
| MS.gene058292 | MS.gene071076 | 0.801719 | 7.89E-49 | -1.69E-46 |
| MS.gene058781 | MS.gene071076 | 0.808928 | 2.43E-50 | -1.69E-46 |
| MS.gene058783 | MS.gene071076 | 0.828338 | 9.51E-55 | -1.69E-46 |
| MS.gene058976 | MS.gene071076 | 0.825749 | 3.95E-54 | -1.69E-46 |
| MS.gene059146 | MS.gene071076 | 0.814392 | 1.58E-51 | -1.69E-46 |
| MS.gene05985 | MS.gene071076 | -0.809419 | 1.91E-50 | -1.69E-46 |
| MS.gene059965 | MS.gene071076 | 0.805445 | 1.33E-49 | -1.69E-46 |
| MS.gene060244 | MS.gene071076 | 0.877198 | 7.62E-69 | -1.69E-46 |
| MS.gene060365 | MS.gene071076 | 0.848198 | 7.14E-60 | -1.69E-46 |
| MS.gene060935 | MS.gene071076 | 0.820361 | 7.15E-53 | -1.69E-46 |
| MS.gene060937 | MS.gene071076 | 0.815728 | 7.97E-52 | -1.69E-46 |
| MS.gene061100 | MS.gene071076 | 0.803186 | 3.93E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071076.t1 | MTR_5g046110 | 98.649 | 74 | 1 | 0 | 1 | 74 | 339 | 412 | 1.04e-45 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071076.t1 | AT4G36470 | 59.211 | 76 | 28 | 1 | 2 | 74 | 286 | 361 | 5.32e-23 | 89.7 |
Find 19 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACTTTGGAGAAAAGATTT+TGG | 0.182691 | 5.1:+34131344 | MS.gene071076:CDS |
| GAAAGCAGGGTCATTGAAAT+TGG | 0.298092 | 5.1:+34131215 | MS.gene071076:CDS |
| GGTTAGAAATCATTGATTCT+TGG | 0.390675 | 5.1:-34131323 | None:intergenic |
| GTCATTGAAATTGGAGAGGT+TGG | 0.423951 | 5.1:+34131224 | MS.gene071076:CDS |
| TAGATAAGAAGGAGCAAGGC+AGG | 0.435823 | 5.1:+34131259 | MS.gene071076:CDS |
| TCAATGATTTCTAACCACTT+TGG | 0.458721 | 5.1:+34131330 | MS.gene071076:CDS |
| GTTTGAGAACTATGCAATGC+TGG | 0.485835 | 5.1:+34131374 | MS.gene071076:CDS |
| GAAGATGAAGTAATGAAAGC+AGG | 0.486603 | 5.1:+34131201 | None:intergenic |
| AATGTTTGACATAGATAAGA+AGG | 0.500046 | 5.1:+34131248 | MS.gene071076:CDS |
| AGAAATCATTGATTCTTGGA+TGG | 0.518959 | 5.1:-34131319 | None:intergenic |
| TGATGAAGAAATTGCAAAGG+AGG | 0.543048 | 5.1:+34131398 | MS.gene071076:CDS |
| GAGCAAGGCAGGGAGAGCTA+TGG | 0.556285 | 5.1:+34131270 | MS.gene071076:CDS |
| GACATAGATAAGAAGGAGCA+AGG | 0.574472 | 5.1:+34131255 | MS.gene071076:CDS |
| AGAACAAGAACAAACGTGAT+GGG | 0.584403 | 5.1:-34131429 | None:intergenic |
| AAGATGAAGTAATGAAAGCA+GGG | 0.599911 | 5.1:+34131202 | None:intergenic |
| CTATGGTACAGATGTTGCAA+AGG | 0.624437 | 5.1:+34131287 | MS.gene071076:CDS |
| AGATAAGAAGGAGCAAGGCA+GGG | 0.631315 | 5.1:+34131260 | MS.gene071076:CDS |
| CAGGGTCATTGAAATTGGAG+AGG | 0.633479 | 5.1:+34131220 | MS.gene071076:CDS |
| GGTTGATGAAGAAATTGCAA+AGG | 0.715601 | 5.1:+34131395 | MS.gene071076:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATGTTTGACATAGATAAGA+AGG | + | chr5.1:34131248-34131267 | MS.gene071076:CDS | 25.0% |
| TCAATGATTTCTAACCACTT+TGG | + | chr5.1:34131330-34131349 | MS.gene071076:CDS | 30.0% | |
| !! | AGAAATCATTGATTCTTGGA+TGG | - | chr5.1:34131322-34131341 | None:intergenic | 30.0% |
| !! | GGTTAGAAATCATTGATTCT+TGG | - | chr5.1:34131326-34131345 | None:intergenic | 30.0% |
| TGATGAAGAAATTGCAAAGG+AGG | + | chr5.1:34131398-34131417 | MS.gene071076:CDS | 35.0% | |
| ! | CCAAAATCTTTTCTCCAAAG+TGG | - | chr5.1:34131347-34131366 | None:intergenic | 35.0% |
| !! | CCACTTTGGAGAAAAGATTT+TGG | + | chr5.1:34131344-34131363 | MS.gene071076:CDS | 35.0% |
| !! | GGTTGATGAAGAAATTGCAA+AGG | + | chr5.1:34131395-34131414 | MS.gene071076:CDS | 35.0% |
| CTATGGTACAGATGTTGCAA+AGG | + | chr5.1:34131287-34131306 | MS.gene071076:CDS | 40.0% | |
| GAAAGCAGGGTCATTGAAAT+TGG | + | chr5.1:34131215-34131234 | MS.gene071076:CDS | 40.0% | |
| GACATAGATAAGAAGGAGCA+AGG | + | chr5.1:34131255-34131274 | MS.gene071076:CDS | 40.0% | |
| GTCATTGAAATTGGAGAGGT+TGG | + | chr5.1:34131224-34131243 | MS.gene071076:CDS | 40.0% | |
| GTTTGAGAACTATGCAATGC+TGG | + | chr5.1:34131374-34131393 | MS.gene071076:CDS | 40.0% | |
| AGATAAGAAGGAGCAAGGCA+GGG | + | chr5.1:34131260-34131279 | MS.gene071076:CDS | 45.0% | |
| CAGGGTCATTGAAATTGGAG+AGG | + | chr5.1:34131220-34131239 | MS.gene071076:CDS | 45.0% | |
| TAGATAAGAAGGAGCAAGGC+AGG | + | chr5.1:34131259-34131278 | MS.gene071076:CDS | 45.0% | |
| GAGCAAGGCAGGGAGAGCTA+TGG | + | chr5.1:34131270-34131289 | MS.gene071076:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 34131213 | 34131436 | 34131213 | ID=MS.gene071076 |
| chr5.1 | mRNA | 34131213 | 34131436 | 34131213 | ID=MS.gene071076.t1;Parent=MS.gene071076 |
| chr5.1 | exon | 34131213 | 34131436 | 34131213 | ID=MS.gene071076.t1.exon1;Parent=MS.gene071076.t1 |
| chr5.1 | CDS | 34131213 | 34131436 | 34131213 | ID=cds.MS.gene071076.t1;Parent=MS.gene071076.t1 |
| Gene Sequence |
| Protein sequence |
