AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23491


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23491.t1 MTR_7g096840 97.765 179 4 0 1 179 1 179 6.43e-125 349
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23491.t1 AT2G44820 58.268 127 50 2 24 149 20 144 2.60e-36 125
MS.gene23491.t1 AT2G44820 58.268 127 50 2 24 149 39 163 3.11e-36 125

Find 33 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTATGATTCTAGGACGTTT+TGG 0.217863 7.3:+14532234 MS.gene23491:CDS
ATTGGTCGTGCCACACTTAA+AGG 0.224539 7.3:-14532078 None:intergenic
TGCTTATTGTTATGTTCTCT+TGG 0.258679 7.3:-14531484 None:intergenic
TAGAGAAAATACTGAAGTTT+TGG 0.269693 7.3:-14531546 None:intergenic
CAACTGGAAACATGATAAAA+AGG 0.272902 7.3:+14532446 MS.gene23491:CDS
GCCTTTCAGCGTATGATTCT+AGG 0.290954 7.3:+14532225 MS.gene23491:intron
AATGGACATCAGGAAGATTA+TGG 0.351249 7.3:+14531516 MS.gene23491:CDS
CCAGTGTCATGACCTCTTGT+GGG 0.400870 7.3:-14532387 None:intergenic
AGGAAAATGAAATGGACATC+AGG 0.425149 7.3:+14531506 MS.gene23491:CDS
TTATTGTTATGTTCTCTTGG+AGG 0.434023 7.3:-14531481 None:intergenic
AACTTCAAACCTCTGTCCTC+TGG 0.439918 7.3:-14532306 None:intergenic
GACATGGAAAGAGCGCAAAA+AGG 0.445441 7.3:+14531888 MS.gene23491:CDS
CAATAAGCAGGAAAATGAAA+TGG 0.447339 7.3:+14531498 MS.gene23491:CDS
GGTTGTTTCCCTTGGTGGGA+AGG 0.454986 7.3:+14531924 MS.gene23491:CDS
GATAGGAAGGTTGTTTCCCT+TGG 0.461391 7.3:+14531916 MS.gene23491:CDS
TCCAGTGTCATGACCTCTTG+TGG 0.466337 7.3:-14532388 None:intergenic
AGGTAATCTTTGATTCTTGA+GGG 0.502643 7.3:-14532058 None:intergenic
AAGAAACAAGCTCACGGACG+TGG 0.521086 7.3:+14531457 MS.gene23491:CDS
AAGGTAATCTTTGATTCTTG+AGG 0.525330 7.3:-14532059 None:intergenic
GGAAGGTTGTTTCCCTTGGT+GGG 0.530525 7.3:+14531920 MS.gene23491:CDS
AAGAGAACATAACAATAAGC+AGG 0.534086 7.3:+14531486 MS.gene23491:CDS
AGGAAGGTTGTTTCCCTTGG+TGG 0.540449 7.3:+14531919 MS.gene23491:CDS
TTGAAGTCAACACCCACAAG+AGG 0.593893 7.3:+14532375 MS.gene23491:CDS
TCAAAGATTACCTTTAAGTG+TGG 0.597312 7.3:+14532068 MS.gene23491:CDS
GGTAATCTTTGATTCTTGAG+GGG 0.603282 7.3:-14532057 None:intergenic
CCCACAAGAGGTCATGACAC+TGG 0.620094 7.3:+14532387 MS.gene23491:CDS
TAGGCAACTCGCATATGACA+TGG 0.625414 7.3:+14531872 MS.gene23491:intron
AGACCTCACCTTCCCACCAA+GGG 0.627792 7.3:-14531932 None:intergenic
AGAGGTTTGAAGTTAAGTGA+AGG 0.641153 7.3:+14532315 MS.gene23491:CDS
TACAAGAAGAAACAAGCTCA+CGG 0.651983 7.3:+14531451 MS.gene23491:CDS
TAGACCTCACCTTCCCACCA+AGG 0.660546 7.3:-14531933 None:intergenic
AGCTGGAAAACACAAACCAG+AGG 0.689560 7.3:+14532290 MS.gene23491:CDS
AAACACAAACCAGAGGACAG+AGG 0.717192 7.3:+14532297 MS.gene23491:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTCATTATTGTAAATTTG+GGG + chr7.3:14531764-14531783 MS.gene23491:intron 15.0%
!! CATTTCATTATTGTAAATTT+GGG + chr7.3:14531763-14531782 MS.gene23491:intron 15.0%
!! TCATTTCATTATTGTAAATT+TGG + chr7.3:14531762-14531781 MS.gene23491:intron 15.0%
!! AGTAGTCATTATAAAAAAGA+AGG - chr7.3:14531837-14531856 None:intergenic 20.0%
!! TAAAACATCCAATTCAAATT+CGG - chr7.3:14531720-14531739 None:intergenic 20.0%
!! TGTTGGAGTTAAATAATTTA+TGG + chr7.3:14531787-14531806 MS.gene23491:intron 20.0%
! AACTGGAAACATGATAAAAA+GGG + chr7.3:14532447-14532466 MS.gene23491:CDS 25.0%
! AGTTGTACAAACAAATAAAG+AGG - chr7.3:14532167-14532186 None:intergenic 25.0%
! GGAAAAAATATGTCAAATAC+AGG + chr7.3:14532408-14532427 MS.gene23491:CDS 25.0%
! TACTCATTGCAATATATCTT+AGG + chr7.3:14531853-14531872 MS.gene23491:intron 25.0%
! TATCATGTTCTATATGCAAA+TGG + chr7.3:14531978-14531997 MS.gene23491:intron 25.0%
! TCAAATACAGGAAAAAGAAA+AGG + chr7.3:14532420-14532439 MS.gene23491:CDS 25.0%
! TGATAGAATTAATTACCTCT+TGG - chr7.3:14532140-14532159 None:intergenic 25.0%
!! TAGAGAAAATACTGAAGTTT+TGG - chr7.3:14531549-14531568 None:intergenic 25.0%
!!! TGTTTTTGTTTCTTCATCAT+TGG - chr7.3:14532099-14532118 None:intergenic 25.0%
AAAAAAGAAGGTAACAAACC+AGG - chr7.3:14531825-14531844 None:intergenic 30.0%
AAGAGAACATAACAATAAGC+AGG + chr7.3:14531486-14531505 MS.gene23491:CDS 30.0%
AAGGTAATCTTTGATTCTTG+AGG - chr7.3:14532062-14532081 None:intergenic 30.0%
AATACAGGAAAAAGAAAAGG+AGG + chr7.3:14532423-14532442 MS.gene23491:CDS 30.0%
AATGGTATTATCACAATGCT+CGG + chr7.3:14531996-14532015 MS.gene23491:intron 30.0%
AGGTAATCTTTGATTCTTGA+GGG - chr7.3:14532061-14532080 None:intergenic 30.0%
CAACTGGAAACATGATAAAA+AGG + chr7.3:14532446-14532465 MS.gene23491:CDS 30.0%
CAATAAGCAGGAAAATGAAA+TGG + chr7.3:14531498-14531517 MS.gene23491:CDS 30.0%
CGTCATTGATCATATATTTG+AGG - chr7.3:14531653-14531672 None:intergenic 30.0%
GATGAAGAAACAAAAACAGA+GGG + chr7.3:14532101-14532120 MS.gene23491:CDS 30.0%
TCAAAGATTACCTTTAAGTG+TGG + chr7.3:14532068-14532087 MS.gene23491:CDS 30.0%
TGATAAGACCGAATTTGAAT+TGG + chr7.3:14531709-14531728 MS.gene23491:intron 30.0%
TGATGAAGAAACAAAAACAG+AGG + chr7.3:14532100-14532119 MS.gene23491:CDS 30.0%
TGCTTATTGTTATGTTCTCT+TGG - chr7.3:14531487-14531506 None:intergenic 30.0%
TGTACAAACAAATAAAGAGG+AGG - chr7.3:14532164-14532183 None:intergenic 30.0%
TTATTGTTATGTTCTCTTGG+AGG - chr7.3:14531484-14531503 None:intergenic 30.0%
! AGTGAAGGTCGATTTAAAAA+TGG + chr7.3:14532330-14532349 MS.gene23491:CDS 30.0%
! TTATTGTAAATTTGGGGTGT+TGG + chr7.3:14531770-14531789 MS.gene23491:intron 30.0%
AAACATGATAAAAAGGGTGG+TGG + chr7.3:14532453-14532472 MS.gene23491:CDS 35.0%
AATGGACATCAGGAAGATTA+TGG + chr7.3:14531516-14531535 MS.gene23491:CDS 35.0%
AGGAAAATGAAATGGACATC+AGG + chr7.3:14531506-14531525 MS.gene23491:CDS 35.0%
CAAAAAGGTTGAAGATAGGA+AGG + chr7.3:14531903-14531922 MS.gene23491:CDS 35.0%
GAATAAGAGCAGATGAAACA+CGG - chr7.3:14532035-14532054 None:intergenic 35.0%
GCACAAGTGGTACATATATA+AGG - chr7.3:14532207-14532226 None:intergenic 35.0%
GGTAATCTTTGATTCTTGAG+GGG - chr7.3:14532060-14532079 None:intergenic 35.0%
TACAAGAAGAAACAAGCTCA+CGG + chr7.3:14531451-14531470 MS.gene23491:CDS 35.0%
TGGAAACATGATAAAAAGGG+TGG + chr7.3:14532450-14532469 MS.gene23491:CDS 35.0%
TTATGGTAAGCATATTCACC+TGG + chr7.3:14531804-14531823 MS.gene23491:intron 35.0%
! AGAGGTTTGAAGTTAAGTGA+AGG + chr7.3:14532315-14532334 MS.gene23491:CDS 35.0%
!!! GTATGATTCTAGGACGTTTT+GGG + chr7.3:14532235-14532254 MS.gene23491:CDS 35.0%
!!! TATGATTCTAGGACGTTTTG+GGG + chr7.3:14532236-14532255 MS.gene23491:CDS 35.0%
AGCGCAAAAAGGTTGAAGAT+AGG + chr7.3:14531899-14531918 MS.gene23491:CDS 40.0%
GAAAAAGAAAAGGAGGCAAC+TGG + chr7.3:14532430-14532449 MS.gene23491:CDS 40.0%
GAAGAAACAAAAACAGAGGG+AGG + chr7.3:14532104-14532123 MS.gene23491:CDS 40.0%
TCCTAGAATCATACGCTGAA+AGG - chr7.3:14532229-14532248 None:intergenic 40.0%
! TAAAAAGGGTGGTGGTAAGA+AGG + chr7.3:14532461-14532480 MS.gene23491:CDS 40.0%
!!! ATGATTCTAGGACGTTTTGG+GGG + chr7.3:14532237-14532256 MS.gene23491:CDS 40.0%
!!! CGTATGATTCTAGGACGTTT+TGG + chr7.3:14532234-14532253 MS.gene23491:CDS 40.0%
AAACACAAACCAGAGGACAG+AGG + chr7.3:14532297-14532316 MS.gene23491:CDS 45.0%
AACTTCAAACCTCTGTCCTC+TGG - chr7.3:14532309-14532328 None:intergenic 45.0%
AGCAGCAGTAAAAAACCAGC+TGG + chr7.3:14532273-14532292 MS.gene23491:CDS 45.0%
AGCTGGAAAACACAAACCAG+AGG + chr7.3:14532290-14532309 MS.gene23491:CDS 45.0%
ATTGGTCGTGCCACACTTAA+AGG - chr7.3:14532081-14532100 None:intergenic 45.0%
GCCTTTCAGCGTATGATTCT+AGG + chr7.3:14532225-14532244 MS.gene23491:intron 45.0%
GGAGGAAAAAATGCTCCAAG+AGG + chr7.3:14532122-14532141 MS.gene23491:CDS 45.0%
TAGGCAACTCGCATATGACA+TGG + chr7.3:14531872-14531891 MS.gene23491:intron 45.0%
TTGAAGTCAACACCCACAAG+AGG + chr7.3:14532375-14532394 MS.gene23491:CDS 45.0%
! GACATGGAAAGAGCGCAAAA+AGG + chr7.3:14531888-14531907 MS.gene23491:CDS 45.0%
! GATAGGAAGGTTGTTTCCCT+TGG + chr7.3:14531916-14531935 MS.gene23491:CDS 45.0%
!! TCTGGTTTGTGTTTTCCAGC+TGG - chr7.3:14532291-14532310 None:intergenic 45.0%
AAGAAACAAGCTCACGGACG+TGG + chr7.3:14531457-14531476 MS.gene23491:CDS 50.0%
CCAGTGTCATGACCTCTTGT+GGG - chr7.3:14532390-14532409 None:intergenic 50.0%
TCCAGTGTCATGACCTCTTG+TGG - chr7.3:14532391-14532410 None:intergenic 50.0%
! AGGAAGGTTGTTTCCCTTGG+TGG + chr7.3:14531919-14531938 MS.gene23491:CDS 50.0%
! CATACGCTGAAAGGCACAAG+TGG - chr7.3:14532220-14532239 None:intergenic 50.0%
! GGAAGGTTGTTTCCCTTGGT+GGG + chr7.3:14531920-14531939 MS.gene23491:CDS 50.0%
!!! TCTAGGACGTTTTGGGGGAA+AGG + chr7.3:14532242-14532261 MS.gene23491:CDS 50.0%
AGACCTCACCTTCCCACCAA+GGG - chr7.3:14531935-14531954 None:intergenic 55.0%
CCCACAAGAGGTCATGACAC+TGG + chr7.3:14532387-14532406 MS.gene23491:CDS 55.0%
TAGACCTCACCTTCCCACCA+AGG - chr7.3:14531936-14531955 None:intergenic 55.0%
TTTCCCTTGGTGGGAAGGTG+AGG + chr7.3:14531929-14531948 MS.gene23491:intron 55.0%
! GGTTGTTTCCCTTGGTGGGA+AGG + chr7.3:14531924-14531943 MS.gene23491:CDS 55.0%
!!! CGTTTTGGGGGAAAGGATGG+TGG + chr7.3:14532249-14532268 MS.gene23491:CDS 55.0%
!!! GGACGTTTTGGGGGAAAGGA+TGG + chr7.3:14532246-14532265 MS.gene23491:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 14531439 14532497 14531439 ID=MS.gene23491
chr7.3 mRNA 14531439 14532497 14531439 ID=MS.gene23491.t1;Parent=MS.gene23491
chr7.3 exon 14531439 14531556 14531439 ID=MS.gene23491.t1.exon1;Parent=MS.gene23491.t1
chr7.3 CDS 14531439 14531556 14531439 ID=cds.MS.gene23491.t1;Parent=MS.gene23491.t1
chr7.3 exon 14531875 14531945 14531875 ID=MS.gene23491.t1.exon2;Parent=MS.gene23491.t1
chr7.3 CDS 14531875 14531945 14531875 ID=cds.MS.gene23491.t1;Parent=MS.gene23491.t1
chr7.3 exon 14532057 14532143 14532057 ID=MS.gene23491.t1.exon3;Parent=MS.gene23491.t1
chr7.3 CDS 14532057 14532143 14532057 ID=cds.MS.gene23491.t1;Parent=MS.gene23491.t1
chr7.3 exon 14532234 14532497 14532234 ID=MS.gene23491.t1.exon4;Parent=MS.gene23491.t1
chr7.3 CDS 14532234 14532497 14532234 ID=cds.MS.gene23491.t1;Parent=MS.gene23491.t1
Gene Sequence

>MS.gene23491

ATGGGAAAGATGTACAAGAAGAAACAAGCTCACGGACGTGGACCTCCAAGAGAACATAACAATAAGCAGGAAAATGAAATGGACATCAGGAAGATTATGGAAGACGTCCAAAACTTCAGTATTTTCTCTACTTTTTTTTTTTTTTTTTTAAATTATTTCACATTGAATGTTGTTTATTTTCTCATTGAATCTGTTTTCTGTTTTTGATGCACCTCAAATATATGATCAATGACGTTTCTGCGTTATGATTTTGCTTATGATATGTTTAAATGATAAGACCGAATTTGAATTGGATGTTTTAATGCTAAGAACAAAATATCTGTTCATTTCATTATTGTAAATTTGGGGTGTTGGAGTTAAATAATTTATGGTAAGCATATTCACCTGGTTTGTTACCTTCTTTTTTATAATGACTACTCATTGCAATATATCTTAGGCAACTCGCATATGACATGGAAAGAGCGCAAAAAGGTTGAAGATAGGAAGGTTGTTTCCCTTGGTGGGAAGGTGAGGTCTATTTATTGATGCTCTATTTTTTATATCATGTTCTATATGCAAATGGTATTATCACAATGCTCGGTGCACAAAATTCACCGTGTTTCATCTGCTCTTATTCAGCCCCTCAAGAATCAAAGATTACCTTTAAGTGTGGCACGACCAATGATGAAGAAACAAAAACAGAGGGAGGAAAAAATGCTCCAAGAGGTAATTAATTCTATCATCCTCCTCTTTATTTGTTTGTACAACTCTTACTTTCTTTTTATTCCTTATATATGTACCACTTGTGCCTTTCAGCGTATGATTCTAGGACGTTTTGGGGGAAAGGATGGTGGTAGCAGCAGTAAAAAACCAGCTGGAAAACACAAACCAGAGGACAGAGGTTTGAAGTTAAGTGAAGGTCGATTTAAAAATGGCATACTCGACGTGAAGCATTTGTTGAAGTCAACACCCACAAGAGGTCATGACACTGGAAAAAATATGTCAAATACAGGAAAAAGAAAAGGAGGCAACTGGAAACATGATAAAAAGGGTGGTGGTAAGAAGGATCAAATGTTTTAG

Protein sequence

>MS.gene23491.t1

MGKMYKKKQAHGRGPPREHNNKQENEMDIRKIMEDVQNFSNSHMTWKERKKVEDRKVVSLGGKPLKNQRLPLSVARPMMKKQKQREEKMLQERMILGRFGGKDGGSSSKKPAGKHKPEDRGLKLSEGRFKNGILDVKHLLKSTPTRGHDTGKNMSNTGKRKGGNWKHDKKGGGKKDQMF