AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28231


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28231.t1 MTR_3g026020 95.541 157 2 1 1 157 38 189 1.67e-99 284
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28231.t1 AT5G08050 64.103 156 41 2 1 155 15 156 2.05e-50 159

Find 36 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGTCGAGGTTGGATTAC+AGG 0.269782 3.3:+17839533 MS.gene28231:CDS
GAGACAGAAGGAACTGGTTT+GGG 0.333662 3.3:-17838012 None:intergenic
TGTGTTGTTGGTGGTTCTGC+TGG 0.362863 3.3:+17839573 MS.gene28231:CDS
TAAAACTGTAGCTTCTAAAA+AGG 0.383526 3.3:+17838035 MS.gene28231:CDS
AGAGACAGAAGGAACTGGTT+TGG 0.399810 3.3:-17838013 None:intergenic
TAGCGATAATCCTGCTTTCT+CGG 0.404741 3.3:-17839355 None:intergenic
CATTCTTGTGAGGAGTTTAA+CGG 0.405852 3.3:-17839280 None:intergenic
GTTGCTTTGATCTGTGTTGT+TGG 0.414772 3.3:+17839561 MS.gene28231:CDS
TGATAAAACACCAAGTTCTT+CGG 0.420793 3.3:-17839412 None:intergenic
TAAACCTGCTTTCTCTGCCT+TGG 0.422974 3.3:-17839322 None:intergenic
CTCTCAAAAGCCGAAGAACT+TGG 0.423172 3.3:+17839402 MS.gene28231:CDS
AACCTCGACAACATTATCTT+CGG 0.427394 3.3:-17839523 None:intergenic
CGTTAAACTCCTCACAAGAA+TGG 0.467112 3.3:+17839281 MS.gene28231:CDS
AAGAGTGATCCTGGAGTTGA+TGG 0.471176 3.3:-17839447 None:intergenic
CTATCAACAATAGAGAAACT+TGG 0.493062 3.3:+17839375 MS.gene28231:CDS
CTGAGTGTGAAGAGTGATCC+TGG 0.494258 3.3:-17839456 None:intergenic
ACCTCGACAACATTATCTTC+GGG 0.509562 3.3:-17839522 None:intergenic
GAAGATAATGTTGTCGAGGT+TGG 0.509847 3.3:+17839525 MS.gene28231:CDS
AACTGGTTTGGGAGTAGCCA+TGG 0.510271 3.3:-17838001 None:intergenic
TTATCAGCCGCCGAGAAAGC+AGG 0.520670 3.3:+17839345 MS.gene28231:CDS
GCTTTGATCTGTGTTGTTGG+TGG 0.528999 3.3:+17839564 MS.gene28231:CDS
CGAGGTTGGATTACAGGCTG+TGG 0.533900 3.3:+17839539 MS.gene28231:CDS
AATTCAACTGTATTCCCACT+TGG 0.537612 3.3:+17839222 MS.gene28231:CDS
TCCTAGGTTGTTCCCCAAGT+GGG 0.538069 3.3:-17839236 None:intergenic
TTAACCAAGGCAGAGAAAGC+AGG 0.541705 3.3:+17839318 MS.gene28231:CDS
GCTACTGATCCATCAACTCC+AGG 0.551246 3.3:+17839438 MS.gene28231:CDS
ATTCAACTGTATTCCCACTT+GGG 0.553323 3.3:+17839223 MS.gene28231:CDS
GTCGATGTTACAGCACTCCT+AGG 0.557906 3.3:-17839252 None:intergenic
TCAGTTGTTCCATTCTTGTG+AGG 0.560445 3.3:-17839290 None:intergenic
TCCCACTTGGGGAACAACCT+AGG 0.596244 3.3:+17839235 MS.gene28231:CDS
TTCAACTGTATTCCCACTTG+GGG 0.603934 3.3:+17839224 MS.gene28231:CDS
TCCCGAAGATAATGTTGTCG+AGG 0.604575 3.3:+17839521 MS.gene28231:CDS
GGAACAAGATAAACAAACAA+AGG 0.614999 3.3:-17839501 None:intergenic
CTCCTAGGTTGTTCCCCAAG+TGG 0.623775 3.3:-17839237 None:intergenic
CGATAATCCTGCTTTCTCGG+CGG 0.695629 3.3:-17839352 None:intergenic
ACAACTGAGACTGTTAACCA+AGG 0.730927 3.3:+17839305 MS.gene28231:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACAAGTAAGAATTATA+TGG - chr3.3:17838078-17838097 None:intergenic 15.0%
!! ATAAAGTAAAGTTTCTATGT+AGG - chr3.3:17838601-17838620 None:intergenic 20.0%
!! ATCCTTTCTCATAATTAAAT+TGG - chr3.3:17838188-17838207 None:intergenic 20.0%
!! TACCAATTTAATTATGAGAA+AGG + chr3.3:17838183-17838202 MS.gene28231:intron 20.0%
!!! ATAAACTGTAATTTGTCATA+GGG + chr3.3:17838327-17838346 MS.gene28231:intron 20.0%
!!! ATATTTGTTTTCCTTTGTTT+TGG + chr3.3:17838971-17838990 MS.gene28231:intron 20.0%
!!! TTATTTTATTTTCTGCAAGT+TGG - chr3.3:17839622-17839641 None:intergenic 20.0%
! AATTTACATAATGCCATGTT+TGG - chr3.3:17838759-17838778 None:intergenic 25.0%
! ATGGCATTATGTAAATTCTA+CGG + chr3.3:17838762-17838781 MS.gene28231:intron 25.0%
! ATTACAGTTTATGTATACCT+AGG - chr3.3:17838319-17838338 None:intergenic 25.0%
! ATTGAAAACGTAAAATAACC+AGG - chr3.3:17838564-17838583 None:intergenic 25.0%
! CATAGAAACTTTACTTTATC+AGG + chr3.3:17838602-17838621 MS.gene28231:intron 25.0%
! TATACTGTAAAAAAATCACC+TGG + chr3.3:17838543-17838562 MS.gene28231:intron 25.0%
!! CATAAACTGTAATTTGTCAT+AGG + chr3.3:17838326-17838345 MS.gene28231:intron 25.0%
!! TAAAACTGTAGCTTCTAAAA+AGG + chr3.3:17838035-17838054 MS.gene28231:CDS 25.0%
!! TAAACTGTAATTTGTCATAG+GGG + chr3.3:17838328-17838347 MS.gene28231:intron 25.0%
!! TGGAATCTTTAGGAAAAAAA+AGG + chr3.3:17838517-17838536 MS.gene28231:intron 25.0%
!! TTTAATGCTCTTTGATGATA+TGG + chr3.3:17838286-17838305 MS.gene28231:intron 25.0%
!!! AGCTTTGTTTTACTTCTTTT+AGG + chr3.3:17839477-17839496 MS.gene28231:CDS 25.0%
!!! TGGAAATGCTTTGTTTAAAT+AGG - chr3.3:17838739-17838758 None:intergenic 25.0%
ACCATATGAGTTGTACTTAT+CGG - chr3.3:17838402-17838421 None:intergenic 30.0%
AGTACAACTCATATGGTAAA+AGG + chr3.3:17838405-17838424 MS.gene28231:intron 30.0%
CCACTTTAATAAACGGTTTA+TGG - chr3.3:17839120-17839139 None:intergenic 30.0%
CCATAAACCGTTTATTAAAG+TGG + chr3.3:17839117-17839136 MS.gene28231:intron 30.0%
CTATCAACAATAGAGAAACT+TGG + chr3.3:17839375-17839394 MS.gene28231:CDS 30.0%
GGAACAAGATAAACAAACAA+AGG - chr3.3:17839504-17839523 None:intergenic 30.0%
GGTCTGATAAGTACTAAAAA+TGG + chr3.3:17838660-17838679 MS.gene28231:intron 30.0%
TGATAAAACACCAAGTTCTT+CGG - chr3.3:17839415-17839434 None:intergenic 30.0%
TGTTTGTTCTACCAAAACAA+AGG - chr3.3:17838985-17839004 None:intergenic 30.0%
TTATGCAATATTATGCTGTG+TGG + chr3.3:17838135-17838154 MS.gene28231:intron 30.0%
! AAACAAAGCATTTCCAAACA+TGG + chr3.3:17838743-17838762 MS.gene28231:intron 30.0%
! ACAGTCATTGTTATTGTCAT+AGG - chr3.3:17839028-17839047 None:intergenic 30.0%
! CTCTTTGATGATATGGTATA+TGG + chr3.3:17838293-17838312 MS.gene28231:intron 30.0%
!! ACTTTATCAGGCTTTTAAGT+AGG + chr3.3:17838614-17838633 MS.gene28231:intron 30.0%
!! ATGCTTTGTTTAAATAGGTC+TGG - chr3.3:17838734-17838753 None:intergenic 30.0%
!! TTTCTACGGTAATTTTCAAG+TGG + chr3.3:17839087-17839106 MS.gene28231:intron 30.0%
AACCTCGACAACATTATCTT+CGG - chr3.3:17839526-17839545 None:intergenic 35.0%
AATATTATGCTGTGTGGAAC+TGG + chr3.3:17838141-17838160 MS.gene28231:intron 35.0%
AATTCAACTGTATTCCCACT+TGG + chr3.3:17839222-17839241 MS.gene28231:CDS 35.0%
ACTAGTTCGAAAAGACATCT+GGG + chr3.3:17838919-17838938 MS.gene28231:intron 35.0%
ATTCAACTGTATTCCCACTT+GGG + chr3.3:17839223-17839242 MS.gene28231:CDS 35.0%
CATTCTTGTGAGGAGTTTAA+CGG - chr3.3:17839283-17839302 None:intergenic 35.0%
CGGTAGACCACTTTAATAAA+CGG - chr3.3:17839127-17839146 None:intergenic 35.0%
CTTTGTCTTAATGAGTGAGA+CGG + chr3.3:17838883-17838902 MS.gene28231:intron 35.0%
GCATTATGTAAATTCTACGG+TGG + chr3.3:17838765-17838784 MS.gene28231:intron 35.0%
GTACTATGAGTCGTAATTTC+AGG - chr3.3:17838835-17838854 None:intergenic 35.0%
TACTAGTTCGAAAAGACATC+TGG + chr3.3:17838918-17838937 MS.gene28231:intron 35.0%
TCCGATAAGTACAACTCATA+TGG + chr3.3:17838398-17838417 MS.gene28231:intron 35.0%
TCTCACTCATTAAGACAAAG+AGG - chr3.3:17838883-17838902 None:intergenic 35.0%
! AACTTTTGTCCCCAAAAAAC+AGG - chr3.3:17839159-17839178 None:intergenic 35.0%
! TACTTATCAGACCTTTTCGA+AGG - chr3.3:17838653-17838672 None:intergenic 35.0%
!!! TGTTTTTTGGGGACAAAAGT+TGG + chr3.3:17839158-17839177 MS.gene28231:intron 35.0%
AATACGTCAGACCTTCGAAA+AGG + chr3.3:17838639-17838658 MS.gene28231:intron 40.0%
ACAACTGAGACTGTTAACCA+AGG + chr3.3:17839305-17839324 MS.gene28231:CDS 40.0%
ACATTTGATATGCTGAGACG+CGG + chr3.3:17838436-17838455 MS.gene28231:intron 40.0%
ACCTCGACAACATTATCTTC+GGG - chr3.3:17839525-17839544 None:intergenic 40.0%
ACTCATATGGTAAAAGGCTG+AGG + chr3.3:17838411-17838430 MS.gene28231:intron 40.0%
CGTTAAACTCCTCACAAGAA+TGG + chr3.3:17839281-17839300 MS.gene28231:CDS 40.0%
CTCATATGGTAAAAGGCTGA+GGG + chr3.3:17838412-17838431 MS.gene28231:intron 40.0%
GAAGATAATGTTGTCGAGGT+TGG + chr3.3:17839525-17839544 MS.gene28231:CDS 40.0%
GATGATATGGTATATGGCCT+AGG + chr3.3:17838299-17838318 MS.gene28231:intron 40.0%
TAGCGATAATCCTGCTTTCT+CGG - chr3.3:17839358-17839377 None:intergenic 40.0%
TCAGTTGTTCCATTCTTGTG+AGG - chr3.3:17839293-17839312 None:intergenic 40.0%
TCATATGGTAAAAGGCTGAG+GGG + chr3.3:17838413-17838432 MS.gene28231:intron 40.0%
TTCAACTGTATTCCCACTTG+GGG + chr3.3:17839224-17839243 MS.gene28231:CDS 40.0%
TTCTACTTCCAAGAGTGACA+TGG + chr3.3:17839052-17839071 MS.gene28231:intron 40.0%
! GTTGCTTTGATCTGTGTTGT+TGG + chr3.3:17839561-17839580 MS.gene28231:CDS 40.0%
! GTTTTGTCGCTGGAATCTTT+AGG + chr3.3:17838507-17838526 MS.gene28231:intron 40.0%
! TTTCAAGTGTTAATCCACCG+TGG - chr3.3:17838808-17838827 None:intergenic 40.0%
!! CAAAAAACAGGCTGGTTCTA+CGG - chr3.3:17839147-17839166 None:intergenic 40.0%
!!! CTACGGTAATTTTCAAGTGG+TGG + chr3.3:17839090-17839109 MS.gene28231:intron 40.0%
!!! GCTACAGTTTTAGAGACAGA+AGG - chr3.3:17838027-17838046 None:intergenic 40.0%
!!! GTAGAACCAGCCTGTTTTTT+GGG + chr3.3:17839146-17839165 MS.gene28231:intron 40.0%
!!! GTTTTAGAGACAGAAGGAAC+TGG - chr3.3:17838021-17838040 None:intergenic 40.0%
!!! TAGAACCAGCCTGTTTTTTG+GGG + chr3.3:17839147-17839166 MS.gene28231:intron 40.0%
AAGAGTGACATGGTTGTCGT+CGG + chr3.3:17839062-17839081 MS.gene28231:intron 45.0%
AAGAGTGATCCTGGAGTTGA+TGG - chr3.3:17839450-17839469 None:intergenic 45.0%
AGAGACAGAAGGAACTGGTT+TGG - chr3.3:17838016-17838035 None:intergenic 45.0%
CATTTGATATGCTGAGACGC+GGG + chr3.3:17838437-17838456 MS.gene28231:intron 45.0%
CGACAAAACTCACACTCACA+CGG - chr3.3:17838496-17838515 None:intergenic 45.0%
CTCTCAAAAGCCGAAGAACT+TGG + chr3.3:17839402-17839421 MS.gene28231:CDS 45.0%
TAAACCTGCTTTCTCTGCCT+TGG - chr3.3:17839325-17839344 None:intergenic 45.0%
TCCCGAAGATAATGTTGTCG+AGG + chr3.3:17839521-17839540 MS.gene28231:CDS 45.0%
TGTTGTCGAGGTTGGATTAC+AGG + chr3.3:17839533-17839552 MS.gene28231:CDS 45.0%
TTAACCAAGGCAGAGAAAGC+AGG + chr3.3:17839318-17839337 MS.gene28231:CDS 45.0%
TTTGTCCCCAAAAAACAGGC+TGG - chr3.3:17839155-17839174 None:intergenic 45.0%
! ACACGGACGAAGTGTTAAGT+AGG - chr3.3:17838479-17838498 None:intergenic 45.0%
! CAAGTTCTTCGGCTTTTGAG+AGG - chr3.3:17839404-17839423 None:intergenic 45.0%
! CACGGACGAAGTGTTAAGTA+GGG - chr3.3:17838478-17838497 None:intergenic 45.0%
! GAGACAGAAGGAACTGGTTT+GGG - chr3.3:17838015-17838034 None:intergenic 45.0%
! GCTTTGATCTGTGTTGTTGG+TGG + chr3.3:17839564-17839583 MS.gene28231:CDS 45.0%
! GGTTGTCGTCGGTATTTCTA+CGG + chr3.3:17839073-17839092 MS.gene28231:intron 45.0%
!!! CGTAGAACCAGCCTGTTTTT+TGG + chr3.3:17839145-17839164 MS.gene28231:intron 45.0%
CGATAATCCTGCTTTCTCGG+CGG - chr3.3:17839355-17839374 None:intergenic 50.0%
CTGAGTGTGAAGAGTGATCC+TGG - chr3.3:17839459-17839478 None:intergenic 50.0%
GACGACAACCATGTCACTCT+TGG - chr3.3:17839063-17839082 None:intergenic 50.0%
GCTACTGATCCATCAACTCC+AGG + chr3.3:17839438-17839457 MS.gene28231:CDS 50.0%
GTCGATGTTACAGCACTCCT+AGG - chr3.3:17839255-17839274 None:intergenic 50.0%
TCCACCTAGATAGGTGTCCA+CGG + chr3.3:17838788-17838807 MS.gene28231:intron 50.0%
TCCTAGGTTGTTCCCCAAGT+GGG - chr3.3:17839239-17839258 None:intergenic 50.0%
!! GTGAGTGTGAGTTTTGTCGC+TGG + chr3.3:17838497-17838516 MS.gene28231:intron 50.0%
!! TGTGTTGTTGGTGGTTCTGC+TGG + chr3.3:17839573-17839592 MS.gene28231:CDS 50.0%
ACCGTGGACACCTATCTAGG+TGG - chr3.3:17838792-17838811 None:intergenic 55.0%
ACCTAGATAGGTGTCCACGG+TGG + chr3.3:17838791-17838810 MS.gene28231:intron 55.0%
CGAGGTTGGATTACAGGCTG+TGG + chr3.3:17839539-17839558 MS.gene28231:CDS 55.0%
CTACGGTGGTCCACCTAGAT+AGG + chr3.3:17838779-17838798 MS.gene28231:intron 55.0%
CTCCTAGGTTGTTCCCCAAG+TGG - chr3.3:17839240-17839259 None:intergenic 55.0%
GAGTGAGACGGTGACTCGAT+TGG + chr3.3:17838895-17838914 MS.gene28231:intron 55.0%
TCCACCGTGGACACCTATCT+AGG - chr3.3:17838795-17838814 None:intergenic 55.0%
TCCCACTTGGGGAACAACCT+AGG + chr3.3:17839235-17839254 MS.gene28231:CDS 55.0%
TTATCAGCCGCCGAGAAAGC+AGG + chr3.3:17839345-17839364 MS.gene28231:CDS 55.0%
!!! GGTGGTTCTGCTGGTTTTGC+TGG + chr3.3:17839582-17839601 MS.gene28231:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 17838003 17839641 17838003 ID=MS.gene28231
chr3.3 mRNA 17838003 17839641 17838003 ID=MS.gene28231.t1;Parent=MS.gene28231
chr3.3 exon 17838003 17838056 17838003 ID=MS.gene28231.t1.exon1;Parent=MS.gene28231.t1
chr3.3 CDS 17838003 17838056 17838003 ID=cds.MS.gene28231.t1;Parent=MS.gene28231.t1
chr3.3 exon 17839222 17839641 17839222 ID=MS.gene28231.t1.exon2;Parent=MS.gene28231.t1
chr3.3 CDS 17839222 17839641 17839222 ID=cds.MS.gene28231.t1;Parent=MS.gene28231.t1
Gene Sequence

>MS.gene28231

ATGGCTACTCCCAAACCAGTTCCTTCTGTCTCTAAAACTGTAGCTTCTAAAAAGGTCAGTGCAAAACATTAACCATATAATTCTTACTTGTTATTTTGATTTTTGATGATTTACATAATTGCTTTGATTCTTTTATGCAATATTATGCTGTGTGGAACTGGAACATGTCTATAATATATTTACCAATTTAATTATGAGAAAGGATAAAAAAAAACTAGCTCTTATAATATTATAAAACTTATCTGTGAAATTTAACAATGTGTGAAAAGCATTGAACACTAGTTTTAATGCTCTTTGATGATATGGTATATGGCCTAGGTATACATAAACTGTAATTTGTCATAGGGGTCTGTAGTTAATTGATCATTAATAAGCAGACTTGTATAAAACTTATGTCCGATAAGTACAACTCATATGGTAAAAGGCTGAGGGGACATTTGATATGCTGAGACGCGGGTTTGAACTCACAGTTCCCTACTTAACACTTCGTCCGTGTGAGTGTGAGTTTTGTCGCTGGAATCTTTAGGAAAAAAAAGGAACTATACTGTAAAAAAATCACCTGGTTATTTTACGTTTTCAATAATTTAATCATTAACCTACATAGAAACTTTACTTTATCAGGCTTTTAAGTAGGCTAATACGTCAGACCTTCGAAAAGGTCTGATAAGTACTAAAAATGGCGTATGATAGTGAACTTATCATGTTTATAATTTAAGTTTTTTGACAGACCAGACCTATTTAAACAAAGCATTTCCAAACATGGCATTATGTAAATTCTACGGTGGTCCACCTAGATAGGTGTCCACGGTGGATTAACACTTGAAAATAACCTGAAATTACGACTCATAGTACATGTCGAAAGCAAAAAATTCACAATCCTCTTTGTCTTAATGAGTGAGACGGTGACTCGATTGGTACTAGTTCGAAAAGACATCTGGGAAAACGAACGAGTCAACAAAACTCAACTCATATTTGTTTTCCTTTGTTTTGGTAGAACAAACATCAATCAAGTCACTGTATCACCTATGACAATAACAATGACTGTAATTTTCTACTTCCAAGAGTGACATGGTTGTCGTCGGTATTTCTACGGTAATTTTCAAGTGGTGGATAACCATAAACCGTTTATTAAAGTGGTCTACCGTAGAACCAGCCTGTTTTTTGGGGACAAAAGTTGGATTTTTTTCTTACAATTACTAAGTCACGAGCACAATTGCAGAATTCAACTGTATTCCCACTTGGGGAACAACCTAGGAGTGCTGTAACATCGACACAATCCGTTAAACTCCTCACAAGAATGGAACAACTGAGACTGTTAACCAAGGCAGAGAAAGCAGGTTTATTATCAGCCGCCGAGAAAGCAGGATTATCGCTATCAACAATAGAGAAACTTGGTCTCCTCTCAAAAGCCGAAGAACTTGGTGTTTTATCAGCTGCTACTGATCCATCAACTCCAGGATCACTCTTCACACTCAGCTTTGTTTTACTTCTTTTAGGTCCTTTGTTTGTTTATCTTGTTCCCGAAGATAATGTTGTCGAGGTTGGATTACAGGCTGTGGTTGCTTTGATCTGTGTTGTTGGTGGTTCTGCTGGTTTTGCTGGTTCAAGTTTTGTATCCAACTTGCAGAAAATAAAATAA

Protein sequence

>MS.gene28231.t1

MATPKPVPSVSKTVASKKNSTVFPLGEQPRSAVTSTQSVKLLTRMEQLRLLTKAEKAGLLSAAEKAGLSLSTIEKLGLLSKAEELGVLSAATDPSTPGSLFTLSFVLLLLGPLFVYLVPEDNVVEVGLQAVVALICVVGGSAGFAGSSFVSNLQKIK