Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28514.t1 | XP_013446424.1 | 88.2 | 490 | 48 | 4 | 1 | 480 | 1 | 490 | 4.80E-201 | 710.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28514.t1 | O64827 | 25.9 | 517 | 270 | 11 | 43 | 448 | 409 | 923 | 1.7e-29 | 132.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28514.t1 | A0A072TTG4 | 88.2 | 490 | 48 | 4 | 1 | 480 | 1 | 490 | 3.4e-201 | 710.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene28514.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene28514 | 0.803173 | 3.95E-49 | -1.69E-46 |
| MS.gene049272 | MS.gene28514 | 0.813395 | 2.62E-51 | -1.69E-46 |
| MS.gene049825 | MS.gene28514 | 0.826119 | 3.23E-54 | -1.69E-46 |
| MS.gene049873 | MS.gene28514 | 0.826719 | 2.32E-54 | -1.69E-46 |
| MS.gene050156 | MS.gene28514 | 0.82117 | 4.66E-53 | -1.69E-46 |
| MS.gene050201 | MS.gene28514 | 0.818788 | 1.63E-52 | -1.69E-46 |
| MS.gene050227 | MS.gene28514 | 0.803965 | 2.71E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene28514 | 0.810786 | 9.70E-51 | -1.69E-46 |
| MS.gene050303 | MS.gene28514 | 0.82695 | 2.05E-54 | -1.69E-46 |
| MS.gene050355 | MS.gene28514 | 0.820993 | 5.11E-53 | -1.69E-46 |
| MS.gene050442 | MS.gene28514 | 0.808053 | 3.74E-50 | -1.69E-46 |
| MS.gene050719 | MS.gene28514 | 0.852537 | 4.33E-61 | -1.69E-46 |
| MS.gene050721 | MS.gene28514 | 0.852693 | 3.90E-61 | -1.69E-46 |
| MS.gene050724 | MS.gene28514 | 0.820985 | 5.13E-53 | -1.69E-46 |
| MS.gene050780 | MS.gene28514 | 0.839813 | 1.27E-57 | -1.69E-46 |
| MS.gene050824 | MS.gene28514 | 0.814885 | 1.23E-51 | -1.69E-46 |
| MS.gene050844 | MS.gene28514 | 0.812066 | 5.11E-51 | -1.69E-46 |
| MS.gene051207 | MS.gene28514 | 0.809131 | 2.20E-50 | -1.69E-46 |
| MS.gene051247 | MS.gene28514 | 0.828183 | 1.04E-54 | -1.69E-46 |
| MS.gene051744 | MS.gene28514 | 0.801103 | 1.05E-48 | -1.69E-46 |
| MS.gene051935 | MS.gene28514 | 0.803519 | 3.35E-49 | -1.69E-46 |
| MS.gene051957 | MS.gene28514 | 0.810275 | 1.25E-50 | -1.69E-46 |
| MS.gene052012 | MS.gene28514 | 0.802432 | 5.63E-49 | -1.69E-46 |
| MS.gene052020 | MS.gene28514 | 0.803376 | 3.59E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene28514 | 0.819578 | 1.08E-52 | -1.69E-46 |
| MS.gene052190 | MS.gene28514 | 0.823971 | 1.04E-53 | -1.69E-46 |
| MS.gene052208 | MS.gene28514 | 0.823206 | 1.57E-53 | -1.69E-46 |
| MS.gene052333 | MS.gene28514 | 0.811112 | 8.24E-51 | -1.69E-46 |
| MS.gene052358 | MS.gene28514 | -0.841982 | 3.41E-58 | -1.69E-46 |
| MS.gene052519 | MS.gene28514 | 0.811732 | 6.04E-51 | -1.69E-46 |
| MS.gene052582 | MS.gene28514 | 0.807454 | 5.01E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene28514 | 0.815017 | 1.15E-51 | -1.69E-46 |
| MS.gene052586 | MS.gene28514 | 0.824399 | 8.24E-54 | -1.69E-46 |
| MS.gene052773 | MS.gene28514 | 0.808264 | 3.37E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene28514 | 0.809475 | 1.86E-50 | -1.69E-46 |
| MS.gene052896 | MS.gene28514 | 0.826607 | 2.47E-54 | -1.69E-46 |
| MS.gene053006 | MS.gene28514 | 0.813691 | 2.25E-51 | -1.69E-46 |
| MS.gene053054 | MS.gene28514 | -0.815118 | 1.09E-51 | -1.69E-46 |
| MS.gene053221 | MS.gene28514 | 0.821233 | 4.50E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene28514 | 0.820405 | 6.98E-53 | -1.69E-46 |
| MS.gene053883 | MS.gene28514 | 0.811762 | 5.95E-51 | -1.69E-46 |
| MS.gene053953 | MS.gene28514 | 0.808563 | 2.91E-50 | -1.69E-46 |
| MS.gene053966 | MS.gene28514 | 0.815173 | 1.06E-51 | -1.69E-46 |
| MS.gene05414 | MS.gene28514 | 0.81752 | 3.16E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene28514 | 0.824771 | 6.74E-54 | -1.69E-46 |
| MS.gene054291 | MS.gene28514 | -0.815759 | 7.84E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene28514 | 0.836 | 1.21E-56 | -1.69E-46 |
| MS.gene054658 | MS.gene28514 | 0.803142 | 4.01E-49 | -1.69E-46 |
| MS.gene05468 | MS.gene28514 | 0.802296 | 6.00E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene28514 | 0.806108 | 9.65E-50 | -1.69E-46 |
| MS.gene055778 | MS.gene28514 | 0.844714 | 6.37E-59 | -1.69E-46 |
| MS.gene055818 | MS.gene28514 | 0.839319 | 1.70E-57 | -1.69E-46 |
| MS.gene056091 | MS.gene28514 | 0.81271 | 3.70E-51 | -1.69E-46 |
| MS.gene056498 | MS.gene28514 | 0.800861 | 1.18E-48 | -1.69E-46 |
| MS.gene056506 | MS.gene28514 | 0.830809 | 2.38E-55 | -1.69E-46 |
| MS.gene057026 | MS.gene28514 | 0.851008 | 1.17E-60 | -1.69E-46 |
| MS.gene057027 | MS.gene28514 | 0.801377 | 9.27E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene28514 | 0.819958 | 8.84E-53 | -1.69E-46 |
| MS.gene057414 | MS.gene28514 | 0.806606 | 7.58E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene28514 | 0.809791 | 1.59E-50 | -1.69E-46 |
| MS.gene057915 | MS.gene28514 | 0.805499 | 1.30E-49 | -1.69E-46 |
| MS.gene057967 | MS.gene28514 | 0.807656 | 4.54E-50 | -1.69E-46 |
| MS.gene057980 | MS.gene28514 | 0.81514 | 1.08E-51 | -1.69E-46 |
| MS.gene058037 | MS.gene28514 | 0.815414 | 9.36E-52 | -1.69E-46 |
| MS.gene058625 | MS.gene28514 | 0.825598 | 4.30E-54 | -1.69E-46 |
| MS.gene058636 | MS.gene28514 | 0.825638 | 4.20E-54 | -1.69E-46 |
| MS.gene058829 | MS.gene28514 | 0.821503 | 3.90E-53 | -1.69E-46 |
| MS.gene058979 | MS.gene28514 | 0.810913 | 9.10E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene28514 | 0.833764 | 4.42E-56 | -1.69E-46 |
| MS.gene059789 | MS.gene28514 | 0.835015 | 2.15E-56 | -1.69E-46 |
| MS.gene060322 | MS.gene28514 | 0.813525 | 2.45E-51 | -1.69E-46 |
| MS.gene060521 | MS.gene28514 | 0.813798 | 2.13E-51 | -1.69E-46 |
| MS.gene060553 | MS.gene28514 | 0.806616 | 7.54E-50 | -1.69E-46 |
| MS.gene060572 | MS.gene28514 | 0.861401 | 1.05E-63 | -1.69E-46 |
| MS.gene060586 | MS.gene28514 | 0.802973 | 4.35E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene28514 | -0.817636 | 2.98E-52 | -1.69E-46 |
| MS.gene060683 | MS.gene28514 | 0.808473 | 3.04E-50 | -1.69E-46 |
| MS.gene060685 | MS.gene28514 | 0.808854 | 2.52E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene28514 | -0.867274 | 1.54E-65 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28514.t1 | MTR_8g078700 | 87.347 | 490 | 52 | 4 | 1 | 480 | 1 | 490 | 0.0 | 873 |
| MS.gene28514.t1 | MTR_8g078490 | 62.039 | 461 | 148 | 7 | 33 | 475 | 387 | 838 | 0.0 | 544 |
| MS.gene28514.t1 | MTR_4g132610 | 59.236 | 314 | 103 | 3 | 39 | 345 | 367 | 662 | 2.70e-114 | 361 |
| MS.gene28514.t1 | MTR_5g018850 | 58.271 | 266 | 100 | 5 | 218 | 475 | 817 | 1079 | 3.77e-91 | 304 |
| MS.gene28514.t1 | MTR_5g018850 | 41.096 | 146 | 79 | 1 | 56 | 194 | 507 | 652 | 1.50e-26 | 114 |
| MS.gene28514.t1 | MTR_8g078505 | 58.943 | 246 | 53 | 2 | 20 | 228 | 6 | 240 | 2.77e-85 | 272 |
| MS.gene28514.t1 | MTR_4g132670 | 42.683 | 246 | 73 | 4 | 78 | 322 | 210 | 388 | 2.03e-50 | 177 |
| MS.gene28514.t1 | MTR_4g132670 | 56.897 | 58 | 25 | 0 | 58 | 115 | 55 | 112 | 6.34e-14 | 73.9 |
| MS.gene28514.t1 | MTR_8g442480 | 51.685 | 89 | 40 | 2 | 63 | 148 | 2 | 90 | 4.01e-20 | 85.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28514.t1 | AT2G23740 | 50.254 | 197 | 96 | 2 | 252 | 448 | 722 | 916 | 4.40e-55 | 199 |
| MS.gene28514.t1 | AT2G23740 | 40.602 | 133 | 71 | 2 | 45 | 169 | 404 | 536 | 8.84e-20 | 93.6 |
| MS.gene28514.t1 | AT2G23740 | 50.254 | 197 | 96 | 2 | 252 | 448 | 729 | 923 | 4.80e-55 | 199 |
| MS.gene28514.t1 | AT2G23740 | 40.602 | 133 | 71 | 2 | 45 | 169 | 411 | 543 | 9.59e-20 | 93.6 |
| MS.gene28514.t1 | AT2G23740 | 50.254 | 197 | 96 | 2 | 252 | 448 | 729 | 923 | 4.80e-55 | 199 |
| MS.gene28514.t1 | AT2G23740 | 40.602 | 133 | 71 | 2 | 45 | 169 | 411 | 543 | 9.59e-20 | 93.6 |
| MS.gene28514.t1 | AT2G23740 | 50.254 | 197 | 96 | 2 | 252 | 448 | 729 | 923 | 4.80e-55 | 199 |
| MS.gene28514.t1 | AT2G23740 | 40.602 | 133 | 71 | 2 | 45 | 169 | 411 | 543 | 9.59e-20 | 93.6 |
| MS.gene28514.t1 | AT2G23740 | 50.254 | 197 | 96 | 2 | 252 | 448 | 729 | 923 | 5.06e-55 | 199 |
| MS.gene28514.t1 | AT2G23740 | 40.602 | 133 | 71 | 2 | 45 | 169 | 411 | 543 | 8.79e-20 | 93.6 |
| MS.gene28514.t1 | AT2G23740 | 50.254 | 197 | 96 | 2 | 252 | 448 | 729 | 923 | 5.06e-55 | 199 |
| MS.gene28514.t1 | AT2G23740 | 40.602 | 133 | 71 | 2 | 45 | 169 | 411 | 543 | 8.79e-20 | 93.6 |
Find 91 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTTCAGATAGAGCCTTTC+TGG | 0.146763 | 8.3:-25654145 | None:intergenic |
| TCCAAACCTCTTTCAAATTT+AGG | 0.154205 | 8.3:-25653958 | None:intergenic |
| GGCGAAACGCTCAAGCTTTC+CGG | 0.154625 | 8.3:+25653280 | MS.gene28514:CDS |
| ATTCCTTGTGGTTGTCATTT+TGG | 0.199979 | 8.3:+25653592 | MS.gene28514:CDS |
| CCAGCCTCATGCAGATAAAT+TGG | 0.273277 | 8.3:+25654074 | MS.gene28514:CDS |
| TTGGAAATATAGAGGAATTA+TGG | 0.274132 | 8.3:+25653611 | MS.gene28514:CDS |
| GGCAAATAAGTTGCTTGAAA+TGG | 0.279485 | 8.3:+25654038 | MS.gene28514:CDS |
| CCTCTTTCAAATTTAGGATT+AGG | 0.281407 | 8.3:-25653952 | None:intergenic |
| TTCTTCGCAAAGAACATCTT+TGG | 0.285228 | 8.3:+25653259 | MS.gene28514:CDS |
| ACGCAAATATGTTCTTCATT+AGG | 0.299340 | 8.3:-25652764 | None:intergenic |
| CTTGACATTGTCGACCCTTT+TGG | 0.303194 | 8.3:-25652871 | None:intergenic |
| AGACTTGGTGTTTGTACATC+TGG | 0.329602 | 8.3:+25653186 | MS.gene28514:CDS |
| TCCTAAATTTGAAAGAGGTT+TGG | 0.365758 | 8.3:+25653957 | MS.gene28514:CDS |
| CCATCATCAAGCTGCTCATA+TGG | 0.373746 | 8.3:+25653846 | MS.gene28514:CDS |
| GCCTGTCCACTGATCATTAC+CGG | 0.388651 | 8.3:-25653299 | None:intergenic |
| GCAGTTGTACCTCCACAAAA+TGG | 0.390067 | 8.3:-25653004 | None:intergenic |
| TCCAATGGTTGCCAAGTGTT+TGG | 0.392168 | 8.3:-25653417 | None:intergenic |
| GCAAATAAGTTGCTTGAAAT+GGG | 0.399729 | 8.3:+25654039 | MS.gene28514:CDS |
| AAGAGACACTATGGGCAGTT+TGG | 0.400556 | 8.3:+25653544 | MS.gene28514:CDS |
| AGTCTTTGCAAATCAGGCTC+TGG | 0.401375 | 8.3:-25653168 | None:intergenic |
| CGATCTAGCCAGCAGGCGCT+TGG | 0.413605 | 8.3:+25653873 | MS.gene28514:CDS |
| ACACCAAGTCTTTGCAAATC+AGG | 0.417723 | 8.3:-25653174 | None:intergenic |
| GGAAGAATCTCAATGCTTAG+AGG | 0.420258 | 8.3:+25654095 | MS.gene28514:CDS |
| AGTTATCGAAAGCTTAAATT+CGG | 0.424504 | 8.3:-25653659 | None:intergenic |
| CCTAATCCTAAATTTGAAAG+AGG | 0.427935 | 8.3:+25653952 | MS.gene28514:CDS |
| GTTTGGAAGAAGCATCAAAT+AGG | 0.428853 | 8.3:+25653974 | MS.gene28514:CDS |
| ATCGAAAGCTTAAATTCGGC+AGG | 0.430592 | 8.3:-25653655 | None:intergenic |
| TTTCATGACTTTCCATCCAA+TGG | 0.430775 | 8.3:-25653432 | None:intergenic |
| CCAATTTATCTGCATGAGGC+TGG | 0.431124 | 8.3:-25654074 | None:intergenic |
| ACTCTTTGCCAAGCGCCTGC+TGG | 0.443750 | 8.3:-25653881 | None:intergenic |
| CAGTGACAGTTCATCTGTAA+TGG | 0.451819 | 8.3:+25652829 | MS.gene28514:CDS |
| GTTCGCGGAAGTTTAGTTGC+AGG | 0.454226 | 8.3:+25653782 | MS.gene28514:CDS |
| TAATTTCAAGAATAGAAATC+AGG | 0.462085 | 8.3:+25654017 | MS.gene28514:CDS |
| TTCTCCTAAAATGATTGAGC+AGG | 0.465200 | 8.3:+25653714 | MS.gene28514:CDS |
| TGATGATGGCGACCGAGATC+AGG | 0.469915 | 8.3:-25653832 | None:intergenic |
| GTCTTTGCAAATCAGGCTCT+GGG | 0.470035 | 8.3:-25653167 | None:intergenic |
| ACCAAACACTTGGCAACCAT+TGG | 0.477256 | 8.3:+25653416 | MS.gene28514:CDS |
| GACTCTTATAGAAAACCAAA+AGG | 0.481717 | 8.3:+25652856 | MS.gene28514:CDS |
| CCATATGAGCAGCTTGATGA+TGG | 0.488631 | 8.3:-25653846 | None:intergenic |
| TGAGAGTGCTGTTAAGTGTA+AGG | 0.490329 | 8.3:+25653369 | MS.gene28514:CDS |
| TTGGATGGAAAGTCATGAAA+AGG | 0.491304 | 8.3:+25653435 | MS.gene28514:CDS |
| TCATCATTGACGATTGACTC+TGG | 0.492547 | 8.3:-25653229 | None:intergenic |
| AGTGAAGGAATCAAAACAAA+AGG | 0.493161 | 8.3:-25653491 | None:intergenic |
| CATGTTCAGAAGAGACACTA+TGG | 0.495523 | 8.3:+25653535 | MS.gene28514:CDS |
| ACATCTGCACAAAGCTGAGA+TGG | 0.500567 | 8.3:+25653081 | MS.gene28514:CDS |
| CAGTGCGTGAGAACCGCAAT+TGG | 0.508352 | 8.3:+25652917 | MS.gene28514:CDS |
| GTTTCGTTTATCTACCAGAA+AGG | 0.508712 | 8.3:+25654131 | MS.gene28514:CDS |
| TATGCTCAGAGACTAAAATC+TGG | 0.515386 | 8.3:+25653916 | MS.gene28514:CDS |
| GAGCCTGATTTGCAAAGACT+TGG | 0.521361 | 8.3:+25653171 | MS.gene28514:CDS |
| GAGATATTGAATTTGAGCTC+TGG | 0.522387 | 8.3:-25653105 | None:intergenic |
| TTCAAATTTAGGATTAGGAC+GGG | 0.522702 | 8.3:-25653947 | None:intergenic |
| ATTAGCTTTAGCCCTTCTCC+TGG | 0.523241 | 8.3:-25653998 | None:intergenic |
| TCCGGTAATGATCAGTGGAC+AGG | 0.523358 | 8.3:+25653298 | MS.gene28514:CDS |
| TGGAGCGCGATCTAGCCAGC+AGG | 0.523628 | 8.3:+25653866 | MS.gene28514:CDS |
| AGCAGGCGCTTGGCAAAGAG+TGG | 0.523735 | 8.3:+25653883 | MS.gene28514:CDS |
| TGTACATCTGGAAAGTCCAT+TGG | 0.523941 | 8.3:+25653198 | MS.gene28514:CDS |
| ACAAATCAAGGAGCAGCTTA+CGG | 0.524363 | 8.3:+25652740 | MS.gene28514:CDS |
| CAATATCTCGTACATTAAAG+AGG | 0.524991 | 8.3:+25653119 | MS.gene28514:CDS |
| GACTCTGGATTGATCTCCAA+TGG | 0.529884 | 8.3:-25653214 | None:intergenic |
| CTGAGAATCGAAGTGGTTCG+CGG | 0.530378 | 8.3:+25653767 | MS.gene28514:CDS |
| GTGTATATTGTTACCAATTG+CGG | 0.530995 | 8.3:-25652930 | None:intergenic |
| CATCAAATAGGACCAGGAGA+AGG | 0.534319 | 8.3:+25653986 | MS.gene28514:CDS |
| TTTGATTTACTACCTGATCT+CGG | 0.536142 | 8.3:+25653820 | MS.gene28514:CDS |
| AACACTTGGCAACCATTGGA+TGG | 0.536290 | 8.3:+25653420 | MS.gene28514:CDS |
| TTTCAAATTTAGGATTAGGA+CGG | 0.543514 | 8.3:-25653948 | None:intergenic |
| ATCAAATAGGACCAGGAGAA+GGG | 0.546017 | 8.3:+25653987 | MS.gene28514:CDS |
| TTTATCTGCATGAGGCTGGA+TGG | 0.546716 | 8.3:-25654070 | None:intergenic |
| TCTTCCAATTTATCTGCATG+AGG | 0.563398 | 8.3:-25654078 | None:intergenic |
| GATTGCTCTGAGACAAATCA+AGG | 0.567116 | 8.3:+25652728 | MS.gene28514:CDS |
| GACAGTTCATCTGTAATGGA+AGG | 0.570153 | 8.3:+25652833 | MS.gene28514:CDS |
| ACCAGAAAGGCTCTATCTGA+AGG | 0.579442 | 8.3:+25654144 | MS.gene28514:CDS |
| CATCTGCACAAAGCTGAGAT+GGG | 0.579947 | 8.3:+25653082 | MS.gene28514:CDS |
| ACTCTTATAGAAAACCAAAA+GGG | 0.580021 | 8.3:+25652857 | MS.gene28514:CDS |
| TGGACAGGCTCTCCTAGCTA+TGG | 0.583120 | 8.3:+25653313 | MS.gene28514:CDS |
| TTATGATCATTACCATAGCT+AGG | 0.583706 | 8.3:-25653325 | None:intergenic |
| TGTGGAGGTACAACTGCTGC+TGG | 0.586893 | 8.3:+25653010 | MS.gene28514:CDS |
| TCTCCTAAAATGATTGAGCA+GGG | 0.608885 | 8.3:+25653715 | MS.gene28514:CDS |
| GAAGCACAGTGGTTGTTCAG+AGG | 0.608970 | 8.3:+25653457 | MS.gene28514:CDS |
| GATAATTCTGAGAATCGAAG+TGG | 0.614192 | 8.3:+25653760 | MS.gene28514:CDS |
| AGCTTTCCGGTAATGATCAG+TGG | 0.616263 | 8.3:+25653293 | MS.gene28514:CDS |
| CATATATCGAAGACAAGGGA+AGG | 0.617551 | 8.3:+25652894 | MS.gene28514:CDS |
| GAGCAACTAACATTGTCTAC+CGG | 0.626990 | 8.3:+25653685 | MS.gene28514:CDS |
| TCAAGCATATATCGAAGACA+AGG | 0.630094 | 8.3:+25652889 | MS.gene28514:CDS |
| ACAGTTCATCTGTAATGGAA+GGG | 0.632090 | 8.3:+25652834 | MS.gene28514:CDS |
| AAGAAGCATCAAATAGGACC+AGG | 0.634670 | 8.3:+25653980 | MS.gene28514:CDS |
| ATGTTCAGAAGAGACACTAT+GGG | 0.654163 | 8.3:+25653536 | MS.gene28514:CDS |
| TCGAAAGCTTAAATTCGGCA+GGG | 0.657128 | 8.3:-25653654 | None:intergenic |
| AAAGCTGAACAAAATTGCAG+TGG | 0.668916 | 8.3:+25653142 | MS.gene28514:CDS |
| CAAGCATATATCGAAGACAA+GGG | 0.692685 | 8.3:+25652890 | MS.gene28514:CDS |
| TTTCCAAAATGACAACCACA+AGG | 0.715599 | 8.3:-25653595 | None:intergenic |
| GTCATGAAAAGGAAGCACAG+TGG | 0.767507 | 8.3:+25653446 | MS.gene28514:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTCTTAAGTAAAAAAGAA+AGG | + | chr8.3:25652963-25652982 | MS.gene28514:CDS | 15.0% |
| !! | TAATTTCAAGAATAGAAATC+AGG | + | chr8.3:25654017-25654036 | MS.gene28514:CDS | 20.0% |
| !! | TTCTTACAAAAAGATAATCT+GGG | - | chr8.3:25653061-25653080 | None:intergenic | 20.0% |
| !! | TTTCTTACAAAAAGATAATC+TGG | - | chr8.3:25653062-25653081 | None:intergenic | 20.0% |
| ! | ACTCTTATAGAAAACCAAAA+GGG | + | chr8.3:25652857-25652876 | MS.gene28514:CDS | 25.0% |
| ! | AGTTATCGAAAGCTTAAATT+CGG | - | chr8.3:25653662-25653681 | None:intergenic | 25.0% |
| ! | CTTCACTAACAAAAAACTAT+TGG | + | chr8.3:25653507-25653526 | MS.gene28514:CDS | 25.0% |
| ! | TTGGAAATATAGAGGAATTA+TGG | + | chr8.3:25653611-25653630 | MS.gene28514:CDS | 25.0% |
| !! | TTTCAAATTTAGGATTAGGA+CGG | - | chr8.3:25653951-25653970 | None:intergenic | 25.0% |
| !!! | CAATAGTTTTTTGTTAGTGA+AGG | - | chr8.3:25653509-25653528 | None:intergenic | 25.0% |
| !!! | TTGTCATTTTGGAAATATAG+AGG | + | chr8.3:25653603-25653622 | MS.gene28514:CDS | 25.0% |
| ACAAAAAGATAATCTGGGAA+GGG | - | chr8.3:25653056-25653075 | None:intergenic | 30.0% | |
| ACGCAAATATGTTCTTCATT+AGG | - | chr8.3:25652767-25652786 | None:intergenic | 30.0% | |
| AGTGAAGGAATCAAAACAAA+AGG | - | chr8.3:25653494-25653513 | None:intergenic | 30.0% | |
| CAATATCTCGTACATTAAAG+AGG | + | chr8.3:25653119-25653138 | MS.gene28514:CDS | 30.0% | |
| CCTAATCCTAAATTTGAAAG+AGG | + | chr8.3:25653952-25653971 | MS.gene28514:CDS | 30.0% | |
| GACTCTTATAGAAAACCAAA+AGG | + | chr8.3:25652856-25652875 | MS.gene28514:CDS | 30.0% | |
| GCAAATAAGTTGCTTGAAAT+GGG | + | chr8.3:25654039-25654058 | MS.gene28514:CDS | 30.0% | |
| GTGTATATTGTTACCAATTG+CGG | - | chr8.3:25652933-25652952 | None:intergenic | 30.0% | |
| TACAAAAAGATAATCTGGGA+AGG | - | chr8.3:25653057-25653076 | None:intergenic | 30.0% | |
| TCCAAACCTCTTTCAAATTT+AGG | - | chr8.3:25653961-25653980 | None:intergenic | 30.0% | |
| TCCTAAATTTGAAAGAGGTT+TGG | + | chr8.3:25653957-25653976 | MS.gene28514:CDS | 30.0% | |
| TTATGATCATTACCATAGCT+AGG | - | chr8.3:25653328-25653347 | None:intergenic | 30.0% | |
| ! | CCTCTTTCAAATTTAGGATT+AGG | - | chr8.3:25653955-25653974 | None:intergenic | 30.0% |
| ! | CTTTTTCTATGTATTCCTTG+TGG | + | chr8.3:25653580-25653599 | MS.gene28514:CDS | 30.0% |
| ! | TTCAAATTTAGGATTAGGAC+GGG | - | chr8.3:25653950-25653969 | None:intergenic | 30.0% |
| ! | TTTGATTTACTACCTGATCT+CGG | + | chr8.3:25653820-25653839 | MS.gene28514:CDS | 30.0% |
| ! | TTTTCTGATAACCAAACACT+TGG | + | chr8.3:25653406-25653425 | MS.gene28514:CDS | 30.0% |
| AAAGCTGAACAAAATTGCAG+TGG | + | chr8.3:25653142-25653161 | MS.gene28514:CDS | 35.0% | |
| ATGTTCAGAAGAGACACTAT+GGG | + | chr8.3:25653536-25653555 | MS.gene28514:CDS | 35.0% | |
| CAAGCATATATCGAAGACAA+GGG | + | chr8.3:25652890-25652909 | MS.gene28514:CDS | 35.0% | |
| GAGATATTGAATTTGAGCTC+TGG | - | chr8.3:25653108-25653127 | None:intergenic | 35.0% | |
| GGCAAATAAGTTGCTTGAAA+TGG | + | chr8.3:25654038-25654057 | MS.gene28514:CDS | 35.0% | |
| GTTTCGTTTATCTACCAGAA+AGG | + | chr8.3:25654131-25654150 | MS.gene28514:CDS | 35.0% | |
| TATGCTCAGAGACTAAAATC+TGG | + | chr8.3:25653916-25653935 | MS.gene28514:CDS | 35.0% | |
| TCAAGCATATATCGAAGACA+AGG | + | chr8.3:25652889-25652908 | MS.gene28514:CDS | 35.0% | |
| TCTCCTAAAATGATTGAGCA+GGG | + | chr8.3:25653715-25653734 | MS.gene28514:CDS | 35.0% | |
| TCTTCCAATTTATCTGCATG+AGG | - | chr8.3:25654081-25654100 | None:intergenic | 35.0% | |
| TTCTCCTAAAATGATTGAGC+AGG | + | chr8.3:25653714-25653733 | MS.gene28514:CDS | 35.0% | |
| TTCTTCGCAAAGAACATCTT+TGG | + | chr8.3:25653259-25653278 | MS.gene28514:CDS | 35.0% | |
| TTGGATGGAAAGTCATGAAA+AGG | + | chr8.3:25653435-25653454 | MS.gene28514:CDS | 35.0% | |
| TTTCATGACTTTCCATCCAA+TGG | - | chr8.3:25653435-25653454 | None:intergenic | 35.0% | |
| TTTCCAAAATGACAACCACA+AGG | - | chr8.3:25653598-25653617 | None:intergenic | 35.0% | |
| ! | AAAGCAATCACTCCATTTTG+TGG | + | chr8.3:25652992-25653011 | MS.gene28514:CDS | 35.0% |
| ! | ACAGTTCATCTGTAATGGAA+GGG | + | chr8.3:25652834-25652853 | MS.gene28514:CDS | 35.0% |
| ! | GATAATTCTGAGAATCGAAG+TGG | + | chr8.3:25653760-25653779 | MS.gene28514:CDS | 35.0% |
| ! | TTTCCCTGCTCAATCATTTT+AGG | - | chr8.3:25653721-25653740 | None:intergenic | 35.0% |
| !! | ATTCCTTGTGGTTGTCATTT+TGG | + | chr8.3:25653592-25653611 | MS.gene28514:CDS | 35.0% |
| !! | CATTTTAGGAGAATCATCAC+CGG | - | chr8.3:25653707-25653726 | None:intergenic | 35.0% |
| !! | GTTTGGAAGAAGCATCAAAT+AGG | + | chr8.3:25653974-25653993 | MS.gene28514:CDS | 35.0% |
| ACAAATCAAGGAGCAGCTTA+CGG | + | chr8.3:25652740-25652759 | MS.gene28514:CDS | 40.0% | |
| ACACCAAGTCTTTGCAAATC+AGG | - | chr8.3:25653177-25653196 | None:intergenic | 40.0% | |
| ATCAAATAGGACCAGGAGAA+GGG | + | chr8.3:25653987-25654006 | MS.gene28514:CDS | 40.0% | |
| ATCGAAAGCTTAAATTCGGC+AGG | - | chr8.3:25653658-25653677 | None:intergenic | 40.0% | |
| CAGTGACAGTTCATCTGTAA+TGG | + | chr8.3:25652829-25652848 | MS.gene28514:CDS | 40.0% | |
| CATATATCGAAGACAAGGGA+AGG | + | chr8.3:25652894-25652913 | MS.gene28514:CDS | 40.0% | |
| CATGTTCAGAAGAGACACTA+TGG | + | chr8.3:25653535-25653554 | MS.gene28514:CDS | 40.0% | |
| GAGCAACTAACATTGTCTAC+CGG | + | chr8.3:25653685-25653704 | MS.gene28514:CDS | 40.0% | |
| GGAAGAATCTCAATGCTTAG+AGG | + | chr8.3:25654095-25654114 | MS.gene28514:CDS | 40.0% | |
| TCGAAAGCTTAAATTCGGCA+GGG | - | chr8.3:25653657-25653676 | None:intergenic | 40.0% | |
| TGTACATCTGGAAAGTCCAT+TGG | + | chr8.3:25653198-25653217 | MS.gene28514:CDS | 40.0% | |
| ! | GACAGTTCATCTGTAATGGA+AGG | + | chr8.3:25652833-25652852 | MS.gene28514:CDS | 40.0% |
| ! | TCATCATTGACGATTGACTC+TGG | - | chr8.3:25653232-25653251 | None:intergenic | 40.0% |
| !! | AAGAAGCATCAAATAGGACC+AGG | + | chr8.3:25653980-25653999 | MS.gene28514:CDS | 40.0% |
| !! | AGACTTGGTGTTTGTACATC+TGG | + | chr8.3:25653186-25653205 | MS.gene28514:CDS | 40.0% |
| !! | GATTGCTCTGAGACAAATCA+AGG | + | chr8.3:25652728-25652747 | MS.gene28514:CDS | 40.0% |
| !! | TGAGAGTGCTGTTAAGTGTA+AGG | + | chr8.3:25653369-25653388 | MS.gene28514:CDS | 40.0% |
| AACACTTGGCAACCATTGGA+TGG | + | chr8.3:25653420-25653439 | MS.gene28514:CDS | 45.0% | |
| AAGAGACACTATGGGCAGTT+TGG | + | chr8.3:25653544-25653563 | MS.gene28514:CDS | 45.0% | |
| ACATCTGCACAAAGCTGAGA+TGG | + | chr8.3:25653081-25653100 | MS.gene28514:CDS | 45.0% | |
| ACCAAACACTTGGCAACCAT+TGG | + | chr8.3:25653416-25653435 | MS.gene28514:CDS | 45.0% | |
| ACCAGAAAGGCTCTATCTGA+AGG | + | chr8.3:25654144-25654163 | MS.gene28514:CDS | 45.0% | |
| AGCTTTCCGGTAATGATCAG+TGG | + | chr8.3:25653293-25653312 | MS.gene28514:CDS | 45.0% | |
| AGTCTTTGCAAATCAGGCTC+TGG | - | chr8.3:25653171-25653190 | None:intergenic | 45.0% | |
| CATCAAATAGGACCAGGAGA+AGG | + | chr8.3:25653986-25654005 | MS.gene28514:CDS | 45.0% | |
| CATCTGCACAAAGCTGAGAT+GGG | + | chr8.3:25653082-25653101 | MS.gene28514:CDS | 45.0% | |
| CCAATTTATCTGCATGAGGC+TGG | - | chr8.3:25654077-25654096 | None:intergenic | 45.0% | |
| CCAGCCTCATGCAGATAAAT+TGG | + | chr8.3:25654074-25654093 | MS.gene28514:CDS | 45.0% | |
| CCATATGAGCAGCTTGATGA+TGG | - | chr8.3:25653849-25653868 | None:intergenic | 45.0% | |
| CCATCATCAAGCTGCTCATA+TGG | + | chr8.3:25653846-25653865 | MS.gene28514:CDS | 45.0% | |
| GACTCTGGATTGATCTCCAA+TGG | - | chr8.3:25653217-25653236 | None:intergenic | 45.0% | |
| GCAGTTGTACCTCCACAAAA+TGG | - | chr8.3:25653007-25653026 | None:intergenic | 45.0% | |
| GTCTTTGCAAATCAGGCTCT+GGG | - | chr8.3:25653170-25653189 | None:intergenic | 45.0% | |
| TCCAATGGTTGCCAAGTGTT+TGG | - | chr8.3:25653420-25653439 | None:intergenic | 45.0% | |
| TTTATCTGCATGAGGCTGGA+TGG | - | chr8.3:25654073-25654092 | None:intergenic | 45.0% | |
| ! | CTTGACATTGTCGACCCTTT+TGG | - | chr8.3:25652874-25652893 | None:intergenic | 45.0% |
| ! | GAGCCTGATTTGCAAAGACT+TGG | + | chr8.3:25653171-25653190 | MS.gene28514:CDS | 45.0% |
| ! | GCAATCACTCCATTTTGTGG+AGG | + | chr8.3:25652995-25653014 | MS.gene28514:CDS | 45.0% |
| ! | GTCATGAAAAGGAAGCACAG+TGG | + | chr8.3:25653446-25653465 | MS.gene28514:CDS | 45.0% |
| !! | ATTAGCTTTAGCCCTTCTCC+TGG | - | chr8.3:25654001-25654020 | None:intergenic | 45.0% |
| CTGAGAATCGAAGTGGTTCG+CGG | + | chr8.3:25653767-25653786 | MS.gene28514:CDS | 50.0% | |
| GAAGCACAGTGGTTGTTCAG+AGG | + | chr8.3:25653457-25653476 | MS.gene28514:CDS | 50.0% | |
| GCCTGTCCACTGATCATTAC+CGG | - | chr8.3:25653302-25653321 | None:intergenic | 50.0% | |
| GCCTTCAGATAGAGCCTTTC+TGG | - | chr8.3:25654148-25654167 | None:intergenic | 50.0% | |
| GTTCGCGGAAGTTTAGTTGC+AGG | + | chr8.3:25653782-25653801 | MS.gene28514:CDS | 50.0% | |
| TCCGGTAATGATCAGTGGAC+AGG | + | chr8.3:25653298-25653317 | MS.gene28514:CDS | 50.0% | |
| CAGTGCGTGAGAACCGCAAT+TGG | + | chr8.3:25652917-25652936 | MS.gene28514:CDS | 55.0% | |
| GGCGAAACGCTCAAGCTTTC+CGG | + | chr8.3:25653280-25653299 | MS.gene28514:CDS | 55.0% | |
| TGGACAGGCTCTCCTAGCTA+TGG | + | chr8.3:25653313-25653332 | MS.gene28514:CDS | 55.0% | |
| ! | TGTGGAGGTACAACTGCTGC+TGG | + | chr8.3:25653010-25653029 | MS.gene28514:CDS | 55.0% |
| !! | TGATGATGGCGACCGAGATC+AGG | - | chr8.3:25653835-25653854 | None:intergenic | 55.0% |
| ACTCTTTGCCAAGCGCCTGC+TGG | - | chr8.3:25653884-25653903 | None:intergenic | 60.0% | |
| AGCAGGCGCTTGGCAAAGAG+TGG | + | chr8.3:25653883-25653902 | MS.gene28514:CDS | 60.0% | |
| CGATCTAGCCAGCAGGCGCT+TGG | + | chr8.3:25653873-25653892 | MS.gene28514:CDS | 65.0% | |
| TGGAGCGCGATCTAGCCAGC+AGG | + | chr8.3:25653866-25653885 | MS.gene28514:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 25652710 | 25654185 | 25652710 | ID=MS.gene28514 |
| chr8.3 | mRNA | 25652710 | 25654185 | 25652710 | ID=MS.gene28514.t1;Parent=MS.gene28514 |
| chr8.3 | exon | 25652710 | 25654185 | 25652710 | ID=MS.gene28514.t1.exon1;Parent=MS.gene28514.t1 |
| chr8.3 | CDS | 25652710 | 25654185 | 25652710 | ID=cds.MS.gene28514.t1;Parent=MS.gene28514.t1 |
| Gene Sequence |
| Protein sequence |
