AlfalfaGEDB | MS.gene28658

M. sativa cultivar XinJiangDaYe / MS.gene28658

KEGG: K12456
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28658.t1	XP_003626105.1	98.4	63	1	0	1	63	1	63	2.20E-26	127.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28658.t1	Q8L981	71.7	60	17	0	1	60	1	60	2.9e-19	95.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28658.t1	Q2HV02	98.4	63	1	0	1	63	1	63	1.6e-26	127.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene28658	0.848166	7.29E-60	-1.69E-46
MS.gene049255	MS.gene28658	0.827888	1.22E-54	-1.69E-46
MS.gene049341	MS.gene28658	0.827971	1.16E-54	-1.69E-46
MS.gene049501	MS.gene28658	0.807547	4.79E-50	-1.69E-46
MS.gene049756	MS.gene28658	0.807299	5.41E-50	-1.69E-46
MS.gene049821	MS.gene28658	0.810769	9.78E-51	-1.69E-46
MS.gene049979	MS.gene28658	0.806875	6.65E-50	-1.69E-46
MS.gene050020	MS.gene28658	0.860633	1.80E-63	-1.69E-46
MS.gene050303	MS.gene28658	0.815568	8.65E-52	-1.69E-46
MS.gene050438	MS.gene28658	0.813986	1.94E-51	-1.69E-46
MS.gene050442	MS.gene28658	0.809708	1.66E-50	-1.69E-46
MS.gene050586	MS.gene28658	0.809871	1.53E-50	-1.69E-46
MS.gene050719	MS.gene28658	0.811603	6.44E-51	-1.69E-46
MS.gene050721	MS.gene28658	0.812205	4.77E-51	-1.69E-46
MS.gene050724	MS.gene28658	0.829247	5.73E-55	-1.69E-46
MS.gene050807	MS.gene28658	0.841606	4.29E-58	-1.69E-46
MS.gene050808	MS.gene28658	0.801358	9.35E-49	-1.69E-46
MS.gene050815	MS.gene28658	0.991722	3.51E-189	-1.69E-46
MS.gene050844	MS.gene28658	0.802595	5.21E-49	-1.69E-46
MS.gene051180	MS.gene28658	0.809631	1.72E-50	-1.69E-46
MS.gene051243	MS.gene28658	0.851449	8.81E-61	-1.69E-46
MS.gene051245	MS.gene28658	0.803108	4.08E-49	-1.69E-46
MS.gene051247	MS.gene28658	0.806063	9.87E-50	-1.69E-46
MS.gene051328	MS.gene28658	0.845501	3.90E-59	-1.69E-46
MS.gene05134	MS.gene28658	0.81704	4.05E-52	-1.69E-46
MS.gene051719	MS.gene28658	0.813272	2.78E-51	-1.69E-46
MS.gene051720	MS.gene28658	0.808622	2.83E-50	-1.69E-46
MS.gene051757	MS.gene28658	0.802621	5.14E-49	-1.69E-46
MS.gene051789	MS.gene28658	0.814363	1.60E-51	-1.69E-46
MS.gene052012	MS.gene28658	0.837718	4.41E-57	-1.69E-46
MS.gene052323	MS.gene28658	0.802247	6.14E-49	-1.69E-46
MS.gene052519	MS.gene28658	0.80289	4.53E-49	-1.69E-46
MS.gene052613	MS.gene28658	0.801192	1.01E-48	-1.69E-46
MS.gene052665	MS.gene28658	0.834169	3.50E-56	-1.69E-46
MS.gene052863	MS.gene28658	0.841591	4.33E-58	-1.69E-46
MS.gene05288	MS.gene28658	0.801964	7.02E-49	-1.69E-46
MS.gene052896	MS.gene28658	0.85498	8.58E-62	-1.69E-46
MS.gene053008	MS.gene28658	0.876784	1.06E-68	-1.69E-46
MS.gene053074	MS.gene28658	0.837892	3.98E-57	-1.69E-46
MS.gene053221	MS.gene28658	0.801348	9.40E-49	-1.69E-46
MS.gene054184	MS.gene28658	0.802916	4.47E-49	-1.69E-46
MS.gene054185	MS.gene28658	0.807001	6.26E-50	-1.69E-46
MS.gene054433	MS.gene28658	0.800512	1.39E-48	-1.69E-46
MS.gene054472	MS.gene28658	0.832225	1.07E-55	-1.69E-46
MS.gene054658	MS.gene28658	0.810221	1.28E-50	-1.69E-46
MS.gene054823	MS.gene28658	0.802364	5.81E-49	-1.69E-46
MS.gene05510	MS.gene28658	0.807361	5.25E-50	-1.69E-46
MS.gene055162	MS.gene28658	0.816006	6.91E-52	-1.69E-46
MS.gene055465	MS.gene28658	0.813267	2.79E-51	-1.69E-46
MS.gene055466	MS.gene28658	0.851931	6.44E-61	-1.69E-46
MS.gene055468	MS.gene28658	0.845018	5.27E-59	-1.69E-46
MS.gene055512	MS.gene28658	0.813218	2.86E-51	-1.69E-46
MS.gene055608	MS.gene28658	0.826314	2.90E-54	-1.69E-46
MS.gene055623	MS.gene28658	0.807173	5.75E-50	-1.69E-46
MS.gene055818	MS.gene28658	0.83215	1.11E-55	-1.69E-46
MS.gene056069	MS.gene28658	-0.833937	4.00E-56	-1.69E-46
MS.gene056631	MS.gene28658	0.804059	2.59E-49	-1.69E-46
MS.gene056760	MS.gene28658	0.830428	2.95E-55	-1.69E-46
MS.gene057057	MS.gene28658	0.809798	1.58E-50	-1.69E-46
MS.gene057060	MS.gene28658	0.811625	6.38E-51	-1.69E-46
MS.gene057140	MS.gene28658	0.831374	1.73E-55	-1.69E-46
MS.gene057260	MS.gene28658	0.801306	9.58E-49	-1.69E-46
MS.gene057693	MS.gene28658	0.820867	5.47E-53	-1.69E-46
MS.gene058084	MS.gene28658	0.83638	9.68E-57	-1.69E-46
MS.gene058146	MS.gene28658	0.809477	1.86E-50	-1.69E-46
MS.gene058168	MS.gene28658	0.827943	1.18E-54	-1.69E-46
MS.gene058636	MS.gene28658	0.808729	2.68E-50	-1.69E-46
MS.gene058646	MS.gene28658	0.815536	8.79E-52	-1.69E-46
MS.gene058906	MS.gene28658	0.85272	3.83E-61	-1.69E-46
MS.gene059006	MS.gene28658	0.80892	2.44E-50	-1.69E-46
MS.gene059151	MS.gene28658	0.816289	5.97E-52	-1.69E-46
MS.gene059842	MS.gene28658	0.835626	1.50E-56	-1.69E-46
MS.gene059937	MS.gene28658	0.836431	9.40E-57	-1.69E-46
MS.gene060137	MS.gene28658	-0.805745	1.15E-49	-1.69E-46
MS.gene060322	MS.gene28658	0.847755	9.46E-60	-1.69E-46
MS.gene060429	MS.gene28658	0.826022	3.41E-54	-1.69E-46
MS.gene060683	MS.gene28658	0.810401	1.17E-50	-1.69E-46
MS.gene060685	MS.gene28658	0.812437	4.24E-51	-1.69E-46
MS.gene060802	MS.gene28658	0.828487	8.75E-55	-1.69E-46
MS.gene060803	MS.gene28658	0.874965	4.47E-68	-1.69E-46
MS.gene060998	MS.gene28658	0.806293	8.82E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28658.t1	MTR_7g111090	98.413	63	1	0	1	63	1	63	4.73e-39	123

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28658.t1	AT1G73177	71.667	60	17	0	1	60	1	60	4.22e-16	65.5

Find 17 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGGCACAACAAGTTAATA+AGG	0.370280	7.1:-4033802	MS.gene28658:CDS
TGCCTCCAACACTGGTTGTT+CGG	0.370391	7.1:-4034380	MS.gene28658:CDS
TTTATTGAAACTTCTACTCT+TGG	0.408151	7.1:+4033754	None:intergenic
GCTTGGCACGATCTTGCCCT+TGG	0.435718	7.1:-4033777	MS.gene28658:CDS
AAAATGGCAGAACTGAGTTT+GGG	0.446438	7.1:-4034979	MS.gene28658:CDS
TTGACATTGTTGATGAGGAT+TGG	0.447381	7.1:-4034948	MS.gene28658:CDS
ACAACCAGTGTTGGAGGCAG+TGG	0.463826	7.1:+4034384	None:intergenic
TCTTCCACTGCCTCCAACAC+TGG	0.491395	7.1:-4034388	MS.gene28658:CDS
GAAACTTCTACTCTTGGCCA+AGG	0.510239	7.1:+4033760	None:intergenic
TCTAATTGACATTGTTGATG+AGG	0.512652	7.1:-4034953	MS.gene28658:CDS
AACAAGTTAATAAGGATGCT+TGG	0.520232	7.1:-4033794	MS.gene28658:CDS
GTCCGAACAACCAGTGTTGG+AGG	0.546833	7.1:+4034378	None:intergenic
AGAGACATTCTTCCTCAAGA+TGG	0.570997	7.1:-4034922	MS.gene28658:intron
TCTGTCCGAACAACCAGTGT+TGG	0.577627	7.1:+4034375	None:intergenic
TTGTTGTTAATCAGATCAGG+AGG	0.619606	7.1:-4033820	MS.gene28658:intron
AAACTTCTACTCTTGGCCAA+GGG	0.628656	7.1:+4033761	None:intergenic
TGTTCGGACAGATGATACTG+AGG	0.734610	7.1:-4034364	MS.gene28658:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTTATCAATATACAATAT+AGG	+	chr7.1:4034133-4034152	None:intergenic	10.0%
!!	AAAAAAAAAATACCATCTTG+AGG	+	chr7.1:4033836-4033855	None:intergenic	20.0%
!!	CAAATATAACAAAGTATAGA+TGG	-	chr7.1:4034198-4034217	MS.gene28658:intron	20.0%
!!	TAAAAAACAGTTGCAAAAAA+AGG	-	chr7.1:4033957-4033976	MS.gene28658:intron	20.0%
!!	TTTAAAAGAAAATGTGTTCT+TGG	-	chr7.1:4034616-4034635	MS.gene28658:intron	20.0%
!!!	ACTTCTTATTTCATTTACAA+TGG	+	chr7.1:4034029-4034048	None:intergenic	20.0%
!!!	ATGTTTTCAAACTTATATGT+CGG	-	chr7.1:4034796-4034815	MS.gene28658:intron	20.0%
!	ATCAAAAAGTTGCAATTTAG+TGG	-	chr7.1:4034568-4034587	MS.gene28658:intron	25.0%
!	ATCAATATACAATATAGGAC+AGG	+	chr7.1:4034128-4034147	None:intergenic	25.0%
!	ATTGATTATTGTTCATGAGA+TGG	-	chr7.1:4034842-4034861	MS.gene28658:intron	25.0%
!	GTAAAATTAGTATACACTGA+CGG	+	chr7.1:4033897-4033916	None:intergenic	25.0%
!	TATAACAAAGTATAGATGGT+TGG	-	chr7.1:4034202-4034221	MS.gene28658:intron	25.0%
!	TCAATATACAATATAGGACA+GGG	+	chr7.1:4034127-4034146	None:intergenic	25.0%
!	TCGAATAATCTATTTGTAGT+TGG	+	chr7.1:4033927-4033946	None:intergenic	25.0%
!	TTGATTATTGTTCATGAGAT+GGG	-	chr7.1:4034843-4034862	MS.gene28658:intron	25.0%
!!	CTAAATTGCAACTTTTTGAT+TGG	+	chr7.1:4034568-4034587	None:intergenic	25.0%
!!	TGTTTTCAAACTTATATGTC+GGG	-	chr7.1:4034797-4034816	MS.gene28658:intron	25.0%
	AAAAAGGCTACATAGCAAAA+TGG	-	chr7.1:4033973-4033992	MS.gene28658:intron	30.0%
	AACAAGTTAATAAGGATGCT+TGG	-	chr7.1:4034949-4034968	MS.gene28658:CDS	30.0%
	AACTATATATGTTGCCATGT+TGG	-	chr7.1:4034741-4034760	MS.gene28658:intron	30.0%
	ACGAAGAGAATGTAAGTTTA+GGG	-	chr7.1:4034246-4034265	MS.gene28658:intron	30.0%
	CTGATTAACAACAAATCACA+TGG	+	chr7.1:4034917-4034936	None:intergenic	30.0%
	GAATTGCTTCAATGAAAACA+TGG	-	chr7.1:4034590-4034609	MS.gene28658:intron	30.0%
	GGCTACATAACTTATAATAG+TGG	-	chr7.1:4034819-4034838	MS.gene28658:intron	30.0%
	TAAACTAAGCTCAAGAAAGA+TGG	+	chr7.1:4034455-4034474	None:intergenic	30.0%
	TACGAAGAGAATGTAAGTTT+AGG	-	chr7.1:4034245-4034264	MS.gene28658:intron	30.0%
	TCTAATTGACATTGTTGATG+AGG	-	chr7.1:4033790-4033809	MS.gene28658:CDS	30.0%
!	GTTTTCAAACTTATATGTCG+GGG	-	chr7.1:4034798-4034817	MS.gene28658:intron	30.0%
!	TTGATAATTGATATCCACGT+GGG	-	chr7.1:4034300-4034319	MS.gene28658:intron	30.0%
!!	GATTTGTTGTTAATCAGATC+AGG	-	chr7.1:4034920-4034939	MS.gene28658:intron	30.0%
	AATCTATTTGTAGTTGGCGT+TGG	+	chr7.1:4033921-4033940	None:intergenic	35.0%
	AGAGAATGTAAGTTTAGGGA+TGG	-	chr7.1:4034250-4034269	MS.gene28658:intron	35.0%
	AGTTTAGGGATGGATGAAAT+TGG	-	chr7.1:4034260-4034279	MS.gene28658:intron	35.0%
	ATACAATATAGGACAGGGAT+GGG	+	chr7.1:4034122-4034141	None:intergenic	35.0%
	GACTAAAGCAGTTACATTTG+TGG	-	chr7.1:4034426-4034445	MS.gene28658:intron	35.0%
	GATAAATTGTCCTGAACTGA+CGG	-	chr7.1:4034145-4034164	MS.gene28658:intron	35.0%
	TATACAATATAGGACAGGGA+TGG	+	chr7.1:4034123-4034142	None:intergenic	35.0%
!	CTTGATAATTGATATCCACG+TGG	-	chr7.1:4034299-4034318	MS.gene28658:intron	35.0%
!	GTATTAAGCACACGTTGATT+TGG	-	chr7.1:4034488-4034507	MS.gene28658:intron	35.0%
!	TTGACATTGTTGATGAGGAT+TGG	-	chr7.1:4033795-4033814	MS.gene28658:CDS	35.0%
!	TTGTTGTTAATCAGATCAGG+AGG	-	chr7.1:4034923-4034942	MS.gene28658:CDS	35.0%
	ACAATGCATTACTGACAGTG+TGG	-	chr7.1:4034671-4034690	MS.gene28658:intron	40.0%
	ACAGTGTGGCATGTAAACAA+CGG	-	chr7.1:4034685-4034704	MS.gene28658:intron	40.0%
	AGAGACATTCTTCCTCAAGA+TGG	-	chr7.1:4033821-4033840	MS.gene28658:intron	40.0%
	ATATGTTGCCATGTTGGTGT+CGG	-	chr7.1:4034747-4034766	MS.gene28658:intron	40.0%
	ATCAGATTTACATTCCCACG+TGG	+	chr7.1:4034317-4034336	None:intergenic	40.0%
	CAAATATGTACCGTCAGTTC+AGG	+	chr7.1:4034158-4034177	None:intergenic	40.0%
	TATGCACGCTGTTGTCTAAT+TGG	+	chr7.1:4034005-4034024	None:intergenic	40.0%
!	GGAGGCACAACAAGTTAATA+AGG	-	chr7.1:4034941-4034960	MS.gene28658:CDS	40.0%
!	TCATGAGATGGGTCTTATGT+TGG	-	chr7.1:4034854-4034873	MS.gene28658:intron	40.0%
!!	CACACGTTGATTTGGTTGAT+GGG	-	chr7.1:4034496-4034515	MS.gene28658:intron	40.0%
	TGTCGGATACAGACACATGT+TGG	-	chr7.1:4034764-4034783	MS.gene28658:intron	45.0%
	TGTTCGGACAGATGATACTG+AGG	-	chr7.1:4034379-4034398	MS.gene28658:CDS	45.0%
!	TGAGATGGGTCTTATGTTGG+AGG	-	chr7.1:4034857-4034876	MS.gene28658:intron	45.0%
!!	GCACACGTTGATTTGGTTGA+TGG	-	chr7.1:4034495-4034514	MS.gene28658:intron	45.0%
	GTCTGTATCCGACACCAACA+TGG	+	chr7.1:4034758-4034777	None:intergenic	50.0%
	TCTGTCCGAACAACCAGTGT+TGG	+	chr7.1:4034371-4034390	None:intergenic	50.0%
!	TGCCTCCAACACTGGTTGTT+CGG	-	chr7.1:4034363-4034382	MS.gene28658:CDS	50.0%
	TCTTCCACTGCCTCCAACAC+TGG	-	chr7.1:4034355-4034374	MS.gene28658:intron	55.0%
!	ACAACCAGTGTTGGAGGCAG+TGG	+	chr7.1:4034362-4034381	None:intergenic	55.0%
!	GTCCGAACAACCAGTGTTGG+AGG	+	chr7.1:4034368-4034387	None:intergenic	55.0%
	GCTTGGCACGATCTTGCCCT+TGG	-	chr7.1:4034966-4034985	MS.gene28658:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	4033767	4034998	4033767	ID=MS.gene28658
chr7.1	mRNA	4033767	4034998	4033767	ID=MS.gene28658.t1;Parent=MS.gene28658
chr7.1	exon	4034923	4034998	4034923	ID=MS.gene28658.t1.exon1;Parent=MS.gene28658.t1
chr7.1	CDS	4034923	4034998	4034923	ID=cds.MS.gene28658.t1;Parent=MS.gene28658.t1
chr7.1	exon	4034358	4034411	4034358	ID=MS.gene28658.t1.exon2;Parent=MS.gene28658.t1
chr7.1	CDS	4034358	4034411	4034358	ID=cds.MS.gene28658.t1;Parent=MS.gene28658.t1
chr7.1	exon	4033767	4033828	4033767	ID=MS.gene28658.t1.exon3;Parent=MS.gene28658.t1
chr7.1	CDS	4033767	4033828	4033767	ID=cds.MS.gene28658.t1;Parent=MS.gene28658.t1

Gene Sequence

>MS.gene28658

ATGGCAGAACTGAGTTTGGGAATTCTAATTGACATTGTTGATGAGGATTGGATGAGAGACATTCTTCCTCAAGATGGTATTTTTTTTTTTCTTCTTTAATGCATTTTGTTGAGAAAAAAGATATGCGCCGTCAGTGTATACTAATTTTACACCAACGCCAACTACAAATAGATTATTCGACAGTTATCTTTAAAAAACAGTTGCAAAAAAAGGCTACATAGCAAAATGGTTAGTTCCAATTAGACAACAGCGTGCATAACCATTGTAAATGAAATAAGAAGTTGAATCATCTATTGAGAGTCTTAAGCAGTCTTACACATTCTGTTTATGTAGTCTTAAACATTTTGTTACACCCATCCCTGTCCTATATTGTATATTGATAAATTGTCCTGAACTGACGGTACATATTTGAAAATACGTGAATAAATGAACAAATATAACAAAGTATAGATGGTTGGTTTACTTGTGATCTACAAAGTACGAAGAGAATGTAAGTTTAGGGATGGATGAAATTGGTGATATTTTCTTTTAACTTGATAATTGATATCCACGTGGGAATGTAAATCTGATGTGTGCTGTTTTTACAGATCTTCCACTGCCTCCAACACTGGTTGTTCGGACAGATGATACTGAGGACTCAAGTATGTTCTTAATTCATTGACTAAAGCAGTTACATTTGTGGATACCATCTTTCTTGAGCTTAGTTTATTAAAACATTGTTGTATTAAGCACACGTTGATTTGGTTGATGGGAAGATGTATATTAAAAATGAATGAAAAAGATGTATACACGAGAAAACCAATCAAAAAGTTGCAATTTAGTGGAATTGCTTCAATGAAAACATGGTTGTTTAAAAGAAAATGTGTTCTTGGACATGCAACTGCACTCTTACAATTTATGATGCACAATGCATTACTGACAGTGTGGCATGTAAACAACGGTCATAACTTAAGAAAATTGAAGTGATTGATTGTAACTATATATGTTGCCATGTTGGTGTCGGATACAGACACATGTTGGACACTGAACATGTTTTCAAACTTATATGTCGGGGCTACATAACTTATAATAGTGGATTGATTATTGTTCATGAGATGGGTCTTATGTTGGAGGATTGTTTCTGCTTTTTGTTGTTGACTTTTTAACACCATGTGATTTGTTGTTAATCAGATCAGGAGGCACAACAAGTTAATAAGGATGCTTGGCACGATCTTGCCCTTGGCCAAGAGTAG

Protein sequence

>MS.gene28658.t1

MAELSLGILIDIVDEDWMRDILPQDDLPLPPTLVVRTDDTEDSNQEAQQVNKDAWHDLALGQE