AlfalfaGEDB | MS.gene57158

M. sativa cultivar XinJiangDaYe / MS.gene57158

KEGG: K02728
GO: GO:0003674 GO:0003824 GO:0004175 GO:0004298 GO:0005575 GO:0005839 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0019538 GO:0019773 GO:0019941 GO:0032991 GO:0043170 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0051603 GO:0070003 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901575
PFAM: PF00227.29 PF10584.12

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57158.t1	XP_003611674.1	100	250	0	0	1	250	1	250	1.10E-134	489.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57158.t1	P52427	90.0	250	25	0	1	250	1	250	8.3e-126	451.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57158.t1	G7K595	100.0	250	0	0	1	250	1	250	8.3e-135	489.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene006641	MS.gene57158	PPI
MS.gene045566	MS.gene57158	PPI
MS.gene012131	MS.gene57158	PPI
MS.gene92186	MS.gene57158	PPI
MS.gene071304	MS.gene57158	PPI
MS.gene074061	MS.gene57158	PPI
MS.gene27289	MS.gene57158	PPI
MS.gene44226	MS.gene57158	PPI
MS.gene47175	MS.gene57158	PPI
MS.gene77139	MS.gene57158	PPI
MS.gene24252	MS.gene57158	PPI
MS.gene058061	MS.gene57158	PPI
MS.gene57158	MS.gene27289	PPI
MS.gene74962	MS.gene57158	PPI
MS.gene074000	MS.gene57158	PPI
MS.gene071345	MS.gene57158	PPI
MS.gene57158	MS.gene76217	PPI
MS.gene046093	MS.gene57158	PPI
MS.gene066737	MS.gene57158	PPI
MS.gene21169	MS.gene57158	PPI
MS.gene63845	MS.gene57158	PPI
MS.gene24255	MS.gene57158	PPI
MS.gene89677	MS.gene57158	PPI
MS.gene09974	MS.gene57158	PPI
MS.gene27247	MS.gene57158	PPI
MS.gene017616	MS.gene57158	PPI
MS.gene029314	MS.gene57158	PPI
MS.gene039040	MS.gene57158	PPI
MS.gene24253	MS.gene57158	PPI
MS.gene74417	MS.gene57158	PPI
MS.gene024828	MS.gene57158	PPI
MS.gene26985	MS.gene57158	PPI
MS.gene025808	MS.gene57158	PPI
MS.gene29922	MS.gene57158	PPI
MS.gene066738	MS.gene57158	PPI
MS.gene21801	MS.gene57158	PPI
MS.gene48062	MS.gene57158	PPI
MS.gene84459	MS.gene57158	PPI
MS.gene64609	MS.gene57158	PPI
MS.gene55530	MS.gene57158	PPI
MS.gene90698	MS.gene57158	PPI
MS.gene043801	MS.gene57158	PPI
MS.gene29256	MS.gene57158	PPI
MS.gene065045	MS.gene57158	PPI
MS.gene40486	MS.gene57158	PPI
MS.gene070615	MS.gene57158	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57158.t1	MTR_5g016590	100.000	250	0	0	1	250	1	250	0.0	518
MS.gene57158.t1	MTR_7g070070	98.000	250	5	0	1	250	1	250	0.0	509
MS.gene57158.t1	MTR_1g112250	42.324	241	121	7	5	239	8	236	6.23e-59	187
MS.gene57158.t1	MTR_8g075320	42.500	200	112	3	4	202	3	200	4.37e-53	172
MS.gene57158.t1	MTR_2g016050	41.500	200	114	3	4	202	3	200	9.18e-51	166
MS.gene57158.t1	MTR_0167s0060	36.325	234	142	4	4	237	5	231	4.41e-48	159
MS.gene57158.t1	MTR_1g022410	38.785	214	125	3	4	215	5	214	3.24e-43	148
MS.gene57158.t1	MTR_8g464460	41.709	199	112	3	5	200	9	206	3.24e-43	147
MS.gene57158.t1	MTR_2g060830	36.975	238	138	6	5	237	9	239	1.19e-40	140
MS.gene57158.t1	MTR_6g024135	35.071	211	135	2	5	215	8	216	2.11e-37	132
MS.gene57158.t1	MTR_4g012740	28.968	252	135	6	4	215	236	483	6.44e-22	94.7
MS.gene57158.t1	MTR_6g013300	32.821	195	112	8	33	220	138	320	1.09e-16	78.6
MS.gene57158.t1	MTR_4g057090	29.947	187	119	5	26	207	110	289	3.14e-14	71.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57158.t1	AT3G22110	90.400	250	24	0	1	250	1	250	3.64e-173	477
MS.gene57158.t1	AT4G15165	72.000	250	28	1	1	250	1	208	3.76e-127	359
MS.gene57158.t1	AT3G14290	42.149	242	122	7	4	239	7	236	1.83e-59	188
MS.gene57158.t1	AT1G53850	41.322	242	124	7	4	239	7	236	1.17e-58	186
MS.gene57158.t1	AT1G53850	41.322	242	124	7	4	239	7	236	1.17e-58	186
MS.gene57158.t1	AT5G66140	42.000	200	113	3	4	202	3	200	1.78e-52	171
MS.gene57158.t1	AT3G51260	42.500	200	112	3	4	202	3	200	2.12e-52	171
MS.gene57158.t1	AT3G51260	42.500	200	112	3	4	202	3	200	2.31e-52	170
MS.gene57158.t1	AT1G16470	37.264	212	130	3	4	215	5	213	1.32e-46	155
MS.gene57158.t1	AT1G16470	37.264	212	130	3	4	215	5	213	1.32e-46	155
MS.gene57158.t1	AT1G79210	37.264	212	130	3	4	215	5	213	1.56e-46	155
MS.gene57158.t1	AT1G79210	37.264	212	130	3	4	215	5	213	1.56e-46	155
MS.gene57158.t1	AT1G79210	37.264	212	130	3	4	215	5	213	1.56e-46	155
MS.gene57158.t1	AT5G42790	39.320	206	119	3	4	207	5	206	5.65e-44	150
MS.gene57158.t1	AT1G47250	38.350	206	121	3	4	207	5	206	3.06e-43	148
MS.gene57158.t1	AT5G35590	40.201	199	115	3	5	200	9	206	2.10e-41	142
MS.gene57158.t1	AT2G05840	37.688	199	120	3	5	200	9	206	5.22e-38	132
MS.gene57158.t1	AT2G05840	37.688	199	120	3	5	200	9	206	5.93e-38	133
MS.gene57158.t1	AT2G05840	37.745	204	116	4	5	205	9	204	3.34e-37	130
MS.gene57158.t1	AT2G27020	32.314	229	150	4	5	233	8	231	2.77e-36	129
MS.gene57158.t1	AT2G27020	32.314	229	150	4	5	233	110	333	3.24e-35	129

Find 73 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGTCTCTTTCCTGTTTGC+AGG	0.211747	5.2:+8295679	MS.gene57158:CDS
CTCTTTCCTGTTTGCAGGAT+GGG	0.248276	5.2:+8295684	MS.gene57158:CDS
ACTAAGCTGTTGGTGAAGTC+TGG	0.280754	5.2:+8295988	MS.gene57158:CDS
CATGTTGCATGTGCTGTTGC+TGG	0.302104	5.2:+8295499	MS.gene57158:CDS
AAGCAAGGTTACACACAATT+TGG	0.305332	5.2:+8295640	MS.gene57158:CDS
GGATCCTAGCAGTGTTGATT+AGG	0.325400	5.2:-8295543	None:intergenic
GTCGAAGGATGGGGTTGTTC+TGG	0.338789	5.2:+8295402	MS.gene57158:CDS
AGCAGTCTCTGTTGCTGGTT+GGG	0.364107	5.2:-8296016	None:intergenic
AGCGATCCTAGTGGAAATTA+TGG	0.367986	5.2:+8295736	MS.gene57158:CDS
GAAAGTTCTGAGCAAAACTA+TGG	0.379385	5.2:+8295867	MS.gene57158:CDS
AACAGCTTAGTCAAGTTCTC+TGG	0.381164	5.2:-8295976	None:intergenic
AGCTAAGCAGTCTCTGTTGC+TGG	0.412030	5.2:-8296021	None:intergenic
TTTGGTGGTCTCCGTCCGTT+TGG	0.423167	5.2:+8295658	MS.gene57158:CDS
GGTTGGAAAGCCGGTGCTAT+TGG	0.431124	5.2:+8295760	MS.gene57158:CDS
TGATTGTGCTGCCTGGTTGT+TGG	0.432231	5.2:-8295785	None:intergenic
AGCACAATCAATTCTAAAAC+AGG	0.435611	5.2:+8295798	MS.gene57158:CDS
GATTGAACCAACTGTTCAAC+AGG	0.438880	5.2:-8295604	None:intergenic
GTTCAACAGGCATTGGCTCT+TGG	0.441840	5.2:-8295591	None:intergenic
CTAAGCTGTTGGTGAAGTCT+GGG	0.453484	5.2:+8295989	MS.gene57158:CDS
TCTCTTTCCTGTTTGCAGGA+TGG	0.460327	5.2:+8295683	MS.gene57158:CDS
AAGCAGTCTCTGTTGCTGGT+TGG	0.471981	5.2:-8296017	None:intergenic
ATGTTGCATGTGCTGTTGCT+GGG	0.490338	5.2:+8295500	MS.gene57158:CDS
TGTTCTGGTTGGCGAAAAGA+AGG	0.492197	5.2:+8295417	MS.gene57158:CDS
AATTATGGCGGTTGGAAAGC+CGG	0.495351	5.2:+8295751	MS.gene57158:CDS
TGCTCAGAACTTTCAGTGCA+AGG	0.496408	5.2:-8295858	None:intergenic
ACAGCTTAGTCAAGTTCTCT+GGG	0.496829	5.2:-8295975	None:intergenic
AGCAGTGTTGATTAGGATGT+TGG	0.496912	5.2:-8295536	None:intergenic
GCCAATGCCTGTTGAACAGT+TGG	0.499789	5.2:+8295597	MS.gene57158:CDS
TTAGAATTGATTGTGCTGCC+TGG	0.504956	5.2:-8295792	None:intergenic
AAGCTTATCCGAGGTAAGAC+TGG	0.507716	5.2:-8295896	None:intergenic
TGTTGAGGTTTGCAGAAGCT+TGG	0.509366	5.2:-8295446	None:intergenic
ACTTGGTAAAGACGTCCTTC+AGG	0.509793	5.2:-8295328	None:intergenic
TAGGAATCTTGTCGAAGGAT+GGG	0.512721	5.2:+8295392	MS.gene57158:CDS
CAAATGAGTAACGTTGTGCT+TGG	0.513670	5.2:-8295564	None:intergenic
CAACCGCCATAATTTCCACT+AGG	0.518150	5.2:-8295742	None:intergenic
AAGGATGGGGTTGTTCTGGT+TGG	0.521102	5.2:+8295406	MS.gene57158:CDS
ACCAACTGTTCAACAGGCAT+TGG	0.523493	5.2:-8295598	None:intergenic
GGAAATGCTGGTACTGCCAT+AGG	0.528749	5.2:+8295373	MS.gene57158:CDS
CTAGTGGAAATTATGGCGGT+TGG	0.531106	5.2:+8295743	MS.gene57158:CDS
ATAGGAATCTTGTCGAAGGA+TGG	0.537221	5.2:+8295391	MS.gene57158:CDS
TGCTATTGGTGCCAACAACC+AGG	0.540083	5.2:+8295774	MS.gene57158:CDS
ATCCTTCGACAAGATTCCTA+TGG	0.544761	5.2:-8295389	None:intergenic
ATGGAGGCCATTGGAAATGC+TGG	0.546199	5.2:+8295361	MS.gene57158:CDS
AAATTGTGTGTAACCTTGCT+TGG	0.550671	5.2:-8295638	None:intergenic
CCTCCATTGCATACTCCACT+TGG	0.550938	5.2:-8295345	None:intergenic
AATTCTAAAACAGGACTACA+AGG	0.553479	5.2:+8295807	MS.gene57158:CDS
AGGAATCTTGTCGAAGGATG+GGG	0.558136	5.2:+8295393	MS.gene57158:CDS
ACATCCTAATCAACACTGCT+AGG	0.565540	5.2:+8295539	MS.gene57158:CDS
CAAGGTTACACACAATTTGG+TGG	0.565666	5.2:+8295643	MS.gene57158:CDS
GTTGTTGGCACCAATAGCAC+CGG	0.570780	5.2:-8295770	None:intergenic
GGATAAGCTTGAACTTGCAG+AGG	0.575420	5.2:+8295909	MS.gene57158:CDS
GTTGATTAGGATGTTGGCAT+CGG	0.579727	5.2:-8295530	None:intergenic
AACAGGAAAGAGACTCCAAA+CGG	0.582222	5.2:-8295673	None:intergenic
CAGGAGAGAAGATTGTTGTA+CGG	0.584834	5.2:-8295309	None:intergenic
CCAAGTGGAGTATGCAATGG+AGG	0.586792	5.2:+8295345	MS.gene57158:CDS
GAGTATGCAATGGAGGCCAT+TGG	0.587807	5.2:+8295352	MS.gene57158:CDS
AGAGAACTTGACTAAGCTGT+TGG	0.594383	5.2:+8295978	MS.gene57158:CDS
ACAACAATCTTCTCTCCTGA+AGG	0.598896	5.2:+8295313	MS.gene57158:CDS
TTACCAAGTGGAGTATGCAA+TGG	0.599728	5.2:+8295342	MS.gene57158:CDS
TCTCTTTGTGATACCAAGCA+AGG	0.600349	5.2:+8295625	MS.gene57158:CDS
GGAAAGAGACTCCAAACGGA+CGG	0.606946	5.2:-8295669	None:intergenic
TGCCATAGGAATCTTGTCGA+AGG	0.620793	5.2:+8295387	MS.gene57158:CDS
GGCAGTACCAGCATTTCCAA+TGG	0.624766	5.2:-8295368	None:intergenic
TGATACTTGACCTTTCCAGA+TGG	0.634603	5.2:-8295943	None:intergenic
TGCAAGTTCAAGCTTATCCG+AGG	0.657193	5.2:-8295905	None:intergenic
TGAAGGACGTCTTTACCAAG+TGG	0.670157	5.2:+8295330	MS.gene57158:CDS
CTTTACATGAGCGATCCTAG+TGG	0.672737	5.2:+8295727	MS.gene57158:CDS
GGACAGCACCAGTCTTACCT+CGG	0.676388	5.2:+8295888	MS.gene57158:CDS
TAAGCTGTTGGTGAAGTCTG+GGG	0.680201	5.2:+8295990	MS.gene57158:CDS
ACTACAAGGATGATATCACA+AGG	0.683851	5.2:+8295821	MS.gene57158:CDS
GATCCTAGTGGAAATTATGG+CGG	0.717061	5.2:+8295739	MS.gene57158:CDS
CAGCTTAGTCAAGTTCTCTG+GGG	0.728921	5.2:-8295974	None:intergenic
CTACAAGGATGATATCACAA+GGG	0.766354	5.2:+8295822	MS.gene57158:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTCTAAAACAGGACTACA+AGG	+	chr5.2:8295807-8295826	MS.gene57158:CDS	30.0%
!!	AGCACAATCAATTCTAAAAC+AGG	+	chr5.2:8295798-8295817	MS.gene57158:CDS	30.0%
	AAATTGTGTGTAACCTTGCT+TGG	-	chr5.2:8295641-8295660	None:intergenic	35.0%
	AAGCAAGGTTACACACAATT+TGG	+	chr5.2:8295640-8295659	MS.gene57158:CDS	35.0%
	ACTACAAGGATGATATCACA+AGG	+	chr5.2:8295821-8295840	MS.gene57158:CDS	35.0%
	CTACAAGGATGATATCACAA+GGG	+	chr5.2:8295822-8295841	MS.gene57158:CDS	35.0%
!!	CATTTTCTCAGTTGATGTTG+AGG	-	chr5.2:8295464-8295483	None:intergenic	35.0%
!!	GAAAGTTCTGAGCAAAACTA+TGG	+	chr5.2:8295867-8295886	MS.gene57158:CDS	35.0%
	AACAGCTTAGTCAAGTTCTC+TGG	-	chr5.2:8295979-8295998	None:intergenic	40.0%
	AACAGGAAAGAGACTCCAAA+CGG	-	chr5.2:8295676-8295695	None:intergenic	40.0%
	ACAACAATCTTCTCTCCTGA+AGG	+	chr5.2:8295313-8295332	MS.gene57158:CDS	40.0%
	ACAGCTTAGTCAAGTTCTCT+GGG	-	chr5.2:8295978-8295997	None:intergenic	40.0%
	ACATCCTAATCAACACTGCT+AGG	+	chr5.2:8295539-8295558	MS.gene57158:CDS	40.0%
	AGAGAACTTGACTAAGCTGT+TGG	+	chr5.2:8295978-8295997	MS.gene57158:CDS	40.0%
	AGCGATCCTAGTGGAAATTA+TGG	+	chr5.2:8295736-8295755	MS.gene57158:CDS	40.0%
	ATAGGAATCTTGTCGAAGGA+TGG	+	chr5.2:8295391-8295410	MS.gene57158:CDS	40.0%
	ATCCTTCGACAAGATTCCTA+TGG	-	chr5.2:8295392-8295411	None:intergenic	40.0%
	CAAATGAGTAACGTTGTGCT+TGG	-	chr5.2:8295567-8295586	None:intergenic	40.0%
	CAAGGTTACACACAATTTGG+TGG	+	chr5.2:8295643-8295662	MS.gene57158:CDS	40.0%
	CAGGAGAGAAGATTGTTGTA+CGG	-	chr5.2:8295312-8295331	None:intergenic	40.0%
	GATCCTAGTGGAAATTATGG+CGG	+	chr5.2:8295739-8295758	MS.gene57158:CDS	40.0%
	GATTGAACCAACTGTTCAAC+AGG	-	chr5.2:8295607-8295626	None:intergenic	40.0%
	GTTGATTAGGATGTTGGCAT+CGG	-	chr5.2:8295533-8295552	None:intergenic	40.0%
	TAGGAATCTTGTCGAAGGAT+GGG	+	chr5.2:8295392-8295411	MS.gene57158:CDS	40.0%
	TCTCTTTGTGATACCAAGCA+AGG	+	chr5.2:8295625-8295644	MS.gene57158:CDS	40.0%
	TGATACTTGACCTTTCCAGA+TGG	-	chr5.2:8295946-8295965	None:intergenic	40.0%
	TTACCAAGTGGAGTATGCAA+TGG	+	chr5.2:8295342-8295361	MS.gene57158:CDS	40.0%
	TTAGAATTGATTGTGCTGCC+TGG	-	chr5.2:8295795-8295814	None:intergenic	40.0%
!	TTTTCTTGCACCATCTGGAA+AGG	+	chr5.2:8295933-8295952	MS.gene57158:CDS	40.0%
!!	AGCAGTGTTGATTAGGATGT+TGG	-	chr5.2:8295539-8295558	None:intergenic	40.0%
	AAGCTTATCCGAGGTAAGAC+TGG	-	chr5.2:8295899-8295918	None:intergenic	45.0%
	AATTATGGCGGTTGGAAAGC+CGG	+	chr5.2:8295751-8295770	MS.gene57158:CDS	45.0%
	ACCAACTGTTCAACAGGCAT+TGG	-	chr5.2:8295601-8295620	None:intergenic	45.0%
	ACTTGGTAAAGACGTCCTTC+AGG	-	chr5.2:8295331-8295350	None:intergenic	45.0%
	AGGAATCTTGTCGAAGGATG+GGG	+	chr5.2:8295393-8295412	MS.gene57158:CDS	45.0%
	CAACCGCCATAATTTCCACT+AGG	-	chr5.2:8295745-8295764	None:intergenic	45.0%
	CAGCTTAGTCAAGTTCTCTG+GGG	-	chr5.2:8295977-8295996	None:intergenic	45.0%
	CTAGTGGAAATTATGGCGGT+TGG	+	chr5.2:8295743-8295762	MS.gene57158:CDS	45.0%
	CTCTTTCCTGTTTGCAGGAT+GGG	+	chr5.2:8295684-8295703	MS.gene57158:CDS	45.0%
	CTTTACATGAGCGATCCTAG+TGG	+	chr5.2:8295727-8295746	MS.gene57158:CDS	45.0%
	GCAGGATGGGACAAAAACTT+CGG	+	chr5.2:8295697-8295716	MS.gene57158:CDS	45.0%
	GGATAAGCTTGAACTTGCAG+AGG	+	chr5.2:8295909-8295928	MS.gene57158:CDS	45.0%
	TCTCTTTCCTGTTTGCAGGA+TGG	+	chr5.2:8295683-8295702	MS.gene57158:CDS	45.0%
	TGAAGGACGTCTTTACCAAG+TGG	+	chr5.2:8295330-8295349	MS.gene57158:CDS	45.0%
	TGCAAGTTCAAGCTTATCCG+AGG	-	chr5.2:8295908-8295927	None:intergenic	45.0%
	TGCCATAGGAATCTTGTCGA+AGG	+	chr5.2:8295387-8295406	MS.gene57158:CDS	45.0%
	TGCTCAGAACTTTCAGTGCA+AGG	-	chr5.2:8295861-8295880	None:intergenic	45.0%
	TGTTGAGGTTTGCAGAAGCT+TGG	-	chr5.2:8295449-8295468	None:intergenic	45.0%
!	ACTAAGCTGTTGGTGAAGTC+TGG	+	chr5.2:8295988-8296007	MS.gene57158:CDS	45.0%
!	ATGTTGCATGTGCTGTTGCT+GGG	+	chr5.2:8295500-8295519	MS.gene57158:CDS	45.0%
!	CTAAGCTGTTGGTGAAGTCT+GGG	+	chr5.2:8295989-8296008	MS.gene57158:CDS	45.0%
!	GAGGTTTTTCTTGCACCATC+TGG	+	chr5.2:8295928-8295947	MS.gene57158:CDS	45.0%
!	TAAGCTGTTGGTGAAGTCTG+GGG	+	chr5.2:8295990-8296009	MS.gene57158:CDS	45.0%
!	TGTTCTGGTTGGCGAAAAGA+AGG	+	chr5.2:8295417-8295436	MS.gene57158:CDS	45.0%
!	TTTTGTCCCATCCTGCAAAC+AGG	-	chr5.2:8295693-8295712	None:intergenic	45.0%
!!	GGATCCTAGCAGTGTTGATT+AGG	-	chr5.2:8295546-8295565	None:intergenic	45.0%
	AAGCAGTCTCTGTTGCTGGT+TGG	-	chr5.2:8296020-8296039	None:intergenic	50.0%
	AAGGATGGGGTTGTTCTGGT+TGG	+	chr5.2:8295406-8295425	MS.gene57158:CDS	50.0%
	ATGGAGGCCATTGGAAATGC+TGG	+	chr5.2:8295361-8295380	MS.gene57158:CDS	50.0%
	CCAAGTGGAGTATGCAATGG+AGG	+	chr5.2:8295345-8295364	MS.gene57158:CDS	50.0%
	CCTCCATTGCATACTCCACT+TGG	-	chr5.2:8295348-8295367	None:intergenic	50.0%
	GAGTATGCAATGGAGGCCAT+TGG	+	chr5.2:8295352-8295371	MS.gene57158:CDS	50.0%
	GCCAATGCCTGTTGAACAGT+TGG	+	chr5.2:8295597-8295616	MS.gene57158:CDS	50.0%
	GGAAAGAGACTCCAAACGGA+CGG	-	chr5.2:8295672-8295691	None:intergenic	50.0%
	GGAAATGCTGGTACTGCCAT+AGG	+	chr5.2:8295373-8295392	MS.gene57158:CDS	50.0%
	GGAGTCTCTTTCCTGTTTGC+AGG	+	chr5.2:8295679-8295698	MS.gene57158:CDS	50.0%
	GGCAGTACCAGCATTTCCAA+TGG	-	chr5.2:8295371-8295390	None:intergenic	50.0%
	GTTGTTGGCACCAATAGCAC+CGG	-	chr5.2:8295773-8295792	None:intergenic	50.0%
	TGATTGTGCTGCCTGGTTGT+TGG	-	chr5.2:8295788-8295807	None:intergenic	50.0%
!	AGCAGTCTCTGTTGCTGGTT+GGG	-	chr5.2:8296019-8296038	None:intergenic	50.0%
!	CATGTTGCATGTGCTGTTGC+TGG	+	chr5.2:8295499-8295518	MS.gene57158:CDS	50.0%
!!	GTTCAACAGGCATTGGCTCT+TGG	-	chr5.2:8295594-8295613	None:intergenic	50.0%
!!	TGCTATTGGTGCCAACAACC+AGG	+	chr5.2:8295774-8295793	MS.gene57158:CDS	50.0%
	GGACAGCACCAGTCTTACCT+CGG	+	chr5.2:8295888-8295907	MS.gene57158:CDS	55.0%
	GTCGAAGGATGGGGTTGTTC+TGG	+	chr5.2:8295402-8295421	MS.gene57158:CDS	55.0%
!	TTTGGTGGTCTCCGTCCGTT+TGG	+	chr5.2:8295658-8295677	MS.gene57158:CDS	55.0%
!!	GGTTGGAAAGCCGGTGCTAT+TGG	+	chr5.2:8295760-8295779	MS.gene57158:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	8295289	8296041	8295289	ID=MS.gene57158
chr5.2	mRNA	8295289	8296041	8295289	ID=MS.gene57158.t1;Parent=MS.gene57158
chr5.2	exon	8295289	8296041	8295289	ID=MS.gene57158.t1.exon1;Parent=MS.gene57158.t1
chr5.2	CDS	8295289	8296041	8295289	ID=cds.MS.gene57158.t1;Parent=MS.gene57158.t1

Gene Sequence

>MS.gene57158

ATGTCTCGAAGATATGATAGCCGTACAACAATCTTCTCTCCTGAAGGACGTCTTTACCAAGTGGAGTATGCAATGGAGGCCATTGGAAATGCTGGTACTGCCATAGGAATCTTGTCGAAGGATGGGGTTGTTCTGGTTGGCGAAAAGAAGGTGACATCCAAGCTTCTGCAAACCTCAACATCAACTGAGAAAATGTACAAGATTGATGATCATGTTGCATGTGCTGTTGCTGGGATTATGTCCGATGCCAACATCCTAATCAACACTGCTAGGATCCAAGCACAACGTTACTCATTTGCTTACCAAGAGCCAATGCCTGTTGAACAGTTGGTTCAATCTCTTTGTGATACCAAGCAAGGTTACACACAATTTGGTGGTCTCCGTCCGTTTGGAGTCTCTTTCCTGTTTGCAGGATGGGACAAAAACTTCGGCTTTCAACTTTACATGAGCGATCCTAGTGGAAATTATGGCGGTTGGAAAGCCGGTGCTATTGGTGCCAACAACCAGGCAGCACAATCAATTCTAAAACAGGACTACAAGGATGATATCACAAGGGAAGAAGCAGTTAACCTTGCACTGAAAGTTCTGAGCAAAACTATGGACAGCACCAGTCTTACCTCGGATAAGCTTGAACTTGCAGAGGTTTTTCTTGCACCATCTGGAAAGGTCAAGTATCAAGTTTGCTCCCCAGAGAACTTGACTAAGCTGTTGGTGAAGTCTGGGGTGACCCAACCAGCAACAGAGACTGCTTAG

Protein sequence

>MS.gene57158.t1

MSRRYDSRTTIFSPEGRLYQVEYAMEAIGNAGTAIGILSKDGVVLVGEKKVTSKLLQTSTSTEKMYKIDDHVACAVAGIMSDANILINTARIQAQRYSFAYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNFGFQLYMSDPSGNYGGWKAGAIGANNQAAQSILKQDYKDDITREEAVNLALKVLSKTMDSTSLTSDKLELAEVFLAPSGKVKYQVCSPENLTKLLVKSGVTQPATETA